Znf518b cDNA ORF clone, Mus caroli(Ryukyu mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Znf518b
Entrez Gene ID	110294707
Full Name	zinc finger protein 518B
Gene Type	protein-coding
Organism	Mus caroli(Ryukyu mouse)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021163113.1	XP_021018772.1	zinc finger protein 518B
XM_021163113.2	XP_021018772.1	zinc finger protein 518B

Pathways from BioSystems
Gene Ontology

The following Znf518b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Znf518b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMv01491	XM_021163113.2 Latest version!	Mus caroli zinc finger protein 518B (Znf518b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
OMv01491	XM_021163113.1	Mus caroli zinc finger protein 518B (Znf518b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Znf518b ( XM_021163113.1 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_021018772.1 Mus caroli zinc finger protein 518B (Znf518b), mRNA.

Price & Availability↑

CloneID	OMv01491
Clone ID Related Accession (Same CDS sequence)	XM_021163113.1 , XM_021163113.2
Accession Version	XM_021163113.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-20
Organism	Mus caroli(Ryukyu mouse)
Product	zinc finger protein 518B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034574.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus caroli Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGATAA GGGAGATGAA GGATATTGGA GAACAACTCT ATACCACACA AGTGAATGGG 
GGACCCAGTT CCTTGACCAT GTCCCCCAAA CAACCTAATA GAGCAACTCG GACGGAGAGA 
CAGGAAGCTC AGACCCTATT GTACCAAGGT TCAGAGGCAG AGGCTGCCAC AATGACAATT 
GCCACTTGTG TACAGTGCAA AAGTGTTCAC AAGATCCCCA CTCAAGACTT GAGGAAAGGC 
CCTGGGCAGA GCCAAGACAC TTACGTCTGC TTTAAGTGCA GCCTTCGAGC AGTGCCTACC 
CAGCTGCACT TTGTGAACAA CAACGCTGGT GCTGCGCATG TCAGAAATGA AACGGAAACC 
ATCTCGAGTC CTGTAAATAA ATTTAAGGTT AGGAACTTCA AGCCAGGCAA ATACTACTGT 
GATAAGTGTC GCTTCTCTAC AAAGGACCCG CTGCAGTACC GAAAGCACAC ACTGCAGCAC 
GAGGAGATCA AGTTCATCTG TTCCCACTGC AGCTACATCT CATACACGAA AGGGGAGTTC 
CAGAGGCATC TGGTGAAGCA CACGGGCATC TTCCCTTATC GCTGTGAGTA CTGCGACTAT 
GGTGCTATTC GCAATGACTA CATTGTCAAG CACAGGAGGA GGGTCCACGA GCGGGCTGGG 
GCCAAGCGGC CATTCAAAAC GGTGGCCAAG CTGGAACCCA AGAGAACCAG CATCTCAAAG 
CAAAGCATGG AACTCTCAAA GGCCCCCAGT CCCAGGGCTG CCTTCCAAAA CAAGTTGTCG 
GACCAGCTAT CTCGGTTCTC TCTCCATGCG AACAAAGACA AAACACACAA CCTGATGTTA 
TTACCCGAGC TAAAGAAGTA CCAAAAAGAT GTAGTGTGTA TTCCCAACAA AGTGACCCTG 
TCTGAGCCCA GGGAGGTGAG CCTGTTGGGG AACAAGAATG TTGAGGTTGA AGTGCTGTCC 
CCCTCAAAGG AACCTGTGCA TCCAGGGATG CCGCTGACTG TTATGGCACC CTCAGAGCTG 
