Stard4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Stard4
Entrez Gene ID	170459
Full Name	StAR-related lipid transfer (START) domain containing 4
Synonyms	4632419C16Rik,9030213J02Rik
General protein information	Preferred Names StAR-related lipid transfer (START) domain containing 4 Names stAR-related lipid transfer protein 4 START domain-containing protein 4
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 18 Map Location: 18 B1\|18 18.18 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_133774.5	NP_598535.1	stAR-related lipid transfer protein 4 isoform 1
NM_001347624.1	NP_001334553.1	stAR-related lipid transfer protein 4 isoform 2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	STARD4	NP_001073210.1
Homo sapiens (human)	STARD4	NP_631903.1
Canis lupus familiaris (dog)	STARD4	XP_536287.3
	STARD4	XP_001101152.1
Bos taurus (cattle)	STARD4	XP_003586632.1
Danio rerio (zebrafish)	zgc:112466	NP_001017679.2
Pan troglodytes (chimpanzee)	STARD4	XP_517874.2
Mus musculus (house mouse)	Stard4	NP_598535.1
Rattus norvegicus (Norway rat)	Stard4	NP_001099629.1
Xenopus tropicalis (tropical clawed frog)	LOC100490743	XP_002943357.1

Gene Ontology
Bibliography

Related articles in PubMed

Cholesterol 7?-hydroxylase-deficient mice are protected from high-fat/high-cholesterol diet-induced metabolic disorders.
Ferrell JM, Boehme S, Li F, Chiang JY
Journal of lipid research57(7)1144-54(2016 07)

STARD4 Membrane Interactions and Sterol Binding.
Iaea DB, Dikiy I, Kiburu I, Eliezer D, Maxfield FR
Biochemistry54(30)4623-36(2015 Aug)

Cartilage-specific ablation of site-1 protease in mice results in the endoplasmic reticulum entrapment of type IIb procollagen and down-regulation of cholesterol and lipid homeostasis.
Patra D, DeLassus E, Liang G, Sandell LJ
PloS one9(8)e105674(2014)

Subcellular localization and regulation of StarD4 protein in macrophages and fibroblasts.
Rodriguez-Agudo D, Calderon-Dominguez M, Ren S, Marques D, Redford K, Medina-Torres MA, Hylemon P, Gil G, Pandak WM
Biochimica et biophysica acta1811(10)597-606(2011 Oct)

Identification of anchor genes during kidney development defines ontological relationships, molecular subcompartments and regulatory pathways.
Thiagarajan RD, Georgas KM, Rumballe BA, Lesieur E, Chiu HS, Taylor D, Tang DT, Grimmond SM, Little MH
PloS one6(2)e17286(2011 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

a model of STARD4 membrane interaction and sterol binding and release that requires dynamic movement of both the Omega1 loop and membrane insertion of the C-terminal alpha-helix.
Title: STARD4 Membrane Interactions and Sterol Binding.

StARD4's role can largely be compensated for by other intracellular cholesterol transporters.
Title: Targeted disruption of steroidogenic acute regulatory protein D4 leads to modest weight reduction and minor alterations in lipid metabolism.

StarD4 plays an important role as a directional cholesterol transporter in the maintenance of cellular cholesterol homeostasis
Title: Intracellular cholesterol transporter StarD4 binds free cholesterol and increases cholesteryl ester formation.

StarD4 is regulated by sterols via SREBP-2, and StarD5 is activated by ER stress cholesterol metabolism; they serve different functions
Title: Differential gene regulation of StarD4 and StarD5 cholesterol transfer proteins. Activation of StarD4 by sterol regulatory element-binding protein-2 and StarD5 by endoplasmic reticulum stress.

