Arhgef28 gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_006517521.3	XP_006517584.1	rho guanine nucleotide exchange factor 28 isoform X5
XM_006517521.4	XP_006517584.1	rho guanine nucleotide exchange factor 28 isoform X6
XM_017315361.1	XP_017170850.1	rho guanine nucleotide exchange factor 28 isoform X2
XM_030247128.1	XP_030102988.1	rho guanine nucleotide exchange factor 28 isoform X3
XM_006517518.2	XP_006517581.1	rho guanine nucleotide exchange factor 28 isoform X1
XM_006517519.4	XP_006517582.1	rho guanine nucleotide exchange factor 28 isoform X4
XM_006517519.3	XP_006517582.1	rho guanine nucleotide exchange factor 28 isoform X3
XM_017315362.1	XP_017170851.1	rho guanine nucleotide exchange factor 28 isoform X4
XM_006517518.3	XP_006517581.1	rho guanine nucleotide exchange factor 28 isoform X1
XM_017315361.2	XP_017170850.1	rho guanine nucleotide exchange factor 28 isoform X2
XM_017315362.2	XP_017170851.1	rho guanine nucleotide exchange factor 28 isoform X5
XM_006517522.3	XP_006517585.1	rho guanine nucleotide exchange factor 28 isoform X7
NM_012026.2	NP_036156.2	rho guanine nucleotide exchange factor 28

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu48947	XM_006517519.4 Latest version!	Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu48949	XM_006517521.4 Latest version!	Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu78410	XM_017315362.2 Latest version!	Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu86213	XM_030247128.1 Latest version!	Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu48950	XM_006517522.3 Latest version!	Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu48946	XM_006517518.3 Latest version!	Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu78409	XM_017315361.2 Latest version!	Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu09358	NM_012026.2 Latest version!	Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
View Old Accession Versions >>
OMu48949	XM_006517521.3	Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu48947	XM_006517519.3	Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu48946	XM_006517518.2	Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu78409	XM_017315361.1	Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu78410	XM_017315362.1	Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
Hide Old Accession Versions >>

Arhgef28 ( XM_006517519.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517582.1 Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu48947
Clone ID Related Accession (Same CDS sequence)	XM_006517519.4 , XM_006517519.3
Accession Version	XM_006517519.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4164bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rho guanine nucleotide exchange factor 28 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006517519.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCTG ACTCGGACTC ACCTTTCAAC TACTCCTGGC CTTCCTTCCC CAAGATGAAG 
ATTCGGCGCA GGGCATCCAA GAAAGATCGC TCCTTTGATG GCCTCAAAAA ATCCAAGCAT 
GTCCCAGCGT CCCTTGCTGC AGGCCAGCTC TCTGATGTTC TGAACGGGGG TGATGAAGTC 
TACGCTAACT GTATGGTGAT TGACCAGGTT GGCGATTTGG ATATCAACTA TATAAACCTA 
GAGGGACTCT CCACCCATAC GAGTCCCGAG TCTGGGAGGT CCATGCTGGG GCCTCAGGCT 
TGCATGCACA CCCTTCCTCC CGACACCAGT CCCTGCGGGC GCCCACTCAT TGAGAACAGT 
GAGGGGACCC TGGATGCTGC AGCTTCGCAG TCTTTCGTGA CACCATCCAG TTCTCGCACT 
TCCAACTTGA ACCTTTCTTT TGGTCTCCAT GGATTTGAAA AGGAGCAGAG TCATCTCAAG 
AAAAGGAGTT CTAGCCTGGA TGCCCTGGTT GCCGACAGTG AGGGGGAAGG AGGTTCTGAG 
CCCCCCATCT GCTATGCTGT GGGCTCTCAG AGTTCCCCAA GAACTGGACT CCCCAGCGGG 
GATGAACTGG ACTCTTTTGA GACCAACACT GAACCAGACT GTAATATCTC CAGGACCGAA 
TCACTCTCTC TGTCCAGTAC TCTGCATTCA AAGGAGTCCT TGCTTTCTGG GATCCGGTCT 
CGCTCCTACT CCTGCTCATC ACCCAAAATC TCTTCAGGAA AATCCCGGCT AGTGCGCGAC 
TTCACAGTGT GCAGTACCAG CGAAGAACAG AGATCATATA GTTTCCAGGA GCCACCAGGA 
GAAAAAAGGA TTCAGGAAGA AGAATGGGAT GAATACGTCA TTCCTGCCAA ATCCGAGTCT 
GAAAAGTACA AAGTGAGCCG AACTTTCAGC TTCCTCATGA ACAGGATGAC CAGCCCACGG 
AACAAATCTA AGATGAAAAA CAAGGACACT AAAGAAAAAG AAAAAATGAA TCGACATCAG 
TTTGTCCCGG GAACGTTCTC CGGGGTTCTG CAGTGCTCCG GTTGTGATAA AACACTCCTG 
GGGAAAGAGT CACTCCAGTG TGCCAACTGT AAGGCAAACA CACACAAGGG CTGCAAAGAT 
GCTGTGCCTC CGTGTACCAA GAAATTCCAA GAGAAATACA ACAAGAACAA ACCACAGAGC 
ATCCTTGGAA GCTCTTCAGT CAGAGACGTC CCAGCACCTG GGCTCTCCTT GCACCCTTCT 
TCCTCTATGC CCATCGGACT GCCAGCTGGA AGGAAGGAAT TTGCGGCTCA GGTGCATCCA 
CTGTCCAGAA GTGTCCCCGG CACCACTTTG GAAAGCTTCA GGAGGGCAGT GACATCGTTA 
GAGTCTGAGG GTGACAGCTG GAGAAGCAGG TCTCATTCCG ATGAGCTGTT CCAGTCTATG 
GGCTCTTCTC CTTCCACGGA GTCATTCATG ATGGAAGATG TTGTGGATTC TTCTCTGTGG 
ATCGACCTCA GTAGTGATGC TCAGGAGTTT GAAGCAGAGT CTTGGAGTCT CGTGGTGGAC 
CCTTCCTTTT GCAGTAGGCA GGAGAAGGAT GTCATCAAAA GACAAGATGT CATTTTTGAG 
CTGATGCAGA CAGAGGTCCA CCACATCCAG ACTCTGCTCA TCATGTCCGA GGTCTTCAGG 
AAAGGCATGA AGGAGGAACT GCAGCTGGAC CACAGCACGG TGGACAAGAT CTTCCCCTGC 
TTAGATGAGC TGCTGGAGAC TCACCGGCAC TTCTTCTTCA GCATGAAGGA GCGCAGGCAG 
GAGTCCTGCG CTGGCAGTGA CAGGAACTTT GTGATCAACC AAATTGGAGA CATTCTGGTA 
CAACAGTTTT CAGAGGAAAA TGCAAGCAAA ATGAAGAGGA TCTATGGAGA ATTCTGCAGC 
CATCACAAAG AAGCCATGAG TCTCTTTAAA GAGCTGCAGC AGAACAAGAA GTTTCAGAAT 
TTTATTAAGA TCCGAAATAG CAACCTGTTG GCTCGTCGCC GCGGAATCCC GGAGTGCATC 
CTGCTAGTTA CCCAGCGCAT CACAAAGTAC CCAGTCTTGG TGGAAAGGAT CCTGCAGTAC 
ACCAAGGAAA GGACTGAAGA GCACCGGGAC CTGTGCAAAG CTCTCGGCTT AATTAAAGAC 
ATGATCGCAG CCGTGGACCT GAAAGTCAGC GAATACGAGA AGAACCAAAA ATGGCTCGAG 
ATCCTAAATA AAATCGAGAA CAAAACATAC ACAAAGCTCA AAAATGGCCA CGTGTTTAGA 
AAGCAGGCGC TGCTGAGCCA GGAACGGGCT CTCCTGCATG ATGGGCTTGT CTACTGGAAA 
ACCGCTACGG GTCGCTTCAA AGACATCCTG GCTCTGCTAC TAACTGACGT GCTGCTCTTC 
TTACAAGAAA AAGACCAGAA ATACATCTTT GCAGCTGTTG ACCAGAAGCC ATCAGTGATT 
TCCCTCCAGA AGCTGATTGC TAGAGAAGTT GCCAACGAGG AGAGAGGCAT GTTTCTGATC 
AGTGCTTCGT CAGCCGGCCC CGAGATGTAC GAAATCCACA CCAATTCCAA GGAGGAGCGG 
AATAACTGGA TGAGGCGGAT CCAGCAGGCA GTAGAAAGTT GTCCAGAGGA AGAAGGAGGA 
AGGACGAGCG AGTCGGATGA AGAGAGGCGG AAAGCTGAAG CTAGAGTGGC CAAGATTCAG 
CAATGTCAAG AAATACTCAG TAACCAAGAC CAACAGATTT GCACGTACTT GGAAGAAAAG 
CTGCATATCT ACGCTGAACT TGGGGAACTG AGTGGCTTTG AGGATGTCCA CCTAGAACCC 
CATCTGCTCA TCAAACCTGA CCCTGGAGAG CCTCCCCAGG CAGCGTCCCT GCTTGCAGCA 
GCCCTGAGAG AAGCCGAGAG TCTGCAAGTG GCTGTGAAGG CTTCAAAGAT GGGCGATGTA 
AGCCAGTCAT CCGAAGAAAG CCCTGGAGGG ACTGTCCTGA TGGACACACC CAGTACACAG 
GATGTACCAG CGTCACCAAC TGCTTCATTA GTCACAGAAG GGACAGAAGG AAGAGGCTGT 
TGGGATGTGG ATCCCGGGCT CCAGGGTGTG GTAACCGACT TGGCAGTCTC TGATGCAGGG 
GAGAAGGTGG AATATAGAAG TTTCTCCGGT TCTTCACAGT CAGAGATTAT ACAAGCAATA 
CAGAATTTAA CGCGTCTCCT CTACAGCCTT CAGGCTGCCT TGACCATCCA GGACAGTCAC 
ATTGAGATCC ACAAACTGGT TCTACAGCAG CGGGAGAGCT TGGCCCCTAG CCACTCCTTC 
CGAGGTGGCC CCTTGCAAGA CCAGGAGAAA TCCCGCTACC TGGAAAAGCA GCGTGAAGAG 
CTGGCCAACA TCCACAAGCT TCAACACCAG TTCCAGCAGG AGCAGCGGCG CTGGCACCGC 
ACGTGCGACC AGCAACAGCG CGAGCAGGAA GCCCAAGAGA GCTGGCTGCA GGCGCGGGAA 
CGTGAGTGCC AGTCCCAGGA GGAATTGTTG CTGCGCCACC GTAGCGAGCT GGACCACCAA 
CTCCAGGAGT ACCAGCAGAG CCTTGAGCGG CTACGGGAGG GCCAGCGGAT GGTGGAGAGG 
GAGAGACAGA AGATGCGTGT CCAGCAGGGT CTGCTGGGCC ACTGCAAGCA TAGTCGCCAG 
CGGAGCCTCC CAGCCGTCTT CTCTCCAGGG AGCAAGGAGG TAACAGAACT TAACCGAGCT 
GAGAGTTTGT GTCATGAAAA TTCATTTTTC ATCAATGAAG CTTTTGGACA CATGTCACTT 
AACACGTCCA ATAAACCAAA CCCATCAGGG GTCCCCTGGG ATGCTCACCC CCTGGAGGGG 
TCTCATTTTG ATTTGGCAAG GACTAGTGAG AGTCCAACAG AACTGAAGAT AGACATTTCT 
CAGCCTCCTG ATGTCAACTC TGAACTCTGG ACAACGGGGC CGGGCCATCA GAGACCTGCT 
CTCCAAGAAA ACAGCAAGGA GTCTTATAAA AATGTTGCAG ATTTGGACTC CTTCCAGAGT 
GAATCGTCAT CTCCCCAGGA CTCCAATCAG CGTGGCCCCC AGCCACAGAC ACTCACAACA 
GAAGCAAAAC TAAGTCTTCC GATGGCGGCT GGACACGGTG GCGATGCTGG AGACGGGGCT 
GAAGAAAACA TTCTTTACCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517582.1
CDS	1823..5986
Translation	MDSDSDSPFNYSWPSFPKMKIRRRASKKDRSFDGLKKSKHVPASLAAGQLSDVLNGGDEVYANCMVIDQVGDLDINYINLEGLSTHTSPESGRSMLGPQACMHTLPPDTSPCGRPLIENSEGTLDAAASQSFVTPSSSRTSNLNLSFGLHGFEKEQSHLKKRSSSLDALVADSEGEGGSEPPICYAVGSQSSPRTGLPSGDELDSFETNTEPDCNISRTESLSLSSTLHSKESLLSGIRSRSYSCSSPKISSGKSRLVRDFTVCSTSEEQRSYSFQEPPGEKRIQEEEWDEYVIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKMKNKDTKEKEKMNRHQFVPGTFSGVLQCSGCDKTLLGKESLQCANCKANTHKGCKDAVPPCTKKFQEKYNKNKPQSILGSSSVRDVPAPGLSLHPSSSMPIGLPAGRKEFAAQVHPLSRSVPGTTLESFRRAVTSLESEGDSWRSRSHSDELFQSMGSSPSTESFMMEDVVDSSLWIDLSSDAQEFEAESWSLVVDPSFCSRQEKDVIKRQDVIFELMQTEVHHIQTLLIMSEVFRKGMKEELQLDHSTVDKIFPCLDELLETHRHFFFSMKERRQESCAGSDRNFVINQIGDILVQQFSEENASKMKRIYGEFCSHHKEAMSLFKELQQNKKFQNFIKIRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKERTEEHRDLCKALGLIKDMIAAVDLKVSEYEKNQKWLEILNKIENKTYTKLKNGHVFRKQALLSQERALLHDGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNNWMRRIQQAVESCPEEEGGRTSESDEERRKAEARVAKIQQCQEILSNQDQQICTYLEEKLHIYAELGELSGFEDVHLEPHLLIKPDPGEPPQAASLLAAALREAESLQVAVKASKMGDVSQSSEESPGGTVLMDTPSTQDVPASPTASLVTEGTEGRGCWDVDPGLQGVVTDLAVSDAGEKVEYRSFSGSSQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIHKLVLQQRESLAPSHSFRGGPLQDQEKSRYLEKQREELANIHKLQHQFQQEQRRWHRTCDQQQREQEAQESWLQARERECQSQEELLLRHRSELDHQLQEYQQSLERLREGQRMVERERQKMRVQQGLLGHCKHSRQRSLPAVFSPGSKEVTELNRAESLCHENSFFINEAFGHMSLNTSNKPNPSGVPWDAHPLEGSHFDLARTSESPTELKIDISQPPDVNSELWTTGPGHQRPALQENSKESYKNVADLDSFQSESSSPQDSNQRGPQPQTLTTEAKLSLPMAAGHGGDAGDGAEENILYL

Target ORF information:

RefSeq Version	XM_006517519.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006517519.4

ORF Insert Sequence:

ATGGATTCTG ACTCGGACTC ACCTTTCAAC TACTCCTGGC CTTCCTTCCC CAAGATGAAG 
ATTCGGCGCA GGGCATCCAA GAAAGATCGC TCCTTTGATG GCCTCAAAAA ATCCAAGCAT 
GTCCCAGCGT CCCTTGCTGC AGGCCAGCTC TCTGATGTTC TGAACGGGGG TGATGAAGTC 
TACGCTAACT GTATGGTGAT TGACCAGGTT GGCGATTTGG ATATCAACTA TATAAACCTA 
GAGGGACTCT CCACCCATAC GAGTCCCGAG TCTGGGAGGT CCATGCTGGG GCCTCAGGCT 
TGCATGCACA CCCTTCCTCC CGACACCAGT CCCTGCGGGC GCCCACTCAT TGAGAACAGT 
GAGGGGACCC TGGATGCTGC AGCTTCGCAG TCTTTCGTGA CACCATCCAG TTCTCGCACT 
TCCAACTTGA ACCTTTCTTT TGGTCTCCAT GGATTTGAAA AGGAGCAGAG TCATCTCAAG 
AAAAGGAGTT CTAGCCTGGA TGCCCTGGTT GCCGACAGTG AGGGGGAAGG AGGTTCTGAG 
CCCCCCATCT GCTATGCTGT GGGCTCTCAG AGTTCCCCAA GAACTGGACT CCCCAGCGGG 
GATGAACTGG ACTCTTTTGA GACCAACACT GAACCAGACT GTAATATCTC CAGGACCGAA 
TCACTCTCTC TGTCCAGTAC TCTGCATTCA AAGGAGTCCT TGCTTTCTGG GATCCGGTCT 
CGCTCCTACT CCTGCTCATC ACCCAAAATC TCTTCAGGAA AATCCCGGCT AGTGCGCGAC 
TTCACAGTGT GCAGTACCAG CGAAGAACAG AGATCATATA GTTTCCAGGA GCCACCAGGA 
GAAAAAAGGA TTCAGGAAGA AGAATGGGAT GAATACGTCA TTCCTGCCAA ATCCGAGTCT 
GAAAAGTACA AAGTGAGCCG AACTTTCAGC TTCCTCATGA ACAGGATGAC CAGCCCACGG 
AACAAATCTA AGATGAAAAA CAAGGACACT AAAGAAAAAG AAAAAATGAA TCGACATCAG 
TTTGTCCCGG GAACGTTCTC CGGGGTTCTG CAGTGCTCCG GTTGTGATAA AACACTCCTG 
GGGAAAGAGT CACTCCAGTG TGCCAACTGT AAGGCAAACA CACACAAGGG CTGCAAAGAT 
GCTGTGCCTC CGTGTACCAA GAAATTCCAA GAGAAATACA ACAAGAACAA ACCACAGAGC 
ATCCTTGGAA GCTCTTCAGT CAGAGACGTC CCAGCACCTG GGCTCTCCTT GCACCCTTCT 
TCCTCTATGC CCATCGGACT GCCAGCTGGA AGGAAGGAAT TTGCGGCTCA GGTGCATCCA 
CTGTCCAGAA GTGTCCCCGG CACCACTTTG GAAAGCTTCA GGAGGGCAGT GACATCGTTA 
GAGTCTGAGG GTGACAGCTG GAGAAGCAGG TCTCATTCCG ATGAGCTGTT CCAGTCTATG 
GGCTCTTCTC CTTCCACGGA GTCATTCATG ATGGAAGATG TTGTGGATTC TTCTCTGTGG 
ATCGACCTCA GTAGTGATGC TCAGGAGTTT GAAGCAGAGT CTTGGAGTCT CGTGGTGGAC 
CCTTCCTTTT GCAGTAGGCA GGAGAAGGAT GTCATCAAAA GACAAGATGT CATTTTTGAG 
CTGATGCAGA CAGAGGTCCA CCACATCCAG ACTCTGCTCA TCATGTCCGA GGTCTTCAGG 
AAAGGCATGA AGGAGGAACT GCAGCTGGAC CACAGCACGG TGGACAAGAT CTTCCCCTGC 
TTAGATGAGC TGCTGGAGAC TCACCGGCAC TTCTTCTTCA GCATGAAGGA GCGCAGGCAG 
GAGTCCTGCG CTGGCAGTGA CAGGAACTTT GTGATCAACC AAATTGGAGA CATTCTGGTA 
CAACAGTTTT CAGAGGAAAA TGCAAGCAAA ATGAAGAGGA TCTATGGAGA ATTCTGCAGC 
CATCACAAAG AAGCCATGAG TCTCTTTAAA GAGCTGCAGC AGAACAAGAA GTTTCAGAAT 
TTTATTAAGA TCCGAAATAG CAACCTGTTG GCTCGTCGCC GCGGAATCCC GGAGTGCATC 
CTGCTAGTTA CCCAGCGCAT CACAAAGTAC CCAGTCTTGG TGGAAAGGAT CCTGCAGTAC 
ACCAAGGAAA GGACTGAAGA GCACCGGGAC CTGTGCAAAG CTCTCGGCTT AATTAAAGAC 
ATGATCGCAG CCGTGGACCT GAAAGTCAGC GAATACGAGA AGAACCAAAA ATGGCTCGAG 
ATCCTAAATA AAATCGAGAA CAAAACATAC ACAAAGCTCA AAAATGGCCA CGTGTTTAGA 
AAGCAGGCGC TGCTGAGCCA GGAACGGGCT CTCCTGCATG ATGGGCTTGT CTACTGGAAA 
ACCGCTACGG GTCGCTTCAA AGACATCCTG GCTCTGCTAC TAACTGACGT GCTGCTCTTC 
TTACAAGAAA AAGACCAGAA ATACATCTTT GCAGCTGTTG ACCAGAAGCC ATCAGTGATT 
TCCCTCCAGA AGCTGATTGC TAGAGAAGTT GCCAACGAGG AGAGAGGCAT GTTTCTGATC 
AGTGCTTCGT CAGCCGGCCC CGAGATGTAC GAAATCCACA CCAATTCCAA GGAGGAGCGG 
AATAACTGGA TGAGGCGGAT CCAGCAGGCA GTAGAAAGTT GTCCAGAGGA AGAAGGAGGA 
AGGACGAGCG AGTCGGATGA AGAGAGGCGG AAAGCTGAAG CTAGAGTGGC CAAGATTCAG 
CAATGTCAAG AAATACTCAG TAACCAAGAC CAACAGATTT GCACGTACTT GGAAGAAAAG 
CTGCATATCT ACGCTGAACT TGGGGAACTG AGTGGCTTTG AGGATGTCCA CCTAGAACCC 
CATCTGCTCA TCAAACCTGA CCCTGGAGAG CCTCCCCAGG CAGCGTCCCT GCTTGCAGCA 
GCCCTGAGAG AAGCCGAGAG TCTGCAAGTG GCTGTGAAGG CTTCAAAGAT GGGCGATGTA 
AGCCAGTCAT CCGAAGAAAG CCCTGGAGGG ACTGTCCTGA TGGACACACC CAGTACACAG 
GATGTACCAG CGTCACCAAC TGCTTCATTA GTCACAGAAG GGACAGAAGG AAGAGGCTGT 
TGGGATGTGG ATCCCGGGCT CCAGGGTGTG GTAACCGACT TGGCAGTCTC TGATGCAGGG 
GAGAAGGTGG AATATAGAAG TTTCTCCGGT TCTTCACAGT CAGAGATTAT ACAAGCAATA 
CAGAATTTAA CGCGTCTCCT CTACAGCCTT CAGGCTGCCT TGACCATCCA GGACAGTCAC 
ATTGAGATCC ACAAACTGGT TCTACAGCAG CGGGAGAGCT TGGCCCCTAG CCACTCCTTC 
CGAGGTGGCC CCTTGCAAGA CCAGGAGAAA TCCCGCTACC TGGAAAAGCA GCGTGAAGAG 
CTGGCCAACA TCCACAAGCT TCAACACCAG TTCCAGCAGG AGCAGCGGCG CTGGCACCGC 
ACGTGCGACC AGCAACAGCG CGAGCAGGAA GCCCAAGAGA GCTGGCTGCA GGCGCGGGAA 
CGTGAGTGCC AGTCCCAGGA GGAATTGTTG CTGCGCCACC GTAGCGAGCT GGACCACCAA 
CTCCAGGAGT ACCAGCAGAG CCTTGAGCGG CTACGGGAGG GCCAGCGGAT GGTGGAGAGG 
GAGAGACAGA AGATGCGTGT CCAGCAGGGT CTGCTGGGCC ACTGCAAGCA TAGTCGCCAG 
CGGAGCCTCC CAGCCGTCTT CTCTCCAGGG AGCAAGGAGG TAACAGAACT TAACCGAGCT 
GAGAGTTTGT GTCATGAAAA TTCATTTTTC ATCAATGAAG CTTTTGGACA CATGTCACTT 
AACACGTCCA ATAAACCAAA CCCATCAGGG GTCCCCTGGG ATGCTCACCC CCTGGAGGGG 
TCTCATTTTG ATTTGGCAAG GACTAGTGAG AGTCCAACAG AACTGAAGAT AGACATTTCT 
CAGCCTCCTG ATGTCAACTC TGAACTCTGG ACAACGGGGC CGGGCCATCA GAGACCTGCT 
CTCCAAGAAA ACAGCAAGGA GTCTTATAAA AATGTTGCAG ATTTGGACTC CTTCCAGAGT 
GAATCGTCAT CTCCCCAGGA CTCCAATCAG CGTGGCCCCC AGCCACAGAC ACTCACAACA 
GAAGCAAAAC TAAGTCTTCC GATGGCGGCT GGACACGGTG GCGATGCTGG AGACGGGGCT 
GAAGAAAACA TTCTTTACCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgef28 ( XM_006517521.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517584.1 Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OMu48949
Clone ID Related Accession (Same CDS sequence)	XM_006517521.3 , XM_006517521.4
Accession Version	XM_006517521.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rho guanine nucleotide exchange factor 28 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006517521.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCGGC GACGAGCCCG GCTGGCCAGA GATGCAGGAG CCATGGAGTT GAGCTGCAGT 
GAAGTGCCTC TTTATGGACA GAAGACGGTC TATGCAAAGT TTGGCAAAAA CGTGTATCTA 
CCCGAAGATG CGGAGTTTTA CTTTGTTTAT GGCGGGTCGC ATCAGAGGCA CGTGGTGATT 
GCTGACCGTG TCCAGGACAA TGTCCTCCAA TCTAGCATTC CAGGCCACTG GCTTCAGGAG 
ACGGTGACGG TATCTGTGTG CCTCTGCTCG GAAGGGTACT CCCCAGTGAC CATGGGCTCT 
GGCTCAGTGA CCTACGTGGA CAACATGGCC TGCAGGCTGG CTCGTCTGCT GGTCACTCAG 
GCTGACCGAC TCACGGCCTG CAGTCACCAG ACGCTGCTGA CCCCGTTTGC CCTGACAGTG 
GAAGCGCTGC CTGCCTTGGA TGAGGAGCTC GTGCTGGCTC TGACCCAACT GGAGCTGCCT 
TTGGGTTGGA CAGTCCTGGG AAATTCCTCA CTTGAAGTAT CTCTACACAG AGAGTCTCTC 
CTGCATCTGG CTGTGAGATG GGCCCTCCCT AAGCTATTCC ACTTCCTCTT GTGTCTCCCT 
GGAGGAGTCA AGGCCTTGAA GCTACCTAAT GAAGAGGCCA CCACACCATT AGACTTAGCT 
TTGCAAGGTG GACACTCCAC ACTGGTAGAA GACATCACAA ATTTTCAGGG TAGCCATTCC 
CCAGGCTTCT CCCGACTGCG TCTTAACGAA GAGGCCACTC TGCAGTTTGT TCATTCATCG 
GAAACATTGA CCCTGACCGT TAACCACACA GCTGAGCATC TCTTGGAAGC AGACATCAAA 
CTCTTCCGGA AATATTTCTG GGACAGAGCC TTTCTTGTCA AGGCTCTTGA GCAAGAAGCC 
AAGACTGAGA AAGCAACCAT GCCTTCTGGA GCTGCAGAAA CTGAAGAAGA GGTTAGGAAT 
TTGGAGTCTG GCAGATCACC ATCGGAGGAG GAGGAGGATG CAAAGAGCAT CAAAAGCCAG 
GTGGATGGGC CGAGTGAACA CGAAGACCAG GACCGCCTAC CTCTGGATCG CTCCTTTGAT 
GGCCTCAAAA AATCCAAGCA TGTCCCAGCG TCCCTTGCTG CAGGCCAGCT CTCTGATGTT 
CTGAACGGGG GTGATGAAGT CTACGCTAAC TGTATGGTGA TTGACCAGGT TGGCGATTTG 
GATATCAACT ATATAAACCT AGAGGGACTC TCCACCCATA CGAGTCCCGA GTCTGGGAGG 
TCCATGCTGG GGCCTCAGGC TTGCATGCAC ACCCTTCCTC CCGACACCAG TCCCTGCGGG 
CGCCCACTCA TTGAGAACAG TGAGGGGACC CTGGATGCTG CAGCTTCGCA GTCTTTCGTG 
ACACCATCCA GTTCTCGCAC TTCCAACTTG AACCTTTCTT TTGGTCTCCA TGGATTTGAA 
AAGGAGCAGA GTCATCTCAA GAAAAGGAGT TCTAGCCTGG ATGCCCTGGT TGCCGACAGT 
GAGGGGGAAG GAGGTTCTGA GCCCCCCATC TGCTATGCTG TGGGCTCTCA GAGTTCCCCA 
AGAACTGGAC TCCCCAGCGG GGATGAACTG GACTCTTTTG AGACCAACAC TGAACCAGAC 
TGTAATATCT CCAGGACCGA ATCACTCTCT CTGTCCAGTA CTCTGCATTC AAAGGAGTCC 
TTGCTTTCTG GGATCCGGTC TCGCTCCTAC TCCTGCTCAT CACCCAAAAT CTCTTCAGGA 
AAATCCCGGC TAGTGCGCGA CTTCACAGTG TGCAGTACCA GCGAAGAACA GAGATCATAT 
AGTTTCCAGG AGCCACCAGG AGAAAAAAGG ATTCAGGAAG AAGAATGGGA TGAATACGTC 
ATTCCTGCCA AATCCGAGTC TGAAAAGTAC AAAGTGAGCC GAACTTTCAG CTTCCTCATG 
AACAGGATGA CCAGCCCACG GAACAAATCT AAGATGAAAA ACAAGGACAC TAAAGAAAAA 
GAAAAAATGA ATCGACATCA GTTTGTCCCG GGAACGTTCT CCGGGGTTCT GCAGTGCTCC 
GGTTGTGATA AAACACTCCT GGGGAAAGAG TCACTCCAGT GTGCCAACTG TAAGGCAAAC 
ACACACAAGG GCTGCAAAGA TGCTGTGCCT CCGTGTACCA AGAAATTCCA AGAGAAATAC 
AACAAGAACA AACCACAGAG CATCCTTGGA AGCTCTTCAG TCAGAGACGT CCCAGCACCT 
GGGCTCTCCT TGCACCCTTC TTCCTCTATG CCCATCGGAC TGCCAGCTGG AAGGAAGGAA 
TTTGCGGCTC AGGTGCATCC ACTGTCCAGA AGTGTCCCCG GCACCACTTT GGAAAGCTTC 
AGGAGGGCAG TGACATCGTT AGAGTCTGAG GGTGACAGCT GGAGAAGCAG GTCTCATTCC 
GATGAGCTGT TCCAGTCTAT GGGCTCTTCT CCTTCCACGG AGTCATTCAT GATGGAAGAT 
GTTGTGGATT CTTCTCTGTG GATCGACCTC AGTAGTGATG CTCAGGAGTT TGAAGCAGAG 
TCTTGGAGTC TCGTGGTGGA CCCTTCCTTT TGCAGTAGGC AGGAGAAGGA TGTCATCAAA 
AGACAAGATG TCATTTTTGA GCTGATGCAG ACAGAGGTCC ACCACATCCA GACTCTGCTC 
ATCATGTCCG AGGTCTTCAG GAAAGGCATG AAGGAGGAAC TGCAGCTGGA CCACAGCACG 
GTGGACAAGA TCTTCCCCTG CTTAGATGAG CTGCTGGAGA CTCACCGGCA CTTCTTCTTC 
AGCATGAAGG AGCGCAGGCA GGAGTCCTGC GCTGGCAGTG ACAGGAACTT TGTGATCAAC 
CAAATTGGAG ACATTCTGGT ACAACAGTTT TCAGAGGAAA ATGCAAGCAA AATGAAGAGG 
ATCTATGGAG AATTCTGCAG CCATCACAAA GAAGCCATGA GTCTCTTTAA AGAGCTGCAG 
CAGAACAAGA AGTTTCAGAA TTTTATTAAG ATCCGAAATA GCAACCTGTT GGCTCGTCGC 
CGCGGAATCC CGGAGTGCAT CCTGCTAGTT ACCCAGCGCA TCACAAAGTA CCCAGTCTTG 
GTGGAAAGGA TCCTGCAGTA CACCAAGGAA AGGACTGAAG AGCACCGGGA CCTGTGCAAA 
GCTCTCGGCT TAATTAAAGA CATGATCGCA GCCGTGGACC TGAAAGTCAG CGAATACGAG 
AAGAACCAAA AATGGCTCGA GATCCTAAAT AAAATCGAGA ACAAAACATA CACAAAGCTC 
AAAAATGGCC ACGTGTTTAG AAAGCAGGCG CTGCTGAGCC AGGAACGGGC TCTCCTGCAT 
GATGGGCTTG TCTACTGGAA AACCGCTACG GGTCGCTTCA AAGACATCCT GGCTCTGCTA 
CTAACTGACG TGCTGCTCTT CTTACAAGAA AAAGACCAGA AATACATCTT TGCAGCTGTT 
GACCAGAAGC CATCAGTGAT TTCCCTCCAG AAGCTGATTG CTAGAGAAGT TGCCAACGAG 
GAGAGAGGCA TGTTTCTGAT CAGTGCTTCG TCAGCCGGCC CCGAGATGTA CGAAATCCAC 
ACCAATTCCA AGGAGGAGCG GAATAACTGG ATGAGGCGGA TCCAGCAGGC AGTAGAAAGT 
TGTCCAGAGG AAGAAGGAGG AAGGACGAGC GAGTCGGATG AAGAGAGGCG GAAAGCTGAA 
GCTAGAGTGG CCAAGATTCA GCAATGTCAA GAAATACTCA GTAACCAAGA CCAACAGATT 
TGCACGTACT TGGAAGAAAA GCTGCATATC TACGCTGAAC TTGGGGAACT GAGTGGCTTT 
GAGGATGTCC ACCTAGAACC CCATCTGCTC ATCAAACCTG ACCCTGGAGA GCCTCCCCAG 
GCAGCGTCCC TGCTTGCAGC AGCCCTGAGA GAAGCCGAGA GTCTGCAAGT GGCTGTGAAG 
GCTTCAAAGA TGGGCGATGT AAGCCAGTCA TCCGAAGAAA GCCCTGGAGG GACTGTCCTG 
ATGGACACAC CCAGTACACA GGATGTACCA GCGTCACCAA CTGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517584.1
CDS	322..4329
Translation	MRRRRARLARDAGAMELSCSEVPLYGQKTVYAKFGKNVYLPEDAEFYFVYGGSHQRHVVIADRVQDNVLQSSIPGHWLQETVTVSVCLCSEGYSPVTMGSGSVTYVDNMACRLARLLVTQADRLTACSHQTLLTPFALTVEALPALDEELVLALTQLELPLGWTVLGNSSLEVSLHRESLLHLAVRWALPKLFHFLLCLPGGVKALKLPNEEATTPLDLALQGGHSTLVEDITNFQGSHSPGFSRLRLNEEATLQFVHSSETLTLTVNHTAEHLLEADIKLFRKYFWDRAFLVKALEQEAKTEKATMPSGAAETEEEVRNLESGRSPSEEEEDAKSIKSQVDGPSEHEDQDRLPLDRSFDGLKKSKHVPASLAAGQLSDVLNGGDEVYANCMVIDQVGDLDINYINLEGLSTHTSPESGRSMLGPQACMHTLPPDTSPCGRPLIENSEGTLDAAASQSFVTPSSSRTSNLNLSFGLHGFEKEQSHLKKRSSSLDALVADSEGEGGSEPPICYAVGSQSSPRTGLPSGDELDSFETNTEPDCNISRTESLSLSSTLHSKESLLSGIRSRSYSCSSPKISSGKSRLVRDFTVCSTSEEQRSYSFQEPPGEKRIQEEEWDEYVIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKMKNKDTKEKEKMNRHQFVPGTFSGVLQCSGCDKTLLGKESLQCANCKANTHKGCKDAVPPCTKKFQEKYNKNKPQSILGSSSVRDVPAPGLSLHPSSSMPIGLPAGRKEFAAQVHPLSRSVPGTTLESFRRAVTSLESEGDSWRSRSHSDELFQSMGSSPSTESFMMEDVVDSSLWIDLSSDAQEFEAESWSLVVDPSFCSRQEKDVIKRQDVIFELMQTEVHHIQTLLIMSEVFRKGMKEELQLDHSTVDKIFPCLDELLETHRHFFFSMKERRQESCAGSDRNFVINQIGDILVQQFSEENASKMKRIYGEFCSHHKEAMSLFKELQQNKKFQNFIKIRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKERTEEHRDLCKALGLIKDMIAAVDLKVSEYEKNQKWLEILNKIENKTYTKLKNGHVFRKQALLSQERALLHDGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNNWMRRIQQAVESCPEEEGGRTSESDEERRKAEARVAKIQQCQEILSNQDQQICTYLEEKLHIYAELGELSGFEDVHLEPHLLIKPDPGEPPQAASLLAAALREAESLQVAVKASKMGDVSQSSEESPGGTVLMDTPSTQDVPASPTA

Target ORF information:

RefSeq Version	XM_006517521.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006517521.4

ORF Insert Sequence:

ATGAGGCGGC GACGAGCCCG GCTGGCCAGA GATGCAGGAG CCATGGAGTT GAGCTGCAGT 
GAAGTGCCTC TTTATGGACA GAAGACGGTC TATGCAAAGT TTGGCAAAAA CGTGTATCTA 
CCCGAAGATG CGGAGTTTTA CTTTGTTTAT GGCGGGTCGC ATCAGAGGCA CGTGGTGATT 
GCTGACCGTG TCCAGGACAA TGTCCTCCAA TCTAGCATTC CAGGCCACTG GCTTCAGGAG 
ACGGTGACGG TATCTGTGTG CCTCTGCTCG GAAGGGTACT CCCCAGTGAC CATGGGCTCT 
GGCTCAGTGA CCTACGTGGA CAACATGGCC TGCAGGCTGG CTCGTCTGCT GGTCACTCAG 
GCTGACCGAC TCACGGCCTG CAGTCACCAG ACGCTGCTGA CCCCGTTTGC CCTGACAGTG 
GAAGCGCTGC CTGCCTTGGA TGAGGAGCTC GTGCTGGCTC TGACCCAACT GGAGCTGCCT 
TTGGGTTGGA CAGTCCTGGG AAATTCCTCA CTTGAAGTAT CTCTACACAG AGAGTCTCTC 
CTGCATCTGG CTGTGAGATG GGCCCTCCCT AAGCTATTCC ACTTCCTCTT GTGTCTCCCT 
GGAGGAGTCA AGGCCTTGAA GCTACCTAAT GAAGAGGCCA CCACACCATT AGACTTAGCT 
TTGCAAGGTG GACACTCCAC ACTGGTAGAA GACATCACAA ATTTTCAGGG TAGCCATTCC 
CCAGGCTTCT CCCGACTGCG TCTTAACGAA GAGGCCACTC TGCAGTTTGT TCATTCATCG 
GAAACATTGA CCCTGACCGT TAACCACACA GCTGAGCATC TCTTGGAAGC AGACATCAAA 
CTCTTCCGGA AATATTTCTG GGACAGAGCC TTTCTTGTCA AGGCTCTTGA GCAAGAAGCC 
AAGACTGAGA AAGCAACCAT GCCTTCTGGA GCTGCAGAAA CTGAAGAAGA GGTTAGGAAT 
TTGGAGTCTG GCAGATCACC ATCGGAGGAG GAGGAGGATG CAAAGAGCAT CAAAAGCCAG 
GTGGATGGGC CGAGTGAACA CGAAGACCAG GACCGCCTAC CTCTGGATCG CTCCTTTGAT 
GGCCTCAAAA AATCCAAGCA TGTCCCAGCG TCCCTTGCTG CAGGCCAGCT CTCTGATGTT 
CTGAACGGGG GTGATGAAGT CTACGCTAAC TGTATGGTGA TTGACCAGGT TGGCGATTTG 
GATATCAACT ATATAAACCT AGAGGGACTC TCCACCCATA CGAGTCCCGA GTCTGGGAGG 
TCCATGCTGG GGCCTCAGGC TTGCATGCAC ACCCTTCCTC CCGACACCAG TCCCTGCGGG 
CGCCCACTCA TTGAGAACAG TGAGGGGACC CTGGATGCTG CAGCTTCGCA GTCTTTCGTG 
ACACCATCCA GTTCTCGCAC TTCCAACTTG AACCTTTCTT TTGGTCTCCA TGGATTTGAA 
AAGGAGCAGA GTCATCTCAA GAAAAGGAGT TCTAGCCTGG ATGCCCTGGT TGCCGACAGT 
GAGGGGGAAG GAGGTTCTGA GCCCCCCATC TGCTATGCTG TGGGCTCTCA GAGTTCCCCA 
AGAACTGGAC TCCCCAGCGG GGATGAACTG GACTCTTTTG AGACCAACAC TGAACCAGAC 
TGTAATATCT CCAGGACCGA ATCACTCTCT CTGTCCAGTA CTCTGCATTC AAAGGAGTCC 
TTGCTTTCTG GGATCCGGTC TCGCTCCTAC TCCTGCTCAT CACCCAAAAT CTCTTCAGGA 
AAATCCCGGC TAGTGCGCGA CTTCACAGTG TGCAGTACCA GCGAAGAACA GAGATCATAT 
AGTTTCCAGG AGCCACCAGG AGAAAAAAGG ATTCAGGAAG AAGAATGGGA TGAATACGTC 
ATTCCTGCCA AATCCGAGTC TGAAAAGTAC AAAGTGAGCC GAACTTTCAG CTTCCTCATG 
AACAGGATGA CCAGCCCACG GAACAAATCT AAGATGAAAA ACAAGGACAC TAAAGAAAAA 
GAAAAAATGA ATCGACATCA GTTTGTCCCG GGAACGTTCT CCGGGGTTCT GCAGTGCTCC 
GGTTGTGATA AAACACTCCT GGGGAAAGAG TCACTCCAGT GTGCCAACTG TAAGGCAAAC 
ACACACAAGG GCTGCAAAGA TGCTGTGCCT CCGTGTACCA AGAAATTCCA AGAGAAATAC 
AACAAGAACA AACCACAGAG CATCCTTGGA AGCTCTTCAG TCAGAGACGT CCCAGCACCT 
GGGCTCTCCT TGCACCCTTC TTCCTCTATG CCCATCGGAC TGCCAGCTGG AAGGAAGGAA 
TTTGCGGCTC AGGTGCATCC ACTGTCCAGA AGTGTCCCCG GCACCACTTT GGAAAGCTTC 
AGGAGGGCAG TGACATCGTT AGAGTCTGAG GGTGACAGCT GGAGAAGCAG GTCTCATTCC 
GATGAGCTGT TCCAGTCTAT GGGCTCTTCT CCTTCCACGG AGTCATTCAT GATGGAAGAT 
GTTGTGGATT CTTCTCTGTG GATCGACCTC AGTAGTGATG CTCAGGAGTT TGAAGCAGAG 
TCTTGGAGTC TCGTGGTGGA CCCTTCCTTT TGCAGTAGGC AGGAGAAGGA TGTCATCAAA 
AGACAAGATG TCATTTTTGA GCTGATGCAG ACAGAGGTCC ACCACATCCA GACTCTGCTC 
ATCATGTCCG AGGTCTTCAG GAAAGGCATG AAGGAGGAAC TGCAGCTGGA CCACAGCACG 
GTGGACAAGA TCTTCCCCTG CTTAGATGAG CTGCTGGAGA CTCACCGGCA CTTCTTCTTC 
AGCATGAAGG AGCGCAGGCA GGAGTCCTGC GCTGGCAGTG ACAGGAACTT TGTGATCAAC 
CAAATTGGAG ACATTCTGGT ACAACAGTTT TCAGAGGAAA ATGCAAGCAA AATGAAGAGG 
ATCTATGGAG AATTCTGCAG CCATCACAAA GAAGCCATGA GTCTCTTTAA AGAGCTGCAG 
CAGAACAAGA AGTTTCAGAA TTTTATTAAG ATCCGAAATA GCAACCTGTT GGCTCGTCGC 
CGCGGAATCC CGGAGTGCAT CCTGCTAGTT ACCCAGCGCA TCACAAAGTA CCCAGTCTTG 
GTGGAAAGGA TCCTGCAGTA CACCAAGGAA AGGACTGAAG AGCACCGGGA CCTGTGCAAA 
GCTCTCGGCT TAATTAAAGA CATGATCGCA GCCGTGGACC TGAAAGTCAG CGAATACGAG 
AAGAACCAAA AATGGCTCGA GATCCTAAAT AAAATCGAGA ACAAAACATA CACAAAGCTC 
AAAAATGGCC ACGTGTTTAG AAAGCAGGCG CTGCTGAGCC AGGAACGGGC TCTCCTGCAT 
GATGGGCTTG TCTACTGGAA AACCGCTACG GGTCGCTTCA AAGACATCCT GGCTCTGCTA 
CTAACTGACG TGCTGCTCTT CTTACAAGAA AAAGACCAGA AATACATCTT TGCAGCTGTT 
GACCAGAAGC CATCAGTGAT TTCCCTCCAG AAGCTGATTG CTAGAGAAGT TGCCAACGAG 
GAGAGAGGCA TGTTTCTGAT CAGTGCTTCG TCAGCCGGCC CCGAGATGTA CGAAATCCAC 
ACCAATTCCA AGGAGGAGCG GAATAACTGG ATGAGGCGGA TCCAGCAGGC AGTAGAAAGT 
TGTCCAGAGG AAGAAGGAGG AAGGACGAGC GAGTCGGATG AAGAGAGGCG GAAAGCTGAA 
GCTAGAGTGG CCAAGATTCA GCAATGTCAA GAAATACTCA GTAACCAAGA CCAACAGATT 
TGCACGTACT TGGAAGAAAA GCTGCATATC TACGCTGAAC TTGGGGAACT GAGTGGCTTT 
GAGGATGTCC ACCTAGAACC CCATCTGCTC ATCAAACCTG ACCCTGGAGA GCCTCCCCAG 
GCAGCGTCCC TGCTTGCAGC AGCCCTGAGA GAAGCCGAGA GTCTGCAAGT GGCTGTGAAG 
GCTTCAAAGA TGGGCGATGT AAGCCAGTCA TCCGAAGAAA GCCCTGGAGG GACTGTCCTG 
ATGGACACAC CCAGTACACA GGATGTACCA GCGTCACCAA CTGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgef28 ( XM_017315362.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170851.1 Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X4, mRNA.

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CloneID	OMu78410
Clone ID Related Accession (Same CDS sequence)	XM_017315362.1 , XM_017315362.2
Accession Version	XM_017315362.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	rho guanine nucleotide exchange factor 28 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTTGA CTTCTGGAGC CTGTCGGCAC TGTACAGCCG TGATGTCCCT GAGGTACCCT 
GTGTTCCTGC ATGAAGATCG CTCCTTTGAT GGCCTCAAAA AATCCAAGCA TGTCCCAGCG 
TCCCTTGCTG CAGGCCAGCT CTCTGATGTT CTGAACGGGG GTGATGAAGT CTACGCTAAC 
TGTATGGTGA TTGACCAGGT TGGCGATTTG GATATCAACT ATATAAACCT AGAGGGACTC 
TCCACCCATA CGAGTCCCGA GTCTGGGAGG TCCATGCTGG GGCCTCAGGC TTGCATGCAC 
ACCCTTCCTC CCGACACCAG TCCCTGCGGG CGCCCACTCA TTGAGAACAG TGAGGGGACC 
CTGGATGCTG CAGCTTCGCA GTCTTTCGTG ACACCATCCA GTTCTCGCAC TTCCAACTTG 
AACCTTTCTT TTGGTCTCCA TGGATTTGAA AAGGAGCAGA GTCATCTCAA GAAAAGGAGT 
TCTAGCCTGG ATGCCCTGGT TGCCGACAGT GAGGGGGAAG GAGGTTCTGA GCCCCCCATC 
TGCTATGCTG TGGGCTCTCA GAGTTCCCCA AGAACTGGAC TCCCCAGCGG GGATGAACTG 
GACTCTTTTG AGACCAACAC TGAACCAGAC TGTAATATCT CCAGGACCGA ATCACTCTCT 
CTGTCCAGTA CTCTGCATTC AAAGGAGTCC TTGCTTTCTG GGATCCGGTC TCGCTCCTAC 
TCCTGCTCAT CACCCAAAAT CTCTTCAGGA AAATCCCGGC TAGTGCGCGA CTTCACAGTG 
TGCAGTACCA GCGAAGAACA GAGATCATAT AGTTTCCAGG AGCCACCAGG AGAAAAAAGG 
ATTCAGGAAG AAGAATGGGA TGAATACGTC ATTCCTGCCA AATCCGAGTC TGAAAAGTAC 
AAAGTGAGCC GAACTTTCAG CTTCCTCATG AACAGGATGA CCAGCCCACG GAACAAATCT 
AAGATGAAAA ACAAGGACAC TAAAGAAAAA GAAAAAATGA ATCGACATCA GTTTGTCCCG 
GGAACGTTCT CCGGGGTTCT GCAGTGCTCC GGTTGTGATA AAACACTCCT GGGGAAAGAG 
TCACTCCAGT GTGCCAACTG TAAGGCAAAC ACACACAAGG GCTGCAAAGA TGCTGTGCCT 
CCGTGTACCA AGAAATTCCA AGAGAAATAC AACAAGAACA AACCACAGAG CATCCTTGGA 
AGCTCTTCAG TCAGAGACGT CCCAGCACCT GGGCTCTCCT TGCACCCTTC TTCCTCTATG 
CCCATCGGAC TGCCAGCTGG AAGGAAGGAA TTTGCGGCTC AGGTGCATCC ACTGTCCAGA 
AGTGTCCCCG GCACCACTTT GGAAAGCTTC AGGAGGGCAG TGACATCGTT AGAGTCTGAG 
GGTGACAGCT GGAGAAGCAG GTCTCATTCC GATGAGCTGT TCCAGTCTAT GGGCTCTTCT 
CCTTCCACGG AGTCATTCAT GATGGAAGAT GTTGTGGATT CTTCTCTGTG GATCGACCTC 
AGTAGTGATG CTCAGGAGTT TGAAGCAGAG TCTTGGAGTC TCGTGGTGGA CCCTTCCTTT 
TGCAGTAGGC AGGAGAAGGA TGTCATCAAA AGACAAGATG TCATTTTTGA GCTGATGCAG 
ACAGAGGTCC ACCACATCCA GACTCTGCTC ATCATGTCCG AGGTCTTCAG GAAAGGCATG 
AAGGAGGAAC TGCAGCTGGA CCACAGCACG GTGGACAAGA TCTTCCCCTG CTTAGATGAG 
CTGCTGGAGA CTCACCGGCA CTTCTTCTTC AGCATGAAGG AGCGCAGGCA GGAGTCCTGC 
GCTGGCAGTG ACAGGAACTT TGTGATCAAC CAAATTGGAG ACATTCTGGT ACAACAGTTT 
TCAGAGGAAA ATGCAAGCAA AATGAAGAGG ATCTATGGAG AATTCTGCAG CCATCACAAA 
GAAGCCATGA GTCTCTTTAA AGAGCTGCAG CAGAACAAGA AGTTTCAGAA TTTTATTAAG 
ATCCGAAATA GCAACCTGTT GGCTCGTCGC CGCGGAATCC CGGAGTGCAT CCTGCTAGTT 
ACCCAGCGCA TCACAAAGTA CCCAGTCTTG GTGGAAAGGA TCCTGCAGTA CACCAAGGAA 
AGGACTGAAG AGCACCGGGA CCTGTGCAAA GCTCTCGGCT TAATTAAAGA CATGATCGCA 
GCCGTGGACC TGAAAGTCAG CGAATACGAG AAGAACCAAA AATGGCTCGA GATCCTAAAT 
AAAATCGAGA ACAAAACATA CACAAAGCTC AAAAATGGCC ACGTGTTTAG AAAGCAGGCG 
CTGCTGAGCC AGGAACGGGC TCTCCTGCAT GATGGGCTTG TCTACTGGAA AACCGCTACG 
GGTCGCTTCA AAGACATCCT GGCTCTGCTA CTAACTGACG TGCTGCTCTT CTTACAAGAA 
AAAGACCAGA AATACATCTT TGCAGCTGTT GACCAGAAGC CATCAGTGAT TTCCCTCCAG 
AAGCTGATTG CTAGAGAAGT TGCCAACGAG GAGAGAGGCA TGTTTCTGAT CAGTGCTTCG 
TCAGCCGGCC CCGAGATGTA CGAAATCCAC ACCAATTCCA AGGAGGAGCG GAATAACTGG 
ATGAGGCGGA TCCAGCAGGC AGTAGAAAGT TGTCCAGAGG AAGAAGGAGG AAGGACGAGC 
GAGTCGGATG AAGAGAGGCG GAAAGCTGAA GCTAGAGTGG CCAAGATTCA GCAATGTCAA 
GAAATACTCA GTAACCAAGA CCAACAGATT TGCACGTACT TGGAAGAAAA GCTGCATATC 
TACGCTGAAC TTGGGGAACT GAGTGGCTTT GAGGATGTCC ACCTAGAACC CCATCTGCTC 
ATCAAACCTG ACCCTGGAGA GCCTCCCCAG GCAGCGTCCC TGCTTGCAGC AGCCCTGAGA 
GAAGCCGAGA GTCTGCAAGT GGCTGTGAAG GCTTCAAAGA TGGGCGATGT AAGCCAGTCA 
TCCGAAGAAA GCCCTGGAGG GACTGTCCTG ATGGACACAC CCAGTACACA GGATGTACCA 
GCGTCACCAA CTGCTTCATT AGTCACAGAA GGGACAGAAG GAAGAGGCTG TTGGGATGTG 
GATCCCGGGC TCCAGGGTGT GGTAACCGAC TTGGCAGTCT CTGATGCAGG GGAGAAGGTG 
GAATATAGAA GTTTCTCCGG TTCTTCACAG TCAGAGATTA TACAAGCAAT ACAGAATTTA 
ACGCGTCTCC TCTACAGCCT TCAGGCTGCC TTGACCATCC AGGACAGTCA CATTGAGATC 
CACAAACTGG TTCTACAGCA GCGGGAGAGC TTGGCCCCTA GCCACTCCTT CCGAGGTGGC 
CCCTTGCAAG ACCAGGAGAA ATCCCGCTAC CTGGAAAAGC AGCGTGAAGA GCTGGCCAAC 
ATCCACAAGC TTCAACACCA GTTCCAGCAG GAGCAGCGGC GCTGGCACCG CACGTGCGAC 
CAGCAACAGC GCGAGCAGGA AGCCCAAGAG AGCTGGCTGC AGGCGCGGGA ACGTGAGTGC 
CAGTCCCAGG AGGAATTGTT GCTGCGCCAC CGTAGCGAGC TGGACCACCA ACTCCAGGAG 
TACCAGCAGA GCCTTGAGCG GCTACGGGAG GGCCAGCGGA TGGTGGAGAG GGAGAGACAG 
AAGATGCGTG TCCAGCAGGG TCTGCTGGGC CACTGCAAGC ATAGTCGCCA GCGGAGCCTC 
CCAGCCGTCT TCTCTCCAGG GAGCAAGGAG GTAACAGAAC TTAACCGAGC TGAGAGTTTG 
TGTCATGAAA ATTCATTTTT CATCAATGAA GCTTTTGGAC ACATGTCACT TAACACGTCC 
AATAAACCAA ACCCATCAGG GGTCCCCTGG GATGCTCACC CCCTGGAGGG GTCTCATTTT 
GATTTGGCAA GGACTAGTGA GAGTCCAACA GAACTGAAGA TAGACATTTC TCAGCCTCCT 
GATGTCAACT CTGAACTCTG GACAACGGGG CCGGGCCATC AGAGACCTGC TCTCCAAGAA 
AACAGCAAGG AGTCTTATAA AAATGTTGCA GATTTGGACT CCTTCCAGAG TGAATCGTCA 
TCTCCCCAGG ACTCCAATCA GCGTGGCCCC CAGCCACAGA CACTCACAAC AGAAGCAAAA 
CTAAGTCTTC CGATGGCGGC TGGACACGGT GGCGATGCTG GAGACGGGGC TGAAGAAAAC 
ATTCTTTACC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170851.1
CDS	91..4245
Translation	MFLTSGACRHCTAVMSLRYPVFLHEDRSFDGLKKSKHVPASLAAGQLSDVLNGGDEVYANCMVIDQVGDLDINYINLEGLSTHTSPESGRSMLGPQACMHTLPPDTSPCGRPLIENSEGTLDAAASQSFVTPSSSRTSNLNLSFGLHGFEKEQSHLKKRSSSLDALVADSEGEGGSEPPICYAVGSQSSPRTGLPSGDELDSFETNTEPDCNISRTESLSLSSTLHSKESLLSGIRSRSYSCSSPKISSGKSRLVRDFTVCSTSEEQRSYSFQEPPGEKRIQEEEWDEYVIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKMKNKDTKEKEKMNRHQFVPGTFSGVLQCSGCDKTLLGKESLQCANCKANTHKGCKDAVPPCTKKFQEKYNKNKPQSILGSSSVRDVPAPGLSLHPSSSMPIGLPAGRKEFAAQVHPLSRSVPGTTLESFRRAVTSLESEGDSWRSRSHSDELFQSMGSSPSTESFMMEDVVDSSLWIDLSSDAQEFEAESWSLVVDPSFCSRQEKDVIKRQDVIFELMQTEVHHIQTLLIMSEVFRKGMKEELQLDHSTVDKIFPCLDELLETHRHFFFSMKERRQESCAGSDRNFVINQIGDILVQQFSEENASKMKRIYGEFCSHHKEAMSLFKELQQNKKFQNFIKIRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKERTEEHRDLCKALGLIKDMIAAVDLKVSEYEKNQKWLEILNKIENKTYTKLKNGHVFRKQALLSQERALLHDGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNNWMRRIQQAVESCPEEEGGRTSESDEERRKAEARVAKIQQCQEILSNQDQQICTYLEEKLHIYAELGELSGFEDVHLEPHLLIKPDPGEPPQAASLLAAALREAESLQVAVKASKMGDVSQSSEESPGGTVLMDTPSTQDVPASPTASLVTEGTEGRGCWDVDPGLQGVVTDLAVSDAGEKVEYRSFSGSSQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIHKLVLQQRESLAPSHSFRGGPLQDQEKSRYLEKQREELANIHKLQHQFQQEQRRWHRTCDQQQREQEAQESWLQARERECQSQEELLLRHRSELDHQLQEYQQSLERLREGQRMVERERQKMRVQQGLLGHCKHSRQRSLPAVFSPGSKEVTELNRAESLCHENSFFINEAFGHMSLNTSNKPNPSGVPWDAHPLEGSHFDLARTSESPTELKIDISQPPDVNSELWTTGPGHQRPALQENSKESYKNVADLDSFQSESSSPQDSNQRGPQPQTLTTEAKLSLPMAAGHGGDAGDGAEENILYL

Target ORF information:

RefSeq Version	XM_017315362.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315362.1

ORF Insert Sequence:

ATGTTTTTGA CTTCTGGAGC CTGTCGGCAC TGTACAGCCG TGATGTCCCT GAGGTACCCT 
GTGTTCCTGC ATGAAGATCG CTCCTTTGAT GGCCTCAAAA AATCCAAGCA TGTCCCAGCG 
TCCCTTGCTG CAGGCCAGCT CTCTGATGTT CTGAACGGGG GTGATGAAGT CTACGCTAAC 
TGTATGGTGA TTGACCAGGT TGGCGATTTG GATATCAACT ATATAAACCT AGAGGGACTC 
TCCACCCATA CGAGTCCCGA GTCTGGGAGG TCCATGCTGG GGCCTCAGGC TTGCATGCAC 
ACCCTTCCTC CCGACACCAG TCCCTGCGGG CGCCCACTCA TTGAGAACAG TGAGGGGACC 
CTGGATGCTG CAGCTTCGCA GTCTTTCGTG ACACCATCCA GTTCTCGCAC TTCCAACTTG 
AACCTTTCTT TTGGTCTCCA TGGATTTGAA AAGGAGCAGA GTCATCTCAA GAAAAGGAGT 
TCTAGCCTGG ATGCCCTGGT TGCCGACAGT GAGGGGGAAG GAGGTTCTGA GCCCCCCATC 
TGCTATGCTG TGGGCTCTCA GAGTTCCCCA AGAACTGGAC TCCCCAGCGG GGATGAACTG 
GACTCTTTTG AGACCAACAC TGAACCAGAC TGTAATATCT CCAGGACCGA ATCACTCTCT 
CTGTCCAGTA CTCTGCATTC AAAGGAGTCC TTGCTTTCTG GGATCCGGTC TCGCTCCTAC 
TCCTGCTCAT CACCCAAAAT CTCTTCAGGA AAATCCCGGC TAGTGCGCGA CTTCACAGTG 
TGCAGTACCA GCGAAGAACA GAGATCATAT AGTTTCCAGG AGCCACCAGG AGAAAAAAGG 
ATTCAGGAAG AAGAATGGGA TGAATACGTC ATTCCTGCCA AATCCGAGTC TGAAAAGTAC 
AAAGTGAGCC GAACTTTCAG CTTCCTCATG AACAGGATGA CCAGCCCACG GAACAAATCT 
AAGATGAAAA ACAAGGACAC TAAAGAAAAA GAAAAAATGA ATCGACATCA GTTTGTCCCG 
GGAACGTTCT CCGGGGTTCT GCAGTGCTCC GGTTGTGATA AAACACTCCT GGGGAAAGAG 
TCACTCCAGT GTGCCAACTG TAAGGCAAAC ACACACAAGG GCTGCAAAGA TGCTGTGCCT 
CCGTGTACCA AGAAATTCCA AGAGAAATAC AACAAGAACA AACCACAGAG CATCCTTGGA 
AGCTCTTCAG TCAGAGACGT CCCAGCACCT GGGCTCTCCT TGCACCCTTC TTCCTCTATG 
CCCATCGGAC TGCCAGCTGG AAGGAAGGAA TTTGCGGCTC AGGTGCATCC ACTGTCCAGA 
AGTGTCCCCG GCACCACTTT GGAAAGCTTC AGGAGGGCAG TGACATCGTT AGAGTCTGAG 
GGTGACAGCT GGAGAAGCAG GTCTCATTCC GATGAGCTGT TCCAGTCTAT GGGCTCTTCT 
CCTTCCACGG AGTCATTCAT GATGGAAGAT GTTGTGGATT CTTCTCTGTG GATCGACCTC 
AGTAGTGATG CTCAGGAGTT TGAAGCAGAG TCTTGGAGTC TCGTGGTGGA CCCTTCCTTT 
TGCAGTAGGC AGGAGAAGGA TGTCATCAAA AGACAAGATG TCATTTTTGA GCTGATGCAG 
ACAGAGGTCC ACCACATCCA GACTCTGCTC ATCATGTCCG AGGTCTTCAG GAAAGGCATG 
AAGGAGGAAC TGCAGCTGGA CCACAGCACG GTGGACAAGA TCTTCCCCTG CTTAGATGAG 
CTGCTGGAGA CTCACCGGCA CTTCTTCTTC AGCATGAAGG AGCGCAGGCA GGAGTCCTGC 
GCTGGCAGTG ACAGGAACTT TGTGATCAAC CAAATTGGAG ACATTCTGGT ACAACAGTTT 
TCAGAGGAAA ATGCAAGCAA AATGAAGAGG ATCTATGGAG AATTCTGCAG CCATCACAAA 
GAAGCCATGA GTCTCTTTAA AGAGCTGCAG CAGAACAAGA AGTTTCAGAA TTTTATTAAG 
ATCCGAAATA GCAACCTGTT GGCTCGTCGC CGCGGAATCC CGGAGTGCAT CCTGCTAGTT 
ACCCAGCGCA TCACAAAGTA CCCAGTCTTG GTGGAAAGGA TCCTGCAGTA CACCAAGGAA 
AGGACTGAAG AGCACCGGGA CCTGTGCAAA GCTCTCGGCT TAATTAAAGA CATGATCGCA 
GCCGTGGACC TGAAAGTCAG CGAATACGAG AAGAACCAAA AATGGCTCGA GATCCTAAAT 
AAAATCGAGA ACAAAACATA CACAAAGCTC AAAAATGGCC ACGTGTTTAG AAAGCAGGCG 
CTGCTGAGCC AGGAACGGGC TCTCCTGCAT GATGGGCTTG TCTACTGGAA AACCGCTACG 
GGTCGCTTCA AAGACATCCT GGCTCTGCTA CTAACTGACG TGCTGCTCTT CTTACAAGAA 
AAAGACCAGA AATACATCTT TGCAGCTGTT GACCAGAAGC CATCAGTGAT TTCCCTCCAG 
AAGCTGATTG CTAGAGAAGT TGCCAACGAG GAGAGAGGCA TGTTTCTGAT CAGTGCTTCG 
TCAGCCGGCC CCGAGATGTA CGAAATCCAC ACCAATTCCA AGGAGGAGCG GAATAACTGG 
ATGAGGCGGA TCCAGCAGGC AGTAGAAAGT TGTCCAGAGG AAGAAGGAGG AAGGACGAGC 
GAGTCGGATG AAGAGAGGCG GAAAGCTGAA GCTAGAGTGG CCAAGATTCA GCAATGTCAA 
GAAATACTCA GTAACCAAGA CCAACAGATT TGCACGTACT TGGAAGAAAA GCTGCATATC 
TACGCTGAAC TTGGGGAACT GAGTGGCTTT GAGGATGTCC ACCTAGAACC CCATCTGCTC 
ATCAAACCTG ACCCTGGAGA GCCTCCCCAG GCAGCGTCCC TGCTTGCAGC AGCCCTGAGA 
GAAGCCGAGA GTCTGCAAGT GGCTGTGAAG GCTTCAAAGA TGGGCGATGT AAGCCAGTCA 
TCCGAAGAAA GCCCTGGAGG GACTGTCCTG ATGGACACAC CCAGTACACA GGATGTACCA 
GCGTCACCAA CTGCTTCATT AGTCACAGAA GGGACAGAAG GAAGAGGCTG TTGGGATGTG 
GATCCCGGGC TCCAGGGTGT GGTAACCGAC TTGGCAGTCT CTGATGCAGG GGAGAAGGTG 
GAATATAGAA GTTTCTCCGG TTCTTCACAG TCAGAGATTA TACAAGCAAT ACAGAATTTA 
ACGCGTCTCC TCTACAGCCT TCAGGCTGCC TTGACCATCC AGGACAGTCA CATTGAGATC 
CACAAACTGG TTCTACAGCA GCGGGAGAGC TTGGCCCCTA GCCACTCCTT CCGAGGTGGC 
CCCTTGCAAG ACCAGGAGAA ATCCCGCTAC CTGGAAAAGC AGCGTGAAGA GCTGGCCAAC 
ATCCACAAGC TTCAACACCA GTTCCAGCAG GAGCAGCGGC GCTGGCACCG CACGTGCGAC 
CAGCAACAGC GCGAGCAGGA AGCCCAAGAG AGCTGGCTGC AGGCGCGGGA ACGTGAGTGC 
CAGTCCCAGG AGGAATTGTT GCTGCGCCAC CGTAGCGAGC TGGACCACCA ACTCCAGGAG 
TACCAGCAGA GCCTTGAGCG GCTACGGGAG GGCCAGCGGA TGGTGGAGAG GGAGAGACAG 
AAGATGCGTG TCCAGCAGGG TCTGCTGGGC CACTGCAAGC ATAGTCGCCA GCGGAGCCTC 
CCAGCCGTCT TCTCTCCAGG GAGCAAGGAG GTAACAGAAC TTAACCGAGC TGAGAGTTTG 
TGTCATGAAA ATTCATTTTT CATCAATGAA GCTTTTGGAC ACATGTCACT TAACACGTCC 
AATAAACCAA ACCCATCAGG GGTCCCCTGG GATGCTCACC CCCTGGAGGG GTCTCATTTT 
GATTTGGCAA GGACTAGTGA GAGTCCAACA GAACTGAAGA TAGACATTTC TCAGCCTCCT 
GATGTCAACT CTGAACTCTG GACAACGGGG CCGGGCCATC AGAGACCTGC TCTCCAAGAA 
AACAGCAAGG AGTCTTATAA AAATGTTGCA GATTTGGACT CCTTCCAGAG TGAATCGTCA 
TCTCCCCAGG ACTCCAATCA GCGTGGCCCC CAGCCACAGA CACTCACAAC AGAAGCAAAA 
CTAAGTCTTC CGATGGCGGC TGGACACGGT GGCGATGCTG GAGACGGGGC TGAAGAAAAC 
ATTCTTTACC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgef28 ( XM_017315362.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170851.1 Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X5, mRNA.

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CloneID	OMu78410
Clone ID Related Accession (Same CDS sequence)	XM_017315362.1 , XM_017315362.2
Accession Version	XM_017315362.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rho guanine nucleotide exchange factor 28 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017315362.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTTGA CTTCTGGAGC CTGTCGGCAC TGTACAGCCG TGATGTCCCT GAGGTACCCT 
GTGTTCCTGC ATGAAGATCG CTCCTTTGAT GGCCTCAAAA AATCCAAGCA TGTCCCAGCG 
TCCCTTGCTG CAGGCCAGCT CTCTGATGTT CTGAACGGGG GTGATGAAGT CTACGCTAAC 
TGTATGGTGA TTGACCAGGT TGGCGATTTG GATATCAACT ATATAAACCT AGAGGGACTC 
TCCACCCATA CGAGTCCCGA GTCTGGGAGG TCCATGCTGG GGCCTCAGGC TTGCATGCAC 
ACCCTTCCTC CCGACACCAG TCCCTGCGGG CGCCCACTCA TTGAGAACAG TGAGGGGACC 
CTGGATGCTG CAGCTTCGCA GTCTTTCGTG ACACCATCCA GTTCTCGCAC TTCCAACTTG 
AACCTTTCTT TTGGTCTCCA TGGATTTGAA AAGGAGCAGA GTCATCTCAA GAAAAGGAGT 
TCTAGCCTGG ATGCCCTGGT TGCCGACAGT GAGGGGGAAG GAGGTTCTGA GCCCCCCATC 
TGCTATGCTG TGGGCTCTCA GAGTTCCCCA AGAACTGGAC TCCCCAGCGG GGATGAACTG 
GACTCTTTTG AGACCAACAC TGAACCAGAC TGTAATATCT CCAGGACCGA ATCACTCTCT 
CTGTCCAGTA CTCTGCATTC AAAGGAGTCC TTGCTTTCTG GGATCCGGTC TCGCTCCTAC 
TCCTGCTCAT CACCCAAAAT CTCTTCAGGA AAATCCCGGC TAGTGCGCGA CTTCACAGTG 
TGCAGTACCA GCGAAGAACA GAGATCATAT AGTTTCCAGG AGCCACCAGG AGAAAAAAGG 
ATTCAGGAAG AAGAATGGGA TGAATACGTC ATTCCTGCCA AATCCGAGTC TGAAAAGTAC 
AAAGTGAGCC GAACTTTCAG CTTCCTCATG AACAGGATGA CCAGCCCACG GAACAAATCT 
AAGATGAAAA ACAAGGACAC TAAAGAAAAA GAAAAAATGA ATCGACATCA GTTTGTCCCG 
GGAACGTTCT CCGGGGTTCT GCAGTGCTCC GGTTGTGATA AAACACTCCT GGGGAAAGAG 
TCACTCCAGT GTGCCAACTG TAAGGCAAAC ACACACAAGG GCTGCAAAGA TGCTGTGCCT 
CCGTGTACCA AGAAATTCCA AGAGAAATAC AACAAGAACA AACCACAGAG CATCCTTGGA 
AGCTCTTCAG TCAGAGACGT CCCAGCACCT GGGCTCTCCT TGCACCCTTC TTCCTCTATG 
CCCATCGGAC TGCCAGCTGG AAGGAAGGAA TTTGCGGCTC AGGTGCATCC ACTGTCCAGA 
AGTGTCCCCG GCACCACTTT GGAAAGCTTC AGGAGGGCAG TGACATCGTT AGAGTCTGAG 
GGTGACAGCT GGAGAAGCAG GTCTCATTCC GATGAGCTGT TCCAGTCTAT GGGCTCTTCT 
CCTTCCACGG AGTCATTCAT GATGGAAGAT GTTGTGGATT CTTCTCTGTG GATCGACCTC 
AGTAGTGATG CTCAGGAGTT TGAAGCAGAG TCTTGGAGTC TCGTGGTGGA CCCTTCCTTT 
TGCAGTAGGC AGGAGAAGGA TGTCATCAAA AGACAAGATG TCATTTTTGA GCTGATGCAG 
ACAGAGGTCC ACCACATCCA GACTCTGCTC ATCATGTCCG AGGTCTTCAG GAAAGGCATG 
AAGGAGGAAC TGCAGCTGGA CCACAGCACG GTGGACAAGA TCTTCCCCTG CTTAGATGAG 
CTGCTGGAGA CTCACCGGCA CTTCTTCTTC AGCATGAAGG AGCGCAGGCA GGAGTCCTGC 
GCTGGCAGTG ACAGGAACTT TGTGATCAAC CAAATTGGAG ACATTCTGGT ACAACAGTTT 
TCAGAGGAAA ATGCAAGCAA AATGAAGAGG ATCTATGGAG AATTCTGCAG CCATCACAAA 
GAAGCCATGA GTCTCTTTAA AGAGCTGCAG CAGAACAAGA AGTTTCAGAA TTTTATTAAG 
ATCCGAAATA GCAACCTGTT GGCTCGTCGC CGCGGAATCC CGGAGTGCAT CCTGCTAGTT 
ACCCAGCGCA TCACAAAGTA CCCAGTCTTG GTGGAAAGGA TCCTGCAGTA CACCAAGGAA 
AGGACTGAAG AGCACCGGGA CCTGTGCAAA GCTCTCGGCT TAATTAAAGA CATGATCGCA 
GCCGTGGACC TGAAAGTCAG CGAATACGAG AAGAACCAAA AATGGCTCGA GATCCTAAAT 
AAAATCGAGA ACAAAACATA CACAAAGCTC AAAAATGGCC ACGTGTTTAG AAAGCAGGCG 
CTGCTGAGCC AGGAACGGGC TCTCCTGCAT GATGGGCTTG TCTACTGGAA AACCGCTACG 
GGTCGCTTCA AAGACATCCT GGCTCTGCTA CTAACTGACG TGCTGCTCTT CTTACAAGAA 
AAAGACCAGA AATACATCTT TGCAGCTGTT GACCAGAAGC CATCAGTGAT TTCCCTCCAG 
AAGCTGATTG CTAGAGAAGT TGCCAACGAG GAGAGAGGCA TGTTTCTGAT CAGTGCTTCG 
TCAGCCGGCC CCGAGATGTA CGAAATCCAC ACCAATTCCA AGGAGGAGCG GAATAACTGG 
ATGAGGCGGA TCCAGCAGGC AGTAGAAAGT TGTCCAGAGG AAGAAGGAGG AAGGACGAGC 
GAGTCGGATG AAGAGAGGCG GAAAGCTGAA GCTAGAGTGG CCAAGATTCA GCAATGTCAA 
GAAATACTCA GTAACCAAGA CCAACAGATT TGCACGTACT TGGAAGAAAA GCTGCATATC 
TACGCTGAAC TTGGGGAACT GAGTGGCTTT GAGGATGTCC ACCTAGAACC CCATCTGCTC 
ATCAAACCTG ACCCTGGAGA GCCTCCCCAG GCAGCGTCCC TGCTTGCAGC AGCCCTGAGA 
GAAGCCGAGA GTCTGCAAGT GGCTGTGAAG GCTTCAAAGA TGGGCGATGT AAGCCAGTCA 
TCCGAAGAAA GCCCTGGAGG GACTGTCCTG ATGGACACAC CCAGTACACA GGATGTACCA 
GCGTCACCAA CTGCTTCATT AGTCACAGAA GGGACAGAAG GAAGAGGCTG TTGGGATGTG 
GATCCCGGGC TCCAGGGTGT GGTAACCGAC TTGGCAGTCT CTGATGCAGG GGAGAAGGTG 
GAATATAGAA GTTTCTCCGG TTCTTCACAG TCAGAGATTA TACAAGCAAT ACAGAATTTA 
ACGCGTCTCC TCTACAGCCT TCAGGCTGCC TTGACCATCC AGGACAGTCA CATTGAGATC 
CACAAACTGG TTCTACAGCA GCGGGAGAGC TTGGCCCCTA GCCACTCCTT CCGAGGTGGC 
CCCTTGCAAG ACCAGGAGAA ATCCCGCTAC CTGGAAAAGC AGCGTGAAGA GCTGGCCAAC 
ATCCACAAGC TTCAACACCA GTTCCAGCAG GAGCAGCGGC GCTGGCACCG CACGTGCGAC 
CAGCAACAGC GCGAGCAGGA AGCCCAAGAG AGCTGGCTGC AGGCGCGGGA ACGTGAGTGC 
CAGTCCCAGG AGGAATTGTT GCTGCGCCAC CGTAGCGAGC TGGACCACCA ACTCCAGGAG 
TACCAGCAGA GCCTTGAGCG GCTACGGGAG GGCCAGCGGA TGGTGGAGAG GGAGAGACAG 
AAGATGCGTG TCCAGCAGGG TCTGCTGGGC CACTGCAAGC ATAGTCGCCA GCGGAGCCTC 
CCAGCCGTCT TCTCTCCAGG GAGCAAGGAG GTAACAGAAC TTAACCGAGC TGAGAGTTTG 
TGTCATGAAA ATTCATTTTT CATCAATGAA GCTTTTGGAC ACATGTCACT TAACACGTCC 
AATAAACCAA ACCCATCAGG GGTCCCCTGG GATGCTCACC CCCTGGAGGG GTCTCATTTT 
GATTTGGCAA GGACTAGTGA GAGTCCAACA GAACTGAAGA TAGACATTTC TCAGCCTCCT 
GATGTCAACT CTGAACTCTG GACAACGGGG CCGGGCCATC AGAGACCTGC TCTCCAAGAA 
AACAGCAAGG AGTCTTATAA AAATGTTGCA GATTTGGACT CCTTCCAGAG TGAATCGTCA 
TCTCCCCAGG ACTCCAATCA GCGTGGCCCC CAGCCACAGA CACTCACAAC AGAAGCAAAA 
CTAAGTCTTC CGATGGCGGC TGGACACGGT GGCGATGCTG GAGACGGGGC TGAAGAAAAC 
ATTCTTTACC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170851.1
CDS	219..4373
Translation	MFLTSGACRHCTAVMSLRYPVFLHEDRSFDGLKKSKHVPASLAAGQLSDVLNGGDEVYANCMVIDQVGDLDINYINLEGLSTHTSPESGRSMLGPQACMHTLPPDTSPCGRPLIENSEGTLDAAASQSFVTPSSSRTSNLNLSFGLHGFEKEQSHLKKRSSSLDALVADSEGEGGSEPPICYAVGSQSSPRTGLPSGDELDSFETNTEPDCNISRTESLSLSSTLHSKESLLSGIRSRSYSCSSPKISSGKSRLVRDFTVCSTSEEQRSYSFQEPPGEKRIQEEEWDEYVIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKMKNKDTKEKEKMNRHQFVPGTFSGVLQCSGCDKTLLGKESLQCANCKANTHKGCKDAVPPCTKKFQEKYNKNKPQSILGSSSVRDVPAPGLSLHPSSSMPIGLPAGRKEFAAQVHPLSRSVPGTTLESFRRAVTSLESEGDSWRSRSHSDELFQSMGSSPSTESFMMEDVVDSSLWIDLSSDAQEFEAESWSLVVDPSFCSRQEKDVIKRQDVIFELMQTEVHHIQTLLIMSEVFRKGMKEELQLDHSTVDKIFPCLDELLETHRHFFFSMKERRQESCAGSDRNFVINQIGDILVQQFSEENASKMKRIYGEFCSHHKEAMSLFKELQQNKKFQNFIKIRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKERTEEHRDLCKALGLIKDMIAAVDLKVSEYEKNQKWLEILNKIENKTYTKLKNGHVFRKQALLSQERALLHDGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNNWMRRIQQAVESCPEEEGGRTSESDEERRKAEARVAKIQQCQEILSNQDQQICTYLEEKLHIYAELGELSGFEDVHLEPHLLIKPDPGEPPQAASLLAAALREAESLQVAVKASKMGDVSQSSEESPGGTVLMDTPSTQDVPASPTASLVTEGTEGRGCWDVDPGLQGVVTDLAVSDAGEKVEYRSFSGSSQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIHKLVLQQRESLAPSHSFRGGPLQDQEKSRYLEKQREELANIHKLQHQFQQEQRRWHRTCDQQQREQEAQESWLQARERECQSQEELLLRHRSELDHQLQEYQQSLERLREGQRMVERERQKMRVQQGLLGHCKHSRQRSLPAVFSPGSKEVTELNRAESLCHENSFFINEAFGHMSLNTSNKPNPSGVPWDAHPLEGSHFDLARTSESPTELKIDISQPPDVNSELWTTGPGHQRPALQENSKESYKNVADLDSFQSESSSPQDSNQRGPQPQTLTTEAKLSLPMAAGHGGDAGDGAEENILYL

