Atp2a2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Atp2a2
Entrez Gene ID	11938
Full Name	ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
Synonyms	9530097L16Rik,D5Wsu150e,SERCA2,SERCA2B,Serca2a,mKIAA4195
General protein information	Preferred Names ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 Names sarcoplasmic/endoplasmic reticulum calcium ATPase 2 SERCA2a isoform SR Ca(2+)-ATPase 2 calcium pump 2 calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform endoplasmic reticulum class 1/2 Ca(2+) ATPase sarco/endoplasmic reticulum Ca2+-ATPase 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5 62.38 cM\|5 F

mRNA and Protein(s)

mRNA	Protein	Name
NM_001110140.3	NP_001103610.1	sarcoplasmic/endoplasmic reticulum calcium ATPase 2 isoform b
NM_009722.3	NP_033852.1	sarcoplasmic/endoplasmic reticulum calcium ATPase 2 isoform a

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ATP2A2	NP_001178359.1
Rattus norvegicus (Norway rat)	Atp2a2	NP_001104293.1
Mus musculus (house mouse)	Atp2a2	NP_001103610.1
Pan troglodytes (chimpanzee)	ATP2A2	XP_001141455.1
Gallus gallus (chicken)	ATP2A2	NP_001258903.1
Xenopus tropicalis (tropical clawed frog)	atp2a2	XP_004910568.1
Homo sapiens (human)	ATP2A2	NP_733765.1
Canis lupus familiaris (dog)	ATP2A2	NP_001003214.1
Danio rerio (zebrafish)	atp2a2a	NP_957259.1

Gene Ontology

The following Atp2a2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Atp2a2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu18633	NM_009722.3 Latest version!	Mus musculus ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 (Atp2a2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
OMu19022	NM_001110140.3 Latest version!	Mus musculus ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 (Atp2a2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Atp2a2 ( NM_009722.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033852.1 Mus musculus ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 (Atp2a2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu18633
Clone ID Related Accession (Same CDS sequence)	NM_009722.3
Accession Version	NM_009722.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	sarcoplasmic/endoplasmic reticulum calcium ATPase 2 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ162528.1, AK220538.1 and BC054748.1. On Nov 22, 2007 this sequence version replaced NM_009722.2. Transcript Variant: This variant (2) differs in the 3' coding region and 3' UTR, compared to variant 1. The resulting isoform (a), also known as SERCA2A, has a shorter and distinct C-terminus compared to isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ223584.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGAGAACG CTCACACAAA GACCGTGGAG GAGGTGCTGG GCCACTTCGG GGTCAACGAG 
