CRIM1 cDNA ORF clone, Miniopterus natalensis

Gene
Clones
gRNAs

Summary

Gene Symbol	CRIM1
Entrez Gene ID	107541800
Full Name	cysteine rich transmembrane BMP regulator 1
Gene Type	protein-coding
Organism	Miniopterus natalensis

mRNA and Protein(s)

mRNA	Protein	Name
XM_016218972.1	XP_016074458.1	cysteine-rich motor neuron 1 protein isoform X2
XM_016218971.1	XP_016074457.1	cysteine-rich motor neuron 1 protein isoform X1

Pathways from BioSystems
Gene Ontology

The following CRIM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CRIM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMi49029	XM_016218971.1 Latest version!	Miniopterus natalensis cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OMi49030	XM_016218972.1 Latest version!	Miniopterus natalensis cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CRIM1 ( XM_016218971.1 ) cDNA ORF clone, Miniopterus natalensis -> XP_016074457.1 Miniopterus natalensis cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMi49029
Clone ID Related Accession (Same CDS sequence)	XM_016218971.1
Accession Version	XM_016218971.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-10
Organism	Miniopterus natalensis
Product	cysteine-rich motor neuron 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015504737.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Miniopterus natalensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAATCT TGACATCCTT ACACATTTTT GCTTTCCAGC AGGCCTTCCT TCGAAGGAAG 
CTAACAAGCA GACATCATGT GGTTTGTAAT TGTTTTGCAG ATGAGAACTG GGACGATGAC 
CAGCTGCTTG GTTTTGAACC CTGTAAAGAA AACCTCGTCT TTGGCTGCAA TATAATCAAT 
GGGAAGTGCG AGTGTGTCAC CGTTCGGACC TGCAACAATC CCTTTGAGTT TCCGAGGAAG 
GATATGTGCT TTGCCGCTTT AAAGAGGATT CAAGAAGAGA AGCCAGATTG CTCCAAGGCC 
CGCTGTGAAG TCCAGTTCTC TCCGCGCTGT CCCGAAGATT CCGTTCTGAT CGAGGGCTAC 
GCTCCCCCCG GGAAGTGCTG CCCCTTACCC AGCCGCTGCG TGTGCGACCC TGCGGGCTGC 
CTGCGCAAAG TCTGCCAGCC GGGATTCCTG AACATACTAG TGTCAAAGGC CTCGGGGAAG 
CCGGGAGAGT GTTGTGACCT GTATGAGTGC AAACCAGTGT CCAGCGTGGA CTGCAGCACT 
GTGGAGTGCC CCACCGTTCA GCAGGCCGTG TGCCCCCTGG ATAGCTACGA AACTCAAGTC 
AGACTCACAG CGGACAGTTG CTGCTCTCTG CCAACAAGAT GCGAGTGTCT CTCTGGCTTA 
TGTGGTTTCC CCGTGTGTGA GGTGGGATCC ATTCCCCGCA TAGTCTCTCG TGGAGATGGG 
ACACCTGGAA AGTGCTGTGA TGTCTTTGAA TGTGTTAATG AAACGAAGCC AGCCTGCGTG 
TTCAACAACG TGGAATATAA CGACGGGGAT ATGTTCCGGT TGGACAAGTG CCGGTTCTGC 
CGGTGCCAGG GTGGCGTCGC CATCTGCTTC ACCGCCCAGT GCGGGGAGCT GAGCTGCGAG 
CGGTACTACG TGCCCGAGGG GGAGTGCTGC CCGGTGTGCG AAGATCCGGT GTATCCATTC 
AATAATCCTG CTGGTTGCTA CGCCAACGGG CAGATCCGCG CCCATGGAGA CCGGTGGCGG 
GAAGACGACT GCACGTTCTG CCAGTGCATC AATGGGGAGC CCCACTGCGT GGCCACGGTC 
TGCGGGCAGA GCTGCATGAA CCCCGTCAAG GTGCCCGGGG AGTGCTGCCC CGTGTGCGAA 
GAGCCCACCT ACATCACAAT CGATCCGCCG GCGTGCGGGG AGCTAGCCAA CTGCAGCCTG 
ACCGAGAAGG ACTGTATTTA CGGTTTCAAG CTTGACCACA GTGGCTGTCG AACTTGTCAG 
