Epb42 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Epb42
Entrez Gene ID	13828
Full Name	erythrocyte membrane protein band 4.2
Synonyms	Epb4.2
General protein information	Preferred Names erythrocyte membrane protein band 4.2 Names erythrocyte membrane protein band 4.2 P4.2 erythrocyte protein 4.2 erythrocyte protein band 4.2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 E5\|2 60.37 cM
Summary	The protein encoded by this gene is the key component of a macromolecular complex involved in the structure of erythrocytes. [provided by RefSeq, Aug 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_013513.3	NP_038541.1	erythrocyte membrane protein band 4.2
XM_006498727.3	XP_006498790.1	erythrocyte membrane protein band 4.2 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Epb4.2	NP_038541.1
Rattus norvegicus (Norway rat)	Epb42	NP_001102060.1
	LOC709427	XP_002808491.1
Bos taurus (cattle)	EPB42	NP_776737.1
Xenopus tropicalis (tropical clawed frog)	epb42	XP_002935587.2
Homo sapiens (human)	EPB42	NP_000110.2
Pan troglodytes (chimpanzee)	EPB42	XP_510346.2
Canis lupus familiaris (dog)	EPB42	XP_005638380.1
Gallus gallus (chicken)	EPB42	XP_417393.2

Gene Ontology
Bibliography

Related articles in PubMed

Circulating primitive erythroblasts establish a functional, protein 4.1R-dependent cytoskeletal network prior to enucleating.
Huang YS, Delgadillo LF, Cyr KH, Kingsley PD, An X, McGrath KE, Mohandas N, Conboy JG, Waugh RE, Wan J, Palis J
Scientific reports7(1)5164(2017 Jul)

A novel ENU-induced ankyrin-1 mutation impairs parasite invasion and increases erythrocyte clearance during malaria infection in mice.
Huang HM, Bauer DC, Lelliott PM, Greth A, McMorran BJ, Foote SJ, Burgio G
Scientific reports637197(2016 Nov)

Decreased expression levels of Ifi genes is associated to the increased resistance to spontaneous arthritis disease in mice deficiency of IL-1RA.
Liu X, Jiao Y, Cao Y, Deng N, Ma Y, Hasty KA, Kang A, Chen H, Stuart JM, Gu W
BMC immunology17(1)25(2016 08)

Merozoite surface protein 1 recognition of host glycophorin A mediates malaria parasite invasion of red blood cells.
Baldwin MR, Li X, Hanada T, Liu SC, Chishti AH
Blood125(17)2704-11(2015 Apr)

Severe anemia in the Nan mutant mouse caused by sequence-selective disruption of erythroid Kruppel-like factor.
Siatecka M, Sahr KE, Andersen SG, Mezei M, Bieker JJ, Peters LL
Proceedings of the National Academy of Sciences of the United States of America107(34)15151-6(2010 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that a nuclear complex containing TAL1 and LIM domain-binding protein Ldb1 transactivates the protein 4.2 (P4.2) gene
Title: Identification of a TAL1 target gene reveals a positive role for the LIM domain-binding protein Ldb1 in erythroid gene expression and differentiation.

The following Epb42 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Epb42 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu09517	NM_013513.3 Latest version!	Mus musculus erythrocyte membrane protein band 4.2 (Epb42), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu30003	XM_006498727.3 Latest version!	Mus musculus erythrocyte membrane protein band 4.2 (Epb42), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Epb42 ( NM_013513.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038541.1 Mus musculus erythrocyte membrane protein band 4.2 (Epb42), mRNA.