AGCACGGGGC TGAGCTTGGA GCAGGTCAAG AAGCTCAAGG AGAGATGGGG CTCCAACGAA 
TTGCCGGCTG AAGAAGGAAA AACCTTGCTG GAACTTGTGA TTGAGCAGTT TGAAGACTTA 
CTAGTTAGAA TTTTACTGCT GGCAGCATGT ATATCTTTCG TTTTGGCTTG GTTCGAGGAA 
GGGGAAGAAA CGATTACAGC CTTTGTAGAG CCGTTTGTAA TTCTGCTTAT CTTGGTAGCC 
AATGCAATCG TGGGTGTGTG GCAGGAAAGA AATGCTGAAA ATGCAATAGA AGCTCTTAAG 
GAATATGAGC CTGAAATGGG CAAAGTGTAT CGACAGGACA GAAAGAGTGT GCAACGAATT 
AAAGCTAAAG ACATAGTTCC TGGTGATATA GTGGAAATTG CTGTTGGTGA CAAAGTTCCT 
GCTGATATTA GATTGACATC CATCAAGTCT ACAACTCTAA GAGTCGACCA GTCAATTCTT 
ACAGGTGAAT CTGTCTCCGT CATCAAGCAT ACTGACCCTG TCCCTGACCC CCGAGCTGTT 
AATCAAGACA AAAAGAACAT GCTCTTTTCT GGTACAAACA TTGCTGCTGG GAAAGCTATG 
GGAGTGGTGG TGGCAACTGG AGTTAATACT GAGATCGGCA AGATCCGGGA TGAAATGGTG 
GCAACAGAAC AGGAGAGAAC ACCCCTACAG CAGAAGCTAG ACGAGTTTGG GGAGCAGCTT 
TCCAAAGTTA TCTCCCTCAT TTGCATTGCA GTCTGGATCA TCAACATTGG GCATTTCAAT 
GACCCAGTTC ATGGTGGCTC CTGGATCAGG GGTGCCATCT ACTACTTTAA GATTGCCGTG 
GCCCTGGCTG TTGCCGCAAT CCCTGAGGGT CTGCCTGCTG TCATCACCAC CTGCTTAGCT 
CTTGGAACTC GTAGGATGGC AAAGAAAAAT GCTATCGTTC GAAGTCTGCC TTCTGTGGAG 
ACCCTTGGTT GTACTTCTGT TATCTGCTCA GATAAGACAG GCACACTTAC CACAAACCAG 
ATGTCCGTGT GCAGGATGTT CATTCTGGAC AAAGTAGAAG GTGACACTTG TTCCCTTAAT 
GAGTTCAGCA TAACTGGATC CACATATGCA CCAATTGGAG AAGTGCAAAA GGATGATAAG 
CCAGTGAAGT GCCATCAGTA TGACGGGCTT GTAGAGTTAG CCACCATCTG TGCTCTGTGT 
AATGACTCTG CTTTGGATTA TAATGAGGCA AAGGGTGTGT ATGAGAAAGT TGGAGAAGCT 
ACCGAGACTG CTCTCACGTG CCTGGTGGAG AAGATGAATG TATTTGATAC TGAGCTGAAG 
GGGCTTTCTA AAATAGAGCG TGCAAACGCC TGCAACTCGG TCATAAAGCA GCTGATGAAG 
AAGGAGTTCA CTCTGGAGTT TTCACGGGAT AGAAAATCAA TGTCCGTCTA TTGTACCCCA 
AACAAGCCAA GCCGGACATC CATGAGCAAG ATGTTTGTGA AGGGGGCTCC AGAAGGTGTC 
ATCGATAGGT GCACCCACAT CCGAGTTGGA AGTACCAAGG TCCCCATGAC TCCTGGTGTC 
AAACAGAAGA TTATGTCTGT CATTCGGGAG TGGGGCAGTG GCAGCGACAC GCTACGGTGC 
CTGGCTCTGG CCACTCATGA CAACCCACTG AAGAGAGAGG AGATGCACCT GGAAGACTCT 
GCTAACTTCA TCAAATACGA GACCAACCTG ACTTTCGTCG GCTGTGTGGG CATGCTGGAT 
CCTCCCAGGA TTGAAGTAGC CTCTTCTGTG AAGCTGTGCC GGCAAGCAGG CATCCGGGTC 
ATCATGATCA CTGGAGACAA CAAAGGCACC GCTGTGGCCA TCTGTCGCCG CATTGGCATC 
TTTGGGCAGG ATGAGGATGT GACATCAAAG GCTTTTACAG GGCGAGAGTT TGATGAATTA 
AGCCCTTCAG CCCAGAGAGA TGCCTGCTTA AATGCCCGCT GTTTTGCTCG AGTTGAACCT 
