Stt3a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Stt3a
Entrez Gene ID	16430
Full Name	STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae)
Synonyms	AA408947,BB081708,Itm1
General protein information	Preferred Names STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) Names dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A B5 STT3-A integral membrane protein 1 intergral membrane protein 1 oligosaccharyl transferase subunit STT3A
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9 A4\|9 20.67 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_008408.4	NP_032434.3	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A
NM_008408.5	NP_032434.3	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A
XM_030244074.1	XP_030099934.1	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A isoform X1
XM_006510038.3	XP_006510101.1	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Stt3a	NP_032434.3
Drosophila melanogaster (fruit fly)	CG1518	NP_608425.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000434	XP_310665.3
Xenopus tropicalis (tropical clawed frog)	stt3a	NP_001001202.1
	STT3A	XP_002799889.1
Canis lupus familiaris (dog)	STT3A	XP_546418.3
Bos taurus (cattle)	STT3A	NP_001039445.1
Arabidopsis thaliana (thale cress)	STT3B	NP_174675.2
Gallus gallus (chicken)	LOC100857165	XP_004948012.1
Oryza sativa (rice)	Os04g0675500	NP_001054248.1
Homo sapiens (human)	STT3A	NP_689926.1
Pan troglodytes (chimpanzee)	STT3A	NP_001233378.1
Rattus norvegicus (Norway rat)	Stt3a	NP_001128221.1
Danio rerio (zebrafish)	itm1	NP_958866.1

Gene Ontology
Bibliography

Related articles in PubMed

Refined topology model of the STT3/Stt3 protein subunit of the oligosaccharyltransferase complex.
Lara P, ?jemalm K, Reithinger J, Holgado A, Maojun Y, Hammed A, Mattle D, Kim H, Nilsson I
The Journal of biological chemistry292(27)11349-11360(2017 07)

Widespread and dynamic translational control of red blood cell development.
Alvarez-Dominguez JR, Zhang X, Hu W
Blood129(5)619-629(2017 02)

Microarray analysis of Foxa2 mutant mouse embryos reveals novel gene expression and inductive roles for the gastrula organizer and its derivatives.
Tamplin OJ, Kinzel D, Cox BJ, Bell CE, Rossant J, Lickert H
BMC genomics9511(2008 Oct)

Proteomic analysis of mammalian oligosaccharyltransferase reveals multiple subcomplexes that contain Sec61, TRAP, and two potential new subunits.
Shibatani T, David LL, McCormack AL, Frueh K, Skach WR
Biochemistry44(16)5982-92(2005 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results show that most of the STT3 TMDs are well inserted into the ER membrane on their own or in the presence of the natural flanking residues.
Title: Refined topology model of the STT3/Stt3 protein subunit of the oligosaccharyltransferase complex.

The following Stt3a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Stt3a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu01875	NM_008408.5 Latest version!	Mus musculus STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) (Stt3a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$419.30 $599.00
OMu42022	XM_006510038.3 Latest version!	Mus musculus STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) (Stt3a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu42022	XM_030244074.1 Latest version!	Mus musculus STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) (Stt3a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu01875	NM_008408.4	Mus musculus STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) (Stt3a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$419.30 $599.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Stt3a ( NM_008408.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032434.3 Mus musculus STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) (Stt3a), mRNA.

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CloneID	OMu01875
Clone ID Related Accession (Same CDS sequence)	NM_008408.4 , NM_008408.5
Accession Version	NM_008408.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2118bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Mus musculus(house mouse)
Product	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK153840.1. On Jun 10, 2007 this sequence version replaced NM_008408.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK151541.1, AK151791.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164135 [ECO:0000348] ##Evidence-Data-END##

