Lgr5 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lgr5
Entrez Gene ID	14160
Full Name	leucine rich repeat containing G protein coupled receptor 5
Synonyms	FEX,Gpr49
General protein information	Preferred Names leucine rich repeat containing G protein coupled receptor 5 Names leucine-rich repeat-containing G-protein coupled receptor 5 G protein-coupled receptor 49 follicle expressed hormone orphan G-protein coupled receptor FEX
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10\|10 D2
Summary	The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. [provided by RefSeq, Sep 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_010195.2	NP_034325.2	leucine-rich repeat-containing G-protein coupled receptor 5 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	LGR5	XP_003313909.1
	LGR5	XP_001117502.1
Drosophila melanogaster (fruit fly)	rk	NP_476702.1
Mus musculus (house mouse)	Lgr5	NP_034325.2
Homo sapiens (human)	LGR5	NP_003658.1
Gallus gallus (chicken)	LGR5	XP_425441.2
Rattus norvegicus (Norway rat)	Lgr5	NP_001100254.1
Bos taurus (cattle)	LGR5	NP_001179449.1
Anopheles gambiae (African malaria mosquito)	GPRRK	XP_317111.1
Xenopus tropicalis (tropical clawed frog)	lgr5	XP_002940219.2

Gene Ontology

The following Lgr5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lgr5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu22209	NM_010195.2 Latest version!	Mus musculus leucine rich repeat containing G protein coupled receptor 5 (Lgr5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lgr5 ( NM_010195.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034325.2 Mus musculus leucine rich repeat containing G protein coupled receptor 5 (Lgr5), mRNA.

