Eif2b4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Eif2b4
Entrez Gene ID	13667
Full Name	eukaryotic translation initiation factor 2B, subunit 4 delta
Synonyms	Eif2b
General protein information	Preferred Names eukaryotic translation initiation factor 2B, subunit 4 delta Names translation initiation factor eIF-2B subunit delta eIF-2B GDP-GTP exchange factor subunit delta
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001127356.1	NP_001120828.1	translation initiation factor eIF-2B subunit delta isoform 1
NM_010122.2	NP_034252.2	translation initiation factor eIF-2B subunit delta isoform 2
NM_001127355.1	NP_001120827.1	translation initiation factor eIF-2B subunit delta isoform 1

Pathways from BioSystems

Homologies

	EIF2B4	XP_001094256.2
Gallus gallus (chicken)	EIF2B4	XP_423512.2
Pan troglodytes (chimpanzee)	EIF2B4	XP_515356.2
Rattus norvegicus (Norway rat)	Eif2b4	NP_446402.1
Xenopus tropicalis (tropical clawed frog)	eif2b4	NP_001072364.1
Homo sapiens (human)	EIF2B4	NP_001029288.1
Bos taurus (cattle)	EIF2B4	NP_001029716.1
Mus musculus (house mouse)	Eif2b4	NP_034252.2
Danio rerio (zebrafish)	eif2b4	NP_001014383.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005937	XP_556607.3
Canis lupus familiaris (dog)	EIF2B4	XP_860642.1
Arabidopsis thaliana (thale cress)	AT5G38640	NP_198680.1
Oryza sativa (rice)	Os12g0617100	NP_001067287.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Astrocytes are central in the pathomechanisms of vanishing white matter.
Dooves S, Bugiani M, Postma NL, Polder E, Land N, Horan ST, van Deijk AL, van de Kreeke A, Jacobs G, Vuong C, Klooster J, Kamermans M, Wortel J, Loos M, Wisse LE, Scheper GC, Abbink TE, Heine VM, van der Knaap MS
The Journal of clinical investigation126(4)1512-24(2016 Apr)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

A novel interaction between adrenergic receptors and the alpha-subunit of eukaryotic initiation factor 2B.
Klein U, Ramirez MT, Kobilka BK, von Zastrow M
The Journal of biological chemistry272(31)19099-102(1997 Aug)