ATGACTAAGC TTGGATTTTT GCGATTGTCC TATGAGAAGC AGGACACACT TCTAAAGCTT 
CTCATCCTGT CGATGGCTGC TGTGTTATCT TTTTCTACTC GTCTTTTTGC TGTGCTGAGA 
TTTGAAAGTG TCATCCATGA GTTTGATCCG TACTTTAATT ATCGGACTAC CCGGTTTCTG 
GCTGAGGAGG GGTTTTATAA ATTCCATAAC TGGTTTGATG ACCGGGCTTG GTACCCTTTG 
GGCCGAATCA TTGGAGGAAC AATTTACCCA GGTTTAATGA TCACTTCTGC TGCAATCTAC 
CATGTACTCC ATTTCTTCCA TATCACCATT GACATTCGGA ATGTCTGTGT TTTCCTGGCC 
CCGCTTTTCT CCTCTTTCAC CACCATCGTT ACGTACCACC TTACCAAAGA GCTCAAGGAT 
GCAGGAGCTG GGCTTCTTGC TGCTGCCATG ATTGCTGTAG TTCCTGGGTA TATTTCTCGA 
TCTGTAGCTG GCTCCTATGA TAATGAAGGA ATTGCTATCT TTTGCATGCT GCTTACTTAC 
TACATGTGGA TCAAGGCAGT GAAGACTGGT TCCATCTATT GGGCTGCCAA GTGTGCCCTC 
GCTTATTTCT ACATGGTCTC TTCATGGGGA GGCTATGTGT TCCTGATCAA CTTGATTCCT 
CTACATGTCC TGGTGCTAAT GCTGACAGGC CGTTTTTCTC ACCGGATCTA CGTAGCCTAC 
TGTACTGTTT ACTGCCTGGG CACCATTCTT TCTATGCAGA TTTCCTTTGT TGGTTTCCAG 
CCCGTCCTTT CATCAGAACA CATGGCAGCC TTTGGAGTGT TTGGTCTCTG TCAGATCCAT 
GCTTTCGTAG ATTACCTGCG CAGCAAGTTG AATCCACAGC AATTCGAAGT TCTTTTCCGG 
AGTGTTATCT CCCTGGTTGG CTTTGTCCTC CTCACTGTGG GAGCTCTCCT CATGCTAACA 
GGAAAAATTT CTCCCTGGAC AGGGCGTTTC TACTCTCTGC TGGATCCCTC TTATGCTAAG 
AATAACATTC CCATTATTGC ATCTGTTTCT GAGCACCAGC CCACAACCTG GTCTTCCTAC 
TATTTTGATC TACAGCTCCT TGTCTTCATG TTTCCAGTTG GCCTCTATTA CTGCTTTAGC 
AACCTGTCTG ATGCTCGGAT TTTTATCATC ATGTATGGTG TGACCAGCAT GTACTTTTCA 
GCTGTAATGG TGCGTCTAAT GCTGGTATTG GCACCTGTTA TGTGCATTCT TTCTGGCATT 
GGTGTTTCCC AGGTGCTGTC CACATATATG AAAAATCTGG ACATAAGTCG CCCAGACAAG 
AAGAGCAAGA AGCAACAGGA TTCTACTTAC CCTATTAAGA ATGAGGTGGC GAGTGGGATG 
ATACTGGTCA TGGCTTTCTT TCTCATCACC TACACGTTTC ATTCGACTTG GGTGACCAGT 
GAAGCCTATT CTTCTCCCTC CATTGTTCTG TCTGCTCGTG GTGGGGATGG CAGTAGGATC 
ATTTTTGATG ACTTCCGAGA AGCGTATTAT TGGCTCCGTC ACAATACTCC AGAGGATGCA 
AAAGTCATGT CATGGTGGGA TTATGGCTAC CAAATTACTG CAATGGCAAA TCGGACAATT 
TTAGTGGACA ATAACACATG GAATAATACC CATATTTCTC GAGTAGGGCA GGCAATGGCA 
TCCACAGAAG AAAAAGCCTA TGAAATCATG AGGGAGCTTG ATGTCAGCTA TGTGCTTGTC 
ATTTTTGGAG GCCTTACTGG GTATTCTTCG GATGATATCA ACAAGTTTCT TTGGATGGTC 
CGGATTGGAG GAAGCACAGA GACAGGAAGA CACATTAAGG AGAATGACTA CTATACTCCT 
ACTGGGGAAT TCCGTGTTGA TCGTGAGGGT TCTCCGGTGC TGCTCAACTG CCTTATGTAC 
AAAATGTGTT ACTACCGCTT TGGGCAGGTC TACACAGAAG CCAAGCGTCC ACCAGGCTTT 
GACCGTGTTC GAAATGCTGA GATTGGTAAT AAAGACTTTG AGCTTGATGT CCTGGAGGAA 
GCGTATACCA CAGAACACTG GCTAGTCAGG ATATACAAGG TAAAGGACCT GGATAATCGA 
GGCTTGTCAA GGACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032434.3
CDS	69..2186
Translation	MTKLGFLRLSYEKQDTLLKLLILSMAAVLSFSTRLFAVLRFESVIHEFDPYFNYRTTRFLAEEGFYKFHNWFDDRAWYPLGRIIGGTIYPGLMITSAAIYHVLHFFHITIDIRNVCVFLAPLFSSFTTIVTYHLTKELKDAGAGLLAAAMIAVVPGYISRSVAGSYDNEGIAIFCMLLTYYMWIKAVKTGSIYWAAKCALAYFYMVSSWGGYVFLINLIPLHVLVLMLTGRFSHRIYVAYCTVYCLGTILSMQISFVGFQPVLSSEHMAAFGVFGLCQIHAFVDYLRSKLNPQQFEVLFRSVISLVGFVLLTVGALLMLTGKISPWTGRFYSLLDPSYAKNNIPIIASVSEHQPTTWSSYYFDLQLLVFMFPVGLYYCFSNLSDARIFIIMYGVTSMYFSAVMVRLMLVLAPVMCILSGIGVSQVLSTYMKNLDISRPDKKSKKQQDSTYPIKNEVASGMILVMAFFLITYTFHSTWVTSEAYSSPSIVLSARGGDGSRIIFDDFREAYYWLRHNTPEDAKVMSWWDYGYQITAMANRTILVDNNTWNNTHISRVGQAMASTEEKAYEIMRELDVSYVLVIFGGLTGYSSDDINKFLWMVRIGGSTETGRHIKENDYYTPTGEFRVDREGSPVLLNCLMYKMCYYRFGQVYTEAKRPPGFDRVRNAEIGNKDFELDVLEEAYTTEHWLVRIYKVKDLDNRGLSRT

Target ORF information:

RefSeq Version	NM_008408.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) (Stt3a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008408.4

ORF Insert Sequence:

ATGACTAAGC TTGGATTTTT GCGATTGTCC TATGAGAAGC AGGACACACT TCTAAAGCTT 
CTCATCCTGT CGATGGCTGC TGTGTTATCT TTTTCTACTC GTCTTTTTGC TGTGCTGAGA 
TTTGAAAGTG TCATCCATGA GTTTGATCCG TACTTTAATT ATCGGACTAC CCGGTTTCTG 
GCTGAGGAGG GGTTTTATAA ATTCCATAAC TGGTTTGATG ACCGGGCTTG GTACCCTTTG 
GGCCGAATCA TTGGAGGAAC AATTTACCCA GGTTTAATGA TCACTTCTGC TGCAATCTAC 
CATGTACTCC ATTTCTTCCA TATCACCATT GACATTCGGA ATGTCTGTGT TTTCCTGGCC 
CCGCTTTTCT CCTCTTTCAC CACCATCGTT ACGTACCACC TTACCAAAGA GCTCAAGGAT 
GCAGGAGCTG GGCTTCTTGC TGCTGCCATG ATTGCTGTAG TTCCTGGGTA TATTTCTCGA 
TCTGTAGCTG GCTCCTATGA TAATGAAGGA ATTGCTATCT TTTGCATGCT GCTTACTTAC 
TACATGTGGA TCAAGGCAGT GAAGACTGGT TCCATCTATT GGGCTGCCAA GTGTGCCCTC 
GCTTATTTCT ACATGGTCTC TTCATGGGGA GGCTATGTGT TCCTGATCAA CTTGATTCCT 
CTACATGTCC TGGTGCTAAT GCTGACAGGC CGTTTTTCTC ACCGGATCTA CGTAGCCTAC 
TGTACTGTTT ACTGCCTGGG CACCATTCTT TCTATGCAGA TTTCCTTTGT TGGTTTCCAG 
CCCGTCCTTT CATCAGAACA CATGGCAGCC TTTGGAGTGT TTGGTCTCTG TCAGATCCAT 
GCTTTCGTAG ATTACCTGCG CAGCAAGTTG AATCCACAGC AATTCGAAGT TCTTTTCCGG 
AGTGTTATCT CCCTGGTTGG CTTTGTCCTC CTCACTGTGG GAGCTCTCCT CATGCTAACA 
GGAAAAATTT CTCCCTGGAC AGGGCGTTTC TACTCTCTGC TGGATCCCTC TTATGCTAAG 
AATAACATTC CCATTATTGC ATCTGTTTCT GAGCACCAGC CCACAACCTG GTCTTCCTAC 
TATTTTGATC TACAGCTCCT TGTCTTCATG TTTCCAGTTG GCCTCTATTA CTGCTTTAGC 
AACCTGTCTG ATGCTCGGAT TTTTATCATC ATGTATGGTG TGACCAGCAT GTACTTTTCA 
GCTGTAATGG TGCGTCTAAT GCTGGTATTG GCACCTGTTA TGTGCATTCT TTCTGGCATT 
GGTGTTTCCC AGGTGCTGTC CACATATATG AAAAATCTGG ACATAAGTCG CCCAGACAAG 
AAGAGCAAGA AGCAACAGGA TTCTACTTAC CCTATTAAGA ATGAGGTGGC GAGTGGGATG 
ATACTGGTCA TGGCTTTCTT TCTCATCACC TACACGTTTC ATTCGACTTG GGTGACCAGT 
GAAGCCTATT CTTCTCCCTC CATTGTTCTG TCTGCTCGTG GTGGGGATGG CAGTAGGATC 
ATTTTTGATG ACTTCCGAGA AGCGTATTAT TGGCTCCGTC ACAATACTCC AGAGGATGCA 
AAAGTCATGT CATGGTGGGA TTATGGCTAC CAAATTACTG CAATGGCAAA TCGGACAATT 
TTAGTGGACA ATAACACATG GAATAATACC CATATTTCTC GAGTAGGGCA GGCAATGGCA 
TCCACAGAAG AAAAAGCCTA TGAAATCATG AGGGAGCTTG ATGTCAGCTA TGTGCTTGTC 
ATTTTTGGAG GCCTTACTGG GTATTCTTCG GATGATATCA ACAAGTTTCT TTGGATGGTC 
CGGATTGGAG GAAGCACAGA GACAGGAAGA CACATTAAGG AGAATGACTA CTATACTCCT 
ACTGGGGAAT TCCGTGTTGA TCGTGAGGGT TCTCCGGTGC TGCTCAACTG CCTTATGTAC 
AAAATGTGTT ACTACCGCTT TGGGCAGGTC TACACAGAAG CCAAGCGTCC ACCAGGCTTT 
GACCGTGTTC GAAATGCTGA GATTGGTAAT AAAGACTTTG AGCTTGATGT CCTGGAGGAA 
GCGTATACCA CAGAACACTG GCTAGTCAGG ATATACAAGG TAAAGGACCT GGATAATCGA 
GGCTTGTCAA GGACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Stt3a ( NM_008408.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032434.3 Mus musculus STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) (Stt3a), mRNA.

