Cdkn2c cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cdkn2c
Entrez Gene ID	12580
Full Name	cyclin-dependent kinase inhibitor 2C (p18, inhibits CDK4)
Synonyms	C77269,INK4c,p18,p18-INK4c,p18-INK6,p18INK4c
General protein information	Preferred Names cyclin-dependent kinase inhibitor 2C (p18, inhibits CDK4) Names cyclin-dependent kinase 4 inhibitor C cyclin-dependent kinase 6 inhibitor
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4 C7\|4 51.32 cM
Summary	The protein encoded by this gene is a member of the INK4 family of cyclin-dependent kinase (cdk) inhibitors, and contains five ankyrin repeats. This protein interacts with both Cdk4 and Cdk6 to inhibit their kinase activities, and prevent their interactions with D-type cyclins, thereby negatively regulating cell division. This gene is differentially expressed in a variety of tissues, and is cell cycle regulated. Deletion of this gene can lead to tumor growth. Maximal expression is observed at the G2/M phase. Alternative splicing and promoter usage results in multiple transript variants. [provided by RefSeq, Aug 2014].

mRNA and Protein(s)

mRNA	Protein	Name
NM_007671.3	NP_031697.1	cyclin-dependent kinase 4 inhibitor C
NM_001301368.1	NP_001288297.1	cyclin-dependent kinase 4 inhibitor C

Pathways from BioSystems

Homologies

	CDKN2C	XP_001110979.1
Bos taurus (cattle)	CDKN2C	NP_001094524.1
Rattus norvegicus (Norway rat)	Cdkn2c	NP_571977.1
Gallus gallus (chicken)	CDKN2C	XP_004936837.1
Homo sapiens (human)	CDKN2C	NP_001253.1
Pan troglodytes (chimpanzee)	CDKN2C	XP_524704.2
Canis lupus familiaris (dog)	CDKN2C	XP_849772.1
Mus musculus (house mouse)	Cdkn2c	NP_031697.1
Danio rerio (zebrafish)	cdkn2c	NP_001186923.1
Xenopus tropicalis (tropical clawed frog)	cdkn2c	NP_001116932.1

Gene Ontology
Bibliography

Related articles in PubMed

Host Tumor Suppressor p18
Niemeyer BF, Oko LM, Medina EM, Oldenburg DG, White DW, Cool CD, Clambey ET, van Dyk LF
Journal of virology(2018 Jan)

mTORC1-Mediated Inhibition of 4EBP1 Is Essential for Hedgehog Signaling-Driven Translation and Medulloblastoma.
Wu CC, Hou S, Orr BA, Kuo BR, Youn YH, Ong T, Roth F, Eberhart CG, Robinson GW, Solecki DJ, Taketo MM, Gilbertson RJ, Roussel MF, Han YG
Developmental cell43(6)673-688.e5(2017 Dec)

Cell cycle-targeting microRNAs promote differentiation by enforcing cell-cycle exit.
Otto T, Candido SV, Pilarz MS, Sicinska E, Bronson RT, Bowden M, Lachowicz IA, Mulry K, Fassl A, Han RC, Jecrois ES, Sicinski P
Proceedings of the National Academy of Sciences of the United States of America114(40)10660-10665(2017 Oct)

Coupled transcriptional and translational control of cyclin-dependent kinase inhibitor p18INK4c expression during myogenesis.
Phelps DE, Hsiao KM, Li Y, Hu N, Franklin DS, Westphal E, Lee EY, Xiong Y
Molecular and cellular biology18(4)2334-43(1998 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

p18 represents a potent and specific target for enhancing the self-renewal property of HSC, as demonstrated the effect of inhibitors
Title: Small-molecule inhibitors targeting INK4 protein p18(INK4C) enhance ex vivo expansion of haematopoietic stem cells.

Cdk4 and Cdk6 cooperate in hematopoietic tumor development and suggest a role for Cdk6 in sequestering INK4 proteins away from Cdk4.
Title: Cdk4 and Cdk6 cooperate in counteracting the INK4 family of inhibitors during murine leukemogenesis.

p18 may play protective roles and may be associated with or partially account for the cytoprotective effects of HO-1 in cisplatin-induced acute kidney injury.
Title: Cyclin-dependent kinase inhibitor p18INK4c is involved in protective roles of heme oxygenase-1 in cisplatin-induced acute kidney injury.

CD4+ T cells from mice genetically deficient for p18ink4c divide more rapidly than wild-type cells in response to antigenic, costimulatory and growth factor signals.
Title: Regulation of T cell differentiation and alloimmunity by the cyclin-dependent kinase inhibitor p18ink4c.

