Rhod cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rhod
Entrez Gene ID	11854
Full Name	ras homolog family member D
Synonyms	AI326383,Arhd,Rho,RhoHP1,RhoM
General protein information	Preferred Names ras homolog family member D Names rho-related GTP-binding protein RhoD aplysia ras-related homolog D ras homolog D (RhoD) ras homolog gene family, member D
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19\|19 A

mRNA and Protein(s)

mRNA	Protein	Name
NM_007485.5	NP_031511.1	rho-related GTP-binding protein RhoD isoform 1
XM_006531644.2	XP_006531707.1	rho-related GTP-binding protein RhoD isoform X1
NM_001329989.1	NP_001316918.1	rho-related GTP-binding protein RhoD isoform 2
XM_006531644.3	XP_006531707.1	rho-related GTP-binding protein RhoD isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Rhod	NP_031511.1
Pan troglodytes (chimpanzee)	RHOD	XP_508584.2
	LOC712969	XP_001107631.2
Bos taurus (cattle)	RHOD	NP_001179267.1
Homo sapiens (human)	RHOD	NP_055393.1
Canis lupus familiaris (dog)	RHOD	XP_854676.3
Rattus norvegicus (Norway rat)	Rhod	NP_001099793.1

Gene Ontology
Bibliography

Related articles in PubMed

RhoD is a Golgi component with a role in anterograde protein transport from the ER to the plasma membrane.
Blom M, Reis K, Nehru V, Blom H, Gad AK, Aspenstr?m P
Experimental cell research333(2)208-19(2015 May)

Atypical Rho GTPases RhoD and Rif integrate cytoskeletal dynamics and membrane trafficking.
Aspenstr?m P
Biological chemistry395(5)477-84(2014 May)

RhoD binds the Rab5 effector Rabankyrin-5 and has a role in trafficking of the platelet-derived growth factor receptor.
Nehru V, Voytyuk O, Lennartsson J, Aspenstr?m P
Traffic (Copenhagen, Denmark)14(12)1242-54(2013 Dec)

Rif proteins take to the RhoD: Rho GTPases at the crossroads of actin dynamics and membrane trafficking.
Gad AK, Aspenstr?m P
Cellular signalling22(2)183-9(2010 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

RhoD was activated by FGF signaling, and RhoD-activated mDia3C was responsible for the actin filament formation in the protrusion.
Title: RhoD activated by fibroblast growth factor induces cytoneme-like cellular protrusions through mDia3C.

Lysophosphatidic acid signals through LPA2 receptors and Galphaq proteins of cultured proximal tubule cells to transactivate latent TGF-beta in a Rho/Rho-kinase and alphavbeta6 integrin-dependent manner.
Title: Lysophosphatidic acid increases proximal tubule cell secretion of profibrotic cytokines PDGF-B and CTGF through LPA2- and G?q-mediated Rho and ?v?6 integrin-dependent activation of TGF-?.

Podocyte BK(Ca) channels are regulated by synaptopodin, Rho, and actin microfilaments.
Title: Regulation of podocyte BK(Ca) channels by synaptopodin, Rho, and actin microfilaments.

ERK5 promotes Src-induced podosome formation by inducing RhoGAP7 and thereby limiting Rho activation.
Title: ERK5 promotes Src-induced podosome formation by limiting Rho activation.