The delta-subunit of murine guanine nucleotide exchange factor eIF-2B. Characterization of cDNAs predicts isoforms differing at the amino-terminal end.
Henderson RA, Krissansen GW, Yong RY, Leung E, Watson JD, Dholakia JN
The Journal of biological chemistry269(48)30517-23(1994 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Eif2b4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eif2b4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu05931	NM_001127355.1 Latest version!	Mus musculus eukaryotic translation initiation factor 2B, subunit 4 delta (Eif2b4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu05931	NM_001127356.1 Latest version!	Mus musculus eukaryotic translation initiation factor 2B, subunit 4 delta (Eif2b4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu05577	NM_010122.2 Latest version!	Mus musculus eukaryotic translation initiation factor 2B, subunit 4 delta (Eif2b4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Eif2b4 ( NM_001127356.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001120828.1 Mus musculus eukaryotic translation initiation factor 2B, subunit 4 delta (Eif2b4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu05931
Clone ID Related Accession (Same CDS sequence)	NM_001127356.1 , NM_001127355.1
Accession Version	NM_001127356.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Mus musculus(house mouse)
Product	translation initiation factor eIF-2B subunit delta isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB665023.1, BU707309.1, AK158282.1 and CD768292.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Variants 1 and 3 encode the same isoform. ##Evidence-Data-START## Transcript exon combination :: M98035.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCCAACCC AGCAGCCAGC TGCGCCGACG AGTCTCCCCA AGTCCTCCCG GAGCCTGTCT 
GGCTCACTCT GTGCCCTGTT TTCTGATGCA GAATCGAGAT CCGAGATGAA GACTGAACTT 
TCTCCTCGGC CCGGGGCAGC AGGGCGGGAG TTGACCCAAG AAGAGAAGCT TCAGCTTCGA 
AAAGAAAAGA AACAGCAGAA GAAGAAACGG AAGGAGGAAA AGGGGGCAGA CCAAGAAATT 
GGCTCTGCTG TGTCTGCAGC TCAACGGCAA GACCCAATCA GAGAACTCCC AGGACCTGGT 