Price & Availability↑

CloneID	OMu01875
Clone ID Related Accession (Same CDS sequence)	NM_008408.4 , NM_008408.5
Accession Version	NM_008408.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2118bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Mus musculus(house mouse)
Product	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK150640.1, AK153840.1, AK050050.1 and AC155921.2. On Jun 19, 2018 this sequence version replaced NM_008408.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK168937.1, AK152242.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTAAGC TTGGATTTTT GCGATTGTCC TATGAGAAGC AGGACACACT TCTAAAGCTT 
CTCATCCTGT CGATGGCTGC TGTGTTATCT TTTTCTACTC GTCTTTTTGC TGTGCTGAGA 
TTTGAAAGTG TCATCCATGA GTTTGATCCG TACTTTAATT ATCGGACTAC CCGGTTTCTG 
GCTGAGGAGG GGTTTTATAA ATTCCATAAC TGGTTTGATG ACCGGGCTTG GTACCCTTTG 
GGCCGAATCA TTGGAGGAAC AATTTACCCA GGTTTAATGA TCACTTCTGC TGCAATCTAC 
CATGTACTCC ATTTCTTCCA TATCACCATT GACATTCGGA ATGTCTGTGT TTTCCTGGCC 
CCGCTTTTCT CCTCTTTCAC CACCATCGTT ACGTACCACC TTACCAAAGA GCTCAAGGAT 
GCAGGAGCTG GGCTTCTTGC TGCTGCCATG ATTGCTGTAG TTCCTGGGTA TATTTCTCGA 
TCTGTAGCTG GCTCCTATGA TAATGAAGGA ATTGCTATCT TTTGCATGCT GCTTACTTAC 
TACATGTGGA TCAAGGCAGT GAAGACTGGT TCCATCTATT GGGCTGCCAA GTGTGCCCTC 
GCTTATTTCT ACATGGTCTC TTCATGGGGA GGCTATGTGT TCCTGATCAA CTTGATTCCT 
CTACATGTCC TGGTGCTAAT GCTGACAGGC CGTTTTTCTC ACCGGATCTA CGTAGCCTAC 
TGTACTGTTT ACTGCCTGGG CACCATTCTT TCTATGCAGA TTTCCTTTGT TGGTTTCCAG 
CCCGTCCTTT CATCAGAACA CATGGCAGCC TTTGGAGTGT TTGGTCTCTG TCAGATCCAT 
GCTTTCGTAG ATTACCTGCG CAGCAAGTTG AATCCACAGC AATTCGAAGT TCTTTTCCGG 
AGTGTTATCT CCCTGGTTGG CTTTGTCCTC CTCACTGTGG GAGCTCTCCT CATGCTAACA 
GGAAAAATTT CTCCCTGGAC AGGGCGTTTC TACTCTCTGC TGGATCCCTC TTATGCTAAG 
AATAACATTC CCATTATTGC ATCTGTTTCT GAGCACCAGC CCACAACCTG GTCTTCCTAC 
TATTTTGATC TACAGCTCCT TGTCTTCATG TTTCCAGTTG GCCTCTATTA CTGCTTTAGC 
AACCTGTCTG ATGCTCGGAT TTTTATCATC ATGTATGGTG TGACCAGCAT GTACTTTTCA 
GCTGTAATGG TGCGTCTAAT GCTGGTATTG GCACCTGTTA TGTGCATTCT TTCTGGCATT 
GGTGTTTCCC AGGTGCTGTC CACATATATG AAAAATCTGG ACATAAGTCG CCCAGACAAG 
AAGAGCAAGA AGCAACAGGA TTCTACTTAC CCTATTAAGA ATGAGGTGGC GAGTGGGATG 
ATACTGGTCA TGGCTTTCTT TCTCATCACC TACACGTTTC ATTCGACTTG GGTGACCAGT 
GAAGCCTATT CTTCTCCCTC CATTGTTCTG TCTGCTCGTG GTGGGGATGG CAGTAGGATC 
ATTTTTGATG ACTTCCGAGA AGCGTATTAT TGGCTCCGTC ACAATACTCC AGAGGATGCA 
AAAGTCATGT CATGGTGGGA TTATGGCTAC CAAATTACTG CAATGGCAAA TCGGACAATT 
TTAGTGGACA ATAACACATG GAATAATACC CATATTTCTC GAGTAGGGCA GGCAATGGCA 
TCCACAGAAG AAAAAGCCTA TGAAATCATG AGGGAGCTTG ATGTCAGCTA TGTGCTTGTC 
ATTTTTGGAG GCCTTACTGG GTATTCTTCG GATGATATCA ACAAGTTTCT TTGGATGGTC 
CGGATTGGAG GAAGCACAGA GACAGGAAGA CACATTAAGG AGAATGACTA CTATACTCCT 
ACTGGGGAAT TCCGTGTTGA TCGTGAGGGT TCTCCGGTGC TGCTCAACTG CCTTATGTAC 
AAAATGTGTT ACTACCGCTT TGGGCAGGTC TACACAGAAG CCAAGCGTCC ACCAGGCTTT 
GACCGTGTTC GAAATGCTGA GATTGGTAAT AAAGACTTTG AGCTTGATGT CCTGGAGGAA 
GCGTATACCA CAGAACACTG GCTAGTCAGG ATATACAAGG TAAAGGACCT GGATAATCGA 
GGCTTGTCAA GGACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032434.3
CDS	113..2230
Translation	MTKLGFLRLSYEKQDTLLKLLILSMAAVLSFSTRLFAVLRFESVIHEFDPYFNYRTTRFLAEEGFYKFHNWFDDRAWYPLGRIIGGTIYPGLMITSAAIYHVLHFFHITIDIRNVCVFLAPLFSSFTTIVTYHLTKELKDAGAGLLAAAMIAVVPGYISRSVAGSYDNEGIAIFCMLLTYYMWIKAVKTGSIYWAAKCALAYFYMVSSWGGYVFLINLIPLHVLVLMLTGRFSHRIYVAYCTVYCLGTILSMQISFVGFQPVLSSEHMAAFGVFGLCQIHAFVDYLRSKLNPQQFEVLFRSVISLVGFVLLTVGALLMLTGKISPWTGRFYSLLDPSYAKNNIPIIASVSEHQPTTWSSYYFDLQLLVFMFPVGLYYCFSNLSDARIFIIMYGVTSMYFSAVMVRLMLVLAPVMCILSGIGVSQVLSTYMKNLDISRPDKKSKKQQDSTYPIKNEVASGMILVMAFFLITYTFHSTWVTSEAYSSPSIVLSARGGDGSRIIFDDFREAYYWLRHNTPEDAKVMSWWDYGYQITAMANRTILVDNNTWNNTHISRVGQAMASTEEKAYEIMRELDVSYVLVIFGGLTGYSSDDINKFLWMVRIGGSTETGRHIKENDYYTPTGEFRVDREGSPVLLNCLMYKMCYYRFGQVYTEAKRPPGFDRVRNAEIGNKDFELDVLEEAYTTEHWLVRIYKVKDLDNRGLSRT