TCCCACAAGT CTAAGATTGT TGAGTTCCTT CAGTCCTTTG ATGAGATCAC AGCTATGACT 
GGTGATGGTG TGAATGATGC TCCTGCTCTG AAGAAATCTG AAATCGGGAT TGCCATGGGC 
TCAGGGACTG CAGTGGCTAA GACTGCTTCT GAGATGGTCC TGGCAGATGA CAACTTCTCC 
ACCATTGTGG CTGCTGTTGA GGAGGGGCGA GCCATCTACA ACAACATGAA GCAGTTCATC 
CGCTACCTCA TCTCATCCAA CGTGGGGGAA GTGGTCTGTA TCTTCCTGAC GGCAGCCCTT 
GGGTTTCCTG AGGCTTTAAT TCCTGTCCAG TTACTCTGGG TCAATCTGGT GACAGATGGT 
CTGCCTGCCA CTGCGCTGGG GTTCAATCCT CCAGACCTGG ACATCATGAA CAAACCCCCC 
CGGAACCCAA AAGAACCACT GATCAGCGGG TGGCTCTTTT TCCGTTACCT GGCTATTGGC 
TGTTATGTTG GCGCTGCCAC CGTGGGTGCT GCTGCATGGT GGTTCATCGC TGCTGACGGC 
GGTCCAAGAG TCTCCTTCTA CCAGCTGAGT CATTTCCTAC AGTGTAAGGA GGACAACCCA 
GACTTCGATG GAGTGGATTG TGCAATCTTT GAGTCCCCAT ATCCAATGAC AATGGCACTT 
TCTGTTCTAG TAACCATAGA GATGTGTAAT GCCCTCAACA GCTTGTCTGA AAACCAGTCT 
TTGCTGAGGA TGCCCCCCTG GGAGAATATC TGGCTCGTGG GCTCCATCTG CTTGTCCATG 
TCACTTCACT TCTTGATCCT CTACGTGGAA CCTTTGCCGC TCATTTTCCA GATCACACCG 
CTGAATCTGA CCCAGTGGCT GATGGTGCTG AAAATCTCCT TGCCTGTGAT CCTCATGGAT 
GAGACGCTCA AGTTTGTGGC CCGAAACTAC CTGGAACAAC CCGCAATACT GGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033852.1
CDS	541..3537
Translation	MENAHTKTVEEVLGHFGVNESTGLSLEQVKKLKERWGSNELPAEEGKTLLELVIEQFEDLLVRILLLAACISFVLAWFEEGEETITAFVEPFVILLILVANAIVGVWQERNAENAIEALKEYEPEMGKVYRQDRKSVQRIKAKDIVPGDIVEIAVGDKVPADIRLTSIKSTTLRVDQSILTGESVSVIKHTDPVPDPRAVNQDKKNMLFSGTNIAAGKAMGVVVATGVNTEIGKIRDEMVATEQERTPLQQKLDEFGEQLSKVISLICIAVWIINIGHFNDPVHGGSWIRGAIYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVICSDKTGTLTTNQMSVCRMFILDKVEGDTCSLNEFSITGSTYAPIGEVQKDDKPVKCHQYDGLVELATICALCNDSALDYNEAKGVYEKVGEATETALTCLVEKMNVFDTELKGLSKIERANACNSVIKQLMKKEFTLEFSRDRKSMSVYCTPNKPSRTSMSKMFVKGAPEGVIDRCTHIRVGSTKVPMTPGVKQKIMSVIREWGSGSDTLRCLALATHDNPLKREEMHLEDSANFIKYETNLTFVGCVGMLDPPRIEVASSVKLCRQAGIRVIMITGDNKGTAVAICRRIGIFGQDEDVTSKAFTGREFDELSPSAQRDACLNARCFARVEPSHKSKIVEFLQSFDEITAMTGDGVNDAPALKKSEIGIAMGSGTAVAKTASEMVLADDNFSTIVAAVEEGRAIYNNMKQFIRYLISSNVGEVVCIFLTAALGFPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMNKPPRNPKEPLISGWLFFRYLAIGCYVGAATVGAAAWWFIAADGGPRVSFYQLSHFLQCKEDNPDFDGVDCAIFESPYPMTMALSVLVTIEMCNALNSLSENQSLLRMPPWENIWLVGSICLSMSLHFLILYVEPLPLIFQITPLNLTQWLMVLKISLPVILMDETLKFVARNYLEQPAILE

Target ORF information:

RefSeq Version	NM_009722.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 (Atp2a2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009722.3

ORF Insert Sequence:

ATGGAGAACG CTCACACAAA GACCGTGGAG GAGGTGCTGG GCCACTTCGG GGTCAACGAG 
AGCACGGGGC TGAGCTTGGA GCAGGTCAAG AAGCTCAAGG AGAGATGGGG CTCCAACGAA 