AGTCAGCTGG GAGGTACTGC TGGGGAGAAA CTTCCAGCTG GCCGGAGTAA GGCAGAACTT 
CGCGCCGAAA GGAGAGCCAA GCAGGAGGCA GAGCGGGCCC TGAAACAGGC AAGAAAAGGG 
GAACAAGGAG GCGTCCCTCC TCAGGCCTGC CCCAGCACTG CTGGAGAAAC CACCTCAGGA 
GTGAAGCGTG TCCCCGAGCA CACCCCAGCT GATGACCCCA CACTTCTGAG GAGGCTCCTT 
AGAAAGCCAG ATCGACAACA GGTTCCTACA CGAAAGGATT ACGGATCCAA AGTCAGTCTC 
TTCTCCCACC TGCCTCAGTA CAGCAGACAA AGCTCCTTGA CCCAGTACAT GAGCATCCCA 
TCCTCTGTGA TCCACCCAGC CATGGTGAGA CTCGGTCTGC AGTACTCCCA GGGCCTCATC 
AGTGGCTCCA ATGCCCGGTG CATCGCACTG CTTCACGCCC TGCAGCAGGT GATTCAGGAT 
TACACAACAC CTCCCAGTGA GGAACTCTCC AGGGATCTTG TAAATAAACT AAAACCCTAC 
ATCAGCTTCC TGACGCAGTG CCGCCCCATG TCGGCAAGTA TGTGCAACGC CATCAAGTTC 
CTAACCAAGG AAGTCACTGG CATGAGCAGC TCCAAGCGGG AAGAGGAGGC CAAGTCAGAA 
CTTAGAGAAG CCCTCGACCG GTACGTGCAA GAGAAGATCG TGCTTGCAGC TCAGGCAATT 
TCACGATTTG CTTCTACAAA GATCAGTGAT GGGGACGTGA TCCTGGTATA TGGATGCTCA 
TCTTTGGTGT CGAGAATTCT CCAGGAGGCC AGGGTCGAGG GCAGGCGGTT TCGGGTGGTG 
GTGGTGGACA GCCGGCCCCG GCTGGAAGGA AGGCACATGC TCCACTCTCT TGTCCGTGCT 
GGGGTCCCTA CCTCCTATCT GCTGATTCCT GCAGCCTCCT ATGTGCTTCC AGAGGTTTCT 
AAGGTGCTAT TGGGAGCTCA TGCACTCCTG GCCAATGGAT CTGTGATGTC TCGGGTAGGG 
ACAGCACAGT TGGCCCTGGT GGCTCGAGCT CATAACGTTC CAGTACTGGT CTGCTGTGAA 
ACGTACAAGT TCTGTGAACG TGTGCAGACT GATGCCTTTG TCTCCAACGA GCTAGATGAT 
CCCGATGATC TGCAGTGTAA GCGGGGAGAC CAGGTGGCCC TGGCTAACTG GCAGAGCCAC 
CCGTCCCTCC GGTTGTTGAA TCTGGTCTAT GACGTGACTC CACCTGAGCT CGTGGATCTG 
GTGATCACAG AGTTGGGCAT GATCCCTTGC AGTTCTGTGC CTGTTGTTCT TCGAGTCAAG 
AGCAGTGACC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001120828.1
CDS	424..2058
Translation	MPTQQPAAPTSLPKSSRSLSGSLCALFSDAESRSEMKTELSPRPGAAGRELTQEEKLQLRKEKKQQKKKRKEEKGADQEIGSAVSAAQRQDPIRELPGPGSQLGGTAGEKLPAGRSKAELRAERRAKQEAERALKQARKGEQGGVPPQACPSTAGETTSGVKRVPEHTPADDPTLLRRLLRKPDRQQVPTRKDYGSKVSLFSHLPQYSRQSSLTQYMSIPSSVIHPAMVRLGLQYSQGLISGSNARCIALLHALQQVIQDYTTPPSEELSRDLVNKLKPYISFLTQCRPMSASMCNAIKFLTKEVTGMSSSKREEEAKSELREALDRYVQEKIVLAAQAISRFASTKISDGDVILVYGCSSLVSRILQEARVEGRRFRVVVVDSRPRLEGRHMLHSLVRAGVPTSYLLIPAASYVLPEVSKVLLGAHALLANGSVMSRVGTAQLALVARAHNVPVLVCCETYKFCERVQTDAFVSNELDDPDDLQCKRGDQVALANWQSHPSLRLLNLVYDVTPPELVDLVITELGMIPCSSVPVVLRVKSSDQ

