Alx3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Alx3
Entrez Gene ID	11694
Full Name	aristaless-like homeobox 3
General protein information	Preferred Names aristaless-like homeobox 3 Names homeobox protein aristaless-like 3 proline-rich transcription factor Alx3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3 F2.3\|3 46.83 cM
Summary	This gene belongs to Group 1 of aristaless-like genes, which are characterized by the presence of an aristaless domain and a conserved paired-like homeodomain. The encoded protein acts as a transcriptional regulator. The protein plays a role in the development of craniofacial and appendicular skeleton and may have a role in pancreatic function. [provided by RefSeq, Apr 2013].

mRNA and Protein(s)

mRNA	Protein	Name
NM_007441.3	NP_031467.1	homeobox protein aristaless-like 3

Pathways from BioSystems

Homologies

	ALX3	XP_001099295.1
Canis lupus familiaris (dog)	ALX3	XP_005621859.1
Bos taurus (cattle)	ALX3	NP_001179482.1
Gallus gallus (chicken)	ALX3	XP_003642783.2
Homo sapiens (human)	ALX3	NP_006483.2
Pan troglodytes (chimpanzee)	ALX3	XP_524801.2
Mus musculus (house mouse)	Alx3	NP_031467.1
Rattus norvegicus (Norway rat)	Alx3	NP_001007013.1
Danio rerio (zebrafish)	LOC566955	XP_005167168.1

Gene Ontology
Bibliography

Related articles in PubMed

The aristaless-like homeobox protein Alx3 as an etiopathogenic factor for diabetes mellitus.
Vallejo M
Islets3(2)66-8(2011 Mar-Apr)

Alx3-deficient mice exhibit decreased insulin in beta cells, altered glucose homeostasis and increased apoptosis in pancreatic islets.
Mirasierra M, Fern?ndez-P?rez A, D?az-Prieto N, Vallejo M
Diabetologia54(2)403-14(2011 Feb)

Alx3-deficient mice exhibit folic acid-resistant craniofacial midline and neural tube closure defects.
Lakhwani S, Garc?a-Sanz P, Vallejo M
Developmental biology344(2)869-80(2010 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Glucose-stimulated expression of ALX3 in alpha cells provides a regulatory mechanism for the downregulation of glucagon gene expression by interfering with PAX6-mediated transactivation on the glucagon G1 promoter element.
Title: Glucose-dependent downregulation of glucagon gene expression mediated by selective interactions between ALX3 and PAX6 in mouse alpha cells.

Pdx1, USF1 and USF2 co-ordinately regulate Alx3 gene expression in pancreatic beta-cells.
Title: Pdx1 and USF transcription factors co-ordinately regulate Alx3 gene expression in pancreatic ?-cells.

Alx3 promoter activity in mesenchymal and pancreatic cells is regulatd by USF transcription factors and Twist1
Title: Differential configurations involving binding of USF transcription factors and Twist1 regulate Alx3 promoter activity in mesenchymal and pancreatic cells.

results indicate that Alx3 expression is enhanced by BMP-2 via the BMP receptors mediated-Smad signaling and that Alx3 is a positive regulator of osteoblast differentiation induced by BMP-2
Title: BMP-2 Induced Expression of Alx3 That Is a Positive Regulator of Osteoblast Differentiation.

ALX3 could be considered as a candidate gene for the etiopathogenesis of diabetes or its complications during embryonic or fetal development is discussed
Title: The aristaless-like homeobox protein Alx3 as an etiopathogenic factor for diabetes mellitus.

The following Alx3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Alx3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu19754	NM_007441.3 Latest version!	Mus musculus aristaless-like homeobox 3 (Alx3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Alx3 ( NM_007441.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031467.1 Mus musculus aristaless-like homeobox 3 (Alx3), mRNA.

Price & Availability↑

CloneID	OMu19754
Clone ID Related Accession (Same CDS sequence)	NM_007441.3
Accession Version	NM_007441.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-10
Organism	Mus musculus(house mouse)
Product	homeobox protein aristaless-like 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC122902.4 and AK142019.1. On Apr 16, 2013 this sequence version replaced NM_007441.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U96109.1, AK142019.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN01164131 [ECO:0000348] ##Evidence-Data-END##