Target ORF information:

RefSeq Version	NM_008408.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) (Stt3a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008408.5

ORF Insert Sequence:

ATGACTAAGC TTGGATTTTT GCGATTGTCC TATGAGAAGC AGGACACACT TCTAAAGCTT 
CTCATCCTGT CGATGGCTGC TGTGTTATCT TTTTCTACTC GTCTTTTTGC TGTGCTGAGA 
TTTGAAAGTG TCATCCATGA GTTTGATCCG TACTTTAATT ATCGGACTAC CCGGTTTCTG 
GCTGAGGAGG GGTTTTATAA ATTCCATAAC TGGTTTGATG ACCGGGCTTG GTACCCTTTG 
GGCCGAATCA TTGGAGGAAC AATTTACCCA GGTTTAATGA TCACTTCTGC TGCAATCTAC 
CATGTACTCC ATTTCTTCCA TATCACCATT GACATTCGGA ATGTCTGTGT TTTCCTGGCC 
CCGCTTTTCT CCTCTTTCAC CACCATCGTT ACGTACCACC TTACCAAAGA GCTCAAGGAT 
GCAGGAGCTG GGCTTCTTGC TGCTGCCATG ATTGCTGTAG TTCCTGGGTA TATTTCTCGA 
TCTGTAGCTG GCTCCTATGA TAATGAAGGA ATTGCTATCT TTTGCATGCT GCTTACTTAC 
TACATGTGGA TCAAGGCAGT GAAGACTGGT TCCATCTATT GGGCTGCCAA GTGTGCCCTC 
GCTTATTTCT ACATGGTCTC TTCATGGGGA GGCTATGTGT TCCTGATCAA CTTGATTCCT 
CTACATGTCC TGGTGCTAAT GCTGACAGGC CGTTTTTCTC ACCGGATCTA CGTAGCCTAC 
TGTACTGTTT ACTGCCTGGG CACCATTCTT TCTATGCAGA TTTCCTTTGT TGGTTTCCAG 
CCCGTCCTTT CATCAGAACA CATGGCAGCC TTTGGAGTGT TTGGTCTCTG TCAGATCCAT 
GCTTTCGTAG ATTACCTGCG CAGCAAGTTG AATCCACAGC AATTCGAAGT TCTTTTCCGG 
AGTGTTATCT CCCTGGTTGG CTTTGTCCTC CTCACTGTGG GAGCTCTCCT CATGCTAACA 
GGAAAAATTT CTCCCTGGAC AGGGCGTTTC TACTCTCTGC TGGATCCCTC TTATGCTAAG 
AATAACATTC CCATTATTGC ATCTGTTTCT GAGCACCAGC CCACAACCTG GTCTTCCTAC 
TATTTTGATC TACAGCTCCT TGTCTTCATG TTTCCAGTTG GCCTCTATTA CTGCTTTAGC 
AACCTGTCTG ATGCTCGGAT TTTTATCATC ATGTATGGTG TGACCAGCAT GTACTTTTCA 
GCTGTAATGG TGCGTCTAAT GCTGGTATTG GCACCTGTTA TGTGCATTCT TTCTGGCATT 
GGTGTTTCCC AGGTGCTGTC CACATATATG AAAAATCTGG ACATAAGTCG CCCAGACAAG 
AAGAGCAAGA AGCAACAGGA TTCTACTTAC CCTATTAAGA ATGAGGTGGC GAGTGGGATG 
ATACTGGTCA TGGCTTTCTT TCTCATCACC TACACGTTTC ATTCGACTTG GGTGACCAGT 
GAAGCCTATT CTTCTCCCTC CATTGTTCTG TCTGCTCGTG GTGGGGATGG CAGTAGGATC 
ATTTTTGATG ACTTCCGAGA AGCGTATTAT TGGCTCCGTC ACAATACTCC AGAGGATGCA 
AAAGTCATGT CATGGTGGGA TTATGGCTAC CAAATTACTG CAATGGCAAA TCGGACAATT 
TTAGTGGACA ATAACACATG GAATAATACC CATATTTCTC GAGTAGGGCA GGCAATGGCA 
TCCACAGAAG AAAAAGCCTA TGAAATCATG AGGGAGCTTG ATGTCAGCTA TGTGCTTGTC 
ATTTTTGGAG GCCTTACTGG GTATTCTTCG GATGATATCA ACAAGTTTCT TTGGATGGTC 
CGGATTGGAG GAAGCACAGA GACAGGAAGA CACATTAAGG AGAATGACTA CTATACTCCT 
ACTGGGGAAT TCCGTGTTGA TCGTGAGGGT TCTCCGGTGC TGCTCAACTG CCTTATGTAC 
AAAATGTGTT ACTACCGCTT TGGGCAGGTC TACACAGAAG CCAAGCGTCC ACCAGGCTTT 
GACCGTGTTC GAAATGCTGA GATTGGTAAT AAAGACTTTG AGCTTGATGT CCTGGAGGAA 
GCGTATACCA CAGAACACTG GCTAGTCAGG ATATACAAGG TAAAGGACCT GGATAATCGA 
GGCTTGTCAA GGACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Stt3a ( XM_006510038.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510101.1 Mus musculus STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) (Stt3a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu42022
Clone ID Related Accession (Same CDS sequence)	XM_030244074.1 , XM_006510038.3
Accession Version	XM_006510038.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006510038.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGAGCG GGCAGGGAGG CGGGCCTTGG CTGCTGTCAG GGTTGAGTGC GCCGCTGAAC 
GGATGGCAGG GGGAGCAGAG TGGGTTCCTG AGGAGCATCC GTGAGGTATT TGAATATCAT 