GTGGTCCCCA CCAACTGTCT GGCCCAGTTG ATGGACGTGA AGGTTGTCAA TGGCGCTCAG 
CAGCTTGTGC TTAAACTGTT TCCGTTGGAA GAAAACGCCC GCCTGGAAAC GAGCCGAGGA 
GATGGAGGCA CTTCCGAGTG TCTGAGTACC GAGAAGGGCT TAGGGGAACA GAAAAAAAGG 
CTTTCTACAG AAGCATCGAG GTCACTAGCC GTGGAAGGGA ATGCTGGAGA CTTTGTGGGC 
CTGGATCGTC TGCACTCTTT GGTTCAGAAA CAGCTTAAAA ATGTGAAGTG GGTGAAGTCT 
TGCAATTTTT TCATGCCCAA TTCTGGTGTA CACAGTCACC AAGAATCTTT CCTCGGTTCC 
AATACAATCA AGGAGTTACA GAAATCTCCC AGTTTGTGTC CACCTAGAGC CCTTCCTTCT 
GCTGCCATAA AAAGTCACTC TCCAGCCTCT GTACAAAATA GTGTTTCCTA TGGTCCTGGG 
GCCACAGTGA GCCATTTCTT GAGTAAAGCA GCTGTTGCTT TTGCTGATGA CAGAAGAGGG 
GCCCGCAGTG ACTCCCAGCA GCTCCTGCCG CTTTCCTCGT TGCCTTCAAA GGTTCCCTTC 
TCTGGAGAGA AGGGCTTATT GCCTGTAGGT GAAAGTGACT TAGAAGCGAG GAATAGGATT 
AGTCGTCCTG AAACATTGGT TTCCTCTGAT AGGAAGCTGG AAGACAAGCA GATGGAAAGC 
AAGGCAGTGG GAAATACAGG CCAGGTTTCC TCTGTGCAGA ATAAAGAGTA TTTACACATA 
AACATAACTG GAGAAGAAAA AGCGAGGTCC CAACAGCCTG GGGATCAGCC TGTGCAGCCC 
AAGACTTCTG AAACAGCAGG TACCACATTT GAAGGCCCAA TCATCTCGTC AGTGTTTTCG 
CTGAGCTCTG GATCTGAAAA TGTGCCGGAG GGCATTAAAT GGAATAGTTC AACAACCAAA 
ATAAAGTCTA TTGAGCTGCT GCGCAGAAAG ATAGCTCAGT TGATTGAATC TTGTGGCAAG 
CCTTCCTCTT TGTCTGCCAA TAGTGCTCAT CGACGTTCCA TAGGTCAGGC ACCAAAGCTG 
ACTTCAAAAG CAACTCCCAA GGCTATTCAA GAGATGAGTG TGTCCCTTAC TGGCCCTGGC 
CCTACCCCTG GCCCTTCTGT GGGTCCTCTC CAAAAACCTC CAAACGAGGA CAGTATTACT 
GGCAGCAGAC AGCTTGTTCC ACAGCAAATC TGTCCCCAGT TTGTCAGTGC TAATGATGGA 
AAAATGGAAA ACAGAGTGAC CAGAAAGACT CCTGTTGCTA CTCCAGTGCT GATCCCCAAA 
GGGGCTGTGT TGAGGGTCCT TAACTCTTCT GAAGATGCTC ACATCATAGA GGCCACCTGT 
GACACACCTG TTAGCATACC TTGCAGCGAA GCCCAGTTAG CAGGAACACT TCCATTCTGC 
CCTATGCAAC AGACAGGCTC GGGCTCCCAG CCCTTGACAT GCAGAAGTGG AGCAGCAGAC 
ATGTCCCCAG GTCTGGAGAC ATCTCTTCGG CCCAAATCTA GAAAAGAGGA CCCAATCTAC 
AGTGCCACTG CTAAGAAAAT GGTCCCTGTG TACAGCACAG CACCAGGAAG CAGTGACTCC 
ATCAGGCAAG GAAGGCCAAT TTCTAGAAAC CTAACTGTAA GCAAGAATAA AACCAAGCAA 
GTGAACTCAG CCAAGAAGAA GAACAAGATG CAGGCCAATC CCGGCCGTTA TTTCAAAGAC 
CCCTCCTTTT TTCAGGTGGC AAGGCAGCTG CGCCTCATAG CTGCCAAACC AGATCAGTTG 
ATTAAATGTC CCCGTCGGAA CCAGCCTGTC ATTGTGCTAA ATCATCCTGA CGTTGACTCC 
CCCGAAGTGA CCAATGTGAT GAAGGTAATC AACAAGTACA AAGGCAATGT CCTCAAGGTT 
GTCCTGTCTG AAAGGACTAG GTGTCAGCTG GGTGTCCGGC GGTACCATAT GCGGCTGACC 
TACCAGAATG TGGCAGAAAC TAACCACATG AAGAGGCAGA TGATGTTGAA AATGAAACTG 
AAGAAGGTTC ATAAAAACAA CTATCAGGTG GTGGGCTCGT TGCCGGATGA CCCTGCCCAG 
TGTGTCTTTA AGTGCTGGTT CTGTGGGAGG CTCTACGAAG ACCAGGAAGA GTGGATGAGT 
CACGGCCAGA GGCATTTGAT AGAGGCCACC AGAGACTGGG ATGTCCTCTC TTCCAAGGGC 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021018772.1
CDS	344..3589