The following Rhod gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rhod cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu20277	NM_007485.5 Latest version!	Mus musculus ras homolog family member D (Rhod), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OMu83504	NM_001329989.1 Latest version!	Mus musculus ras homolog family member D (Rhod), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu55007	XM_006531644.3 Latest version!	Mus musculus ras homolog family member D (Rhod), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OMu55007	XM_006531644.2	Mus musculus ras homolog family member D (Rhod), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rhod ( NM_007485.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031511.1 Mus musculus ras homolog family member D (Rhod), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu20277
Clone ID Related Accession (Same CDS sequence)	NM_007485.5
Accession Version	NM_007485.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	rho-related GTP-binding protein RhoD isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC124347.4, AK008880.1 and BX516716.1. On Aug 12, 2016 this sequence version replaced NM_007485.4. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK008880.1, D89821.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164140 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACGCGT CCCAGGTTGC GGGAGAAGAG GCGCCGCAGA GCGGGCACTC GGTCAAGGTG 
GTCCTGGTGG GCGACGGGGG CTGCGGGAAG ACGTCACTGA TGATGGTCTT CGCCAAAGGG 
GCCTTCCCAG AGAGCTACAG TCCCACAGTG TTTGAGCGCT ATAATGCCAC TCTGCAGATG 
AAGGGTAAAC CTGTGCACCT CCAAATCTGG GACACAGCCG GGCAAGATGA CTATGACCGC 
CTCCGGCCCT TGTTCTATCC TGATGCCAAT GTCTTGCTCC TCTGCTTCGA TGTGACCAAT 
CCAAACAGCT TTGACAACGT CTCCAACCGG TGGTACCCAG AGGTGACACA TTTCTGCAAG 
GGAGTGCCCA TCATTGTTGT GGGCTGCAAG ATAGACCTGC GTAAGGACAA GGTGCTGGTG 
AACAACCTGC GGAAGAAAAG ACTGGAGCCC GTGACCTACC ACAGGGGCCA CGATATGGCA 
AGGTCTGTGG GAGCGGTGGC CTATCTTGAG TGTTCAGCTC GGCTCCATGA CAACGTGGAA 
GCCGTCTTCC AGGAAGCAGC AGAAGTGGCT CTCAGCAGTC GCAGACATAA CTTTTGGCGG 
CGGATTACTC AGAATTGTTG CTTGGCCACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031511.1
CDS	93..725
Translation	MNASQVAGEEAPQSGHSVKVVLVGDGGCGKTSLMMVFAKGAFPESYSPTVFERYNATLQMKGKPVHLQIWDTAGQDDYDRLRPLFYPDANVLLLCFDVTNPNSFDNVSNRWYPEVTHFCKGVPIIVVGCKIDLRKDKVLVNNLRKKRLEPVTYHRGHDMARSVGAVAYLECSARLHDNVEAVFQEAAEVALSSRRHNFWRRITQNCCLAT

Target ORF information:

RefSeq Version	NM_007485.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus ras homolog family member D (Rhod), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007485.5

ORF Insert Sequence:

ATGAACGCGT CCCAGGTTGC GGGAGAAGAG GCGCCGCAGA GCGGGCACTC GGTCAAGGTG 
GTCCTGGTGG GCGACGGGGG CTGCGGGAAG ACGTCACTGA TGATGGTCTT CGCCAAAGGG 
GCCTTCCCAG AGAGCTACAG TCCCACAGTG TTTGAGCGCT ATAATGCCAC TCTGCAGATG 
AAGGGTAAAC CTGTGCACCT CCAAATCTGG GACACAGCCG GGCAAGATGA CTATGACCGC 
CTCCGGCCCT TGTTCTATCC TGATGCCAAT GTCTTGCTCC TCTGCTTCGA TGTGACCAAT 
CCAAACAGCT TTGACAACGT CTCCAACCGG TGGTACCCAG AGGTGACACA TTTCTGCAAG 
GGAGTGCCCA TCATTGTTGT GGGCTGCAAG ATAGACCTGC GTAAGGACAA GGTGCTGGTG 
AACAACCTGC GGAAGAAAAG ACTGGAGCCC GTGACCTACC ACAGGGGCCA CGATATGGCA 
AGGTCTGTGG GAGCGGTGGC CTATCTTGAG TGTTCAGCTC GGCTCCATGA CAACGTGGAA 
GCCGTCTTCC AGGAAGCAGC AGAAGTGGCT CTCAGCAGTC GCAGACATAA CTTTTGGCGG 
CGGATTACTC AGAATTGTTG CTTGGCCACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rhod ( XM_006531644.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006531707.1 Mus musculus ras homolog family member D (Rhod), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu55007
Clone ID Related Accession (Same CDS sequence)	XM_006531644.2 , XM_006531644.3
Accession Version	XM_006531644.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	rho-related GTP-binding protein RhoD isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006531644.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGCGT CCCAGGTTGC GGGAGAAGAG GCGCCGCAGA GCGGGCACTC GGTCAAGGTG 
GTCCTGGTGG GCGACGGGGG CTGCGGGAAG ACGTCACTGA TGATGGTCTT CGCCAAAGGG 
GCCTTCCCAG AGAGCTACAG TCCCACAGTG TTTGAGCGCT ATAATGCCAC TCTGCAGATG 
AAGGGTAAAC CTGTGCACCT CCAAATCTGG GACACAGCCG GGCAAGATGA CTATGACCGC 
CTCCGGCCCT TGTTCTATCC TGATGCCAAT GTCTTGCTCC TCTGCTTCGA TGTGACCAAT 
CCAAACAGCT TTGACAACGT CTCCAACCGG TGGTACCCAG AGGTGACACA TTTCTGCAAG 
GGAGTGCCCA TCATTGTTGT GGGCTGCAAG ATAGACCTGC GTAAGGACAA GGTGCTGGTG 
AACAACCTGC GGAAGAAAAG ACTGGAGCCC GTGACCTACC ACAGGGTAGG TAGGAAGCTA 
GGCAACCTCT GGATACTTCC CCACTGTGCC AGGTGTGTCG CACACGGGGG TCTTGGCGGA 
TGTCAGCAGT CAGGAAGGGG CAGCACTTGG AAGCAGAACC CAGAATGCTC AAGGAGGCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006531707.1
CDS	44..646
Translation	MNASQVAGEEAPQSGHSVKVVLVGDGGCGKTSLMMVFAKGAFPESYSPTVFERYNATLQMKGKPVHLQIWDTAGQDDYDRLRPLFYPDANVLLLCFDVTNPNSFDNVSNRWYPEVTHFCKGVPIIVVGCKIDLRKDKVLVNNLRKKRLEPVTYHRVGRKLGNLWILPHCARCVAHGGLGGCQQSGRGSTWKQNPECSRRL

Target ORF information:

RefSeq Version	XM_006531644.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ras homolog family member D (Rhod), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006531644.2

ORF Insert Sequence:

ATGAACGCGT CCCAGGTTGC GGGAGAAGAG GCGCCGCAGA GCGGGCACTC GGTCAAGGTG 
GTCCTGGTGG GCGACGGGGG CTGCGGGAAG ACGTCACTGA TGATGGTCTT CGCCAAAGGG 
GCCTTCCCAG AGAGCTACAG TCCCACAGTG TTTGAGCGCT ATAATGCCAC TCTGCAGATG 
AAGGGTAAAC CTGTGCACCT CCAAATCTGG GACACAGCCG GGCAAGATGA CTATGACCGC 
CTCCGGCCCT TGTTCTATCC TGATGCCAAT GTCTTGCTCC TCTGCTTCGA TGTGACCAAT 
CCAAACAGCT TTGACAACGT CTCCAACCGG TGGTACCCAG AGGTGACACA TTTCTGCAAG 
GGAGTGCCCA TCATTGTTGT GGGCTGCAAG ATAGACCTGC GTAAGGACAA GGTGCTGGTG 
AACAACCTGC GGAAGAAAAG ACTGGAGCCC GTGACCTACC ACAGGGTAGG TAGGAAGCTA 
GGCAACCTCT GGATACTTCC CCACTGTGCC AGGTGTGTCG CACACGGGGG TCTTGGCGGA 
TGTCAGCAGT CAGGAAGGGG CAGCACTTGG AAGCAGAACC CAGAATGCTC AAGGAGGCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rhod ( NM_001329989.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001316918.1 Mus musculus ras homolog family member D (Rhod), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu83504
Clone ID Related Accession (Same CDS sequence)	NM_001329989.1
Accession Version	NM_001329989.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	rho-related GTP-binding protein RhoD isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC124347.4, AK008880.1 and BX516716.1. Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. It encodes isoform 2, which has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DT917327.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164140 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACGCGT CCCAGGTTGC GGGAGAAGAG GCGCCGCAGA GCGGGCACTC GGTCAAGGTG 
GTCCTGGTGG GCGACGGGGG CTGCGGGAAG ACGTCACTGA TGATGGTCTT CGCCAAAGGG 
GCCTTCCCAG AGAGCTACAG TCCCACAGTG TTTGAGCGCT ATAATGCCAC TCTGCAGATG 
AAGGGTAAAC CTGTGCACCT CCAAATCTGG GACACAGCCG GGCAAGATGA CTATGACCGC 
CTCCGGCCCT TGTTCTATCC TGATGCCAAT GTCTTGCTCC TCTGCTTCGA TGTGACCAAT 
CCAAACAGCT TTGACAACGT CTCCAACCGG TGGTACCCAG AGGTGACACA TTTCTGCAAG 
GGAGTGCCCA TCATTGTTGT GGGCTGCAAG ATAGACCTGC GTAAGGACAA GGTGCTGGTG 
AACAACCTGC GGAAGAAAAG ACTGGAGCCC GTGACCTACC ACAGGGTAGG GCCACGATAT 
GGCAAGGTCT GTGGGAGCGG TGGCCTATCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001316918.1
CDS	93..605
Translation	MNASQVAGEEAPQSGHSVKVVLVGDGGCGKTSLMMVFAKGAFPESYSPTVFERYNATLQMKGKPVHLQIWDTAGQDDYDRLRPLFYPDANVLLLCFDVTNPNSFDNVSNRWYPEVTHFCKGVPIIVVGCKIDLRKDKVLVNNLRKKRLEPVTYHRVGPRYGKVCGSGGLS

Target ORF information:

RefSeq Version	NM_001329989.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ras homolog family member D (Rhod), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001329989.1

ORF Insert Sequence:

ATGAACGCGT CCCAGGTTGC GGGAGAAGAG GCGCCGCAGA GCGGGCACTC GGTCAAGGTG 
GTCCTGGTGG GCGACGGGGG CTGCGGGAAG ACGTCACTGA TGATGGTCTT CGCCAAAGGG 
GCCTTCCCAG AGAGCTACAG TCCCACAGTG TTTGAGCGCT ATAATGCCAC TCTGCAGATG 
AAGGGTAAAC CTGTGCACCT CCAAATCTGG GACACAGCCG GGCAAGATGA CTATGACCGC 
CTCCGGCCCT TGTTCTATCC TGATGCCAAT GTCTTGCTCC TCTGCTTCGA TGTGACCAAT 
CCAAACAGCT TTGACAACGT CTCCAACCGG TGGTACCCAG AGGTGACACA TTTCTGCAAG 
GGAGTGCCCA TCATTGTTGT GGGCTGCAAG ATAGACCTGC GTAAGGACAA GGTGCTGGTG 
AACAACCTGC GGAAGAAAAG ACTGGAGCCC GTGACCTACC ACAGGGTAGG GCCACGATAT 
GGCAAGGTCT GTGGGAGCGG TGGCCTATCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rhod ( XM_006531644.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006531707.1 Mus musculus ras homolog family member D (Rhod), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu55007
Clone ID Related Accession (Same CDS sequence)	XM_006531644.2 , XM_006531644.3
Accession Version	XM_006531644.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rho-related GTP-binding protein RhoD isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006531644.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGCGT CCCAGGTTGC GGGAGAAGAG GCGCCGCAGA GCGGGCACTC GGTCAAGGTG 
GTCCTGGTGG GCGACGGGGG CTGCGGGAAG ACGTCACTGA TGATGGTCTT CGCCAAAGGG 
GCCTTCCCAG AGAGCTACAG TCCCACAGTG TTTGAGCGCT ATAATGCCAC TCTGCAGATG 
AAGGGTAAAC CTGTGCACCT CCAAATCTGG GACACAGCCG GGCAAGATGA CTATGACCGC 
CTCCGGCCCT TGTTCTATCC TGATGCCAAT GTCTTGCTCC TCTGCTTCGA TGTGACCAAT 
CCAAACAGCT TTGACAACGT CTCCAACCGG TGGTACCCAG AGGTGACACA TTTCTGCAAG 
GGAGTGCCCA TCATTGTTGT GGGCTGCAAG ATAGACCTGC GTAAGGACAA GGTGCTGGTG 
AACAACCTGC GGAAGAAAAG ACTGGAGCCC GTGACCTACC ACAGGGTAGG TAGGAAGCTA 
GGCAACCTCT GGATACTTCC CCACTGTGCC AGGTGTGTCG CACACGGGGG TCTTGGCGGA 
TGTCAGCAGT CAGGAAGGGG CAGCACTTGG AAGCAGAACC CAGAATGCTC AAGGAGGCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006531707.1
CDS	184..786
Translation	MNASQVAGEEAPQSGHSVKVVLVGDGGCGKTSLMMVFAKGAFPESYSPTVFERYNATLQMKGKPVHLQIWDTAGQDDYDRLRPLFYPDANVLLLCFDVTNPNSFDNVSNRWYPEVTHFCKGVPIIVVGCKIDLRKDKVLVNNLRKKRLEPVTYHRVGRKLGNLWILPHCARCVAHGGLGGCQQSGRGSTWKQNPECSRRL