TTGCCGGCTG AAGAAGGAAA AACCTTGCTG GAACTTGTGA TTGAGCAGTT TGAAGACTTA 
CTAGTTAGAA TTTTACTGCT GGCAGCATGT ATATCTTTCG TTTTGGCTTG GTTCGAGGAA 
GGGGAAGAAA CGATTACAGC CTTTGTAGAG CCGTTTGTAA TTCTGCTTAT CTTGGTAGCC 
AATGCAATCG TGGGTGTGTG GCAGGAAAGA AATGCTGAAA ATGCAATAGA AGCTCTTAAG 
GAATATGAGC CTGAAATGGG CAAAGTGTAT CGACAGGACA GAAAGAGTGT GCAACGAATT 
AAAGCTAAAG ACATAGTTCC TGGTGATATA GTGGAAATTG CTGTTGGTGA CAAAGTTCCT 
GCTGATATTA GATTGACATC CATCAAGTCT ACAACTCTAA GAGTCGACCA GTCAATTCTT 
ACAGGTGAAT CTGTCTCCGT CATCAAGCAT ACTGACCCTG TCCCTGACCC CCGAGCTGTT 
AATCAAGACA AAAAGAACAT GCTCTTTTCT GGTACAAACA TTGCTGCTGG GAAAGCTATG 
GGAGTGGTGG TGGCAACTGG AGTTAATACT GAGATCGGCA AGATCCGGGA TGAAATGGTG 
GCAACAGAAC AGGAGAGAAC ACCCCTACAG CAGAAGCTAG ACGAGTTTGG GGAGCAGCTT 
TCCAAAGTTA TCTCCCTCAT TTGCATTGCA GTCTGGATCA TCAACATTGG GCATTTCAAT 
GACCCAGTTC ATGGTGGCTC CTGGATCAGG GGTGCCATCT ACTACTTTAA GATTGCCGTG 
GCCCTGGCTG TTGCCGCAAT CCCTGAGGGT CTGCCTGCTG TCATCACCAC CTGCTTAGCT 
CTTGGAACTC GTAGGATGGC AAAGAAAAAT GCTATCGTTC GAAGTCTGCC TTCTGTGGAG 
ACCCTTGGTT GTACTTCTGT TATCTGCTCA GATAAGACAG GCACACTTAC CACAAACCAG 
ATGTCCGTGT GCAGGATGTT CATTCTGGAC AAAGTAGAAG GTGACACTTG TTCCCTTAAT 
GAGTTCAGCA TAACTGGATC CACATATGCA CCAATTGGAG AAGTGCAAAA GGATGATAAG 
CCAGTGAAGT GCCATCAGTA TGACGGGCTT GTAGAGTTAG CCACCATCTG TGCTCTGTGT 
AATGACTCTG CTTTGGATTA TAATGAGGCA AAGGGTGTGT ATGAGAAAGT TGGAGAAGCT 
ACCGAGACTG CTCTCACGTG CCTGGTGGAG AAGATGAATG TATTTGATAC TGAGCTGAAG 
GGGCTTTCTA AAATAGAGCG TGCAAACGCC TGCAACTCGG TCATAAAGCA GCTGATGAAG 
AAGGAGTTCA CTCTGGAGTT TTCACGGGAT AGAAAATCAA TGTCCGTCTA TTGTACCCCA 
AACAAGCCAA GCCGGACATC CATGAGCAAG ATGTTTGTGA AGGGGGCTCC AGAAGGTGTC 
ATCGATAGGT GCACCCACAT CCGAGTTGGA AGTACCAAGG TCCCCATGAC TCCTGGTGTC 
AAACAGAAGA TTATGTCTGT CATTCGGGAG TGGGGCAGTG GCAGCGACAC GCTACGGTGC 
CTGGCTCTGG CCACTCATGA CAACCCACTG AAGAGAGAGG AGATGCACCT GGAAGACTCT 
GCTAACTTCA TCAAATACGA GACCAACCTG ACTTTCGTCG GCTGTGTGGG CATGCTGGAT 
CCTCCCAGGA TTGAAGTAGC CTCTTCTGTG AAGCTGTGCC GGCAAGCAGG CATCCGGGTC 
ATCATGATCA CTGGAGACAA CAAAGGCACC GCTGTGGCCA TCTGTCGCCG CATTGGCATC 
TTTGGGCAGG ATGAGGATGT GACATCAAAG GCTTTTACAG GGCGAGAGTT TGATGAATTA 
AGCCCTTCAG CCCAGAGAGA TGCCTGCTTA AATGCCCGCT GTTTTGCTCG AGTTGAACCT 
TCCCACAAGT CTAAGATTGT TGAGTTCCTT CAGTCCTTTG ATGAGATCAC AGCTATGACT 
GGTGATGGTG TGAATGATGC TCCTGCTCTG AAGAAATCTG AAATCGGGAT TGCCATGGGC 
TCAGGGACTG CAGTGGCTAA GACTGCTTCT GAGATGGTCC TGGCAGATGA CAACTTCTCC 
ACCATTGTGG CTGCTGTTGA GGAGGGGCGA GCCATCTACA ACAACATGAA GCAGTTCATC 
CGCTACCTCA TCTCATCCAA CGTGGGGGAA GTGGTCTGTA TCTTCCTGAC GGCAGCCCTT 