Target ORF information:

RefSeq Version	XM_017315362.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315362.2

ORF Insert Sequence:

ATGTTTTTGA CTTCTGGAGC CTGTCGGCAC TGTACAGCCG TGATGTCCCT GAGGTACCCT 
GTGTTCCTGC ATGAAGATCG CTCCTTTGAT GGCCTCAAAA AATCCAAGCA TGTCCCAGCG 
TCCCTTGCTG CAGGCCAGCT CTCTGATGTT CTGAACGGGG GTGATGAAGT CTACGCTAAC 
TGTATGGTGA TTGACCAGGT TGGCGATTTG GATATCAACT ATATAAACCT AGAGGGACTC 
TCCACCCATA CGAGTCCCGA GTCTGGGAGG TCCATGCTGG GGCCTCAGGC TTGCATGCAC 
ACCCTTCCTC CCGACACCAG TCCCTGCGGG CGCCCACTCA TTGAGAACAG TGAGGGGACC 
CTGGATGCTG CAGCTTCGCA GTCTTTCGTG ACACCATCCA GTTCTCGCAC TTCCAACTTG 
AACCTTTCTT TTGGTCTCCA TGGATTTGAA AAGGAGCAGA GTCATCTCAA GAAAAGGAGT 
TCTAGCCTGG ATGCCCTGGT TGCCGACAGT GAGGGGGAAG GAGGTTCTGA GCCCCCCATC 
TGCTATGCTG TGGGCTCTCA GAGTTCCCCA AGAACTGGAC TCCCCAGCGG GGATGAACTG 
GACTCTTTTG AGACCAACAC TGAACCAGAC TGTAATATCT CCAGGACCGA ATCACTCTCT 
CTGTCCAGTA CTCTGCATTC AAAGGAGTCC TTGCTTTCTG GGATCCGGTC TCGCTCCTAC 
TCCTGCTCAT CACCCAAAAT CTCTTCAGGA AAATCCCGGC TAGTGCGCGA CTTCACAGTG 
TGCAGTACCA GCGAAGAACA GAGATCATAT AGTTTCCAGG AGCCACCAGG AGAAAAAAGG 
ATTCAGGAAG AAGAATGGGA TGAATACGTC ATTCCTGCCA AATCCGAGTC TGAAAAGTAC 
AAAGTGAGCC GAACTTTCAG CTTCCTCATG AACAGGATGA CCAGCCCACG GAACAAATCT 
AAGATGAAAA ACAAGGACAC TAAAGAAAAA GAAAAAATGA ATCGACATCA GTTTGTCCCG 
GGAACGTTCT CCGGGGTTCT GCAGTGCTCC GGTTGTGATA AAACACTCCT GGGGAAAGAG 
TCACTCCAGT GTGCCAACTG TAAGGCAAAC ACACACAAGG GCTGCAAAGA TGCTGTGCCT 
CCGTGTACCA AGAAATTCCA AGAGAAATAC AACAAGAACA AACCACAGAG CATCCTTGGA 
AGCTCTTCAG TCAGAGACGT CCCAGCACCT GGGCTCTCCT TGCACCCTTC TTCCTCTATG 
CCCATCGGAC TGCCAGCTGG AAGGAAGGAA TTTGCGGCTC AGGTGCATCC ACTGTCCAGA 
AGTGTCCCCG GCACCACTTT GGAAAGCTTC AGGAGGGCAG TGACATCGTT AGAGTCTGAG 
GGTGACAGCT GGAGAAGCAG GTCTCATTCC GATGAGCTGT TCCAGTCTAT GGGCTCTTCT 
CCTTCCACGG AGTCATTCAT GATGGAAGAT GTTGTGGATT CTTCTCTGTG GATCGACCTC 
AGTAGTGATG CTCAGGAGTT TGAAGCAGAG TCTTGGAGTC TCGTGGTGGA CCCTTCCTTT 
TGCAGTAGGC AGGAGAAGGA TGTCATCAAA AGACAAGATG TCATTTTTGA GCTGATGCAG 
ACAGAGGTCC ACCACATCCA GACTCTGCTC ATCATGTCCG AGGTCTTCAG GAAAGGCATG 
AAGGAGGAAC TGCAGCTGGA CCACAGCACG GTGGACAAGA TCTTCCCCTG CTTAGATGAG 
CTGCTGGAGA CTCACCGGCA CTTCTTCTTC AGCATGAAGG AGCGCAGGCA GGAGTCCTGC 
GCTGGCAGTG ACAGGAACTT TGTGATCAAC CAAATTGGAG ACATTCTGGT ACAACAGTTT 
TCAGAGGAAA ATGCAAGCAA AATGAAGAGG ATCTATGGAG AATTCTGCAG CCATCACAAA 
GAAGCCATGA GTCTCTTTAA AGAGCTGCAG CAGAACAAGA AGTTTCAGAA TTTTATTAAG 
ATCCGAAATA GCAACCTGTT GGCTCGTCGC CGCGGAATCC CGGAGTGCAT CCTGCTAGTT 
ACCCAGCGCA TCACAAAGTA CCCAGTCTTG GTGGAAAGGA TCCTGCAGTA CACCAAGGAA 
AGGACTGAAG AGCACCGGGA CCTGTGCAAA GCTCTCGGCT TAATTAAAGA CATGATCGCA 
GCCGTGGACC TGAAAGTCAG CGAATACGAG AAGAACCAAA AATGGCTCGA GATCCTAAAT 
AAAATCGAGA ACAAAACATA CACAAAGCTC AAAAATGGCC ACGTGTTTAG AAAGCAGGCG 
CTGCTGAGCC AGGAACGGGC TCTCCTGCAT GATGGGCTTG TCTACTGGAA AACCGCTACG 
GGTCGCTTCA AAGACATCCT GGCTCTGCTA CTAACTGACG TGCTGCTCTT CTTACAAGAA 
AAAGACCAGA AATACATCTT TGCAGCTGTT GACCAGAAGC CATCAGTGAT TTCCCTCCAG 
AAGCTGATTG CTAGAGAAGT TGCCAACGAG GAGAGAGGCA TGTTTCTGAT CAGTGCTTCG 
TCAGCCGGCC CCGAGATGTA CGAAATCCAC ACCAATTCCA AGGAGGAGCG GAATAACTGG 
ATGAGGCGGA TCCAGCAGGC AGTAGAAAGT TGTCCAGAGG AAGAAGGAGG AAGGACGAGC 
GAGTCGGATG AAGAGAGGCG GAAAGCTGAA GCTAGAGTGG CCAAGATTCA GCAATGTCAA 
GAAATACTCA GTAACCAAGA CCAACAGATT TGCACGTACT TGGAAGAAAA GCTGCATATC 
TACGCTGAAC TTGGGGAACT GAGTGGCTTT GAGGATGTCC ACCTAGAACC CCATCTGCTC 
ATCAAACCTG ACCCTGGAGA GCCTCCCCAG GCAGCGTCCC TGCTTGCAGC AGCCCTGAGA 
GAAGCCGAGA GTCTGCAAGT GGCTGTGAAG GCTTCAAAGA TGGGCGATGT AAGCCAGTCA 
TCCGAAGAAA GCCCTGGAGG GACTGTCCTG ATGGACACAC CCAGTACACA GGATGTACCA 
GCGTCACCAA CTGCTTCATT AGTCACAGAA GGGACAGAAG GAAGAGGCTG TTGGGATGTG 
GATCCCGGGC TCCAGGGTGT GGTAACCGAC TTGGCAGTCT CTGATGCAGG GGAGAAGGTG 
GAATATAGAA GTTTCTCCGG TTCTTCACAG TCAGAGATTA TACAAGCAAT ACAGAATTTA 
ACGCGTCTCC TCTACAGCCT TCAGGCTGCC TTGACCATCC AGGACAGTCA CATTGAGATC 
CACAAACTGG TTCTACAGCA GCGGGAGAGC TTGGCCCCTA GCCACTCCTT CCGAGGTGGC 
CCCTTGCAAG ACCAGGAGAA ATCCCGCTAC CTGGAAAAGC AGCGTGAAGA GCTGGCCAAC 
ATCCACAAGC TTCAACACCA GTTCCAGCAG GAGCAGCGGC GCTGGCACCG CACGTGCGAC 
CAGCAACAGC GCGAGCAGGA AGCCCAAGAG AGCTGGCTGC AGGCGCGGGA ACGTGAGTGC 
CAGTCCCAGG AGGAATTGTT GCTGCGCCAC CGTAGCGAGC TGGACCACCA ACTCCAGGAG 
TACCAGCAGA GCCTTGAGCG GCTACGGGAG GGCCAGCGGA TGGTGGAGAG GGAGAGACAG 
AAGATGCGTG TCCAGCAGGG TCTGCTGGGC CACTGCAAGC ATAGTCGCCA GCGGAGCCTC 
CCAGCCGTCT TCTCTCCAGG GAGCAAGGAG GTAACAGAAC TTAACCGAGC TGAGAGTTTG 
TGTCATGAAA ATTCATTTTT CATCAATGAA GCTTTTGGAC ACATGTCACT TAACACGTCC 
AATAAACCAA ACCCATCAGG GGTCCCCTGG GATGCTCACC CCCTGGAGGG GTCTCATTTT 
GATTTGGCAA GGACTAGTGA GAGTCCAACA GAACTGAAGA TAGACATTTC TCAGCCTCCT 
GATGTCAACT CTGAACTCTG GACAACGGGG CCGGGCCATC AGAGACCTGC TCTCCAAGAA 
AACAGCAAGG AGTCTTATAA AAATGTTGCA GATTTGGACT CCTTCCAGAG TGAATCGTCA 
TCTCCCCAGG ACTCCAATCA GCGTGGCCCC CAGCCACAGA CACTCACAAC AGAAGCAAAA 
CTAAGTCTTC CGATGGCGGC TGGACACGGT GGCGATGCTG GAGACGGGGC TGAAGAAAAC 
ATTCTTTACC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgef28 ( XM_030247128.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030102988.1 Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X3, mRNA.

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CloneID	OMu86213
Clone ID Related Accession (Same CDS sequence)	XM_030247128.1
Accession Version	XM_030247128.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rho guanine nucleotide exchange factor 28 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTCTG GAGCTGCAGA AACTGAAGAA GAGGTTAGGA ATTTGGAGTC TGGCAGATCA 
CCATCGGAGG AGGAGGAGGA TGCAAAGAGC ATCAAAAGCC AGGTGGATGG GCCGAGTGAA 
CACGAAGACC AGGACCGCCT ACCTCTGGAT CGCTCCTTTG ATGGCCTCAA AAAATCCAAG 
CATGTCCCAG CGTCCCTTGC TGCAGGCCAG CTCTCTGATG TTCTGAACGG GGGTGATGAA 
GTCTACGCTA ACTGTATGGT GATTGACCAG GTTGGCGATT TGGATATCAA CTATATAAAC 
CTAGAGGGAC TCTCCACCCA TACGAGTCCC GAGTCTGGGA GGTCCATGCT GGGGCCTCAG 
GCTTGCATGC ACACCCTTCC TCCCGACACC AGTCCCTGCG GGCGCCCACT CATTGAGAAC 
AGTGAGGGGA CCCTGGATGC TGCAGCTTCG CAGTCTTTCG TGACACCATC CAGTTCTCGC 
ACTTCCAACT TGAACCTTTC TTTTGGTCTC CATGGATTTG AAAAGGAGCA GAGTCATCTC 
AAGAAAAGGA GTTCTAGCCT GGATGCCCTG GTTGCCGACA GTGAGGGGGA AGGAGGTTCT 
GAGCCCCCCA TCTGCTATGC TGTGGGCTCT CAGAGTTCCC CAAGAACTGG ACTCCCCAGC 
GGGGATGAAC TGGACTCTTT TGAGACCAAC ACTGAACCAG ACTGTAATAT CTCCAGGACC 
GAATCACTCT CTCTGTCCAG TACTCTGCAT TCAAAGGAGT CCTTGCTTTC TGGGATCCGG 
TCTCGCTCCT ACTCCTGCTC ATCACCCAAA ATCTCTTCAG GAAAATCCCG GCTAGTGCGC 
GACTTCACAG TGTGCAGTAC CAGCGAAGAA CAGAGATCAT ATAGTTTCCA GGAGCCACCA 
GGAGAAAAAA GGATTCAGGA AGAAGAATGG GATGAATACG TCATTCCTGC CAAATCCGAG 
TCTGAAAAGT ACAAAGTGAG CCGAACTTTC AGCTTCCTCA TGAACAGGAT GACCAGCCCA 
CGGAACAAAT CTAAGATGAA AAACAAGGAC ACTAAAGAAA AAGAAAAAAT GAATCGACAT 
CAGTTTGTCC CGGGAACGTT CTCCGGGGTT CTGCAGTGCT CCGGTTGTGA TAAAACACTC 
CTGGGGAAAG AGTCACTCCA GTGTGCCAAC TGTAAGGCAA ACACACACAA GGGCTGCAAA 
GATGCTGTGC CTCCGTGTAC CAAGAAATTC CAAGAGAAAT ACAACAAGAA CAAACCACAG 
AGCATCCTTG GAAGCTCTTC AGTCAGAGAC GTCCCAGCAC CTGGGCTCTC CTTGCACCCT 
TCTTCCTCTA TGCCCATCGG ACTGCCAGCT GGAAGGAAGG AATTTGCGGC TCAGGTGCAT 
CCACTGTCCA GAAGTGTCCC CGGCACCACT TTGGAAAGCT TCAGGAGGGC AGTGACATCG 
TTAGAGTCTG AGGGTGACAG CTGGAGAAGC AGGTCTCATT CCGATGAGCT GTTCCAGTCT 
ATGGGCTCTT CTCCTTCCAC GGAGTCATTC ATGATGGAAG ATGTTGTGGA TTCTTCTCTG 
TGGATCGACC TCAGTAGTGA TGCTCAGGAG TTTGAAGCAG AGTCTTGGAG TCTCGTGGTG 
GACCCTTCCT TTTGCAGTAG GCAGGAGAAG GATGTCATCA AAAGACAAGA TGTCATTTTT 
GAGCTGATGC AGACAGAGGT CCACCACATC CAGACTCTGC TCATCATGTC CGAGGTCTTC 
AGGAAAGGCA TGAAGGAGGA ACTGCAGCTG GACCACAGCA CGGTGGACAA GATCTTCCCC 
TGCTTAGATG AGCTGCTGGA GACTCACCGG CACTTCTTCT TCAGCATGAA GGAGCGCAGG 
CAGGAGTCCT GCGCTGGCAG TGACAGGAAC TTTGTGATCA ACCAAATTGG AGACATTCTG 
GTACAACAGT TTTCAGAGGA AAATGCAAGC AAAATGAAGA GGATCTATGG AGAATTCTGC 
AGCCATCACA AAGAAGCCAT GAGTCTCTTT AAAGAGCTGC AGCAGAACAA GAAGTTTCAG 
AATTTTATTA AGATCCGAAA TAGCAACCTG TTGGCTCGTC GCCGCGGAAT CCCGGAGTGC 
ATCCTGCTAG TTACCCAGCG CATCACAAAG TACCCAGTCT TGGTGGAAAG GATCCTGCAG 
TACACCAAGG AAAGGACTGA AGAGCACCGG GACCTGTGCA AAGCTCTCGG CTTAATTAAA 
GACATGATCG CAGCCGTGGA CCTGAAAGTC AGCGAATACG AGAAGAACCA AAAATGGCTC 
GAGATCCTAA ATAAAATCGA GAACAAAACA TACACAAAGC TCAAAAATGG CCACGTGTTT 
AGAAAGCAGG CGCTGCTGAG CCAGGAACGG GCTCTCCTGC ATGATGGGCT TGTCTACTGG 
AAAACCGCTA CGGGTCGCTT CAAAGACATC CTGGCTCTGC TACTAACTGA CGTGCTGCTC 
TTCTTACAAG AAAAAGACCA GAAATACATC TTTGCAGCTG TTGACCAGAA GCCATCAGTG 
ATTTCCCTCC AGAAGCTGAT TGCTAGAGAA GTTGCCAACG AGGAGAGAGG CATGTTTCTG 
ATCAGTGCTT CGTCAGCCGG CCCCGAGATG TACGAAATCC ACACCAATTC CAAGGAGGAG 
CGGAATAACT GGATGAGGCG GATCCAGCAG GCAGTAGAAA GTTGTCCAGA GGAAGAAGGA 
GGAAGGACGA GCGAGTCGGA TGAAGAGAGG CGGAAAGCTG AAGCTAGAGT GGCCAAGATT 
CAGCAATGTC AAGAAATACT CAGTAACCAA GACCAACAGA TTTGCACGTA CTTGGAAGAA 
AAGCTGCATA TCTACGCTGA ACTTGGGGAA CTGAGTGGCT TTGAGGATGT CCACCTAGAA 
CCCCATCTGC TCATCAAACC TGACCCTGGA GAGCCTCCCC AGGCAGCGTC CCTGCTTGCA 
GCAGCCCTGA GAGAAGCCGA GAGTCTGCAA GTGGCTGTGA AGGCTTCAAA GATGGGCGAT 
GTAAGCCAGT CATCCGAAGA AAGCCCTGGA GGGACTGTCC TGATGGACAC ACCCAGTACA 
CAGGATGTAC CAGCGTCACC AACTGCTTCA TTAGTCACAG AAGGGACAGA AGGAAGAGGC 
TGTTGGGATG TGGATCCCGG GCTCCAGGGT GTGGTAACCG ACTTGGCAGT CTCTGATGCA 
GGGGAGAAGG TGGAATATAG AAGTTTCTCC GGTTCTTCAC AGTCAGAGAT TATACAAGCA 
ATACAGAATT TAACGCGTCT CCTCTACAGC CTTCAGGCTG CCTTGACCAT CCAGGACAGT 
CACATTGAGA TCCACAAACT GGTTCTACAG CAGCGGGAGA GCTTGGCCCC TAGCCACTCC 
TTCCGAGGTG GCCCCTTGCA AGACCAGGAG AAATCCCGCT ACCTGGAAAA GCAGCGTGAA 
GAGCTGGCCA ACATCCACAA GCTTCAACAC CAGTTCCAGC AGGAGCAGCG GCGCTGGCAC 
CGCACGTGCG ACCAGCAACA GCGCGAGCAG GAAGCCCAAG AGAGCTGGCT GCAGGCGCGG 
GAACGTGAGT GCCAGTCCCA GGAGGAATTG TTGCTGCGCC ACCGTAGCGA GCTGGACCAC 
CAACTCCAGG AGTACCAGCA GAGCCTTGAG CGGCTACGGG AGGGCCAGCG GATGGTGGAG 
AGGGAGAGAC AGAAGATGCG TGTCCAGCAG GGTCTGCTGG GCCACTGCAA GCATAGTCGC 
CAGCGGAGCC TCCCAGCCGT CTTCTCTCCA GGGAGCAAGG AGGTAACAGA ACTTAACCGA 
GCTGAGAGTT TGTGTCATGA AAATTCATTT TTCATCAATG AAGCTTTTGG ACACATGTCA 
CTTAACACGT CCAATAAACC AAACCCATCA GGGGTCCCCT GGGATGCTCA CCCCCTGGAG 
GGGTCTCATT TTGATTTGGC AAGGACTAGT GAGAGTCCAA CAGAACTGAA GATAGACATT 
TCTCAGCCTC CTGATGTCAA CTCTGAACTC TGGACAACGG GGCCGGGCCA TCAGAGACCT 
GCTCTCCAAG AAAACAGCAA GGAGTCTTAT AAAAATGTTG CAGATTTGGA CTCCTTCCAG 
AGTGAATCGT CATCTCCCCA GGACTCCAAT CAGCGTGGCC CCCAGCCACA GACACTCACA 
ACAGAAGCAA AACTAAGTCT TCCGATGGCG GCTGGACACG GTGGCGATGC TGGAGACGGG 
GCTGAAGAAA ACATTCTTTA CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030102988.1
CDS	352..4578
Translation	MPSGAAETEEEVRNLESGRSPSEEEEDAKSIKSQVDGPSEHEDQDRLPLDRSFDGLKKSKHVPASLAAGQLSDVLNGGDEVYANCMVIDQVGDLDINYINLEGLSTHTSPESGRSMLGPQACMHTLPPDTSPCGRPLIENSEGTLDAAASQSFVTPSSSRTSNLNLSFGLHGFEKEQSHLKKRSSSLDALVADSEGEGGSEPPICYAVGSQSSPRTGLPSGDELDSFETNTEPDCNISRTESLSLSSTLHSKESLLSGIRSRSYSCSSPKISSGKSRLVRDFTVCSTSEEQRSYSFQEPPGEKRIQEEEWDEYVIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKMKNKDTKEKEKMNRHQFVPGTFSGVLQCSGCDKTLLGKESLQCANCKANTHKGCKDAVPPCTKKFQEKYNKNKPQSILGSSSVRDVPAPGLSLHPSSSMPIGLPAGRKEFAAQVHPLSRSVPGTTLESFRRAVTSLESEGDSWRSRSHSDELFQSMGSSPSTESFMMEDVVDSSLWIDLSSDAQEFEAESWSLVVDPSFCSRQEKDVIKRQDVIFELMQTEVHHIQTLLIMSEVFRKGMKEELQLDHSTVDKIFPCLDELLETHRHFFFSMKERRQESCAGSDRNFVINQIGDILVQQFSEENASKMKRIYGEFCSHHKEAMSLFKELQQNKKFQNFIKIRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKERTEEHRDLCKALGLIKDMIAAVDLKVSEYEKNQKWLEILNKIENKTYTKLKNGHVFRKQALLSQERALLHDGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNNWMRRIQQAVESCPEEEGGRTSESDEERRKAEARVAKIQQCQEILSNQDQQICTYLEEKLHIYAELGELSGFEDVHLEPHLLIKPDPGEPPQAASLLAAALREAESLQVAVKASKMGDVSQSSEESPGGTVLMDTPSTQDVPASPTASLVTEGTEGRGCWDVDPGLQGVVTDLAVSDAGEKVEYRSFSGSSQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIHKLVLQQRESLAPSHSFRGGPLQDQEKSRYLEKQREELANIHKLQHQFQQEQRRWHRTCDQQQREQEAQESWLQARERECQSQEELLLRHRSELDHQLQEYQQSLERLREGQRMVERERQKMRVQQGLLGHCKHSRQRSLPAVFSPGSKEVTELNRAESLCHENSFFINEAFGHMSLNTSNKPNPSGVPWDAHPLEGSHFDLARTSESPTELKIDISQPPDVNSELWTTGPGHQRPALQENSKESYKNVADLDSFQSESSSPQDSNQRGPQPQTLTTEAKLSLPMAAGHGGDAGDGAEENILYL

Target ORF information:

RefSeq Version	XM_030247128.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030247128.1

ORF Insert Sequence:

ATGCCTTCTG GAGCTGCAGA AACTGAAGAA GAGGTTAGGA ATTTGGAGTC TGGCAGATCA 
CCATCGGAGG AGGAGGAGGA TGCAAAGAGC ATCAAAAGCC AGGTGGATGG GCCGAGTGAA 
CACGAAGACC AGGACCGCCT ACCTCTGGAT CGCTCCTTTG ATGGCCTCAA AAAATCCAAG 
CATGTCCCAG CGTCCCTTGC TGCAGGCCAG CTCTCTGATG TTCTGAACGG GGGTGATGAA 
GTCTACGCTA ACTGTATGGT GATTGACCAG GTTGGCGATT TGGATATCAA CTATATAAAC 
CTAGAGGGAC TCTCCACCCA TACGAGTCCC GAGTCTGGGA GGTCCATGCT GGGGCCTCAG 
GCTTGCATGC ACACCCTTCC TCCCGACACC AGTCCCTGCG GGCGCCCACT CATTGAGAAC 
AGTGAGGGGA CCCTGGATGC TGCAGCTTCG CAGTCTTTCG TGACACCATC CAGTTCTCGC 
ACTTCCAACT TGAACCTTTC TTTTGGTCTC CATGGATTTG AAAAGGAGCA GAGTCATCTC 
AAGAAAAGGA GTTCTAGCCT GGATGCCCTG GTTGCCGACA GTGAGGGGGA AGGAGGTTCT 
GAGCCCCCCA TCTGCTATGC TGTGGGCTCT CAGAGTTCCC CAAGAACTGG ACTCCCCAGC 
GGGGATGAAC TGGACTCTTT TGAGACCAAC ACTGAACCAG ACTGTAATAT CTCCAGGACC 
GAATCACTCT CTCTGTCCAG TACTCTGCAT TCAAAGGAGT CCTTGCTTTC TGGGATCCGG 
TCTCGCTCCT ACTCCTGCTC ATCACCCAAA ATCTCTTCAG GAAAATCCCG GCTAGTGCGC 
GACTTCACAG TGTGCAGTAC CAGCGAAGAA CAGAGATCAT ATAGTTTCCA GGAGCCACCA 
GGAGAAAAAA GGATTCAGGA AGAAGAATGG GATGAATACG TCATTCCTGC CAAATCCGAG 
TCTGAAAAGT ACAAAGTGAG CCGAACTTTC AGCTTCCTCA TGAACAGGAT GACCAGCCCA 
CGGAACAAAT CTAAGATGAA AAACAAGGAC ACTAAAGAAA AAGAAAAAAT GAATCGACAT 
CAGTTTGTCC CGGGAACGTT CTCCGGGGTT CTGCAGTGCT CCGGTTGTGA TAAAACACTC 
CTGGGGAAAG AGTCACTCCA GTGTGCCAAC TGTAAGGCAA ACACACACAA GGGCTGCAAA 
GATGCTGTGC CTCCGTGTAC CAAGAAATTC CAAGAGAAAT ACAACAAGAA CAAACCACAG 
AGCATCCTTG GAAGCTCTTC AGTCAGAGAC GTCCCAGCAC CTGGGCTCTC CTTGCACCCT 
TCTTCCTCTA TGCCCATCGG ACTGCCAGCT GGAAGGAAGG AATTTGCGGC TCAGGTGCAT 
CCACTGTCCA GAAGTGTCCC CGGCACCACT TTGGAAAGCT TCAGGAGGGC AGTGACATCG 
TTAGAGTCTG AGGGTGACAG CTGGAGAAGC AGGTCTCATT CCGATGAGCT GTTCCAGTCT 
ATGGGCTCTT CTCCTTCCAC GGAGTCATTC ATGATGGAAG ATGTTGTGGA TTCTTCTCTG 
TGGATCGACC TCAGTAGTGA TGCTCAGGAG TTTGAAGCAG AGTCTTGGAG TCTCGTGGTG 
GACCCTTCCT TTTGCAGTAG GCAGGAGAAG GATGTCATCA AAAGACAAGA TGTCATTTTT 
GAGCTGATGC AGACAGAGGT CCACCACATC CAGACTCTGC TCATCATGTC CGAGGTCTTC 
AGGAAAGGCA TGAAGGAGGA ACTGCAGCTG GACCACAGCA CGGTGGACAA GATCTTCCCC 
TGCTTAGATG AGCTGCTGGA GACTCACCGG CACTTCTTCT TCAGCATGAA GGAGCGCAGG 
CAGGAGTCCT GCGCTGGCAG TGACAGGAAC TTTGTGATCA ACCAAATTGG AGACATTCTG 
GTACAACAGT TTTCAGAGGA AAATGCAAGC AAAATGAAGA GGATCTATGG AGAATTCTGC 
AGCCATCACA AAGAAGCCAT GAGTCTCTTT AAAGAGCTGC AGCAGAACAA GAAGTTTCAG 
AATTTTATTA AGATCCGAAA TAGCAACCTG TTGGCTCGTC GCCGCGGAAT CCCGGAGTGC 
ATCCTGCTAG TTACCCAGCG CATCACAAAG TACCCAGTCT TGGTGGAAAG GATCCTGCAG 
TACACCAAGG AAAGGACTGA AGAGCACCGG GACCTGTGCA AAGCTCTCGG CTTAATTAAA 
GACATGATCG CAGCCGTGGA CCTGAAAGTC AGCGAATACG AGAAGAACCA AAAATGGCTC 
GAGATCCTAA ATAAAATCGA GAACAAAACA TACACAAAGC TCAAAAATGG CCACGTGTTT 
AGAAAGCAGG CGCTGCTGAG CCAGGAACGG GCTCTCCTGC ATGATGGGCT TGTCTACTGG 
AAAACCGCTA CGGGTCGCTT CAAAGACATC CTGGCTCTGC TACTAACTGA CGTGCTGCTC 
TTCTTACAAG AAAAAGACCA GAAATACATC TTTGCAGCTG TTGACCAGAA GCCATCAGTG 
ATTTCCCTCC AGAAGCTGAT TGCTAGAGAA GTTGCCAACG AGGAGAGAGG CATGTTTCTG 
ATCAGTGCTT CGTCAGCCGG CCCCGAGATG TACGAAATCC ACACCAATTC CAAGGAGGAG 
CGGAATAACT GGATGAGGCG GATCCAGCAG GCAGTAGAAA GTTGTCCAGA GGAAGAAGGA 
GGAAGGACGA GCGAGTCGGA TGAAGAGAGG CGGAAAGCTG AAGCTAGAGT GGCCAAGATT 
CAGCAATGTC AAGAAATACT CAGTAACCAA GACCAACAGA TTTGCACGTA CTTGGAAGAA 
AAGCTGCATA TCTACGCTGA ACTTGGGGAA CTGAGTGGCT TTGAGGATGT CCACCTAGAA 
CCCCATCTGC TCATCAAACC TGACCCTGGA GAGCCTCCCC AGGCAGCGTC CCTGCTTGCA 
GCAGCCCTGA GAGAAGCCGA GAGTCTGCAA GTGGCTGTGA AGGCTTCAAA GATGGGCGAT 
GTAAGCCAGT CATCCGAAGA AAGCCCTGGA GGGACTGTCC TGATGGACAC ACCCAGTACA 
CAGGATGTAC CAGCGTCACC AACTGCTTCA TTAGTCACAG AAGGGACAGA AGGAAGAGGC 
TGTTGGGATG TGGATCCCGG GCTCCAGGGT GTGGTAACCG ACTTGGCAGT CTCTGATGCA 
GGGGAGAAGG TGGAATATAG AAGTTTCTCC GGTTCTTCAC AGTCAGAGAT TATACAAGCA 
ATACAGAATT TAACGCGTCT CCTCTACAGC CTTCAGGCTG CCTTGACCAT CCAGGACAGT 
CACATTGAGA TCCACAAACT GGTTCTACAG CAGCGGGAGA GCTTGGCCCC TAGCCACTCC 
TTCCGAGGTG GCCCCTTGCA AGACCAGGAG AAATCCCGCT ACCTGGAAAA GCAGCGTGAA 
GAGCTGGCCA ACATCCACAA GCTTCAACAC CAGTTCCAGC AGGAGCAGCG GCGCTGGCAC 
CGCACGTGCG ACCAGCAACA GCGCGAGCAG GAAGCCCAAG AGAGCTGGCT GCAGGCGCGG 
GAACGTGAGT GCCAGTCCCA GGAGGAATTG TTGCTGCGCC ACCGTAGCGA GCTGGACCAC 
CAACTCCAGG AGTACCAGCA GAGCCTTGAG CGGCTACGGG AGGGCCAGCG GATGGTGGAG 
AGGGAGAGAC AGAAGATGCG TGTCCAGCAG GGTCTGCTGG GCCACTGCAA GCATAGTCGC 
CAGCGGAGCC TCCCAGCCGT CTTCTCTCCA GGGAGCAAGG AGGTAACAGA ACTTAACCGA 
GCTGAGAGTT TGTGTCATGA AAATTCATTT TTCATCAATG AAGCTTTTGG ACACATGTCA 
CTTAACACGT CCAATAAACC AAACCCATCA GGGGTCCCCT GGGATGCTCA CCCCCTGGAG 
GGGTCTCATT TTGATTTGGC AAGGACTAGT GAGAGTCCAA CAGAACTGAA GATAGACATT 
TCTCAGCCTC CTGATGTCAA CTCTGAACTC TGGACAACGG GGCCGGGCCA TCAGAGACCT 
GCTCTCCAAG AAAACAGCAA GGAGTCTTAT AAAAATGTTG CAGATTTGGA CTCCTTCCAG 
AGTGAATCGT CATCTCCCCA GGACTCCAAT CAGCGTGGCC CCCAGCCACA GACACTCACA 
ACAGAAGCAA AACTAAGTCT TCCGATGGCG GCTGGACACG GTGGCGATGC TGGAGACGGG 
GCTGAAGAAA ACATTCTTTA CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgef28 ( XM_006517522.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517585.1 Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X8, mRNA.