Translation	MQIREMKDIGEQLYTTQVNGGPSSLTMSPKQPNRATRTERQEAQTLLYQGSEAEAATMTIATCVQCKSVHKIPTQDLRKGPGQSQDTYVCFKCSLRAVPTQLHFVNNNAGAAHVRNETETISSPVNKFKVRNFKPGKYYCDKCRFSTKDPLQYRKHTLQHEEIKFICSHCSYISYTKGEFQRHLVKHTGIFPYRCEYCDYGAIRNDYIVKHRRRVHERAGAKRPFKTVAKLEPKRTSISKQSMELSKAPSPRAAFQNKLSDQLSRFSLHANKDKTHNLMLLPELKKYQKDVVCIPNKVTLSEPREVSLLGNKNVEVEVLSPSKEPVHPGMPLTVMAPSELVVPTNCLAQLMDVKVVNGAQQLVLKLFPLEENARLETSRGDGGTSECLSTEKGLGEQKKRLSTEASRSLAVEGNAGDFVGLDRLHSLVQKQLKNVKWVKSCNFFMPNSGVHSHQESFLGSNTIKELQKSPSLCPPRALPSAAIKSHSPASVQNSVSYGPGATVSHFLSKAAVAFADDRRGARSDSQQLLPLSSLPSKVPFSGEKGLLPVGESDLEARNRISRPETLVSSDRKLEDKQMESKAVGNTGQVSSVQNKEYLHINITGEEKARSQQPGDQPVQPKTSETAGTTFEGPIISSVFSLSSGSENVPEGIKWNSSTTKIKSIELLRRKIAQLIESCGKPSSLSANSAHRRSIGQAPKLTSKATPKAIQEMSVSLTGPGPTPGPSVGPLQKPPNEDSITGSRQLVPQQICPQFVSANDGKMENRVTRKTPVATPVLIPKGAVLRVLNSSEDAHIIEATCDTPVSIPCSEAQLAGTLPFCPMQQTGSGSQPLTCRSGAADMSPGLETSLRPKSRKEDPIYSATAKKMVPVYSTAPGSSDSIRQGRPISRNLTVSKNKTKQVNSAKKKNKMQANPGRYFKDPSFFQVARQLRLIAAKPDQLIKCPRRNQPVIVLNHPDVDSPEVTNVMKVINKYKGNVLKVVLSERTRCQLGVRRYHMRLTYQNVAETNHMKRQMMLKMKLKKVHKNNYQVVGSLPDDPAQCVFKCWFCGRLYEDQEEWMSHGQRHLIEATRDWDVLSSKGK

Target ORF information:

RefSeq Version	XM_021163113.1
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli zinc finger protein 518B (Znf518b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021163113.1

ORF Insert Sequence:

ATGCAGATAA GGGAGATGAA GGATATTGGA GAACAACTCT ATACCACACA AGTGAATGGG 
GGACCCAGTT CCTTGACCAT GTCCCCCAAA CAACCTAATA GAGCAACTCG GACGGAGAGA 
CAGGAAGCTC AGACCCTATT GTACCAAGGT TCAGAGGCAG AGGCTGCCAC AATGACAATT 
GCCACTTGTG TACAGTGCAA AAGTGTTCAC AAGATCCCCA CTCAAGACTT GAGGAAAGGC 
CCTGGGCAGA GCCAAGACAC TTACGTCTGC TTTAAGTGCA GCCTTCGAGC AGTGCCTACC 
CAGCTGCACT TTGTGAACAA CAACGCTGGT GCTGCGCATG TCAGAAATGA AACGGAAACC 
ATCTCGAGTC CTGTAAATAA ATTTAAGGTT AGGAACTTCA AGCCAGGCAA ATACTACTGT 
GATAAGTGTC GCTTCTCTAC AAAGGACCCG CTGCAGTACC GAAAGCACAC ACTGCAGCAC 
GAGGAGATCA AGTTCATCTG TTCCCACTGC AGCTACATCT CATACACGAA AGGGGAGTTC 
CAGAGGCATC TGGTGAAGCA CACGGGCATC TTCCCTTATC GCTGTGAGTA CTGCGACTAT 
GGTGCTATTC GCAATGACTA CATTGTCAAG CACAGGAGGA GGGTCCACGA GCGGGCTGGG 
GCCAAGCGGC CATTCAAAAC GGTGGCCAAG CTGGAACCCA AGAGAACCAG CATCTCAAAG 
CAAAGCATGG AACTCTCAAA GGCCCCCAGT CCCAGGGCTG CCTTCCAAAA CAAGTTGTCG 
GACCAGCTAT CTCGGTTCTC TCTCCATGCG AACAAAGACA AAACACACAA CCTGATGTTA 
TTACCCGAGC TAAAGAAGTA CCAAAAAGAT GTAGTGTGTA TTCCCAACAA AGTGACCCTG 
TCTGAGCCCA GGGAGGTGAG CCTGTTGGGG AACAAGAATG TTGAGGTTGA AGTGCTGTCC 
CCCTCAAAGG AACCTGTGCA TCCAGGGATG CCGCTGACTG TTATGGCACC CTCAGAGCTG 
GTGGTCCCCA CCAACTGTCT GGCCCAGTTG ATGGACGTGA AGGTTGTCAA TGGCGCTCAG 
CAGCTTGTGC TTAAACTGTT TCCGTTGGAA GAAAACGCCC GCCTGGAAAC GAGCCGAGGA 