The following Stard4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Stard4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu10205	NM_133774.5 Latest version!	Mus musculus StAR-related lipid transfer (START) domain containing 4 (Stard4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu54414	NM_001347624.1 Latest version!	Mus musculus StAR-related lipid transfer (START) domain containing 4 (Stard4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Stard4 ( NM_133774.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_598535.1 Mus musculus StAR-related lipid transfer (START) domain containing 4 (Stard4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu10205
Clone ID Related Accession (Same CDS sequence)	NM_133774.5
Accession Version	NM_133774.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 675bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Mus musculus(house mouse)
Product	stAR-related lipid transfer protein 4 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB850053.1, AK085331.1 and AC114919.5. On Dec 2, 2016 this sequence version replaced NM_133774.4. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.26184.1, SRR1660813.91463.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849386 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGACC CTGAGAGCCC GTGGTCACAG ATTGGCAGGA AGATCAAACT GGAAGGACTG 
TCTGATGTTG CCTCTATTTC AACTAAACTT CAGAACACTC TGATCCAGTA TCACAGCATC 
GAAGAAGATG AGTGGCGAGT TGCCAAAAAA GCGAAAGATG TGACCGTTTG GAGAAAACCC 
TCAGAAGAGT TTAATGGGTA TCTATATAAA GCTCAAGGAG TTATGGATGA CGTGGTCAAT 
AATGTAATAG ACCATATCCG CCCAGGGCCC TGGCGCTTGG ACTGGGACCG GTTAATGACC 
TCCCTGGATG TTCTGGAGCA CTTTGAAGAG AATTGTTGTG TGATGCGTTA CACCACTGCT 
GGGCAGCTTT TAAATATTAT TTCCCCGAGA GAGTTTGTTG ATTTCTCCTA TACTGTGGGC 
TATGAAGAAG GACTTTTATC CTGTGGTGTG AGTGTTGAGT GGAGTGAGAC GAGACCAGAG 
TTTGTCCGTG GCTATAACCA TCCCTGTGGC TGGTTCTGTG TTCCACTCAA AGACAGTCCA 
AGCCAGAGTC TTCTTACAGG CTATATTCAG ACAGACCTGC GTGGCATGAT CCCTCAGTCT 
GCCGTAGATA CAGCAATGGC GAGCACGTTA GCCAACTTCT ACAGTGATTT GCGAAAAGGT 
CTACGCAAGG CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598535.1
CDS	192..866
Translation	MADPESPWSQIGRKIKLEGLSDVASISTKLQNTLIQYHSIEEDEWRVAKKAKDVTVWRKPSEEFNGYLYKAQGVMDDVVNNVIDHIRPGPWRLDWDRLMTSLDVLEHFEENCCVMRYTTAGQLLNIISPREFVDFSYTVGYEEGLLSCGVSVEWSETRPEFVRGYNHPCGWFCVPLKDSPSQSLLTGYIQTDLRGMIPQSAVDTAMASTLANFYSDLRKGLRKA

Target ORF information:

RefSeq Version	NM_133774.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus StAR-related lipid transfer (START) domain containing 4 (Stard4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133774.5

ORF Insert Sequence:

ATGGCTGACC CTGAGAGCCC GTGGTCACAG ATTGGCAGGA AGATCAAACT GGAAGGACTG 
TCTGATGTTG CCTCTATTTC AACTAAACTT CAGAACACTC TGATCCAGTA TCACAGCATC 
GAAGAAGATG AGTGGCGAGT TGCCAAAAAA GCGAAAGATG TGACCGTTTG GAGAAAACCC 
TCAGAAGAGT TTAATGGGTA TCTATATAAA GCTCAAGGAG TTATGGATGA CGTGGTCAAT 
AATGTAATAG ACCATATCCG CCCAGGGCCC TGGCGCTTGG ACTGGGACCG GTTAATGACC 
TCCCTGGATG TTCTGGAGCA CTTTGAAGAG AATTGTTGTG TGATGCGTTA CACCACTGCT 
GGGCAGCTTT TAAATATTAT TTCCCCGAGA GAGTTTGTTG ATTTCTCCTA TACTGTGGGC 
TATGAAGAAG GACTTTTATC CTGTGGTGTG AGTGTTGAGT GGAGTGAGAC GAGACCAGAG 
TTTGTCCGTG GCTATAACCA TCCCTGTGGC TGGTTCTGTG TTCCACTCAA AGACAGTCCA 
AGCCAGAGTC TTCTTACAGG CTATATTCAG ACAGACCTGC GTGGCATGAT CCCTCAGTCT 
GCCGTAGATA CAGCAATGGC GAGCACGTTA GCCAACTTCT ACAGTGATTT GCGAAAAGGT 
CTACGCAAGG CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Stard4 ( NM_001347624.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001334553.1 Mus musculus StAR-related lipid transfer (START) domain containing 4 (Stard4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu54414
Clone ID Related Accession (Same CDS sequence)	NM_001347624.1
Accession Version	NM_001347624.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Mus musculus(house mouse)
Product	stAR-related lipid transfer protein 4 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC114919.5. On Dec 2, 2016 this sequence version replaced XM_006525694.3. Transcript Variant: This variant (2) lacks an alternate exon and contains an alternate exon, resulting in a frameshift compared to variant 1. The encoded isoform (2) has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BI410943.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164135 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGACC CTGAGAGCCC GTGGTCACAG ATTGGCAGGA AGATCAAACT GGAAGGACTG 
TCTGATGTTG CCTCTATTTC AACTAAACTT CAGAACACTC TGATCCAGTA TCACAGCATC 
GAAGAAGATG AGTGGCGAGT TGCCAAAAAA GCGAAAGATG TGACCGTTTG GAGAAAACCC 
TCAGAAGAGT TTAATGGGTA TCTATATAAA GCTCAAGGAG TTATGGATGA CGTGGTCAAT 
AATGTAATAG ACCATATCCG CCCAGGGCCC TGGCGCTTGG ACTGGGACCG GTTAATGACC 
TCCCTGGATG TTCTGGAGCA CTTTGAAGAG TATCACAGAC ATTTGGGGAA GAGATCACCC 
CTTCCAGAAC ACTGTAGGCA TCCCATTCTG ATGAGAGAAG ACTGTGAGTG TCAGTTCTCA 
CGAGACATGG ATCTGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001334553.1
CDS	192..632
Translation	MADPESPWSQIGRKIKLEGLSDVASISTKLQNTLIQYHSIEEDEWRVAKKAKDVTVWRKPSEEFNGYLYKAQGVMDDVVNNVIDHIRPGPWRLDWDRLMTSLDVLEHFEEYHRHLGKRSPLPEHCRHPILMREDCECQFSRDMDLV