Price & Availability↑

CloneID	OMu09517
Clone ID Related Accession (Same CDS sequence)	NM_013513.3
Accession Version	NM_013513.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	erythrocyte membrane protein band 4.2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY230962.1, AK133896.1 and AK143841.1. On Aug 20, 2015 this sequence version replaced NM_013513.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK143841.1, AK133896.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135, SAMN01164140 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGCAAG CTCTGAGCAT CAAGAGCTGT GACTTCCACG CAGCAGAAAA CAACGAGGAG 
CACTACACCA AGGCCATCAG CTCCCAGCAC CTTACTTTGA GGAGGGGACA GTCCTTCACT 
ATCACCCTGA ACTTCCGTGC TCCAACCCAC ACATTTCTGT CTGCCCTGAA GAAAGTGGCT 
CTCATTGCAC AAACGGGAGA GCAACCTTCC AAGATCAATA AGACCCAAGC CATCTTCCCA 
ATTTCCTCTC TTGGGGACCA AAAAGGGTGG AGTGCAGCAG TGGAGGAAAG AGATGCCCAA 
CACTGGACCG TCTCTGTGAC CACACCTGTG GATGCTGTCA TTGGCCACTA CTCACTCTTG 
CTTCAGGTTT CAGGAAAGAA ACAATACCCC TTGGGCCAAT TCACACTGCT CTTTAATCCA 
TGGAACAGAG ATGATGCTGT ATTTCTACAG AACGAGGCTG AACGCACAGA ATATGTGTTG 
AACCAGAACG GCTTTATCTA CCTGGGCACA GCTGACTGCA TTCAGGAAGA GCCCTGGGAC 
TTTGGTCAGT TTGAGAAAGA CGTCATGGAC CTCAGCCTGA AGTTACTGAG TATGGACAAG 
CAAGTGAAAG ACTGGAACCA GCCTGCACAC GTGGCCCGAG TTGTGGGCGC CTTGTTGCAT 
GCCCTCAAGA AGAAGAGTGT CCTGCCTATT TCACAGACCC AGGCTGCCCA GGAAGGTGCC 
TTGTTGTACA AACGTCGGGG CAGCGTGCCC ATCCTGAGGC AGTGGCTCAC TGGCCAAGGA 
CGAGCCGTGT ATGAGACCCA GGCGTGGGTG TCTGCTGCTG TTGCTTGCAC AGTGCTACGG 
TGCCTGGGCA TCCCAGCTCG AGTCGTTACC ACATTTGATT CAGCCCAAGG TACAGTTGGG 
AGCCTGCTGG TGGATGAGTA CTACAATGAG GAGGGACTCC AGAATGGAGA AGGACAGAGG 
GGCCATATCT GGGTCTTCCA AACGTCTGTA GAATGCTGGA TGAACAGACC TGATTTGTCC 
CAAGGTTATG GTGGATGGCA GATTCTGCAC CCAAGGGCTC CTAATGGAGC TGGAGTCCTG 
GGATCCTGCA GTCTGGTCCC AGTCAGAGCA GTCAAGGAGG GGGAACTGCA GCTGGACCCC 