GGGTTTCCTG AGGCTTTAAT TCCTGTCCAG TTACTCTGGG TCAATCTGGT GACAGATGGT 
CTGCCTGCCA CTGCGCTGGG GTTCAATCCT CCAGACCTGG ACATCATGAA CAAACCCCCC 
CGGAACCCAA AAGAACCACT GATCAGCGGG TGGCTCTTTT TCCGTTACCT GGCTATTGGC 
TGTTATGTTG GCGCTGCCAC CGTGGGTGCT GCTGCATGGT GGTTCATCGC TGCTGACGGC 
GGTCCAAGAG TCTCCTTCTA CCAGCTGAGT CATTTCCTAC AGTGTAAGGA GGACAACCCA 
GACTTCGATG GAGTGGATTG TGCAATCTTT GAGTCCCCAT ATCCAATGAC AATGGCACTT 
TCTGTTCTAG TAACCATAGA GATGTGTAAT GCCCTCAACA GCTTGTCTGA AAACCAGTCT 
TTGCTGAGGA TGCCCCCCTG GGAGAATATC TGGCTCGTGG GCTCCATCTG CTTGTCCATG 
TCACTTCACT TCTTGATCCT CTACGTGGAA CCTTTGCCGC TCATTTTCCA GATCACACCG 
CTGAATCTGA CCCAGTGGCT GATGGTGCTG AAAATCTCCT TGCCTGTGAT CCTCATGGAT 
GAGACGCTCA AGTTTGTGGC CCGAAACTAC CTGGAACAAC CCGCAATACT GGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atp2a2 ( NM_001110140.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001103610.1 Mus musculus ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 (Atp2a2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu19022
Clone ID Related Accession (Same CDS sequence)	NM_001110140.3
Accession Version	NM_001110140.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	sarcoplasmic/endoplasmic reticulum calcium ATPase 2 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ162528.1, AK220538.1, AJ131821.1 and CF742600.1. On Jun 20, 2009 this sequence version replaced NM_001110140.2. Transcript Variant: This variant (1) encodes the longer isoform (b), also known as SERCA2B. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ131821.1, SRR1660817.66707.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAACG CTCACACAAA GACCGTGGAG GAGGTGCTGG GCCACTTCGG GGTCAACGAG 
AGCACGGGGC TGAGCTTGGA GCAGGTCAAG AAGCTCAAGG AGAGATGGGG CTCCAACGAA 
TTGCCGGCTG AAGAAGGAAA AACCTTGCTG GAACTTGTGA TTGAGCAGTT TGAAGACTTA 
CTAGTTAGAA TTTTACTGCT GGCAGCATGT ATATCTTTCG TTTTGGCTTG GTTCGAGGAA 
GGGGAAGAAA CGATTACAGC CTTTGTAGAG CCGTTTGTAA TTCTGCTTAT CTTGGTAGCC 
AATGCAATCG TGGGTGTGTG GCAGGAAAGA AATGCTGAAA ATGCAATAGA AGCTCTTAAG 
GAATATGAGC CTGAAATGGG CAAAGTGTAT CGACAGGACA GAAAGAGTGT GCAACGAATT 
AAAGCTAAAG ACATAGTTCC TGGTGATATA GTGGAAATTG CTGTTGGTGA CAAAGTTCCT 
GCTGATATTA GATTGACATC CATCAAGTCT ACAACTCTAA GAGTCGACCA GTCAATTCTT 
ACAGGTGAAT CTGTCTCCGT CATCAAGCAT ACTGACCCTG TCCCTGACCC CCGAGCTGTT 
AATCAAGACA AAAAGAACAT GCTCTTTTCT GGTACAAACA TTGCTGCTGG GAAAGCTATG 
GGAGTGGTGG TGGCAACTGG AGTTAATACT GAGATCGGCA AGATCCGGGA TGAAATGGTG 
GCAACAGAAC AGGAGAGAAC ACCCCTACAG CAGAAGCTAG ACGAGTTTGG GGAGCAGCTT 
