Cdc25b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cdc25b
Entrez Gene ID	12531
Full Name	cell division cycle 25B
Synonyms	AI604853
General protein information	Preferred Names cell division cycle 25B Names M-phase inducer phosphatase 2 dual specificity phosphatase Cdc25B
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 F1\|2 63.29 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_023117.4	NP_075606.1	M-phase inducer phosphatase 2 isoform a
NM_001111075.4	NP_001104545.1	M-phase inducer phosphatase 2 isoform b

Pathways from BioSystems

Homologies

Homo sapiens (human)	CDC25B	NP_068659.1
Bos taurus (cattle)	CDC25B	NP_001178344.1
Pan troglodytes (chimpanzee)	CDC25B	XP_001162751.1
Mus musculus (house mouse)	Cdc25b	NP_075606.1
Rattus norvegicus (Norway rat)	Cdc25b	NP_598256.1
Xenopus tropicalis (tropical clawed frog)	cdc25b	XP_002936339.1
Canis lupus familiaris (dog)	CDC25B	XP_850186.1

Gene Ontology
Bibliography

Related articles in PubMed

APC/C
Rattani A, Ballesteros Mejia R, Roberts K, Roig MB, Godwin J, Hopkins M, Eguren M, Sanchez-Pulido L, Okaz E, Ogushi S, Wolna M, Metson J, Pend?s AM, Malumbres M, Nov?k B, Herbert M, Nasmyth K
Current biology : CB27(10)1462-1476.e5(2017 May)

CDC25B is involved in the centrosomal microtubule nucleation in two-cell stage mouse embryos.
Cui C, Zang T, Cao Y, Qin X, Zhang X
Development, growth & differentiation58(9)714-726(2016 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

data suggest an important role of CDC25B for microtubule nucleation and organization. N-terminal of CDC25B is required for regulating the microtubule dynamics and mitotic function.
Title: CDC25B is involved in the centrosomal microtubule nucleation in two-cell stage mouse embryos.

LSD1 is essential for oocyte meiotic progression by upregulating CDC25B expression.
Title: LSD1 is essential for oocyte meiotic progression by regulating CDC25B expression in mice.

AURKA induced phosphorylation and recruitment of CDC25B to MTOCs prior to p-Cyclin B1-Ser123, and this sequential regulation is essential for the commitment of the oocytes to resume meiosis.
Title: Successive recruitment of p-CDC25B-Ser351 and p-cyclin B1-Ser123 to centrosomes contributes to the release of mouse oocytes from prophase I arrest.

The role of Cdc25c and Cdc25b in activating G2/M cell cycle checkpoint in zygote.
Title: Phospho-Cdc25 correlates with activating G2/M checkpoint in mouse zygotes fertilized with hydrogen peroxide-treated mouse sperm.

Data indicate that 14-3-3epsilon is required for the mitotic entry in the fertilized mouse eggs and responsible for sequestering the CDC25B in cytoplasm. Its binding to CDC25B-S321 phosphorylated by PKA induces mitotic arrest.
Title: 14-3-3 epsilon prevents G2/M transition of fertilized mouse eggs by binding with CDC25B.