GATGGAGGCA CTTCCGAGTG TCTGAGTACC GAGAAGGGCT TAGGGGAACA GAAAAAAAGG 
CTTTCTACAG AAGCATCGAG GTCACTAGCC GTGGAAGGGA ATGCTGGAGA CTTTGTGGGC 
CTGGATCGTC TGCACTCTTT GGTTCAGAAA CAGCTTAAAA ATGTGAAGTG GGTGAAGTCT 
TGCAATTTTT TCATGCCCAA TTCTGGTGTA CACAGTCACC AAGAATCTTT CCTCGGTTCC 
AATACAATCA AGGAGTTACA GAAATCTCCC AGTTTGTGTC CACCTAGAGC CCTTCCTTCT 
GCTGCCATAA AAAGTCACTC TCCAGCCTCT GTACAAAATA GTGTTTCCTA TGGTCCTGGG 
GCCACAGTGA GCCATTTCTT GAGTAAAGCA GCTGTTGCTT TTGCTGATGA CAGAAGAGGG 
GCCCGCAGTG ACTCCCAGCA GCTCCTGCCG CTTTCCTCGT TGCCTTCAAA GGTTCCCTTC 
TCTGGAGAGA AGGGCTTATT GCCTGTAGGT GAAAGTGACT TAGAAGCGAG GAATAGGATT 
AGTCGTCCTG AAACATTGGT TTCCTCTGAT AGGAAGCTGG AAGACAAGCA GATGGAAAGC 
AAGGCAGTGG GAAATACAGG CCAGGTTTCC TCTGTGCAGA ATAAAGAGTA TTTACACATA 
AACATAACTG GAGAAGAAAA AGCGAGGTCC CAACAGCCTG GGGATCAGCC TGTGCAGCCC 
AAGACTTCTG AAACAGCAGG TACCACATTT GAAGGCCCAA TCATCTCGTC AGTGTTTTCG 
CTGAGCTCTG GATCTGAAAA TGTGCCGGAG GGCATTAAAT GGAATAGTTC AACAACCAAA 
ATAAAGTCTA TTGAGCTGCT GCGCAGAAAG ATAGCTCAGT TGATTGAATC TTGTGGCAAG 
CCTTCCTCTT TGTCTGCCAA TAGTGCTCAT CGACGTTCCA TAGGTCAGGC ACCAAAGCTG 
ACTTCAAAAG CAACTCCCAA GGCTATTCAA GAGATGAGTG TGTCCCTTAC TGGCCCTGGC 
CCTACCCCTG GCCCTTCTGT GGGTCCTCTC CAAAAACCTC CAAACGAGGA CAGTATTACT 
GGCAGCAGAC AGCTTGTTCC ACAGCAAATC TGTCCCCAGT TTGTCAGTGC TAATGATGGA 
AAAATGGAAA ACAGAGTGAC CAGAAAGACT CCTGTTGCTA CTCCAGTGCT GATCCCCAAA 
GGGGCTGTGT TGAGGGTCCT TAACTCTTCT GAAGATGCTC ACATCATAGA GGCCACCTGT 
GACACACCTG TTAGCATACC TTGCAGCGAA GCCCAGTTAG CAGGAACACT TCCATTCTGC 
CCTATGCAAC AGACAGGCTC GGGCTCCCAG CCCTTGACAT GCAGAAGTGG AGCAGCAGAC 
ATGTCCCCAG GTCTGGAGAC ATCTCTTCGG CCCAAATCTA GAAAAGAGGA CCCAATCTAC 
AGTGCCACTG CTAAGAAAAT GGTCCCTGTG TACAGCACAG CACCAGGAAG CAGTGACTCC 
ATCAGGCAAG GAAGGCCAAT TTCTAGAAAC CTAACTGTAA GCAAGAATAA AACCAAGCAA 
GTGAACTCAG CCAAGAAGAA GAACAAGATG CAGGCCAATC CCGGCCGTTA TTTCAAAGAC 
CCCTCCTTTT TTCAGGTGGC AAGGCAGCTG CGCCTCATAG CTGCCAAACC AGATCAGTTG 
ATTAAATGTC CCCGTCGGAA CCAGCCTGTC ATTGTGCTAA ATCATCCTGA CGTTGACTCC 
CCCGAAGTGA CCAATGTGAT GAAGGTAATC AACAAGTACA AAGGCAATGT CCTCAAGGTT 
GTCCTGTCTG AAAGGACTAG GTGTCAGCTG GGTGTCCGGC GGTACCATAT GCGGCTGACC 
TACCAGAATG TGGCAGAAAC TAACCACATG AAGAGGCAGA TGATGTTGAA AATGAAACTG 
AAGAAGGTTC ATAAAAACAA CTATCAGGTG GTGGGCTCGT TGCCGGATGA CCCTGCCCAG 
TGTGTCTTTA AGTGCTGGTT CTGTGGGAGG CTCTACGAAG ACCAGGAAGA GTGGATGAGT 
CACGGCCAGA GGCATTTGAT AGAGGCCACC AGAGACTGGG ATGTCCTCTC TTCCAAGGGC 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Znf518b ( XM_021163113.2 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_021018772.1 Mus caroli zinc finger protein 518B (Znf518b), mRNA.