Price & Availability↑

CloneID	OMu22209
Clone ID Related Accession (Same CDS sequence)	NM_010195.2
Accession Version	NM_010195.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2724bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-26
Organism	Mus musculus(house mouse)
Product	leucine-rich repeat-containing G-protein coupled receptor 5 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK132387.1 and BM935530.1. On Jul 22, 2006 this sequence version replaced NM_010195.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK132387.1, AF110818.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACACCT CCTGCGTCCA CATGCTCCTG TCCTTGCTGG CGCTGCTGCA GTTGGTGGCC 
GCCGGCAGCT CACCGGGACC AGATGCGATA CCGCGGGGCT GCCCATCACA CTGTCACTGT 
GAGCTGGATG GCAGGATGCT GCTCAGGGTG GACTGCTCGG ACCTGGGGCT CTCGGAGCTG 
CCCTCCAACC TCAGCGTCTT CACCTCCTAC CTGGACCTCA GTATGAACAA CATCAGTCAG 
CTACCCGCCA GTCTCCTACA TCGCCTCTGC TTCCTAGAAG AGTTACGTCT TGCTGGAAAT 
GCTTTGACAC ACATTCCCAA GGGAGCGTTC ACGGGCCTTC ACAGCCTCAA AGTGCTTATG 
CTGCAGAACA ACCAGCTGAG ACAGGTTCCG GAGGAAGCGC TACAGAATTT GAGAAGCCTT 
CAATCCCTGC GCCTAGATGC CAACCACATC AGCTACGTGC CACCCAGCTG TTTCAGCGGC 
CTGCACTCCC TGAGGCACCT GTGGCTAGAT GACAATGCTC TCACAGACGT CCCTGTCCAG 
GCTTTCAGAA GTTTATCAGC CCTGCAAGCC ATGACCTTGG CCCTGAACAA AATACACCAC 
ATAGCAGACT ACGCCTTTGG AAACCTCTCC AGCCTCGTGG TTCTGCATCT CCATAATAAT 
AGAATCCACT CCCTGGGAAA GAAATGCTTT GATGGACTCC ACAGCCTGGA GACTTTAGAT 
TTAAATTATA ATAACCTTGA TGAATTCCCC ACTGCAATCA AGACACTCTC CAACCTTAAG 
GAACTAGGAT TCCACAGCAA CAACATCAGG TCAATACCGG AGCGAGCGTT CGTAGGCAAC 
CCTTCTCTTA TCACAATACA CTTCTATGAC AACCCCATCC AATTTGTTGG AGTATCTGCT 
TTTCAGCATT TGCCTGAACT AAGAACACTG ACTTTGAATG GTGCCTCGCA CATTACTGAA 
TTTCCTCACT TGACAGGAAC TGCCACCCTG GAGAGTCTGA CTTTAACTGG AGCAAAGATC 
TCATCTCTTC CCCAGGCCGT CTGTGATCAG TTACCTAATC TCCAAGTGCT AGATTTGTCT 
TACAACCTAC TCGAAGACTT ACCCAGTTTG TCAGGCTGCC AAAAACTTCA GAAAATTGAC 
CTGAGGCATA ACGAGATCTA TGAAATTAAG GGCAGCACTT TTCAGCAGTT GTTTAACCTC 
CGATCTCTGA ACTTAGCATG GAATAAAATT GCTATCATTC ACCCCAATGC GTTTTCTACG 
TTGCCGTCTC TAATAAAGTT GGACCTATCA TCCAATCTCC TGTCGTCCTT CCCTGTGACT 
GGGTTACATG GTTTAACTCA CTTAAAATTA ACAGGGAACC GAGCCTTACA GAGCCTGATA 
CCATCTGCAA ACTTCCCAGA GCTCAAGATT ATAGAAATGC CATCTGCTTA CCAGTGTTGT 
GCATTTGGGG GGTGTGAGAA TGTCTATAAA ATTTCTAACC AATGGAATAA AGACGACGGC 
AACAGTGTGG ACGACCTTCA TAAGAAAGAC GCTGGGTTAT TTCAAGTTCA AGATGAGCGG 