Target ORF information:

RefSeq Version	NM_001127356.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 2B, subunit 4 delta (Eif2b4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127356.1

ORF Insert Sequence:

ATGCCAACCC AGCAGCCAGC TGCGCCGACG AGTCTCCCCA AGTCCTCCCG GAGCCTGTCT 
GGCTCACTCT GTGCCCTGTT TTCTGATGCA GAATCGAGAT CCGAGATGAA GACTGAACTT 
TCTCCTCGGC CCGGGGCAGC AGGGCGGGAG TTGACCCAAG AAGAGAAGCT TCAGCTTCGA 
AAAGAAAAGA AACAGCAGAA GAAGAAACGG AAGGAGGAAA AGGGGGCAGA CCAAGAAATT 
GGCTCTGCTG TGTCTGCAGC TCAACGGCAA GACCCAATCA GAGAACTCCC AGGACCTGGT 
AGTCAGCTGG GAGGTACTGC TGGGGAGAAA CTTCCAGCTG GCCGGAGTAA GGCAGAACTT 
CGCGCCGAAA GGAGAGCCAA GCAGGAGGCA GAGCGGGCCC TGAAACAGGC AAGAAAAGGG 
GAACAAGGAG GCGTCCCTCC TCAGGCCTGC CCCAGCACTG CTGGAGAAAC CACCTCAGGA 
GTGAAGCGTG TCCCCGAGCA CACCCCAGCT GATGACCCCA CACTTCTGAG GAGGCTCCTT 
AGAAAGCCAG ATCGACAACA GGTTCCTACA CGAAAGGATT ACGGATCCAA AGTCAGTCTC 
TTCTCCCACC TGCCTCAGTA CAGCAGACAA AGCTCCTTGA CCCAGTACAT GAGCATCCCA 
TCCTCTGTGA TCCACCCAGC CATGGTGAGA CTCGGTCTGC AGTACTCCCA GGGCCTCATC 
AGTGGCTCCA ATGCCCGGTG CATCGCACTG CTTCACGCCC TGCAGCAGGT GATTCAGGAT 
TACACAACAC CTCCCAGTGA GGAACTCTCC AGGGATCTTG TAAATAAACT AAAACCCTAC 
ATCAGCTTCC TGACGCAGTG CCGCCCCATG TCGGCAAGTA TGTGCAACGC CATCAAGTTC 
CTAACCAAGG AAGTCACTGG CATGAGCAGC TCCAAGCGGG AAGAGGAGGC CAAGTCAGAA 
CTTAGAGAAG CCCTCGACCG GTACGTGCAA GAGAAGATCG TGCTTGCAGC TCAGGCAATT 
TCACGATTTG CTTCTACAAA GATCAGTGAT GGGGACGTGA TCCTGGTATA TGGATGCTCA 
TCTTTGGTGT CGAGAATTCT CCAGGAGGCC AGGGTCGAGG GCAGGCGGTT TCGGGTGGTG 
GTGGTGGACA GCCGGCCCCG GCTGGAAGGA AGGCACATGC TCCACTCTCT TGTCCGTGCT 
GGGGTCCCTA CCTCCTATCT GCTGATTCCT GCAGCCTCCT ATGTGCTTCC AGAGGTTTCT 
AAGGTGCTAT TGGGAGCTCA TGCACTCCTG GCCAATGGAT CTGTGATGTC TCGGGTAGGG 
ACAGCACAGT TGGCCCTGGT GGCTCGAGCT CATAACGTTC CAGTACTGGT CTGCTGTGAA 
ACGTACAAGT TCTGTGAACG TGTGCAGACT GATGCCTTTG TCTCCAACGA GCTAGATGAT 
CCCGATGATC TGCAGTGTAA GCGGGGAGAC CAGGTGGCCC TGGCTAACTG GCAGAGCCAC 
CCGTCCCTCC GGTTGTTGAA TCTGGTCTAT GACGTGACTC CACCTGAGCT CGTGGATCTG 
GTGATCACAG AGTTGGGCAT GATCCCTTGC AGTTCTGTGC CTGTTGTTCT TCGAGTCAAG 
AGCAGTGACC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eif2b4 ( NM_001127355.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001120827.1 Mus musculus eukaryotic translation initiation factor 2B, subunit 4 delta (Eif2b4), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu05931
Clone ID Related Accession (Same CDS sequence)	NM_001127356.1 , NM_001127355.1
Accession Version	NM_001127355.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Mus musculus(house mouse)