Price & Availability↑

CloneID	OMv01491
Clone ID Related Accession (Same CDS sequence)	XM_021163113.1 , XM_021163113.2
Accession Version	XM_021163113.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-09
Organism	Mus caroli(Ryukyu mouse)
Product	zinc finger protein 518B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034574.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 10, 2019 this sequence version replaced XM_021163113.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus caroli Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGATAA GGGAGATGAA GGATATTGGA GAACAACTCT ATACCACACA AGTGAATGGG 
GGACCCAGTT CCTTGACCAT GTCCCCCAAA CAACCTAATA GAGCAACTCG GACGGAGAGA 
CAGGAAGCTC AGACCCTATT GTACCAAGGT TCAGAGGCAG AGGCTGCCAC AATGACAATT 
GCCACTTGTG TACAGTGCAA AAGTGTTCAC AAGATCCCCA CTCAAGACTT GAGGAAAGGC 
CCTGGGCAGA GCCAAGACAC TTACGTCTGC TTTAAGTGCA GCCTTCGAGC AGTGCCTACC 
CAGCTGCACT TTGTGAACAA CAACGCTGGT GCTGCGCATG TCAGAAATGA AACGGAAACC 
ATCTCGAGTC CTGTAAATAA ATTTAAGGTT AGGAACTTCA AGCCAGGCAA ATACTACTGT 
GATAAGTGTC GCTTCTCTAC AAAGGACCCG CTGCAGTACC GAAAGCACAC ACTGCAGCAC 
GAGGAGATCA AGTTCATCTG TTCCCACTGC AGCTACATCT CATACACGAA AGGGGAGTTC 
CAGAGGCATC TGGTGAAGCA CACGGGCATC TTCCCTTATC GCTGTGAGTA CTGCGACTAT 
GGTGCTATTC GCAATGACTA CATTGTCAAG CACAGGAGGA GGGTCCACGA GCGGGCTGGG 
GCCAAGCGGC CATTCAAAAC GGTGGCCAAG CTGGAACCCA AGAGAACCAG CATCTCAAAG 
CAAAGCATGG AACTCTCAAA GGCCCCCAGT CCCAGGGCTG CCTTCCAAAA CAAGTTGTCG 
GACCAGCTAT CTCGGTTCTC TCTCCATGCG AACAAAGACA AAACACACAA CCTGATGTTA 
TTACCCGAGC TAAAGAAGTA CCAAAAAGAT GTAGTGTGTA TTCCCAACAA AGTGACCCTG 
TCTGAGCCCA GGGAGGTGAG CCTGTTGGGG AACAAGAATG TTGAGGTTGA AGTGCTGTCC 
CCCTCAAAGG AACCTGTGCA TCCAGGGATG CCGCTGACTG TTATGGCACC CTCAGAGCTG 
GTGGTCCCCA CCAACTGTCT GGCCCAGTTG ATGGACGTGA AGGTTGTCAA TGGCGCTCAG 
CAGCTTGTGC TTAAACTGTT TCCGTTGGAA GAAAACGCCC GCCTGGAAAC GAGCCGAGGA 
GATGGAGGCA CTTCCGAGTG TCTGAGTACC GAGAAGGGCT TAGGGGAACA GAAAAAAAGG 
CTTTCTACAG AAGCATCGAG GTCACTAGCC GTGGAAGGGA ATGCTGGAGA CTTTGTGGGC 
CTGGATCGTC TGCACTCTTT GGTTCAGAAA CAGCTTAAAA ATGTGAAGTG GGTGAAGTCT 
TGCAATTTTT TCATGCCCAA TTCTGGTGTA CACAGTCACC AAGAATCTTT CCTCGGTTCC 
AATACAATCA AGGAGTTACA GAAATCTCCC AGTTTGTGTC CACCTAGAGC CCTTCCTTCT 
GCTGCCATAA AAAGTCACTC TCCAGCCTCT GTACAAAATA GTGTTTCCTA TGGTCCTGGG 
GCCACAGTGA GCCATTTCTT GAGTAAAGCA GCTGTTGCTT TTGCTGATGA CAGAAGAGGG 