TCCAAAGTTA TCTCCCTCAT TTGCATTGCA GTCTGGATCA TCAACATTGG GCATTTCAAT 
GACCCAGTTC ATGGTGGCTC CTGGATCAGG GGTGCCATCT ACTACTTTAA GATTGCCGTG 
GCCCTGGCTG TTGCCGCAAT CCCTGAGGGT CTGCCTGCTG TCATCACCAC CTGCTTAGCT 
CTTGGAACTC GTAGGATGGC AAAGAAAAAT GCTATCGTTC GAAGTCTGCC TTCTGTGGAG 
ACCCTTGGTT GTACTTCTGT TATCTGCTCA GATAAGACAG GCACACTTAC CACAAACCAG 
ATGTCCGTGT GCAGGATGTT CATTCTGGAC AAAGTAGAAG GTGACACTTG TTCCCTTAAT 
GAGTTCAGCA TAACTGGATC CACATATGCA CCAATTGGAG AAGTGCAAAA GGATGATAAG 
CCAGTGAAGT GCCATCAGTA TGACGGGCTT GTAGAGTTAG CCACCATCTG TGCTCTGTGT 
AATGACTCTG CTTTGGATTA TAATGAGGCA AAGGGTGTGT ATGAGAAAGT TGGAGAAGCT 
ACCGAGACTG CTCTCACGTG CCTGGTGGAG AAGATGAATG TATTTGATAC TGAGCTGAAG 
GGGCTTTCTA AAATAGAGCG TGCAAACGCC TGCAACTCGG TCATAAAGCA GCTGATGAAG 
AAGGAGTTCA CTCTGGAGTT TTCACGGGAT AGAAAATCAA TGTCCGTCTA TTGTACCCCA 
AACAAGCCAA GCCGGACATC CATGAGCAAG ATGTTTGTGA AGGGGGCTCC AGAAGGTGTC 
ATCGATAGGT GCACCCACAT CCGAGTTGGA AGTACCAAGG TCCCCATGAC TCCTGGTGTC 
AAACAGAAGA TTATGTCTGT CATTCGGGAG TGGGGCAGTG GCAGCGACAC GCTACGGTGC 
CTGGCTCTGG CCACTCATGA CAACCCACTG AAGAGAGAGG AGATGCACCT GGAAGACTCT 
GCTAACTTCA TCAAATACGA GACCAACCTG ACTTTCGTCG GCTGTGTGGG CATGCTGGAT 
CCTCCCAGGA TTGAAGTAGC CTCTTCTGTG AAGCTGTGCC GGCAAGCAGG CATCCGGGTC 
ATCATGATCA CTGGAGACAA CAAAGGCACC GCTGTGGCCA TCTGTCGCCG CATTGGCATC 
TTTGGGCAGG ATGAGGATGT GACATCAAAG GCTTTTACAG GGCGAGAGTT TGATGAATTA 
AGCCCTTCAG CCCAGAGAGA TGCCTGCTTA AATGCCCGCT GTTTTGCTCG AGTTGAACCT 
TCCCACAAGT CTAAGATTGT TGAGTTCCTT CAGTCCTTTG ATGAGATCAC AGCTATGACT 
GGTGATGGTG TGAATGATGC TCCTGCTCTG AAGAAATCTG AAATCGGGAT TGCCATGGGC 
TCAGGGACTG CAGTGGCTAA GACTGCTTCT GAGATGGTCC TGGCAGATGA CAACTTCTCC 
ACCATTGTGG CTGCTGTTGA GGAGGGGCGA GCCATCTACA ACAACATGAA GCAGTTCATC 
CGCTACCTCA TCTCATCCAA CGTGGGGGAA GTGGTCTGTA TCTTCCTGAC GGCAGCCCTT 
GGGTTTCCTG AGGCTTTAAT TCCTGTCCAG TTACTCTGGG TCAATCTGGT GACAGATGGT 
CTGCCTGCCA CTGCGCTGGG GTTCAATCCT CCAGACCTGG ACATCATGAA CAAACCCCCC 
CGGAACCCAA AAGAACCACT GATCAGCGGG TGGCTCTTTT TCCGTTACCT GGCTATTGGC 
TGTTATGTTG GCGCTGCCAC CGTGGGTGCT GCTGCATGGT GGTTCATCGC TGCTGACGGC 
GGTCCAAGAG TCTCCTTCTA CCAGCTGAGT CATTTCCTAC AGTGTAAGGA GGACAACCCA 
GACTTCGATG GAGTGGATTG TGCAATCTTT GAGTCCCCAT ATCCAATGAC AATGGCACTT 
TCTGTTCTAG TAACCATAGA GATGTGTAAT GCCCTCAACA GCTTGTCTGA AAACCAGTCT 
TTGCTGAGGA TGCCCCCCTG GGAGAATATC TGGCTCGTGG GCTCCATCTG CTTGTCCATG 
TCACTTCACT TCTTGATCCT CTACGTGGAA CCTTTGCCGC TCATTTTCCA GATCACACCG 
CTGAATCTGA CCCAGTGGCT GATGGTGCTG AAAATCTCCT TGCCTGTGAT CCTCATGGAT 
GAGACGCTCA AGTTTGTGGC CCGAAACTAC CTGGAACAAC CCGGTAAAGA GTGTGTGCAG 
CCTGCCACCA AATCTTCCTG CTCCCTGTCG GCATGCACCG ATGGCATTTC CTGGCCGTTT 