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CloneID	OMu48950
Clone ID Related Accession (Same CDS sequence)	XM_006517522.3
Accession Version	XM_006517522.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rho guanine nucleotide exchange factor 28 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006517522.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGATTG ACCAGGTTGG CGATTTGGAT ATCAACTATA TAAACCTAGA GGGACTCTCC 
ACCCATACGA GTCCCGAGTC TGGGAGGTCC ATGCTGGGGC CTCAGGCTTG CATGCACACC 
CTTCCTCCCG ACACCAGTCC CTGCGGGCGC CCACTCATTG AGAACAGTGA GGGGACCCTG 
GATGCTGCAG CTTCGCAGTC TTTCGTGACA CCATCCAGTT CTCGCACTTC CAACTTGAAC 
CTTTCTTTTG GTCTCCATGG ATTTGAAAAG GAGCAGAGTC ATCTCAAGAA AAGGAGTTCT 
AGCCTGGATG CCCTGGTTGC CGACAGTGAG GGGGAAGGAG GTTCTGAGCC CCCCATCTGC 
TATGCTGTGG GCTCTCAGAG TTCCCCAAGA ACTGGACTCC CCAGCGGGGA TGAACTGGAC 
TCTTTTGAGA CCAACACTGA ACCAGACTGT AATATCTCCA GGACCGAATC ACTCTCTCTG 
TCCAGTACTC TGCATTCAAA GGAGTCCTTG CTTTCTGGGA TCCGGTCTCG CTCCTACTCC 
TGCTCATCAC CCAAAATCTC TTCAGGAAAA TCCCGGCTAG TGCGCGACTT CACAGTGTGC 
AGTACCAGCG AAGAACAGAG ATCATATAGT TTCCAGGAGC CACCAGGAGA AAAAAGGATT 
CAGGAAGAAG AATGGGATGA ATACGTCATT CCTGCCAAAT CCGAGTCTGA AAAGTACAAA 
GTGAGCCGAA CTTTCAGCTT CCTCATGAAC AGGATGACCA GCCCACGGAA CAAATCTAAG 
ATGAAAAACA AGGACACTAA AGAAAAAGAA AAAATGAATC GACATCAGTT TGTCCCGGGA 
ACGTTCTCCG GGGTTCTGCA GTGCTCCGGT TGTGATAAAA CACTCCTGGG GAAAGAGTCA 
CTCCAGTGTG CCAACTGTAA GGCAAACACA CACAAGGGCT GCAAAGATGC TGTGCCTCCG 
TGTACCAAGA AATTCCAAGA GAAATACAAC AAGAACAAAC CACAGAGCAT CCTTGGAAGC 
TCTTCAGTCA GAGACGTCCC AGCACCTGGG CTCTCCTTGC ACCCTTCTTC CTCTATGCCC 
ATCGGACTGC CAGCTGGAAG GAAGGAATTT GCGGCTCAGG TGCATCCACT GTCCAGAAGT 
GTCCCCGGCA CCACTTTGGA AAGCTTCAGG AGGGCAGTGA CATCGTTAGA GTCTGAGGGT 
GACAGCTGGA GAAGCAGGTC TCATTCCGAT GAGCTGTTCC AGTCTATGGG CTCTTCTCCT 
TCCACGGAGT CATTCATGAT GGAAGATGTT GTGGATTCTT CTCTGTGGAT CGACCTCAGT 
AGTGATGCTC AGGAGTTTGA AGCAGAGTCT TGGAGTCTCG TGGTGGACCC TTCCTTTTGC 
AGTAGGCAGG AGAAGGATGT CATCAAAAGA CAAGATGTCA TTTTTGAGCT GATGCAGACA 
GAGGTCCACC ACATCCAGAC TCTGCTCATC ATGTCCGAGG TCTTCAGGAA AGGCATGAAG 
GAGGAACTGC AGCTGGACCA CAGCACGGTG GACAAGATCT TCCCCTGCTT AGATGAGCTG 
CTGGAGACTC ACCGGCACTT CTTCTTCAGC ATGAAGGAGC GCAGGCAGGA GTCCTGCGCT 
GGCAGTGACA GGAACTTTGT GATCAACCAA ATTGGAGACA TTCTGGTACA ACAGTTTTCA 
GAGGAAAATG CAAGCAAAAT GAAGAGGATC TATGGAGAAT TCTGCAGCCA TCACAAAGAA 
GCCATGAGTC TCTTTAAAGA GCTGCAGCAG AACAAGAAGT TTCAGAATTT TATTAAGATC 
CGAAATAGCA ACCTGTTGGC TCGTCGCCGC GGAATCCCGG AGTGCATCCT GCTAGTTACC 
CAGCGCATCA CAAAGTACCC AGTCTTGGTG GAAAGGATCC TGCAGTACAC CAAGGAAAGG 
ACTGAAGAGC ACCGGGACCT GTGCAAAGCT CTCGGCTTAA TTAAAGACAT GATCGCAGCC 
GTGGACCTGA AAGTCAGCGA ATACGAGAAG AACCAAAAAT GGCTCGAGAT CCTAAATAAA 
ATCGAGAACA AAACATACAC AAAGCTCAAA AATGGCCACG TGTTTAGAAA GCAGGCGCTG 
CTGAGCCAGG AACGGGCTCT CCTGCATGAT GGGCTTGTCT ACTGGAAAAC CGCTACGGGT 
CGCTTCAAAG ACATCCTGGC TCTGCTACTA ACTGACGTGC TGCTCTTCTT ACAAGAAAAA 
GACCAGAAAT ACATCTTTGC AGCTGTTGAC CAGAAGCCAT CAGTGATTTC CCTCCAGAAG 
CTGATTGCTA GAGAAGTTGC CAACGAGGAG AGAGGCATGT TTCTGATCAG TGCTTCGTCA 
GCCGGCCCCG AGATGTACGA AATCCACACC AATTCCAAGG AGGAGCGGAA TAACTGGATG 
AGGCGGATCC AGCAGGCAGT AGAAAGTTGT CCAGAGGAAG AAGGAGGAAG GACGAGCGAG 
TCGGATGAAG AGAGGCGGAA AGCTGAAGCT AGAGTGGCCA AGATTCAGCA ATGTCAAGAA 
ATACTCAGTA ACCAAGACCA ACAGATTTGC ACGTACTTGG AAGAAAAGCT GCATATCTAC 
GCTGAACTTG GGGAACTGAG TGGCTTTGAG GATGTCCACC TAGAACCCCA TCTGCTCATC 
AAACCTGACC CTGGAGAGCC TCCCCAGGCA GCGTCCCTGC TTGCAGCAGC CCTGAGAGAA 
GCCGAGAGTC TGCAAGTGGC TGTGAAGGCT TCAAAGATGG GCGATGTAAG CCAGTCATCC 
GAAGAAAGCC CTGGAGGGAC TGTCCTGATG GACACACCCA GTACACAGGA TGTACCAGCG 
TCACCAACTG CTTCATTAGT CACAGAAGGG ACAGAAGGAA GAGGCTGTTG GGATGTGGAT 
CCCGGGCTCC AGGGTGTGGT AACCGACTTG GCAGTCTCTG ATGCAGGGGA GAAGGTGGAA 
TATAGAAGTT TCTCCGGTTC TTCACAGTCA GAGATTATAC AAGCAATACA GAATTTAACG 
CGTCTCCTCT ACAGCCTTCA GGCTGCCTTG ACCATCCAGG ACAGTCACAT TGAGATCCAC 
AAACTGGTTC TACAGCAGCG GGAGAGCTTG GCCCCTAGCC ACTCCTTCCG AGGTGGCCCC 
TTGCAAGACC AGGAGAAATC CCGCTACCTG GAAAAGCAGC GTGAAGAGCT GGCCAACATC 
CACAAGCTTC AACACCAGTT CCAGCAGGAG CAGCGGCGCT GGCACCGCAC GTGCGACCAG 
CAACAGCGCG AGCAGGAAGC CCAAGAGAGC TGGCTGCAGG CGCGGGAACG TGAGTGCCAG 
TCCCAGGAGG AATTGTTGCT GCGCCACCGT AGCGAGCTGG ACCACCAACT CCAGGAGTAC 
CAGCAGAGCC TTGAGCGGCT ACGGGAGGGC CAGCGGATGG TGGAGAGGGA GAGACAGAAG 
ATGCGTGTCC AGCAGGGTCT GCTGGGCCAC TGCAAGCATA GTCGCCAGCG GAGCCTCCCA 
GCCGTCTTCT CTCCAGGGAG CAAGGAGGTA ACAGAACTTA ACCGAGCTGA GAGTTTGTGT 
CATGAAAATT CATTTTTCAT CAATGAAGCT TTTGGACACA TGTCACTTAA CACGTCCAAT 
AAACCAAACC CATCAGGGGT CCCCTGGGAT GCTCACCCCC TGGAGGGGTC TCATTTTGAT 
TTGGCAAGGA CTAGTGAGAG TCCAACAGAA CTGAAGATAG ACATTTCTCA GCCTCCTGAT 
GTCAACTCTG AACTCTGGAC AACGGGGCCG GGCCATCAGA GACCTGCTCT CCAAGAAAAC 
AGCAAGGAGT CTTATAAAAA TGTTGCAGAT TTGGACTCCT TCCAGAGTGA ATCGTCATCT 
CCCCAGGACT CCAATCAGCG TGGCCCCCAG CCACAGACAC TCACAACAGA AGCAAAACTA 
AGTCTTCCGA TGGCGGCTGG ACACGGTGGC GATGCTGGAG ACGGGGCTGA AGAAAACATT 
CTTTACCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517585.1
CDS	322..4293
Translation	MVIDQVGDLDINYINLEGLSTHTSPESGRSMLGPQACMHTLPPDTSPCGRPLIENSEGTLDAAASQSFVTPSSSRTSNLNLSFGLHGFEKEQSHLKKRSSSLDALVADSEGEGGSEPPICYAVGSQSSPRTGLPSGDELDSFETNTEPDCNISRTESLSLSSTLHSKESLLSGIRSRSYSCSSPKISSGKSRLVRDFTVCSTSEEQRSYSFQEPPGEKRIQEEEWDEYVIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKMKNKDTKEKEKMNRHQFVPGTFSGVLQCSGCDKTLLGKESLQCANCKANTHKGCKDAVPPCTKKFQEKYNKNKPQSILGSSSVRDVPAPGLSLHPSSSMPIGLPAGRKEFAAQVHPLSRSVPGTTLESFRRAVTSLESEGDSWRSRSHSDELFQSMGSSPSTESFMMEDVVDSSLWIDLSSDAQEFEAESWSLVVDPSFCSRQEKDVIKRQDVIFELMQTEVHHIQTLLIMSEVFRKGMKEELQLDHSTVDKIFPCLDELLETHRHFFFSMKERRQESCAGSDRNFVINQIGDILVQQFSEENASKMKRIYGEFCSHHKEAMSLFKELQQNKKFQNFIKIRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKERTEEHRDLCKALGLIKDMIAAVDLKVSEYEKNQKWLEILNKIENKTYTKLKNGHVFRKQALLSQERALLHDGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNNWMRRIQQAVESCPEEEGGRTSESDEERRKAEARVAKIQQCQEILSNQDQQICTYLEEKLHIYAELGELSGFEDVHLEPHLLIKPDPGEPPQAASLLAAALREAESLQVAVKASKMGDVSQSSEESPGGTVLMDTPSTQDVPASPTASLVTEGTEGRGCWDVDPGLQGVVTDLAVSDAGEKVEYRSFSGSSQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIHKLVLQQRESLAPSHSFRGGPLQDQEKSRYLEKQREELANIHKLQHQFQQEQRRWHRTCDQQQREQEAQESWLQARERECQSQEELLLRHRSELDHQLQEYQQSLERLREGQRMVERERQKMRVQQGLLGHCKHSRQRSLPAVFSPGSKEVTELNRAESLCHENSFFINEAFGHMSLNTSNKPNPSGVPWDAHPLEGSHFDLARTSESPTELKIDISQPPDVNSELWTTGPGHQRPALQENSKESYKNVADLDSFQSESSSPQDSNQRGPQPQTLTTEAKLSLPMAAGHGGDAGDGAEENILYL

Target ORF information:

RefSeq Version	XM_006517522.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006517522.3

ORF Insert Sequence:

ATGGTGATTG ACCAGGTTGG CGATTTGGAT ATCAACTATA TAAACCTAGA GGGACTCTCC 
ACCCATACGA GTCCCGAGTC TGGGAGGTCC ATGCTGGGGC CTCAGGCTTG CATGCACACC 
CTTCCTCCCG ACACCAGTCC CTGCGGGCGC CCACTCATTG AGAACAGTGA GGGGACCCTG 
GATGCTGCAG CTTCGCAGTC TTTCGTGACA CCATCCAGTT CTCGCACTTC CAACTTGAAC 
CTTTCTTTTG GTCTCCATGG ATTTGAAAAG GAGCAGAGTC ATCTCAAGAA AAGGAGTTCT 
AGCCTGGATG CCCTGGTTGC CGACAGTGAG GGGGAAGGAG GTTCTGAGCC CCCCATCTGC 
TATGCTGTGG GCTCTCAGAG TTCCCCAAGA ACTGGACTCC CCAGCGGGGA TGAACTGGAC 
TCTTTTGAGA CCAACACTGA ACCAGACTGT AATATCTCCA GGACCGAATC ACTCTCTCTG 
TCCAGTACTC TGCATTCAAA GGAGTCCTTG CTTTCTGGGA TCCGGTCTCG CTCCTACTCC 
TGCTCATCAC CCAAAATCTC TTCAGGAAAA TCCCGGCTAG TGCGCGACTT CACAGTGTGC 
AGTACCAGCG AAGAACAGAG ATCATATAGT TTCCAGGAGC CACCAGGAGA AAAAAGGATT 
CAGGAAGAAG AATGGGATGA ATACGTCATT CCTGCCAAAT CCGAGTCTGA AAAGTACAAA 
GTGAGCCGAA CTTTCAGCTT CCTCATGAAC AGGATGACCA GCCCACGGAA CAAATCTAAG 
ATGAAAAACA AGGACACTAA AGAAAAAGAA AAAATGAATC GACATCAGTT TGTCCCGGGA 
ACGTTCTCCG GGGTTCTGCA GTGCTCCGGT TGTGATAAAA CACTCCTGGG GAAAGAGTCA 
CTCCAGTGTG CCAACTGTAA GGCAAACACA CACAAGGGCT GCAAAGATGC TGTGCCTCCG 
TGTACCAAGA AATTCCAAGA GAAATACAAC AAGAACAAAC CACAGAGCAT CCTTGGAAGC 
TCTTCAGTCA GAGACGTCCC AGCACCTGGG CTCTCCTTGC ACCCTTCTTC CTCTATGCCC 
ATCGGACTGC CAGCTGGAAG GAAGGAATTT GCGGCTCAGG TGCATCCACT GTCCAGAAGT 
GTCCCCGGCA CCACTTTGGA AAGCTTCAGG AGGGCAGTGA CATCGTTAGA GTCTGAGGGT 
GACAGCTGGA GAAGCAGGTC TCATTCCGAT GAGCTGTTCC AGTCTATGGG CTCTTCTCCT 
TCCACGGAGT CATTCATGAT GGAAGATGTT GTGGATTCTT CTCTGTGGAT CGACCTCAGT 
AGTGATGCTC AGGAGTTTGA AGCAGAGTCT TGGAGTCTCG TGGTGGACCC TTCCTTTTGC 
AGTAGGCAGG AGAAGGATGT CATCAAAAGA CAAGATGTCA TTTTTGAGCT GATGCAGACA 
GAGGTCCACC ACATCCAGAC TCTGCTCATC ATGTCCGAGG TCTTCAGGAA AGGCATGAAG 
GAGGAACTGC AGCTGGACCA CAGCACGGTG GACAAGATCT TCCCCTGCTT AGATGAGCTG 
CTGGAGACTC ACCGGCACTT CTTCTTCAGC ATGAAGGAGC GCAGGCAGGA GTCCTGCGCT 
GGCAGTGACA GGAACTTTGT GATCAACCAA ATTGGAGACA TTCTGGTACA ACAGTTTTCA 
GAGGAAAATG CAAGCAAAAT GAAGAGGATC TATGGAGAAT TCTGCAGCCA TCACAAAGAA 
GCCATGAGTC TCTTTAAAGA GCTGCAGCAG AACAAGAAGT TTCAGAATTT TATTAAGATC 
CGAAATAGCA ACCTGTTGGC TCGTCGCCGC GGAATCCCGG AGTGCATCCT GCTAGTTACC 
CAGCGCATCA CAAAGTACCC AGTCTTGGTG GAAAGGATCC TGCAGTACAC CAAGGAAAGG 
ACTGAAGAGC ACCGGGACCT GTGCAAAGCT CTCGGCTTAA TTAAAGACAT GATCGCAGCC 
GTGGACCTGA AAGTCAGCGA ATACGAGAAG AACCAAAAAT GGCTCGAGAT CCTAAATAAA 
ATCGAGAACA AAACATACAC AAAGCTCAAA AATGGCCACG TGTTTAGAAA GCAGGCGCTG 
CTGAGCCAGG AACGGGCTCT CCTGCATGAT GGGCTTGTCT ACTGGAAAAC CGCTACGGGT 
CGCTTCAAAG ACATCCTGGC TCTGCTACTA ACTGACGTGC TGCTCTTCTT ACAAGAAAAA 
GACCAGAAAT ACATCTTTGC AGCTGTTGAC CAGAAGCCAT CAGTGATTTC CCTCCAGAAG 
CTGATTGCTA GAGAAGTTGC CAACGAGGAG AGAGGCATGT TTCTGATCAG TGCTTCGTCA 
GCCGGCCCCG AGATGTACGA AATCCACACC AATTCCAAGG AGGAGCGGAA TAACTGGATG 
AGGCGGATCC AGCAGGCAGT AGAAAGTTGT CCAGAGGAAG AAGGAGGAAG GACGAGCGAG 
TCGGATGAAG AGAGGCGGAA AGCTGAAGCT AGAGTGGCCA AGATTCAGCA ATGTCAAGAA 
ATACTCAGTA ACCAAGACCA ACAGATTTGC ACGTACTTGG AAGAAAAGCT GCATATCTAC 
GCTGAACTTG GGGAACTGAG TGGCTTTGAG GATGTCCACC TAGAACCCCA TCTGCTCATC 
AAACCTGACC CTGGAGAGCC TCCCCAGGCA GCGTCCCTGC TTGCAGCAGC CCTGAGAGAA 
GCCGAGAGTC TGCAAGTGGC TGTGAAGGCT TCAAAGATGG GCGATGTAAG CCAGTCATCC 
GAAGAAAGCC CTGGAGGGAC TGTCCTGATG GACACACCCA GTACACAGGA TGTACCAGCG 
TCACCAACTG CTTCATTAGT CACAGAAGGG ACAGAAGGAA GAGGCTGTTG GGATGTGGAT 
CCCGGGCTCC AGGGTGTGGT AACCGACTTG GCAGTCTCTG ATGCAGGGGA GAAGGTGGAA 
TATAGAAGTT TCTCCGGTTC TTCACAGTCA GAGATTATAC AAGCAATACA GAATTTAACG 
CGTCTCCTCT ACAGCCTTCA GGCTGCCTTG ACCATCCAGG ACAGTCACAT TGAGATCCAC 
AAACTGGTTC TACAGCAGCG GGAGAGCTTG GCCCCTAGCC ACTCCTTCCG AGGTGGCCCC 
TTGCAAGACC AGGAGAAATC CCGCTACCTG GAAAAGCAGC GTGAAGAGCT GGCCAACATC 
CACAAGCTTC AACACCAGTT CCAGCAGGAG CAGCGGCGCT GGCACCGCAC GTGCGACCAG 
CAACAGCGCG AGCAGGAAGC CCAAGAGAGC TGGCTGCAGG CGCGGGAACG TGAGTGCCAG 
TCCCAGGAGG AATTGTTGCT GCGCCACCGT AGCGAGCTGG ACCACCAACT CCAGGAGTAC 
CAGCAGAGCC TTGAGCGGCT ACGGGAGGGC CAGCGGATGG TGGAGAGGGA GAGACAGAAG 
ATGCGTGTCC AGCAGGGTCT GCTGGGCCAC TGCAAGCATA GTCGCCAGCG GAGCCTCCCA 
GCCGTCTTCT CTCCAGGGAG CAAGGAGGTA ACAGAACTTA ACCGAGCTGA GAGTTTGTGT 
CATGAAAATT CATTTTTCAT CAATGAAGCT TTTGGACACA TGTCACTTAA CACGTCCAAT 
AAACCAAACC CATCAGGGGT CCCCTGGGAT GCTCACCCCC TGGAGGGGTC TCATTTTGAT 
TTGGCAAGGA CTAGTGAGAG TCCAACAGAA CTGAAGATAG ACATTTCTCA GCCTCCTGAT 
GTCAACTCTG AACTCTGGAC AACGGGGCCG GGCCATCAGA GACCTGCTCT CCAAGAAAAC 
AGCAAGGAGT CTTATAAAAA TGTTGCAGAT TTGGACTCCT TCCAGAGTGA ATCGTCATCT 
CCCCAGGACT CCAATCAGCG TGGCCCCCAG CCACAGACAC TCACAACAGA AGCAAAACTA 
AGTCTTCCGA TGGCGGCTGG ACACGGTGGC GATGCTGGAG ACGGGGCTGA AGAAAACATT 
CTTTACCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgef28 ( XM_006517518.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517581.1 Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X1, mRNA.

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CloneID	OMu48946
Clone ID Related Accession (Same CDS sequence)	XM_006517518.2 , XM_006517518.3
Accession Version	XM_006517518.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rho guanine nucleotide exchange factor 28 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006517518.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCGGC GACGAGCCCG GCTGGCCAGA GATGCAGGAG CCATGGAGTT GAGCTGCAGT 
GAAGTGCCTC TTTATGGACA GAAGACGGTC TATGCAAAGT TTGGCAAAAA CGTGTATCTA 
CCCGAAGATG CGGAGTTTTA CTTTGTTTAT GGCGGGTCGC ATCAGAGGCA CGTGGTGATT 
GCTGACCGTG TCCAGGACAA TGTCCTCCAA TCTAGCATTC CAGGCCACTG GCTTCAGGAG 
ACGGTGACGG TATCTGTGTG CCTCTGCTCG GAAGGGTACT CCCCAGTGAC CATGGGCTCT 
GGCTCAGTGA CCTACGTGGA CAACATGGCC TGCAGGCTGG CTCGTCTGCT GGTCACTCAG 
GCTGACCGAC TCACGGCCTG CAGTCACCAG ACGCTGCTGA CCCCGTTTGC CCTGACAGTG 
GAAGCGCTGC CTGCCTTGGA TGAGGAGCTC GTGCTGGCTC TGACCCAACT GGAGCTGCCT 
TTGGGTTGGA CAGTCCTGGG AAATTCCTCA CTTGAAGTAT CTCTACACAG AGAGTCTCTC 
CTGCATCTGG CTGTGAGATG GGCCCTCCCT AAGCTATTCC ACTTCCTCTT GTGTCTCCCT 
GGAGGAGTCA AGGCCTTGAA GCTACCTAAT GAAGAGGCCA CCACACCATT AGACTTAGCT 
TTGCAAGGTG GACACTCCAC ACTGGTAGAA GACATCACAA ATTTTCAGGG TAGCCATTCC 
CCAGGCTTCT CCCGACTGCG TCTTAACGAA GAGGCCACTC TGCAGTTTGT TCATTCATCG 
GAAACATTGA CCCTGACCGT TAACCACACA GCTGAGCATC TCTTGGAAGC AGACATCAAA 
CTCTTCCGGA AATATTTCTG GGACAGAGCC TTTCTTGTCA AGGCTCTTGA GCAAGAAGCC 
AAGACTGAGA AAGCAACCAT GCCTTCTGGA GCTGCAGAAA CTGAAGAAGA GGTTAGGAAT 
TTGGAGTCTG GCAGATCACC ATCGGAGGAG GAGGAGGATG CAAAGAGCAT CAAAAGCCAG 
GTGGATGGGC CGAGTGAACA CGAAGACCAG GACCGCCTAC CTCTGGATCG CTCCTTTGAT 
GGCCTCAAAA AATCCAAGCA TGTCCCAGCG TCCCTTGCTG CAGGCCAGCT CTCTGATGTT 
CTGAACGGGG GTGATGAAGT CTACGCTAAC TGTATGGTGA TTGACCAGGT TGGCGATTTG 
GATATCAACT ATATAAACCT AGAGGGACTC TCCACCCATA CGAGTCCCGA GTCTGGGAGG 
TCCATGCTGG GGCCTCAGGC TTGCATGCAC ACCCTTCCTC CCGACACCAG TCCCTGCGGG 
CGCCCACTCA TTGAGAACAG TGAGGGGACC CTGGATGCTG CAGCTTCGCA GTCTTTCGTG 
ACACCATCCA GTTCTCGCAC TTCCAACTTG AACCTTTCTT TTGGTCTCCA TGGATTTGAA 
AAGGAGCAGA GTCATCTCAA GAAAAGGAGT TCTAGCCTGG ATGCCCTGGT TGCCGACAGT 
GAGGGGGAAG GAGGTTCTGA GCCCCCCATC TGCTATGCTG TGGGCTCTCA GAGTTCCCCA 
AGAACTGGAC TCCCCAGCGG GGATGAACTG GACTCTTTTG AGACCAACAC TGAACCAGAC 
TGTAATATCT CCAGGACCGA ATCACTCTCT CTGTCCAGTA CTCTGCATTC AAAGGAGTCC 
TTGCTTTCTG GGATCCGGTC TCGCTCCTAC TCCTGCTCAT CACCCAAAAT CTCTTCAGGA 
AAATCCCGGC TAGTGCGCGA CTTCACAGTG TGCAGTACCA GCGAAGAACA GAGATCATAT 
AGTTTCCAGG AGCCACCAGG AGAAAAAAGG ATTCAGGAAG AAGAATGGGA TGAATACGTC 
ATTCCTGCCA AATCCGAGTC TGAAAAGTAC AAAGTGAGCC GAACTTTCAG CTTCCTCATG 
AACAGGATGA CCAGCCCACG GAACAAATCT AAGATGAAAA ACAAGGACAC TAAAGAAAAA 
GAAAAAATGA ATCGACATCA GTTTGTCCCG GGAACGTTCT CCGGGGTTCT GCAGTGCTCC 
GGTTGTGATA AAACACTCCT GGGGAAAGAG TCACTCCAGT GTGCCAACTG TAAGGCAAAC 
ACACACAAGG GCTGCAAAGA TGCTGTGCCT CCGTGTACCA AGAAATTCCA AGAGAAATAC 
AACAAGAACA AACCACAGAG CATCCTTGGA AGCTCTTCAG TCAGAGACGT CCCAGCACCT 
GGGCTCTCCT TGCACCCTTC TTCCTCTATG CCCATCGGAC TGCCAGCTGG AAGGAAGGAA 
TTTGCGGCTC AGGTGCATCC ACTGTCCAGA AGTGTCCCCG GCACCACTTT GGAAAGCTTC 
AGGAGGGCAG TGACATCGTT AGAGTCTGAG GGTGACAGCT GGAGAAGCAG GTCTCATTCC 
GATGAGCTGT TCCAGTCTAT GGGCTCTTCT CCTTCCACGG AGTCATTCAT GATGGAAGAT 
GTTGTGGATT CTTCTCTGTG GATCGACCTC AGTAGTGATG CTCAGGAGTT TGAAGCAGAG 
TCTTGGAGTC TCGTGGTGGA CCCTTCCTTT TGCAGTAGGC AGGAGAAGGA TGTCATCAAA 
AGACAAGATG TCATTTTTGA GCTGATGCAG ACAGAGGTCC ACCACATCCA GACTCTGCTC 
ATCATGTCCG AGGTCTTCAG GAAAGGCATG AAGGAGGAAC TGCAGCTGGA CCACAGCACG 
GTGGACAAGA TCTTCCCCTG CTTAGATGAG CTGCTGGAGA CTCACCGGCA CTTCTTCTTC 
AGCATGAAGG AGCGCAGGCA GGAGTCCTGC GCTGGCAGTG ACAGGAACTT TGTGATCAAC 
CAAATTGGAG ACATTCTGGT ACAACAGTTT TCAGAGGAAA ATGCAAGCAA AATGAAGAGG 
ATCTATGGAG AATTCTGCAG CCATCACAAA GAAGCCATGA GTCTCTTTAA AGAGCTGCAG 
CAGAACAAGA AGTTTCAGAA TTTTATTAAG ATCCGAAATA GCAACCTGTT GGCTCGTCGC 
CGCGGAATCC CGGAGTGCAT CCTGCTAGTT ACCCAGCGCA TCACAAAGTA CCCAGTCTTG 
GTGGAAAGGA TCCTGCAGTA CACCAAGGAA AGGACTGAAG AGCACCGGGA CCTGTGCAAA 
GCTCTCGGCT TAATTAAAGA CATGATCGCA GCCGTGGACC TGAAAGTCAG CGAATACGAG 
AAGAACCAAA AATGGCTCGA GATCCTAAAT AAAATCGAGA ACAAAACATA CACAAAGCTC 
AAAAATGGCC ACGTGTTTAG AAAGCAGGCG CTGCTGAGCC AGGAACGGGC TCTCCTGCAT 
GATGGGCTTG TCTACTGGAA AACCGCTACG GGTCGCTTCA AAGACATCCT GGCTCTGCTA 
CTAACTGACG TGCTGCTCTT CTTACAAGAA AAAGACCAGA AATACATCTT TGCAGCTGTT 
GACCAGAAGC CATCAGTGAT TTCCCTCCAG AAGCTGATTG CTAGAGAAGT TGCCAACGAG 
GAGAGAGGCA TGTTTCTGAT CAGTGCTTCG TCAGCCGGCC CCGAGATGTA CGAAATCCAC 
ACCAATTCCA AGGAGGAGCG GAATAACTGG ATGAGGCGGA TCCAGCAGGC AGTAGAAAGT 
TGTCCAGAGG AAGAAGGAGG AAGGACGAGC GAGTCGGATG AAGAGAGGCG GAAAGCTGAA 
GCTAGAGTGG CCAAGATTCA GCAATGTCAA GAAATACTCA GTAACCAAGA CCAACAGATT 
TGCACGTACT TGGAAGAAAA GCTGCATATC TACGCTGAAC TTGGGGAACT GAGTGGCTTT 
GAGGATGTCC ACCTAGAACC CCATCTGCTC ATCAAACCTG ACCCTGGAGA GCCTCCCCAG 
GCAGCGTCCC TGCTTGCAGC AGCCCTGAGA GAAGCCGAGA GTCTGCAAGT GGCTGTGAAG 
GCTTCAAAGA TGGGCGATGT AAGCCAGTCA TCCGAAGAAA GCCCTGGAGG GACTGTCCTG 
ATGGACACAC CCAGTACACA GGATGTACCA GCGTCACCAA CTGCTTCATT AGTCACAGAA 
GGGACAGAAG GAAGAGGCTG TTGGGATGTG GATCCCGGGC TCCAGGGTGT GGTAACCGAC 
TTGGCAGTCT CTGATGCAGG GGAGAAGGTG GAATATAGAA GTTTCTCCGG TTCTTCACAG 
TCAGAGATTA TACAAGCAAT ACAGAATTTA ACGCGTCTCC TCTACAGCCT TCAGGCTGCC 
TTGACCATCC AGGACAGTCA CATTGAGATC CACAAACTGG TTCTACAGCA GCGGGAGAGC 
TTGGCCCCTA GCCACTCCTT CCGAGGTGGC CCCTTGCAAG ACCAGGAGAA ATCCCGCTAC 
CTGGAAAAGC AGCGTGAAGA GCTGGCCAAC ATCCACAAGC TTCAACACCA GTTCCAGCAG 
GAGCAGCGGC GCTGGCACCG CACGTGCGAC CAGCAACAGC GCGAGCAGGA AGCCCAAGAG 
AGCTGGCTGC AGGCGCGGGA ACGTGAGTGC CAGTCCCAGG AGGAATTGTT GCTGCGCCAC 
CGTAGCGAGC TGGACCACCA ACTCCAGGAG TACCAGCAGA GCCTTGAGCG GCTACGGGAG 
GGCCAGCGGA TGGTGGAGAG GGAGAGACAG AAGATGCGTG TCCAGCAGGG TCTGCTGGGC 
CACTGCAAGC ATAGTCGCCA GCGGAGCCTC CCAGCCGTCT TCTCTCCAGG GAGCAAGGAG 
GTAACAGAAC TTAACCGAGC TGAGAGTTTG TGTCATGAAA ATTCATTTTT CATCAATGAA 
GCTTTTGGAC ACATGTCACT TAACACGTCC AATAAACCAA ACCCATCAGG GGTCCCCTGG 
GATGCTCACC CCCTGGAGGG GTCTCATTTT GATTTGGCAA GGACTAGTGA GAGTCCAACA 
GAACTGAAGA TAGACATTTC TCAGCCTCCT GATGTCAACT CTGAACTCTG GACAACGGGG 
CCGGGCCATC AGAGACCTGC TCTCCAAGAA AACAGCAAGG AGTCTTATAA AAATGTTGCA 
GATTTGGACT CCTTCCAGAG TGAATCGTCA TCTCCCCAGG ACTCCAATCA GCGTGGCCCC 
CAGCCACAGA CACTCACAAC AGAAGCAAAA CTAAGTCTTC CGATGGCGGC TGGACACGGT 
GGCGATGCTG GAGACGGGGC TGAAGAAAAC ATTCTTTACC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517581.1
CDS	322..5466
Translation	MRRRRARLARDAGAMELSCSEVPLYGQKTVYAKFGKNVYLPEDAEFYFVYGGSHQRHVVIADRVQDNVLQSSIPGHWLQETVTVSVCLCSEGYSPVTMGSGSVTYVDNMACRLARLLVTQADRLTACSHQTLLTPFALTVEALPALDEELVLALTQLELPLGWTVLGNSSLEVSLHRESLLHLAVRWALPKLFHFLLCLPGGVKALKLPNEEATTPLDLALQGGHSTLVEDITNFQGSHSPGFSRLRLNEEATLQFVHSSETLTLTVNHTAEHLLEADIKLFRKYFWDRAFLVKALEQEAKTEKATMPSGAAETEEEVRNLESGRSPSEEEEDAKSIKSQVDGPSEHEDQDRLPLDRSFDGLKKSKHVPASLAAGQLSDVLNGGDEVYANCMVIDQVGDLDINYINLEGLSTHTSPESGRSMLGPQACMHTLPPDTSPCGRPLIENSEGTLDAAASQSFVTPSSSRTSNLNLSFGLHGFEKEQSHLKKRSSSLDALVADSEGEGGSEPPICYAVGSQSSPRTGLPSGDELDSFETNTEPDCNISRTESLSLSSTLHSKESLLSGIRSRSYSCSSPKISSGKSRLVRDFTVCSTSEEQRSYSFQEPPGEKRIQEEEWDEYVIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKMKNKDTKEKEKMNRHQFVPGTFSGVLQCSGCDKTLLGKESLQCANCKANTHKGCKDAVPPCTKKFQEKYNKNKPQSILGSSSVRDVPAPGLSLHPSSSMPIGLPAGRKEFAAQVHPLSRSVPGTTLESFRRAVTSLESEGDSWRSRSHSDELFQSMGSSPSTESFMMEDVVDSSLWIDLSSDAQEFEAESWSLVVDPSFCSRQEKDVIKRQDVIFELMQTEVHHIQTLLIMSEVFRKGMKEELQLDHSTVDKIFPCLDELLETHRHFFFSMKERRQESCAGSDRNFVINQIGDILVQQFSEENASKMKRIYGEFCSHHKEAMSLFKELQQNKKFQNFIKIRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKERTEEHRDLCKALGLIKDMIAAVDLKVSEYEKNQKWLEILNKIENKTYTKLKNGHVFRKQALLSQERALLHDGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNNWMRRIQQAVESCPEEEGGRTSESDEERRKAEARVAKIQQCQEILSNQDQQICTYLEEKLHIYAELGELSGFEDVHLEPHLLIKPDPGEPPQAASLLAAALREAESLQVAVKASKMGDVSQSSEESPGGTVLMDTPSTQDVPASPTASLVTEGTEGRGCWDVDPGLQGVVTDLAVSDAGEKVEYRSFSGSSQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIHKLVLQQRESLAPSHSFRGGPLQDQEKSRYLEKQREELANIHKLQHQFQQEQRRWHRTCDQQQREQEAQESWLQARERECQSQEELLLRHRSELDHQLQEYQQSLERLREGQRMVERERQKMRVQQGLLGHCKHSRQRSLPAVFSPGSKEVTELNRAESLCHENSFFINEAFGHMSLNTSNKPNPSGVPWDAHPLEGSHFDLARTSESPTELKIDISQPPDVNSELWTTGPGHQRPALQENSKESYKNVADLDSFQSESSSPQDSNQRGPQPQTLTTEAKLSLPMAAGHGGDAGDGAEENILYL

Target ORF information:

RefSeq Version	XM_006517518.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006517518.3

ORF Insert Sequence:

ATGAGGCGGC GACGAGCCCG GCTGGCCAGA GATGCAGGAG CCATGGAGTT GAGCTGCAGT 
GAAGTGCCTC TTTATGGACA GAAGACGGTC TATGCAAAGT TTGGCAAAAA CGTGTATCTA 
CCCGAAGATG CGGAGTTTTA CTTTGTTTAT GGCGGGTCGC ATCAGAGGCA CGTGGTGATT 
GCTGACCGTG TCCAGGACAA TGTCCTCCAA TCTAGCATTC CAGGCCACTG GCTTCAGGAG 
ACGGTGACGG TATCTGTGTG CCTCTGCTCG GAAGGGTACT CCCCAGTGAC CATGGGCTCT 
GGCTCAGTGA CCTACGTGGA CAACATGGCC TGCAGGCTGG CTCGTCTGCT GGTCACTCAG 
GCTGACCGAC TCACGGCCTG CAGTCACCAG ACGCTGCTGA CCCCGTTTGC CCTGACAGTG 
GAAGCGCTGC CTGCCTTGGA TGAGGAGCTC GTGCTGGCTC TGACCCAACT GGAGCTGCCT 
TTGGGTTGGA CAGTCCTGGG AAATTCCTCA CTTGAAGTAT CTCTACACAG AGAGTCTCTC 
CTGCATCTGG CTGTGAGATG GGCCCTCCCT AAGCTATTCC ACTTCCTCTT GTGTCTCCCT 
GGAGGAGTCA AGGCCTTGAA GCTACCTAAT GAAGAGGCCA CCACACCATT AGACTTAGCT 
TTGCAAGGTG GACACTCCAC ACTGGTAGAA GACATCACAA ATTTTCAGGG TAGCCATTCC 
CCAGGCTTCT CCCGACTGCG TCTTAACGAA GAGGCCACTC TGCAGTTTGT TCATTCATCG 
GAAACATTGA CCCTGACCGT TAACCACACA GCTGAGCATC TCTTGGAAGC AGACATCAAA 
CTCTTCCGGA AATATTTCTG GGACAGAGCC TTTCTTGTCA AGGCTCTTGA GCAAGAAGCC 
AAGACTGAGA AAGCAACCAT GCCTTCTGGA GCTGCAGAAA CTGAAGAAGA GGTTAGGAAT 
TTGGAGTCTG GCAGATCACC ATCGGAGGAG GAGGAGGATG CAAAGAGCAT CAAAAGCCAG 
GTGGATGGGC CGAGTGAACA CGAAGACCAG GACCGCCTAC CTCTGGATCG CTCCTTTGAT 
GGCCTCAAAA AATCCAAGCA TGTCCCAGCG TCCCTTGCTG CAGGCCAGCT CTCTGATGTT 
CTGAACGGGG GTGATGAAGT CTACGCTAAC TGTATGGTGA TTGACCAGGT TGGCGATTTG 
GATATCAACT ATATAAACCT AGAGGGACTC TCCACCCATA CGAGTCCCGA GTCTGGGAGG 
TCCATGCTGG GGCCTCAGGC TTGCATGCAC ACCCTTCCTC CCGACACCAG TCCCTGCGGG 
CGCCCACTCA TTGAGAACAG TGAGGGGACC CTGGATGCTG CAGCTTCGCA GTCTTTCGTG 
ACACCATCCA GTTCTCGCAC TTCCAACTTG AACCTTTCTT TTGGTCTCCA TGGATTTGAA 
AAGGAGCAGA GTCATCTCAA GAAAAGGAGT TCTAGCCTGG ATGCCCTGGT TGCCGACAGT 
GAGGGGGAAG GAGGTTCTGA GCCCCCCATC TGCTATGCTG TGGGCTCTCA GAGTTCCCCA 
AGAACTGGAC TCCCCAGCGG GGATGAACTG GACTCTTTTG AGACCAACAC TGAACCAGAC 
TGTAATATCT CCAGGACCGA ATCACTCTCT CTGTCCAGTA CTCTGCATTC AAAGGAGTCC 
TTGCTTTCTG GGATCCGGTC TCGCTCCTAC TCCTGCTCAT CACCCAAAAT CTCTTCAGGA 
AAATCCCGGC TAGTGCGCGA CTTCACAGTG TGCAGTACCA GCGAAGAACA GAGATCATAT 
AGTTTCCAGG AGCCACCAGG AGAAAAAAGG ATTCAGGAAG AAGAATGGGA TGAATACGTC 
ATTCCTGCCA AATCCGAGTC TGAAAAGTAC AAAGTGAGCC GAACTTTCAG CTTCCTCATG 
AACAGGATGA CCAGCCCACG GAACAAATCT AAGATGAAAA ACAAGGACAC TAAAGAAAAA 
GAAAAAATGA ATCGACATCA GTTTGTCCCG GGAACGTTCT CCGGGGTTCT GCAGTGCTCC 
GGTTGTGATA AAACACTCCT GGGGAAAGAG TCACTCCAGT GTGCCAACTG TAAGGCAAAC 
ACACACAAGG GCTGCAAAGA TGCTGTGCCT CCGTGTACCA AGAAATTCCA AGAGAAATAC 
AACAAGAACA AACCACAGAG CATCCTTGGA AGCTCTTCAG TCAGAGACGT CCCAGCACCT 
GGGCTCTCCT TGCACCCTTC TTCCTCTATG CCCATCGGAC TGCCAGCTGG AAGGAAGGAA 
TTTGCGGCTC AGGTGCATCC ACTGTCCAGA AGTGTCCCCG GCACCACTTT GGAAAGCTTC 
AGGAGGGCAG TGACATCGTT AGAGTCTGAG GGTGACAGCT GGAGAAGCAG GTCTCATTCC 
GATGAGCTGT TCCAGTCTAT GGGCTCTTCT CCTTCCACGG AGTCATTCAT GATGGAAGAT 
GTTGTGGATT CTTCTCTGTG GATCGACCTC AGTAGTGATG CTCAGGAGTT TGAAGCAGAG 
TCTTGGAGTC TCGTGGTGGA CCCTTCCTTT TGCAGTAGGC AGGAGAAGGA TGTCATCAAA 
AGACAAGATG TCATTTTTGA GCTGATGCAG ACAGAGGTCC ACCACATCCA GACTCTGCTC 
ATCATGTCCG AGGTCTTCAG GAAAGGCATG AAGGAGGAAC TGCAGCTGGA CCACAGCACG 
GTGGACAAGA TCTTCCCCTG CTTAGATGAG CTGCTGGAGA CTCACCGGCA CTTCTTCTTC 
AGCATGAAGG AGCGCAGGCA GGAGTCCTGC GCTGGCAGTG ACAGGAACTT TGTGATCAAC 
CAAATTGGAG ACATTCTGGT ACAACAGTTT TCAGAGGAAA ATGCAAGCAA AATGAAGAGG 
ATCTATGGAG AATTCTGCAG CCATCACAAA GAAGCCATGA GTCTCTTTAA AGAGCTGCAG 
CAGAACAAGA AGTTTCAGAA TTTTATTAAG ATCCGAAATA GCAACCTGTT GGCTCGTCGC 
CGCGGAATCC CGGAGTGCAT CCTGCTAGTT ACCCAGCGCA TCACAAAGTA CCCAGTCTTG 
GTGGAAAGGA TCCTGCAGTA CACCAAGGAA AGGACTGAAG AGCACCGGGA CCTGTGCAAA 
GCTCTCGGCT TAATTAAAGA CATGATCGCA GCCGTGGACC TGAAAGTCAG CGAATACGAG 
AAGAACCAAA AATGGCTCGA GATCCTAAAT AAAATCGAGA ACAAAACATA CACAAAGCTC 
AAAAATGGCC ACGTGTTTAG AAAGCAGGCG CTGCTGAGCC AGGAACGGGC TCTCCTGCAT 
GATGGGCTTG TCTACTGGAA AACCGCTACG GGTCGCTTCA AAGACATCCT GGCTCTGCTA 
CTAACTGACG TGCTGCTCTT CTTACAAGAA AAAGACCAGA AATACATCTT TGCAGCTGTT 
GACCAGAAGC CATCAGTGAT TTCCCTCCAG AAGCTGATTG CTAGAGAAGT TGCCAACGAG 
GAGAGAGGCA TGTTTCTGAT CAGTGCTTCG TCAGCCGGCC CCGAGATGTA CGAAATCCAC 
ACCAATTCCA AGGAGGAGCG GAATAACTGG ATGAGGCGGA TCCAGCAGGC AGTAGAAAGT 
TGTCCAGAGG AAGAAGGAGG AAGGACGAGC GAGTCGGATG AAGAGAGGCG GAAAGCTGAA 
GCTAGAGTGG CCAAGATTCA GCAATGTCAA GAAATACTCA GTAACCAAGA CCAACAGATT 
TGCACGTACT TGGAAGAAAA GCTGCATATC TACGCTGAAC TTGGGGAACT GAGTGGCTTT 
GAGGATGTCC ACCTAGAACC CCATCTGCTC ATCAAACCTG ACCCTGGAGA GCCTCCCCAG 
GCAGCGTCCC TGCTTGCAGC AGCCCTGAGA GAAGCCGAGA GTCTGCAAGT GGCTGTGAAG 
GCTTCAAAGA TGGGCGATGT AAGCCAGTCA TCCGAAGAAA GCCCTGGAGG GACTGTCCTG 
ATGGACACAC CCAGTACACA GGATGTACCA GCGTCACCAA CTGCTTCATT AGTCACAGAA 
GGGACAGAAG GAAGAGGCTG TTGGGATGTG GATCCCGGGC TCCAGGGTGT GGTAACCGAC 
TTGGCAGTCT CTGATGCAGG GGAGAAGGTG GAATATAGAA GTTTCTCCGG TTCTTCACAG 
TCAGAGATTA TACAAGCAAT ACAGAATTTA ACGCGTCTCC TCTACAGCCT TCAGGCTGCC 
TTGACCATCC AGGACAGTCA CATTGAGATC CACAAACTGG TTCTACAGCA GCGGGAGAGC 
TTGGCCCCTA GCCACTCCTT CCGAGGTGGC CCCTTGCAAG ACCAGGAGAA ATCCCGCTAC 
CTGGAAAAGC AGCGTGAAGA GCTGGCCAAC ATCCACAAGC TTCAACACCA GTTCCAGCAG 
GAGCAGCGGC GCTGGCACCG CACGTGCGAC CAGCAACAGC GCGAGCAGGA AGCCCAAGAG 
AGCTGGCTGC AGGCGCGGGA ACGTGAGTGC CAGTCCCAGG AGGAATTGTT GCTGCGCCAC 
CGTAGCGAGC TGGACCACCA ACTCCAGGAG TACCAGCAGA GCCTTGAGCG GCTACGGGAG 
GGCCAGCGGA TGGTGGAGAG GGAGAGACAG AAGATGCGTG TCCAGCAGGG TCTGCTGGGC 
CACTGCAAGC ATAGTCGCCA GCGGAGCCTC CCAGCCGTCT TCTCTCCAGG GAGCAAGGAG 
GTAACAGAAC TTAACCGAGC TGAGAGTTTG TGTCATGAAA ATTCATTTTT CATCAATGAA 
GCTTTTGGAC ACATGTCACT TAACACGTCC AATAAACCAA ACCCATCAGG GGTCCCCTGG 
GATGCTCACC CCCTGGAGGG GTCTCATTTT GATTTGGCAA GGACTAGTGA GAGTCCAACA 
GAACTGAAGA TAGACATTTC TCAGCCTCCT GATGTCAACT CTGAACTCTG GACAACGGGG 
CCGGGCCATC AGAGACCTGC TCTCCAAGAA AACAGCAAGG AGTCTTATAA AAATGTTGCA 
GATTTGGACT CCTTCCAGAG TGAATCGTCA TCTCCCCAGG ACTCCAATCA GCGTGGCCCC 
CAGCCACAGA CACTCACAAC AGAAGCAAAA CTAAGTCTTC CGATGGCGGC TGGACACGGT 
GGCGATGCTG GAGACGGGGC TGAAGAAAAC ATTCTTTACC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgef28 ( XM_017315361.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170850.1 Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X2, mRNA.

