Ephb4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ephb4
Entrez Gene ID	13846
Full Name	Eph receptor B4
Synonyms	AI042935,Htk,MDK2,Myk1,Tyro11,b2b2412Clo
General protein information	Preferred Names Eph receptor B4 Names ephrin type-B receptor 4 developmental kinase 2 hepatoma transmembrane kinase mDK-2 tyrosine kinase MYK-1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 G2

mRNA and Protein(s)

mRNA	Protein	Name
NM_010144.6	NP_034274.4	ephrin type-B receptor 4 isoform b precursor
NM_001159571.1	NP_001153043.1	ephrin type-B receptor 4 isoform a precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	EPHB4	NP_004435.3
Canis lupus familiaris (dog)	EPHB4	XP_546948.3
Mus musculus (house mouse)	Ephb4	NP_001153043.1
Rattus norvegicus (Norway rat)	Ephb4	XP_003751205.1
	EPHB4	XP_002803271.1
Bos taurus (cattle)	EPHB4	NP_001193197.1
Danio rerio (zebrafish)	ephb4b	NP_571492.1
Pan troglodytes (chimpanzee)	EPHB4	XP_519269.3
Danio rerio (zebrafish)	ephb4a	NP_571489.1

Gene Ontology

The following Ephb4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ephb4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu22519	NM_001159571.1 Latest version!	Mus musculus Eph receptor B4 (Ephb4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu22536	NM_010144.6 Latest version!	Mus musculus Eph receptor B4 (Ephb4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ephb4 ( NM_001159571.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001153043.1 Mus musculus Eph receptor B4 (Ephb4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu22519
Clone ID Related Accession (Same CDS sequence)	NM_001159571.1
Accession Version	NM_001159571.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2991bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Mus musculus(house mouse)
Product	ephrin type-B receptor 4 isoform a precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK049340.1, AK046884.1 and BB677774.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK046884.1, BC013342.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCTCC GAGCGCTGCT GTGCTGGGCT TCCCTCGCCA CTGCTTTAGA AGAGACCCTG 
TTGAACACAA AACTGGAAAC GGCGGATCTG AAATGGGTGA CTTACCCTCA GGCAGAGGGC 
CAGTGGGAGG AGCTAAGCGG CCTGGATGAG GAACAGCACA GCGTCCGCAC CTATGAGGTG 
TGCGACATGA AGCGTCCAGG GGGCCAGGCT CACTGGCTGC GCACTGGCTG GGTCCCAAGG 
CGAGGTGCTG TCCACGTGTA TGCCACGATA CGCTTCACCA TGATGGAATG CCTGTCCCTG 
