Car1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Car1
Entrez Gene ID	12346
Full Name	carbonic anhydrase 1
Synonyms	AW555628,Ca1,Car-1
General protein information	Preferred Names carbonic anhydrase 1 Names carbonic anhydrase 1 CA-I carbonate dehydratase I carbonic anhydrase I
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3 A1\|3 3.18 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_011248137.1	XP_011246439.1	carbonic anhydrase 1 isoform X1
NM_001083957.1	NP_001077426.1	carbonic anhydrase 1
NM_009799.4	NP_033929.2	carbonic anhydrase 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	CA1	NP_001122301.1
	CA1	XP_002805445.1
Bos taurus (cattle)	CA1	NP_001068934.1
Mus musculus (house mouse)	Car1	NP_033929.2
Pan troglodytes (chimpanzee)	CA1	NP_001182062.1
Bos taurus (cattle)	LOC784254	XP_002692874.1
Rattus norvegicus (Norway rat)	Ca1	NP_001101130.1
Xenopus tropicalis (tropical clawed frog)	LOC100496485	XP_002939198.1
Canis lupus familiaris (dog)	CA1	NP_001138643.1

Gene Ontology
Bibliography

Related articles in PubMed

Generation of an inducible colon-specific Cre enzyme mouse line for colon cancer research.
Tetteh PW, Kretzschmar K, Begthel H, van den Born M, Korving J, Morsink F, Farin H, van Es JH, Offerhaus GJ, Clevers H
Proceedings of the National Academy of Sciences of the United States of America113(42)11859-11864(2016 10)

Carbonic anhydrase enzymes regulate mast cell-mediated inflammation.
Henry EK, Sy CB, Inclan-Rico JM, Espinosa V, Ghanny SS, Dwyer DF, Soteropoulos P, Rivera A, Siracusa MC
The Journal of experimental medicine213(9)1663-73(2016 Aug)

Purification and Transcriptomic Analysis of Mouse Fetal Leydig Cells Reveals Candidate Genes for Specification of Gonadal Steroidogenic Cells.
McClelland KS, Bell K, Larney C, Harley VR, Sinclair AH, Oshlack A, Koopman P, Bowles J
Biology of reproduction92(6)145(2015 Jun)

GATA4 and GATA6 regulate intestinal epithelial cytodifferentiation during development.
Walker EM, Thompson CA, Battle MA
Developmental biology392(2)283-94(2014 Aug)

Transgenic mice over-expressing carbonic anhydrase I showed aggravated joint inflammation and tissue destruction.
Zheng Y, Wang L, Zhang W, Xu H, Chang X
BMC musculoskeletal disorders13256(2012 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

During inner ear development, transcripts of four cytosolic isozymes (Car1, Car2, Car3, and Car13), two membrane-bound isozymes (Car12 and Car14), and two CA-related proteins (Car8 and Car11) were expressed at higher levels than other isozymes.
Title: A systematic survey of carbonic anhydrase mRNA expression during mammalian inner ear development.

Over-expression of CA1 may aggravate joint inflammation and tissue destruction in the transgenic mice.
Title: Transgenic mice over-expressing carbonic anhydrase I showed aggravated joint inflammation and tissue destruction.

Taken together, these data show that Ldb1 forms a regulatory complex to maintain Car1 expression in erythroid cells.
Title: Ldb1 regulates carbonic anhydrase 1 during erythroid differentiation.

Regulatory dendritic cells pulsed with carbonic anhydrase I suppress development of colitis in a severe combined immunodeficiency (SCID) mouse transfer model by inducing the differentiation of regulatory T cells in an antigen-specific manner.
Title: Regulatory dendritic cells pulsed with carbonic anhydrase I protect mice from colitis induced by CD4+CD25- T cells.