Product	translation initiation factor eIF-2B subunit delta isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB665023.1, BB857319.1, AK158282.1 and CD768292.1. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1, and encodes isoform 1. Variants 1 and 3 encode the same isoform. ##Evidence-Data-START## CDS exon combination :: M98035.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN01164142 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCCAACCC AGCAGCCAGC TGCGCCGACG AGTCTCCCCA AGTCCTCCCG GAGCCTGTCT 
GGCTCACTCT GTGCCCTGTT TTCTGATGCA GAATCGAGAT CCGAGATGAA GACTGAACTT 
TCTCCTCGGC CCGGGGCAGC AGGGCGGGAG TTGACCCAAG AAGAGAAGCT TCAGCTTCGA 
AAAGAAAAGA AACAGCAGAA GAAGAAACGG AAGGAGGAAA AGGGGGCAGA CCAAGAAATT 
GGCTCTGCTG TGTCTGCAGC TCAACGGCAA GACCCAATCA GAGAACTCCC AGGACCTGGT 
AGTCAGCTGG GAGGTACTGC TGGGGAGAAA CTTCCAGCTG GCCGGAGTAA GGCAGAACTT 
CGCGCCGAAA GGAGAGCCAA GCAGGAGGCA GAGCGGGCCC TGAAACAGGC AAGAAAAGGG 
GAACAAGGAG GCGTCCCTCC TCAGGCCTGC CCCAGCACTG CTGGAGAAAC CACCTCAGGA 
GTGAAGCGTG TCCCCGAGCA CACCCCAGCT GATGACCCCA CACTTCTGAG GAGGCTCCTT 
AGAAAGCCAG ATCGACAACA GGTTCCTACA CGAAAGGATT ACGGATCCAA AGTCAGTCTC 
TTCTCCCACC TGCCTCAGTA CAGCAGACAA AGCTCCTTGA CCCAGTACAT GAGCATCCCA 
TCCTCTGTGA TCCACCCAGC CATGGTGAGA CTCGGTCTGC AGTACTCCCA GGGCCTCATC 
AGTGGCTCCA ATGCCCGGTG CATCGCACTG CTTCACGCCC TGCAGCAGGT GATTCAGGAT 
TACACAACAC CTCCCAGTGA GGAACTCTCC AGGGATCTTG TAAATAAACT AAAACCCTAC 
ATCAGCTTCC TGACGCAGTG CCGCCCCATG TCGGCAAGTA TGTGCAACGC CATCAAGTTC 
CTAACCAAGG AAGTCACTGG CATGAGCAGC TCCAAGCGGG AAGAGGAGGC CAAGTCAGAA 
CTTAGAGAAG CCCTCGACCG GTACGTGCAA GAGAAGATCG TGCTTGCAGC TCAGGCAATT 
TCACGATTTG CTTCTACAAA GATCAGTGAT GGGGACGTGA TCCTGGTATA TGGATGCTCA 
TCTTTGGTGT CGAGAATTCT CCAGGAGGCC AGGGTCGAGG GCAGGCGGTT TCGGGTGGTG 
GTGGTGGACA GCCGGCCCCG GCTGGAAGGA AGGCACATGC TCCACTCTCT TGTCCGTGCT 
GGGGTCCCTA CCTCCTATCT GCTGATTCCT GCAGCCTCCT ATGTGCTTCC AGAGGTTTCT 
AAGGTGCTAT TGGGAGCTCA TGCACTCCTG GCCAATGGAT CTGTGATGTC TCGGGTAGGG 
ACAGCACAGT TGGCCCTGGT GGCTCGAGCT CATAACGTTC CAGTACTGGT CTGCTGTGAA 
ACGTACAAGT TCTGTGAACG TGTGCAGACT GATGCCTTTG TCTCCAACGA GCTAGATGAT 
CCCGATGATC TGCAGTGTAA GCGGGGAGAC CAGGTGGCCC TGGCTAACTG GCAGAGCCAC 
CCGTCCCTCC GGTTGTTGAA TCTGGTCTAT GACGTGACTC CACCTGAGCT CGTGGATCTG 
GTGATCACAG AGTTGGGCAT GATCCCTTGC AGTTCTGTGC CTGTTGTTCT TCGAGTCAAG 
AGCAGTGACC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001120827.1
CDS	202..1836
Translation	MPTQQPAAPTSLPKSSRSLSGSLCALFSDAESRSEMKTELSPRPGAAGRELTQEEKLQLRKEKKQQKKKRKEEKGADQEIGSAVSAAQRQDPIRELPGPGSQLGGTAGEKLPAGRSKAELRAERRAKQEAERALKQARKGEQGGVPPQACPSTAGETTSGVKRVPEHTPADDPTLLRRLLRKPDRQQVPTRKDYGSKVSLFSHLPQYSRQSSLTQYMSIPSSVIHPAMVRLGLQYSQGLISGSNARCIALLHALQQVIQDYTTPPSEELSRDLVNKLKPYISFLTQCRPMSASMCNAIKFLTKEVTGMSSSKREEEAKSELREALDRYVQEKIVLAAQAISRFASTKISDGDVILVYGCSSLVSRILQEARVEGRRFRVVVVDSRPRLEGRHMLHSLVRAGVPTSYLLIPAASYVLPEVSKVLLGAHALLANGSVMSRVGTAQLALVARAHNVPVLVCCETYKFCERVQTDAFVSNELDDPDDLQCKRGDQVALANWQSHPSLRLLNLVYDVTPPELVDLVITELGMIPCSSVPVVLRVKSSDQ

