Defb1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Defb1
Entrez Gene ID	13214
Full Name	defensin beta 1
Synonyms	AW260221,BD-1
General protein information	Preferred Names defensin beta 1 Names beta-defensin 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8 A2\|8 10.35 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_007843.3	NP_031869.1	beta-defensin 1 precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Defb1	NP_031869.1
Rattus norvegicus (Norway rat)	Defb1	NP_113998.1

Gene Ontology
Bibliography

Related articles in PubMed

Cutaneous Nod2 Expression Regulates the?Skin Microbiome and Wound Healing in?a Murine Model.
Williams H, Crompton RA, Thomason HA, Campbell L, Singh G, McBain AJ, Cruickshank SM, Hardman MJ
The Journal of investigative dermatology137(11)2427-2436(2017 Nov)

Defective glucocorticoid receptor signaling and keratinocyte-autonomous defects contribute to skin phenotype of mouse embryos lacking the Hsp90 co-chaperone p23.
Madon-Simon M, Grad I, Bayo P, P?rez P, Picard D
PloS one12(6)e0180035(2017)

Differential Roles for Interleukin-23 and Interleukin-17 in Intestinal Immunoregulation.
Maxwell JR, Zhang Y, Brown WA, Smith CL, Byrne FR, Fiorino M, Stevens E, Bigler J, Davis JA, Rottman JB, Budelsky AL, Symons A, Towne JE
Immunity43(4)739-50(2015 Oct)

Angiogenesis QTL on Mouse Chromosome 8 Colocalizes with Differential ?-Defensin Expression.
Smith J, Liu F, Beyer B, Morales K, Reilly A, Cole R, Herron BJ
Journal of biomolecular techniques : JBT26(2)45-53(2015 Jul)

PPAR? agonists inhibit inflammatory activation of macrophages through upregulation of ?-defensin 1.
Ann SJ, Chung JH, Park BH, Kim SH, Jang J, Park S, Kang SM, Lee SH
Atherosclerosis240(2)389-97(2015 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Fenofibrate and gemfibrozil inhibit LPS-induced inflammatory activation of macrophages via PPARalpha/beta-defensin 1/TLR4 pathway.
Title: PPAR? agonists inhibit inflammatory activation of macrophages through upregulation of ?-defensin 1.

these studies demonstrate a role for the murine beta-defensin 1 peptide in early control of Candida infection in a murine model of mucosal candidiasis
Title: ?-Defensin 1 plays a role in acute mucosal defense against Candida albicans.

Histopathology showed a greater inflammatory influx in the lungs of mBD-1((-/-)) mice at Day 3 postinfection compared with WT C57BL/6 mice
Title: Modulation of human beta-defensin-1 (hBD-1) in plasmacytoid dendritic cells (PDC), monocytes, and epithelial cells by influenza virus, Herpes simplex virus, and Sendai virus and its possible role in innate immunity.

Akt1 is involved in the regulation of defensin expression and the innate immune response important for bacterial clearance
Title: Anti-bacterial effects of poly-N-acetyl-glucosamine nanofibers in cutaneous wound healing: requirement for Akt1.

The decreased production of antimicrobial peptides by burn-site epidermal keratinocytes influenced by Gr-1(+)CD11b(+) cells was shown to be restored by glycyrrhizin.
Title: Pivotal advance: glycyrrhizin restores the impaired production of beta-defensins in tissues surrounding the burn area and improves the resistance of burn mice to Pseudomonas aeruginosa wound infection.

The following Defb1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Defb1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu00901	NM_007843.3 Latest version!	Mus musculus defensin beta 1 (Defb1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Defb1 ( NM_007843.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031869.1 Mus musculus defensin beta 1 (Defb1), mRNA.

Price & Availability↑

CloneID	OMu00901
Clone ID Related Accession (Same CDS sequence)	NM_007843.3
Accession Version	NM_007843.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	beta-defensin 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AI956736.1, AK142544.1 and DV551556.1. On Jan 10, 2008 this sequence version replaced NM_007843.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC024380.1, DV055613.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAAACTC ATTACTTTCT CCTGGTGATG ATATGTTTTC TTTTCTCCCA GATGGAGCCA 
GGTGTTGGCA TTCTCACAAG TCTTGGACGA AGAACAGATC AATACAAATG CCTTCAACAT 
GGAGGATTCT GTCTCCGCTC CAGCTGCCCA TCTAATACCA AACTACAGGG AACCTGTAAA 
CCAGATAAGC CCAACTGTTG TAAGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031869.1
CDS	98..307
Translation	MKTHYFLLVMICFLFSQMEPGVGILTSLGRRTDQYKCLQHGGFCLRSSCPSNTKLQGTCKPDKPNCCKS

Target ORF information:

RefSeq Version	NM_007843.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus defensin beta 1 (Defb1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007843.3

ORF Insert Sequence:

ATGAAAACTC ATTACTTTCT CCTGGTGATG ATATGTTTTC TTTTCTCCCA GATGGAGCCA 
GGTGTTGGCA TTCTCACAAG TCTTGGACGA AGAACAGATC AATACAAATG CCTTCAACAT 
GGAGGATTCT GTCTCCGCTC CAGCTGCCCA TCTAATACCA AACTACAGGG AACCTGTAAA 
CCAGATAAGC CCAACTGTTG TAAGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cutaneous Nod2 Expression Regulates the?Skin Microbiome and Wound Healing in?a Murine Model.
The Journal of investigative dermatology137(11)2427-2436(2017 Nov)
Williams H,Crompton RA,Thomason HA,Campbell L,Singh G,McBain AJ,Cruickshank SM,Hardman MJ

Defective glucocorticoid receptor signaling and keratinocyte-autonomous defects contribute to skin phenotype of mouse embryos lacking the Hsp90 co-chaperone p23.
PloS one12(6)e0180035(2017)
Madon-Simon M,Grad I,Bayo P,P?rez P,Picard D

Differential Roles for Interleukin-23 and Interleukin-17 in Intestinal Immunoregulation.
Immunity43(4)739-50(2015 Oct)
Maxwell JR,Zhang Y,Brown WA,Smith CL,Byrne FR,Fiorino M,Stevens E,Bigler J,Davis JA,Rottman JB,Budelsky AL,Symons A,Towne JE

Angiogenesis QTL on Mouse Chromosome 8 Colocalizes with Differential ?-Defensin Expression.
Journal of biomolecular techniques : JBT26(2)45-53(2015 Jul)
Smith J,Liu F,Beyer B,Morales K,Reilly A,Cole R,Herron BJ

PPAR? agonists inhibit inflammatory activation of macrophages through upregulation of ?-defensin 1.
Atherosclerosis240(2)389-97(2015 Jun)
Ann SJ,Chung JH,Park BH,Kim SH,Jang J,Park S,Kang SM,Lee SH