The following Car1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Car1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu02886	NM_009799.4 Latest version!	Mus musculus carbonic anhydrase 1 (Car1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu02886	NM_001083957.1 Latest version!	Mus musculus carbonic anhydrase 1 (Car1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu02886	XM_011248137.1 Latest version!	Mus musculus carbonic anhydrase 1 (Car1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Car1 ( NM_001083957.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001077426.1 Mus musculus carbonic anhydrase 1 (Car1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu02886
Clone ID Related Accession (Same CDS sequence)	XM_011248137.1 , NM_001083957.1 , NM_009799.4
Accession Version	NM_001083957.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	carbonic anhydrase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK162331.1 and AV374892.1. Transcript Variant: This variant (2) has a different 5' UTR compared to transcript variant 1. Both transcript variants (1 and 2) encode the same protein. This variant represents use of an alternate downstream promoter and results in colon epithelia cell expression. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK162331.1, AK033719.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849389, SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAAGTG CAGACTGGGG ATATGGAAGC GAAAATGGTC CTGACCAATG GAGCAAGCTG 
TATCCCATTG CCAATGGAAA CAACCAGTCT CCTATTGATA TTAAAACCAG TGAAGCCAAT 
CATGACTCCT CTCTGAAACC ACTCAGCATC TCCTATAATC CTGCAACTGC CAAAGAAATT 
GTTAACGTGG GACATTCTTT CCATGTAATT TTTGATGACA GTAGCAACCA ATCTGTTCTG 
AAAGGTGGCC CTCTTGCTGA TAGCTATCGG CTCACTCAGT TCCATTTTCA CTGGGGCAAC 
TCAAACGACC ATGGCTCTGA GCACACCGTG GATGGAACTA GATATTCTGG AGAGCTTCAC 
TTAGTTCACT GGAATTCTGC AAAGTACTCC AGTGCTTCTG AAGCCATCTC CAAGGCTGAT 
GGCCTGGCAA TCCTTGGCGT TTTGATGAAG GTTGGTCCAG CCAACCCAAG CCTGCAGAAA 
GTACTTGATG CTCTAAACTC AGTTAAAACT AAGGGAAAAC GAGCCCCATT CACAAATTTT 
GACCCATCCA GTCTGCTTCC TTCATCTCTG GATTACTGGA CCTACTTTGG CTCTCTGACT 
CACCCTCCTC TTCATGAAAG TGTGACCTGG GTGATCTGCA AGGATAGCAT CAGTCTAAGC 
CCAGAGCAGC TGGCCCAGCT CCGTGGTCTT CTGTCAAGTG CAGAGGGAGA GCCTGCAGTT 
CCAGTTCTGA GCAACCACCG TCCACCCCAA CCCCTGAAGG GCAGAACAGT CAGAGCCTCA 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001077426.1
CDS	77..862
Translation	MASADWGYGSENGPDQWSKLYPIANGNNQSPIDIKTSEANHDSSLKPLSISYNPATAKEIVNVGHSFHVIFDDSSNQSVLKGGPLADSYRLTQFHFHWGNSNDHGSEHTVDGTRYSGELHLVHWNSAKYSSASEAISKADGLAILGVLMKVGPANPSLQKVLDALNSVKTKGKRAPFTNFDPSSLLPSSLDYWTYFGSLTHPPLHESVTWVICKDSISLSPEQLAQLRGLLSSAEGEPAVPVLSNHRPPQPLKGRTVRASF

Target ORF information:

RefSeq Version	NM_001083957.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus carbonic anhydrase 1 (Car1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083957.1

ORF Insert Sequence:

ATGGCAAGTG CAGACTGGGG ATATGGAAGC GAAAATGGTC CTGACCAATG GAGCAAGCTG 
TATCCCATTG CCAATGGAAA CAACCAGTCT CCTATTGATA TTAAAACCAG TGAAGCCAAT 
CATGACTCCT CTCTGAAACC ACTCAGCATC TCCTATAATC CTGCAACTGC CAAAGAAATT 
GTTAACGTGG GACATTCTTT CCATGTAATT TTTGATGACA GTAGCAACCA ATCTGTTCTG 
AAAGGTGGCC CTCTTGCTGA TAGCTATCGG CTCACTCAGT TCCATTTTCA CTGGGGCAAC 
TCAAACGACC ATGGCTCTGA GCACACCGTG GATGGAACTA GATATTCTGG AGAGCTTCAC 
TTAGTTCACT GGAATTCTGC AAAGTACTCC AGTGCTTCTG AAGCCATCTC CAAGGCTGAT 
GGCCTGGCAA TCCTTGGCGT TTTGATGAAG GTTGGTCCAG CCAACCCAAG CCTGCAGAAA 
GTACTTGATG CTCTAAACTC AGTTAAAACT AAGGGAAAAC GAGCCCCATT CACAAATTTT 
GACCCATCCA GTCTGCTTCC TTCATCTCTG GATTACTGGA CCTACTTTGG CTCTCTGACT 
CACCCTCCTC TTCATGAAAG TGTGACCTGG GTGATCTGCA AGGATAGCAT CAGTCTAAGC 
CCAGAGCAGC TGGCCCAGCT CCGTGGTCTT CTGTCAAGTG CAGAGGGAGA GCCTGCAGTT 
CCAGTTCTGA GCAACCACCG TCCACCCCAA CCCCTGAAGG GCAGAACAGT CAGAGCCTCA 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Car1 ( NM_009799.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033929.2 Mus musculus carbonic anhydrase 1 (Car1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu02886
Clone ID Related Accession (Same CDS sequence)	XM_011248137.1 , NM_001083957.1 , NM_009799.4
Accession Version	NM_009799.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	carbonic anhydrase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB842334.1, AK003066.1, AK162331.1 and AV374892.1. On Apr 17, 2007 this sequence version replaced NM_009799.3. Transcript Variant: This variant (1) represents the longer transcript. Both transcript variants (1 and 2) encode the same protein. This variant represents use of an upstream long terminal repeat (LTR) promoter and exon sequence, and results in erythroid expression. (PMID: 2571923). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK167969.1, AK146372.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135, SAMN01164140 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAAGTG CAGACTGGGG ATATGGAAGC GAAAATGGTC CTGACCAATG GAGCAAGCTG 
TATCCCATTG CCAATGGAAA CAACCAGTCT CCTATTGATA TTAAAACCAG TGAAGCCAAT 
CATGACTCCT CTCTGAAACC ACTCAGCATC TCCTATAATC CTGCAACTGC CAAAGAAATT 
GTTAACGTGG GACATTCTTT CCATGTAATT TTTGATGACA GTAGCAACCA ATCTGTTCTG 
AAAGGTGGCC CTCTTGCTGA TAGCTATCGG CTCACTCAGT TCCATTTTCA CTGGGGCAAC 
TCAAACGACC ATGGCTCTGA GCACACCGTG GATGGAACTA GATATTCTGG AGAGCTTCAC 
TTAGTTCACT GGAATTCTGC AAAGTACTCC AGTGCTTCTG AAGCCATCTC CAAGGCTGAT 
GGCCTGGCAA TCCTTGGCGT TTTGATGAAG GTTGGTCCAG CCAACCCAAG CCTGCAGAAA 
GTACTTGATG CTCTAAACTC AGTTAAAACT AAGGGAAAAC GAGCCCCATT CACAAATTTT 
GACCCATCCA GTCTGCTTCC TTCATCTCTG GATTACTGGA CCTACTTTGG CTCTCTGACT 
CACCCTCCTC TTCATGAAAG TGTGACCTGG GTGATCTGCA AGGATAGCAT CAGTCTAAGC 
CCAGAGCAGC TGGCCCAGCT CCGTGGTCTT CTGTCAAGTG CAGAGGGAGA GCCTGCAGTT 
CCAGTTCTGA GCAACCACCG TCCACCCCAA CCCCTGAAGG GCAGAACAGT CAGAGCCTCA 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033929.2
CDS	89..874
Translation	MASADWGYGSENGPDQWSKLYPIANGNNQSPIDIKTSEANHDSSLKPLSISYNPATAKEIVNVGHSFHVIFDDSSNQSVLKGGPLADSYRLTQFHFHWGNSNDHGSEHTVDGTRYSGELHLVHWNSAKYSSASEAISKADGLAILGVLMKVGPANPSLQKVLDALNSVKTKGKRAPFTNFDPSSLLPSSLDYWTYFGSLTHPPLHESVTWVICKDSISLSPEQLAQLRGLLSSAEGEPAVPVLSNHRPPQPLKGRTVRASF