The following Cdc25b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cdc25b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu19975	NM_001111075.4 Latest version!	Mus musculus cell division cycle 25B (Cdc25b), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu20140	NM_023117.4 Latest version!	Mus musculus cell division cycle 25B (Cdc25b), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cdc25b ( NM_001111075.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001104545.1 Mus musculus cell division cycle 25B (Cdc25b), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu19975
Clone ID Related Accession (Same CDS sequence)	NM_001111075.4
Accession Version	NM_001111075.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1653bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	M-phase inducer phosphatase 2 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL831736.16, BC057568.1 and AL808128.4. On Mar 21, 2012 this sequence version replaced NM_001111075.3. Transcript Variant: This variant (2) lacks an alternate in-frame coding exon and uses an alternate in-frame splice site compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK043730.1, AK157397.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGTAC CCCTGCAGAA GTCTGCGCCG GGTTCAGCTC TCAGTCCTGC CCGCGTGCTG 
GGTGGCATTC AGCGGCCGCG CCACCTCTCG GTCTTTGAGT TTGAGTCTGA TGGCTTCCTG 
GGGTCTCCGG AGCCTACAGC TTCCTCCTCT CCGGTTACCA CTCTTACACA GACCATGCAC 
AACCTCGCTG GGCTCGGCAG TGAGCCTCCA AAAGCTCAAG TAGGAAGCCT GTCGTTCCAG 
AACAGGCTGG CAGACCTATC CCTGTCCAGG CGCACCTCTG AGTGCTCCCT GTCATCTGAG 
TCCTCAGAAT CTTCGGATGC AGGTCTGTGC ATGGACTCCC CCAGCCCTGT GGACCCGCAG 
ATGGCAGAGC GCACGTTTGA ACAGGCCATT CAGGCAGCCA GTCGGGTCAT TCAAAATGAG 
CAGTTTACCA TAAAACGCTT CCGATCCTTA CCAGTGAGGC TGCTGGAACA CAGTCCGGTG 
CTGCAGAGCA TCACCAACTC CCGAGCACTG GACAGCTGGA GGAAAACTGA AGCAGGCTAC 
CGAGCCGCCG CCAATAGTCC TGGGGAGGAC AAAGAGAATG TGCGCTTCCA GAGACCAAGA 
TGGAGGAGCT CCAGGGAGAG AAGGTGTCTT ACCACTGAGT GGAAGATGGA AGTAGAGGAG 
CTGAGCCCGG TGGCACAGTC TTCTTCCTTG ACTCCTGTCG AAAGGGCTTC TGAAGAAGAT 
GACGGATTTG TGGACATCCT GGAGAGTGAT TTAAAGGATG ACGAGAAGGT CCCCGCGGGC 
ATGGAGAACC TCATTAGTGC CCCACTGGTC AAAAAGCTGG ATAAGGAAGA GGAACAGGAT 
CTCATCATGT TCAGCAAGTG CCAGAGGCTC TTCCGCTCCC CATCCATGCC ATGCAGTGTG 
ATCCGACCCA TCCTCAAGAG GCTAGAGCGG CCCCAGGACC GGGATGTGCC TGTCCAGAGC 
AAGCGCAGGA AAAGTGTGAC ACCCCTGGAA GAGCAGCAGC TTGAAGAACC TAAGGCCCGT 
GTCTTTCGCT CAAAGTCGCT GTGTCATGAG ATTGAGAACA TCCTGGATAG TGACCACCGT 
GGACTGATCG GAGATTACTC TAAGGCCTTC CTCCTGCAGA CCGTGGATGG CAAACACCAA 
GACCTTAAGT ACATCTCACC AGAAACTATG GTGGCCCTGT TAACAGGCAA GTTCAGCAAC 
ATCGTGGAGA AATTTGTCAT TGTGGACTGC AGATACCCCT ATGAGTATGA AGGCGGGCAT 
ATCAAGAATG CTGTGAACCT GCCCCTGGAA CGGGATGCTG AGACCTTTCT GCTGCAGCGT 
CCCATCATGC CTTGTAGCCT GGACAAGAGA ATCATCCTCA TTTTCCACTG TGAATTCTCG 
TCTGAGCGTG GACCACGAAT GTGCCGCTTC ATCAGGGAAC GGGACCGTGC AGCTAACGAC 
TACCCCAGCC TGTACTACCC GGAGATGTAC ATCCTCAAAG GCGGCTACAA GGAGTTCTTC 
CCACAGCATC CGAACTTTTG TGAGCCCCAG GACTACCGAC CCATGAACCA CGAGGCTTTC 
AGGGATGAGC TGAGGAACTT TCGCCTTAAG ACTCGCAGCT GGGCTGGGGA ACGGAGCAGG 
AGGGAACTTT GTAGCAGGCT GCAAGACCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001104545.1
CDS	234..1886
Translation	MEVPLQKSAPGSALSPARVLGGIQRPRHLSVFEFESDGFLGSPEPTASSSPVTTLTQTMHNLAGLGSEPPKAQVGSLSFQNRLADLSLSRRTSECSLSSESSESSDAGLCMDSPSPVDPQMAERTFEQAIQAASRVIQNEQFTIKRFRSLPVRLLEHSPVLQSITNSRALDSWRKTEAGYRAAANSPGEDKENVRFQRPRWRSSRERRCLTTEWKMEVEELSPVAQSSSLTPVERASEEDDGFVDILESDLKDDEKVPAGMENLISAPLVKKLDKEEEQDLIMFSKCQRLFRSPSMPCSVIRPILKRLERPQDRDVPVQSKRRKSVTPLEEQQLEEPKARVFRSKSLCHEIENILDSDHRGLIGDYSKAFLLQTVDGKHQDLKYISPETMVALLTGKFSNIVEKFVIVDCRYPYEYEGGHIKNAVNLPLERDAETFLLQRPIMPCSLDKRIILIFHCEFSSERGPRMCRFIRERDRAANDYPSLYYPEMYILKGGYKEFFPQHPNFCEPQDYRPMNHEAFRDELRNFRLKTRSWAGERSRRELCSRLQDQ