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CloneID	OMu78409
Clone ID Related Accession (Same CDS sequence)	XM_017315361.1 , XM_017315361.2
Accession Version	XM_017315361.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	rho guanine nucleotide exchange factor 28 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTCTG GCTCAGTGAC CTACGTGGAC AACATGGCCT GCAGGCTGGC TCGTCTGCTG 
GTCACTCAGG CTGACCGACT CACGGCCTGC AGTCACCAGA CGCTGCTGAC CCCGTTTGCC 
CTGACAGTGG AAGCGCTGCC TGCCTTGGAT GAGGAGCTCG TGCTGGCTCT GACCCAACTG 
GAGCTGCCTT TGGGTTGGAC AGTCCTGGGA AATTCCTCAC TTGAAGTATC TCTACACAGA 
GAGTCTCTCC TGCATCTGGC TGTGAGATGG GCCCTCCCTA AGCTATTCCA CTTCCTCTTG 
TGTCTCCCTG GAGGAGTCAA GGCCTTGAAG CTACCTAATG AAGAGGCCAC CACACCATTA 
GACTTAGCTT TGCAAGGTGG ACACTCCACA CTGGTAGAAG ACATCACAAA TTTTCAGGGT 
AGCCATTCCC CAGGCTTCTC CCGACTGCGT CTTAACGAAG AGGCCACTCT GCAGTTTGTT 
CATTCATCGG AAACATTGAC CCTGACCGTT AACCACACAG CTGAGCATCT CTTGGAAGCA 
GACATCAAAC TCTTCCGGAA ATATTTCTGG GACAGAGCCT TTCTTGTCAA GGCTCTTGAG 
CAAGAAGCCA AGACTGAGAA AGCAACCATG CCTTCTGGAG CTGCAGAAAC TGAAGAAGAG 
GTTAGGAATT TGGAGTCTGG CAGATCACCA TCGGAGGAGG AGGAGGATGC AAAGAGCATC 
AAAAGCCAGG TGGATGGGCC GAGTGAACAC GAAGACCAGG ACCGCCTACC TCTGGATCGC 
TCCTTTGATG GCCTCAAAAA ATCCAAGCAT GTCCCAGCGT CCCTTGCTGC AGGCCAGCTC 
TCTGATGTTC TGAACGGGGG TGATGAAGTC TACGCTAACT GTATGGTGAT TGACCAGGTT 
GGCGATTTGG ATATCAACTA TATAAACCTA GAGGGACTCT CCACCCATAC GAGTCCCGAG 
TCTGGGAGGT CCATGCTGGG GCCTCAGGCT TGCATGCACA CCCTTCCTCC CGACACCAGT 
CCCTGCGGGC GCCCACTCAT TGAGAACAGT GAGGGGACCC TGGATGCTGC AGCTTCGCAG 
TCTTTCGTGA CACCATCCAG TTCTCGCACT TCCAACTTGA ACCTTTCTTT TGGTCTCCAT 
GGATTTGAAA AGGAGCAGAG TCATCTCAAG AAAAGGAGTT CTAGCCTGGA TGCCCTGGTT 
GCCGACAGTG AGGGGGAAGG AGGTTCTGAG CCCCCCATCT GCTATGCTGT GGGCTCTCAG 
AGTTCCCCAA GAACTGGACT CCCCAGCGGG GATGAACTGG ACTCTTTTGA GACCAACACT 
GAACCAGACT GTAATATCTC CAGGACCGAA TCACTCTCTC TGTCCAGTAC TCTGCATTCA 
AAGGAGTCCT TGCTTTCTGG GATCCGGTCT CGCTCCTACT CCTGCTCATC ACCCAAAATC 
TCTTCAGGAA AATCCCGGCT AGTGCGCGAC TTCACAGTGT GCAGTACCAG CGAAGAACAG 
AGATCATATA GTTTCCAGGA GCCACCAGGA GAAAAAAGGA TTCAGGAAGA AGAATGGGAT 
GAATACGTCA TTCCTGCCAA ATCCGAGTCT GAAAAGTACA AAGTGAGCCG AACTTTCAGC 
TTCCTCATGA ACAGGATGAC CAGCCCACGG AACAAATCTA AGATGAAAAA CAAGGACACT 
AAAGAAAAAG AAAAAATGAA TCGACATCAG TTTGTCCCGG GAACGTTCTC CGGGGTTCTG 
CAGTGCTCCG GTTGTGATAA AACACTCCTG GGGAAAGAGT CACTCCAGTG TGCCAACTGT 
AAGGCAAACA CACACAAGGG CTGCAAAGAT GCTGTGCCTC CGTGTACCAA GAAATTCCAA 
GAGAAATACA ACAAGAACAA ACCACAGAGC ATCCTTGGAA GCTCTTCAGT CAGAGACGTC 
CCAGCACCTG GGCTCTCCTT GCACCCTTCT TCCTCTATGC CCATCGGACT GCCAGCTGGA 
AGGAAGGAAT TTGCGGCTCA GGTGCATCCA CTGTCCAGAA GTGTCCCCGG CACCACTTTG 
GAAAGCTTCA GGAGGGCAGT GACATCGTTA GAGTCTGAGG GTGACAGCTG GAGAAGCAGG 
TCTCATTCCG ATGAGCTGTT CCAGTCTATG GGCTCTTCTC CTTCCACGGA GTCATTCATG 
ATGGAAGATG TTGTGGATTC TTCTCTGTGG ATCGACCTCA GTAGTGATGC TCAGGAGTTT 
GAAGCAGAGT CTTGGAGTCT CGTGGTGGAC CCTTCCTTTT GCAGTAGGCA GGAGAAGGAT 
GTCATCAAAA GACAAGATGT CATTTTTGAG CTGATGCAGA CAGAGGTCCA CCACATCCAG 
ACTCTGCTCA TCATGTCCGA GGTCTTCAGG AAAGGCATGA AGGAGGAACT GCAGCTGGAC 
CACAGCACGG TGGACAAGAT CTTCCCCTGC TTAGATGAGC TGCTGGAGAC TCACCGGCAC 
TTCTTCTTCA GCATGAAGGA GCGCAGGCAG GAGTCCTGCG CTGGCAGTGA CAGGAACTTT 
GTGATCAACC AAATTGGAGA CATTCTGGTA CAACAGTTTT CAGAGGAAAA TGCAAGCAAA 
ATGAAGAGGA TCTATGGAGA ATTCTGCAGC CATCACAAAG AAGCCATGAG TCTCTTTAAA 
GAGCTGCAGC AGAACAAGAA GTTTCAGAAT TTTATTAAGA TCCGAAATAG CAACCTGTTG 
GCTCGTCGCC GCGGAATCCC GGAGTGCATC CTGCTAGTTA CCCAGCGCAT CACAAAGTAC 
CCAGTCTTGG TGGAAAGGAT CCTGCAGTAC ACCAAGGAAA GGACTGAAGA GCACCGGGAC 
CTGTGCAAAG CTCTCGGCTT AATTAAAGAC ATGATCGCAG CCGTGGACCT GAAAGTCAGC 
GAATACGAGA AGAACCAAAA ATGGCTCGAG ATCCTAAATA AAATCGAGAA CAAAACATAC 
ACAAAGCTCA AAAATGGCCA CGTGTTTAGA AAGCAGGCGC TGCTGAGCCA GGAACGGGCT 
CTCCTGCATG ATGGGCTTGT CTACTGGAAA ACCGCTACGG GTCGCTTCAA AGACATCCTG 
GCTCTGCTAC TAACTGACGT GCTGCTCTTC TTACAAGAAA AAGACCAGAA ATACATCTTT 
GCAGCTGTTG ACCAGAAGCC ATCAGTGATT TCCCTCCAGA AGCTGATTGC TAGAGAAGTT 
GCCAACGAGG AGAGAGGCAT GTTTCTGATC AGTGCTTCGT CAGCCGGCCC CGAGATGTAC 
GAAATCCACA CCAATTCCAA GGAGGAGCGG AATAACTGGA TGAGGCGGAT CCAGCAGGCA 
GTAGAAAGTT GTCCAGAGGA AGAAGGAGGA AGGACGAGCG AGTCGGATGA AGAGAGGCGG 
AAAGCTGAAG CTAGAGTGGC CAAGATTCAG CAATGTCAAG AAATACTCAG TAACCAAGAC 
CAACAGATTT GCACGTACTT GGAAGAAAAG CTGCATATCT ACGCTGAACT TGGGGAACTG 
AGTGGCTTTG AGGATGTCCA CCTAGAACCC CATCTGCTCA TCAAACCTGA CCCTGGAGAG 
CCTCCCCAGG CAGCGTCCCT GCTTGCAGCA GCCCTGAGAG AAGCCGAGAG TCTGCAAGTG 
GCTGTGAAGG CTTCAAAGAT GGGCGATGTA AGCCAGTCAT CCGAAGAAAG CCCTGGAGGG 
ACTGTCCTGA TGGACACACC CAGTACACAG GATGTACCAG CGTCACCAAC TGCTTCATTA 
GTCACAGAAG GGACAGAAGG AAGAGGCTGT TGGGATGTGG ATCCCGGGCT CCAGGGTGTG 
GTAACCGACT TGGCAGTCTC TGATGCAGGG GAGAAGGTGG AATATAGAAG TTTCTCCGGT 
TCTTCACAGT CAGAGATTAT ACAAGCAATA CAGAATTTAA CGCGTCTCCT CTACAGCCTT 
CAGGCTGCCT TGACCATCCA GGACAGTCAC ATTGAGATCC ACAAACTGGT TCTACAGCAG 
CGGGAGAGCT TGGCCCCTAG CCACTCCTTC CGAGGTGGCC CCTTGCAAGA CCAGGAGAAA 
TCCCGCTACC TGGAAAAGCA GCGTGAAGAG CTGGCCAACA TCCACAAGCT TCAACACCAG 
TTCCAGCAGG AGCAGCGGCG CTGGCACCGC ACGTGCGACC AGCAACAGCG CGAGCAGGAA 
GCCCAAGAGA GCTGGCTGCA GGCGCGGGAA CGTGAGTGCC AGTCCCAGGA GGAATTGTTG 
CTGCGCCACC GTAGCGAGCT GGACCACCAA CTCCAGGAGT ACCAGCAGAG CCTTGAGCGG 
CTACGGGAGG GCCAGCGGAT GGTGGAGAGG GAGAGACAGA AGATGCGTGT CCAGCAGGGT 
CTGCTGGGCC ACTGCAAGCA TAGTCGCCAG CGGAGCCTCC CAGCCGTCTT CTCTCCAGGG 
AGCAAGGAGG TAACAGAACT TAACCGAGCT GAGAGTTTGT GTCATGAAAA TTCATTTTTC 
ATCAATGAAG CTTTTGGACA CATGTCACTT AACACGTCCA ATAAACCAAA CCCATCAGGG 
GTCCCCTGGG ATGCTCACCC CCTGGAGGGG TCTCATTTTG ATTTGGCAAG GACTAGTGAG 
AGTCCAACAG AACTGAAGAT AGACATTTCT CAGCCTCCTG ATGTCAACTC TGAACTCTGG 
ACAACGGGGC CGGGCCATCA GAGACCTGCT CTCCAAGAAA ACAGCAAGGA GTCTTATAAA 
AATGTTGCAG ATTTGGACTC CTTCCAGAGT GAATCGTCAT CTCCCCAGGA CTCCAATCAG 
CGTGGCCCCC AGCCACAGAC ACTCACAACA GAAGCAAAAC TAAGTCTTCC GATGGCGGCT 
GGACACGGTG GCGATGCTGG AGACGGGGCT GAAGAAAACA TTCTTTACCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170850.1
CDS	236..5089
Translation	MGSGSVTYVDNMACRLARLLVTQADRLTACSHQTLLTPFALTVEALPALDEELVLALTQLELPLGWTVLGNSSLEVSLHRESLLHLAVRWALPKLFHFLLCLPGGVKALKLPNEEATTPLDLALQGGHSTLVEDITNFQGSHSPGFSRLRLNEEATLQFVHSSETLTLTVNHTAEHLLEADIKLFRKYFWDRAFLVKALEQEAKTEKATMPSGAAETEEEVRNLESGRSPSEEEEDAKSIKSQVDGPSEHEDQDRLPLDRSFDGLKKSKHVPASLAAGQLSDVLNGGDEVYANCMVIDQVGDLDINYINLEGLSTHTSPESGRSMLGPQACMHTLPPDTSPCGRPLIENSEGTLDAAASQSFVTPSSSRTSNLNLSFGLHGFEKEQSHLKKRSSSLDALVADSEGEGGSEPPICYAVGSQSSPRTGLPSGDELDSFETNTEPDCNISRTESLSLSSTLHSKESLLSGIRSRSYSCSSPKISSGKSRLVRDFTVCSTSEEQRSYSFQEPPGEKRIQEEEWDEYVIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKMKNKDTKEKEKMNRHQFVPGTFSGVLQCSGCDKTLLGKESLQCANCKANTHKGCKDAVPPCTKKFQEKYNKNKPQSILGSSSVRDVPAPGLSLHPSSSMPIGLPAGRKEFAAQVHPLSRSVPGTTLESFRRAVTSLESEGDSWRSRSHSDELFQSMGSSPSTESFMMEDVVDSSLWIDLSSDAQEFEAESWSLVVDPSFCSRQEKDVIKRQDVIFELMQTEVHHIQTLLIMSEVFRKGMKEELQLDHSTVDKIFPCLDELLETHRHFFFSMKERRQESCAGSDRNFVINQIGDILVQQFSEENASKMKRIYGEFCSHHKEAMSLFKELQQNKKFQNFIKIRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKERTEEHRDLCKALGLIKDMIAAVDLKVSEYEKNQKWLEILNKIENKTYTKLKNGHVFRKQALLSQERALLHDGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNNWMRRIQQAVESCPEEEGGRTSESDEERRKAEARVAKIQQCQEILSNQDQQICTYLEEKLHIYAELGELSGFEDVHLEPHLLIKPDPGEPPQAASLLAAALREAESLQVAVKASKMGDVSQSSEESPGGTVLMDTPSTQDVPASPTASLVTEGTEGRGCWDVDPGLQGVVTDLAVSDAGEKVEYRSFSGSSQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIHKLVLQQRESLAPSHSFRGGPLQDQEKSRYLEKQREELANIHKLQHQFQQEQRRWHRTCDQQQREQEAQESWLQARERECQSQEELLLRHRSELDHQLQEYQQSLERLREGQRMVERERQKMRVQQGLLGHCKHSRQRSLPAVFSPGSKEVTELNRAESLCHENSFFINEAFGHMSLNTSNKPNPSGVPWDAHPLEGSHFDLARTSESPTELKIDISQPPDVNSELWTTGPGHQRPALQENSKESYKNVADLDSFQSESSSPQDSNQRGPQPQTLTTEAKLSLPMAAGHGGDAGDGAEENILYL

Target ORF information:

RefSeq Version	XM_017315361.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315361.1

ORF Insert Sequence:

ATGGGCTCTG GCTCAGTGAC CTACGTGGAC AACATGGCCT GCAGGCTGGC TCGTCTGCTG 
GTCACTCAGG CTGACCGACT CACGGCCTGC AGTCACCAGA CGCTGCTGAC CCCGTTTGCC 
CTGACAGTGG AAGCGCTGCC TGCCTTGGAT GAGGAGCTCG TGCTGGCTCT GACCCAACTG 
GAGCTGCCTT TGGGTTGGAC AGTCCTGGGA AATTCCTCAC TTGAAGTATC TCTACACAGA 
GAGTCTCTCC TGCATCTGGC TGTGAGATGG GCCCTCCCTA AGCTATTCCA CTTCCTCTTG 
TGTCTCCCTG GAGGAGTCAA GGCCTTGAAG CTACCTAATG AAGAGGCCAC CACACCATTA 
GACTTAGCTT TGCAAGGTGG ACACTCCACA CTGGTAGAAG ACATCACAAA TTTTCAGGGT 
AGCCATTCCC CAGGCTTCTC CCGACTGCGT CTTAACGAAG AGGCCACTCT GCAGTTTGTT 
CATTCATCGG AAACATTGAC CCTGACCGTT AACCACACAG CTGAGCATCT CTTGGAAGCA 
GACATCAAAC TCTTCCGGAA ATATTTCTGG GACAGAGCCT TTCTTGTCAA GGCTCTTGAG 
CAAGAAGCCA AGACTGAGAA AGCAACCATG CCTTCTGGAG CTGCAGAAAC TGAAGAAGAG 
GTTAGGAATT TGGAGTCTGG CAGATCACCA TCGGAGGAGG AGGAGGATGC AAAGAGCATC 
AAAAGCCAGG TGGATGGGCC GAGTGAACAC GAAGACCAGG ACCGCCTACC TCTGGATCGC 
TCCTTTGATG GCCTCAAAAA ATCCAAGCAT GTCCCAGCGT CCCTTGCTGC AGGCCAGCTC 
TCTGATGTTC TGAACGGGGG TGATGAAGTC TACGCTAACT GTATGGTGAT TGACCAGGTT 
GGCGATTTGG ATATCAACTA TATAAACCTA GAGGGACTCT CCACCCATAC GAGTCCCGAG 
TCTGGGAGGT CCATGCTGGG GCCTCAGGCT TGCATGCACA CCCTTCCTCC CGACACCAGT 
CCCTGCGGGC GCCCACTCAT TGAGAACAGT GAGGGGACCC TGGATGCTGC AGCTTCGCAG 
TCTTTCGTGA CACCATCCAG TTCTCGCACT TCCAACTTGA ACCTTTCTTT TGGTCTCCAT 
GGATTTGAAA AGGAGCAGAG TCATCTCAAG AAAAGGAGTT CTAGCCTGGA TGCCCTGGTT 
GCCGACAGTG AGGGGGAAGG AGGTTCTGAG CCCCCCATCT GCTATGCTGT GGGCTCTCAG 
AGTTCCCCAA GAACTGGACT CCCCAGCGGG GATGAACTGG ACTCTTTTGA GACCAACACT 
GAACCAGACT GTAATATCTC CAGGACCGAA TCACTCTCTC TGTCCAGTAC TCTGCATTCA 
AAGGAGTCCT TGCTTTCTGG GATCCGGTCT CGCTCCTACT CCTGCTCATC ACCCAAAATC 
TCTTCAGGAA AATCCCGGCT AGTGCGCGAC TTCACAGTGT GCAGTACCAG CGAAGAACAG 
AGATCATATA GTTTCCAGGA GCCACCAGGA GAAAAAAGGA TTCAGGAAGA AGAATGGGAT 
GAATACGTCA TTCCTGCCAA ATCCGAGTCT GAAAAGTACA AAGTGAGCCG AACTTTCAGC 
TTCCTCATGA ACAGGATGAC CAGCCCACGG AACAAATCTA AGATGAAAAA CAAGGACACT 
AAAGAAAAAG AAAAAATGAA TCGACATCAG TTTGTCCCGG GAACGTTCTC CGGGGTTCTG 
CAGTGCTCCG GTTGTGATAA AACACTCCTG GGGAAAGAGT CACTCCAGTG TGCCAACTGT 
AAGGCAAACA CACACAAGGG CTGCAAAGAT GCTGTGCCTC CGTGTACCAA GAAATTCCAA 
GAGAAATACA ACAAGAACAA ACCACAGAGC ATCCTTGGAA GCTCTTCAGT CAGAGACGTC 
CCAGCACCTG GGCTCTCCTT GCACCCTTCT TCCTCTATGC CCATCGGACT GCCAGCTGGA 
AGGAAGGAAT TTGCGGCTCA GGTGCATCCA CTGTCCAGAA GTGTCCCCGG CACCACTTTG 
GAAAGCTTCA GGAGGGCAGT GACATCGTTA GAGTCTGAGG GTGACAGCTG GAGAAGCAGG 
TCTCATTCCG ATGAGCTGTT CCAGTCTATG GGCTCTTCTC CTTCCACGGA GTCATTCATG 
ATGGAAGATG TTGTGGATTC TTCTCTGTGG ATCGACCTCA GTAGTGATGC TCAGGAGTTT 
GAAGCAGAGT CTTGGAGTCT CGTGGTGGAC CCTTCCTTTT GCAGTAGGCA GGAGAAGGAT 
GTCATCAAAA GACAAGATGT CATTTTTGAG CTGATGCAGA CAGAGGTCCA CCACATCCAG 
ACTCTGCTCA TCATGTCCGA GGTCTTCAGG AAAGGCATGA AGGAGGAACT GCAGCTGGAC 
CACAGCACGG TGGACAAGAT CTTCCCCTGC TTAGATGAGC TGCTGGAGAC TCACCGGCAC 
TTCTTCTTCA GCATGAAGGA GCGCAGGCAG GAGTCCTGCG CTGGCAGTGA CAGGAACTTT 
GTGATCAACC AAATTGGAGA CATTCTGGTA CAACAGTTTT CAGAGGAAAA TGCAAGCAAA 
ATGAAGAGGA TCTATGGAGA ATTCTGCAGC CATCACAAAG AAGCCATGAG TCTCTTTAAA 
GAGCTGCAGC AGAACAAGAA GTTTCAGAAT TTTATTAAGA TCCGAAATAG CAACCTGTTG 
GCTCGTCGCC GCGGAATCCC GGAGTGCATC CTGCTAGTTA CCCAGCGCAT CACAAAGTAC 
CCAGTCTTGG TGGAAAGGAT CCTGCAGTAC ACCAAGGAAA GGACTGAAGA GCACCGGGAC 
CTGTGCAAAG CTCTCGGCTT AATTAAAGAC ATGATCGCAG CCGTGGACCT GAAAGTCAGC 
GAATACGAGA AGAACCAAAA ATGGCTCGAG ATCCTAAATA AAATCGAGAA CAAAACATAC 
ACAAAGCTCA AAAATGGCCA CGTGTTTAGA AAGCAGGCGC TGCTGAGCCA GGAACGGGCT 
CTCCTGCATG ATGGGCTTGT CTACTGGAAA ACCGCTACGG GTCGCTTCAA AGACATCCTG 
GCTCTGCTAC TAACTGACGT GCTGCTCTTC TTACAAGAAA AAGACCAGAA ATACATCTTT 
GCAGCTGTTG ACCAGAAGCC ATCAGTGATT TCCCTCCAGA AGCTGATTGC TAGAGAAGTT 
GCCAACGAGG AGAGAGGCAT GTTTCTGATC AGTGCTTCGT CAGCCGGCCC CGAGATGTAC 
GAAATCCACA CCAATTCCAA GGAGGAGCGG AATAACTGGA TGAGGCGGAT CCAGCAGGCA 
GTAGAAAGTT GTCCAGAGGA AGAAGGAGGA AGGACGAGCG AGTCGGATGA AGAGAGGCGG 
AAAGCTGAAG CTAGAGTGGC CAAGATTCAG CAATGTCAAG AAATACTCAG TAACCAAGAC 
CAACAGATTT GCACGTACTT GGAAGAAAAG CTGCATATCT ACGCTGAACT TGGGGAACTG 
AGTGGCTTTG AGGATGTCCA CCTAGAACCC CATCTGCTCA TCAAACCTGA CCCTGGAGAG 
CCTCCCCAGG CAGCGTCCCT GCTTGCAGCA GCCCTGAGAG AAGCCGAGAG TCTGCAAGTG 
GCTGTGAAGG CTTCAAAGAT GGGCGATGTA AGCCAGTCAT CCGAAGAAAG CCCTGGAGGG 
ACTGTCCTGA TGGACACACC CAGTACACAG GATGTACCAG CGTCACCAAC TGCTTCATTA 
GTCACAGAAG GGACAGAAGG AAGAGGCTGT TGGGATGTGG ATCCCGGGCT CCAGGGTGTG 
GTAACCGACT TGGCAGTCTC TGATGCAGGG GAGAAGGTGG AATATAGAAG TTTCTCCGGT 
TCTTCACAGT CAGAGATTAT ACAAGCAATA CAGAATTTAA CGCGTCTCCT CTACAGCCTT 
CAGGCTGCCT TGACCATCCA GGACAGTCAC ATTGAGATCC ACAAACTGGT TCTACAGCAG 
CGGGAGAGCT TGGCCCCTAG CCACTCCTTC CGAGGTGGCC CCTTGCAAGA CCAGGAGAAA 
TCCCGCTACC TGGAAAAGCA GCGTGAAGAG CTGGCCAACA TCCACAAGCT TCAACACCAG 
TTCCAGCAGG AGCAGCGGCG CTGGCACCGC ACGTGCGACC AGCAACAGCG CGAGCAGGAA 
GCCCAAGAGA GCTGGCTGCA GGCGCGGGAA CGTGAGTGCC AGTCCCAGGA GGAATTGTTG 
CTGCGCCACC GTAGCGAGCT GGACCACCAA CTCCAGGAGT ACCAGCAGAG CCTTGAGCGG 
CTACGGGAGG GCCAGCGGAT GGTGGAGAGG GAGAGACAGA AGATGCGTGT CCAGCAGGGT 
CTGCTGGGCC ACTGCAAGCA TAGTCGCCAG CGGAGCCTCC CAGCCGTCTT CTCTCCAGGG 
AGCAAGGAGG TAACAGAACT TAACCGAGCT GAGAGTTTGT GTCATGAAAA TTCATTTTTC 
ATCAATGAAG CTTTTGGACA CATGTCACTT AACACGTCCA ATAAACCAAA CCCATCAGGG 
GTCCCCTGGG ATGCTCACCC CCTGGAGGGG TCTCATTTTG ATTTGGCAAG GACTAGTGAG 
AGTCCAACAG AACTGAAGAT AGACATTTCT CAGCCTCCTG ATGTCAACTC TGAACTCTGG 
ACAACGGGGC CGGGCCATCA GAGACCTGCT CTCCAAGAAA ACAGCAAGGA GTCTTATAAA 
AATGTTGCAG ATTTGGACTC CTTCCAGAGT GAATCGTCAT CTCCCCAGGA CTCCAATCAG 
CGTGGCCCCC AGCCACAGAC ACTCACAACA GAAGCAAAAC TAAGTCTTCC GATGGCGGCT 
GGACACGGTG GCGATGCTGG AGACGGGGCT GAAGAAAACA TTCTTTACCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgef28 ( XM_017315361.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170850.1 Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X2, mRNA.

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CloneID	OMu78409
Clone ID Related Accession (Same CDS sequence)	XM_017315361.1 , XM_017315361.2
Accession Version	XM_017315361.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rho guanine nucleotide exchange factor 28 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017315361.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTCTG GCTCAGTGAC CTACGTGGAC AACATGGCCT GCAGGCTGGC TCGTCTGCTG 
GTCACTCAGG CTGACCGACT CACGGCCTGC AGTCACCAGA CGCTGCTGAC CCCGTTTGCC 
CTGACAGTGG AAGCGCTGCC TGCCTTGGAT GAGGAGCTCG TGCTGGCTCT GACCCAACTG 
GAGCTGCCTT TGGGTTGGAC AGTCCTGGGA AATTCCTCAC TTGAAGTATC TCTACACAGA 
GAGTCTCTCC TGCATCTGGC TGTGAGATGG GCCCTCCCTA AGCTATTCCA CTTCCTCTTG 
TGTCTCCCTG GAGGAGTCAA GGCCTTGAAG CTACCTAATG AAGAGGCCAC CACACCATTA 
GACTTAGCTT TGCAAGGTGG ACACTCCACA CTGGTAGAAG ACATCACAAA TTTTCAGGGT 
AGCCATTCCC CAGGCTTCTC CCGACTGCGT CTTAACGAAG AGGCCACTCT GCAGTTTGTT 
CATTCATCGG AAACATTGAC CCTGACCGTT AACCACACAG CTGAGCATCT CTTGGAAGCA 
GACATCAAAC TCTTCCGGAA ATATTTCTGG GACAGAGCCT TTCTTGTCAA GGCTCTTGAG 
CAAGAAGCCA AGACTGAGAA AGCAACCATG CCTTCTGGAG CTGCAGAAAC TGAAGAAGAG 
GTTAGGAATT TGGAGTCTGG CAGATCACCA TCGGAGGAGG AGGAGGATGC AAAGAGCATC 
AAAAGCCAGG TGGATGGGCC GAGTGAACAC GAAGACCAGG ACCGCCTACC TCTGGATCGC 
TCCTTTGATG GCCTCAAAAA ATCCAAGCAT GTCCCAGCGT CCCTTGCTGC AGGCCAGCTC 
TCTGATGTTC TGAACGGGGG TGATGAAGTC TACGCTAACT GTATGGTGAT TGACCAGGTT 
GGCGATTTGG ATATCAACTA TATAAACCTA GAGGGACTCT CCACCCATAC GAGTCCCGAG 
TCTGGGAGGT CCATGCTGGG GCCTCAGGCT TGCATGCACA CCCTTCCTCC CGACACCAGT 
CCCTGCGGGC GCCCACTCAT TGAGAACAGT GAGGGGACCC TGGATGCTGC AGCTTCGCAG 
TCTTTCGTGA CACCATCCAG TTCTCGCACT TCCAACTTGA ACCTTTCTTT TGGTCTCCAT 
GGATTTGAAA AGGAGCAGAG TCATCTCAAG AAAAGGAGTT CTAGCCTGGA TGCCCTGGTT 
GCCGACAGTG AGGGGGAAGG AGGTTCTGAG CCCCCCATCT GCTATGCTGT GGGCTCTCAG 
AGTTCCCCAA GAACTGGACT CCCCAGCGGG GATGAACTGG ACTCTTTTGA GACCAACACT 
GAACCAGACT GTAATATCTC CAGGACCGAA TCACTCTCTC TGTCCAGTAC TCTGCATTCA 
AAGGAGTCCT TGCTTTCTGG GATCCGGTCT CGCTCCTACT CCTGCTCATC ACCCAAAATC 
TCTTCAGGAA AATCCCGGCT AGTGCGCGAC TTCACAGTGT GCAGTACCAG CGAAGAACAG 
AGATCATATA GTTTCCAGGA GCCACCAGGA GAAAAAAGGA TTCAGGAAGA AGAATGGGAT 
GAATACGTCA TTCCTGCCAA ATCCGAGTCT GAAAAGTACA AAGTGAGCCG AACTTTCAGC 
TTCCTCATGA ACAGGATGAC CAGCCCACGG AACAAATCTA AGATGAAAAA CAAGGACACT 
AAAGAAAAAG AAAAAATGAA TCGACATCAG TTTGTCCCGG GAACGTTCTC CGGGGTTCTG 
CAGTGCTCCG GTTGTGATAA AACACTCCTG GGGAAAGAGT CACTCCAGTG TGCCAACTGT 
AAGGCAAACA CACACAAGGG CTGCAAAGAT GCTGTGCCTC CGTGTACCAA GAAATTCCAA 
GAGAAATACA ACAAGAACAA ACCACAGAGC ATCCTTGGAA GCTCTTCAGT CAGAGACGTC 
CCAGCACCTG GGCTCTCCTT GCACCCTTCT TCCTCTATGC CCATCGGACT GCCAGCTGGA 
AGGAAGGAAT TTGCGGCTCA GGTGCATCCA CTGTCCAGAA GTGTCCCCGG CACCACTTTG 
GAAAGCTTCA GGAGGGCAGT GACATCGTTA GAGTCTGAGG GTGACAGCTG GAGAAGCAGG 
TCTCATTCCG ATGAGCTGTT CCAGTCTATG GGCTCTTCTC CTTCCACGGA GTCATTCATG 
ATGGAAGATG TTGTGGATTC TTCTCTGTGG ATCGACCTCA GTAGTGATGC TCAGGAGTTT 
GAAGCAGAGT CTTGGAGTCT CGTGGTGGAC CCTTCCTTTT GCAGTAGGCA GGAGAAGGAT 
GTCATCAAAA GACAAGATGT CATTTTTGAG CTGATGCAGA CAGAGGTCCA CCACATCCAG 
ACTCTGCTCA TCATGTCCGA GGTCTTCAGG AAAGGCATGA AGGAGGAACT GCAGCTGGAC 
CACAGCACGG TGGACAAGAT CTTCCCCTGC TTAGATGAGC TGCTGGAGAC TCACCGGCAC 
TTCTTCTTCA GCATGAAGGA GCGCAGGCAG GAGTCCTGCG CTGGCAGTGA CAGGAACTTT 
GTGATCAACC AAATTGGAGA CATTCTGGTA CAACAGTTTT CAGAGGAAAA TGCAAGCAAA 
ATGAAGAGGA TCTATGGAGA ATTCTGCAGC CATCACAAAG AAGCCATGAG TCTCTTTAAA 
GAGCTGCAGC AGAACAAGAA GTTTCAGAAT TTTATTAAGA TCCGAAATAG CAACCTGTTG 
GCTCGTCGCC GCGGAATCCC GGAGTGCATC CTGCTAGTTA CCCAGCGCAT CACAAAGTAC 
CCAGTCTTGG TGGAAAGGAT CCTGCAGTAC ACCAAGGAAA GGACTGAAGA GCACCGGGAC 
CTGTGCAAAG CTCTCGGCTT AATTAAAGAC ATGATCGCAG CCGTGGACCT GAAAGTCAGC 
GAATACGAGA AGAACCAAAA ATGGCTCGAG ATCCTAAATA AAATCGAGAA CAAAACATAC 
ACAAAGCTCA AAAATGGCCA CGTGTTTAGA AAGCAGGCGC TGCTGAGCCA GGAACGGGCT 
CTCCTGCATG ATGGGCTTGT CTACTGGAAA ACCGCTACGG GTCGCTTCAA AGACATCCTG 
GCTCTGCTAC TAACTGACGT GCTGCTCTTC TTACAAGAAA AAGACCAGAA ATACATCTTT 
GCAGCTGTTG ACCAGAAGCC ATCAGTGATT TCCCTCCAGA AGCTGATTGC TAGAGAAGTT 
GCCAACGAGG AGAGAGGCAT GTTTCTGATC AGTGCTTCGT CAGCCGGCCC CGAGATGTAC 
GAAATCCACA CCAATTCCAA GGAGGAGCGG AATAACTGGA TGAGGCGGAT CCAGCAGGCA 
GTAGAAAGTT GTCCAGAGGA AGAAGGAGGA AGGACGAGCG AGTCGGATGA AGAGAGGCGG 
AAAGCTGAAG CTAGAGTGGC CAAGATTCAG CAATGTCAAG AAATACTCAG TAACCAAGAC 
CAACAGATTT GCACGTACTT GGAAGAAAAG CTGCATATCT ACGCTGAACT TGGGGAACTG 
AGTGGCTTTG AGGATGTCCA CCTAGAACCC CATCTGCTCA TCAAACCTGA CCCTGGAGAG 
CCTCCCCAGG CAGCGTCCCT GCTTGCAGCA GCCCTGAGAG AAGCCGAGAG TCTGCAAGTG 
GCTGTGAAGG CTTCAAAGAT GGGCGATGTA AGCCAGTCAT CCGAAGAAAG CCCTGGAGGG 
ACTGTCCTGA TGGACACACC CAGTACACAG GATGTACCAG CGTCACCAAC TGCTTCATTA 
GTCACAGAAG GGACAGAAGG AAGAGGCTGT TGGGATGTGG ATCCCGGGCT CCAGGGTGTG 
GTAACCGACT TGGCAGTCTC TGATGCAGGG GAGAAGGTGG AATATAGAAG TTTCTCCGGT 
TCTTCACAGT CAGAGATTAT ACAAGCAATA CAGAATTTAA CGCGTCTCCT CTACAGCCTT 
CAGGCTGCCT TGACCATCCA GGACAGTCAC ATTGAGATCC ACAAACTGGT TCTACAGCAG 
CGGGAGAGCT TGGCCCCTAG CCACTCCTTC CGAGGTGGCC CCTTGCAAGA CCAGGAGAAA 
TCCCGCTACC TGGAAAAGCA GCGTGAAGAG CTGGCCAACA TCCACAAGCT TCAACACCAG 
TTCCAGCAGG AGCAGCGGCG CTGGCACCGC ACGTGCGACC AGCAACAGCG CGAGCAGGAA 
GCCCAAGAGA GCTGGCTGCA GGCGCGGGAA CGTGAGTGCC AGTCCCAGGA GGAATTGTTG 
CTGCGCCACC GTAGCGAGCT GGACCACCAA CTCCAGGAGT ACCAGCAGAG CCTTGAGCGG 
CTACGGGAGG GCCAGCGGAT GGTGGAGAGG GAGAGACAGA AGATGCGTGT CCAGCAGGGT 
CTGCTGGGCC ACTGCAAGCA TAGTCGCCAG CGGAGCCTCC CAGCCGTCTT CTCTCCAGGG 
AGCAAGGAGG TAACAGAACT TAACCGAGCT GAGAGTTTGT GTCATGAAAA TTCATTTTTC 
ATCAATGAAG CTTTTGGACA CATGTCACTT AACACGTCCA ATAAACCAAA CCCATCAGGG 
GTCCCCTGGG ATGCTCACCC CCTGGAGGGG TCTCATTTTG ATTTGGCAAG GACTAGTGAG 
AGTCCAACAG AACTGAAGAT AGACATTTCT CAGCCTCCTG ATGTCAACTC TGAACTCTGG 
ACAACGGGGC CGGGCCATCA GAGACCTGCT CTCCAAGAAA ACAGCAAGGA GTCTTATAAA 
AATGTTGCAG ATTTGGACTC CTTCCAGAGT GAATCGTCAT CTCCCCAGGA CTCCAATCAG 
CGTGGCCCCC AGCCACAGAC ACTCACAACA GAAGCAAAAC TAAGTCTTCC GATGGCGGCT 
GGACACGGTG GCGATGCTGG AGACGGGGCT GAAGAAAACA TTCTTTACCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170850.1
CDS	235..5088
Translation	MGSGSVTYVDNMACRLARLLVTQADRLTACSHQTLLTPFALTVEALPALDEELVLALTQLELPLGWTVLGNSSLEVSLHRESLLHLAVRWALPKLFHFLLCLPGGVKALKLPNEEATTPLDLALQGGHSTLVEDITNFQGSHSPGFSRLRLNEEATLQFVHSSETLTLTVNHTAEHLLEADIKLFRKYFWDRAFLVKALEQEAKTEKATMPSGAAETEEEVRNLESGRSPSEEEEDAKSIKSQVDGPSEHEDQDRLPLDRSFDGLKKSKHVPASLAAGQLSDVLNGGDEVYANCMVIDQVGDLDINYINLEGLSTHTSPESGRSMLGPQACMHTLPPDTSPCGRPLIENSEGTLDAAASQSFVTPSSSRTSNLNLSFGLHGFEKEQSHLKKRSSSLDALVADSEGEGGSEPPICYAVGSQSSPRTGLPSGDELDSFETNTEPDCNISRTESLSLSSTLHSKESLLSGIRSRSYSCSSPKISSGKSRLVRDFTVCSTSEEQRSYSFQEPPGEKRIQEEEWDEYVIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKMKNKDTKEKEKMNRHQFVPGTFSGVLQCSGCDKTLLGKESLQCANCKANTHKGCKDAVPPCTKKFQEKYNKNKPQSILGSSSVRDVPAPGLSLHPSSSMPIGLPAGRKEFAAQVHPLSRSVPGTTLESFRRAVTSLESEGDSWRSRSHSDELFQSMGSSPSTESFMMEDVVDSSLWIDLSSDAQEFEAESWSLVVDPSFCSRQEKDVIKRQDVIFELMQTEVHHIQTLLIMSEVFRKGMKEELQLDHSTVDKIFPCLDELLETHRHFFFSMKERRQESCAGSDRNFVINQIGDILVQQFSEENASKMKRIYGEFCSHHKEAMSLFKELQQNKKFQNFIKIRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKERTEEHRDLCKALGLIKDMIAAVDLKVSEYEKNQKWLEILNKIENKTYTKLKNGHVFRKQALLSQERALLHDGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNNWMRRIQQAVESCPEEEGGRTSESDEERRKAEARVAKIQQCQEILSNQDQQICTYLEEKLHIYAELGELSGFEDVHLEPHLLIKPDPGEPPQAASLLAAALREAESLQVAVKASKMGDVSQSSEESPGGTVLMDTPSTQDVPASPTASLVTEGTEGRGCWDVDPGLQGVVTDLAVSDAGEKVEYRSFSGSSQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIHKLVLQQRESLAPSHSFRGGPLQDQEKSRYLEKQREELANIHKLQHQFQQEQRRWHRTCDQQQREQEAQESWLQARERECQSQEELLLRHRSELDHQLQEYQQSLERLREGQRMVERERQKMRVQQGLLGHCKHSRQRSLPAVFSPGSKEVTELNRAESLCHENSFFINEAFGHMSLNTSNKPNPSGVPWDAHPLEGSHFDLARTSESPTELKIDISQPPDVNSELWTTGPGHQRPALQENSKESYKNVADLDSFQSESSSPQDSNQRGPQPQTLTTEAKLSLPMAAGHGGDAGDGAEENILYL