CAGTTGCCAC CCATTGATGT CAAGATGACT AAGCTTGGAT TTTTGCGATT GTCCTATGAG 
AAGCAGGACA CACTTCTAAA GCTTCTCATC CTGTCGATGG CTGCTGTGTT ATCTTTTTCT 
ACTCGTCTTT TTGCTGTGCT GAGATTTGAA AGTGTCATCC ATGAGTTTGA TCCGTACTTT 
AATTATCGGA CTACCCGGTT TCTGGCTGAG GAGGGGTTTT ATAAATTCCA TAACTGGTTT 
GATGACCGGG CTTGGTACCC TTTGGGCCGA ATCATTGGAG GAACAATTTA CCCAGGTTTA 
ATGATCACTT CTGCTGCAAT CTACCATGTA CTCCATTTCT TCCATATCAC CATTGACATT 
CGGAATGTCT GTGTTTTCCT GGCCCCGCTT TTCTCCTCTT TCACCACCAT CGTTACGTAC 
CACCTTACCA AAGAGCTCAA GGATGCAGGA GCTGGGCTTC TTGCTGCTGC CATGATTGCT 
GTAGTTCCTG GGTATATTTC TCGATCTGTA GCTGGCTCCT ATGATAATGA AGGAATTGCT 
ATCTTTTGCA TGCTGCTTAC TTACTACATG TGGATCAAGG CAGTGAAGAC TGGTTCCATC 
TATTGGGCTG CCAAGTGTGC CCTCGCTTAT TTCTACATGG TCTCTTCATG GGGAGGCTAT 
GTGTTCCTGA TCAACTTGAT TCCTCTACAT GTCCTGGTGC TAATGCTGAC AGGCCGTTTT 
TCTCACCGGA TCTACGTAGC CTACTGTACT GTTTACTGCC TGGGCACCAT TCTTTCTATG 
CAGATTTCCT TTGTTGGTTT CCAGCCCGTC CTTTCATCAG AACACATGGC AGCCTTTGGA 
GTGTTTGGTC TCTGTCAGAT CCATGCTTTC GTAGATTACC TGCGCAGCAA GTTGAATCCA 
CAGCAATTCG AAGTTCTTTT CCGGAGTGTT ATCTCCCTGG TTGGCTTTGT CCTCCTCACT 
GTGGGAGCTC TCCTCATGCT AACAGGAAAA ATTTCTCCCT GGACAGGGCG TTTCTACTCT 
CTGCTGGATC CCTCTTATGC TAAGAATAAC ATTCCCATTA TTGCATCTGT TTCTGAGCAC 
CAGCCCACAA CCTGGTCTTC CTACTATTTT GATCTACAGC TCCTTGTCTT CATGTTTCCA 
GTTGGCCTCT ATTACTGCTT TAGCAACCTG TCTGATGCTC GGATTTTTAT CATCATGTAT 
GGTGTGACCA GCATGTACTT TTCAGCTGTA ATGGTGCGTC TAATGCTGGT ATTGGCACCT 
GTTATGTGCA TTCTTTCTGG CATTGGTGTT TCCCAGGTGC TGTCCACATA TATGAAAAAT 
CTGGACATAA GTCGCCCAGA CAAGAAGAGC AAGAAGCAAC AGGATTCTAC TTACCCTATT 
AAGAATGAGG TGGCGAGTGG GATGATACTG GTCATGGCTT TCTTTCTCAT CACCTACACG 
TTTCATTCGA CTTGGGTGAC CAGTGAAGCC TATTCTTCTC CCTCCATTGT TCTGTCTGCT 
CGTGGTGGGG ATGGCAGTAG GATCATTTTT GATGACTTCC GAGAAGCGTA TTATTGGCTC 
CGTCACAATA CTCCAGAGGA TGCAAAAGTC ATGTCATGGT GGGATTATGG CTACCAAATT 
ACTGCAATGG CAAATCGGAC AATTTTAGTG GACAATAACA CATGGAATAA TACCCATATT 
TCTCGAGTAG GGCAGGCAAT GGCATCCACA GAAGAAAAAG CCTATGAAAT CATGAGGGAG 
CTTGATGTCA GCTATGTGCT TGTCATTTTT GGAGGCCTTA CTGGGTATTC TTCGGATGAT 
ATCAACAAGT TTCTTTGGAT GGTCCGGATT GGAGGAAGCA CAGAGACAGG AAGACACATT 
AAGGAGAATG ACTACTATAC TCCTACTGGG GAATTCCGTG TTGATCGTGA GGGTTCTCCG 
GTGCTGCTCA ACTGCCTTAT GTACAAAATG TGTTACTACC GCTTTGGGCA GGTCTACACA 
GAAGCCAAGC GTCCACCAGG CTTTGACCGT GTTCGAAATG CTGAGATTGG TAATAAAGAC 
TTTGAGCTTG ATGTCCTGGA GGAAGCGTAT ACCACAGAAC ACTGGCTAGT CAGGATATAC 
AAGGTAAAGG ACCTGGATAA TCGAGGCTTG TCAAGGACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510101.1
CDS	26..2287
Translation	MRSGQGGGPWLLSGLSAPLNGWQGEQSGFLRSIREVFEYHQLPPIDVKMTKLGFLRLSYEKQDTLLKLLILSMAAVLSFSTRLFAVLRFESVIHEFDPYFNYRTTRFLAEEGFYKFHNWFDDRAWYPLGRIIGGTIYPGLMITSAAIYHVLHFFHITIDIRNVCVFLAPLFSSFTTIVTYHLTKELKDAGAGLLAAAMIAVVPGYISRSVAGSYDNEGIAIFCMLLTYYMWIKAVKTGSIYWAAKCALAYFYMVSSWGGYVFLINLIPLHVLVLMLTGRFSHRIYVAYCTVYCLGTILSMQISFVGFQPVLSSEHMAAFGVFGLCQIHAFVDYLRSKLNPQQFEVLFRSVISLVGFVLLTVGALLMLTGKISPWTGRFYSLLDPSYAKNNIPIIASVSEHQPTTWSSYYFDLQLLVFMFPVGLYYCFSNLSDARIFIIMYGVTSMYFSAVMVRLMLVLAPVMCILSGIGVSQVLSTYMKNLDISRPDKKSKKQQDSTYPIKNEVASGMILVMAFFLITYTFHSTWVTSEAYSSPSIVLSARGGDGSRIIFDDFREAYYWLRHNTPEDAKVMSWWDYGYQITAMANRTILVDNNTWNNTHISRVGQAMASTEEKAYEIMRELDVSYVLVIFGGLTGYSSDDINKFLWMVRIGGSTETGRHIKENDYYTPTGEFRVDREGSPVLLNCLMYKMCYYRFGQVYTEAKRPPGFDRVRNAEIGNKDFELDVLEEAYTTEHWLVRIYKVKDLDNRGLSRT