Target ORF information:

RefSeq Version	NM_001111075.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus cell division cycle 25B (Cdc25b), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001111075.4

ORF Insert Sequence:

ATGGAGGTAC CCCTGCAGAA GTCTGCGCCG GGTTCAGCTC TCAGTCCTGC CCGCGTGCTG 
GGTGGCATTC AGCGGCCGCG CCACCTCTCG GTCTTTGAGT TTGAGTCTGA TGGCTTCCTG 
GGGTCTCCGG AGCCTACAGC TTCCTCCTCT CCGGTTACCA CTCTTACACA GACCATGCAC 
AACCTCGCTG GGCTCGGCAG TGAGCCTCCA AAAGCTCAAG TAGGAAGCCT GTCGTTCCAG 
AACAGGCTGG CAGACCTATC CCTGTCCAGG CGCACCTCTG AGTGCTCCCT GTCATCTGAG 
TCCTCAGAAT CTTCGGATGC AGGTCTGTGC ATGGACTCCC CCAGCCCTGT GGACCCGCAG 
ATGGCAGAGC GCACGTTTGA ACAGGCCATT CAGGCAGCCA GTCGGGTCAT TCAAAATGAG 
CAGTTTACCA TAAAACGCTT CCGATCCTTA CCAGTGAGGC TGCTGGAACA CAGTCCGGTG 
CTGCAGAGCA TCACCAACTC CCGAGCACTG GACAGCTGGA GGAAAACTGA AGCAGGCTAC 
CGAGCCGCCG CCAATAGTCC TGGGGAGGAC AAAGAGAATG TGCGCTTCCA GAGACCAAGA 
TGGAGGAGCT CCAGGGAGAG AAGGTGTCTT ACCACTGAGT GGAAGATGGA AGTAGAGGAG 
CTGAGCCCGG TGGCACAGTC TTCTTCCTTG ACTCCTGTCG AAAGGGCTTC TGAAGAAGAT 
GACGGATTTG TGGACATCCT GGAGAGTGAT TTAAAGGATG ACGAGAAGGT CCCCGCGGGC 
ATGGAGAACC TCATTAGTGC CCCACTGGTC AAAAAGCTGG ATAAGGAAGA GGAACAGGAT 
CTCATCATGT TCAGCAAGTG CCAGAGGCTC TTCCGCTCCC CATCCATGCC ATGCAGTGTG 
ATCCGACCCA TCCTCAAGAG GCTAGAGCGG CCCCAGGACC GGGATGTGCC TGTCCAGAGC 
AAGCGCAGGA AAAGTGTGAC ACCCCTGGAA GAGCAGCAGC TTGAAGAACC TAAGGCCCGT 
GTCTTTCGCT CAAAGTCGCT GTGTCATGAG ATTGAGAACA TCCTGGATAG TGACCACCGT 
GGACTGATCG GAGATTACTC TAAGGCCTTC CTCCTGCAGA CCGTGGATGG CAAACACCAA 
GACCTTAAGT ACATCTCACC AGAAACTATG GTGGCCCTGT TAACAGGCAA GTTCAGCAAC 
ATCGTGGAGA AATTTGTCAT TGTGGACTGC AGATACCCCT ATGAGTATGA AGGCGGGCAT 
ATCAAGAATG CTGTGAACCT GCCCCTGGAA CGGGATGCTG AGACCTTTCT GCTGCAGCGT 
CCCATCATGC CTTGTAGCCT GGACAAGAGA ATCATCCTCA TTTTCCACTG TGAATTCTCG 
TCTGAGCGTG GACCACGAAT GTGCCGCTTC ATCAGGGAAC GGGACCGTGC AGCTAACGAC 