CCGAGGGCCA GTCGCTCCTG CAAGGAGACA TTCACTGTCT TCTATTACGA GAGCGAAGCT 
GATACGGCCA CGGCCCATAC GCCCGCCTGG ATGGAGAACC CCTACATCAA GGTGGACACA 
GTGGCCGCAG AACATCTGAC TCGGAAGCGC CCTGGAGCTG AAGCCACAGG GAAAGTTAAT 
ATCAAGACGC TGCGCCTGGG TCCTCTCAGC AAAGCTGGCT TCTACCTGGC TTTCCAGGAC 
CAAGGAGCCT GCATGGCTCT GCTCTCCCTG CATCTCTTTT ACAAGAAGTG CTCCTGGCTG 
ATCACGAACT TGACCTACTT CCCCGAGACG GTGCCTCGGG AGCTCGTGGT GCCGGTGGCA 
GGTAGCTGCG TGGCCAACGC GGTCCCTACC GCCAACCCCA GCCCCAGCCT CTACTGCCGG 
GAAGATGGTC AATGGGCTGA GCAGCAGGTC ACGGGCTGCA GCTGCGCGCC AGGGTACGAG 
GCTGCGGAAA GCAACAAAGT ATGCAGAGCC TGTGGCCAGG GAACCTTCAA GCCCCAAATA 
GGAGACGAGT CCTGCCTGCC ATGCCCAGCC AACAGCCACT CGAATAACAT TGGGTCTCCT 
GTCTGCCTGT GTCGAATTGG GTATTACCGG GCCCGCTCAG ACCCCCGGAG TTCACCTTGC 
ACTACCCCAC CCTCTGCTCC AAGAAGCGTG GTTCACCATT TGAATGGTTC CACCCTGCGC 
CTGGAATGGA GTGCTCCCCT TGAGTCCGGA GGCCGAGAGG ACCTCACTTA TGCTGTACGC 
TGCCGAGAGT GCCGTCCTGG GGGTTCCTGC TTGCCCTGTG GGGGCGACAT GACCTTCGAC 
CCCGGTCCTC GAGACCTGGT TGAGCCCTGG GTGGCAATCC GAGGGCTGCG TCCTGATGTC 
ACCTATACCT TTGAGGTTGC TGCTTTGAAT GGTGTGTCTA CCTTAGCCAC TGGACCACCT 
CCTTTTGAGC CTGTCAATGT CACCACTGAC CGTGAGGGGC GCATTGGGTT TCTTTCTCCT 
GCAGTGCCTC CTGCAGTGTC TGACATCCGA GTGACTCGGT CGTCACCCAG CAGCTTGATC 
CTGTCATGGG CTATCCCCAG AGCACCCAGT GGGGCCGTGC TGGACTACGA GGTCAAGTAT 
CATGAGAAGG GCGCAGAGGG CCCCAGCAGT GTTCGTTTCC TGAAGACATC AGAAAACCGA 
GCTGAGCTCC GGGGGCTGAA GCGGGGAGCC AGCTATCTGG TCCAGGTACG CGCACGGTCC 
GAGGCTGGCT ACGGTCCCTT CGGCCAGGAG CATCACAGTC AGACTCAACT GGATGAGAGC 
GAGAGCTGGC GGGAGCAGCT GGCCCTGATT GCAGGCACTG CGGTTGTGGG TGTGGTCCTG 
GTCCTGGTGG TCGTCATCAT TGCAGTTCTC TGCCTCAGGA AGCAGAGCAA TGGGAGGGAA 
GTTGAGTACT CGGATAAGCA TGGGCAGTAT CTCATCGGGC ACGGTACCAA GGTCTACATT 
GATCCTTTTA CTTACGAAGA CCCTAATGAG GCAGTGAGGG AATTTGCCAA AGAGATCGAT 
GTCTCCTATG TCAAGATTGA AGAGGTAATT GGTGCAGGTG AGTTCGGCGA GGTGTGCCGG 
GGTCGGCTGA AGGCACCAGG GAAAAAGGAG AGCTGTGTGG CCATCAAGAC TCTGAAGGGT 
GGCTACACCG AGCGCCAGAG GCGTGAGTTC CTGAGCGAGG CCTCCATCAT GGGCCAGTTC 
GAGCATCCCA ACATCATCCG CCTCGAGGGC GTGGTCACCA ACAGTGTGCC GGTTATGATC 
CTCACGGAAT TCATGGAGAA CGGAGCCCTG GACTCCTTCC TGCGGCTGAA CGACGGGCAG 
TTCACAGTCA TCCAGCTGGT GGGCATGCTG AGGGGCATCG CCTCGGGCAT GCGGTACCTG 
GCTGAAATGA GCTATGTCCA CCGAGACCTG GCTGCTCGGA ACATCTTGGT CAACAGTAAC 
CTGGTCTGCA AGGTGTCCGA CTTTGGCCTC TCCAGATTCT TGGAGGAGAA CTCCTCTGAT 
CCCACCTACA CAAGTTCCCT GGGAGGCAAG ATTCCCATCC GATGGACCGC CCCTGAAGCC 