GTGCTGCTCA TTATGCCCCT GGTGGTCTGG GTCTACAGCA CAGACACTAA CTTTAGCGAT 
ATGTTCTGGT CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001103610.1
CDS	541..3675
Translation	MENAHTKTVEEVLGHFGVNESTGLSLEQVKKLKERWGSNELPAEEGKTLLELVIEQFEDLLVRILLLAACISFVLAWFEEGEETITAFVEPFVILLILVANAIVGVWQERNAENAIEALKEYEPEMGKVYRQDRKSVQRIKAKDIVPGDIVEIAVGDKVPADIRLTSIKSTTLRVDQSILTGESVSVIKHTDPVPDPRAVNQDKKNMLFSGTNIAAGKAMGVVVATGVNTEIGKIRDEMVATEQERTPLQQKLDEFGEQLSKVISLICIAVWIINIGHFNDPVHGGSWIRGAIYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVICSDKTGTLTTNQMSVCRMFILDKVEGDTCSLNEFSITGSTYAPIGEVQKDDKPVKCHQYDGLVELATICALCNDSALDYNEAKGVYEKVGEATETALTCLVEKMNVFDTELKGLSKIERANACNSVIKQLMKKEFTLEFSRDRKSMSVYCTPNKPSRTSMSKMFVKGAPEGVIDRCTHIRVGSTKVPMTPGVKQKIMSVIREWGSGSDTLRCLALATHDNPLKREEMHLEDSANFIKYETNLTFVGCVGMLDPPRIEVASSVKLCRQAGIRVIMITGDNKGTAVAICRRIGIFGQDEDVTSKAFTGREFDELSPSAQRDACLNARCFARVEPSHKSKIVEFLQSFDEITAMTGDGVNDAPALKKSEIGIAMGSGTAVAKTASEMVLADDNFSTIVAAVEEGRAIYNNMKQFIRYLISSNVGEVVCIFLTAALGFPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMNKPPRNPKEPLISGWLFFRYLAIGCYVGAATVGAAAWWFIAADGGPRVSFYQLSHFLQCKEDNPDFDGVDCAIFESPYPMTMALSVLVTIEMCNALNSLSENQSLLRMPPWENIWLVGSICLSMSLHFLILYVEPLPLIFQITPLNLTQWLMVLKISLPVILMDETLKFVARNYLEQPGKECVQPATKSSCSLSACTDGISWPFVLLIMPLVVWVYSTDTNFSDMFWS

Target ORF information:

RefSeq Version	NM_001110140.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 (Atp2a2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001110140.3

ORF Insert Sequence:

ATGGAGAACG CTCACACAAA GACCGTGGAG GAGGTGCTGG GCCACTTCGG GGTCAACGAG 
AGCACGGGGC TGAGCTTGGA GCAGGTCAAG AAGCTCAAGG AGAGATGGGG CTCCAACGAA 
TTGCCGGCTG AAGAAGGAAA AACCTTGCTG GAACTTGTGA TTGAGCAGTT TGAAGACTTA 
CTAGTTAGAA TTTTACTGCT GGCAGCATGT ATATCTTTCG TTTTGGCTTG GTTCGAGGAA 
GGGGAAGAAA CGATTACAGC CTTTGTAGAG CCGTTTGTAA TTCTGCTTAT CTTGGTAGCC 
AATGCAATCG TGGGTGTGTG GCAGGAAAGA AATGCTGAAA ATGCAATAGA AGCTCTTAAG 
GAATATGAGC CTGAAATGGG CAAAGTGTAT CGACAGGACA GAAAGAGTGT GCAACGAATT 
AAAGCTAAAG ACATAGTTCC TGGTGATATA GTGGAAATTG CTGTTGGTGA CAAAGTTCCT 
GCTGATATTA GATTGACATC CATCAAGTCT ACAACTCTAA GAGTCGACCA GTCAATTCTT 
ACAGGTGAAT CTGTCTCCGT CATCAAGCAT ACTGACCCTG TCCCTGACCC CCGAGCTGTT 
AATCAAGACA AAAAGAACAT GCTCTTTTCT GGTACAAACA TTGCTGCTGG GAAAGCTATG 
GGAGTGGTGG TGGCAACTGG AGTTAATACT GAGATCGGCA AGATCCGGGA TGAAATGGTG 
GCAACAGAAC AGGAGAGAAC ACCCCTACAG CAGAAGCTAG ACGAGTTTGG GGAGCAGCTT 
TCCAAAGTTA TCTCCCTCAT TTGCATTGCA GTCTGGATCA TCAACATTGG GCATTTCAAT 
GACCCAGTTC ATGGTGGCTC CTGGATCAGG GGTGCCATCT ACTACTTTAA GATTGCCGTG 