TACCCCAGCC TGTACTACCC GGAGATGTAC ATCCTCAAAG GCGGCTACAA GGAGTTCTTC 
CCACAGCATC CGAACTTTTG TGAGCCCCAG GACTACCGAC CCATGAACCA CGAGGCTTTC 
AGGGATGAGC TGAGGAACTT TCGCCTTAAG ACTCGCAGCT GGGCTGGGGA ACGGAGCAGG 
AGGGAACTTT GTAGCAGGCT GCAAGACCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc25b ( NM_023117.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_075606.1 Mus musculus cell division cycle 25B (Cdc25b), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu20140
Clone ID Related Accession (Same CDS sequence)	NM_023117.4
Accession Version	NM_023117.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1731bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	M-phase inducer phosphatase 2 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL831736.16, BQ561430.1, W54021.1, BC057568.1 and AL808128.4. On Mar 21, 2012 this sequence version replaced NM_023117.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK153903.1, SRR1660811.228555.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGTAC CCCTGCAGAA GTCTGCGCCG GGTTCAGCTC TCAGTCCTGC CCGCGTGCTG 
GGTGGCATTC AGCGGCCGCG CCACCTCTCG GTCTTTGAGT TTGAGTCTGA TGGCTTCCTG 
GGGTCTCCGG AGCCTACAGC TTCCTCCTCT CCGGTTACCA CTCTTACACA GACCATGCAC 
AACCTCGCTG GGCTCGGCAG TGAGCCTCCA AAAGCTCAAG TAGGAAGCCT GTCGTTCCAG 
AACAGGCTGG CAGACCTATC CCTGTCCAGG CGCACCTCTG AGTGCTCCCT GTCATCTGAG 
TCCTCAGAAT CTTCGGATGC AGGTCTGTGC ATGGACTCCC CCAGCCCTGT GGACCCGCAG 
ATGGCAGAGC GCACGTTTGA ACAGGCCATT CAGGCAGCCA GTCGGGTCAT TCAAAATGAG 
CAGTTTACCA TAAAACGCTT CCGATCCTTA CCAGTGAGGC TGCTGGAACA CAGTCCGGTG 
CTGCAGAGCA TCACCAACTC CCGAGCACTG GACAGCTGGA GGAAAACTGA AGCAGGCTAC 
CGAGCCGCCG CCAATAGTCC TGGGGAGGAC AAAGAGAATG ATGGATATAT CTTCAAGATG 
CCACAGGAGC TCCCTCATTC CAGCTCTGCC CAAGCTTTGG CAGAATGGGT CAGCCGCAGA 
CAGGCCTTTA CCCAGAGGCC CAGCTCAGCC CCTGACTTGA TGTGTCTTAC CACTGAGTGG 