Gata3/Ruvbl2 complex regulates T helper 2 cell proliferation via repression of Cdkn2c expression.
Title: Gata3/Ruvbl2 complex regulates T helper 2 cell proliferation via repression of Cdkn2c expression.

The following Cdkn2c gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cdkn2c cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu22051	NM_001301368.1 Latest version!	Mus musculus cyclin dependent kinase inhibitor 2C (Cdkn2c), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu22051	NM_007671.3 Latest version!	Mus musculus cyclin dependent kinase inhibitor 2C (Cdkn2c), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cdkn2c ( NM_007671.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031697.1 Mus musculus cyclin dependent kinase inhibitor 2C (Cdkn2c), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu22051
Clone ID Related Accession (Same CDS sequence)	NM_007671.3 , NM_001301368.1
Accession Version	NM_007671.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 507bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	cyclin-dependent kinase 4 inhibitor C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL627392.12, AK003933.1, BF657811.1 and AA189303.1. On Aug 15, 2014 this sequence version replaced NM_007671.2. Transcript Variant: This variant (2, also known as p18(S)) differs in the 5' UTR and represents use of an alternate promoter, compared to variant 1. Both variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC027026.1, AK003933.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGAGC CTTGGGGGAA CGAGTTGGCG TCCGCAGCTG CCAGGGGGGA CCTAGAGCAA 
CTTACTAGTT TGTTGCAAAA TAATGTAAAC GTCAACGCTC AAAATGGATT TGGGAGAACT 
GCGCTGCAGG TTATGAAACT TGGAAATCCG GAGATTGCCA GGAGGCTTCT CCTCAGAGGT 
GCTAATCCCA ATTTGAAAGA TGGAACTGGT TTTGCTGTCA TTCATGATGC TGCCAGAGCA 
GGTTTCCTGG ACACTGTACA GGCTTTGCTG GAGTTCCAGG CTGATGTTAA CATTGAAGAT 
AATGAAGGGA ACCTGCCCTT GCACTTGGCT GCCAAAGAAG GCCACCTCCC TGTGGTGGAG 
TTCCTTATGA AGCACACAGC CTGCAATGTG GGGCATCGGA ACCATAAGGG GGACACCGCC 
TTCGACTTGG CCAGGTTCTA TGGAAGAAAT GAGGTCATTA GCCTGATGGA GGCAAATGGG 
GTTGGGGGAG CCACAAGCCT GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031697.1
CDS	227..733
Translation	MAEPWGNELASAAARGDLEQLTSLLQNNVNVNAQNGFGRTALQVMKLGNPEIARRLLLRGANPNLKDGTGFAVIHDAARAGFLDTVQALLEFQADVNIEDNEGNLPLHLAAKEGHLPVVEFLMKHTACNVGHRNHKGDTAFDLARFYGRNEVISLMEANGVGGATSLQ

Target ORF information:

RefSeq Version	NM_007671.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus cyclin dependent kinase inhibitor 2C (Cdkn2c), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007671.3

ORF Insert Sequence:

ATGGCCGAGC CTTGGGGGAA CGAGTTGGCG TCCGCAGCTG CCAGGGGGGA CCTAGAGCAA 
CTTACTAGTT TGTTGCAAAA TAATGTAAAC GTCAACGCTC AAAATGGATT TGGGAGAACT 
GCGCTGCAGG TTATGAAACT TGGAAATCCG GAGATTGCCA GGAGGCTTCT CCTCAGAGGT 
GCTAATCCCA ATTTGAAAGA TGGAACTGGT TTTGCTGTCA TTCATGATGC TGCCAGAGCA 
GGTTTCCTGG ACACTGTACA GGCTTTGCTG GAGTTCCAGG CTGATGTTAA CATTGAAGAT 
AATGAAGGGA ACCTGCCCTT GCACTTGGCT GCCAAAGAAG GCCACCTCCC TGTGGTGGAG 
TTCCTTATGA AGCACACAGC CTGCAATGTG GGGCATCGGA ACCATAAGGG GGACACCGCC 
TTCGACTTGG CCAGGTTCTA TGGAAGAAAT GAGGTCATTA GCCTGATGGA GGCAAATGGG 
GTTGGGGGAG CCACAAGCCT GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdkn2c ( NM_001301368.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001288297.1 Mus musculus cyclin dependent kinase inhibitor 2C (Cdkn2c), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu22051
Clone ID Related Accession (Same CDS sequence)	NM_007671.3 , NM_001301368.1
Accession Version	NM_001301368.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 507bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	cyclin-dependent kinase 4 inhibitor C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL627392.12, BG085472.2, AK003933.1 and AA189303.1. On Aug 15, 2014 this sequence version replaced XM_006502698.1. Transcript Variant: This variant (1, also known as p18(L)) represents the longer transcript. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK157209.1, U19596.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCCGAGC CTTGGGGGAA CGAGTTGGCG TCCGCAGCTG CCAGGGGGGA CCTAGAGCAA 
CTTACTAGTT TGTTGCAAAA TAATGTAAAC GTCAACGCTC AAAATGGATT TGGGAGAACT 
GCGCTGCAGG TTATGAAACT TGGAAATCCG GAGATTGCCA GGAGGCTTCT CCTCAGAGGT 
GCTAATCCCA ATTTGAAAGA TGGAACTGGT TTTGCTGTCA TTCATGATGC TGCCAGAGCA 
GGTTTCCTGG ACACTGTACA GGCTTTGCTG GAGTTCCAGG CTGATGTTAA CATTGAAGAT 
AATGAAGGGA ACCTGCCCTT GCACTTGGCT GCCAAAGAAG GCCACCTCCC TGTGGTGGAG 
TTCCTTATGA AGCACACAGC CTGCAATGTG GGGCATCGGA ACCATAAGGG GGACACCGCC 
TTCGACTTGG CCAGGTTCTA TGGAAGAAAT GAGGTCATTA GCCTGATGGA GGCAAATGGG 
GTTGGGGGAG CCACAAGCCT GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001288297.1
CDS	1159..1665
Translation	MAEPWGNELASAAARGDLEQLTSLLQNNVNVNAQNGFGRTALQVMKLGNPEIARRLLLRGANPNLKDGTGFAVIHDAARAGFLDTVQALLEFQADVNIEDNEGNLPLHLAAKEGHLPVVEFLMKHTACNVGHRNHKGDTAFDLARFYGRNEVISLMEANGVGGATSLQ

Target ORF information:

RefSeq Version	NM_001301368.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cyclin dependent kinase inhibitor 2C (Cdkn2c), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301368.1

ORF Insert Sequence:

ATGGCCGAGC CTTGGGGGAA CGAGTTGGCG TCCGCAGCTG CCAGGGGGGA CCTAGAGCAA 
CTTACTAGTT TGTTGCAAAA TAATGTAAAC GTCAACGCTC AAAATGGATT TGGGAGAACT 
GCGCTGCAGG TTATGAAACT TGGAAATCCG GAGATTGCCA GGAGGCTTCT CCTCAGAGGT 
GCTAATCCCA ATTTGAAAGA TGGAACTGGT TTTGCTGTCA TTCATGATGC TGCCAGAGCA 
GGTTTCCTGG ACACTGTACA GGCTTTGCTG GAGTTCCAGG CTGATGTTAA CATTGAAGAT 
AATGAAGGGA ACCTGCCCTT GCACTTGGCT GCCAAAGAAG GCCACCTCCC TGTGGTGGAG 
TTCCTTATGA AGCACACAGC CTGCAATGTG GGGCATCGGA ACCATAAGGG GGACACCGCC 
TTCGACTTGG CCAGGTTCTA TGGAAGAAAT GAGGTCATTA GCCTGATGGA GGCAAATGGG 
GTTGGGGGAG CCACAAGCCT GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Host Tumor Suppressor p18
Journal of virology(2018 Jan)
Niemeyer BF,Oko LM,Medina EM,Oldenburg DG,White DW,Cool CD,Clambey ET,van Dyk LF

mTORC1-Mediated Inhibition of 4EBP1 Is Essential for Hedgehog Signaling-Driven Translation and Medulloblastoma.
Developmental cell43(6)673-688.e5(2017 Dec)
Wu CC,Hou S,Orr BA,Kuo BR,Youn YH,Ong T,Roth F,Eberhart CG,Robinson GW,Solecki DJ,Taketo MM,Gilbertson RJ,Roussel MF,Han YG

Cell cycle-targeting microRNAs promote differentiation by enforcing cell-cycle exit.
Proceedings of the National Academy of Sciences of the United States of America114(40)10660-10665(2017 Oct)
Otto T,Candido SV,Pilarz MS,Sicinska E,Bronson RT,Bowden M,Lachowicz IA,Mulry K,Fassl A,Han RC,Jecrois ES,Sicinski P

Coupled transcriptional and translational control of cyclin-dependent kinase inhibitor p18INK4c expression during myogenesis.
Molecular and cellular biology18(4)2334-43(1998 Apr)
Phelps DE,Hsiao KM,Li Y,Hu N,Franklin DS,Westphal E,Lee EY,Xiong Y