GCCCGCAGTG ACTCCCAGCA GCTCCTGCCG CTTTCCTCGT TGCCTTCAAA GGTTCCCTTC 
TCTGGAGAGA AGGGCTTATT GCCTGTAGGT GAAAGTGACT TAGAAGCGAG GAATAGGATT 
AGTCGTCCTG AAACATTGGT TTCCTCTGAT AGGAAGCTGG AAGACAAGCA GATGGAAAGC 
AAGGCAGTGG GAAATACAGG CCAGGTTTCC TCTGTGCAGA ATAAAGAGTA TTTACACATA 
AACATAACTG GAGAAGAAAA AGCGAGGTCC CAACAGCCTG GGGATCAGCC TGTGCAGCCC 
AAGACTTCTG AAACAGCAGG TACCACATTT GAAGGCCCAA TCATCTCGTC AGTGTTTTCG 
CTGAGCTCTG GATCTGAAAA TGTGCCGGAG GGCATTAAAT GGAATAGTTC AACAACCAAA 
ATAAAGTCTA TTGAGCTGCT GCGCAGAAAG ATAGCTCAGT TGATTGAATC TTGTGGCAAG 
CCTTCCTCTT TGTCTGCCAA TAGTGCTCAT CGACGTTCCA TAGGTCAGGC ACCAAAGCTG 
ACTTCAAAAG CAACTCCCAA GGCTATTCAA GAGATGAGTG TGTCCCTTAC TGGCCCTGGC 
CCTACCCCTG GCCCTTCTGT GGGTCCTCTC CAAAAACCTC CAAACGAGGA CAGTATTACT 
GGCAGCAGAC AGCTTGTTCC ACAGCAAATC TGTCCCCAGT TTGTCAGTGC TAATGATGGA 
AAAATGGAAA ACAGAGTGAC CAGAAAGACT CCTGTTGCTA CTCCAGTGCT GATCCCCAAA 
GGGGCTGTGT TGAGGGTCCT TAACTCTTCT GAAGATGCTC ACATCATAGA GGCCACCTGT 
GACACACCTG TTAGCATACC TTGCAGCGAA GCCCAGTTAG CAGGAACACT TCCATTCTGC 
CCTATGCAAC AGACAGGCTC GGGCTCCCAG CCCTTGACAT GCAGAAGTGG AGCAGCAGAC 
ATGTCCCCAG GTCTGGAGAC ATCTCTTCGG CCCAAATCTA GAAAAGAGGA CCCAATCTAC 
AGTGCCACTG CTAAGAAAAT GGTCCCTGTG TACAGCACAG CACCAGGAAG CAGTGACTCC 
ATCAGGCAAG GAAGGCCAAT TTCTAGAAAC CTAACTGTAA GCAAGAATAA AACCAAGCAA 
GTGAACTCAG CCAAGAAGAA GAACAAGATG CAGGCCAATC CCGGCCGTTA TTTCAAAGAC 
CCCTCCTTTT TTCAGGTGGC AAGGCAGCTG CGCCTCATAG CTGCCAAACC AGATCAGTTG 
ATTAAATGTC CCCGTCGGAA CCAGCCTGTC ATTGTGCTAA ATCATCCTGA CGTTGACTCC 
CCCGAAGTGA CCAATGTGAT GAAGGTAATC AACAAGTACA AAGGCAATGT CCTCAAGGTT 
GTCCTGTCTG AAAGGACTAG GTGTCAGCTG GGTGTCCGGC GGTACCATAT GCGGCTGACC 
TACCAGAATG TGGCAGAAAC TAACCACATG AAGAGGCAGA TGATGTTGAA AATGAAACTG 
AAGAAGGTTC ATAAAAACAA CTATCAGGTG GTGGGCTCGT TGCCGGATGA CCCTGCCCAG 
TGTGTCTTTA AGTGCTGGTT CTGTGGGAGG CTCTACGAAG ACCAGGAAGA GTGGATGAGT 
CACGGCCAGA GGCATTTGAT AGAGGCCACC AGAGACTGGG ATGTCCTCTC TTCCAAGGGC 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021018772.1
CDS	576..3821
Translation	