Defa5 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Defa5
Entrez Gene ID	13239
Full Name	defensin, alpha, 5
Synonyms	Defa29,Defcr29,Defcr5
General protein information	Preferred Names defensin, alpha, 5 Names alpha-defensin 5 defensin, alpha, 29 defensin-related cryptdin-5
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 A2

mRNA and Protein(s)

mRNA	Protein	Name
NM_007851.2	NP_031877.2	alpha-defensin 5 precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Gm21002	NP_001257484.1
Mus musculus (house mouse)	Gm15293	NP_001170957.1
Mus musculus (house mouse)	Defa26	NP_001073402.1
Mus musculus (house mouse)	Defa3	NP_031876.1
Mus musculus (house mouse)	Defa23	NP_001012307.1
Mus musculus (house mouse)	Defa25	NP_031875.1
Mus musculus (house mouse)	Gm15315	NP_001170999.1
Mus musculus (house mouse)	Defa5	NP_031877.2
Mus musculus (house mouse)	Gm10104	NP_001170952.1
Mus musculus (house mouse)	Defa17	NP_001161262.1
Mus musculus (house mouse)	Gm15292	NP_001170958.1
Mus musculus (house mouse)	Defa20	NP_899091.2
Mus musculus (house mouse)	Defa2	NP_001182563.2
Mus musculus (house mouse)	Defa21	NP_899076.1
Mus musculus (house mouse)	Defa22	NP_997541.2
Mus musculus (house mouse)	Gm21498	NP_001257542.1
Mus musculus (house mouse)	Gm15299	NP_001164426.1
Mus musculus (house mouse)	Gm15284	NP_001170956.1
Mus musculus (house mouse)	Gm15308	NP_001170992.1
Mus musculus (house mouse)	Defa24	NP_001019396.1
Mus musculus (house mouse)	Defa-rs7	NP_031874.2
Mus musculus (house mouse)	Gm14850	NP_001170993.1
Mus musculus (house mouse)	Gm6696	NP_001170994.1

Gene Ontology
Bibliography

Related articles in PubMed

?-Defensin 5 gene expression is regulated by gut microbial metabolites.
Sugi Y, Takahashi K, Kurihara K, Nakano K, Kobayakawa T, Nakata K, Tsuda M, Hanazawa S, Hosono A, Kaminogawa S
Bioscience, biotechnology, and biochemistry81(2)242-248(2017 Feb)

Rab8a vesicles regulate Wnt ligand delivery and Paneth cell maturation at the intestinal stem cell niche.
Das S, Yu S, Sakamori R, Vedula P, Feng Q, Flores J, Hoffman A, Fu J, Stypulkowski E, Rodriguez A, Dobrowolski R, Harada A, Hsu W, Bonder EM, Verzi MP, Gao N
Development (Cambridge, England)142(12)2147-62(2015 Jun)

Enteric defensins are essential regulators of intestinal microbial ecology.
Salzman NH, Hung K, Haribhai D, Chu H, Karlsson-Sj?berg J, Amir E, Teggatz P, Barman M, Hayward M, Eastwood D, Stoel M, Zhou Y, Sodergren E, Weinstock GM, Bevins CL, Williams CB, Bos NA
Nature immunology11(1)76-83(2010 Jan)

Manual annotation and analysis of the defensin gene cluster in the C57BL/6J mouse reference genome.
Amid C, Rehaume LM, Brown KL, Gilbert JG, Dougan G, Hancock RE, Harrow JL
BMC genomics10606(2009 Dec)

LGR5 deficiency deregulates Wnt signaling and leads to precocious Paneth cell differentiation in the fetal intestine.
Garcia MI, Ghiani M, Lefort A, Libert F, Strollo S, Vassart G
Developmental biology331(1)58-67(2009 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

alpha-defensin 5 expression can also be regulated by microbial metabolites
Title: ?-Defensin 5 gene expression is regulated by gut microbial metabolites.

Data show that DEFA5-expressing mice had striking losses of segmented filamentous bacteria and fewer interleukin 17 (IL-17)-producing lamina propria T cells.
Title: Enteric defensins are essential regulators of intestinal microbial ecology.