Target ORF information:

RefSeq Version	NM_001347624.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus StAR-related lipid transfer (START) domain containing 4 (Stard4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001347624.1

ORF Insert Sequence:

ATGGCTGACC CTGAGAGCCC GTGGTCACAG ATTGGCAGGA AGATCAAACT GGAAGGACTG 
TCTGATGTTG CCTCTATTTC AACTAAACTT CAGAACACTC TGATCCAGTA TCACAGCATC 
GAAGAAGATG AGTGGCGAGT TGCCAAAAAA GCGAAAGATG TGACCGTTTG GAGAAAACCC 
TCAGAAGAGT TTAATGGGTA TCTATATAAA GCTCAAGGAG TTATGGATGA CGTGGTCAAT 
AATGTAATAG ACCATATCCG CCCAGGGCCC TGGCGCTTGG ACTGGGACCG GTTAATGACC 
TCCCTGGATG TTCTGGAGCA CTTTGAAGAG TATCACAGAC ATTTGGGGAA GAGATCACCC 
CTTCCAGAAC ACTGTAGGCA TCCCATTCTG ATGAGAGAAG ACTGTGAGTG TCAGTTCTCA 
CGAGACATGG ATCTGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cholesterol 7?-hydroxylase-deficient mice are protected from high-fat/high-cholesterol diet-induced metabolic disorders.
Journal of lipid research57(7)1144-54(2016 07)
Ferrell JM,Boehme S,Li F,Chiang JY

STARD4 Membrane Interactions and Sterol Binding.
Biochemistry54(30)4623-36(2015 Aug)
Iaea DB,Dikiy I,Kiburu I,Eliezer D,Maxfield FR

Cartilage-specific ablation of site-1 protease in mice results in the endoplasmic reticulum entrapment of type IIb procollagen and down-regulation of cholesterol and lipid homeostasis.
PloS one9(8)e105674(2014)
Patra D,DeLassus E,Liang G,Sandell LJ

Subcellular localization and regulation of StarD4 protein in macrophages and fibroblasts.
Biochimica et biophysica acta1811(10)597-606(2011 Oct)
Rodriguez-Agudo D,Calderon-Dominguez M,Ren S,Marques D,Redford K,Medina-Torres MA,Hylemon P,Gil G,Pandak WM

Identification of anchor genes during kidney development defines ontological relationships, molecular subcompartments and regulatory pathways.
PloS one6(2)e17286(2011 Feb)
Thiagarajan RD,Georgas KM,Rumballe BA,Lesieur E,Chiu HS,Taylor D,Tang DT,Grimmond SM,Little MH