GCAGTACCAG AGCTCTTTGC AGCAGTAAAT GCCTCGTGTG TGGTCTGGAA GTGCTGTGAG 
GATGGGAAGT TGGAGTTGAC CAACTCCAAC AGAAAAGATG TGGGCAACTG CATCAGCACC 
AAGGTGGTGG GCAGTGACCG CTGTGAAGAC ATCACTCAGA ACTACAAGTA TCCTGCAGGA 
TCTCTTCAAG AAAAGGAGGT ACTGGAGAAA GTCCAAAAAG AAAGACTGAA GCTTGGGAAA 
GACAACGGCA TGTGTCCTCC CAGTTGTGAG CCCTGGGATC CTCTACACAT GTTCTTCGAA 
GCATCTAGCT CCATCCCTCT GAGTGGAGAT GGTCAGCTCT CAGTGACCTT GATTAACCCC 
ACAGACGAGG AGAAGAAGGT GCACCTGGTG ATTGGGGCCC AGGCTTTGTA CTACAATGGA 
GTCCTCGCTG CTGGTCTCTG GAGCAAGAAG CAGTTGTTCA TGCTCAAACC CAACCAGGTG 
ATGAGACTAT CCACCAACCT GTCCTTCTCC TGTTTTGAGC AAACCCCACC CGAGAACAGC 
TTTCTCCGAG TCACGGCAAT GGCAAGGTAC TCCCACACCA GCCTCAGCTG CTTTGCCCAG 
GAAAATATGG CCATTGGTAA ACCGGATCTT ATCATCGAGA TGCCAAAGAG AGCAGCACAG 
TATCGGCCCC TCACTGTCTC CGTTAGAATG CACAACTCCT TAGAGGCCCC CATGCAGAAC 
TGCATCATCT CCATCTTTGG AAGGGGACTC ATTCACAGAG AGAAGAGATA TGGATTGGGT 
TCTCTGTGGC CAGGAAGCAG CCTACATACT CAGTTCCAGT TCACCCCAAC ACATCTGGGG 
CTCCAAAGGC TCACTGTGGA AGTAGATTGT GACATGTTCC AGAACCTGAC CGGCTACAGA 
AGTGTCCTTG TGGTGGCCCC TGAAGTTTCT GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038541.1
CDS	285..2360
Translation	MGQALSIKSCDFHAAENNEEHYTKAISSQHLTLRRGQSFTITLNFRAPTHTFLSALKKVALIAQTGEQPSKINKTQAIFPISSLGDQKGWSAAVEERDAQHWTVSVTTPVDAVIGHYSLLLQVSGKKQYPLGQFTLLFNPWNRDDAVFLQNEAERTEYVLNQNGFIYLGTADCIQEEPWDFGQFEKDVMDLSLKLLSMDKQVKDWNQPAHVARVVGALLHALKKKSVLPISQTQAAQEGALLYKRRGSVPILRQWLTGQGRAVYETQAWVSAAVACTVLRCLGIPARVVTTFDSAQGTVGSLLVDEYYNEEGLQNGEGQRGHIWVFQTSVECWMNRPDLSQGYGGWQILHPRAPNGAGVLGSCSLVPVRAVKEGELQLDPAVPELFAAVNASCVVWKCCEDGKLELTNSNRKDVGNCISTKVVGSDRCEDITQNYKYPAGSLQEKEVLEKVQKERLKLGKDNGMCPPSCEPWDPLHMFFEASSSIPLSGDGQLSVTLINPTDEEKKVHLVIGAQALYYNGVLAAGLWSKKQLFMLKPNQVMRLSTNLSFSCFEQTPPENSFLRVTAMARYSHTSLSCFAQENMAIGKPDLIIEMPKRAAQYRPLTVSVRMHNSLEAPMQNCIISIFGRGLIHREKRYGLGSLWPGSSLHTQFQFTPTHLGLQRLTVEVDCDMFQNLTGYRSVLVVAPEVSV