TGCAAAAGCA GGGAGGAGCT GTGCGCCGGC CTCAAGCGAG GCTGCACGCT GGACTGTCCC 
TTCGGGTTCC TGACTGACGC CCACAACTGC GAGATCTGTC GGTGCCGGCC CCGGCCCAAG 
AAGTGCCGAC CCGTAACCTG TGACAAGTAC TGTCCATTCG GATACCTGAA GAATAAGCAC 
GGCTGTGACG TCTGTCGCTG TAGGAAGTGC CCAGAGCTCC CATGCAGTAA AAACTGCTCC 
TTGGGCTTCC AGAAGGACAG CCATGGCTGT GTCACCTGCA AGTGCAGAGA GGTCCCCACA 
TCACCCAGCT CGCCTGCCCT GTCAGGCACA TGTCTGTCCA TGGATGGCCA TCACCATAAA 
AATGAGGAGA GCTGGCATGA TGGGTGCCGG GAGTGCTACT GTCACAATGG CCGGGAGATG 
TGTGCTCTGA TCACCTGCCC GGTGCCCGCC TGTGGCAGCC CCACCATCCG TCCAGGACAG 
TGCTGCCCGT CATGTTCAGA TGACCTTGTG GTGCAGAAGC CAGAGCTCAG CAACCCCTCC 
ATTTGCCACG CCCCTGGAGG AGAGTACTTC GTGGAAGGAG AAACGTGGAA CATAGACTCC 
TGTACACAGT GCACCTGCCA CAGCGGGCGG GTGATGTGTG AGACAGAGGT GTGCCCGCCG 
CTGCTCTGCC AGAACCCTTC GCGCACGCAG GACTCCTGCT GCCCGCAGTG CACAGATGCA 
CCCCTGCAGC CCGCCGCGCC CCGTAACGAC AGCGTGCCGA GCTACTGCAA GAACGATGAA 
GGAGACATAT TCCTGGCGGC TGAGTCCTGG AAGCCTGACG TCTGCACCAG CTGCGTGTGC 
ATGAATAGCC TCATTAGCTG CTACTCCGAG TCCTGCCCTT CAGTGTCCTG TGAAAGACCT 
GTTTTGCGAA AAGGCCAGTG TTGTCCCTAC TGCATAGAGG ACACGGTCGC GAAGAAGGCG 
GCGTGCCACT TCAGCGGGCG GGCCTACGCC GACGAGGAGC GCTGGGACCT AGACAGCTGC 
ACCCACTGCT ACTGCCTGCA GGGCCAGACG CTCTGCTCCA CCGTCAGCTG CCCGCCGCTG 
CCCTGCCTGG AGCCCATCAA CGTGGGAGGG AGCTGCTGCC CCATCTGCCC AGAAATGTAT 
GTCCCAGAAC CAACGAATGT GCCCATTGAG AAGGTAAATC ATCGTGGAGA GACTGACCGA 
GAGGTTCCCG TGTGGCCCAC TCCCAGTGAA AATGACATTG TCCATCTCCC CAGAGACATG 
GGTCACCTCC AGGTAGATTA CAGAGATAAC AGCAGGCCGC ACGCAGGCGA GGATGCCTCG 
CTGGACAGCA TCGCCTCGGT CGTGGTTCCC ATCGTGATAG GCCTCTCCAT GATCGTGGCC 
TTCCTGTTCG TCAATCAGAA GAAGCAGTGG ATACCCCTGC TTTGCTGGTA CCGAACGCCG 
ACTAAGCCTT CTTCCTTAAA TAACCAGCTT GTGTCTGTGG ACTGCAAGAA AGGAACCCGA 
GCCCCGGGGG ACAGTGCGCA GAGGATGCTC AGAATTGCTG AGCCGGACGC GAGATTCAGC 
GGCTTCTACA GCATGCAGAA GCAGAGCCAC CTGCAGGCGG ACAACTTCTA CCAGACGGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016074457.1
CDS	623..3505
Translation	MLILTSLHIFAFQQAFLRRKLTSRHHVVCNCFADENWDDDQLLGFEPCKENLVFGCNIINGKCECVTVRTCNNPFEFPRKDMCFAALKRIQEEKPDCSKARCEVQFSPRCPEDSVLIEGYAPPGKCCPLPSRCVCDPAGCLRKVCQPGFLNILVSKASGKPGECCDLYECKPVSSVDCSTVECPTVQQAVCPLDSYETQVRLTADSCCSLPTRCECLSGLCGFPVCEVGSIPRIVSRGDGTPGKCCDVFECVNETKPACVFNNVEYNDGDMFRLDKCRFCRCQGGVAICFTAQCGELSCERYYVPEGECCPVCEDPVYPFNNPAGCYANGQIRAHGDRWREDDCTFCQCINGEPHCVATVCGQSCMNPVKVPGECCPVCEEPTYITIDPPACGELANCSLTEKDCIYGFKLDHSGCRTCQCKSREELCAGLKRGCTLDCPFGFLTDAHNCEICRCRPRPKKCRPVTCDKYCPFGYLKNKHGCDVCRCRKCPELPCSKNCSLGFQKDSHGCVTCKCREVPTSPSSPALSGTCLSMDGHHHKNEESWHDGCRECYCHNGREMCALITCPVPACGSPTIRPGQCCPSCSDDLVVQKPELSNPSICHAPGGEYFVEGETWNIDSCTQCTCHSGRVMCETEVCPPLLCQNPSRTQDSCCPQCTDAPLQPAAPRNDSVPSYCKNDEGDIFLAAESWKPDVCTSCVCMNSLISCYSESCPSVSCERPVLRKGQCCPYCIEDTVAKKAACHFSGRAYADEERWDLDSCTHCYCLQGQTLCSTVSCPPLPCLEPINVGGSCCPICPEMYVPEPTNVPIEKVNHRGETDREVPVWPTPSENDIVHLPRDMGHLQVDYRDNSRPHAGEDASLDSIASVVVPIVIGLSMIVAFLFVNQKKQWIPLLCWYRTPTKPSSLNNQLVSVDCKKGTRAPGDSAQRMLRIAEPDARFSGFYSMQKQSHLQADNFYQTV