AAGATGGAAG TAGAGGAGCT GAGCCCGGTG GCACAGTCTT CTTCCTTGAC TCCTGTCGAA 
AGGGCTTCTG AAGAAGATGA CGGATTTGTG GACATCCTGG AGAGTGATTT AAAGGATGAC 
GAGAAGGTCC CCGCGGGCAT GGAGAACCTC ATTAGTGCCC CACTGGTCAA AAAGCTGGAT 
AAGGAAGAGG AACAGGATCT CATCATGTTC AGCAAGTGCC AGAGGCTCTT CCGCTCCCCA 
TCCATGCCAT GCAGTGTGAT CCGACCCATC CTCAAGAGGC TAGAGCGGCC CCAGGACCGG 
GATGTGCCTG TCCAGAGCAA GCGCAGGAAA AGTGTGACAC CCCTGGAAGA GCAGCAGCTT 
GAAGAACCTA AGGCCCGTGT CTTTCGCTCA AAGTCGCTGT GTCATGAGAT TGAGAACATC 
CTGGATAGTG ACCACCGTGG ACTGATCGGA GATTACTCTA AGGCCTTCCT CCTGCAGACC 
GTGGATGGCA AACACCAAGA CCTTAAGTAC ATCTCACCAG AAACTATGGT GGCCCTGTTA 
ACAGGCAAGT TCAGCAACAT CGTGGAGAAA TTTGTCATTG TGGACTGCAG ATACCCCTAT 
GAGTATGAAG GCGGGCATAT CAAGAATGCT GTGAACCTGC CCCTGGAACG GGATGCTGAG 
ACCTTTCTGC TGCAGCGTCC CATCATGCCT TGTAGCCTGG ACAAGAGAAT CATCCTCATT 
TTCCACTGTG AATTCTCGTC TGAGCGTGGA CCACGAATGT GCCGCTTCAT CAGGGAACGG 
GACCGTGCAG CTAACGACTA CCCCAGCCTG TACTACCCGG AGATGTACAT CCTCAAAGGC 
GGCTACAAGG AGTTCTTCCC ACAGCATCCG AACTTTTGTG AGCCCCAGGA CTACCGACCC 
ATGAACCACG AGGCTTTCAG GGATGAGCTG AGGAACTTTC GCCTTAAGAC TCGCAGCTGG 
GCTGGGGAAC GGAGCAGGAG GGAACTTTGT AGCAGGCTGC AAGACCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_075606.1
CDS	234..1964
Translation	MEVPLQKSAPGSALSPARVLGGIQRPRHLSVFEFESDGFLGSPEPTASSSPVTTLTQTMHNLAGLGSEPPKAQVGSLSFQNRLADLSLSRRTSECSLSSESSESSDAGLCMDSPSPVDPQMAERTFEQAIQAASRVIQNEQFTIKRFRSLPVRLLEHSPVLQSITNSRALDSWRKTEAGYRAAANSPGEDKENDGYIFKMPQELPHSSSAQALAEWVSRRQAFTQRPSSAPDLMCLTTEWKMEVEELSPVAQSSSLTPVERASEEDDGFVDILESDLKDDEKVPAGMENLISAPLVKKLDKEEEQDLIMFSKCQRLFRSPSMPCSVIRPILKRLERPQDRDVPVQSKRRKSVTPLEEQQLEEPKARVFRSKSLCHEIENILDSDHRGLIGDYSKAFLLQTVDGKHQDLKYISPETMVALLTGKFSNIVEKFVIVDCRYPYEYEGGHIKNAVNLPLERDAETFLLQRPIMPCSLDKRIILIFHCEFSSERGPRMCRFIRERDRAANDYPSLYYPEMYILKGGYKEFFPQHPNFCEPQDYRPMNHEAFRDELRNFRLKTRSWAGERSRRELCSRLQDQ