Target ORF information:

RefSeq Version	NM_013513.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus erythrocyte membrane protein band 4.2 (Epb42), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013513.3

ORF Insert Sequence:

ATGGGGCAAG CTCTGAGCAT CAAGAGCTGT GACTTCCACG CAGCAGAAAA CAACGAGGAG 
CACTACACCA AGGCCATCAG CTCCCAGCAC CTTACTTTGA GGAGGGGACA GTCCTTCACT 
ATCACCCTGA ACTTCCGTGC TCCAACCCAC ACATTTCTGT CTGCCCTGAA GAAAGTGGCT 
CTCATTGCAC AAACGGGAGA GCAACCTTCC AAGATCAATA AGACCCAAGC CATCTTCCCA 
ATTTCCTCTC TTGGGGACCA AAAAGGGTGG AGTGCAGCAG TGGAGGAAAG AGATGCCCAA 
CACTGGACCG TCTCTGTGAC CACACCTGTG GATGCTGTCA TTGGCCACTA CTCACTCTTG 
CTTCAGGTTT CAGGAAAGAA ACAATACCCC TTGGGCCAAT TCACACTGCT CTTTAATCCA 
TGGAACAGAG ATGATGCTGT ATTTCTACAG AACGAGGCTG AACGCACAGA ATATGTGTTG 
AACCAGAACG GCTTTATCTA CCTGGGCACA GCTGACTGCA TTCAGGAAGA GCCCTGGGAC 
TTTGGTCAGT TTGAGAAAGA CGTCATGGAC CTCAGCCTGA AGTTACTGAG TATGGACAAG 
CAAGTGAAAG ACTGGAACCA GCCTGCACAC GTGGCCCGAG TTGTGGGCGC CTTGTTGCAT 
GCCCTCAAGA AGAAGAGTGT CCTGCCTATT TCACAGACCC AGGCTGCCCA GGAAGGTGCC 
TTGTTGTACA AACGTCGGGG CAGCGTGCCC ATCCTGAGGC AGTGGCTCAC TGGCCAAGGA 
CGAGCCGTGT ATGAGACCCA GGCGTGGGTG TCTGCTGCTG TTGCTTGCAC AGTGCTACGG 
TGCCTGGGCA TCCCAGCTCG AGTCGTTACC ACATTTGATT CAGCCCAAGG TACAGTTGGG 
AGCCTGCTGG TGGATGAGTA CTACAATGAG GAGGGACTCC AGAATGGAGA AGGACAGAGG 
GGCCATATCT GGGTCTTCCA AACGTCTGTA GAATGCTGGA TGAACAGACC TGATTTGTCC 
CAAGGTTATG GTGGATGGCA GATTCTGCAC CCAAGGGCTC CTAATGGAGC TGGAGTCCTG 
GGATCCTGCA GTCTGGTCCC AGTCAGAGCA GTCAAGGAGG GGGAACTGCA GCTGGACCCC 
GCAGTACCAG AGCTCTTTGC AGCAGTAAAT GCCTCGTGTG TGGTCTGGAA GTGCTGTGAG 
GATGGGAAGT TGGAGTTGAC CAACTCCAAC AGAAAAGATG TGGGCAACTG CATCAGCACC 
AAGGTGGTGG GCAGTGACCG CTGTGAAGAC ATCACTCAGA ACTACAAGTA TCCTGCAGGA 
TCTCTTCAAG AAAAGGAGGT ACTGGAGAAA GTCCAAAAAG AAAGACTGAA GCTTGGGAAA 
GACAACGGCA TGTGTCCTCC CAGTTGTGAG CCCTGGGATC CTCTACACAT GTTCTTCGAA 
GCATCTAGCT CCATCCCTCT GAGTGGAGAT GGTCAGCTCT CAGTGACCTT GATTAACCCC 
ACAGACGAGG AGAAGAAGGT GCACCTGGTG ATTGGGGCCC AGGCTTTGTA CTACAATGGA 
GTCCTCGCTG CTGGTCTCTG GAGCAAGAAG CAGTTGTTCA TGCTCAAACC CAACCAGGTG 