Target ORF information:

RefSeq Version	NM_001127355.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 2B, subunit 4 delta (Eif2b4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127355.1

ORF Insert Sequence:

ATGCCAACCC AGCAGCCAGC TGCGCCGACG AGTCTCCCCA AGTCCTCCCG GAGCCTGTCT 
GGCTCACTCT GTGCCCTGTT TTCTGATGCA GAATCGAGAT CCGAGATGAA GACTGAACTT 
TCTCCTCGGC CCGGGGCAGC AGGGCGGGAG TTGACCCAAG AAGAGAAGCT TCAGCTTCGA 
AAAGAAAAGA AACAGCAGAA GAAGAAACGG AAGGAGGAAA AGGGGGCAGA CCAAGAAATT 
GGCTCTGCTG TGTCTGCAGC TCAACGGCAA GACCCAATCA GAGAACTCCC AGGACCTGGT 
AGTCAGCTGG GAGGTACTGC TGGGGAGAAA CTTCCAGCTG GCCGGAGTAA GGCAGAACTT 
CGCGCCGAAA GGAGAGCCAA GCAGGAGGCA GAGCGGGCCC TGAAACAGGC AAGAAAAGGG 
GAACAAGGAG GCGTCCCTCC TCAGGCCTGC CCCAGCACTG CTGGAGAAAC CACCTCAGGA 
GTGAAGCGTG TCCCCGAGCA CACCCCAGCT GATGACCCCA CACTTCTGAG GAGGCTCCTT 
AGAAAGCCAG ATCGACAACA GGTTCCTACA CGAAAGGATT ACGGATCCAA AGTCAGTCTC 
TTCTCCCACC TGCCTCAGTA CAGCAGACAA AGCTCCTTGA CCCAGTACAT GAGCATCCCA 
TCCTCTGTGA TCCACCCAGC CATGGTGAGA CTCGGTCTGC AGTACTCCCA GGGCCTCATC 
AGTGGCTCCA ATGCCCGGTG CATCGCACTG CTTCACGCCC TGCAGCAGGT GATTCAGGAT 
TACACAACAC CTCCCAGTGA GGAACTCTCC AGGGATCTTG TAAATAAACT AAAACCCTAC 
ATCAGCTTCC TGACGCAGTG CCGCCCCATG TCGGCAAGTA TGTGCAACGC CATCAAGTTC 
CTAACCAAGG AAGTCACTGG CATGAGCAGC TCCAAGCGGG AAGAGGAGGC CAAGTCAGAA 
CTTAGAGAAG CCCTCGACCG GTACGTGCAA GAGAAGATCG TGCTTGCAGC TCAGGCAATT 
TCACGATTTG CTTCTACAAA GATCAGTGAT GGGGACGTGA TCCTGGTATA TGGATGCTCA 
TCTTTGGTGT CGAGAATTCT CCAGGAGGCC AGGGTCGAGG GCAGGCGGTT TCGGGTGGTG 
GTGGTGGACA GCCGGCCCCG GCTGGAAGGA AGGCACATGC TCCACTCTCT TGTCCGTGCT 
GGGGTCCCTA CCTCCTATCT GCTGATTCCT GCAGCCTCCT ATGTGCTTCC AGAGGTTTCT 
AAGGTGCTAT TGGGAGCTCA TGCACTCCTG GCCAATGGAT CTGTGATGTC TCGGGTAGGG 
ACAGCACAGT TGGCCCTGGT GGCTCGAGCT CATAACGTTC CAGTACTGGT CTGCTGTGAA 
ACGTACAAGT TCTGTGAACG TGTGCAGACT GATGCCTTTG TCTCCAACGA GCTAGATGAT 
CCCGATGATC TGCAGTGTAA GCGGGGAGAC CAGGTGGCCC TGGCTAACTG GCAGAGCCAC 
CCGTCCCTCC GGTTGTTGAA TCTGGTCTAT GACGTGACTC CACCTGAGCT CGTGGATCTG 
GTGATCACAG AGTTGGGCAT GATCCCTTGC AGTTCTGTGC CTGTTGTTCT TCGAGTCAAG 
AGCAGTGACC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eif2b4 ( NM_010122.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034252.2 Mus musculus eukaryotic translation initiation factor 2B, subunit 4 delta (Eif2b4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu05577
Clone ID Related Accession (Same CDS sequence)	NM_010122.2
Accession Version	NM_010122.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Mus musculus(house mouse)