ATGGACCCCG AGCGCTGCGC GCCTTTCTCC GTGGGGCCGG CGGCCGGTCC TTACGCGGCC 
GCGGGGGACG AGGCTCCGGG TCCCCAGGGG ACTCCCGATG CTGCGCCTCA CCTGCACCCC 
GCGCCACCCC GCGGTCCGCG CCTGAGCCGC TTCCCGGCCT GTGGGCCCCT GGAACCCTAC 
CTCCCAGAGC CCGCCAAGCC GCCCGCCAAG TACCTGCAGG ACCTCGGGCC AGGCCCGGTG 
CTCAACGGCG GCCACTTCTA CGAGGGCTCC GCGGAAGCCG AGGAGAAGGC CTCCAAAGCT 
GCCAGCTTCC CCCAGCTACC AGTGGATTGC CGAGGGGGTC CCAGGGACGG ACCCTCTAAT 
GTGCAAGCTT CCCCGGGCCC CTGCCTGGCC AGCCTGAGCG TCCCTCTTTC CCCGGGACTC 
CCCGACTCCA TGGAGTTGGC CAAGACCAAG AGCAAGAAGC GCCGGAACCG CACAACCTTC 
AGCACGTTCC AGCTGGAAGA GCTGGAGAAA GTCTTCCAGA AAACCCACTA CCCTGACGTG 
TATGCTCGGG AGCAGCTGGC TTTGCGAACA GACCTGACTG AGGCCAGGGT ACAGGTCTGG 
TTCCAGAACC GAAGAGCCAA GTGGCGGAAG CGTGAGCGTT ATGGGAAGAT GCAGGAGGGG 
CGGAACCCCT TCACTACAGC CTATGACATC TCCGTACTGC CCAGAACTGA CAGCCATCCT 
CAGCTGCAGA ACTCCCTGTG GCCCAGTCCA GGATCTGGAA GCCCAGGGGG GCCCTGTCTC 
ATGTCTCCAG AGGGCATCCC CTCTCCATGC ATGTCCCCAT ACTCCCACTC CCATGGAAAT 
GTGGCTGGCT TCATGGGAGT GCCAGCCTCC CCTGCAGCCC ACCCTGGCAT CTATTCCATC 
CATGGCTTCC CTCCTGCCCT GGGAGGGCAC AGCTTTGAGC CTTCTCCGGA TGGTGACTAC 
AAGTCCCCAA GCCTCGTTTC ACTCAGGATG AAGCCCAAAG AGCCCCCTGG CCTGCTGAAC 
TGGACCACGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031467.1
CDS	146..1177
Translation	MDPERCAPFSVGPAAGPYAAAGDEAPGPQGTPDAAPHLHPAPPRGPRLSRFPACGPLEPYLPEPAKPPAKYLQDLGPGPVLNGGHFYEGSAEAEEKASKAASFPQLPVDCRGGPRDGPSNVQASPGPCLASLSVPLSPGLPDSMELAKTKSKKRRNRTTFSTFQLEELEKVFQKTHYPDVYAREQLALRTDLTEARVQVWFQNRRAKWRKRERYGKMQEGRNPFTTAYDISVLPRTDSHPQLQNSLWPSPGSGSPGGPCLMSPEGIPSPCMSPYSHSHGNVAGFMGVPASPAAHPGIYSIHGFPPALGGHSFEPSPDGDYKSPSLVSLRMKPKEPPGLLNWTT

Target ORF information:

RefSeq Version	NM_007441.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus aristaless-like homeobox 3 (Alx3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007441.3

ORF Insert Sequence:

ATGGACCCCG AGCGCTGCGC GCCTTTCTCC GTGGGGCCGG CGGCCGGTCC TTACGCGGCC 
GCGGGGGACG AGGCTCCGGG TCCCCAGGGG ACTCCCGATG CTGCGCCTCA CCTGCACCCC 
GCGCCACCCC GCGGTCCGCG CCTGAGCCGC TTCCCGGCCT GTGGGCCCCT GGAACCCTAC 
CTCCCAGAGC CCGCCAAGCC GCCCGCCAAG TACCTGCAGG ACCTCGGGCC AGGCCCGGTG 
CTCAACGGCG GCCACTTCTA CGAGGGCTCC GCGGAAGCCG AGGAGAAGGC CTCCAAAGCT 
GCCAGCTTCC CCCAGCTACC AGTGGATTGC CGAGGGGGTC CCAGGGACGG ACCCTCTAAT 
GTGCAAGCTT CCCCGGGCCC CTGCCTGGCC AGCCTGAGCG TCCCTCTTTC CCCGGGACTC 
CCCGACTCCA TGGAGTTGGC CAAGACCAAG AGCAAGAAGC GCCGGAACCG CACAACCTTC 
AGCACGTTCC AGCTGGAAGA GCTGGAGAAA GTCTTCCAGA AAACCCACTA CCCTGACGTG 
TATGCTCGGG AGCAGCTGGC TTTGCGAACA GACCTGACTG AGGCCAGGGT ACAGGTCTGG 
TTCCAGAACC GAAGAGCCAA GTGGCGGAAG CGTGAGCGTT ATGGGAAGAT GCAGGAGGGG 
CGGAACCCCT TCACTACAGC CTATGACATC TCCGTACTGC CCAGAACTGA CAGCCATCCT 
CAGCTGCAGA ACTCCCTGTG GCCCAGTCCA GGATCTGGAA GCCCAGGGGG GCCCTGTCTC 
ATGTCTCCAG AGGGCATCCC CTCTCCATGC ATGTCCCCAT ACTCCCACTC CCATGGAAAT 
GTGGCTGGCT TCATGGGAGT GCCAGCCTCC CCTGCAGCCC ACCCTGGCAT CTATTCCATC 
CATGGCTTCC CTCCTGCCCT GGGAGGGCAC AGCTTTGAGC CTTCTCCGGA TGGTGACTAC 
AAGTCCCCAA GCCTCGTTTC ACTCAGGATG AAGCCCAAAG AGCCCCCTGG CCTGCTGAAC 
TGGACCACGT GA