ATGAGACTAT CCACCAACCT GTCCTTCTCC TGTTTTGAGC AAACCCCACC CGAGAACAGC 
TTTCTCCGAG TCACGGCAAT GGCAAGGTAC TCCCACACCA GCCTCAGCTG CTTTGCCCAG 
GAAAATATGG CCATTGGTAA ACCGGATCTT ATCATCGAGA TGCCAAAGAG AGCAGCACAG 
TATCGGCCCC TCACTGTCTC CGTTAGAATG CACAACTCCT TAGAGGCCCC CATGCAGAAC 
TGCATCATCT CCATCTTTGG AAGGGGACTC ATTCACAGAG AGAAGAGATA TGGATTGGGT 
TCTCTGTGGC CAGGAAGCAG CCTACATACT CAGTTCCAGT TCACCCCAAC ACATCTGGGG 
CTCCAAAGGC TCACTGTGGA AGTAGATTGT GACATGTTCC AGAACCTGAC CGGCTACAGA 
AGTGTCCTTG TGGTGGCCCC TGAAGTTTCT GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Epb42 ( XM_006498727.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498790.1 Mus musculus erythrocyte membrane protein band 4.2 (Epb42), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu30003
Clone ID Related Accession (Same CDS sequence)	XM_006498727.3
Accession Version	XM_006498727.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	erythrocyte membrane protein band 4.2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006498727.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTCAG CTCTGAGCAT CAAGAGCTGT GACTTCCACG CAGCAGAAAA CAACGAGGAG 
CACTACACCA AGGCCATCAG CTCCCAGCAC CTTACTTTGA GGAGGGGACA GTCCTTCACT 
ATCACCCTGA ACTTCCGTGC TCCAACCCAC ACATTTCTGT CTGCCCTGAA GAAAGTGGCT 
CTCATTGCAC AAACGGGAGA GCAACCTTCC AAGATCAATA AGACCCAAGC CATCTTCCCA 
ATTTCCTCTC TTGGGGACCA AAAAGGGTGG AGTGCAGCAG TGGAGGAAAG AGATGCCCAA 
CACTGGACCG TCTCTGTGAC CACACCTGTG GATGCTGTCA TTGGCCACTA CTCACTCTTG 
CTTCAGGTTT CAGGAAAGAA ACAATACCCC TTGGGCCAAT TCACACTGCT CTTTAATCCA 
TGGAACAGAG ATGATGCTGT ATTTCTACAG AACGAGGCTG AACGCACAGA ATATGTGTTG 
AACCAGAACG GCTTTATCTA CCTGGGCACA GCTGACTGCA TTCAGGAAGA GCCCTGGGAC 
TTTGGTCAGT TTGAGAAAGA CGTCATGGAC CTCAGCCTGA AGTTACTGAG TATGGACAAG 
CAAGTGAAAG ACTGGAACCA GCCTGCACAC GTGGCCCGAG TTGTGGGCGC CTTGTTGCAT 
GCCCTCAAGA AGAAGAGTGT CCTGCCTATT TCACAGACCC AGGCTGCCCA GGAAGGTGCC 
TTGTTGTACA AACGTCGGGG CAGCGTGCCC ATCCTGAGGC AGTGGCTCAC TGGCCAAGGA 
CGAGCCGTGT ATGAGACCCA GGCGTGGGTG TCTGCTGCTG TTGCTTGCAC AGTGCTACGG 
TGCCTGGGCA TCCCAGCTCG AGTCGTTACC ACATTTGATT CAGCCCAAGG TACAGTTGGG 