GACCTTGAAG ATTTCCTACT TGACTTTGAG GAAGACCTGA AGGCCCTTCA CTCGGTGCAG 
TGCTCGCCTT CCCCAGGTCC CTTCAAGCCC TGTGAGCACC TATTTGGTAG CTGGCTGATC 
CGAATCGGGG TGTGGACCAC GGCAGTACTG GCGCTTTCCT GCAATGCCTT GGTGGCTTTG 
ACCGTGTTCA GAACTCCTCT GTACATCTCT TCCATAAAGC TGCTAATTGG GGTAATCGCG 
GTAGTGGACA TTCTCATGGG GGTCTCCAGT GCTGTGCTGG CTGCCGTGGA TGCATTCACT 
TTTGGCCGTT TTGCTCAGCA CGGTGCGTGG TGGGAAGACG GAATCGGCTG CCAAATCGTT 
GGCTTCCTGT CCATTTTTGC TTCCGAATCG TCGATCTTCC TGCTCACTCT GGCAGCGCTG 
GAACGAGGTT TTTCTGTCAA GTGCTCTTCG AAGTTTGAAG TGAAAGCTCC CCTTTTTAGC 
CTGAGAGCGA TCGTTTTGCT ATGTGTCCTG TTGGCCCTGA CCATTGCCAC AATCCCCTTG 
CTAGGAGGCA GTAAGTACAA TGCCTCTCCC CTCTGCCTGC CCTTGCCCTT TGGGGAGCCC 
AGCACCACGG GCTACATGGT GGCTCTCGTG TTGCTCAACT CTCTCTGTTT CCTCATAATG 
ACCATTGCCT ACACAAAGCT CTACTGCAGT TTGGAGAAAG GAGAGCTGGA GAATCTTTGG 
GATTGTTCGA TGGTGAAGCA CATTGCTCTG TTGCTCTTCG CCAACTGCAT CCTTTACTGC 
CCCGTGGCTT TCTTATCCTT CTCCTCTTTG CTAAACCTCA CCTTTATCAG TCCTGACGTC 
ATTAAATTTA TACTTCTCGT GATCGTCCCA CTTCCTTCCT GTCTCAACCC ACTTCTCTAC 
ATTGTCTTCA ATCCCCATTT TAAGGAGGAT ATGGGCAGCC TGGGAAAGCA TACCCGTTTC 
TGGATGAGAT CAAAACACGC GAGTCTGCTG TCCATTAACT CGGACGATGT TGAGAAACGG 
TCCTGTGAGT CAACCCAAGC CTTAGTATCC TTTACCCACG CCAGCATAGC CTATGACTTG 
CCTTCCACTT CCGGGGCATC ACCAGCTTAC CCCATGACTG AAAGCTGTCA TCTCTCTTCA 
GTTGCATTTG TCCCATGTCT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034325.2
CDS	288..3011
Translation	MDTSCVHMLLSLLALLQLVAAGSSPGPDAIPRGCPSHCHCELDGRMLLRVDCSDLGLSELPSNLSVFTSYLDLSMNNISQLPASLLHRLCFLEELRLAGNALTHIPKGAFTGLHSLKVLMLQNNQLRQVPEEALQNLRSLQSLRLDANHISYVPPSCFSGLHSLRHLWLDDNALTDVPVQAFRSLSALQAMTLALNKIHHIADYAFGNLSSLVVLHLHNNRIHSLGKKCFDGLHSLETLDLNYNNLDEFPTAIKTLSNLKELGFHSNNIRSIPERAFVGNPSLITIHFYDNPIQFVGVSAFQHLPELRTLTLNGASHITEFPHLTGTATLESLTLTGAKISSLPQAVCDQLPNLQVLDLSYNLLEDLPSLSGCQKLQKIDLRHNEIYEIKGSTFQQLFNLRSLNLAWNKIAIIHPNAFSTLPSLIKLDLSSNLLSSFPVTGLHGLTHLKLTGNRALQSLIPSANFPELKIIEMPSAYQCCAFGGCENVYKISNQWNKDDGNSVDDLHKKDAGLFQVQDERDLEDFLLDFEEDLKALHSVQCSPSPGPFKPCEHLFGSWLIRIGVWTTAVLALSCNALVALTVFRTPLYISSIKLLIGVIAVVDILMGVSSAVLAAVDAFTFGRFAQHGAWWEDGIGCQIVGFLSIFASESSIFLLTLAALERGFSVKCSSKFEVKAPLFSLRAIVLLCVLLALTIATIPLLGGSKYNASPLCLPLPFGEPSTTGYMVALVLLNSLCFLIMTIAYTKLYCSLEKGELENLWDCSMVKHIALLLFANCILYCPVAFLSFSSLLNLTFISPDVIKFILLVIVPLPSCLNPLLYIVFNPHFKEDMGSLGKHTRFWMRSKHASLLSINSDDVEKRSCESTQALVSFTHASIAYDLPSTSGASPAYPMTESCHLSSVAFVPCL