Target ORF information:

RefSeq Version	NM_009799.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus carbonic anhydrase 1 (Car1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009799.4

ORF Insert Sequence:

ATGGCAAGTG CAGACTGGGG ATATGGAAGC GAAAATGGTC CTGACCAATG GAGCAAGCTG 
TATCCCATTG CCAATGGAAA CAACCAGTCT CCTATTGATA TTAAAACCAG TGAAGCCAAT 
CATGACTCCT CTCTGAAACC ACTCAGCATC TCCTATAATC CTGCAACTGC CAAAGAAATT 
GTTAACGTGG GACATTCTTT CCATGTAATT TTTGATGACA GTAGCAACCA ATCTGTTCTG 
AAAGGTGGCC CTCTTGCTGA TAGCTATCGG CTCACTCAGT TCCATTTTCA CTGGGGCAAC 
TCAAACGACC ATGGCTCTGA GCACACCGTG GATGGAACTA GATATTCTGG AGAGCTTCAC 
TTAGTTCACT GGAATTCTGC AAAGTACTCC AGTGCTTCTG AAGCCATCTC CAAGGCTGAT 
GGCCTGGCAA TCCTTGGCGT TTTGATGAAG GTTGGTCCAG CCAACCCAAG CCTGCAGAAA 
GTACTTGATG CTCTAAACTC AGTTAAAACT AAGGGAAAAC GAGCCCCATT CACAAATTTT 
GACCCATCCA GTCTGCTTCC TTCATCTCTG GATTACTGGA CCTACTTTGG CTCTCTGACT 
CACCCTCCTC TTCATGAAAG TGTGACCTGG GTGATCTGCA AGGATAGCAT CAGTCTAAGC 
CCAGAGCAGC TGGCCCAGCT CCGTGGTCTT CTGTCAAGTG CAGAGGGAGA GCCTGCAGTT 
CCAGTTCTGA GCAACCACCG TCCACCCCAA CCCCTGAAGG GCAGAACAGT CAGAGCCTCA 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Car1 ( XM_011248137.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246439.1 Mus musculus carbonic anhydrase 1 (Car1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu02886
Clone ID Related Accession (Same CDS sequence)	XM_011248137.1 , NM_001083957.1 , NM_009799.4
Accession Version	XM_011248137.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	carbonic anhydrase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAGTG CAGACTGGGG ATATGGAAGC GAAAATGGTC CTGACCAATG GAGCAAGCTG 
TATCCCATTG CCAATGGAAA CAACCAGTCT CCTATTGATA TTAAAACCAG TGAAGCCAAT 
CATGACTCCT CTCTGAAACC ACTCAGCATC TCCTATAATC CTGCAACTGC CAAAGAAATT 
GTTAACGTGG GACATTCTTT CCATGTAATT TTTGATGACA GTAGCAACCA ATCTGTTCTG 
AAAGGTGGCC CTCTTGCTGA TAGCTATCGG CTCACTCAGT TCCATTTTCA CTGGGGCAAC 
TCAAACGACC ATGGCTCTGA GCACACCGTG GATGGAACTA GATATTCTGG AGAGCTTCAC 
TTAGTTCACT GGAATTCTGC AAAGTACTCC AGTGCTTCTG AAGCCATCTC CAAGGCTGAT 
GGCCTGGCAA TCCTTGGCGT TTTGATGAAG GTTGGTCCAG CCAACCCAAG CCTGCAGAAA 
GTACTTGATG CTCTAAACTC AGTTAAAACT AAGGGAAAAC GAGCCCCATT CACAAATTTT 
GACCCATCCA GTCTGCTTCC TTCATCTCTG GATTACTGGA CCTACTTTGG CTCTCTGACT 
CACCCTCCTC TTCATGAAAG TGTGACCTGG GTGATCTGCA AGGATAGCAT CAGTCTAAGC 
CCAGAGCAGC TGGCCCAGCT CCGTGGTCTT CTGTCAAGTG CAGAGGGAGA GCCTGCAGTT 
CCAGTTCTGA GCAACCACCG TCCACCCCAA CCCCTGAAGG GCAGAACAGT CAGAGCCTCA 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246439.1
CDS	238..1023
Translation	MASADWGYGSENGPDQWSKLYPIANGNNQSPIDIKTSEANHDSSLKPLSISYNPATAKEIVNVGHSFHVIFDDSSNQSVLKGGPLADSYRLTQFHFHWGNSNDHGSEHTVDGTRYSGELHLVHWNSAKYSSASEAISKADGLAILGVLMKVGPANPSLQKVLDALNSVKTKGKRAPFTNFDPSSLLPSSLDYWTYFGSLTHPPLHESVTWVICKDSISLSPEQLAQLRGLLSSAEGEPAVPVLSNHRPPQPLKGRTVRASF