Target ORF information:

RefSeq Version	XM_006510038.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) (Stt3a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510038.3

ORF Insert Sequence:

ATGCGGAGCG GGCAGGGAGG CGGGCCTTGG CTGCTGTCAG GGTTGAGTGC GCCGCTGAAC 
GGATGGCAGG GGGAGCAGAG TGGGTTCCTG AGGAGCATCC GTGAGGTATT TGAATATCAT 
CAGTTGCCAC CCATTGATGT CAAGATGACT AAGCTTGGAT TTTTGCGATT GTCCTATGAG 
AAGCAGGACA CACTTCTAAA GCTTCTCATC CTGTCGATGG CTGCTGTGTT ATCTTTTTCT 
ACTCGTCTTT TTGCTGTGCT GAGATTTGAA AGTGTCATCC ATGAGTTTGA TCCGTACTTT 
AATTATCGGA CTACCCGGTT TCTGGCTGAG GAGGGGTTTT ATAAATTCCA TAACTGGTTT 
GATGACCGGG CTTGGTACCC TTTGGGCCGA ATCATTGGAG GAACAATTTA CCCAGGTTTA 
ATGATCACTT CTGCTGCAAT CTACCATGTA CTCCATTTCT TCCATATCAC CATTGACATT 
CGGAATGTCT GTGTTTTCCT GGCCCCGCTT TTCTCCTCTT TCACCACCAT CGTTACGTAC 
CACCTTACCA AAGAGCTCAA GGATGCAGGA GCTGGGCTTC TTGCTGCTGC CATGATTGCT 
GTAGTTCCTG GGTATATTTC TCGATCTGTA GCTGGCTCCT ATGATAATGA AGGAATTGCT 
ATCTTTTGCA TGCTGCTTAC TTACTACATG TGGATCAAGG CAGTGAAGAC TGGTTCCATC 
TATTGGGCTG CCAAGTGTGC CCTCGCTTAT TTCTACATGG TCTCTTCATG GGGAGGCTAT 
GTGTTCCTGA TCAACTTGAT TCCTCTACAT GTCCTGGTGC TAATGCTGAC AGGCCGTTTT 
TCTCACCGGA TCTACGTAGC CTACTGTACT GTTTACTGCC TGGGCACCAT TCTTTCTATG 
CAGATTTCCT TTGTTGGTTT CCAGCCCGTC CTTTCATCAG AACACATGGC AGCCTTTGGA 
GTGTTTGGTC TCTGTCAGAT CCATGCTTTC GTAGATTACC TGCGCAGCAA GTTGAATCCA 
CAGCAATTCG AAGTTCTTTT CCGGAGTGTT ATCTCCCTGG TTGGCTTTGT CCTCCTCACT 
GTGGGAGCTC TCCTCATGCT AACAGGAAAA ATTTCTCCCT GGACAGGGCG TTTCTACTCT 
CTGCTGGATC CCTCTTATGC TAAGAATAAC ATTCCCATTA TTGCATCTGT TTCTGAGCAC 
CAGCCCACAA CCTGGTCTTC CTACTATTTT GATCTACAGC TCCTTGTCTT CATGTTTCCA 
GTTGGCCTCT ATTACTGCTT TAGCAACCTG TCTGATGCTC GGATTTTTAT CATCATGTAT 
GGTGTGACCA GCATGTACTT TTCAGCTGTA ATGGTGCGTC TAATGCTGGT ATTGGCACCT 
GTTATGTGCA TTCTTTCTGG CATTGGTGTT TCCCAGGTGC TGTCCACATA TATGAAAAAT 
CTGGACATAA GTCGCCCAGA CAAGAAGAGC AAGAAGCAAC AGGATTCTAC TTACCCTATT 
AAGAATGAGG TGGCGAGTGG GATGATACTG GTCATGGCTT TCTTTCTCAT CACCTACACG 
TTTCATTCGA CTTGGGTGAC CAGTGAAGCC TATTCTTCTC CCTCCATTGT TCTGTCTGCT 
CGTGGTGGGG ATGGCAGTAG GATCATTTTT GATGACTTCC GAGAAGCGTA TTATTGGCTC 
CGTCACAATA CTCCAGAGGA TGCAAAAGTC ATGTCATGGT GGGATTATGG CTACCAAATT 
ACTGCAATGG CAAATCGGAC AATTTTAGTG GACAATAACA CATGGAATAA TACCCATATT 
TCTCGAGTAG GGCAGGCAAT GGCATCCACA GAAGAAAAAG CCTATGAAAT CATGAGGGAG 
CTTGATGTCA GCTATGTGCT TGTCATTTTT GGAGGCCTTA CTGGGTATTC TTCGGATGAT 
ATCAACAAGT TTCTTTGGAT GGTCCGGATT GGAGGAAGCA CAGAGACAGG AAGACACATT 
AAGGAGAATG ACTACTATAC TCCTACTGGG GAATTCCGTG TTGATCGTGA GGGTTCTCCG 
GTGCTGCTCA ACTGCCTTAT GTACAAAATG TGTTACTACC GCTTTGGGCA GGTCTACACA 
GAAGCCAAGC GTCCACCAGG CTTTGACCGT GTTCGAAATG CTGAGATTGG TAATAAAGAC 
TTTGAGCTTG ATGTCCTGGA GGAAGCGTAT ACCACAGAAC ACTGGCTAGT CAGGATATAC 
AAGGTAAAGG ACCTGGATAA TCGAGGCTTG TCAAGGACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Stt3a ( XM_030244074.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030099934.1 Mus musculus STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) (Stt3a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu42022
Clone ID Related Accession (Same CDS sequence)	XM_030244074.1 , XM_006510038.3
Accession Version	XM_030244074.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGAGCG GGCAGGGAGG CGGGCCTTGG CTGCTGTCAG GGTTGAGTGC GCCGCTGAAC 
GGATGGCAGG GGGAGCAGAG TGGGTTCCTG AGGAGCATCC GTGAGGTATT TGAATATCAT 