ATTGCCTTCA GGAAGTTCAC CTCTGCCAGT GATGCCTGGA GCTATGGGAT CGTCATGTGG 
GAGGTCATGT CTTTTGGGGA ACGGCCATAC TGGGACATGA GCAACCAGGA TGTGATCAAT 
GCCATTGAAC AGGACTACCG GCTGCCTCCT CCTCCAGACT GCCCCACCTC CCTCCACCAG 
CTCATGCTGG ATTGTTGGCA GAAGGACCGG AATGCCCGGC CCCGCTTTCC CCAGGTGGTC 
AGCGCTCTGG ACAAGATGAT CCGGAATCCC GCTAGCCTCA AAATCGTGGC CAGGGAGAAT 
GGCGGGGCCT CACATCCACT CTTGGACCAA CGGCAGCCTC ACTATTCTGC TTTCGGTTCT 
GTGGGCGAGT GGCTTCGAGC CATCAAGATG GGAAGATACG AGGAAAGTTT TGCAGCGGCT 
GGATTCGGCT CCTTTGAGGT GGTCAGTCAG ATCTCTGCCG AGGACCTTCT CCGAATTGGA 
GTCACTCTGG CAGGACACCA GAAGAAAATC TTGGCCAGTG TGCAGCATAT GAAGTCCCAA 
GCTAAGCCAG GAGCCCCTGG TGGGACAGGG GGACCAGCCC AGCAGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153043.1
CDS	490..3480
Translation	MELRALLCWASLATALEETLLNTKLETADLKWVTYPQAEGQWEELSGLDEEQHSVRTYEVCDMKRPGGQAHWLRTGWVPRRGAVHVYATIRFTMMECLSLPRASRSCKETFTVFYYESEADTATAHTPAWMENPYIKVDTVAAEHLTRKRPGAEATGKVNIKTLRLGPLSKAGFYLAFQDQGACMALLSLHLFYKKCSWLITNLTYFPETVPRELVVPVAGSCVANAVPTANPSPSLYCREDGQWAEQQVTGCSCAPGYEAAESNKVCRACGQGTFKPQIGDESCLPCPANSHSNNIGSPVCLCRIGYYRARSDPRSSPCTTPPSAPRSVVHHLNGSTLRLEWSAPLESGGREDLTYAVRCRECRPGGSCLPCGGDMTFDPGPRDLVEPWVAIRGLRPDVTYTFEVAALNGVSTLATGPPPFEPVNVTTDREGRIGFLSPAVPPAVSDIRVTRSSPSSLILSWAIPRAPSGAVLDYEVKYHEKGAEGPSSVRFLKTSENRAELRGLKRGASYLVQVRARSEAGYGPFGQEHHSQTQLDESESWREQLALIAGTAVVGVVLVLVVVIIAVLCLRKQSNGREVEYSDKHGQYLIGHGTKVYIDPFTYEDPNEAVREFAKEIDVSYVKIEEVIGAGEFGEVCRGRLKAPGKKESCVAIKTLKGGYTERQRREFLSEASIMGQFEHPNIIRLEGVVTNSVPVMILTEFMENGALDSFLRLNDGQFTVIQLVGMLRGIASGMRYLAEMSYVHRDLAARNILVNSNLVCKVSDFGLSRFLEENSSDPTYTSSLGGKIPIRWTAPEAIAFRKFTSASDAWSYGIVMWEVMSFGERPYWDMSNQDVINAIEQDYRLPPPPDCPTSLHQLMLDCWQKDRNARPRFPQVVSALDKMIRNPASLKIVARENGGASHPLLDQRQPHYSAFGSVGEWLRAIKMGRYEESFAAAGFGSFEVVSQISAEDLLRIGVTLAGHQKKILASVQHMKSQAKPGAPGGTGGPAQQF

Target ORF information:

RefSeq Version	NM_001159571.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Eph receptor B4 (Ephb4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001159571.1

ORF Insert Sequence:

ATGGAGCTCC GAGCGCTGCT GTGCTGGGCT TCCCTCGCCA CTGCTTTAGA AGAGACCCTG 
TTGAACACAA AACTGGAAAC GGCGGATCTG AAATGGGTGA CTTACCCTCA GGCAGAGGGC 
CAGTGGGAGG AGCTAAGCGG CCTGGATGAG GAACAGCACA GCGTCCGCAC CTATGAGGTG 
TGCGACATGA AGCGTCCAGG GGGCCAGGCT CACTGGCTGC GCACTGGCTG GGTCCCAAGG 
CGAGGTGCTG TCCACGTGTA TGCCACGATA CGCTTCACCA TGATGGAATG CCTGTCCCTG 
CCGAGGGCCA GTCGCTCCTG CAAGGAGACA TTCACTGTCT TCTATTACGA GAGCGAAGCT 