Target ORF information:

RefSeq Version	NM_010195.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus leucine rich repeat containing G protein coupled receptor 5 (Lgr5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010195.2

ORF Insert Sequence:

ATGGACACCT CCTGCGTCCA CATGCTCCTG TCCTTGCTGG CGCTGCTGCA GTTGGTGGCC 
GCCGGCAGCT CACCGGGACC AGATGCGATA CCGCGGGGCT GCCCATCACA CTGTCACTGT 
GAGCTGGATG GCAGGATGCT GCTCAGGGTG GACTGCTCGG ACCTGGGGCT CTCGGAGCTG 
CCCTCCAACC TCAGCGTCTT CACCTCCTAC CTGGACCTCA GTATGAACAA CATCAGTCAG 
CTACCCGCCA GTCTCCTACA TCGCCTCTGC TTCCTAGAAG AGTTACGTCT TGCTGGAAAT 
GCTTTGACAC ACATTCCCAA GGGAGCGTTC ACGGGCCTTC ACAGCCTCAA AGTGCTTATG 
CTGCAGAACA ACCAGCTGAG ACAGGTTCCG GAGGAAGCGC TACAGAATTT GAGAAGCCTT 
CAATCCCTGC GCCTAGATGC CAACCACATC AGCTACGTGC CACCCAGCTG TTTCAGCGGC 
CTGCACTCCC TGAGGCACCT GTGGCTAGAT GACAATGCTC TCACAGACGT CCCTGTCCAG 
GCTTTCAGAA GTTTATCAGC CCTGCAAGCC ATGACCTTGG CCCTGAACAA AATACACCAC 
ATAGCAGACT ACGCCTTTGG AAACCTCTCC AGCCTCGTGG TTCTGCATCT CCATAATAAT 
AGAATCCACT CCCTGGGAAA GAAATGCTTT GATGGACTCC ACAGCCTGGA GACTTTAGAT 
TTAAATTATA ATAACCTTGA TGAATTCCCC ACTGCAATCA AGACACTCTC CAACCTTAAG 
GAACTAGGAT TCCACAGCAA CAACATCAGG TCAATACCGG AGCGAGCGTT CGTAGGCAAC 
CCTTCTCTTA TCACAATACA CTTCTATGAC AACCCCATCC AATTTGTTGG AGTATCTGCT 
TTTCAGCATT TGCCTGAACT AAGAACACTG ACTTTGAATG GTGCCTCGCA CATTACTGAA 
TTTCCTCACT TGACAGGAAC TGCCACCCTG GAGAGTCTGA CTTTAACTGG AGCAAAGATC 
TCATCTCTTC CCCAGGCCGT CTGTGATCAG TTACCTAATC TCCAAGTGCT AGATTTGTCT 
TACAACCTAC TCGAAGACTT ACCCAGTTTG TCAGGCTGCC AAAAACTTCA GAAAATTGAC 
CTGAGGCATA ACGAGATCTA TGAAATTAAG GGCAGCACTT TTCAGCAGTT GTTTAACCTC 
CGATCTCTGA ACTTAGCATG GAATAAAATT GCTATCATTC ACCCCAATGC GTTTTCTACG 
TTGCCGTCTC TAATAAAGTT GGACCTATCA TCCAATCTCC TGTCGTCCTT CCCTGTGACT 
GGGTTACATG GTTTAACTCA CTTAAAATTA ACAGGGAACC GAGCCTTACA GAGCCTGATA 
CCATCTGCAA ACTTCCCAGA GCTCAAGATT ATAGAAATGC CATCTGCTTA CCAGTGTTGT 
GCATTTGGGG GGTGTGAGAA TGTCTATAAA ATTTCTAACC AATGGAATAA AGACGACGGC 
AACAGTGTGG ACGACCTTCA TAAGAAAGAC GCTGGGTTAT TTCAAGTTCA AGATGAGCGG 
GACCTTGAAG ATTTCCTACT TGACTTTGAG GAAGACCTGA AGGCCCTTCA CTCGGTGCAG 
TGCTCGCCTT CCCCAGGTCC CTTCAAGCCC TGTGAGCACC TATTTGGTAG CTGGCTGATC 
CGAATCGGGG TGTGGACCAC GGCAGTACTG GCGCTTTCCT GCAATGCCTT GGTGGCTTTG 
ACCGTGTTCA GAACTCCTCT GTACATCTCT TCCATAAAGC TGCTAATTGG GGTAATCGCG 
GTAGTGGACA TTCTCATGGG GGTCTCCAGT GCTGTGCTGG CTGCCGTGGA TGCATTCACT 
TTTGGCCGTT TTGCTCAGCA CGGTGCGTGG TGGGAAGACG GAATCGGCTG CCAAATCGTT 
GGCTTCCTGT CCATTTTTGC TTCCGAATCG TCGATCTTCC TGCTCACTCT GGCAGCGCTG 
GAACGAGGTT TTTCTGTCAA GTGCTCTTCG AAGTTTGAAG TGAAAGCTCC CCTTTTTAGC 
CTGAGAGCGA TCGTTTTGCT ATGTGTCCTG TTGGCCCTGA CCATTGCCAC AATCCCCTTG 
CTAGGAGGCA GTAAGTACAA TGCCTCTCCC CTCTGCCTGC CCTTGCCCTT TGGGGAGCCC 
AGCACCACGG GCTACATGGT GGCTCTCGTG TTGCTCAACT CTCTCTGTTT CCTCATAATG 
ACCATTGCCT ACACAAAGCT CTACTGCAGT TTGGAGAAAG GAGAGCTGGA GAATCTTTGG 
GATTGTTCGA TGGTGAAGCA CATTGCTCTG TTGCTCTTCG CCAACTGCAT CCTTTACTGC 
CCCGTGGCTT TCTTATCCTT CTCCTCTTTG CTAAACCTCA CCTTTATCAG TCCTGACGTC 
ATTAAATTTA TACTTCTCGT GATCGTCCCA CTTCCTTCCT GTCTCAACCC ACTTCTCTAC 
ATTGTCTTCA ATCCCCATTT TAAGGAGGAT ATGGGCAGCC TGGGAAAGCA TACCCGTTTC 
TGGATGAGAT CAAAACACGC GAGTCTGCTG TCCATTAACT CGGACGATGT TGAGAAACGG 
TCCTGTGAGT CAACCCAAGC CTTAGTATCC TTTACCCACG CCAGCATAGC CTATGACTTG 
CCTTCCACTT CCGGGGCATC ACCAGCTTAC CCCATGACTG AAAGCTGTCA TCTCTCTTCA 
GTTGCATTTG TCCCATGTCT CTAG