Product	translation initiation factor eIF-2B subunit delta isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB665023.1, AK158282.1 and CD768292.1. On May 15, 2008 this sequence version replaced NM_010122.1. Transcript Variant: This variant (2) lacks a segment spanning the 5' UTR and 5' coding region, compared to variant 1, resulting in translation initiation from an alternate start codon and a shorter protein (isoform 2) with a distinct N-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: M98036.1, SRR1660817.284349.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCTGCGG TGGCGGTGGC TGTTCGCGAA GAATCGAGAT CCGAGATGAA GACTGAACTT 
TCTCCTCGGC CCGGGGCAGC AGGGCGGGAG TTGACCCAAG AAGAGAAGCT TCAGCTTCGA 
AAAGAAAAGA AACAGCAGAA GAAGAAACGG AAGGAGGAAA AGGGGGCAGA CCAAGAAATT 
GGCTCTGCTG TGTCTGCAGC TCAACGGCAA GACCCAATCA GAGAACTCCC AGGACCTGGT 
AGTCAGCTGG GAGGTACTGC TGGGGAGAAA CTTCCAGCTG GCCGGAGTAA GGCAGAACTT 
CGCGCCGAAA GGAGAGCCAA GCAGGAGGCA GAGCGGGCCC TGAAACAGGC AAGAAAAGGG 
GAACAAGGAG GCGTCCCTCC TCAGGCCTGC CCCAGCACTG CTGGAGAAAC CACCTCAGGA 
GTGAAGCGTG TCCCCGAGCA CACCCCAGCT GATGACCCCA CACTTCTGAG GAGGCTCCTT 
AGAAAGCCAG ATCGACAACA GGTTCCTACA CGAAAGGATT ACGGATCCAA AGTCAGTCTC 
TTCTCCCACC TGCCTCAGTA CAGCAGACAA AGCTCCTTGA CCCAGTACAT GAGCATCCCA 
TCCTCTGTGA TCCACCCAGC CATGGTGAGA CTCGGTCTGC AGTACTCCCA GGGCCTCATC 
AGTGGCTCCA ATGCCCGGTG CATCGCACTG CTTCACGCCC TGCAGCAGGT GATTCAGGAT 
TACACAACAC CTCCCAGTGA GGAACTCTCC AGGGATCTTG TAAATAAACT AAAACCCTAC 
ATCAGCTTCC TGACGCAGTG CCGCCCCATG TCGGCAAGTA TGTGCAACGC CATCAAGTTC 
CTAACCAAGG AAGTCACTGG CATGAGCAGC TCCAAGCGGG AAGAGGAGGC CAAGTCAGAA 
CTTAGAGAAG CCCTCGACCG GTACGTGCAA GAGAAGATCG TGCTTGCAGC TCAGGCAATT 
TCACGATTTG CTTCTACAAA GATCAGTGAT GGGGACGTGA TCCTGGTATA TGGATGCTCA 
TCTTTGGTGT CGAGAATTCT CCAGGAGGCC AGGGTCGAGG GCAGGCGGTT TCGGGTGGTG 
GTGGTGGACA GCCGGCCCCG GCTGGAAGGA AGGCACATGC TCCACTCTCT TGTCCGTGCT 
GGGGTCCCTA CCTCCTATCT GCTGATTCCT GCAGCCTCCT ATGTGCTTCC AGAGGTTTCT 
AAGGTGCTAT TGGGAGCTCA TGCACTCCTG GCCAATGGAT CTGTGATGTC TCGGGTAGGG 
ACAGCACAGT TGGCCCTGGT GGCTCGAGCT CATAACGTTC CAGTACTGGT CTGCTGTGAA 
ACGTACAAGT TCTGTGAACG TGTGCAGACT GATGCCTTTG TCTCCAACGA GCTAGATGAT 
CCCGATGATC TGCAGTGTAA GCGGGGAGAC CAGGTGGCCC TGGCTAACTG GCAGAGCCAC 
CCGTCCCTCC GGTTGTTGAA TCTGGTCTAT GACGTGACTC CACCTGAGCT CGTGGATCTG 
GTGATCACAG AGTTGGGCAT GATCCCTTGC AGTTCTGTGC CTGTTGTTCT TCGAGTCAAG 
AGCAGTGACC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034252.2
CDS	117..1691
Translation	MAAVAVAVREESRSEMKTELSPRPGAAGRELTQEEKLQLRKEKKQQKKKRKEEKGADQEIGSAVSAAQRQDPIRELPGPGSQLGGTAGEKLPAGRSKAELRAERRAKQEAERALKQARKGEQGGVPPQACPSTAGETTSGVKRVPEHTPADDPTLLRRLLRKPDRQQVPTRKDYGSKVSLFSHLPQYSRQSSLTQYMSIPSSVIHPAMVRLGLQYSQGLISGSNARCIALLHALQQVIQDYTTPPSEELSRDLVNKLKPYISFLTQCRPMSASMCNAIKFLTKEVTGMSSSKREEEAKSELREALDRYVQEKIVLAAQAISRFASTKISDGDVILVYGCSSLVSRILQEARVEGRRFRVVVVDSRPRLEGRHMLHSLVRAGVPTSYLLIPAASYVLPEVSKVLLGAHALLANGSVMSRVGTAQLALVARAHNVPVLVCCETYKFCERVQTDAFVSNELDDPDDLQCKRGDQVALANWQSHPSLRLLNLVYDVTPPELVDLVITELGMIPCSSVPVVLRVKSSDQ