GATACGGCCA CGGCCCATAC GCCCGCCTGG ATGGAGAACC CCTACATCAA GGTGGACACA 
GTGGCCGCAG AACATCTGAC TCGGAAGCGC CCTGGAGCTG AAGCCACAGG GAAAGTTAAT 
ATCAAGACGC TGCGCCTGGG TCCTCTCAGC AAAGCTGGCT TCTACCTGGC TTTCCAGGAC 
CAAGGAGCCT GCATGGCTCT GCTCTCCCTG CATCTCTTTT ACAAGAAGTG CTCCTGGCTG 
ATCACGAACT TGACCTACTT CCCCGAGACG GTGCCTCGGG AGCTCGTGGT GCCGGTGGCA 
GGTAGCTGCG TGGCCAACGC GGTCCCTACC GCCAACCCCA GCCCCAGCCT CTACTGCCGG 
GAAGATGGTC AATGGGCTGA GCAGCAGGTC ACGGGCTGCA GCTGCGCGCC AGGGTACGAG 
GCTGCGGAAA GCAACAAAGT ATGCAGAGCC TGTGGCCAGG GAACCTTCAA GCCCCAAATA 
GGAGACGAGT CCTGCCTGCC ATGCCCAGCC AACAGCCACT CGAATAACAT TGGGTCTCCT 
GTCTGCCTGT GTCGAATTGG GTATTACCGG GCCCGCTCAG ACCCCCGGAG TTCACCTTGC 
ACTACCCCAC CCTCTGCTCC AAGAAGCGTG GTTCACCATT TGAATGGTTC CACCCTGCGC 
CTGGAATGGA GTGCTCCCCT TGAGTCCGGA GGCCGAGAGG ACCTCACTTA TGCTGTACGC 
TGCCGAGAGT GCCGTCCTGG GGGTTCCTGC TTGCCCTGTG GGGGCGACAT GACCTTCGAC 
CCCGGTCCTC GAGACCTGGT TGAGCCCTGG GTGGCAATCC GAGGGCTGCG TCCTGATGTC 
ACCTATACCT TTGAGGTTGC TGCTTTGAAT GGTGTGTCTA CCTTAGCCAC TGGACCACCT 
CCTTTTGAGC CTGTCAATGT CACCACTGAC CGTGAGGGGC GCATTGGGTT TCTTTCTCCT 
GCAGTGCCTC CTGCAGTGTC TGACATCCGA GTGACTCGGT CGTCACCCAG CAGCTTGATC 
CTGTCATGGG CTATCCCCAG AGCACCCAGT GGGGCCGTGC TGGACTACGA GGTCAAGTAT 
CATGAGAAGG GCGCAGAGGG CCCCAGCAGT GTTCGTTTCC TGAAGACATC AGAAAACCGA 
GCTGAGCTCC GGGGGCTGAA GCGGGGAGCC AGCTATCTGG TCCAGGTACG CGCACGGTCC 
GAGGCTGGCT ACGGTCCCTT CGGCCAGGAG CATCACAGTC AGACTCAACT GGATGAGAGC 
GAGAGCTGGC GGGAGCAGCT GGCCCTGATT GCAGGCACTG CGGTTGTGGG TGTGGTCCTG 
GTCCTGGTGG TCGTCATCAT TGCAGTTCTC TGCCTCAGGA AGCAGAGCAA TGGGAGGGAA 
GTTGAGTACT CGGATAAGCA TGGGCAGTAT CTCATCGGGC ACGGTACCAA GGTCTACATT 
GATCCTTTTA CTTACGAAGA CCCTAATGAG GCAGTGAGGG AATTTGCCAA AGAGATCGAT 
GTCTCCTATG TCAAGATTGA AGAGGTAATT GGTGCAGGTG AGTTCGGCGA GGTGTGCCGG 
GGTCGGCTGA AGGCACCAGG GAAAAAGGAG AGCTGTGTGG CCATCAAGAC TCTGAAGGGT 
GGCTACACCG AGCGCCAGAG GCGTGAGTTC CTGAGCGAGG CCTCCATCAT GGGCCAGTTC 
GAGCATCCCA ACATCATCCG CCTCGAGGGC GTGGTCACCA ACAGTGTGCC GGTTATGATC 
CTCACGGAAT TCATGGAGAA CGGAGCCCTG GACTCCTTCC TGCGGCTGAA CGACGGGCAG 
TTCACAGTCA TCCAGCTGGT GGGCATGCTG AGGGGCATCG CCTCGGGCAT GCGGTACCTG 
GCTGAAATGA GCTATGTCCA CCGAGACCTG GCTGCTCGGA ACATCTTGGT CAACAGTAAC 
CTGGTCTGCA AGGTGTCCGA CTTTGGCCTC TCCAGATTCT TGGAGGAGAA CTCCTCTGAT 
CCCACCTACA CAAGTTCCCT GGGAGGCAAG ATTCCCATCC GATGGACCGC CCCTGAAGCC 
ATTGCCTTCA GGAAGTTCAC CTCTGCCAGT GATGCCTGGA GCTATGGGAT CGTCATGTGG 
