Defa4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Defa4
Entrez Gene ID	13238
Full Name	defensin, alpha, 4
Synonyms	Crp4,Defa28,Defcr4
General protein information	Preferred Names defensin, alpha, 4 Names alpha-defensin 4 alpha-defensin, 28 defensin related cryptdin 4
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 A2

mRNA and Protein(s)

mRNA	Protein	Name
NM_010039.2	NP_034169.2	alpha-defensin 4 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Loss of Paneth Cell Autophagy Causes Acute Susceptibility to Toxoplasma gondii-Mediated Inflammation.
Burger E, Araujo A, L?pez-Yglesias A, Rajala MW, Geng L, Levine B, Hooper LV, Burstein E, Yarovinsky F
Cell host & microbe(2018 Jan)

Amplified pathogenic actions of angiotensin II in cysteine-rich LIM-only protein 4-negative mouse hearts.
Straubinger J, Boldt K, Kuret A, Deng L, Krattenmacher D, Bork N, Desch M, Feil R, Feil S, Nemer M, Ueffing M, Ruth P, Just S, Lukowski R
FASEB journal : official publication of the Federation of American Societies for Experimental Biology31(4)1620-1638(2017 04)

Biosynthesis and antimicrobial evaluation of backbone-cyclized ?-defensins.
Garcia AE, Tai KP, Puttamadappa SS, Shekhtman A, Ouellette AJ, Camarero JA
Biochemistry50(48)10508-19(2011 Dec)

Lgr4 is required for Paneth cell differentiation and maintenance of intestinal stem cells ex vivo.
Mustata RC, Van Loy T, Lefort A, Libert F, Strollo S, Vassart G, Garcia MI
EMBO reports12(6)558-64(2011 Jun)

Bactericidal activity of mouse ?-defensin cryptdin-4 predominantly affects noncommensal bacteria.
Masuda K, Sakai N, Nakamura K, Yoshioka S, Ayabe T
Journal of innate immunity3(3)315-26(2011)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Depleting CRP4 is incompatible with a proper cardiac development and function. CRP4 is a novel antiremodeling factor regulated, at least to some extent, by cardiac cGMP.
Title: Amplified pathogenic actions of angiotensin II in cysteine-rich LIM-only protein 4-negative mouse hearts.

Backbone cyclization provides a means for engineeringalpha-defensins to improve their biological activity while providing excellent stability to serum degradation and highlighting the potential of peptide scaffolds for development of novel compounds.
Title: Biosynthesis and antimicrobial evaluation of backbone-cyclized ?-defensins.

bactericidal activity predominantly affects noncommensal bacteria
Title: Bactericidal activity of mouse ?-defensin cryptdin-4 predominantly affects noncommensal bacteria.

Lys-for-Arg replacements in cryptdin 4 attenuated bactericidal activity
Title: Electropositive charge in alpha-defensin bactericidal activity: functional effects of Lys-for-Arg substitutions vary with the peptide primary structure.

Beta-sheet-containing Crp4 induces membrane permeabilization more effectively than disulfide-null Crp4 by translocating and forming transient membrane defects.
Title: Mechanisms of alpha-defensin bactericidal action: comparative membrane disruption by Cryptdin-4 and its disulfide-null analogue.

The following Defa4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Defa4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu67527	NM_010039.2 Latest version!	Mus musculus defensin, alpha, 4 (Defa4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Defa4 ( NM_010039.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034169.2 Mus musculus defensin, alpha, 4 (Defa4), mRNA.

Price & Availability↑

CloneID	OMu67527
Clone ID Related Accession (Same CDS sequence)	NM_010039.2
Accession Version	NM_010039.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Mus musculus(house mouse)
Product	alpha-defensin 4 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC134360.1 and BX634116.1. On Oct 11, 2014 this sequence version replaced NM_010039.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. COMPLETENESS: complete on the 3' end.

ATGAAGACAC TTGTCCTCCT CTCTGCCCTC GTCCTGCTGG CCTTCCAGGT CCAGGCTGAT 
CCTATCCAAA ACACAGATGA AGAGACTAAT ACTGAGGAGC AGCCAGGGGA AGAGGACCAG 
GCTGTGTCTA TCTCCTTTGG AGGCCAAGAA GGGTCTGCTC TTCATGAAAA ATCTTTGAGA 
GGTTTGTTAT GCTATTGTAG AAAAGGACAC TGCAAAAGAG GAGAACGAGT TCGTGGGACT 
TGTGGAATAC GATTTTTGTA CTGCTGCCCC CGCCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034169.2
CDS	1..279
Translation	MKTLVLLSALVLLAFQVQADPIQNTDEETNTEEQPGEEDQAVSISFGGQEGSALHEKSLRGLLCYCRKGHCKRGERVRGTCGIRFLYCCPRR

Target ORF information:

RefSeq Version	NM_010039.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus defensin, alpha, 4 (Defa4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010039.2

ORF Insert Sequence:

ATGAAGACAC TTGTCCTCCT CTCTGCCCTC GTCCTGCTGG CCTTCCAGGT CCAGGCTGAT 
CCTATCCAAA ACACAGATGA AGAGACTAAT ACTGAGGAGC AGCCAGGGGA AGAGGACCAG 
GCTGTGTCTA TCTCCTTTGG AGGCCAAGAA GGGTCTGCTC TTCATGAAAA ATCTTTGAGA 
GGTTTGTTAT GCTATTGTAG AAAAGGACAC TGCAAAAGAG GAGAACGAGT TCGTGGGACT 
TGTGGAATAC GATTTTTGTA CTGCTGCCCC CGCCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Loss of Paneth Cell Autophagy Causes Acute Susceptibility to Toxoplasma gondii-Mediated Inflammation.
Cell host & microbe(2018 Jan)
Burger E,Araujo A,L?pez-Yglesias A,Rajala MW,Geng L,Levine B,Hooper LV,Burstein E,Yarovinsky F

Amplified pathogenic actions of angiotensin II in cysteine-rich LIM-only protein 4-negative mouse hearts.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology31(4)1620-1638(2017 04)
Straubinger J,Boldt K,Kuret A,Deng L,Krattenmacher D,Bork N,Desch M,Feil R,Feil S,Nemer M,Ueffing M,Ruth P,Just S,Lukowski R

Biosynthesis and antimicrobial evaluation of backbone-cyclized ?-defensins.
Biochemistry50(48)10508-19(2011 Dec)
Garcia AE,Tai KP,Puttamadappa SS,Shekhtman A,Ouellette AJ,Camarero JA

Lgr4 is required for Paneth cell differentiation and maintenance of intestinal stem cells ex vivo.
EMBO reports12(6)558-64(2011 Jun)
Mustata RC,Van Loy T,Lefort A,Libert F,Strollo S,Vassart G,Garcia MI

Bactericidal activity of mouse ?-defensin cryptdin-4 predominantly affects noncommensal bacteria.
Journal of innate immunity3(3)315-26(2011)
Masuda K,Sakai N,Nakamura K,Yoshioka S,Ayabe T