AGCCTGCTGG TGGATGAGTA CTACAATGAG GAGGGACTCC AGAATGGAGA AGGACAGAGG 
GGCCATATCT GGGTCTTCCA AACGTCTGTA GAATGCTGGA TGAACAGACC TGATTTGTCC 
CAAGGTTATG GTGGATGGCA GATTCTGCAC CCAAGGGCTC CTAATGGAGC TGGAGTCCTG 
GGATCCTGCA GTCTGGTCCC AGTCAGAGCA GTCAAGGAGG GGGAACTGCA GCTGGACCCC 
GCAGTACCAG AGCTCTTTGC AGCAGTAAAT GCCTCGTGTG TGGTCTGGAA GTGCTGTGAG 
GATGGGAAGT TGGAGTTGAC CAACTCCAAC AGAAAAGATG TGGGCAACTG CATCAGCACC 
AAGGTGGTGG GCAGTGACCG CTGTGAAGAC ATCACTCAGA ACTACAAGTA TCCTGCAGGA 
TCTCTTCAAG AAAAGGAGGT ACTGGAGAAA GTCCAAAAAG AAAGACTGAA GCTTGGGAAA 
GACAACGGCA TGTGTCCTCC CAGTTGTGAG CCCTGGGATC CTCTACACAT GTTCTTCGAA 
GCATCTAGCT CCATCCCTCT GAGTGGAGAT GGTCAGCTCT CAGTGACCTT GATTAACCCC 
ACAGACGAGG AGAAGAAGGT GCACCTGGTG ATTGGGGCCC AGGCTTTGTA CTACAATGGA 
GTCCTCGCTG CTGGTCTCTG GAGCAAGAAG CAGTTGTTCA TGCTCAAACC CAACCAGGTG 
ATGAGACTAT CCACCAACCT GTCCTTCTCC TGTTTTGAGC AAACCCCACC CGAGAACAGC 
TTTCTCCGAG TCACGGCAAT GGCAAGGTAC TCCCACACCA GCCTCAGCTG CTTTGCCCAG 
GAAAATATGG CCATTGGTAA ACCGGATCTT ATCATCGAGA TGCCAAAGAG AGCAGCACAG 
TATCGGCCCC TCACTGTCTC CGTTAGAATG CACAACTCCT TAGAGGCCCC CATGCAGAAC 
TGCATCATCT CCATCTTTGG AAGGGGACTC ATTCACAGAG AGAAGAGATA TGGATTGGGT 
TCTCTGTGGC CAGGAAGCAG CCTACATACT CAGTTCCAGT TCACCCCAAC ACATCTGGGG 
CTCCAAAGGC TCACTGTGGA AGTAGATTGT GACATGTTCC AGAACCTGAC CGGCTACAGA 
AGTGTCCTTG TGGTGGCCCC TGAAGTTTCT GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498790.1
CDS	224..2299
Translation	MISALSIKSCDFHAAENNEEHYTKAISSQHLTLRRGQSFTITLNFRAPTHTFLSALKKVALIAQTGEQPSKINKTQAIFPISSLGDQKGWSAAVEERDAQHWTVSVTTPVDAVIGHYSLLLQVSGKKQYPLGQFTLLFNPWNRDDAVFLQNEAERTEYVLNQNGFIYLGTADCIQEEPWDFGQFEKDVMDLSLKLLSMDKQVKDWNQPAHVARVVGALLHALKKKSVLPISQTQAAQEGALLYKRRGSVPILRQWLTGQGRAVYETQAWVSAAVACTVLRCLGIPARVVTTFDSAQGTVGSLLVDEYYNEEGLQNGEGQRGHIWVFQTSVECWMNRPDLSQGYGGWQILHPRAPNGAGVLGSCSLVPVRAVKEGELQLDPAVPELFAAVNASCVVWKCCEDGKLELTNSNRKDVGNCISTKVVGSDRCEDITQNYKYPAGSLQEKEVLEKVQKERLKLGKDNGMCPPSCEPWDPLHMFFEASSSIPLSGDGQLSVTLINPTDEEKKVHLVIGAQALYYNGVLAAGLWSKKQLFMLKPNQVMRLSTNLSFSCFEQTPPENSFLRVTAMARYSHTSLSCFAQENMAIGKPDLIIEMPKRAAQYRPLTVSVRMHNSLEAPMQNCIISIFGRGLIHREKRYGLGSLWPGSSLHTQFQFTPTHLGLQRLTVEVDCDMFQNLTGYRSVLVVAPEVSV