GAGGTCATGT CTTTTGGGGA ACGGCCATAC TGGGACATGA GCAACCAGGA TGTGATCAAT 
GCCATTGAAC AGGACTACCG GCTGCCTCCT CCTCCAGACT GCCCCACCTC CCTCCACCAG 
CTCATGCTGG ATTGTTGGCA GAAGGACCGG AATGCCCGGC CCCGCTTTCC CCAGGTGGTC 
AGCGCTCTGG ACAAGATGAT CCGGAATCCC GCTAGCCTCA AAATCGTGGC CAGGGAGAAT 
GGCGGGGCCT CACATCCACT CTTGGACCAA CGGCAGCCTC ACTATTCTGC TTTCGGTTCT 
GTGGGCGAGT GGCTTCGAGC CATCAAGATG GGAAGATACG AGGAAAGTTT TGCAGCGGCT 
GGATTCGGCT CCTTTGAGGT GGTCAGTCAG ATCTCTGCCG AGGACCTTCT CCGAATTGGA 
GTCACTCTGG CAGGACACCA GAAGAAAATC TTGGCCAGTG TGCAGCATAT GAAGTCCCAA 
GCTAAGCCAG GAGCCCCTGG TGGGACAGGG GGACCAGCCC AGCAGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ephb4 ( NM_010144.6 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034274.4 Mus musculus Eph receptor B4 (Ephb4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu22536
Clone ID Related Accession (Same CDS sequence)	NM_010144.6
Accession Version	NM_010144.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2964bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	ephrin type-B receptor 4 isoform b precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK132392.1, BC090839.1, AK049340.1 and BB677774.1. On Apr 22, 2009 this sequence version replaced NM_010144.5. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region compared to variant 1. This results in a shorter protein (isoform b) compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK132392.1, AK049340.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCTCC GAGCGCTGCT GTGCTGGGCT TCCCTCGCCA CTGCTTTAGA AGAGACCCTG 
TTGAACACAA AACTGGAAAC GGCGGATCTG AAATGGGTGA CTTACCCTCA GGCAGAGGGC 
CAGTGGGAGG AGCTAAGCGG CCTGGATGAG GAACAGCACA GCGTCCGCAC CTATGAGGTG 
TGCGACATGA AGCGTCCAGG GGGCCAGGCT CACTGGCTGC GCACTGGCTG GGTCCCAAGG 
CGAGGTGCTG TCCACGTGTA TGCCACGATA CGCTTCACCA TGATGGAATG CCTGTCCCTG 
CCGAGGGCCA GTCGCTCCTG CAAGGAGACA TTCACTGTCT TCTATTACGA GAGCGAAGCT 
GATACGGCCA CGGCCCATAC GCCCGCCTGG ATGGAGAACC CCTACATCAA GGTGGACACA 
GTGGCCGCAG AACATCTGAC TCGGAAGCGC CCTGGAGCTG AAGCCACAGG GAAAGTTAAT 
ATCAAGACGC TGCGCCTGGG TCCTCTCAGC AAAGCTGGCT TCTACCTGGC TTTCCAGGAC 
CAAGGAGCCT GCATGGCTCT GCTCTCCCTG CATCTCTTTT ACAAGAAGTG CTCCTGGCTG 
ATCACGAACT TGACCTACTT CCCCGAGACG GTGCCTCGGG AGCTCGTGGT GCCGGTGGCA 
GGTAGCTGCG TGGCCAACGC GGTCCCTACC GCCAACCCCA GCCCCAGCCT CTACTGCCGG 
GAAGATGGTC AATGGGCTGA GCAGCAGGTC ACGGGCTGCA GCTGCGCGCC AGGGTACGAG 