MQIREMKDIGEQLYTTQVNGGPSSLTMSPKQPNRATRTERQEAQTLLYQGSEAEAATMTIATCVQCKSVHKIPTQDLRKGPGQSQDTYVCFKCSLRAVPTQLHFVNNNAGAAHVRNETETISSPVNKFKVRNFKPGKYYCDKCRFSTKDPLQYRKHTLQHEEIKFICSHCSYISYTKGEFQRHLVKHTGIFPYRCEYCDYGAIRNDYIVKHRRRVHERAGAKRPFKTVAKLEPKRTSISKQSMELSKAPSPRAAFQNKLSDQLSRFSLHANKDKTHNLMLLPELKKYQKDVVCIPNKVTLSEPREVSLLGNKNVEVEVLSPSKEPVHPGMPLTVMAPSELVVPTNCLAQLMDVKVVNGAQQLVLKLFPLEENARLETSRGDGGTSECLSTEKGLGEQKKRLSTEASRSLAVEGNAGDFVGLDRLHSLVQKQLKNVKWVKSCNFFMPNSGVHSHQESFLGSNTIKELQKSPSLCPPRALPSAAIKSHSPASVQNSVSYGPGATVSHFLSKAAVAFADDRRGARSDSQQLLPLSSLPSKVPFSGEKGLLPVGESDLEARNRISRPETLVSSDRKLEDKQMESKAVGNTGQVSSVQNKEYLHINITGEEKARSQQPGDQPVQPKTSETAGTTFEGPIISSVFSLSSGSENVPEGIKWNSSTTKIKSIELLRRKIAQLIESCGKPSSLSANSAHRRSIGQAPKLTSKATPKAIQEMSVSLTGPGPTPGPSVGPLQKPPNEDSITGSRQLVPQQICPQFVSANDGKMENRVTRKTPVATPVLIPKGAVLRVLNSSEDAHIIEATCDTPVSIPCSEAQLAGTLPFCPMQQTGSGSQPLTCRSGAADMSPGLETSLRPKSRKEDPIYSATAKKMVPVYSTAPGSSDSIRQGRPISRNLTVSKNKTKQVNSAKKKNKMQANPGRYFKDPSFFQVARQLRLIAAKPDQLIKCPRRNQPVIVLNHPDVDSPEVTNVMKVINKYKGNVLKVVLSERTRCQLGVRRYHMRLTYQNVAETNHMKRQMMLKMKLKKVHKNNYQVVGSLPDDPAQCVFKCWFCGRLYEDQEEWMSHGQRHLIEATRDWDVLSSKGK

Target ORF information:

RefSeq Version	XM_021163113.2
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli zinc finger protein 518B (Znf518b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021163113.2

ORF Insert Sequence:

ATGCAGATAA GGGAGATGAA GGATATTGGA GAACAACTCT ATACCACACA AGTGAATGGG 
GGACCCAGTT CCTTGACCAT GTCCCCCAAA CAACCTAATA GAGCAACTCG GACGGAGAGA 
CAGGAAGCTC AGACCCTATT GTACCAAGGT TCAGAGGCAG AGGCTGCCAC AATGACAATT 
GCCACTTGTG TACAGTGCAA AAGTGTTCAC AAGATCCCCA CTCAAGACTT GAGGAAAGGC 
CCTGGGCAGA GCCAAGACAC TTACGTCTGC TTTAAGTGCA GCCTTCGAGC AGTGCCTACC 
CAGCTGCACT TTGTGAACAA CAACGCTGGT GCTGCGCATG TCAGAAATGA AACGGAAACC 
ATCTCGAGTC CTGTAAATAA ATTTAAGGTT AGGAACTTCA AGCCAGGCAA ATACTACTGT 
GATAAGTGTC GCTTCTCTAC AAAGGACCCG CTGCAGTACC GAAAGCACAC ACTGCAGCAC 
GAGGAGATCA AGTTCATCTG TTCCCACTGC AGCTACATCT CATACACGAA AGGGGAGTTC 
CAGAGGCATC TGGTGAAGCA CACGGGCATC TTCCCTTATC GCTGTGAGTA CTGCGACTAT 
GGTGCTATTC GCAATGACTA CATTGTCAAG CACAGGAGGA GGGTCCACGA GCGGGCTGGG 
GCCAAGCGGC CATTCAAAAC GGTGGCCAAG CTGGAACCCA AGAGAACCAG CATCTCAAAG 
CAAAGCATGG AACTCTCAAA GGCCCCCAGT CCCAGGGCTG CCTTCCAAAA CAAGTTGTCG 
GACCAGCTAT CTCGGTTCTC TCTCCATGCG AACAAAGACA AAACACACAA CCTGATGTTA 
TTACCCGAGC TAAAGAAGTA CCAAAAAGAT GTAGTGTGTA TTCCCAACAA AGTGACCCTG 
TCTGAGCCCA GGGAGGTGAG CCTGTTGGGG AACAAGAATG TTGAGGTTGA AGTGCTGTCC 
CCCTCAAAGG AACCTGTGCA TCCAGGGATG CCGCTGACTG TTATGGCACC CTCAGAGCTG 
GTGGTCCCCA CCAACTGTCT GGCCCAGTTG ATGGACGTGA AGGTTGTCAA TGGCGCTCAG 
CAGCTTGTGC TTAAACTGTT TCCGTTGGAA GAAAACGCCC GCCTGGAAAC GAGCCGAGGA 
GATGGAGGCA CTTCCGAGTG TCTGAGTACC GAGAAGGGCT TAGGGGAACA GAAAAAAAGG 