Target ORF information:

RefSeq Version	NM_010122.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 2B, subunit 4 delta (Eif2b4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010122.2

ORF Insert Sequence:

ATGGCTGCGG TGGCGGTGGC TGTTCGCGAA GAATCGAGAT CCGAGATGAA GACTGAACTT 
TCTCCTCGGC CCGGGGCAGC AGGGCGGGAG TTGACCCAAG AAGAGAAGCT TCAGCTTCGA 
AAAGAAAAGA AACAGCAGAA GAAGAAACGG AAGGAGGAAA AGGGGGCAGA CCAAGAAATT 
GGCTCTGCTG TGTCTGCAGC TCAACGGCAA GACCCAATCA GAGAACTCCC AGGACCTGGT 
AGTCAGCTGG GAGGTACTGC TGGGGAGAAA CTTCCAGCTG GCCGGAGTAA GGCAGAACTT 
CGCGCCGAAA GGAGAGCCAA GCAGGAGGCA GAGCGGGCCC TGAAACAGGC AAGAAAAGGG 
GAACAAGGAG GCGTCCCTCC TCAGGCCTGC CCCAGCACTG CTGGAGAAAC CACCTCAGGA 
GTGAAGCGTG TCCCCGAGCA CACCCCAGCT GATGACCCCA CACTTCTGAG GAGGCTCCTT 
AGAAAGCCAG ATCGACAACA GGTTCCTACA CGAAAGGATT ACGGATCCAA AGTCAGTCTC 
TTCTCCCACC TGCCTCAGTA CAGCAGACAA AGCTCCTTGA CCCAGTACAT GAGCATCCCA 
TCCTCTGTGA TCCACCCAGC CATGGTGAGA CTCGGTCTGC AGTACTCCCA GGGCCTCATC 
AGTGGCTCCA ATGCCCGGTG CATCGCACTG CTTCACGCCC TGCAGCAGGT GATTCAGGAT 
TACACAACAC CTCCCAGTGA GGAACTCTCC AGGGATCTTG TAAATAAACT AAAACCCTAC 
ATCAGCTTCC TGACGCAGTG CCGCCCCATG TCGGCAAGTA TGTGCAACGC CATCAAGTTC 
CTAACCAAGG AAGTCACTGG CATGAGCAGC TCCAAGCGGG AAGAGGAGGC CAAGTCAGAA 
CTTAGAGAAG CCCTCGACCG GTACGTGCAA GAGAAGATCG TGCTTGCAGC TCAGGCAATT 
TCACGATTTG CTTCTACAAA GATCAGTGAT GGGGACGTGA TCCTGGTATA TGGATGCTCA 
TCTTTGGTGT CGAGAATTCT CCAGGAGGCC AGGGTCGAGG GCAGGCGGTT TCGGGTGGTG 
GTGGTGGACA GCCGGCCCCG GCTGGAAGGA AGGCACATGC TCCACTCTCT TGTCCGTGCT 
GGGGTCCCTA CCTCCTATCT GCTGATTCCT GCAGCCTCCT ATGTGCTTCC AGAGGTTTCT 
AAGGTGCTAT TGGGAGCTCA TGCACTCCTG GCCAATGGAT CTGTGATGTC TCGGGTAGGG 
ACAGCACAGT TGGCCCTGGT GGCTCGAGCT CATAACGTTC CAGTACTGGT CTGCTGTGAA 
ACGTACAAGT TCTGTGAACG TGTGCAGACT GATGCCTTTG TCTCCAACGA GCTAGATGAT 
CCCGATGATC TGCAGTGTAA GCGGGGAGAC CAGGTGGCCC TGGCTAACTG GCAGAGCCAC 
CCGTCCCTCC GGTTGTTGAA TCTGGTCTAT GACGTGACTC CACCTGAGCT CGTGGATCTG 
GTGATCACAG AGTTGGGCAT GATCCCTTGC AGTTCTGTGC CTGTTGTTCT TCGAGTCAAG 
AGCAGTGACC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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