Target ORF information:

RefSeq Version	XM_016218971.1
Organism	Miniopterus natalensis
Definition	Miniopterus natalensis cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016218971.1

ORF Insert Sequence:

ATGTTAATCT TGACATCCTT ACACATTTTT GCTTTCCAGC AGGCCTTCCT TCGAAGGAAG 
CTAACAAGCA GACATCATGT GGTTTGTAAT TGTTTTGCAG ATGAGAACTG GGACGATGAC 
CAGCTGCTTG GTTTTGAACC CTGTAAAGAA AACCTCGTCT TTGGCTGCAA TATAATCAAT 
GGGAAGTGCG AGTGTGTCAC CGTTCGGACC TGCAACAATC CCTTTGAGTT TCCGAGGAAG 
GATATGTGCT TTGCCGCTTT AAAGAGGATT CAAGAAGAGA AGCCAGATTG CTCCAAGGCC 
CGCTGTGAAG TCCAGTTCTC TCCGCGCTGT CCCGAAGATT CCGTTCTGAT CGAGGGCTAC 
GCTCCCCCCG GGAAGTGCTG CCCCTTACCC AGCCGCTGCG TGTGCGACCC TGCGGGCTGC 
CTGCGCAAAG TCTGCCAGCC GGGATTCCTG AACATACTAG TGTCAAAGGC CTCGGGGAAG 
CCGGGAGAGT GTTGTGACCT GTATGAGTGC AAACCAGTGT CCAGCGTGGA CTGCAGCACT 
GTGGAGTGCC CCACCGTTCA GCAGGCCGTG TGCCCCCTGG ATAGCTACGA AACTCAAGTC 
AGACTCACAG CGGACAGTTG CTGCTCTCTG CCAACAAGAT GCGAGTGTCT CTCTGGCTTA 
TGTGGTTTCC CCGTGTGTGA GGTGGGATCC ATTCCCCGCA TAGTCTCTCG TGGAGATGGG 
ACACCTGGAA AGTGCTGTGA TGTCTTTGAA TGTGTTAATG AAACGAAGCC AGCCTGCGTG 
TTCAACAACG TGGAATATAA CGACGGGGAT ATGTTCCGGT TGGACAAGTG CCGGTTCTGC 
CGGTGCCAGG GTGGCGTCGC CATCTGCTTC ACCGCCCAGT GCGGGGAGCT GAGCTGCGAG 
CGGTACTACG TGCCCGAGGG GGAGTGCTGC CCGGTGTGCG AAGATCCGGT GTATCCATTC 
AATAATCCTG CTGGTTGCTA CGCCAACGGG CAGATCCGCG CCCATGGAGA CCGGTGGCGG 
GAAGACGACT GCACGTTCTG CCAGTGCATC AATGGGGAGC CCCACTGCGT GGCCACGGTC 
TGCGGGCAGA GCTGCATGAA CCCCGTCAAG GTGCCCGGGG AGTGCTGCCC CGTGTGCGAA 
GAGCCCACCT ACATCACAAT CGATCCGCCG GCGTGCGGGG AGCTAGCCAA CTGCAGCCTG 
ACCGAGAAGG ACTGTATTTA CGGTTTCAAG CTTGACCACA GTGGCTGTCG AACTTGTCAG 
TGCAAAAGCA GGGAGGAGCT GTGCGCCGGC CTCAAGCGAG GCTGCACGCT GGACTGTCCC 
TTCGGGTTCC TGACTGACGC CCACAACTGC GAGATCTGTC GGTGCCGGCC CCGGCCCAAG 
AAGTGCCGAC CCGTAACCTG TGACAAGTAC TGTCCATTCG GATACCTGAA GAATAAGCAC 
GGCTGTGACG TCTGTCGCTG TAGGAAGTGC CCAGAGCTCC CATGCAGTAA AAACTGCTCC 
TTGGGCTTCC AGAAGGACAG CCATGGCTGT GTCACCTGCA AGTGCAGAGA GGTCCCCACA 