Target ORF information:

RefSeq Version	XM_011248137.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus carbonic anhydrase 1 (Car1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248137.1

ORF Insert Sequence:

ATGGCAAGTG CAGACTGGGG ATATGGAAGC GAAAATGGTC CTGACCAATG GAGCAAGCTG 
TATCCCATTG CCAATGGAAA CAACCAGTCT CCTATTGATA TTAAAACCAG TGAAGCCAAT 
CATGACTCCT CTCTGAAACC ACTCAGCATC TCCTATAATC CTGCAACTGC CAAAGAAATT 
GTTAACGTGG GACATTCTTT CCATGTAATT TTTGATGACA GTAGCAACCA ATCTGTTCTG 
AAAGGTGGCC CTCTTGCTGA TAGCTATCGG CTCACTCAGT TCCATTTTCA CTGGGGCAAC 
TCAAACGACC ATGGCTCTGA GCACACCGTG GATGGAACTA GATATTCTGG AGAGCTTCAC 
TTAGTTCACT GGAATTCTGC AAAGTACTCC AGTGCTTCTG AAGCCATCTC CAAGGCTGAT 
GGCCTGGCAA TCCTTGGCGT TTTGATGAAG GTTGGTCCAG CCAACCCAAG CCTGCAGAAA 
GTACTTGATG CTCTAAACTC AGTTAAAACT AAGGGAAAAC GAGCCCCATT CACAAATTTT 
GACCCATCCA GTCTGCTTCC TTCATCTCTG GATTACTGGA CCTACTTTGG CTCTCTGACT 
CACCCTCCTC TTCATGAAAG TGTGACCTGG GTGATCTGCA AGGATAGCAT CAGTCTAAGC 
CCAGAGCAGC TGGCCCAGCT CCGTGGTCTT CTGTCAAGTG CAGAGGGAGA GCCTGCAGTT 
CCAGTTCTGA GCAACCACCG TCCACCCCAA CCCCTGAAGG GCAGAACAGT CAGAGCCTCA 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Generation of an inducible colon-specific Cre enzyme mouse line for colon cancer research.
Proceedings of the National Academy of Sciences of the United States of America113(42)11859-11864(2016 10)
Tetteh PW,Kretzschmar K,Begthel H,van den Born M,Korving J,Morsink F,Farin H,van Es JH,Offerhaus GJ,Clevers H

Carbonic anhydrase enzymes regulate mast cell-mediated inflammation.
The Journal of experimental medicine213(9)1663-73(2016 Aug)
Henry EK,Sy CB,Inclan-Rico JM,Espinosa V,Ghanny SS,Dwyer DF,Soteropoulos P,Rivera A,Siracusa MC

Purification and Transcriptomic Analysis of Mouse Fetal Leydig Cells Reveals Candidate Genes for Specification of Gonadal Steroidogenic Cells.
Biology of reproduction92(6)145(2015 Jun)
McClelland KS,Bell K,Larney C,Harley VR,Sinclair AH,Oshlack A,Koopman P,Bowles J

GATA4 and GATA6 regulate intestinal epithelial cytodifferentiation during development.
Developmental biology392(2)283-94(2014 Aug)
Walker EM,Thompson CA,Battle MA

Transgenic mice over-expressing carbonic anhydrase I showed aggravated joint inflammation and tissue destruction.
BMC musculoskeletal disorders13256(2012 Dec)
Zheng Y,Wang L,Zhang W,Xu H,Chang X