GCTGCGGAAA GCAACAAAGT ATGCAGAGCC TGTGGCCAGG GAACCTTCAA GCCCCAAATA 
GGAGACGAGT CCTGCCTGCC ATGCCCAGCC AACAGCCACT CGAATAACAT TGGGTCTCCT 
GTCTGCCTGT GTCGAATTGG GTATTACCGG GCCCGCTCAG ACCCCCGGAG TTCACCTTGC 
ACTACCCCAC CCTCTGCTCC AAGAAGCGTG GTTCACCATT TGAATGGTTC CACCCTGCGC 
CTGGAATGGA GTGCTCCCCT TGAGTCCGGA GGCCGAGAGG ACCTCACTTA TGCTGTACGC 
TGCCGAGAGT GCCGTCCTGG GGGTTCCTGC TTGCCCTGTG GGGGCGACAT GACCTTCGAC 
CCCGGTCCTC GAGACCTGGT TGAGCCCTGG GTGGCAATCC GAGGGCTGCG TCCTGATGTC 
ACCTATACCT TTGAGGTTGC TGCTTTGAAT GGTGTGTCTA CCTTAGCCAC TGGACCACCT 
CCTTTTGAGC CTGTCAATGT CACCACTGAC CGTGAGGTGC CTCCTGCAGT GTCTGACATC 
CGAGTGACTC GGTCGTCACC CAGCAGCTTG ATCCTGTCAT GGGCTATCCC CAGAGCACCC 
AGTGGGGCCG TGCTGGACTA CGAGGTCAAG TATCATGAGA AGGGCGCAGA GGGCCCCAGC 
AGTGTTCGTT TCCTGAAGAC ATCAGAAAAC CGAGCTGAGC TCCGGGGGCT GAAGCGGGGA 
GCCAGCTATC TGGTCCAGGT ACGCGCACGG TCCGAGGCTG GCTACGGTCC CTTCGGCCAG 
GAGCATCACA GTCAGACTCA ACTGGATGAG AGCGAGAGCT GGCGGGAGCA GCTGGCCCTG 
ATTGCAGGCA CTGCGGTTGT GGGTGTGGTC CTGGTCCTGG TGGTCGTCAT CATTGCAGTT 
CTCTGCCTCA GGAAGCAGAG CAATGGGAGG GAAGTTGAGT ACTCGGATAA GCATGGGCAG 
TATCTCATCG GGCACGGTAC CAAGGTCTAC ATTGATCCTT TTACTTACGA AGACCCTAAT 
GAGGCAGTGA GGGAATTTGC CAAAGAGATC GATGTCTCCT ATGTCAAGAT TGAAGAGGTA 
ATTGGTGCAG GTGAGTTCGG CGAGGTGTGC CGGGGTCGGC TGAAGGCACC AGGGAAAAAG 
GAGAGCTGTG TGGCCATCAA GACTCTGAAG GGTGGCTACA CCGAGCGCCA GAGGCGTGAG 
TTCCTGAGCG AGGCCTCCAT CATGGGCCAG TTCGAGCATC CCAACATCAT CCGCCTCGAG 
GGCGTGGTCA CCAACAGTGT GCCGGTTATG ATCCTCACGG AATTCATGGA GAACGGAGCC 
CTGGACTCCT TCCTGCGGCT GAACGACGGG CAGTTCACAG TCATCCAGCT GGTGGGCATG 
CTGAGGGGCA TCGCCTCGGG CATGCGGTAC CTGGCTGAAA TGAGCTATGT CCACCGAGAC 
CTGGCTGCTC GGAACATCTT GGTCAACAGT AACCTGGTCT GCAAGGTGTC CGACTTTGGC 
CTCTCCAGAT TCTTGGAGGA GAACTCCTCT GATCCCACCT ACACAAGTTC CCTGGGAGGC 
AAGATTCCCA TCCGATGGAC CGCCCCTGAA GCCATTGCCT TCAGGAAGTT CACCTCTGCC 
AGTGATGCCT GGAGCTATGG GATCGTCATG TGGGAGGTCA TGTCTTTTGG GGAACGGCCA 
TACTGGGACA TGAGCAACCA GGATGTGATC AATGCCATTG AACAGGACTA CCGGCTGCCT 
CCTCCTCCAG ACTGCCCCAC CTCCCTCCAC CAGCTCATGC TGGATTGTTG GCAGAAGGAC 
CGGAATGCCC GGCCCCGCTT TCCCCAGGTG GTCAGCGCTC TGGACAAGAT GATCCGGAAT 
CCCGCTAGCC TCAAAATCGT GGCCAGGGAG AATGGCGGGG CCTCACATCC ACTCTTGGAC 
CAACGGCAGC CTCACTATTC TGCTTTCGGT TCTGTGGGCG AGTGGCTTCG AGCCATCAAG 
ATGGGAAGAT ACGAGGAAAG TTTTGCAGCG GCTGGATTCG GCTCCTTTGA GGTGGTCAGT 
CAGATCTCTG CCGAGGACCT TCTCCGAATT GGAGTCACTC TGGCAGGACA CCAGAAGAAA 