CAGTTGCCAC CCATTGATGT CAAGATGACT AAGCTTGGAT TTTTGCGATT GTCCTATGAG 
AAGCAGGACA CACTTCTAAA GCTTCTCATC CTGTCGATGG CTGCTGTGTT ATCTTTTTCT 
ACTCGTCTTT TTGCTGTGCT GAGATTTGAA AGTGTCATCC ATGAGTTTGA TCCGTACTTT 
AATTATCGGA CTACCCGGTT TCTGGCTGAG GAGGGGTTTT ATAAATTCCA TAACTGGTTT 
GATGACCGGG CTTGGTACCC TTTGGGCCGA ATCATTGGAG GAACAATTTA CCCAGGTTTA 
ATGATCACTT CTGCTGCAAT CTACCATGTA CTCCATTTCT TCCATATCAC CATTGACATT 
CGGAATGTCT GTGTTTTCCT GGCCCCGCTT TTCTCCTCTT TCACCACCAT CGTTACGTAC 
CACCTTACCA AAGAGCTCAA GGATGCAGGA GCTGGGCTTC TTGCTGCTGC CATGATTGCT 
GTAGTTCCTG GGTATATTTC TCGATCTGTA GCTGGCTCCT ATGATAATGA AGGAATTGCT 
ATCTTTTGCA TGCTGCTTAC TTACTACATG TGGATCAAGG CAGTGAAGAC TGGTTCCATC 
TATTGGGCTG CCAAGTGTGC CCTCGCTTAT TTCTACATGG TCTCTTCATG GGGAGGCTAT 
GTGTTCCTGA TCAACTTGAT TCCTCTACAT GTCCTGGTGC TAATGCTGAC AGGCCGTTTT 
TCTCACCGGA TCTACGTAGC CTACTGTACT GTTTACTGCC TGGGCACCAT TCTTTCTATG 
CAGATTTCCT TTGTTGGTTT CCAGCCCGTC CTTTCATCAG AACACATGGC AGCCTTTGGA 
GTGTTTGGTC TCTGTCAGAT CCATGCTTTC GTAGATTACC TGCGCAGCAA GTTGAATCCA 
CAGCAATTCG AAGTTCTTTT CCGGAGTGTT ATCTCCCTGG TTGGCTTTGT CCTCCTCACT 
GTGGGAGCTC TCCTCATGCT AACAGGAAAA ATTTCTCCCT GGACAGGGCG TTTCTACTCT 
CTGCTGGATC CCTCTTATGC TAAGAATAAC ATTCCCATTA TTGCATCTGT TTCTGAGCAC 
CAGCCCACAA CCTGGTCTTC CTACTATTTT GATCTACAGC TCCTTGTCTT CATGTTTCCA 
GTTGGCCTCT ATTACTGCTT TAGCAACCTG TCTGATGCTC GGATTTTTAT CATCATGTAT 
GGTGTGACCA GCATGTACTT TTCAGCTGTA ATGGTGCGTC TAATGCTGGT ATTGGCACCT 
GTTATGTGCA TTCTTTCTGG CATTGGTGTT TCCCAGGTGC TGTCCACATA TATGAAAAAT 
CTGGACATAA GTCGCCCAGA CAAGAAGAGC AAGAAGCAAC AGGATTCTAC TTACCCTATT 
AAGAATGAGG TGGCGAGTGG GATGATACTG GTCATGGCTT TCTTTCTCAT CACCTACACG 
TTTCATTCGA CTTGGGTGAC CAGTGAAGCC TATTCTTCTC CCTCCATTGT TCTGTCTGCT 
CGTGGTGGGG ATGGCAGTAG GATCATTTTT GATGACTTCC GAGAAGCGTA TTATTGGCTC 
CGTCACAATA CTCCAGAGGA TGCAAAAGTC ATGTCATGGT GGGATTATGG CTACCAAATT 
ACTGCAATGG CAAATCGGAC AATTTTAGTG GACAATAACA CATGGAATAA TACCCATATT 
TCTCGAGTAG GGCAGGCAAT GGCATCCACA GAAGAAAAAG CCTATGAAAT CATGAGGGAG 
CTTGATGTCA GCTATGTGCT TGTCATTTTT GGAGGCCTTA CTGGGTATTC TTCGGATGAT 
ATCAACAAGT TTCTTTGGAT GGTCCGGATT GGAGGAAGCA CAGAGACAGG AAGACACATT 
AAGGAGAATG ACTACTATAC TCCTACTGGG GAATTCCGTG TTGATCGTGA GGGTTCTCCG 
GTGCTGCTCA ACTGCCTTAT GTACAAAATG TGTTACTACC GCTTTGGGCA GGTCTACACA 
GAAGCCAAGC GTCCACCAGG CTTTGACCGT GTTCGAAATG CTGAGATTGG TAATAAAGAC 
TTTGAGCTTG ATGTCCTGGA GGAAGCGTAT ACCACAGAAC ACTGGCTAGT CAGGATATAC 
AAGGTAAAGG ACCTGGATAA TCGAGGCTTG TCAAGGACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030099934.1
CDS	45..2306
Translation	MRSGQGGGPWLLSGLSAPLNGWQGEQSGFLRSIREVFEYHQLPPIDVKMTKLGFLRLSYEKQDTLLKLLILSMAAVLSFSTRLFAVLRFESVIHEFDPYFNYRTTRFLAEEGFYKFHNWFDDRAWYPLGRIIGGTIYPGLMITSAAIYHVLHFFHITIDIRNVCVFLAPLFSSFTTIVTYHLTKELKDAGAGLLAAAMIAVVPGYISRSVAGSYDNEGIAIFCMLLTYYMWIKAVKTGSIYWAAKCALAYFYMVSSWGGYVFLINLIPLHVLVLMLTGRFSHRIYVAYCTVYCLGTILSMQISFVGFQPVLSSEHMAAFGVFGLCQIHAFVDYLRSKLNPQQFEVLFRSVISLVGFVLLTVGALLMLTGKISPWTGRFYSLLDPSYAKNNIPIIASVSEHQPTTWSSYYFDLQLLVFMFPVGLYYCFSNLSDARIFIIMYGVTSMYFSAVMVRLMLVLAPVMCILSGIGVSQVLSTYMKNLDISRPDKKSKKQQDSTYPIKNEVASGMILVMAFFLITYTFHSTWVTSEAYSSPSIVLSARGGDGSRIIFDDFREAYYWLRHNTPEDAKVMSWWDYGYQITAMANRTILVDNNTWNNTHISRVGQAMASTEEKAYEIMRELDVSYVLVIFGGLTGYSSDDINKFLWMVRIGGSTETGRHIKENDYYTPTGEFRVDREGSPVLLNCLMYKMCYYRFGQVYTEAKRPPGFDRVRNAEIGNKDFELDVLEEAYTTEHWLVRIYKVKDLDNRGLSRT