Target ORF information:

RefSeq Version	NM_023117.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus cell division cycle 25B (Cdc25b), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023117.4

ORF Insert Sequence:

ATGGAGGTAC CCCTGCAGAA GTCTGCGCCG GGTTCAGCTC TCAGTCCTGC CCGCGTGCTG 
GGTGGCATTC AGCGGCCGCG CCACCTCTCG GTCTTTGAGT TTGAGTCTGA TGGCTTCCTG 
GGGTCTCCGG AGCCTACAGC TTCCTCCTCT CCGGTTACCA CTCTTACACA GACCATGCAC 
AACCTCGCTG GGCTCGGCAG TGAGCCTCCA AAAGCTCAAG TAGGAAGCCT GTCGTTCCAG 
AACAGGCTGG CAGACCTATC CCTGTCCAGG CGCACCTCTG AGTGCTCCCT GTCATCTGAG 
TCCTCAGAAT CTTCGGATGC AGGTCTGTGC ATGGACTCCC CCAGCCCTGT GGACCCGCAG 
ATGGCAGAGC GCACGTTTGA ACAGGCCATT CAGGCAGCCA GTCGGGTCAT TCAAAATGAG 
CAGTTTACCA TAAAACGCTT CCGATCCTTA CCAGTGAGGC TGCTGGAACA CAGTCCGGTG 
CTGCAGAGCA TCACCAACTC CCGAGCACTG GACAGCTGGA GGAAAACTGA AGCAGGCTAC 
CGAGCCGCCG CCAATAGTCC TGGGGAGGAC AAAGAGAATG ATGGATATAT CTTCAAGATG 
CCACAGGAGC TCCCTCATTC CAGCTCTGCC CAAGCTTTGG CAGAATGGGT CAGCCGCAGA 
CAGGCCTTTA CCCAGAGGCC CAGCTCAGCC CCTGACTTGA TGTGTCTTAC CACTGAGTGG 
AAGATGGAAG TAGAGGAGCT GAGCCCGGTG GCACAGTCTT CTTCCTTGAC TCCTGTCGAA 
AGGGCTTCTG AAGAAGATGA CGGATTTGTG GACATCCTGG AGAGTGATTT AAAGGATGAC 
GAGAAGGTCC CCGCGGGCAT GGAGAACCTC ATTAGTGCCC CACTGGTCAA AAAGCTGGAT 
AAGGAAGAGG AACAGGATCT CATCATGTTC AGCAAGTGCC AGAGGCTCTT CCGCTCCCCA 
TCCATGCCAT GCAGTGTGAT CCGACCCATC CTCAAGAGGC TAGAGCGGCC CCAGGACCGG 
GATGTGCCTG TCCAGAGCAA GCGCAGGAAA AGTGTGACAC CCCTGGAAGA GCAGCAGCTT 
GAAGAACCTA AGGCCCGTGT CTTTCGCTCA AAGTCGCTGT GTCATGAGAT TGAGAACATC 
CTGGATAGTG ACCACCGTGG ACTGATCGGA GATTACTCTA AGGCCTTCCT CCTGCAGACC 
GTGGATGGCA AACACCAAGA CCTTAAGTAC ATCTCACCAG AAACTATGGT GGCCCTGTTA 
ACAGGCAAGT TCAGCAACAT CGTGGAGAAA TTTGTCATTG TGGACTGCAG ATACCCCTAT 
GAGTATGAAG GCGGGCATAT CAAGAATGCT GTGAACCTGC CCCTGGAACG GGATGCTGAG 
ACCTTTCTGC TGCAGCGTCC CATCATGCCT TGTAGCCTGG ACAAGAGAAT CATCCTCATT 
TTCCACTGTG AATTCTCGTC TGAGCGTGGA CCACGAATGT GCCGCTTCAT CAGGGAACGG 
GACCGTGCAG CTAACGACTA CCCCAGCCTG TACTACCCGG AGATGTACAT CCTCAAAGGC 
GGCTACAAGG AGTTCTTCCC ACAGCATCCG AACTTTTGTG AGCCCCAGGA CTACCGACCC 
ATGAACCACG AGGCTTTCAG GGATGAGCTG AGGAACTTTC GCCTTAAGAC TCGCAGCTGG 
GCTGGGGAAC GGAGCAGGAG GGAACTTTGT AGCAGGCTGC AAGACCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

APC/C
Current biology : CB27(10)1462-1476.e5(2017 May)
Rattani A,Ballesteros Mejia R,Roberts K,Roig MB,Godwin J,Hopkins M,Eguren M,Sanchez-Pulido L,Okaz E,Ogushi S,Wolna M,Metson J,Pend?s AM,Malumbres M,Nov?k B,Herbert M,Nasmyth K

CDC25B is involved in the centrosomal microtubule nucleation in two-cell stage mouse embryos.
Development, growth & differentiation58(9)714-726(2016 Dec)
Cui C,Zang T,Cao Y,Qin X,Zhang X