ATCTTGGCCA GTGTGCAGCA TATGAAGTCC CAAGCTAAGC CAGGAGCCCC TGGTGGGACA 
GGGGGACCAG CCCAGCAGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034274.4
CDS	490..3453
Translation	MELRALLCWASLATALEETLLNTKLETADLKWVTYPQAEGQWEELSGLDEEQHSVRTYEVCDMKRPGGQAHWLRTGWVPRRGAVHVYATIRFTMMECLSLPRASRSCKETFTVFYYESEADTATAHTPAWMENPYIKVDTVAAEHLTRKRPGAEATGKVNIKTLRLGPLSKAGFYLAFQDQGACMALLSLHLFYKKCSWLITNLTYFPETVPRELVVPVAGSCVANAVPTANPSPSLYCREDGQWAEQQVTGCSCAPGYEAAESNKVCRACGQGTFKPQIGDESCLPCPANSHSNNIGSPVCLCRIGYYRARSDPRSSPCTTPPSAPRSVVHHLNGSTLRLEWSAPLESGGREDLTYAVRCRECRPGGSCLPCGGDMTFDPGPRDLVEPWVAIRGLRPDVTYTFEVAALNGVSTLATGPPPFEPVNVTTDREVPPAVSDIRVTRSSPSSLILSWAIPRAPSGAVLDYEVKYHEKGAEGPSSVRFLKTSENRAELRGLKRGASYLVQVRARSEAGYGPFGQEHHSQTQLDESESWREQLALIAGTAVVGVVLVLVVVIIAVLCLRKQSNGREVEYSDKHGQYLIGHGTKVYIDPFTYEDPNEAVREFAKEIDVSYVKIEEVIGAGEFGEVCRGRLKAPGKKESCVAIKTLKGGYTERQRREFLSEASIMGQFEHPNIIRLEGVVTNSVPVMILTEFMENGALDSFLRLNDGQFTVIQLVGMLRGIASGMRYLAEMSYVHRDLAARNILVNSNLVCKVSDFGLSRFLEENSSDPTYTSSLGGKIPIRWTAPEAIAFRKFTSASDAWSYGIVMWEVMSFGERPYWDMSNQDVINAIEQDYRLPPPPDCPTSLHQLMLDCWQKDRNARPRFPQVVSALDKMIRNPASLKIVARENGGASHPLLDQRQPHYSAFGSVGEWLRAIKMGRYEESFAAAGFGSFEVVSQISAEDLLRIGVTLAGHQKKILASVQHMKSQAKPGAPGGTGGPAQQF

Target ORF information:

RefSeq Version	NM_010144.6
Organism	Mus musculus(house mouse)
Definition	Mus musculus Eph receptor B4 (Ephb4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010144.6

ORF Insert Sequence:

ATGGAGCTCC GAGCGCTGCT GTGCTGGGCT TCCCTCGCCA CTGCTTTAGA AGAGACCCTG 
TTGAACACAA AACTGGAAAC GGCGGATCTG AAATGGGTGA CTTACCCTCA GGCAGAGGGC 
CAGTGGGAGG AGCTAAGCGG CCTGGATGAG GAACAGCACA GCGTCCGCAC CTATGAGGTG 
TGCGACATGA AGCGTCCAGG GGGCCAGGCT CACTGGCTGC GCACTGGCTG GGTCCCAAGG 
CGAGGTGCTG TCCACGTGTA TGCCACGATA CGCTTCACCA TGATGGAATG CCTGTCCCTG 
CCGAGGGCCA GTCGCTCCTG CAAGGAGACA TTCACTGTCT TCTATTACGA GAGCGAAGCT 
GATACGGCCA CGGCCCATAC GCCCGCCTGG ATGGAGAACC CCTACATCAA GGTGGACACA 
GTGGCCGCAG AACATCTGAC TCGGAAGCGC CCTGGAGCTG AAGCCACAGG GAAAGTTAAT 
ATCAAGACGC TGCGCCTGGG TCCTCTCAGC AAAGCTGGCT TCTACCTGGC TTTCCAGGAC 
CAAGGAGCCT GCATGGCTCT GCTCTCCCTG CATCTCTTTT ACAAGAAGTG CTCCTGGCTG 
ATCACGAACT TGACCTACTT CCCCGAGACG GTGCCTCGGG AGCTCGTGGT GCCGGTGGCA 
GGTAGCTGCG TGGCCAACGC GGTCCCTACC GCCAACCCCA GCCCCAGCCT CTACTGCCGG 
GAAGATGGTC AATGGGCTGA GCAGCAGGTC ACGGGCTGCA GCTGCGCGCC AGGGTACGAG 
GCTGCGGAAA GCAACAAAGT ATGCAGAGCC TGTGGCCAGG GAACCTTCAA GCCCCAAATA 