GCCCTGGCTG TTGCCGCAAT CCCTGAGGGT CTGCCTGCTG TCATCACCAC CTGCTTAGCT 
CTTGGAACTC GTAGGATGGC AAAGAAAAAT GCTATCGTTC GAAGTCTGCC TTCTGTGGAG 
ACCCTTGGTT GTACTTCTGT TATCTGCTCA GATAAGACAG GCACACTTAC CACAAACCAG 
ATGTCCGTGT GCAGGATGTT CATTCTGGAC AAAGTAGAAG GTGACACTTG TTCCCTTAAT 
GAGTTCAGCA TAACTGGATC CACATATGCA CCAATTGGAG AAGTGCAAAA GGATGATAAG 
CCAGTGAAGT GCCATCAGTA TGACGGGCTT GTAGAGTTAG CCACCATCTG TGCTCTGTGT 
AATGACTCTG CTTTGGATTA TAATGAGGCA AAGGGTGTGT ATGAGAAAGT TGGAGAAGCT 
ACCGAGACTG CTCTCACGTG CCTGGTGGAG AAGATGAATG TATTTGATAC TGAGCTGAAG 
GGGCTTTCTA AAATAGAGCG TGCAAACGCC TGCAACTCGG TCATAAAGCA GCTGATGAAG 
AAGGAGTTCA CTCTGGAGTT TTCACGGGAT AGAAAATCAA TGTCCGTCTA TTGTACCCCA 
AACAAGCCAA GCCGGACATC CATGAGCAAG ATGTTTGTGA AGGGGGCTCC AGAAGGTGTC 
ATCGATAGGT GCACCCACAT CCGAGTTGGA AGTACCAAGG TCCCCATGAC TCCTGGTGTC 
AAACAGAAGA TTATGTCTGT CATTCGGGAG TGGGGCAGTG GCAGCGACAC GCTACGGTGC 
CTGGCTCTGG CCACTCATGA CAACCCACTG AAGAGAGAGG AGATGCACCT GGAAGACTCT 
GCTAACTTCA TCAAATACGA GACCAACCTG ACTTTCGTCG GCTGTGTGGG CATGCTGGAT 
CCTCCCAGGA TTGAAGTAGC CTCTTCTGTG AAGCTGTGCC GGCAAGCAGG CATCCGGGTC 
ATCATGATCA CTGGAGACAA CAAAGGCACC GCTGTGGCCA TCTGTCGCCG CATTGGCATC 
TTTGGGCAGG ATGAGGATGT GACATCAAAG GCTTTTACAG GGCGAGAGTT TGATGAATTA 
AGCCCTTCAG CCCAGAGAGA TGCCTGCTTA AATGCCCGCT GTTTTGCTCG AGTTGAACCT 
TCCCACAAGT CTAAGATTGT TGAGTTCCTT CAGTCCTTTG ATGAGATCAC AGCTATGACT 
GGTGATGGTG TGAATGATGC TCCTGCTCTG AAGAAATCTG AAATCGGGAT TGCCATGGGC 
TCAGGGACTG CAGTGGCTAA GACTGCTTCT GAGATGGTCC TGGCAGATGA CAACTTCTCC 
ACCATTGTGG CTGCTGTTGA GGAGGGGCGA GCCATCTACA ACAACATGAA GCAGTTCATC 
CGCTACCTCA TCTCATCCAA CGTGGGGGAA GTGGTCTGTA TCTTCCTGAC GGCAGCCCTT 
GGGTTTCCTG AGGCTTTAAT TCCTGTCCAG TTACTCTGGG TCAATCTGGT GACAGATGGT 
CTGCCTGCCA CTGCGCTGGG GTTCAATCCT CCAGACCTGG ACATCATGAA CAAACCCCCC 
CGGAACCCAA AAGAACCACT GATCAGCGGG TGGCTCTTTT TCCGTTACCT GGCTATTGGC 
TGTTATGTTG GCGCTGCCAC CGTGGGTGCT GCTGCATGGT GGTTCATCGC TGCTGACGGC 
GGTCCAAGAG TCTCCTTCTA CCAGCTGAGT CATTTCCTAC AGTGTAAGGA GGACAACCCA 
GACTTCGATG GAGTGGATTG TGCAATCTTT GAGTCCCCAT ATCCAATGAC AATGGCACTT 
TCTGTTCTAG TAACCATAGA GATGTGTAAT GCCCTCAACA GCTTGTCTGA AAACCAGTCT 
TTGCTGAGGA TGCCCCCCTG GGAGAATATC TGGCTCGTGG GCTCCATCTG CTTGTCCATG 
TCACTTCACT TCTTGATCCT CTACGTGGAA CCTTTGCCGC TCATTTTCCA GATCACACCG 
CTGAATCTGA CCCAGTGGCT GATGGTGCTG AAAATCTCCT TGCCTGTGAT CCTCATGGAT 
GAGACGCTCA AGTTTGTGGC CCGAAACTAC CTGGAACAAC CCGGTAAAGA GTGTGTGCAG 
CCTGCCACCA AATCTTCCTG CTCCCTGTCG GCATGCACCG ATGGCATTTC CTGGCCGTTT 
GTGCTGCTCA TTATGCCCCT GGTGGTCTGG GTCTACAGCA CAGACACTAA CTTTAGCGAT 
ATGTTCTGGT CTTGA