Cdkn2b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cdkn2b
Entrez Gene ID	12579
Full Name	cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4)
Synonyms	AV083695,INK4b,MTS2,p15,p15(INK4b),p15INK4b
General protein information	Preferred Names cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4) Names cyclin-dependent kinase 4 inhibitor B cyclin-dependent kinase inhibitor p15 cyclin-dependent kinase inhibitor p15INK4b cyclin-dependent kinase inhibitor protein p14-INK4b p15-INK4b
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4 C4\|4 42.15 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_007670.4	NP_031696.1	cyclin-dependent kinase 4 inhibitor B

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CDKN2B	NP_001139741.1
Mus musculus (house mouse)	Cdkn2b	NP_031696.1
Rattus norvegicus (Norway rat)	Cdkn2b	NP_570825.1
Bos taurus (cattle)	CDKN2B	NP_001069362.1
Homo sapiens (human)	CDKN2B	NP_004927.2
Pan troglodytes (chimpanzee)	CDKN2B	XP_003312069.1
	CDKN2B	XP_001107263.1
Gallus gallus (chicken)	CDKN2B	NP_989764.1

Gene Ontology
Bibliography

Related articles in PubMed

CDKN2B deletion is essential for pancreatic cancer development instead of unmeaningful co-deletion due to juxtaposition to CDKN2A.
Tu Q, Hao J, Zhou X, Yan L, Dai H, Sun B, Yang D, An S, Lv L, Jiao B, Chen C, Lai R, Shi P, Zhao X
Oncogene37(1)128-138(2018 Jan)

Histone deacetylase 1 and 2 are essential for murine neural crest proliferation, pharyngeal arch development, and craniofacial morphogenesis.
Milstone ZJ, Lawson G, Trivedi CM
Developmental dynamics : an official publication of the American Association of Anatomists246(12)1015-1026(2017 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that the Wnt-effector hepatocyte nuclear factor 1-alpha (Tcf1) is recruited to and triggers transcription of the Ink4/Arf tumor suppressor locus, such as as p15Ink4b, p16Ink4a and p19Arf.
Title: Wnt/Tcf1 pathway restricts embryonic stem cell cycle through activation of the Ink4/Arf locus.

The expression of three tumor suppressor genes encoded in the INK4/ARF locus (p15(INK4b), p16(INK4a), and p19(ARF)) was decreased in E6AP(-/-) embryo fibroblasts.
Title: Reduced abundance of the E3 ubiquitin ligase E6AP contributes to decreased expression of the INK4/ARF locus in non-small cell lung cancer.

miR-541 contributes to microcystin-LR-induced testicular toxicity by regulating the expression of p15 and promoting apoptosis.
Title: miR-541 Contributes to Microcystin-LR-Induced Reproductive Toxicity through Regulating the Expression of p15 in Mice.

Loss of CDKN2B may not only promote cardiovascular disease through the development of atherosclerosis but may also impair TGFbeta signaling and hypoxic neovessel maturation.
Title: CDKN2B Regulates TGF? Signaling and Smooth Muscle Cell Investment of Hypoxic Neovessels.

Loss of Nf2 and Cdkn2a/b have synergistic effects with PDGF-B overexpression promoting meningioma malignant transformation.
Title: PDGF activation in PGDS-positive arachnoid cells induces meningioma formation in mice promoting tumor progression in combination with Nf2 and Cdkn2ab loss.

The following Cdkn2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cdkn2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu20576	NM_007670.4 Latest version!	Mus musculus cyclin dependent kinase inhibitor 2B (Cdkn2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cdkn2b ( NM_007670.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031696.1 Mus musculus cyclin dependent kinase inhibitor 2B (Cdkn2b), mRNA.

Price & Availability↑

CloneID	OMu20576
Clone ID Related Accession (Same CDS sequence)	NM_007670.4
Accession Version	NM_007670.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	cyclin-dependent kinase 4 inhibitor B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CAAA01036593.1, AF059567.1 and DT919921.1. On Dec 4, 2008 this sequence version replaced NM_007670.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF059567.1, AK144132.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGTTGGGCG GCAGCAGTGA CGCGGGCCTG GCCACCGCCG CGGCGCGGGG GCAAGTGGAG 
ACGGTGCGGC AGCTCCTGGA AGCCGGCGCA GATCCCAACG CCCTGAACCG CTTCGGGAGG 
CGCCCAATCC AGGTCATGAT GATGGGCAGC GCCCAGGTGG CAGAGCTGCT GCTGCTCCAC 
GGAGCAGAAC CCAACTGCGC CGACCCTGCC ACCCTTACCA GACCTGTGCA CGACGCAGCT 
CGGGAAGGCT TCCTGGACAC GCTTGTCGTG CTGCACCGGG CAGGGGCGCG GCTGGATGTG 
TGTGACGCCT GGGGCCGCCT GCCGGTAGAC TTGGCTGAAG AGCAGGGCCA CCGTGACATT 
GCGAGGTATC TGCACGCTGC CACTGGAGAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031696.1
CDS	236..628
Translation	MLGGSSDAGLATAAARGQVETVRQLLEAGADPNALNRFGRRPIQVMMMGSAQVAELLLLHGAEPNCADPATLTRPVHDAAREGFLDTLVVLHRAGARLDVCDAWGRLPVDLAEEQGHRDIARYLHAATGD

Target ORF information:

RefSeq Version	NM_007670.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus cyclin dependent kinase inhibitor 2B (Cdkn2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007670.4

ORF Insert Sequence:

ATGTTGGGCG GCAGCAGTGA CGCGGGCCTG GCCACCGCCG CGGCGCGGGG GCAAGTGGAG 
ACGGTGCGGC AGCTCCTGGA AGCCGGCGCA GATCCCAACG CCCTGAACCG CTTCGGGAGG 
CGCCCAATCC AGGTCATGAT GATGGGCAGC GCCCAGGTGG CAGAGCTGCT GCTGCTCCAC 
GGAGCAGAAC CCAACTGCGC CGACCCTGCC ACCCTTACCA GACCTGTGCA CGACGCAGCT 
CGGGAAGGCT TCCTGGACAC GCTTGTCGTG CTGCACCGGG CAGGGGCGCG GCTGGATGTG 
TGTGACGCCT GGGGCCGCCT GCCGGTAGAC TTGGCTGAAG AGCAGGGCCA CCGTGACATT 
GCGAGGTATC TGCACGCTGC CACTGGAGAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CDKN2B deletion is essential for pancreatic cancer development instead of unmeaningful co-deletion due to juxtaposition to CDKN2A.
Oncogene37(1)128-138(2018 Jan)
Tu Q,Hao J,Zhou X,Yan L,Dai H,Sun B,Yang D,An S,Lv L,Jiao B,Chen C,Lai R,Shi P,Zhao X

Histone deacetylase 1 and 2 are essential for murine neural crest proliferation, pharyngeal arch development, and craniofacial morphogenesis.
Developmental dynamics : an official publication of the American Association of Anatomists246(12)1015-1026(2017 Dec)
Milstone ZJ,Lawson G,Trivedi CM