GGAGACGAGT CCTGCCTGCC ATGCCCAGCC AACAGCCACT CGAATAACAT TGGGTCTCCT 
GTCTGCCTGT GTCGAATTGG GTATTACCGG GCCCGCTCAG ACCCCCGGAG TTCACCTTGC 
ACTACCCCAC CCTCTGCTCC AAGAAGCGTG GTTCACCATT TGAATGGTTC CACCCTGCGC 
CTGGAATGGA GTGCTCCCCT TGAGTCCGGA GGCCGAGAGG ACCTCACTTA TGCTGTACGC 
TGCCGAGAGT GCCGTCCTGG GGGTTCCTGC TTGCCCTGTG GGGGCGACAT GACCTTCGAC 
CCCGGTCCTC GAGACCTGGT TGAGCCCTGG GTGGCAATCC GAGGGCTGCG TCCTGATGTC 
ACCTATACCT TTGAGGTTGC TGCTTTGAAT GGTGTGTCTA CCTTAGCCAC TGGACCACCT 
CCTTTTGAGC CTGTCAATGT CACCACTGAC CGTGAGGTGC CTCCTGCAGT GTCTGACATC 
CGAGTGACTC GGTCGTCACC CAGCAGCTTG ATCCTGTCAT GGGCTATCCC CAGAGCACCC 
AGTGGGGCCG TGCTGGACTA CGAGGTCAAG TATCATGAGA AGGGCGCAGA GGGCCCCAGC 
AGTGTTCGTT TCCTGAAGAC ATCAGAAAAC CGAGCTGAGC TCCGGGGGCT GAAGCGGGGA 
GCCAGCTATC TGGTCCAGGT ACGCGCACGG TCCGAGGCTG GCTACGGTCC CTTCGGCCAG 
GAGCATCACA GTCAGACTCA ACTGGATGAG AGCGAGAGCT GGCGGGAGCA GCTGGCCCTG 
ATTGCAGGCA CTGCGGTTGT GGGTGTGGTC CTGGTCCTGG TGGTCGTCAT CATTGCAGTT 
CTCTGCCTCA GGAAGCAGAG CAATGGGAGG GAAGTTGAGT ACTCGGATAA GCATGGGCAG 
TATCTCATCG GGCACGGTAC CAAGGTCTAC ATTGATCCTT TTACTTACGA AGACCCTAAT 
GAGGCAGTGA GGGAATTTGC CAAAGAGATC GATGTCTCCT ATGTCAAGAT TGAAGAGGTA 
ATTGGTGCAG GTGAGTTCGG CGAGGTGTGC CGGGGTCGGC TGAAGGCACC AGGGAAAAAG 
GAGAGCTGTG TGGCCATCAA GACTCTGAAG GGTGGCTACA CCGAGCGCCA GAGGCGTGAG 
TTCCTGAGCG AGGCCTCCAT CATGGGCCAG TTCGAGCATC CCAACATCAT CCGCCTCGAG 
GGCGTGGTCA CCAACAGTGT GCCGGTTATG ATCCTCACGG AATTCATGGA GAACGGAGCC 
CTGGACTCCT TCCTGCGGCT GAACGACGGG CAGTTCACAG TCATCCAGCT GGTGGGCATG 
CTGAGGGGCA TCGCCTCGGG CATGCGGTAC CTGGCTGAAA TGAGCTATGT CCACCGAGAC 
CTGGCTGCTC GGAACATCTT GGTCAACAGT AACCTGGTCT GCAAGGTGTC CGACTTTGGC 
CTCTCCAGAT TCTTGGAGGA GAACTCCTCT GATCCCACCT ACACAAGTTC CCTGGGAGGC 
AAGATTCCCA TCCGATGGAC CGCCCCTGAA GCCATTGCCT TCAGGAAGTT CACCTCTGCC 
AGTGATGCCT GGAGCTATGG GATCGTCATG TGGGAGGTCA TGTCTTTTGG GGAACGGCCA 
TACTGGGACA TGAGCAACCA GGATGTGATC AATGCCATTG AACAGGACTA CCGGCTGCCT 
CCTCCTCCAG ACTGCCCCAC CTCCCTCCAC CAGCTCATGC TGGATTGTTG GCAGAAGGAC 
CGGAATGCCC GGCCCCGCTT TCCCCAGGTG GTCAGCGCTC TGGACAAGAT GATCCGGAAT 
CCCGCTAGCC TCAAAATCGT GGCCAGGGAG AATGGCGGGG CCTCACATCC ACTCTTGGAC 
CAACGGCAGC CTCACTATTC TGCTTTCGGT TCTGTGGGCG AGTGGCTTCG AGCCATCAAG 
ATGGGAAGAT ACGAGGAAAG TTTTGCAGCG GCTGGATTCG GCTCCTTTGA GGTGGTCAGT 
CAGATCTCTG CCGAGGACCT TCTCCGAATT GGAGTCACTC TGGCAGGACA CCAGAAGAAA 
ATCTTGGCCA GTGTGCAGCA TATGAAGTCC CAAGCTAAGC CAGGAGCCCC TGGTGGGACA 
GGGGGACCAG CCCAGCAGTT CTGA