Target ORF information:

RefSeq Version	XM_017315361.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315361.2

ORF Insert Sequence:

ATGGGCTCTG GCTCAGTGAC CTACGTGGAC AACATGGCCT GCAGGCTGGC TCGTCTGCTG 
GTCACTCAGG CTGACCGACT CACGGCCTGC AGTCACCAGA CGCTGCTGAC CCCGTTTGCC 
CTGACAGTGG AAGCGCTGCC TGCCTTGGAT GAGGAGCTCG TGCTGGCTCT GACCCAACTG 
GAGCTGCCTT TGGGTTGGAC AGTCCTGGGA AATTCCTCAC TTGAAGTATC TCTACACAGA 
GAGTCTCTCC TGCATCTGGC TGTGAGATGG GCCCTCCCTA AGCTATTCCA CTTCCTCTTG 
TGTCTCCCTG GAGGAGTCAA GGCCTTGAAG CTACCTAATG AAGAGGCCAC CACACCATTA 
GACTTAGCTT TGCAAGGTGG ACACTCCACA CTGGTAGAAG ACATCACAAA TTTTCAGGGT 
AGCCATTCCC CAGGCTTCTC CCGACTGCGT CTTAACGAAG AGGCCACTCT GCAGTTTGTT 
CATTCATCGG AAACATTGAC CCTGACCGTT AACCACACAG CTGAGCATCT CTTGGAAGCA 
GACATCAAAC TCTTCCGGAA ATATTTCTGG GACAGAGCCT TTCTTGTCAA GGCTCTTGAG 
CAAGAAGCCA AGACTGAGAA AGCAACCATG CCTTCTGGAG CTGCAGAAAC TGAAGAAGAG 
GTTAGGAATT TGGAGTCTGG CAGATCACCA TCGGAGGAGG AGGAGGATGC AAAGAGCATC 
AAAAGCCAGG TGGATGGGCC GAGTGAACAC GAAGACCAGG ACCGCCTACC TCTGGATCGC 
TCCTTTGATG GCCTCAAAAA ATCCAAGCAT GTCCCAGCGT CCCTTGCTGC AGGCCAGCTC 
TCTGATGTTC TGAACGGGGG TGATGAAGTC TACGCTAACT GTATGGTGAT TGACCAGGTT 
GGCGATTTGG ATATCAACTA TATAAACCTA GAGGGACTCT CCACCCATAC GAGTCCCGAG 
TCTGGGAGGT CCATGCTGGG GCCTCAGGCT TGCATGCACA CCCTTCCTCC CGACACCAGT 
CCCTGCGGGC GCCCACTCAT TGAGAACAGT GAGGGGACCC TGGATGCTGC AGCTTCGCAG 
TCTTTCGTGA CACCATCCAG TTCTCGCACT TCCAACTTGA ACCTTTCTTT TGGTCTCCAT 
GGATTTGAAA AGGAGCAGAG TCATCTCAAG AAAAGGAGTT CTAGCCTGGA TGCCCTGGTT 
GCCGACAGTG AGGGGGAAGG AGGTTCTGAG CCCCCCATCT GCTATGCTGT GGGCTCTCAG 
AGTTCCCCAA GAACTGGACT CCCCAGCGGG GATGAACTGG ACTCTTTTGA GACCAACACT 
GAACCAGACT GTAATATCTC CAGGACCGAA TCACTCTCTC TGTCCAGTAC TCTGCATTCA 
AAGGAGTCCT TGCTTTCTGG GATCCGGTCT CGCTCCTACT CCTGCTCATC ACCCAAAATC 
TCTTCAGGAA AATCCCGGCT AGTGCGCGAC TTCACAGTGT GCAGTACCAG CGAAGAACAG 
AGATCATATA GTTTCCAGGA GCCACCAGGA GAAAAAAGGA TTCAGGAAGA AGAATGGGAT 
GAATACGTCA TTCCTGCCAA ATCCGAGTCT GAAAAGTACA AAGTGAGCCG AACTTTCAGC 
TTCCTCATGA ACAGGATGAC CAGCCCACGG AACAAATCTA AGATGAAAAA CAAGGACACT 
AAAGAAAAAG AAAAAATGAA TCGACATCAG TTTGTCCCGG GAACGTTCTC CGGGGTTCTG 
CAGTGCTCCG GTTGTGATAA AACACTCCTG GGGAAAGAGT CACTCCAGTG TGCCAACTGT 
AAGGCAAACA CACACAAGGG CTGCAAAGAT GCTGTGCCTC CGTGTACCAA GAAATTCCAA 
GAGAAATACA ACAAGAACAA ACCACAGAGC ATCCTTGGAA GCTCTTCAGT CAGAGACGTC 
CCAGCACCTG GGCTCTCCTT GCACCCTTCT TCCTCTATGC CCATCGGACT GCCAGCTGGA 
AGGAAGGAAT TTGCGGCTCA GGTGCATCCA CTGTCCAGAA GTGTCCCCGG CACCACTTTG 
GAAAGCTTCA GGAGGGCAGT GACATCGTTA GAGTCTGAGG GTGACAGCTG GAGAAGCAGG 
TCTCATTCCG ATGAGCTGTT CCAGTCTATG GGCTCTTCTC CTTCCACGGA GTCATTCATG 
ATGGAAGATG TTGTGGATTC TTCTCTGTGG ATCGACCTCA GTAGTGATGC TCAGGAGTTT 
GAAGCAGAGT CTTGGAGTCT CGTGGTGGAC CCTTCCTTTT GCAGTAGGCA GGAGAAGGAT 
GTCATCAAAA GACAAGATGT CATTTTTGAG CTGATGCAGA CAGAGGTCCA CCACATCCAG 
ACTCTGCTCA TCATGTCCGA GGTCTTCAGG AAAGGCATGA AGGAGGAACT GCAGCTGGAC 
CACAGCACGG TGGACAAGAT CTTCCCCTGC TTAGATGAGC TGCTGGAGAC TCACCGGCAC 
TTCTTCTTCA GCATGAAGGA GCGCAGGCAG GAGTCCTGCG CTGGCAGTGA CAGGAACTTT 
GTGATCAACC AAATTGGAGA CATTCTGGTA CAACAGTTTT CAGAGGAAAA TGCAAGCAAA 
ATGAAGAGGA TCTATGGAGA ATTCTGCAGC CATCACAAAG AAGCCATGAG TCTCTTTAAA 
GAGCTGCAGC AGAACAAGAA GTTTCAGAAT TTTATTAAGA TCCGAAATAG CAACCTGTTG 
GCTCGTCGCC GCGGAATCCC GGAGTGCATC CTGCTAGTTA CCCAGCGCAT CACAAAGTAC 
CCAGTCTTGG TGGAAAGGAT CCTGCAGTAC ACCAAGGAAA GGACTGAAGA GCACCGGGAC 
CTGTGCAAAG CTCTCGGCTT AATTAAAGAC ATGATCGCAG CCGTGGACCT GAAAGTCAGC 
GAATACGAGA AGAACCAAAA ATGGCTCGAG ATCCTAAATA AAATCGAGAA CAAAACATAC 
ACAAAGCTCA AAAATGGCCA CGTGTTTAGA AAGCAGGCGC TGCTGAGCCA GGAACGGGCT 
CTCCTGCATG ATGGGCTTGT CTACTGGAAA ACCGCTACGG GTCGCTTCAA AGACATCCTG 
GCTCTGCTAC TAACTGACGT GCTGCTCTTC TTACAAGAAA AAGACCAGAA ATACATCTTT 
GCAGCTGTTG ACCAGAAGCC ATCAGTGATT TCCCTCCAGA AGCTGATTGC TAGAGAAGTT 
GCCAACGAGG AGAGAGGCAT GTTTCTGATC AGTGCTTCGT CAGCCGGCCC CGAGATGTAC 
GAAATCCACA CCAATTCCAA GGAGGAGCGG AATAACTGGA TGAGGCGGAT CCAGCAGGCA 
GTAGAAAGTT GTCCAGAGGA AGAAGGAGGA AGGACGAGCG AGTCGGATGA AGAGAGGCGG 
AAAGCTGAAG CTAGAGTGGC CAAGATTCAG CAATGTCAAG AAATACTCAG TAACCAAGAC 
CAACAGATTT GCACGTACTT GGAAGAAAAG CTGCATATCT ACGCTGAACT TGGGGAACTG 
AGTGGCTTTG AGGATGTCCA CCTAGAACCC CATCTGCTCA TCAAACCTGA CCCTGGAGAG 
CCTCCCCAGG CAGCGTCCCT GCTTGCAGCA GCCCTGAGAG AAGCCGAGAG TCTGCAAGTG 
GCTGTGAAGG CTTCAAAGAT GGGCGATGTA AGCCAGTCAT CCGAAGAAAG CCCTGGAGGG 
ACTGTCCTGA TGGACACACC CAGTACACAG GATGTACCAG CGTCACCAAC TGCTTCATTA 
GTCACAGAAG GGACAGAAGG AAGAGGCTGT TGGGATGTGG ATCCCGGGCT CCAGGGTGTG 
GTAACCGACT TGGCAGTCTC TGATGCAGGG GAGAAGGTGG AATATAGAAG TTTCTCCGGT 
TCTTCACAGT CAGAGATTAT ACAAGCAATA CAGAATTTAA CGCGTCTCCT CTACAGCCTT 
CAGGCTGCCT TGACCATCCA GGACAGTCAC ATTGAGATCC ACAAACTGGT TCTACAGCAG 
CGGGAGAGCT TGGCCCCTAG CCACTCCTTC CGAGGTGGCC CCTTGCAAGA CCAGGAGAAA 
TCCCGCTACC TGGAAAAGCA GCGTGAAGAG CTGGCCAACA TCCACAAGCT TCAACACCAG 
TTCCAGCAGG AGCAGCGGCG CTGGCACCGC ACGTGCGACC AGCAACAGCG CGAGCAGGAA 
GCCCAAGAGA GCTGGCTGCA GGCGCGGGAA CGTGAGTGCC AGTCCCAGGA GGAATTGTTG 
CTGCGCCACC GTAGCGAGCT GGACCACCAA CTCCAGGAGT ACCAGCAGAG CCTTGAGCGG 
CTACGGGAGG GCCAGCGGAT GGTGGAGAGG GAGAGACAGA AGATGCGTGT CCAGCAGGGT 
CTGCTGGGCC ACTGCAAGCA TAGTCGCCAG CGGAGCCTCC CAGCCGTCTT CTCTCCAGGG 
AGCAAGGAGG TAACAGAACT TAACCGAGCT GAGAGTTTGT GTCATGAAAA TTCATTTTTC 
ATCAATGAAG CTTTTGGACA CATGTCACTT AACACGTCCA ATAAACCAAA CCCATCAGGG 
GTCCCCTGGG ATGCTCACCC CCTGGAGGGG TCTCATTTTG ATTTGGCAAG GACTAGTGAG 
AGTCCAACAG AACTGAAGAT AGACATTTCT CAGCCTCCTG ATGTCAACTC TGAACTCTGG 
ACAACGGGGC CGGGCCATCA GAGACCTGCT CTCCAAGAAA ACAGCAAGGA GTCTTATAAA 
AATGTTGCAG ATTTGGACTC CTTCCAGAGT GAATCGTCAT CTCCCCAGGA CTCCAATCAG 
CGTGGCCCCC AGCCACAGAC ACTCACAACA GAAGCAAAAC TAAGTCTTCC GATGGCGGCT 
GGACACGGTG GCGATGCTGG AGACGGGGCT GAAGAAAACA TTCTTTACCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgef28 ( NM_012026.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_036156.2 Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), mRNA.

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CloneID	OMu09358
Clone ID Related Accession (Same CDS sequence)	NM_012026.2
Accession Version	NM_012026.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	rho guanine nucleotide exchange factor 28
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY778560.1, BC098331.1, BC075734.1 and AC124397.5. On Jan 20, 2007 this sequence version replaced NM_012026.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U73199.1, BC098331.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGTTGA GCTGCAGTGA AGTGCCTCTT TATGGACAGA AGACGGTCTA TGCAAAGTTT 
GGCAAAAACG TGTATCTACC CGAAGATGCG GAGTTTTACT TTGTTTATGG CGGGTCGCAT 
CAGAGGCACG TGGTGATTGC TGACCGTGTC CAGGACAATG TCCTCCAATC TAGCATTCCA 
GGCCACTGGC TTCAGGAGAC GGTGACGGTA TCTGTGTGCC TCTGCTCGGA AGGGTACTCC 
CCAGTGACCA TGGGCTCTGG CTCAGTGACC TACGTGGACA ACATGGCCTG CAGGCTGGCT 
CGTCTGCTGG TCACTCAGGC TGACCGACTC ACGGCCTGCA GTCACCAGAC GCTGCTGACC 
CCGTTTGCCC TGACAGTGGA AGCGCTGCCT GCCTTGGATG AGGAGCTCGT GCTGGCTCTG 
ACCCAACTGG AGCTGCCTTT GGGTTGGACA GTCCTGGGAA ATTCCTCACT TGAAGTATCT 
CTACACAGAG AGTCTCTCCT GCATCTGGCT GTGAGATGGG CCCTCCCTAA GCTATTCCAC 
TTCCTCTTGT GTCTCCCTGG AGGAGTCAAG GCCTTGAAGC TACCTAATGA AGAGGCCACC 
ACACCATTAG ACTTAGCTTT GCAAGGTGGA CACTCCACAC TGGTAGAAGA CATCACAAAT 
TTTCAGGGTA GCCATTCCCC AGGCTTCTCC CGACTGCGTC TTAACGAAGA GGCCACTCTG 
CAGTTTGTTC ATTCATCGGA AACATTGACC CTGACCGTTA ACCACACAGC TGAGCATCTC 
TTGGAAGCAG ACATCAAACT CTTCCGGAAA TATTTCTGGG ACAGAGCCTT TCTTGTCAAG 
GCTCTTGAGC AAGAAGCCAA GACTGAGAAA GCAACCATGC CTTCTGGAGC TGCAGAAACT 
GAAGAAGAGG TTAGGAATTT GGAGTCTGGC AGATCACCAT CGGAGGAGGA GGAGGATGCA 
AAGAGCATCA AAAGCCAGGT GGATGGGCCG AGTGAACACG AAGACCAGGA CCGCCTACCT 
CTGGATCGCT CCTTTGATGG CCTCAAAAAA TCCAAGCATG TCCCAGCGTC CCTTGCTGCA 
GGCCAGCTCT CTGATGTTCT GAACGGGGGT GATGAAGTCT ACGCTAACTG TATGGTGATT 
GACCAGGTTG GCGATTTGGA TATCAACTAT ATAAACCTAG AGGGACTCTC CACCCATACG 
AGTCCCGAGT CTGGGAGGTC CATGCTGGGG CCTCAGGCTT GCATGCACAC CCTTCCTCCC 
GACACCAGTC CCTGCGGGCG CCCACTCATT GAGAACAGTG AGGGGACCCT GGATGCTGCA 
GCTTCGCAGT CTTTCGTGAC ACCATCCAGT TCTCGCACTT CCAACTTGAA CCTTTCTTTT 
GGTCTCCATG GATTTGAAAA GGAGCAGAGT CATCTCAAGA AAAGGAGTTC TAGCCTGGAT 
GCCCTGGTTG CCGACAGTGA GGGGGAAGGA GGTTCTGAGC CCCCCATCTG CTATGCTGTG 
GGCTCTCAGA GTTCCCCAAG AACTGGACTC CCCAGCGGGG ATGAACTGGA CTCTTTTGAG 
ACCAACACTG AACCAGACTG TAATATCTCC AGGACCGAAT CACTCTCTCT GTCCAGTACT 
CTGCATTCAA AGGAGTCCTT GCTTTCTGGG ATCCGGTCTC GCTCCTACTC CTGCTCATCA 
CCCAAAATCT CTTCAGGAAA ATCCCGGCTA GTGCGCGACT TCACAGTGTG CAGTACCAGC 
GAAGAACAGA GATCATATAG TTTCCAGGAG CCACCAGGAG AAAAAAGGAT TCAGGAAGAA 
GAATGGGATG AATACGTCAT TCCTGCCAAA TCCGAGTCTG AAAAGTACAA AGTGAGCCGA 
ACTTTCAGCT TCCTCATGAA CAGGATGACC AGCCCACGGA ACAAATCTAA GATGAAAAAC 
AAGGACACTA AAGAAAAAGA AAAAATGAAT CGACATCAGT TTGTCCCGGG AACGTTCTCC 
GGGGTTCTGC AGTGCTCCGG TTGTGATAAA ACACTCCTGG GGAAAGAGTC ACTCCAGTGT 
GCCAACTGTA AGGCAAACAC ACACAAGGGC TGCAAAGATG CTGTGCCTCC GTGTACCAAG 
AAATTCCAAG AGAAATACAA CAAGAACAAA CCACAGAGCA TCCTTGGAAG CTCTTCAGTC 
AGAGACGTCC CAGCACCTGG GCTCTCCTTG CACCCTTCTT CCTCTATGCC CATCGGACTG 
CCAGCTGGAA GGAAGGAATT TGCGGCTCAG GTGCATCCAC TGTCCAGAAG TGTCCCCGGC 
ACCACTTTGG AAAGCTTCAG GAGGGCAGTG ACATCGTTAG AGTCTGAGGG TGACAGCTGG 
AGAAGCAGGT CTCATTCCGA TGAGCTGTTC CAGTCTATGG GCTCTTCTCC TTCCACGGAG 
TCATTCATGA TGGAAGATGT TGTGGATTCT TCTCTGTGGA TCGACCTCAG TAGTGATGCT 
CAGGAGTTTG AAGCAGAGTC TTGGAGTCTC GTGGTGGACC CTTCCTTTTG CAGTAGGCAG 
GAGAAGGATG TCATCAAAAG ACAAGATGTC ATTTTTGAGC TGATGCAGAC AGAGGTCCAC 
CACATCCAGA CTCTGCTCAT CATGTCCGAG GTCTTCAGGA AAGGCATGAA GGAGGAACTG 
CAGCTGGACC ACAGCACGGT GGACAAGATC TTCCCCTGCT TAGATGAGCT GCTGGAGACT 
CACCGGCACT TCTTCTTCAG CATGAAGGAG CGCAGGCAGG AGTCCTGCGC TGGCAGTGAC 
AGGAACTTTG TGATCAACCA AATTGGAGAC ATTCTGGTAC AACAGTTTTC AGAGGAAAAT 
GCAAGCAAAA TGAAGAGGAT CTATGGAGAA TTCTGCAGCC ATCACAAAGA AGCCATGAGT 
CTCTTTAAAG AGCTGCAGCA GAACAAGAAG TTTCAGAATT TTATTAAGAT CCGAAATAGC 
AACCTGTTGG CTCGTCGCCG CGGAATCCCG GAGTGCATCC TGCTAGTTAC CCAGCGCATC 
ACAAAGTACC CAGTCTTGGT GGAAAGGATC CTGCAGTACA CCAAGGAAAG GACTGAAGAG 
CACCGGGACC TGTGCAAAGC TCTCGGCTTA ATTAAAGACA TGATCGCAGC CGTGGACCTG 
AAAGTCAGCG AATACGAGAA GAACCAAAAA TGGCTCGAGA TCCTAAATAA AATCGAGAAC 
AAAACATACA CAAAGCTCAA AAATGGCCAC GTGTTTAGAA AGCAGGCGCT GCTGAGCCAG 
GAACGGGCTC TCCTGCATGA TGGGCTTGTC TACTGGAAAA CCGCTACGGG TCGCTTCAAA 
GACATCCTGG CTCTGCTACT AACTGACGTG CTGCTCTTCT TACAAGAAAA AGACCAGAAA 
TACATCTTTG CAGCTGTTGA CCAGAAGCCA TCAGTGATTT CCCTCCAGAA GCTGATTGCT 
AGAGAAGTTG CCAACGAGGA GAGAGGCATG TTTCTGATCA GTGCTTCGTC AGCCGGCCCC 
GAGATGTACG AAATCCACAC CAATTCCAAG GAGGAGCGGA ATAACTGGAT GAGGCGGATC 
CAGCAGGCAG TAGAAAGTTG TCCAGAGGAA GAAGGAGGAA GGACGAGCGA GTCGGATGAA 
GAGAGGCGGA AAGCTGAAGC TAGAGTGGCC AAGATTCAGC AATGTCAAGA AATACTCAGT 
AACCAAGACC AACAGATTTG CACGTACTTG GAAGAAAAGC TGCATATCTA CGCTGAACTT 
GGGGAACTGA GTGGCTTTGA GGATGTCCAC CTAGAACCCC ATCTGCTCAT CAAACCTGAC 
CCTGGAGAGC CTCCCCAGGC AGCGTCCCTG CTTGCAGCAG CCCTGAGAGA AGCCGAGAGT 
CTGCAAGTGG CTGTGAAGGC TTCAAAGATG GGCGATGTAA GCCAGTCATC CGAAGAAAGC 
CCTGGAGGGA CTGTCCTGAT GGACACACCC AGTACACAGG ATGTACCAGC GTCACCAACT 
GCTTCATTAG TCACAGAAGG GACAGAAGGA AGAGGCTGTT GGGATGTGGA TCCCGGGCTC 
CAGGGTGTGG TAACCGACTT GGCAGTCTCT GATGCAGGGG AGAAGGTGGA ATATAGAAGT 
TTCTCCGGTT CTTCACAGTC AGAGATTATA CAAGCAATAC AGAATTTAAC GCGTCTCCTC 
TACAGCCTTC AGGCTGCCTT GACCATCCAG GACAGTCACA TTGAGATCCA CAAACTGGTT 
CTACAGCAGC GGGAGAGCTT GGCCCCTAGC CACTCCTTCC GAGGTGGCCC CTTGCAAGAC 
CAGGAGAAAT CCCGCTACCT GGAAAAGCAG CGTGAAGAGC TGGCCAACAT CCACAAGCTT 
CAACACCAGT TCCAGCAGGA GCAGCGGCGC TGGCACCGCA CGTGCGACCA GCAACAGCGC 
GAGCAGGAAG CCCAAGAGAG CTGGCTGCAG GCGCGGGAAC GTGAGTGCCA GTCCCAGGAG 
GAATTGTTGC TGCGCCACCG TAGCGAGCTG GACCACCAAC TCCAGGAGTA CCAGCAGAGC 
CTTGAGCGGC TACGGGAGGG CCAGCGGATG GTGGAGAGGG AGAGACAGAA GATGCGTGTC 
CAGCAGGGTC TGCTGGGCCA CTGCAAGCAT AGTCGCCAGC GGAGCCTCCC AGCCGTCTTC 
TCTCCAGGGA GCAAGGAGGT AACAGAACTT AACCGAGCTG AGAGTTTGTG TCATGAAAAT 
TCATTTTTCA TCAATGAAGC TTTTGGACAC ATGTCACTTA ACACGTCCAA TAAACCAAAC 
CCATCAGGGG TCCCCTGGGA TGCTCACCCC CTGGAGGGGT CTCATTTTGA TTTGGCAAGG 
ACTAGTGAGA GTCCAACAGA ACTGAAGATA GACATTTCTC AGCCTCCTGA TGTCAACTCT 
GAACTCTGGA CAACGGGGCC GGGCCATCAG AGACCTGCTC TCCAAGAAAA CAGCAAGGAG 
TCTTATAAAA ATGTTGCAGA TTTGGACTCC TTCCAGAGTG AATCGTCATC TCCCCAGGAC 
TCCAATCAGC GTGGCCCCCA GCCACAGACA CTCACAACAG AAGCAAAACT AAGTCTTCCG 
ATGGCGGCTG GACACGGTGG CGATGCTGGA GACGGGGCTG AAGAAAACAT TCTTTACCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036156.2
CDS	105..5207
Translation	MELSCSEVPLYGQKTVYAKFGKNVYLPEDAEFYFVYGGSHQRHVVIADRVQDNVLQSSIPGHWLQETVTVSVCLCSEGYSPVTMGSGSVTYVDNMACRLARLLVTQADRLTACSHQTLLTPFALTVEALPALDEELVLALTQLELPLGWTVLGNSSLEVSLHRESLLHLAVRWALPKLFHFLLCLPGGVKALKLPNEEATTPLDLALQGGHSTLVEDITNFQGSHSPGFSRLRLNEEATLQFVHSSETLTLTVNHTAEHLLEADIKLFRKYFWDRAFLVKALEQEAKTEKATMPSGAAETEEEVRNLESGRSPSEEEEDAKSIKSQVDGPSEHEDQDRLPLDRSFDGLKKSKHVPASLAAGQLSDVLNGGDEVYANCMVIDQVGDLDINYINLEGLSTHTSPESGRSMLGPQACMHTLPPDTSPCGRPLIENSEGTLDAAASQSFVTPSSSRTSNLNLSFGLHGFEKEQSHLKKRSSSLDALVADSEGEGGSEPPICYAVGSQSSPRTGLPSGDELDSFETNTEPDCNISRTESLSLSSTLHSKESLLSGIRSRSYSCSSPKISSGKSRLVRDFTVCSTSEEQRSYSFQEPPGEKRIQEEEWDEYVIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKMKNKDTKEKEKMNRHQFVPGTFSGVLQCSGCDKTLLGKESLQCANCKANTHKGCKDAVPPCTKKFQEKYNKNKPQSILGSSSVRDVPAPGLSLHPSSSMPIGLPAGRKEFAAQVHPLSRSVPGTTLESFRRAVTSLESEGDSWRSRSHSDELFQSMGSSPSTESFMMEDVVDSSLWIDLSSDAQEFEAESWSLVVDPSFCSRQEKDVIKRQDVIFELMQTEVHHIQTLLIMSEVFRKGMKEELQLDHSTVDKIFPCLDELLETHRHFFFSMKERRQESCAGSDRNFVINQIGDILVQQFSEENASKMKRIYGEFCSHHKEAMSLFKELQQNKKFQNFIKIRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKERTEEHRDLCKALGLIKDMIAAVDLKVSEYEKNQKWLEILNKIENKTYTKLKNGHVFRKQALLSQERALLHDGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNNWMRRIQQAVESCPEEEGGRTSESDEERRKAEARVAKIQQCQEILSNQDQQICTYLEEKLHIYAELGELSGFEDVHLEPHLLIKPDPGEPPQAASLLAAALREAESLQVAVKASKMGDVSQSSEESPGGTVLMDTPSTQDVPASPTASLVTEGTEGRGCWDVDPGLQGVVTDLAVSDAGEKVEYRSFSGSSQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIHKLVLQQRESLAPSHSFRGGPLQDQEKSRYLEKQREELANIHKLQHQFQQEQRRWHRTCDQQQREQEAQESWLQARERECQSQEELLLRHRSELDHQLQEYQQSLERLREGQRMVERERQKMRVQQGLLGHCKHSRQRSLPAVFSPGSKEVTELNRAESLCHENSFFINEAFGHMSLNTSNKPNPSGVPWDAHPLEGSHFDLARTSESPTELKIDISQPPDVNSELWTTGPGHQRPALQENSKESYKNVADLDSFQSESSSPQDSNQRGPQPQTLTTEAKLSLPMAAGHGGDAGDGAEENILYL

Target ORF information:

RefSeq Version	NM_012026.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012026.2

ORF Insert Sequence:

ATGGAGTTGA GCTGCAGTGA AGTGCCTCTT TATGGACAGA AGACGGTCTA TGCAAAGTTT 
GGCAAAAACG TGTATCTACC CGAAGATGCG GAGTTTTACT TTGTTTATGG CGGGTCGCAT 
CAGAGGCACG TGGTGATTGC TGACCGTGTC CAGGACAATG TCCTCCAATC TAGCATTCCA 
GGCCACTGGC TTCAGGAGAC GGTGACGGTA TCTGTGTGCC TCTGCTCGGA AGGGTACTCC 
CCAGTGACCA TGGGCTCTGG CTCAGTGACC TACGTGGACA ACATGGCCTG CAGGCTGGCT 
CGTCTGCTGG TCACTCAGGC TGACCGACTC ACGGCCTGCA GTCACCAGAC GCTGCTGACC 
CCGTTTGCCC TGACAGTGGA AGCGCTGCCT GCCTTGGATG AGGAGCTCGT GCTGGCTCTG 
ACCCAACTGG AGCTGCCTTT GGGTTGGACA GTCCTGGGAA ATTCCTCACT TGAAGTATCT 
CTACACAGAG AGTCTCTCCT GCATCTGGCT GTGAGATGGG CCCTCCCTAA GCTATTCCAC 
TTCCTCTTGT GTCTCCCTGG AGGAGTCAAG GCCTTGAAGC TACCTAATGA AGAGGCCACC 
ACACCATTAG ACTTAGCTTT GCAAGGTGGA CACTCCACAC TGGTAGAAGA CATCACAAAT 
TTTCAGGGTA GCCATTCCCC AGGCTTCTCC CGACTGCGTC TTAACGAAGA GGCCACTCTG 
CAGTTTGTTC ATTCATCGGA AACATTGACC CTGACCGTTA ACCACACAGC TGAGCATCTC 
TTGGAAGCAG ACATCAAACT CTTCCGGAAA TATTTCTGGG ACAGAGCCTT TCTTGTCAAG 
GCTCTTGAGC AAGAAGCCAA GACTGAGAAA GCAACCATGC CTTCTGGAGC TGCAGAAACT 
GAAGAAGAGG TTAGGAATTT GGAGTCTGGC AGATCACCAT CGGAGGAGGA GGAGGATGCA 
AAGAGCATCA AAAGCCAGGT GGATGGGCCG AGTGAACACG AAGACCAGGA CCGCCTACCT 
CTGGATCGCT CCTTTGATGG CCTCAAAAAA TCCAAGCATG TCCCAGCGTC CCTTGCTGCA 
GGCCAGCTCT CTGATGTTCT GAACGGGGGT GATGAAGTCT ACGCTAACTG TATGGTGATT 
GACCAGGTTG GCGATTTGGA TATCAACTAT ATAAACCTAG AGGGACTCTC CACCCATACG 
AGTCCCGAGT CTGGGAGGTC CATGCTGGGG CCTCAGGCTT GCATGCACAC CCTTCCTCCC 
GACACCAGTC CCTGCGGGCG CCCACTCATT GAGAACAGTG AGGGGACCCT GGATGCTGCA 
GCTTCGCAGT CTTTCGTGAC ACCATCCAGT TCTCGCACTT CCAACTTGAA CCTTTCTTTT 
GGTCTCCATG GATTTGAAAA GGAGCAGAGT CATCTCAAGA AAAGGAGTTC TAGCCTGGAT 
GCCCTGGTTG CCGACAGTGA GGGGGAAGGA GGTTCTGAGC CCCCCATCTG CTATGCTGTG 
GGCTCTCAGA GTTCCCCAAG AACTGGACTC CCCAGCGGGG ATGAACTGGA CTCTTTTGAG 
ACCAACACTG AACCAGACTG TAATATCTCC AGGACCGAAT CACTCTCTCT GTCCAGTACT 
CTGCATTCAA AGGAGTCCTT GCTTTCTGGG ATCCGGTCTC GCTCCTACTC CTGCTCATCA 
CCCAAAATCT CTTCAGGAAA ATCCCGGCTA GTGCGCGACT TCACAGTGTG CAGTACCAGC 
GAAGAACAGA GATCATATAG TTTCCAGGAG CCACCAGGAG AAAAAAGGAT TCAGGAAGAA 
GAATGGGATG AATACGTCAT TCCTGCCAAA TCCGAGTCTG AAAAGTACAA AGTGAGCCGA 
ACTTTCAGCT TCCTCATGAA CAGGATGACC AGCCCACGGA ACAAATCTAA GATGAAAAAC 
AAGGACACTA AAGAAAAAGA AAAAATGAAT CGACATCAGT TTGTCCCGGG AACGTTCTCC 
GGGGTTCTGC AGTGCTCCGG TTGTGATAAA ACACTCCTGG GGAAAGAGTC ACTCCAGTGT 
GCCAACTGTA AGGCAAACAC ACACAAGGGC TGCAAAGATG CTGTGCCTCC GTGTACCAAG 
AAATTCCAAG AGAAATACAA CAAGAACAAA CCACAGAGCA TCCTTGGAAG CTCTTCAGTC 
AGAGACGTCC CAGCACCTGG GCTCTCCTTG CACCCTTCTT CCTCTATGCC CATCGGACTG 
CCAGCTGGAA GGAAGGAATT TGCGGCTCAG GTGCATCCAC TGTCCAGAAG TGTCCCCGGC 
ACCACTTTGG AAAGCTTCAG GAGGGCAGTG ACATCGTTAG AGTCTGAGGG TGACAGCTGG 
AGAAGCAGGT CTCATTCCGA TGAGCTGTTC CAGTCTATGG GCTCTTCTCC TTCCACGGAG 
TCATTCATGA TGGAAGATGT TGTGGATTCT TCTCTGTGGA TCGACCTCAG TAGTGATGCT 
CAGGAGTTTG AAGCAGAGTC TTGGAGTCTC GTGGTGGACC CTTCCTTTTG CAGTAGGCAG 
GAGAAGGATG TCATCAAAAG ACAAGATGTC ATTTTTGAGC TGATGCAGAC AGAGGTCCAC 
CACATCCAGA CTCTGCTCAT CATGTCCGAG GTCTTCAGGA AAGGCATGAA GGAGGAACTG 
CAGCTGGACC ACAGCACGGT GGACAAGATC TTCCCCTGCT TAGATGAGCT GCTGGAGACT 
CACCGGCACT TCTTCTTCAG CATGAAGGAG CGCAGGCAGG AGTCCTGCGC TGGCAGTGAC 
AGGAACTTTG TGATCAACCA AATTGGAGAC ATTCTGGTAC AACAGTTTTC AGAGGAAAAT 
GCAAGCAAAA TGAAGAGGAT CTATGGAGAA TTCTGCAGCC ATCACAAAGA AGCCATGAGT 
CTCTTTAAAG AGCTGCAGCA GAACAAGAAG TTTCAGAATT TTATTAAGAT CCGAAATAGC 
AACCTGTTGG CTCGTCGCCG CGGAATCCCG GAGTGCATCC TGCTAGTTAC CCAGCGCATC 
ACAAAGTACC CAGTCTTGGT GGAAAGGATC CTGCAGTACA CCAAGGAAAG GACTGAAGAG 
CACCGGGACC TGTGCAAAGC TCTCGGCTTA ATTAAAGACA TGATCGCAGC CGTGGACCTG 
AAAGTCAGCG AATACGAGAA GAACCAAAAA TGGCTCGAGA TCCTAAATAA AATCGAGAAC 
AAAACATACA CAAAGCTCAA AAATGGCCAC GTGTTTAGAA AGCAGGCGCT GCTGAGCCAG 
GAACGGGCTC TCCTGCATGA TGGGCTTGTC TACTGGAAAA CCGCTACGGG TCGCTTCAAA 
GACATCCTGG CTCTGCTACT AACTGACGTG CTGCTCTTCT TACAAGAAAA AGACCAGAAA 
TACATCTTTG CAGCTGTTGA CCAGAAGCCA TCAGTGATTT CCCTCCAGAA GCTGATTGCT 
AGAGAAGTTG CCAACGAGGA GAGAGGCATG TTTCTGATCA GTGCTTCGTC AGCCGGCCCC 
GAGATGTACG AAATCCACAC CAATTCCAAG GAGGAGCGGA ATAACTGGAT GAGGCGGATC 
CAGCAGGCAG TAGAAAGTTG TCCAGAGGAA GAAGGAGGAA GGACGAGCGA GTCGGATGAA 
GAGAGGCGGA AAGCTGAAGC TAGAGTGGCC AAGATTCAGC AATGTCAAGA AATACTCAGT 
AACCAAGACC AACAGATTTG CACGTACTTG GAAGAAAAGC TGCATATCTA CGCTGAACTT 
GGGGAACTGA GTGGCTTTGA GGATGTCCAC CTAGAACCCC ATCTGCTCAT CAAACCTGAC 
CCTGGAGAGC CTCCCCAGGC AGCGTCCCTG CTTGCAGCAG CCCTGAGAGA AGCCGAGAGT 
CTGCAAGTGG CTGTGAAGGC TTCAAAGATG GGCGATGTAA GCCAGTCATC CGAAGAAAGC 
CCTGGAGGGA CTGTCCTGAT GGACACACCC AGTACACAGG ATGTACCAGC GTCACCAACT 
GCTTCATTAG TCACAGAAGG GACAGAAGGA AGAGGCTGTT GGGATGTGGA TCCCGGGCTC 
CAGGGTGTGG TAACCGACTT GGCAGTCTCT GATGCAGGGG AGAAGGTGGA ATATAGAAGT 
TTCTCCGGTT CTTCACAGTC AGAGATTATA CAAGCAATAC AGAATTTAAC GCGTCTCCTC 
TACAGCCTTC AGGCTGCCTT GACCATCCAG GACAGTCACA TTGAGATCCA CAAACTGGTT 
CTACAGCAGC GGGAGAGCTT GGCCCCTAGC CACTCCTTCC GAGGTGGCCC CTTGCAAGAC 
CAGGAGAAAT CCCGCTACCT GGAAAAGCAG CGTGAAGAGC TGGCCAACAT CCACAAGCTT 
CAACACCAGT TCCAGCAGGA GCAGCGGCGC TGGCACCGCA CGTGCGACCA GCAACAGCGC 
GAGCAGGAAG CCCAAGAGAG CTGGCTGCAG GCGCGGGAAC GTGAGTGCCA GTCCCAGGAG 
GAATTGTTGC TGCGCCACCG TAGCGAGCTG GACCACCAAC TCCAGGAGTA CCAGCAGAGC 
CTTGAGCGGC TACGGGAGGG CCAGCGGATG GTGGAGAGGG AGAGACAGAA GATGCGTGTC 
CAGCAGGGTC TGCTGGGCCA CTGCAAGCAT AGTCGCCAGC GGAGCCTCCC AGCCGTCTTC 
TCTCCAGGGA GCAAGGAGGT AACAGAACTT AACCGAGCTG AGAGTTTGTG TCATGAAAAT 
TCATTTTTCA TCAATGAAGC TTTTGGACAC ATGTCACTTA ACACGTCCAA TAAACCAAAC 
CCATCAGGGG TCCCCTGGGA TGCTCACCCC CTGGAGGGGT CTCATTTTGA TTTGGCAAGG 
ACTAGTGAGA GTCCAACAGA ACTGAAGATA GACATTTCTC AGCCTCCTGA TGTCAACTCT 
GAACTCTGGA CAACGGGGCC GGGCCATCAG AGACCTGCTC TCCAAGAAAA CAGCAAGGAG 
TCTTATAAAA ATGTTGCAGA TTTGGACTCC TTCCAGAGTG AATCGTCATC TCCCCAGGAC 
TCCAATCAGC GTGGCCCCCA GCCACAGACA CTCACAACAG AAGCAAAACT AAGTCTTCCG 
ATGGCGGCTG GACACGGTGG CGATGCTGGA GACGGGGCTG AAGAAAACAT TCTTTACCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgef28 ( XM_006517518.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517581.1 Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X1, mRNA.