Target ORF information:

RefSeq Version	XM_006498727.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus erythrocyte membrane protein band 4.2 (Epb42), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498727.3

ORF Insert Sequence:

ATGATCTCAG CTCTGAGCAT CAAGAGCTGT GACTTCCACG CAGCAGAAAA CAACGAGGAG 
CACTACACCA AGGCCATCAG CTCCCAGCAC CTTACTTTGA GGAGGGGACA GTCCTTCACT 
ATCACCCTGA ACTTCCGTGC TCCAACCCAC ACATTTCTGT CTGCCCTGAA GAAAGTGGCT 
CTCATTGCAC AAACGGGAGA GCAACCTTCC AAGATCAATA AGACCCAAGC CATCTTCCCA 
ATTTCCTCTC TTGGGGACCA AAAAGGGTGG AGTGCAGCAG TGGAGGAAAG AGATGCCCAA 
CACTGGACCG TCTCTGTGAC CACACCTGTG GATGCTGTCA TTGGCCACTA CTCACTCTTG 
CTTCAGGTTT CAGGAAAGAA ACAATACCCC TTGGGCCAAT TCACACTGCT CTTTAATCCA 
TGGAACAGAG ATGATGCTGT ATTTCTACAG AACGAGGCTG AACGCACAGA ATATGTGTTG 
AACCAGAACG GCTTTATCTA CCTGGGCACA GCTGACTGCA TTCAGGAAGA GCCCTGGGAC 
TTTGGTCAGT TTGAGAAAGA CGTCATGGAC CTCAGCCTGA AGTTACTGAG TATGGACAAG 
CAAGTGAAAG ACTGGAACCA GCCTGCACAC GTGGCCCGAG TTGTGGGCGC CTTGTTGCAT 
GCCCTCAAGA AGAAGAGTGT CCTGCCTATT TCACAGACCC AGGCTGCCCA GGAAGGTGCC 
TTGTTGTACA AACGTCGGGG CAGCGTGCCC ATCCTGAGGC AGTGGCTCAC TGGCCAAGGA 
CGAGCCGTGT ATGAGACCCA GGCGTGGGTG TCTGCTGCTG TTGCTTGCAC AGTGCTACGG 
TGCCTGGGCA TCCCAGCTCG AGTCGTTACC ACATTTGATT CAGCCCAAGG TACAGTTGGG 
AGCCTGCTGG TGGATGAGTA CTACAATGAG GAGGGACTCC AGAATGGAGA AGGACAGAGG 
GGCCATATCT GGGTCTTCCA AACGTCTGTA GAATGCTGGA TGAACAGACC TGATTTGTCC 
CAAGGTTATG GTGGATGGCA GATTCTGCAC CCAAGGGCTC CTAATGGAGC TGGAGTCCTG 
GGATCCTGCA GTCTGGTCCC AGTCAGAGCA GTCAAGGAGG GGGAACTGCA GCTGGACCCC 
GCAGTACCAG AGCTCTTTGC AGCAGTAAAT GCCTCGTGTG TGGTCTGGAA GTGCTGTGAG 
GATGGGAAGT TGGAGTTGAC CAACTCCAAC AGAAAAGATG TGGGCAACTG CATCAGCACC 
AAGGTGGTGG GCAGTGACCG CTGTGAAGAC ATCACTCAGA ACTACAAGTA TCCTGCAGGA 
TCTCTTCAAG AAAAGGAGGT ACTGGAGAAA GTCCAAAAAG AAAGACTGAA GCTTGGGAAA 
GACAACGGCA TGTGTCCTCC CAGTTGTGAG CCCTGGGATC CTCTACACAT GTTCTTCGAA 
GCATCTAGCT CCATCCCTCT GAGTGGAGAT GGTCAGCTCT CAGTGACCTT GATTAACCCC 
ACAGACGAGG AGAAGAAGGT GCACCTGGTG ATTGGGGCCC AGGCTTTGTA CTACAATGGA 
GTCCTCGCTG CTGGTCTCTG GAGCAAGAAG CAGTTGTTCA TGCTCAAACC CAACCAGGTG 
ATGAGACTAT CCACCAACCT GTCCTTCTCC TGTTTTGAGC AAACCCCACC CGAGAACAGC 
TTTCTCCGAG TCACGGCAAT GGCAAGGTAC TCCCACACCA GCCTCAGCTG CTTTGCCCAG 
GAAAATATGG CCATTGGTAA ACCGGATCTT ATCATCGAGA TGCCAAAGAG AGCAGCACAG 
TATCGGCCCC TCACTGTCTC CGTTAGAATG CACAACTCCT TAGAGGCCCC CATGCAGAAC 
TGCATCATCT CCATCTTTGG AAGGGGACTC ATTCACAGAG AGAAGAGATA TGGATTGGGT 
TCTCTGTGGC CAGGAAGCAG CCTACATACT CAGTTCCAGT TCACCCCAAC ACATCTGGGG 
CTCCAAAGGC TCACTGTGGA AGTAGATTGT GACATGTTCC AGAACCTGAC CGGCTACAGA 
AGTGTCCTTG TGGTGGCCCC TGAAGTTTCT GTTTAA