The following Defa5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Defa5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu00915	NM_007851.2 Latest version!	Mus musculus defensin, alpha, 5 (Defa5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Defa5 ( NM_007851.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031877.2 Mus musculus defensin, alpha, 5 (Defa5), mRNA.

Price & Availability↑

CloneID	OMu00915
Clone ID Related Accession (Same CDS sequence)	NM_007851.2
Accession Version	NM_007851.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	alpha-defensin 5 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC129174.3, AV070515.1 and AV067547.1. On Aug 31, 2006 this sequence version replaced NM_007851.1. ##Evidence-Data-START## Transcript exon combination :: AV049261.1, AV070515.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849377 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGACAT TTGTCCTCCT CTCTGCCCTT GTCCTGCTGG CCTTCCAGGC CCAGGCTGAT 
CCTATCCACA AAACAGATGA AGAGACTAAT ACTGAGGAGC AGCCAGGGGA AGAGGACCAG 
GCTGTGTCTA TCTCCTTTGG AGGCCAAGAA GGGTCTGCTC TTCATGAAGA ATTGTCAAAA 
AAGCTGATAT GCTATTGTAG AATAAGAGGC TGCAAAAGAA GAGAACGCGT TTTTGGGACC 
TGCAGAAATC TTTTTTTAAC TTTCGTATTC TGCTGCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031877.2
CDS	46..327
Translation	MKTFVLLSALVLLAFQAQADPIHKTDEETNTEEQPGEEDQAVSISFGGQEGSALHEELSKKLICYCRIRGCKRRERVFGTCRNLFLTFVFCCS

Target ORF information:

RefSeq Version	NM_007851.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus defensin, alpha, 5 (Defa5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007851.2

ORF Insert Sequence:

ATGAAGACAT TTGTCCTCCT CTCTGCCCTT GTCCTGCTGG CCTTCCAGGC CCAGGCTGAT 
CCTATCCACA AAACAGATGA AGAGACTAAT ACTGAGGAGC AGCCAGGGGA AGAGGACCAG 
GCTGTGTCTA TCTCCTTTGG AGGCCAAGAA GGGTCTGCTC TTCATGAAGA ATTGTCAAAA 
AAGCTGATAT GCTATTGTAG AATAAGAGGC TGCAAAAGAA GAGAACGCGT TTTTGGGACC 
TGCAGAAATC TTTTTTTAAC TTTCGTATTC TGCTGCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

?-Defensin 5 gene expression is regulated by gut microbial metabolites.
Bioscience, biotechnology, and biochemistry81(2)242-248(2017 Feb)
Sugi Y,Takahashi K,Kurihara K,Nakano K,Kobayakawa T,Nakata K,Tsuda M,Hanazawa S,Hosono A,Kaminogawa S

Rab8a vesicles regulate Wnt ligand delivery and Paneth cell maturation at the intestinal stem cell niche.
Development (Cambridge, England)142(12)2147-62(2015 Jun)
Das S,Yu S,Sakamori R,Vedula P,Feng Q,Flores J,Hoffman A,Fu J,Stypulkowski E,Rodriguez A,Dobrowolski R,Harada A,Hsu W,Bonder EM,Verzi MP,Gao N

Enteric defensins are essential regulators of intestinal microbial ecology.
Nature immunology11(1)76-83(2010 Jan)
Salzman NH,Hung K,Haribhai D,Chu H,Karlsson-Sj?berg J,Amir E,Teggatz P,Barman M,Hayward M,Eastwood D,Stoel M,Zhou Y,Sodergren E,Weinstock GM,Bevins CL,Williams CB,Bos NA

Manual annotation and analysis of the defensin gene cluster in the C57BL/6J mouse reference genome.
BMC genomics10606(2009 Dec)
Amid C,Rehaume LM,Brown KL,Gilbert JG,Dougan G,Hancock RE,Harrow JL

LGR5 deficiency deregulates Wnt signaling and leads to precocious Paneth cell differentiation in the fetal intestine.
Developmental biology331(1)58-67(2009 Jul)
Garcia MI,Ghiani M,Lefort A,Libert F,Strollo S,Vassart G