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CloneID	OMu48946
Clone ID Related Accession (Same CDS sequence)	XM_006517518.2 , XM_006517518.3
Accession Version	XM_006517518.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	rho guanine nucleotide exchange factor 28 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006517518.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCGGC GACGAGCCCG GCTGGCCAGA GATGCAGGAG CCATGGAGTT GAGCTGCAGT 
GAAGTGCCTC TTTATGGACA GAAGACGGTC TATGCAAAGT TTGGCAAAAA CGTGTATCTA 
CCCGAAGATG CGGAGTTTTA CTTTGTTTAT GGCGGGTCGC ATCAGAGGCA CGTGGTGATT 
GCTGACCGTG TCCAGGACAA TGTCCTCCAA TCTAGCATTC CAGGCCACTG GCTTCAGGAG 
ACGGTGACGG TATCTGTGTG CCTCTGCTCG GAAGGGTACT CCCCAGTGAC CATGGGCTCT 
GGCTCAGTGA CCTACGTGGA CAACATGGCC TGCAGGCTGG CTCGTCTGCT GGTCACTCAG 
GCTGACCGAC TCACGGCCTG CAGTCACCAG ACGCTGCTGA CCCCGTTTGC CCTGACAGTG 
GAAGCGCTGC CTGCCTTGGA TGAGGAGCTC GTGCTGGCTC TGACCCAACT GGAGCTGCCT 
TTGGGTTGGA CAGTCCTGGG AAATTCCTCA CTTGAAGTAT CTCTACACAG AGAGTCTCTC 
CTGCATCTGG CTGTGAGATG GGCCCTCCCT AAGCTATTCC ACTTCCTCTT GTGTCTCCCT 
GGAGGAGTCA AGGCCTTGAA GCTACCTAAT GAAGAGGCCA CCACACCATT AGACTTAGCT 
TTGCAAGGTG GACACTCCAC ACTGGTAGAA GACATCACAA ATTTTCAGGG TAGCCATTCC 
CCAGGCTTCT CCCGACTGCG TCTTAACGAA GAGGCCACTC TGCAGTTTGT TCATTCATCG 
GAAACATTGA CCCTGACCGT TAACCACACA GCTGAGCATC TCTTGGAAGC AGACATCAAA 
CTCTTCCGGA AATATTTCTG GGACAGAGCC TTTCTTGTCA AGGCTCTTGA GCAAGAAGCC 
AAGACTGAGA AAGCAACCAT GCCTTCTGGA GCTGCAGAAA CTGAAGAAGA GGTTAGGAAT 
TTGGAGTCTG GCAGATCACC ATCGGAGGAG GAGGAGGATG CAAAGAGCAT CAAAAGCCAG 
GTGGATGGGC CGAGTGAACA CGAAGACCAG GACCGCCTAC CTCTGGATCG CTCCTTTGAT 
GGCCTCAAAA AATCCAAGCA TGTCCCAGCG TCCCTTGCTG CAGGCCAGCT CTCTGATGTT 
CTGAACGGGG GTGATGAAGT CTACGCTAAC TGTATGGTGA TTGACCAGGT TGGCGATTTG 
GATATCAACT ATATAAACCT AGAGGGACTC TCCACCCATA CGAGTCCCGA GTCTGGGAGG 
TCCATGCTGG GGCCTCAGGC TTGCATGCAC ACCCTTCCTC CCGACACCAG TCCCTGCGGG 
CGCCCACTCA TTGAGAACAG TGAGGGGACC CTGGATGCTG CAGCTTCGCA GTCTTTCGTG 
ACACCATCCA GTTCTCGCAC TTCCAACTTG AACCTTTCTT TTGGTCTCCA TGGATTTGAA 
AAGGAGCAGA GTCATCTCAA GAAAAGGAGT TCTAGCCTGG ATGCCCTGGT TGCCGACAGT 
GAGGGGGAAG GAGGTTCTGA GCCCCCCATC TGCTATGCTG TGGGCTCTCA GAGTTCCCCA 
AGAACTGGAC TCCCCAGCGG GGATGAACTG GACTCTTTTG AGACCAACAC TGAACCAGAC 
TGTAATATCT CCAGGACCGA ATCACTCTCT CTGTCCAGTA CTCTGCATTC AAAGGAGTCC 
TTGCTTTCTG GGATCCGGTC TCGCTCCTAC TCCTGCTCAT CACCCAAAAT CTCTTCAGGA 
AAATCCCGGC TAGTGCGCGA CTTCACAGTG TGCAGTACCA GCGAAGAACA GAGATCATAT 
AGTTTCCAGG AGCCACCAGG AGAAAAAAGG ATTCAGGAAG AAGAATGGGA TGAATACGTC 
ATTCCTGCCA AATCCGAGTC TGAAAAGTAC AAAGTGAGCC GAACTTTCAG CTTCCTCATG 
AACAGGATGA CCAGCCCACG GAACAAATCT AAGATGAAAA ACAAGGACAC TAAAGAAAAA 
GAAAAAATGA ATCGACATCA GTTTGTCCCG GGAACGTTCT CCGGGGTTCT GCAGTGCTCC 
GGTTGTGATA AAACACTCCT GGGGAAAGAG TCACTCCAGT GTGCCAACTG TAAGGCAAAC 
ACACACAAGG GCTGCAAAGA TGCTGTGCCT CCGTGTACCA AGAAATTCCA AGAGAAATAC 
AACAAGAACA AACCACAGAG CATCCTTGGA AGCTCTTCAG TCAGAGACGT CCCAGCACCT 
GGGCTCTCCT TGCACCCTTC TTCCTCTATG CCCATCGGAC TGCCAGCTGG AAGGAAGGAA 
TTTGCGGCTC AGGTGCATCC ACTGTCCAGA AGTGTCCCCG GCACCACTTT GGAAAGCTTC 
AGGAGGGCAG TGACATCGTT AGAGTCTGAG GGTGACAGCT GGAGAAGCAG GTCTCATTCC 
GATGAGCTGT TCCAGTCTAT GGGCTCTTCT CCTTCCACGG AGTCATTCAT GATGGAAGAT 
GTTGTGGATT CTTCTCTGTG GATCGACCTC AGTAGTGATG CTCAGGAGTT TGAAGCAGAG 
TCTTGGAGTC TCGTGGTGGA CCCTTCCTTT TGCAGTAGGC AGGAGAAGGA TGTCATCAAA 
AGACAAGATG TCATTTTTGA GCTGATGCAG ACAGAGGTCC ACCACATCCA GACTCTGCTC 
ATCATGTCCG AGGTCTTCAG GAAAGGCATG AAGGAGGAAC TGCAGCTGGA CCACAGCACG 
GTGGACAAGA TCTTCCCCTG CTTAGATGAG CTGCTGGAGA CTCACCGGCA CTTCTTCTTC 
AGCATGAAGG AGCGCAGGCA GGAGTCCTGC GCTGGCAGTG ACAGGAACTT TGTGATCAAC 
CAAATTGGAG ACATTCTGGT ACAACAGTTT TCAGAGGAAA ATGCAAGCAA AATGAAGAGG 
ATCTATGGAG AATTCTGCAG CCATCACAAA GAAGCCATGA GTCTCTTTAA AGAGCTGCAG 
CAGAACAAGA AGTTTCAGAA TTTTATTAAG ATCCGAAATA GCAACCTGTT GGCTCGTCGC 
CGCGGAATCC CGGAGTGCAT CCTGCTAGTT ACCCAGCGCA TCACAAAGTA CCCAGTCTTG 
GTGGAAAGGA TCCTGCAGTA CACCAAGGAA AGGACTGAAG AGCACCGGGA CCTGTGCAAA 
GCTCTCGGCT TAATTAAAGA CATGATCGCA GCCGTGGACC TGAAAGTCAG CGAATACGAG 
AAGAACCAAA AATGGCTCGA GATCCTAAAT AAAATCGAGA ACAAAACATA CACAAAGCTC 
AAAAATGGCC ACGTGTTTAG AAAGCAGGCG CTGCTGAGCC AGGAACGGGC TCTCCTGCAT 
GATGGGCTTG TCTACTGGAA AACCGCTACG GGTCGCTTCA AAGACATCCT GGCTCTGCTA 
CTAACTGACG TGCTGCTCTT CTTACAAGAA AAAGACCAGA AATACATCTT TGCAGCTGTT 
GACCAGAAGC CATCAGTGAT TTCCCTCCAG AAGCTGATTG CTAGAGAAGT TGCCAACGAG 
GAGAGAGGCA TGTTTCTGAT CAGTGCTTCG TCAGCCGGCC CCGAGATGTA CGAAATCCAC 
ACCAATTCCA AGGAGGAGCG GAATAACTGG ATGAGGCGGA TCCAGCAGGC AGTAGAAAGT 
TGTCCAGAGG AAGAAGGAGG AAGGACGAGC GAGTCGGATG AAGAGAGGCG GAAAGCTGAA 
GCTAGAGTGG CCAAGATTCA GCAATGTCAA GAAATACTCA GTAACCAAGA CCAACAGATT 
TGCACGTACT TGGAAGAAAA GCTGCATATC TACGCTGAAC TTGGGGAACT GAGTGGCTTT 
GAGGATGTCC ACCTAGAACC CCATCTGCTC ATCAAACCTG ACCCTGGAGA GCCTCCCCAG 
GCAGCGTCCC TGCTTGCAGC AGCCCTGAGA GAAGCCGAGA GTCTGCAAGT GGCTGTGAAG 
GCTTCAAAGA TGGGCGATGT AAGCCAGTCA TCCGAAGAAA GCCCTGGAGG GACTGTCCTG 
ATGGACACAC CCAGTACACA GGATGTACCA GCGTCACCAA CTGCTTCATT AGTCACAGAA 
GGGACAGAAG GAAGAGGCTG TTGGGATGTG GATCCCGGGC TCCAGGGTGT GGTAACCGAC 
TTGGCAGTCT CTGATGCAGG GGAGAAGGTG GAATATAGAA GTTTCTCCGG TTCTTCACAG 
TCAGAGATTA TACAAGCAAT ACAGAATTTA ACGCGTCTCC TCTACAGCCT TCAGGCTGCC 
TTGACCATCC AGGACAGTCA CATTGAGATC CACAAACTGG TTCTACAGCA GCGGGAGAGC 
TTGGCCCCTA GCCACTCCTT CCGAGGTGGC CCCTTGCAAG ACCAGGAGAA ATCCCGCTAC 
CTGGAAAAGC AGCGTGAAGA GCTGGCCAAC ATCCACAAGC TTCAACACCA GTTCCAGCAG 
GAGCAGCGGC GCTGGCACCG CACGTGCGAC CAGCAACAGC GCGAGCAGGA AGCCCAAGAG 
AGCTGGCTGC AGGCGCGGGA ACGTGAGTGC CAGTCCCAGG AGGAATTGTT GCTGCGCCAC 
CGTAGCGAGC TGGACCACCA ACTCCAGGAG TACCAGCAGA GCCTTGAGCG GCTACGGGAG 
GGCCAGCGGA TGGTGGAGAG GGAGAGACAG AAGATGCGTG TCCAGCAGGG TCTGCTGGGC 
CACTGCAAGC ATAGTCGCCA GCGGAGCCTC CCAGCCGTCT TCTCTCCAGG GAGCAAGGAG 
GTAACAGAAC TTAACCGAGC TGAGAGTTTG TGTCATGAAA ATTCATTTTT CATCAATGAA 
GCTTTTGGAC ACATGTCACT TAACACGTCC AATAAACCAA ACCCATCAGG GGTCCCCTGG 
GATGCTCACC CCCTGGAGGG GTCTCATTTT GATTTGGCAA GGACTAGTGA GAGTCCAACA 
GAACTGAAGA TAGACATTTC TCAGCCTCCT GATGTCAACT CTGAACTCTG GACAACGGGG 
CCGGGCCATC AGAGACCTGC TCTCCAAGAA AACAGCAAGG AGTCTTATAA AAATGTTGCA 
GATTTGGACT CCTTCCAGAG TGAATCGTCA TCTCCCCAGG ACTCCAATCA GCGTGGCCCC 
CAGCCACAGA CACTCACAAC AGAAGCAAAA CTAAGTCTTC CGATGGCGGC TGGACACGGT 
GGCGATGCTG GAGACGGGGC TGAAGAAAAC ATTCTTTACC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517581.1
CDS	261..5405
Translation	MRRRRARLARDAGAMELSCSEVPLYGQKTVYAKFGKNVYLPEDAEFYFVYGGSHQRHVVIADRVQDNVLQSSIPGHWLQETVTVSVCLCSEGYSPVTMGSGSVTYVDNMACRLARLLVTQADRLTACSHQTLLTPFALTVEALPALDEELVLALTQLELPLGWTVLGNSSLEVSLHRESLLHLAVRWALPKLFHFLLCLPGGVKALKLPNEEATTPLDLALQGGHSTLVEDITNFQGSHSPGFSRLRLNEEATLQFVHSSETLTLTVNHTAEHLLEADIKLFRKYFWDRAFLVKALEQEAKTEKATMPSGAAETEEEVRNLESGRSPSEEEEDAKSIKSQVDGPSEHEDQDRLPLDRSFDGLKKSKHVPASLAAGQLSDVLNGGDEVYANCMVIDQVGDLDINYINLEGLSTHTSPESGRSMLGPQACMHTLPPDTSPCGRPLIENSEGTLDAAASQSFVTPSSSRTSNLNLSFGLHGFEKEQSHLKKRSSSLDALVADSEGEGGSEPPICYAVGSQSSPRTGLPSGDELDSFETNTEPDCNISRTESLSLSSTLHSKESLLSGIRSRSYSCSSPKISSGKSRLVRDFTVCSTSEEQRSYSFQEPPGEKRIQEEEWDEYVIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKMKNKDTKEKEKMNRHQFVPGTFSGVLQCSGCDKTLLGKESLQCANCKANTHKGCKDAVPPCTKKFQEKYNKNKPQSILGSSSVRDVPAPGLSLHPSSSMPIGLPAGRKEFAAQVHPLSRSVPGTTLESFRRAVTSLESEGDSWRSRSHSDELFQSMGSSPSTESFMMEDVVDSSLWIDLSSDAQEFEAESWSLVVDPSFCSRQEKDVIKRQDVIFELMQTEVHHIQTLLIMSEVFRKGMKEELQLDHSTVDKIFPCLDELLETHRHFFFSMKERRQESCAGSDRNFVINQIGDILVQQFSEENASKMKRIYGEFCSHHKEAMSLFKELQQNKKFQNFIKIRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKERTEEHRDLCKALGLIKDMIAAVDLKVSEYEKNQKWLEILNKIENKTYTKLKNGHVFRKQALLSQERALLHDGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNNWMRRIQQAVESCPEEEGGRTSESDEERRKAEARVAKIQQCQEILSNQDQQICTYLEEKLHIYAELGELSGFEDVHLEPHLLIKPDPGEPPQAASLLAAALREAESLQVAVKASKMGDVSQSSEESPGGTVLMDTPSTQDVPASPTASLVTEGTEGRGCWDVDPGLQGVVTDLAVSDAGEKVEYRSFSGSSQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIHKLVLQQRESLAPSHSFRGGPLQDQEKSRYLEKQREELANIHKLQHQFQQEQRRWHRTCDQQQREQEAQESWLQARERECQSQEELLLRHRSELDHQLQEYQQSLERLREGQRMVERERQKMRVQQGLLGHCKHSRQRSLPAVFSPGSKEVTELNRAESLCHENSFFINEAFGHMSLNTSNKPNPSGVPWDAHPLEGSHFDLARTSESPTELKIDISQPPDVNSELWTTGPGHQRPALQENSKESYKNVADLDSFQSESSSPQDSNQRGPQPQTLTTEAKLSLPMAAGHGGDAGDGAEENILYL

Target ORF information:

RefSeq Version	XM_006517518.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006517518.2

ORF Insert Sequence:

ATGAGGCGGC GACGAGCCCG GCTGGCCAGA GATGCAGGAG CCATGGAGTT GAGCTGCAGT 
GAAGTGCCTC TTTATGGACA GAAGACGGTC TATGCAAAGT TTGGCAAAAA CGTGTATCTA 
CCCGAAGATG CGGAGTTTTA CTTTGTTTAT GGCGGGTCGC ATCAGAGGCA CGTGGTGATT 
GCTGACCGTG TCCAGGACAA TGTCCTCCAA TCTAGCATTC CAGGCCACTG GCTTCAGGAG 
ACGGTGACGG TATCTGTGTG CCTCTGCTCG GAAGGGTACT CCCCAGTGAC CATGGGCTCT 
GGCTCAGTGA CCTACGTGGA CAACATGGCC TGCAGGCTGG CTCGTCTGCT GGTCACTCAG 
GCTGACCGAC TCACGGCCTG CAGTCACCAG ACGCTGCTGA CCCCGTTTGC CCTGACAGTG 
GAAGCGCTGC CTGCCTTGGA TGAGGAGCTC GTGCTGGCTC TGACCCAACT GGAGCTGCCT 
TTGGGTTGGA CAGTCCTGGG AAATTCCTCA CTTGAAGTAT CTCTACACAG AGAGTCTCTC 
CTGCATCTGG CTGTGAGATG GGCCCTCCCT AAGCTATTCC ACTTCCTCTT GTGTCTCCCT 
GGAGGAGTCA AGGCCTTGAA GCTACCTAAT GAAGAGGCCA CCACACCATT AGACTTAGCT 
TTGCAAGGTG GACACTCCAC ACTGGTAGAA GACATCACAA ATTTTCAGGG TAGCCATTCC 
CCAGGCTTCT CCCGACTGCG TCTTAACGAA GAGGCCACTC TGCAGTTTGT TCATTCATCG 
GAAACATTGA CCCTGACCGT TAACCACACA GCTGAGCATC TCTTGGAAGC AGACATCAAA 
CTCTTCCGGA AATATTTCTG GGACAGAGCC TTTCTTGTCA AGGCTCTTGA GCAAGAAGCC 
AAGACTGAGA AAGCAACCAT GCCTTCTGGA GCTGCAGAAA CTGAAGAAGA GGTTAGGAAT 
TTGGAGTCTG GCAGATCACC ATCGGAGGAG GAGGAGGATG CAAAGAGCAT CAAAAGCCAG 
GTGGATGGGC CGAGTGAACA CGAAGACCAG GACCGCCTAC CTCTGGATCG CTCCTTTGAT 
GGCCTCAAAA AATCCAAGCA TGTCCCAGCG TCCCTTGCTG CAGGCCAGCT CTCTGATGTT 
CTGAACGGGG GTGATGAAGT CTACGCTAAC TGTATGGTGA TTGACCAGGT TGGCGATTTG 
GATATCAACT ATATAAACCT AGAGGGACTC TCCACCCATA CGAGTCCCGA GTCTGGGAGG 
TCCATGCTGG GGCCTCAGGC TTGCATGCAC ACCCTTCCTC CCGACACCAG TCCCTGCGGG 
CGCCCACTCA TTGAGAACAG TGAGGGGACC CTGGATGCTG CAGCTTCGCA GTCTTTCGTG 
ACACCATCCA GTTCTCGCAC TTCCAACTTG AACCTTTCTT TTGGTCTCCA TGGATTTGAA 
AAGGAGCAGA GTCATCTCAA GAAAAGGAGT TCTAGCCTGG ATGCCCTGGT TGCCGACAGT 
GAGGGGGAAG GAGGTTCTGA GCCCCCCATC TGCTATGCTG TGGGCTCTCA GAGTTCCCCA 
AGAACTGGAC TCCCCAGCGG GGATGAACTG GACTCTTTTG AGACCAACAC TGAACCAGAC 
TGTAATATCT CCAGGACCGA ATCACTCTCT CTGTCCAGTA CTCTGCATTC AAAGGAGTCC 
TTGCTTTCTG GGATCCGGTC TCGCTCCTAC TCCTGCTCAT CACCCAAAAT CTCTTCAGGA 
AAATCCCGGC TAGTGCGCGA CTTCACAGTG TGCAGTACCA GCGAAGAACA GAGATCATAT 
AGTTTCCAGG AGCCACCAGG AGAAAAAAGG ATTCAGGAAG AAGAATGGGA TGAATACGTC 
ATTCCTGCCA AATCCGAGTC TGAAAAGTAC AAAGTGAGCC GAACTTTCAG CTTCCTCATG 
AACAGGATGA CCAGCCCACG GAACAAATCT AAGATGAAAA ACAAGGACAC TAAAGAAAAA 
GAAAAAATGA ATCGACATCA GTTTGTCCCG GGAACGTTCT CCGGGGTTCT GCAGTGCTCC 
GGTTGTGATA AAACACTCCT GGGGAAAGAG TCACTCCAGT GTGCCAACTG TAAGGCAAAC 
ACACACAAGG GCTGCAAAGA TGCTGTGCCT CCGTGTACCA AGAAATTCCA AGAGAAATAC 
AACAAGAACA AACCACAGAG CATCCTTGGA AGCTCTTCAG TCAGAGACGT CCCAGCACCT 
GGGCTCTCCT TGCACCCTTC TTCCTCTATG CCCATCGGAC TGCCAGCTGG AAGGAAGGAA 
TTTGCGGCTC AGGTGCATCC ACTGTCCAGA AGTGTCCCCG GCACCACTTT GGAAAGCTTC 
AGGAGGGCAG TGACATCGTT AGAGTCTGAG GGTGACAGCT GGAGAAGCAG GTCTCATTCC 
GATGAGCTGT TCCAGTCTAT GGGCTCTTCT CCTTCCACGG AGTCATTCAT GATGGAAGAT 
GTTGTGGATT CTTCTCTGTG GATCGACCTC AGTAGTGATG CTCAGGAGTT TGAAGCAGAG 
TCTTGGAGTC TCGTGGTGGA CCCTTCCTTT TGCAGTAGGC AGGAGAAGGA TGTCATCAAA 
AGACAAGATG TCATTTTTGA GCTGATGCAG ACAGAGGTCC ACCACATCCA GACTCTGCTC 
ATCATGTCCG AGGTCTTCAG GAAAGGCATG AAGGAGGAAC TGCAGCTGGA CCACAGCACG 
GTGGACAAGA TCTTCCCCTG CTTAGATGAG CTGCTGGAGA CTCACCGGCA CTTCTTCTTC 
AGCATGAAGG AGCGCAGGCA GGAGTCCTGC GCTGGCAGTG ACAGGAACTT TGTGATCAAC 
CAAATTGGAG ACATTCTGGT ACAACAGTTT TCAGAGGAAA ATGCAAGCAA AATGAAGAGG 
ATCTATGGAG AATTCTGCAG CCATCACAAA GAAGCCATGA GTCTCTTTAA AGAGCTGCAG 
CAGAACAAGA AGTTTCAGAA TTTTATTAAG ATCCGAAATA GCAACCTGTT GGCTCGTCGC 
CGCGGAATCC CGGAGTGCAT CCTGCTAGTT ACCCAGCGCA TCACAAAGTA CCCAGTCTTG 
GTGGAAAGGA TCCTGCAGTA CACCAAGGAA AGGACTGAAG AGCACCGGGA CCTGTGCAAA 
GCTCTCGGCT TAATTAAAGA CATGATCGCA GCCGTGGACC TGAAAGTCAG CGAATACGAG 
AAGAACCAAA AATGGCTCGA GATCCTAAAT AAAATCGAGA ACAAAACATA CACAAAGCTC 
AAAAATGGCC ACGTGTTTAG AAAGCAGGCG CTGCTGAGCC AGGAACGGGC TCTCCTGCAT 
GATGGGCTTG TCTACTGGAA AACCGCTACG GGTCGCTTCA AAGACATCCT GGCTCTGCTA 
CTAACTGACG TGCTGCTCTT CTTACAAGAA AAAGACCAGA AATACATCTT TGCAGCTGTT 
GACCAGAAGC CATCAGTGAT TTCCCTCCAG AAGCTGATTG CTAGAGAAGT TGCCAACGAG 
GAGAGAGGCA TGTTTCTGAT CAGTGCTTCG TCAGCCGGCC CCGAGATGTA CGAAATCCAC 
ACCAATTCCA AGGAGGAGCG GAATAACTGG ATGAGGCGGA TCCAGCAGGC AGTAGAAAGT 
TGTCCAGAGG AAGAAGGAGG AAGGACGAGC GAGTCGGATG AAGAGAGGCG GAAAGCTGAA 
GCTAGAGTGG CCAAGATTCA GCAATGTCAA GAAATACTCA GTAACCAAGA CCAACAGATT 
TGCACGTACT TGGAAGAAAA GCTGCATATC TACGCTGAAC TTGGGGAACT GAGTGGCTTT 
GAGGATGTCC ACCTAGAACC CCATCTGCTC ATCAAACCTG ACCCTGGAGA GCCTCCCCAG 
GCAGCGTCCC TGCTTGCAGC AGCCCTGAGA GAAGCCGAGA GTCTGCAAGT GGCTGTGAAG 
GCTTCAAAGA TGGGCGATGT AAGCCAGTCA TCCGAAGAAA GCCCTGGAGG GACTGTCCTG 
ATGGACACAC CCAGTACACA GGATGTACCA GCGTCACCAA CTGCTTCATT AGTCACAGAA 
GGGACAGAAG GAAGAGGCTG TTGGGATGTG GATCCCGGGC TCCAGGGTGT GGTAACCGAC 
TTGGCAGTCT CTGATGCAGG GGAGAAGGTG GAATATAGAA GTTTCTCCGG TTCTTCACAG 
TCAGAGATTA TACAAGCAAT ACAGAATTTA ACGCGTCTCC TCTACAGCCT TCAGGCTGCC 
TTGACCATCC AGGACAGTCA CATTGAGATC CACAAACTGG TTCTACAGCA GCGGGAGAGC 
TTGGCCCCTA GCCACTCCTT CCGAGGTGGC CCCTTGCAAG ACCAGGAGAA ATCCCGCTAC 
CTGGAAAAGC AGCGTGAAGA GCTGGCCAAC ATCCACAAGC TTCAACACCA GTTCCAGCAG 
GAGCAGCGGC GCTGGCACCG CACGTGCGAC CAGCAACAGC GCGAGCAGGA AGCCCAAGAG 
AGCTGGCTGC AGGCGCGGGA ACGTGAGTGC CAGTCCCAGG AGGAATTGTT GCTGCGCCAC 
CGTAGCGAGC TGGACCACCA ACTCCAGGAG TACCAGCAGA GCCTTGAGCG GCTACGGGAG 
GGCCAGCGGA TGGTGGAGAG GGAGAGACAG AAGATGCGTG TCCAGCAGGG TCTGCTGGGC 
CACTGCAAGC ATAGTCGCCA GCGGAGCCTC CCAGCCGTCT TCTCTCCAGG GAGCAAGGAG 
GTAACAGAAC TTAACCGAGC TGAGAGTTTG TGTCATGAAA ATTCATTTTT CATCAATGAA 
GCTTTTGGAC ACATGTCACT TAACACGTCC AATAAACCAA ACCCATCAGG GGTCCCCTGG 
GATGCTCACC CCCTGGAGGG GTCTCATTTT GATTTGGCAA GGACTAGTGA GAGTCCAACA 
GAACTGAAGA TAGACATTTC TCAGCCTCCT GATGTCAACT CTGAACTCTG GACAACGGGG 
CCGGGCCATC AGAGACCTGC TCTCCAAGAA AACAGCAAGG AGTCTTATAA AAATGTTGCA 
GATTTGGACT CCTTCCAGAG TGAATCGTCA TCTCCCCAGG ACTCCAATCA GCGTGGCCCC 
CAGCCACAGA CACTCACAAC AGAAGCAAAA CTAAGTCTTC CGATGGCGGC TGGACACGGT 
GGCGATGCTG GAGACGGGGC TGAAGAAAAC ATTCTTTACC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgef28 ( XM_006517519.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517582.1 Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X3, mRNA.