Target ORF information:

RefSeq Version	XM_030244074.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) (Stt3a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030244074.1

ORF Insert Sequence:

ATGCGGAGCG GGCAGGGAGG CGGGCCTTGG CTGCTGTCAG GGTTGAGTGC GCCGCTGAAC 
GGATGGCAGG GGGAGCAGAG TGGGTTCCTG AGGAGCATCC GTGAGGTATT TGAATATCAT 
CAGTTGCCAC CCATTGATGT CAAGATGACT AAGCTTGGAT TTTTGCGATT GTCCTATGAG 
AAGCAGGACA CACTTCTAAA GCTTCTCATC CTGTCGATGG CTGCTGTGTT ATCTTTTTCT 
ACTCGTCTTT TTGCTGTGCT GAGATTTGAA AGTGTCATCC ATGAGTTTGA TCCGTACTTT 
AATTATCGGA CTACCCGGTT TCTGGCTGAG GAGGGGTTTT ATAAATTCCA TAACTGGTTT 
GATGACCGGG CTTGGTACCC TTTGGGCCGA ATCATTGGAG GAACAATTTA CCCAGGTTTA 
ATGATCACTT CTGCTGCAAT CTACCATGTA CTCCATTTCT TCCATATCAC CATTGACATT 
CGGAATGTCT GTGTTTTCCT GGCCCCGCTT TTCTCCTCTT TCACCACCAT CGTTACGTAC 
CACCTTACCA AAGAGCTCAA GGATGCAGGA GCTGGGCTTC TTGCTGCTGC CATGATTGCT 
GTAGTTCCTG GGTATATTTC TCGATCTGTA GCTGGCTCCT ATGATAATGA AGGAATTGCT 
ATCTTTTGCA TGCTGCTTAC TTACTACATG TGGATCAAGG CAGTGAAGAC TGGTTCCATC 
TATTGGGCTG CCAAGTGTGC CCTCGCTTAT TTCTACATGG TCTCTTCATG GGGAGGCTAT 
GTGTTCCTGA TCAACTTGAT TCCTCTACAT GTCCTGGTGC TAATGCTGAC AGGCCGTTTT 
TCTCACCGGA TCTACGTAGC CTACTGTACT GTTTACTGCC TGGGCACCAT TCTTTCTATG 
CAGATTTCCT TTGTTGGTTT CCAGCCCGTC CTTTCATCAG AACACATGGC AGCCTTTGGA 
GTGTTTGGTC TCTGTCAGAT CCATGCTTTC GTAGATTACC TGCGCAGCAA GTTGAATCCA 
CAGCAATTCG AAGTTCTTTT CCGGAGTGTT ATCTCCCTGG TTGGCTTTGT CCTCCTCACT 
GTGGGAGCTC TCCTCATGCT AACAGGAAAA ATTTCTCCCT GGACAGGGCG TTTCTACTCT 
CTGCTGGATC CCTCTTATGC TAAGAATAAC ATTCCCATTA TTGCATCTGT TTCTGAGCAC 
CAGCCCACAA CCTGGTCTTC CTACTATTTT GATCTACAGC TCCTTGTCTT CATGTTTCCA 
GTTGGCCTCT ATTACTGCTT TAGCAACCTG TCTGATGCTC GGATTTTTAT CATCATGTAT 
GGTGTGACCA GCATGTACTT TTCAGCTGTA ATGGTGCGTC TAATGCTGGT ATTGGCACCT 
GTTATGTGCA TTCTTTCTGG CATTGGTGTT TCCCAGGTGC TGTCCACATA TATGAAAAAT 
CTGGACATAA GTCGCCCAGA CAAGAAGAGC AAGAAGCAAC AGGATTCTAC TTACCCTATT 
AAGAATGAGG TGGCGAGTGG GATGATACTG GTCATGGCTT TCTTTCTCAT CACCTACACG 
TTTCATTCGA CTTGGGTGAC CAGTGAAGCC TATTCTTCTC CCTCCATTGT TCTGTCTGCT 
CGTGGTGGGG ATGGCAGTAG GATCATTTTT GATGACTTCC GAGAAGCGTA TTATTGGCTC 
CGTCACAATA CTCCAGAGGA TGCAAAAGTC ATGTCATGGT GGGATTATGG CTACCAAATT 
ACTGCAATGG CAAATCGGAC AATTTTAGTG GACAATAACA CATGGAATAA TACCCATATT 
TCTCGAGTAG GGCAGGCAAT GGCATCCACA GAAGAAAAAG CCTATGAAAT CATGAGGGAG 
CTTGATGTCA GCTATGTGCT TGTCATTTTT GGAGGCCTTA CTGGGTATTC TTCGGATGAT 
ATCAACAAGT TTCTTTGGAT GGTCCGGATT GGAGGAAGCA CAGAGACAGG AAGACACATT 
AAGGAGAATG ACTACTATAC TCCTACTGGG GAATTCCGTG TTGATCGTGA GGGTTCTCCG 
GTGCTGCTCA ACTGCCTTAT GTACAAAATG TGTTACTACC GCTTTGGGCA GGTCTACACA 
GAAGCCAAGC GTCCACCAGG CTTTGACCGT GTTCGAAATG CTGAGATTGG TAATAAAGAC 
TTTGAGCTTG ATGTCCTGGA GGAAGCGTAT ACCACAGAAC ACTGGCTAGT CAGGATATAC 
AAGGTAAAGG ACCTGGATAA TCGAGGCTTG TCAAGGACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Refined topology model of the STT3/Stt3 protein subunit of the oligosaccharyltransferase complex.
The Journal of biological chemistry292(27)11349-11360(2017 07)
Lara P,?jemalm K,Reithinger J,Holgado A,Maojun Y,Hammed A,Mattle D,Kim H,Nilsson I

Widespread and dynamic translational control of red blood cell development.
Blood129(5)619-629(2017 02)
Alvarez-Dominguez JR,Zhang X,Hu W

Microarray analysis of Foxa2 mutant mouse embryos reveals novel gene expression and inductive roles for the gastrula organizer and its derivatives.
BMC genomics9511(2008 Oct)
Tamplin OJ,Kinzel D,Cox BJ,Bell CE,Rossant J,Lickert H

Proteomic analysis of mammalian oligosaccharyltransferase reveals multiple subcomplexes that contain Sec61, TRAP, and two potential new subunits.
Biochemistry44(16)5982-92(2005 Apr)
Shibatani T,David LL,McCormack AL,Frueh K,Skach WR