CTTTCTACAG AAGCATCGAG GTCACTAGCC GTGGAAGGGA ATGCTGGAGA CTTTGTGGGC 
CTGGATCGTC TGCACTCTTT GGTTCAGAAA CAGCTTAAAA ATGTGAAGTG GGTGAAGTCT 
TGCAATTTTT TCATGCCCAA TTCTGGTGTA CACAGTCACC AAGAATCTTT CCTCGGTTCC 
AATACAATCA AGGAGTTACA GAAATCTCCC AGTTTGTGTC CACCTAGAGC CCTTCCTTCT 
GCTGCCATAA AAAGTCACTC TCCAGCCTCT GTACAAAATA GTGTTTCCTA TGGTCCTGGG 
GCCACAGTGA GCCATTTCTT GAGTAAAGCA GCTGTTGCTT TTGCTGATGA CAGAAGAGGG 
GCCCGCAGTG ACTCCCAGCA GCTCCTGCCG CTTTCCTCGT TGCCTTCAAA GGTTCCCTTC 
TCTGGAGAGA AGGGCTTATT GCCTGTAGGT GAAAGTGACT TAGAAGCGAG GAATAGGATT 
AGTCGTCCTG AAACATTGGT TTCCTCTGAT AGGAAGCTGG AAGACAAGCA GATGGAAAGC 
AAGGCAGTGG GAAATACAGG CCAGGTTTCC TCTGTGCAGA ATAAAGAGTA TTTACACATA 
AACATAACTG GAGAAGAAAA AGCGAGGTCC CAACAGCCTG GGGATCAGCC TGTGCAGCCC 
AAGACTTCTG AAACAGCAGG TACCACATTT GAAGGCCCAA TCATCTCGTC AGTGTTTTCG 
CTGAGCTCTG GATCTGAAAA TGTGCCGGAG GGCATTAAAT GGAATAGTTC AACAACCAAA 
ATAAAGTCTA TTGAGCTGCT GCGCAGAAAG ATAGCTCAGT TGATTGAATC TTGTGGCAAG 
CCTTCCTCTT TGTCTGCCAA TAGTGCTCAT CGACGTTCCA TAGGTCAGGC ACCAAAGCTG 
ACTTCAAAAG CAACTCCCAA GGCTATTCAA GAGATGAGTG TGTCCCTTAC TGGCCCTGGC 
CCTACCCCTG GCCCTTCTGT GGGTCCTCTC CAAAAACCTC CAAACGAGGA CAGTATTACT 
GGCAGCAGAC AGCTTGTTCC ACAGCAAATC TGTCCCCAGT TTGTCAGTGC TAATGATGGA 
AAAATGGAAA ACAGAGTGAC CAGAAAGACT CCTGTTGCTA CTCCAGTGCT GATCCCCAAA 
GGGGCTGTGT TGAGGGTCCT TAACTCTTCT GAAGATGCTC ACATCATAGA GGCCACCTGT 
GACACACCTG TTAGCATACC TTGCAGCGAA GCCCAGTTAG CAGGAACACT TCCATTCTGC 
CCTATGCAAC AGACAGGCTC GGGCTCCCAG CCCTTGACAT GCAGAAGTGG AGCAGCAGAC 
ATGTCCCCAG GTCTGGAGAC ATCTCTTCGG CCCAAATCTA GAAAAGAGGA CCCAATCTAC 
AGTGCCACTG CTAAGAAAAT GGTCCCTGTG TACAGCACAG CACCAGGAAG CAGTGACTCC 
ATCAGGCAAG GAAGGCCAAT TTCTAGAAAC CTAACTGTAA GCAAGAATAA AACCAAGCAA 
GTGAACTCAG CCAAGAAGAA GAACAAGATG CAGGCCAATC CCGGCCGTTA TTTCAAAGAC 
CCCTCCTTTT TTCAGGTGGC AAGGCAGCTG CGCCTCATAG CTGCCAAACC AGATCAGTTG 
ATTAAATGTC CCCGTCGGAA CCAGCCTGTC ATTGTGCTAA ATCATCCTGA CGTTGACTCC 
CCCGAAGTGA CCAATGTGAT GAAGGTAATC AACAAGTACA AAGGCAATGT CCTCAAGGTT 
GTCCTGTCTG AAAGGACTAG GTGTCAGCTG GGTGTCCGGC GGTACCATAT GCGGCTGACC 
TACCAGAATG TGGCAGAAAC TAACCACATG AAGAGGCAGA TGATGTTGAA AATGAAACTG 
AAGAAGGTTC ATAAAAACAA CTATCAGGTG GTGGGCTCGT TGCCGGATGA CCCTGCCCAG 
TGTGTCTTTA AGTGCTGGTT CTGTGGGAGG CTCTACGAAG ACCAGGAAGA GTGGATGAGT 
CACGGCCAGA GGCATTTGAT AGAGGCCACC AGAGACTGGG ATGTCCTCTC TTCCAAGGGC 
AAATGA