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CloneID	OMu48947
Clone ID Related Accession (Same CDS sequence)	XM_006517519.4 , XM_006517519.3
Accession Version	XM_006517519.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4164bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	rho guanine nucleotide exchange factor 28 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006517519.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCTG ACTCGGACTC ACCTTTCAAC TACTCCTGGC CTTCCTTCCC CAAGATGAAG 
ATTCGGCGCA GGGCATCCAA GAAAGATCGC TCCTTTGATG GCCTCAAAAA ATCCAAGCAT 
GTCCCAGCGT CCCTTGCTGC AGGCCAGCTC TCTGATGTTC TGAACGGGGG TGATGAAGTC 
TACGCTAACT GTATGGTGAT TGACCAGGTT GGCGATTTGG ATATCAACTA TATAAACCTA 
GAGGGACTCT CCACCCATAC GAGTCCCGAG TCTGGGAGGT CCATGCTGGG GCCTCAGGCT 
TGCATGCACA CCCTTCCTCC CGACACCAGT CCCTGCGGGC GCCCACTCAT TGAGAACAGT 
GAGGGGACCC TGGATGCTGC AGCTTCGCAG TCTTTCGTGA CACCATCCAG TTCTCGCACT 
TCCAACTTGA ACCTTTCTTT TGGTCTCCAT GGATTTGAAA AGGAGCAGAG TCATCTCAAG 
AAAAGGAGTT CTAGCCTGGA TGCCCTGGTT GCCGACAGTG AGGGGGAAGG AGGTTCTGAG 
CCCCCCATCT GCTATGCTGT GGGCTCTCAG AGTTCCCCAA GAACTGGACT CCCCAGCGGG 
GATGAACTGG ACTCTTTTGA GACCAACACT GAACCAGACT GTAATATCTC CAGGACCGAA 
TCACTCTCTC TGTCCAGTAC TCTGCATTCA AAGGAGTCCT TGCTTTCTGG GATCCGGTCT 
CGCTCCTACT CCTGCTCATC ACCCAAAATC TCTTCAGGAA AATCCCGGCT AGTGCGCGAC 
TTCACAGTGT GCAGTACCAG CGAAGAACAG AGATCATATA GTTTCCAGGA GCCACCAGGA 
GAAAAAAGGA TTCAGGAAGA AGAATGGGAT GAATACGTCA TTCCTGCCAA ATCCGAGTCT 
GAAAAGTACA AAGTGAGCCG AACTTTCAGC TTCCTCATGA ACAGGATGAC CAGCCCACGG 
AACAAATCTA AGATGAAAAA CAAGGACACT AAAGAAAAAG AAAAAATGAA TCGACATCAG 
TTTGTCCCGG GAACGTTCTC CGGGGTTCTG CAGTGCTCCG GTTGTGATAA AACACTCCTG 
GGGAAAGAGT CACTCCAGTG TGCCAACTGT AAGGCAAACA CACACAAGGG CTGCAAAGAT 
GCTGTGCCTC CGTGTACCAA GAAATTCCAA GAGAAATACA ACAAGAACAA ACCACAGAGC 
ATCCTTGGAA GCTCTTCAGT CAGAGACGTC CCAGCACCTG GGCTCTCCTT GCACCCTTCT 
TCCTCTATGC CCATCGGACT GCCAGCTGGA AGGAAGGAAT TTGCGGCTCA GGTGCATCCA 
CTGTCCAGAA GTGTCCCCGG CACCACTTTG GAAAGCTTCA GGAGGGCAGT GACATCGTTA 
GAGTCTGAGG GTGACAGCTG GAGAAGCAGG TCTCATTCCG ATGAGCTGTT CCAGTCTATG 
GGCTCTTCTC CTTCCACGGA GTCATTCATG ATGGAAGATG TTGTGGATTC TTCTCTGTGG 
ATCGACCTCA GTAGTGATGC TCAGGAGTTT GAAGCAGAGT CTTGGAGTCT CGTGGTGGAC 
CCTTCCTTTT GCAGTAGGCA GGAGAAGGAT GTCATCAAAA GACAAGATGT CATTTTTGAG 
CTGATGCAGA CAGAGGTCCA CCACATCCAG ACTCTGCTCA TCATGTCCGA GGTCTTCAGG 
AAAGGCATGA AGGAGGAACT GCAGCTGGAC CACAGCACGG TGGACAAGAT CTTCCCCTGC 
TTAGATGAGC TGCTGGAGAC TCACCGGCAC TTCTTCTTCA GCATGAAGGA GCGCAGGCAG 
GAGTCCTGCG CTGGCAGTGA CAGGAACTTT GTGATCAACC AAATTGGAGA CATTCTGGTA 
CAACAGTTTT CAGAGGAAAA TGCAAGCAAA ATGAAGAGGA TCTATGGAGA ATTCTGCAGC 
CATCACAAAG AAGCCATGAG TCTCTTTAAA GAGCTGCAGC AGAACAAGAA GTTTCAGAAT 
TTTATTAAGA TCCGAAATAG CAACCTGTTG GCTCGTCGCC GCGGAATCCC GGAGTGCATC 
CTGCTAGTTA CCCAGCGCAT CACAAAGTAC CCAGTCTTGG TGGAAAGGAT CCTGCAGTAC 
ACCAAGGAAA GGACTGAAGA GCACCGGGAC CTGTGCAAAG CTCTCGGCTT AATTAAAGAC 
ATGATCGCAG CCGTGGACCT GAAAGTCAGC GAATACGAGA AGAACCAAAA ATGGCTCGAG 
ATCCTAAATA AAATCGAGAA CAAAACATAC ACAAAGCTCA AAAATGGCCA CGTGTTTAGA 
AAGCAGGCGC TGCTGAGCCA GGAACGGGCT CTCCTGCATG ATGGGCTTGT CTACTGGAAA 
ACCGCTACGG GTCGCTTCAA AGACATCCTG GCTCTGCTAC TAACTGACGT GCTGCTCTTC 
TTACAAGAAA AAGACCAGAA ATACATCTTT GCAGCTGTTG ACCAGAAGCC ATCAGTGATT 
TCCCTCCAGA AGCTGATTGC TAGAGAAGTT GCCAACGAGG AGAGAGGCAT GTTTCTGATC 
AGTGCTTCGT CAGCCGGCCC CGAGATGTAC GAAATCCACA CCAATTCCAA GGAGGAGCGG 
AATAACTGGA TGAGGCGGAT CCAGCAGGCA GTAGAAAGTT GTCCAGAGGA AGAAGGAGGA 
AGGACGAGCG AGTCGGATGA AGAGAGGCGG AAAGCTGAAG CTAGAGTGGC CAAGATTCAG 
CAATGTCAAG AAATACTCAG TAACCAAGAC CAACAGATTT GCACGTACTT GGAAGAAAAG 
CTGCATATCT ACGCTGAACT TGGGGAACTG AGTGGCTTTG AGGATGTCCA CCTAGAACCC 
CATCTGCTCA TCAAACCTGA CCCTGGAGAG CCTCCCCAGG CAGCGTCCCT GCTTGCAGCA 
GCCCTGAGAG AAGCCGAGAG TCTGCAAGTG GCTGTGAAGG CTTCAAAGAT GGGCGATGTA 
AGCCAGTCAT CCGAAGAAAG CCCTGGAGGG ACTGTCCTGA TGGACACACC CAGTACACAG 
GATGTACCAG CGTCACCAAC TGCTTCATTA GTCACAGAAG GGACAGAAGG AAGAGGCTGT 
TGGGATGTGG ATCCCGGGCT CCAGGGTGTG GTAACCGACT TGGCAGTCTC TGATGCAGGG 
GAGAAGGTGG AATATAGAAG TTTCTCCGGT TCTTCACAGT CAGAGATTAT ACAAGCAATA 
CAGAATTTAA CGCGTCTCCT CTACAGCCTT CAGGCTGCCT TGACCATCCA GGACAGTCAC 
ATTGAGATCC ACAAACTGGT TCTACAGCAG CGGGAGAGCT TGGCCCCTAG CCACTCCTTC 
CGAGGTGGCC CCTTGCAAGA CCAGGAGAAA TCCCGCTACC TGGAAAAGCA GCGTGAAGAG 
CTGGCCAACA TCCACAAGCT TCAACACCAG TTCCAGCAGG AGCAGCGGCG CTGGCACCGC 
ACGTGCGACC AGCAACAGCG CGAGCAGGAA GCCCAAGAGA GCTGGCTGCA GGCGCGGGAA 
CGTGAGTGCC AGTCCCAGGA GGAATTGTTG CTGCGCCACC GTAGCGAGCT GGACCACCAA 
CTCCAGGAGT ACCAGCAGAG CCTTGAGCGG CTACGGGAGG GCCAGCGGAT GGTGGAGAGG 
GAGAGACAGA AGATGCGTGT CCAGCAGGGT CTGCTGGGCC ACTGCAAGCA TAGTCGCCAG 
CGGAGCCTCC CAGCCGTCTT CTCTCCAGGG AGCAAGGAGG TAACAGAACT TAACCGAGCT 
GAGAGTTTGT GTCATGAAAA TTCATTTTTC ATCAATGAAG CTTTTGGACA CATGTCACTT 
AACACGTCCA ATAAACCAAA CCCATCAGGG GTCCCCTGGG ATGCTCACCC CCTGGAGGGG 
TCTCATTTTG ATTTGGCAAG GACTAGTGAG AGTCCAACAG AACTGAAGAT AGACATTTCT 
CAGCCTCCTG ATGTCAACTC TGAACTCTGG ACAACGGGGC CGGGCCATCA GAGACCTGCT 
CTCCAAGAAA ACAGCAAGGA GTCTTATAAA AATGTTGCAG ATTTGGACTC CTTCCAGAGT 
GAATCGTCAT CTCCCCAGGA CTCCAATCAG CGTGGCCCCC AGCCACAGAC ACTCACAACA 
GAAGCAAAAC TAAGTCTTCC GATGGCGGCT GGACACGGTG GCGATGCTGG AGACGGGGCT 
GAAGAAAACA TTCTTTACCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517582.1
CDS	1485..5648
Translation	MDSDSDSPFNYSWPSFPKMKIRRRASKKDRSFDGLKKSKHVPASLAAGQLSDVLNGGDEVYANCMVIDQVGDLDINYINLEGLSTHTSPESGRSMLGPQACMHTLPPDTSPCGRPLIENSEGTLDAAASQSFVTPSSSRTSNLNLSFGLHGFEKEQSHLKKRSSSLDALVADSEGEGGSEPPICYAVGSQSSPRTGLPSGDELDSFETNTEPDCNISRTESLSLSSTLHSKESLLSGIRSRSYSCSSPKISSGKSRLVRDFTVCSTSEEQRSYSFQEPPGEKRIQEEEWDEYVIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKMKNKDTKEKEKMNRHQFVPGTFSGVLQCSGCDKTLLGKESLQCANCKANTHKGCKDAVPPCTKKFQEKYNKNKPQSILGSSSVRDVPAPGLSLHPSSSMPIGLPAGRKEFAAQVHPLSRSVPGTTLESFRRAVTSLESEGDSWRSRSHSDELFQSMGSSPSTESFMMEDVVDSSLWIDLSSDAQEFEAESWSLVVDPSFCSRQEKDVIKRQDVIFELMQTEVHHIQTLLIMSEVFRKGMKEELQLDHSTVDKIFPCLDELLETHRHFFFSMKERRQESCAGSDRNFVINQIGDILVQQFSEENASKMKRIYGEFCSHHKEAMSLFKELQQNKKFQNFIKIRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKERTEEHRDLCKALGLIKDMIAAVDLKVSEYEKNQKWLEILNKIENKTYTKLKNGHVFRKQALLSQERALLHDGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNNWMRRIQQAVESCPEEEGGRTSESDEERRKAEARVAKIQQCQEILSNQDQQICTYLEEKLHIYAELGELSGFEDVHLEPHLLIKPDPGEPPQAASLLAAALREAESLQVAVKASKMGDVSQSSEESPGGTVLMDTPSTQDVPASPTASLVTEGTEGRGCWDVDPGLQGVVTDLAVSDAGEKVEYRSFSGSSQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIHKLVLQQRESLAPSHSFRGGPLQDQEKSRYLEKQREELANIHKLQHQFQQEQRRWHRTCDQQQREQEAQESWLQARERECQSQEELLLRHRSELDHQLQEYQQSLERLREGQRMVERERQKMRVQQGLLGHCKHSRQRSLPAVFSPGSKEVTELNRAESLCHENSFFINEAFGHMSLNTSNKPNPSGVPWDAHPLEGSHFDLARTSESPTELKIDISQPPDVNSELWTTGPGHQRPALQENSKESYKNVADLDSFQSESSSPQDSNQRGPQPQTLTTEAKLSLPMAAGHGGDAGDGAEENILYL

Target ORF information:

RefSeq Version	XM_006517519.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006517519.3

ORF Insert Sequence:

ATGGATTCTG ACTCGGACTC ACCTTTCAAC TACTCCTGGC CTTCCTTCCC CAAGATGAAG 
ATTCGGCGCA GGGCATCCAA GAAAGATCGC TCCTTTGATG GCCTCAAAAA ATCCAAGCAT 
GTCCCAGCGT CCCTTGCTGC AGGCCAGCTC TCTGATGTTC TGAACGGGGG TGATGAAGTC 
TACGCTAACT GTATGGTGAT TGACCAGGTT GGCGATTTGG ATATCAACTA TATAAACCTA 
GAGGGACTCT CCACCCATAC GAGTCCCGAG TCTGGGAGGT CCATGCTGGG GCCTCAGGCT 
TGCATGCACA CCCTTCCTCC CGACACCAGT CCCTGCGGGC GCCCACTCAT TGAGAACAGT 
GAGGGGACCC TGGATGCTGC AGCTTCGCAG TCTTTCGTGA CACCATCCAG TTCTCGCACT 
TCCAACTTGA ACCTTTCTTT TGGTCTCCAT GGATTTGAAA AGGAGCAGAG TCATCTCAAG 
AAAAGGAGTT CTAGCCTGGA TGCCCTGGTT GCCGACAGTG AGGGGGAAGG AGGTTCTGAG 
CCCCCCATCT GCTATGCTGT GGGCTCTCAG AGTTCCCCAA GAACTGGACT CCCCAGCGGG 
GATGAACTGG ACTCTTTTGA GACCAACACT GAACCAGACT GTAATATCTC CAGGACCGAA 
TCACTCTCTC TGTCCAGTAC TCTGCATTCA AAGGAGTCCT TGCTTTCTGG GATCCGGTCT 
CGCTCCTACT CCTGCTCATC ACCCAAAATC TCTTCAGGAA AATCCCGGCT AGTGCGCGAC 
TTCACAGTGT GCAGTACCAG CGAAGAACAG AGATCATATA GTTTCCAGGA GCCACCAGGA 
GAAAAAAGGA TTCAGGAAGA AGAATGGGAT GAATACGTCA TTCCTGCCAA ATCCGAGTCT 
GAAAAGTACA AAGTGAGCCG AACTTTCAGC TTCCTCATGA ACAGGATGAC CAGCCCACGG 
AACAAATCTA AGATGAAAAA CAAGGACACT AAAGAAAAAG AAAAAATGAA TCGACATCAG 
TTTGTCCCGG GAACGTTCTC CGGGGTTCTG CAGTGCTCCG GTTGTGATAA AACACTCCTG 
GGGAAAGAGT CACTCCAGTG TGCCAACTGT AAGGCAAACA CACACAAGGG CTGCAAAGAT 
GCTGTGCCTC CGTGTACCAA GAAATTCCAA GAGAAATACA ACAAGAACAA ACCACAGAGC 
ATCCTTGGAA GCTCTTCAGT CAGAGACGTC CCAGCACCTG GGCTCTCCTT GCACCCTTCT 
TCCTCTATGC CCATCGGACT GCCAGCTGGA AGGAAGGAAT TTGCGGCTCA GGTGCATCCA 
CTGTCCAGAA GTGTCCCCGG CACCACTTTG GAAAGCTTCA GGAGGGCAGT GACATCGTTA 
GAGTCTGAGG GTGACAGCTG GAGAAGCAGG TCTCATTCCG ATGAGCTGTT CCAGTCTATG 
GGCTCTTCTC CTTCCACGGA GTCATTCATG ATGGAAGATG TTGTGGATTC TTCTCTGTGG 
ATCGACCTCA GTAGTGATGC TCAGGAGTTT GAAGCAGAGT CTTGGAGTCT CGTGGTGGAC 
CCTTCCTTTT GCAGTAGGCA GGAGAAGGAT GTCATCAAAA GACAAGATGT CATTTTTGAG 
CTGATGCAGA CAGAGGTCCA CCACATCCAG ACTCTGCTCA TCATGTCCGA GGTCTTCAGG 
AAAGGCATGA AGGAGGAACT GCAGCTGGAC CACAGCACGG TGGACAAGAT CTTCCCCTGC 
TTAGATGAGC TGCTGGAGAC TCACCGGCAC TTCTTCTTCA GCATGAAGGA GCGCAGGCAG 
GAGTCCTGCG CTGGCAGTGA CAGGAACTTT GTGATCAACC AAATTGGAGA CATTCTGGTA 
CAACAGTTTT CAGAGGAAAA TGCAAGCAAA ATGAAGAGGA TCTATGGAGA ATTCTGCAGC 
CATCACAAAG AAGCCATGAG TCTCTTTAAA GAGCTGCAGC AGAACAAGAA GTTTCAGAAT 
TTTATTAAGA TCCGAAATAG CAACCTGTTG GCTCGTCGCC GCGGAATCCC GGAGTGCATC 
CTGCTAGTTA CCCAGCGCAT CACAAAGTAC CCAGTCTTGG TGGAAAGGAT CCTGCAGTAC 
ACCAAGGAAA GGACTGAAGA GCACCGGGAC CTGTGCAAAG CTCTCGGCTT AATTAAAGAC 
ATGATCGCAG CCGTGGACCT GAAAGTCAGC GAATACGAGA AGAACCAAAA ATGGCTCGAG 
ATCCTAAATA AAATCGAGAA CAAAACATAC ACAAAGCTCA AAAATGGCCA CGTGTTTAGA 
AAGCAGGCGC TGCTGAGCCA GGAACGGGCT CTCCTGCATG ATGGGCTTGT CTACTGGAAA 
ACCGCTACGG GTCGCTTCAA AGACATCCTG GCTCTGCTAC TAACTGACGT GCTGCTCTTC 
TTACAAGAAA AAGACCAGAA ATACATCTTT GCAGCTGTTG ACCAGAAGCC ATCAGTGATT 
TCCCTCCAGA AGCTGATTGC TAGAGAAGTT GCCAACGAGG AGAGAGGCAT GTTTCTGATC 
AGTGCTTCGT CAGCCGGCCC CGAGATGTAC GAAATCCACA CCAATTCCAA GGAGGAGCGG 
AATAACTGGA TGAGGCGGAT CCAGCAGGCA GTAGAAAGTT GTCCAGAGGA AGAAGGAGGA 
AGGACGAGCG AGTCGGATGA AGAGAGGCGG AAAGCTGAAG CTAGAGTGGC CAAGATTCAG 
CAATGTCAAG AAATACTCAG TAACCAAGAC CAACAGATTT GCACGTACTT GGAAGAAAAG 
CTGCATATCT ACGCTGAACT TGGGGAACTG AGTGGCTTTG AGGATGTCCA CCTAGAACCC 
CATCTGCTCA TCAAACCTGA CCCTGGAGAG CCTCCCCAGG CAGCGTCCCT GCTTGCAGCA 
GCCCTGAGAG AAGCCGAGAG TCTGCAAGTG GCTGTGAAGG CTTCAAAGAT GGGCGATGTA 
AGCCAGTCAT CCGAAGAAAG CCCTGGAGGG ACTGTCCTGA TGGACACACC CAGTACACAG 
GATGTACCAG CGTCACCAAC TGCTTCATTA GTCACAGAAG GGACAGAAGG AAGAGGCTGT 
TGGGATGTGG ATCCCGGGCT CCAGGGTGTG GTAACCGACT TGGCAGTCTC TGATGCAGGG 
GAGAAGGTGG AATATAGAAG TTTCTCCGGT TCTTCACAGT CAGAGATTAT ACAAGCAATA 
CAGAATTTAA CGCGTCTCCT CTACAGCCTT CAGGCTGCCT TGACCATCCA GGACAGTCAC 
ATTGAGATCC ACAAACTGGT TCTACAGCAG CGGGAGAGCT TGGCCCCTAG CCACTCCTTC 
CGAGGTGGCC CCTTGCAAGA CCAGGAGAAA TCCCGCTACC TGGAAAAGCA GCGTGAAGAG 
CTGGCCAACA TCCACAAGCT TCAACACCAG TTCCAGCAGG AGCAGCGGCG CTGGCACCGC 
ACGTGCGACC AGCAACAGCG CGAGCAGGAA GCCCAAGAGA GCTGGCTGCA GGCGCGGGAA 
CGTGAGTGCC AGTCCCAGGA GGAATTGTTG CTGCGCCACC GTAGCGAGCT GGACCACCAA 
CTCCAGGAGT ACCAGCAGAG CCTTGAGCGG CTACGGGAGG GCCAGCGGAT GGTGGAGAGG 
GAGAGACAGA AGATGCGTGT CCAGCAGGGT CTGCTGGGCC ACTGCAAGCA TAGTCGCCAG 
CGGAGCCTCC CAGCCGTCTT CTCTCCAGGG AGCAAGGAGG TAACAGAACT TAACCGAGCT 
GAGAGTTTGT GTCATGAAAA TTCATTTTTC ATCAATGAAG CTTTTGGACA CATGTCACTT 
AACACGTCCA ATAAACCAAA CCCATCAGGG GTCCCCTGGG ATGCTCACCC CCTGGAGGGG 
TCTCATTTTG ATTTGGCAAG GACTAGTGAG AGTCCAACAG AACTGAAGAT AGACATTTCT 
CAGCCTCCTG ATGTCAACTC TGAACTCTGG ACAACGGGGC CGGGCCATCA GAGACCTGCT 
CTCCAAGAAA ACAGCAAGGA GTCTTATAAA AATGTTGCAG ATTTGGACTC CTTCCAGAGT 
GAATCGTCAT CTCCCCAGGA CTCCAATCAG CGTGGCCCCC AGCCACAGAC ACTCACAACA 
GAAGCAAAAC TAAGTCTTCC GATGGCGGCT GGACACGGTG GCGATGCTGG AGACGGGGCT 
GAAGAAAACA TTCTTTACCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgef28 ( XM_006517521.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517584.1 Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X6, mRNA.

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CloneID	OMu48949
Clone ID Related Accession (Same CDS sequence)	XM_006517521.3 , XM_006517521.4
Accession Version	XM_006517521.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	rho guanine nucleotide exchange factor 28 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006517521.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCGGC GACGAGCCCG GCTGGCCAGA GATGCAGGAG CCATGGAGTT GAGCTGCAGT 
GAAGTGCCTC TTTATGGACA GAAGACGGTC TATGCAAAGT TTGGCAAAAA CGTGTATCTA 
CCCGAAGATG CGGAGTTTTA CTTTGTTTAT GGCGGGTCGC ATCAGAGGCA CGTGGTGATT 
GCTGACCGTG TCCAGGACAA TGTCCTCCAA TCTAGCATTC CAGGCCACTG GCTTCAGGAG 
ACGGTGACGG TATCTGTGTG CCTCTGCTCG GAAGGGTACT CCCCAGTGAC CATGGGCTCT 
GGCTCAGTGA CCTACGTGGA CAACATGGCC TGCAGGCTGG CTCGTCTGCT GGTCACTCAG 
GCTGACCGAC TCACGGCCTG CAGTCACCAG ACGCTGCTGA CCCCGTTTGC CCTGACAGTG 
GAAGCGCTGC CTGCCTTGGA TGAGGAGCTC GTGCTGGCTC TGACCCAACT GGAGCTGCCT 
TTGGGTTGGA CAGTCCTGGG AAATTCCTCA CTTGAAGTAT CTCTACACAG AGAGTCTCTC 
CTGCATCTGG CTGTGAGATG GGCCCTCCCT AAGCTATTCC ACTTCCTCTT GTGTCTCCCT 
GGAGGAGTCA AGGCCTTGAA GCTACCTAAT GAAGAGGCCA CCACACCATT AGACTTAGCT 
TTGCAAGGTG GACACTCCAC ACTGGTAGAA GACATCACAA ATTTTCAGGG TAGCCATTCC 
CCAGGCTTCT CCCGACTGCG TCTTAACGAA GAGGCCACTC TGCAGTTTGT TCATTCATCG 
GAAACATTGA CCCTGACCGT TAACCACACA GCTGAGCATC TCTTGGAAGC AGACATCAAA 
CTCTTCCGGA AATATTTCTG GGACAGAGCC TTTCTTGTCA AGGCTCTTGA GCAAGAAGCC 
AAGACTGAGA AAGCAACCAT GCCTTCTGGA GCTGCAGAAA CTGAAGAAGA GGTTAGGAAT 
TTGGAGTCTG GCAGATCACC ATCGGAGGAG GAGGAGGATG CAAAGAGCAT CAAAAGCCAG 
GTGGATGGGC CGAGTGAACA CGAAGACCAG GACCGCCTAC CTCTGGATCG CTCCTTTGAT 
GGCCTCAAAA AATCCAAGCA TGTCCCAGCG TCCCTTGCTG CAGGCCAGCT CTCTGATGTT 
CTGAACGGGG GTGATGAAGT CTACGCTAAC TGTATGGTGA TTGACCAGGT TGGCGATTTG 
GATATCAACT ATATAAACCT AGAGGGACTC TCCACCCATA CGAGTCCCGA GTCTGGGAGG 
TCCATGCTGG GGCCTCAGGC TTGCATGCAC ACCCTTCCTC CCGACACCAG TCCCTGCGGG 
CGCCCACTCA TTGAGAACAG TGAGGGGACC CTGGATGCTG CAGCTTCGCA GTCTTTCGTG 
ACACCATCCA GTTCTCGCAC TTCCAACTTG AACCTTTCTT TTGGTCTCCA TGGATTTGAA 
AAGGAGCAGA GTCATCTCAA GAAAAGGAGT TCTAGCCTGG ATGCCCTGGT TGCCGACAGT 
GAGGGGGAAG GAGGTTCTGA GCCCCCCATC TGCTATGCTG TGGGCTCTCA GAGTTCCCCA 
AGAACTGGAC TCCCCAGCGG GGATGAACTG GACTCTTTTG AGACCAACAC TGAACCAGAC 
TGTAATATCT CCAGGACCGA ATCACTCTCT CTGTCCAGTA CTCTGCATTC AAAGGAGTCC 
TTGCTTTCTG GGATCCGGTC TCGCTCCTAC TCCTGCTCAT CACCCAAAAT CTCTTCAGGA 
AAATCCCGGC TAGTGCGCGA CTTCACAGTG TGCAGTACCA GCGAAGAACA GAGATCATAT 
AGTTTCCAGG AGCCACCAGG AGAAAAAAGG ATTCAGGAAG AAGAATGGGA TGAATACGTC 
ATTCCTGCCA AATCCGAGTC TGAAAAGTAC AAAGTGAGCC GAACTTTCAG CTTCCTCATG 
AACAGGATGA CCAGCCCACG GAACAAATCT AAGATGAAAA ACAAGGACAC TAAAGAAAAA 
GAAAAAATGA ATCGACATCA GTTTGTCCCG GGAACGTTCT CCGGGGTTCT GCAGTGCTCC 
GGTTGTGATA AAACACTCCT GGGGAAAGAG TCACTCCAGT GTGCCAACTG TAAGGCAAAC 
ACACACAAGG GCTGCAAAGA TGCTGTGCCT CCGTGTACCA AGAAATTCCA AGAGAAATAC 
AACAAGAACA AACCACAGAG CATCCTTGGA AGCTCTTCAG TCAGAGACGT CCCAGCACCT 
GGGCTCTCCT TGCACCCTTC TTCCTCTATG CCCATCGGAC TGCCAGCTGG AAGGAAGGAA 
TTTGCGGCTC AGGTGCATCC ACTGTCCAGA AGTGTCCCCG GCACCACTTT GGAAAGCTTC 
AGGAGGGCAG TGACATCGTT AGAGTCTGAG GGTGACAGCT GGAGAAGCAG GTCTCATTCC 
GATGAGCTGT TCCAGTCTAT GGGCTCTTCT CCTTCCACGG AGTCATTCAT GATGGAAGAT 
GTTGTGGATT CTTCTCTGTG GATCGACCTC AGTAGTGATG CTCAGGAGTT TGAAGCAGAG 
TCTTGGAGTC TCGTGGTGGA CCCTTCCTTT TGCAGTAGGC AGGAGAAGGA TGTCATCAAA 
AGACAAGATG TCATTTTTGA GCTGATGCAG ACAGAGGTCC ACCACATCCA GACTCTGCTC 
ATCATGTCCG AGGTCTTCAG GAAAGGCATG AAGGAGGAAC TGCAGCTGGA CCACAGCACG 
GTGGACAAGA TCTTCCCCTG CTTAGATGAG CTGCTGGAGA CTCACCGGCA CTTCTTCTTC 
AGCATGAAGG AGCGCAGGCA GGAGTCCTGC GCTGGCAGTG ACAGGAACTT TGTGATCAAC 
CAAATTGGAG ACATTCTGGT ACAACAGTTT TCAGAGGAAA ATGCAAGCAA AATGAAGAGG 
ATCTATGGAG AATTCTGCAG CCATCACAAA GAAGCCATGA GTCTCTTTAA AGAGCTGCAG 
CAGAACAAGA AGTTTCAGAA TTTTATTAAG ATCCGAAATA GCAACCTGTT GGCTCGTCGC 
CGCGGAATCC CGGAGTGCAT CCTGCTAGTT ACCCAGCGCA TCACAAAGTA CCCAGTCTTG 
GTGGAAAGGA TCCTGCAGTA CACCAAGGAA AGGACTGAAG AGCACCGGGA CCTGTGCAAA 
GCTCTCGGCT TAATTAAAGA CATGATCGCA GCCGTGGACC TGAAAGTCAG CGAATACGAG 
AAGAACCAAA AATGGCTCGA GATCCTAAAT AAAATCGAGA ACAAAACATA CACAAAGCTC 
AAAAATGGCC ACGTGTTTAG AAAGCAGGCG CTGCTGAGCC AGGAACGGGC TCTCCTGCAT 
GATGGGCTTG TCTACTGGAA AACCGCTACG GGTCGCTTCA AAGACATCCT GGCTCTGCTA 
CTAACTGACG TGCTGCTCTT CTTACAAGAA AAAGACCAGA AATACATCTT TGCAGCTGTT 
GACCAGAAGC CATCAGTGAT TTCCCTCCAG AAGCTGATTG CTAGAGAAGT TGCCAACGAG 
GAGAGAGGCA TGTTTCTGAT CAGTGCTTCG TCAGCCGGCC CCGAGATGTA CGAAATCCAC 
ACCAATTCCA AGGAGGAGCG GAATAACTGG ATGAGGCGGA TCCAGCAGGC AGTAGAAAGT 
TGTCCAGAGG AAGAAGGAGG AAGGACGAGC GAGTCGGATG AAGAGAGGCG GAAAGCTGAA 
GCTAGAGTGG CCAAGATTCA GCAATGTCAA GAAATACTCA GTAACCAAGA CCAACAGATT 
TGCACGTACT TGGAAGAAAA GCTGCATATC TACGCTGAAC TTGGGGAACT GAGTGGCTTT 
GAGGATGTCC ACCTAGAACC CCATCTGCTC ATCAAACCTG ACCCTGGAGA GCCTCCCCAG 
GCAGCGTCCC TGCTTGCAGC AGCCCTGAGA GAAGCCGAGA GTCTGCAAGT GGCTGTGAAG 
GCTTCAAAGA TGGGCGATGT AAGCCAGTCA TCCGAAGAAA GCCCTGGAGG GACTGTCCTG 
ATGGACACAC CCAGTACACA GGATGTACCA GCGTCACCAA CTGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517584.1
CDS	271..4278
Translation	MRRRRARLARDAGAMELSCSEVPLYGQKTVYAKFGKNVYLPEDAEFYFVYGGSHQRHVVIADRVQDNVLQSSIPGHWLQETVTVSVCLCSEGYSPVTMGSGSVTYVDNMACRLARLLVTQADRLTACSHQTLLTPFALTVEALPALDEELVLALTQLELPLGWTVLGNSSLEVSLHRESLLHLAVRWALPKLFHFLLCLPGGVKALKLPNEEATTPLDLALQGGHSTLVEDITNFQGSHSPGFSRLRLNEEATLQFVHSSETLTLTVNHTAEHLLEADIKLFRKYFWDRAFLVKALEQEAKTEKATMPSGAAETEEEVRNLESGRSPSEEEEDAKSIKSQVDGPSEHEDQDRLPLDRSFDGLKKSKHVPASLAAGQLSDVLNGGDEVYANCMVIDQVGDLDINYINLEGLSTHTSPESGRSMLGPQACMHTLPPDTSPCGRPLIENSEGTLDAAASQSFVTPSSSRTSNLNLSFGLHGFEKEQSHLKKRSSSLDALVADSEGEGGSEPPICYAVGSQSSPRTGLPSGDELDSFETNTEPDCNISRTESLSLSSTLHSKESLLSGIRSRSYSCSSPKISSGKSRLVRDFTVCSTSEEQRSYSFQEPPGEKRIQEEEWDEYVIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKMKNKDTKEKEKMNRHQFVPGTFSGVLQCSGCDKTLLGKESLQCANCKANTHKGCKDAVPPCTKKFQEKYNKNKPQSILGSSSVRDVPAPGLSLHPSSSMPIGLPAGRKEFAAQVHPLSRSVPGTTLESFRRAVTSLESEGDSWRSRSHSDELFQSMGSSPSTESFMMEDVVDSSLWIDLSSDAQEFEAESWSLVVDPSFCSRQEKDVIKRQDVIFELMQTEVHHIQTLLIMSEVFRKGMKEELQLDHSTVDKIFPCLDELLETHRHFFFSMKERRQESCAGSDRNFVINQIGDILVQQFSEENASKMKRIYGEFCSHHKEAMSLFKELQQNKKFQNFIKIRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKERTEEHRDLCKALGLIKDMIAAVDLKVSEYEKNQKWLEILNKIENKTYTKLKNGHVFRKQALLSQERALLHDGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNNWMRRIQQAVESCPEEEGGRTSESDEERRKAEARVAKIQQCQEILSNQDQQICTYLEEKLHIYAELGELSGFEDVHLEPHLLIKPDPGEPPQAASLLAAALREAESLQVAVKASKMGDVSQSSEESPGGTVLMDTPSTQDVPASPTA

Target ORF information:

RefSeq Version	XM_006517521.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho guanine nucleotide exchange factor (GEF) 28 (Arhgef28), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006517521.3

ORF Insert Sequence:

ATGAGGCGGC GACGAGCCCG GCTGGCCAGA GATGCAGGAG CCATGGAGTT GAGCTGCAGT 
GAAGTGCCTC TTTATGGACA GAAGACGGTC TATGCAAAGT TTGGCAAAAA CGTGTATCTA 
CCCGAAGATG CGGAGTTTTA CTTTGTTTAT GGCGGGTCGC ATCAGAGGCA CGTGGTGATT 
GCTGACCGTG TCCAGGACAA TGTCCTCCAA TCTAGCATTC CAGGCCACTG GCTTCAGGAG 
ACGGTGACGG TATCTGTGTG CCTCTGCTCG GAAGGGTACT CCCCAGTGAC CATGGGCTCT 
GGCTCAGTGA CCTACGTGGA CAACATGGCC TGCAGGCTGG CTCGTCTGCT GGTCACTCAG 
GCTGACCGAC TCACGGCCTG CAGTCACCAG ACGCTGCTGA CCCCGTTTGC CCTGACAGTG 
GAAGCGCTGC CTGCCTTGGA TGAGGAGCTC GTGCTGGCTC TGACCCAACT GGAGCTGCCT 
TTGGGTTGGA CAGTCCTGGG AAATTCCTCA CTTGAAGTAT CTCTACACAG AGAGTCTCTC 
CTGCATCTGG CTGTGAGATG GGCCCTCCCT AAGCTATTCC ACTTCCTCTT GTGTCTCCCT 
GGAGGAGTCA AGGCCTTGAA GCTACCTAAT GAAGAGGCCA CCACACCATT AGACTTAGCT 
TTGCAAGGTG GACACTCCAC ACTGGTAGAA GACATCACAA ATTTTCAGGG TAGCCATTCC 
CCAGGCTTCT CCCGACTGCG TCTTAACGAA GAGGCCACTC TGCAGTTTGT TCATTCATCG 
GAAACATTGA CCCTGACCGT TAACCACACA GCTGAGCATC TCTTGGAAGC AGACATCAAA 
CTCTTCCGGA AATATTTCTG GGACAGAGCC TTTCTTGTCA AGGCTCTTGA GCAAGAAGCC 
AAGACTGAGA AAGCAACCAT GCCTTCTGGA GCTGCAGAAA CTGAAGAAGA GGTTAGGAAT 
TTGGAGTCTG GCAGATCACC ATCGGAGGAG GAGGAGGATG CAAAGAGCAT CAAAAGCCAG 
GTGGATGGGC CGAGTGAACA CGAAGACCAG GACCGCCTAC CTCTGGATCG CTCCTTTGAT 
GGCCTCAAAA AATCCAAGCA TGTCCCAGCG TCCCTTGCTG CAGGCCAGCT CTCTGATGTT 
CTGAACGGGG GTGATGAAGT CTACGCTAAC TGTATGGTGA TTGACCAGGT TGGCGATTTG 
GATATCAACT ATATAAACCT AGAGGGACTC TCCACCCATA CGAGTCCCGA GTCTGGGAGG 
TCCATGCTGG GGCCTCAGGC TTGCATGCAC ACCCTTCCTC CCGACACCAG TCCCTGCGGG 
CGCCCACTCA TTGAGAACAG TGAGGGGACC CTGGATGCTG CAGCTTCGCA GTCTTTCGTG 
ACACCATCCA GTTCTCGCAC TTCCAACTTG AACCTTTCTT TTGGTCTCCA TGGATTTGAA 
AAGGAGCAGA GTCATCTCAA GAAAAGGAGT TCTAGCCTGG ATGCCCTGGT TGCCGACAGT 
GAGGGGGAAG GAGGTTCTGA GCCCCCCATC TGCTATGCTG TGGGCTCTCA GAGTTCCCCA 
AGAACTGGAC TCCCCAGCGG GGATGAACTG GACTCTTTTG AGACCAACAC TGAACCAGAC 
TGTAATATCT CCAGGACCGA ATCACTCTCT CTGTCCAGTA CTCTGCATTC AAAGGAGTCC 
TTGCTTTCTG GGATCCGGTC TCGCTCCTAC TCCTGCTCAT CACCCAAAAT CTCTTCAGGA 
AAATCCCGGC TAGTGCGCGA CTTCACAGTG TGCAGTACCA GCGAAGAACA GAGATCATAT 
AGTTTCCAGG AGCCACCAGG AGAAAAAAGG ATTCAGGAAG AAGAATGGGA TGAATACGTC 
ATTCCTGCCA AATCCGAGTC TGAAAAGTAC AAAGTGAGCC GAACTTTCAG CTTCCTCATG 
AACAGGATGA CCAGCCCACG GAACAAATCT AAGATGAAAA ACAAGGACAC TAAAGAAAAA 
GAAAAAATGA ATCGACATCA GTTTGTCCCG GGAACGTTCT CCGGGGTTCT GCAGTGCTCC 
GGTTGTGATA AAACACTCCT GGGGAAAGAG TCACTCCAGT GTGCCAACTG TAAGGCAAAC 
ACACACAAGG GCTGCAAAGA TGCTGTGCCT CCGTGTACCA AGAAATTCCA AGAGAAATAC 
AACAAGAACA AACCACAGAG CATCCTTGGA AGCTCTTCAG TCAGAGACGT CCCAGCACCT 
GGGCTCTCCT TGCACCCTTC TTCCTCTATG CCCATCGGAC TGCCAGCTGG AAGGAAGGAA 
TTTGCGGCTC AGGTGCATCC ACTGTCCAGA AGTGTCCCCG GCACCACTTT GGAAAGCTTC 
AGGAGGGCAG TGACATCGTT AGAGTCTGAG GGTGACAGCT GGAGAAGCAG GTCTCATTCC 
GATGAGCTGT TCCAGTCTAT GGGCTCTTCT CCTTCCACGG AGTCATTCAT GATGGAAGAT 
GTTGTGGATT CTTCTCTGTG GATCGACCTC AGTAGTGATG CTCAGGAGTT TGAAGCAGAG 
TCTTGGAGTC TCGTGGTGGA CCCTTCCTTT TGCAGTAGGC AGGAGAAGGA TGTCATCAAA 
AGACAAGATG TCATTTTTGA GCTGATGCAG ACAGAGGTCC ACCACATCCA GACTCTGCTC 
ATCATGTCCG AGGTCTTCAG GAAAGGCATG AAGGAGGAAC TGCAGCTGGA CCACAGCACG 
GTGGACAAGA TCTTCCCCTG CTTAGATGAG CTGCTGGAGA CTCACCGGCA CTTCTTCTTC 
AGCATGAAGG AGCGCAGGCA GGAGTCCTGC GCTGGCAGTG ACAGGAACTT TGTGATCAAC 
CAAATTGGAG ACATTCTGGT ACAACAGTTT TCAGAGGAAA ATGCAAGCAA AATGAAGAGG 
ATCTATGGAG AATTCTGCAG CCATCACAAA GAAGCCATGA GTCTCTTTAA AGAGCTGCAG 
CAGAACAAGA AGTTTCAGAA TTTTATTAAG ATCCGAAATA GCAACCTGTT GGCTCGTCGC 
CGCGGAATCC CGGAGTGCAT CCTGCTAGTT ACCCAGCGCA TCACAAAGTA CCCAGTCTTG 
GTGGAAAGGA TCCTGCAGTA CACCAAGGAA AGGACTGAAG AGCACCGGGA CCTGTGCAAA 
GCTCTCGGCT TAATTAAAGA CATGATCGCA GCCGTGGACC TGAAAGTCAG CGAATACGAG 
AAGAACCAAA AATGGCTCGA GATCCTAAAT AAAATCGAGA ACAAAACATA CACAAAGCTC 
AAAAATGGCC ACGTGTTTAG AAAGCAGGCG CTGCTGAGCC AGGAACGGGC TCTCCTGCAT 
GATGGGCTTG TCTACTGGAA AACCGCTACG GGTCGCTTCA AAGACATCCT GGCTCTGCTA 
CTAACTGACG TGCTGCTCTT CTTACAAGAA AAAGACCAGA AATACATCTT TGCAGCTGTT 
GACCAGAAGC CATCAGTGAT TTCCCTCCAG AAGCTGATTG CTAGAGAAGT TGCCAACGAG 
GAGAGAGGCA TGTTTCTGAT CAGTGCTTCG TCAGCCGGCC CCGAGATGTA CGAAATCCAC 
ACCAATTCCA AGGAGGAGCG GAATAACTGG ATGAGGCGGA TCCAGCAGGC AGTAGAAAGT 
TGTCCAGAGG AAGAAGGAGG AAGGACGAGC GAGTCGGATG AAGAGAGGCG GAAAGCTGAA 
GCTAGAGTGG CCAAGATTCA GCAATGTCAA GAAATACTCA GTAACCAAGA CCAACAGATT 
TGCACGTACT TGGAAGAAAA GCTGCATATC TACGCTGAAC TTGGGGAACT GAGTGGCTTT 
GAGGATGTCC ACCTAGAACC CCATCTGCTC ATCAAACCTG ACCCTGGAGA GCCTCCCCAG 
GCAGCGTCCC TGCTTGCAGC AGCCCTGAGA GAAGCCGAGA GTCTGCAAGT GGCTGTGAAG 
GCTTCAAAGA TGGGCGATGT AAGCCAGTCA TCCGAAGAAA GCCCTGGAGG GACTGTCCTG 
ATGGACACAC CCAGTACACA GGATGTACCA GCGTCACCAA CTGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Arhgef28
Entrez Gene ID	110596
Full Name	Rho guanine nucleotide exchange factor (GEF) 28
Synonyms	9230110L08Rik,AI323540,D13Bwg1089e,RIP2,Rgnef,RhoGEF,Rhoip2,p190RhoGEF
General protein information	Preferred Names Rho guanine nucleotide exchange factor (GEF) 28 Names rho guanine nucleotide exchange factor 28 190 kDa guanine nucleotide exchange factor Rho interacting protein 2 Rho specific exchange factor Rho-guanine nucleotide exchange factor p190-RhoGEF rho-interacting protein 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 13 Map Location: 13 D1\|13 51.6 cM

Pan troglodytes (chimpanzee)	ARHGEF28	XP_001151239.2
Rattus norvegicus (Norway rat)	Arhgef28	NP_001102012.1
Gallus gallus (chicken)	LOC427201	XP_424789.4
Danio rerio (zebrafish)	arhgef28	XP_688428.4
Homo sapiens (human)	ARHGEF28	NP_001073948.2
Bos taurus (cattle)	RGNEF	NP_001095777.2
	ARHGEF28	XP_001101425.1
Canis lupus familiaris (dog)	ARHGEF28	XP_005617529.1
Mus musculus (house mouse)	Arhgef28	NP_036156.2
Xenopus tropicalis (tropical clawed frog)	arhgef28	XP_002934885.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Arhgef28 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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