Target ORF information:

RefSeq Version	XM_006531644.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ras homolog family member D (Rhod), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006531644.3

ORF Insert Sequence:

ATGAACGCGT CCCAGGTTGC GGGAGAAGAG GCGCCGCAGA GCGGGCACTC GGTCAAGGTG 
GTCCTGGTGG GCGACGGGGG CTGCGGGAAG ACGTCACTGA TGATGGTCTT CGCCAAAGGG 
GCCTTCCCAG AGAGCTACAG TCCCACAGTG TTTGAGCGCT ATAATGCCAC TCTGCAGATG 
AAGGGTAAAC CTGTGCACCT CCAAATCTGG GACACAGCCG GGCAAGATGA CTATGACCGC 
CTCCGGCCCT TGTTCTATCC TGATGCCAAT GTCTTGCTCC TCTGCTTCGA TGTGACCAAT 
CCAAACAGCT TTGACAACGT CTCCAACCGG TGGTACCCAG AGGTGACACA TTTCTGCAAG 
GGAGTGCCCA TCATTGTTGT GGGCTGCAAG ATAGACCTGC GTAAGGACAA GGTGCTGGTG 
AACAACCTGC GGAAGAAAAG ACTGGAGCCC GTGACCTACC ACAGGGTAGG TAGGAAGCTA 
GGCAACCTCT GGATACTTCC CCACTGTGCC AGGTGTGTCG CACACGGGGG TCTTGGCGGA 
TGTCAGCAGT CAGGAAGGGG CAGCACTTGG AAGCAGAACC CAGAATGCTC AAGGAGGCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RhoD is a Golgi component with a role in anterograde protein transport from the ER to the plasma membrane.
Experimental cell research333(2)208-19(2015 May)
Blom M,Reis K,Nehru V,Blom H,Gad AK,Aspenstr?m P

Atypical Rho GTPases RhoD and Rif integrate cytoskeletal dynamics and membrane trafficking.
Biological chemistry395(5)477-84(2014 May)
Aspenstr?m P

RhoD binds the Rab5 effector Rabankyrin-5 and has a role in trafficking of the platelet-derived growth factor receptor.
Traffic (Copenhagen, Denmark)14(12)1242-54(2013 Dec)
Nehru V,Voytyuk O,Lennartsson J,Aspenstr?m P

Rif proteins take to the RhoD: Rho GTPases at the crossroads of actin dynamics and membrane trafficking.
Cellular signalling22(2)183-9(2010 Feb)
Gad AK,Aspenstr?m P