TCACCCAGCT CGCCTGCCCT GTCAGGCACA TGTCTGTCCA TGGATGGCCA TCACCATAAA 
AATGAGGAGA GCTGGCATGA TGGGTGCCGG GAGTGCTACT GTCACAATGG CCGGGAGATG 
TGTGCTCTGA TCACCTGCCC GGTGCCCGCC TGTGGCAGCC CCACCATCCG TCCAGGACAG 
TGCTGCCCGT CATGTTCAGA TGACCTTGTG GTGCAGAAGC CAGAGCTCAG CAACCCCTCC 
ATTTGCCACG CCCCTGGAGG AGAGTACTTC GTGGAAGGAG AAACGTGGAA CATAGACTCC 
TGTACACAGT GCACCTGCCA CAGCGGGCGG GTGATGTGTG AGACAGAGGT GTGCCCGCCG 
CTGCTCTGCC AGAACCCTTC GCGCACGCAG GACTCCTGCT GCCCGCAGTG CACAGATGCA 
CCCCTGCAGC CCGCCGCGCC CCGTAACGAC AGCGTGCCGA GCTACTGCAA GAACGATGAA 
GGAGACATAT TCCTGGCGGC TGAGTCCTGG AAGCCTGACG TCTGCACCAG CTGCGTGTGC 
ATGAATAGCC TCATTAGCTG CTACTCCGAG TCCTGCCCTT CAGTGTCCTG TGAAAGACCT 
GTTTTGCGAA AAGGCCAGTG TTGTCCCTAC TGCATAGAGG ACACGGTCGC GAAGAAGGCG 
GCGTGCCACT TCAGCGGGCG GGCCTACGCC GACGAGGAGC GCTGGGACCT AGACAGCTGC 
ACCCACTGCT ACTGCCTGCA GGGCCAGACG CTCTGCTCCA CCGTCAGCTG CCCGCCGCTG 
CCCTGCCTGG AGCCCATCAA CGTGGGAGGG AGCTGCTGCC CCATCTGCCC AGAAATGTAT 
GTCCCAGAAC CAACGAATGT GCCCATTGAG AAGGTAAATC ATCGTGGAGA GACTGACCGA 
GAGGTTCCCG TGTGGCCCAC TCCCAGTGAA AATGACATTG TCCATCTCCC CAGAGACATG 
GGTCACCTCC AGGTAGATTA CAGAGATAAC AGCAGGCCGC ACGCAGGCGA GGATGCCTCG 
CTGGACAGCA TCGCCTCGGT CGTGGTTCCC ATCGTGATAG GCCTCTCCAT GATCGTGGCC 
TTCCTGTTCG TCAATCAGAA GAAGCAGTGG ATACCCCTGC TTTGCTGGTA CCGAACGCCG 
ACTAAGCCTT CTTCCTTAAA TAACCAGCTT GTGTCTGTGG ACTGCAAGAA AGGAACCCGA 
GCCCCGGGGG ACAGTGCGCA GAGGATGCTC AGAATTGCTG AGCCGGACGC GAGATTCAGC 
GGCTTCTACA GCATGCAGAA GCAGAGCCAC CTGCAGGCGG ACAACTTCTA CCAGACGGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRIM1 ( XM_016218972.1 ) cDNA ORF clone, Miniopterus natalensis -> XP_016074458.1 Miniopterus natalensis cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMi49030
Clone ID Related Accession (Same CDS sequence)	XM_016218972.1
Accession Version	XM_016218972.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2565bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-10
Organism	Miniopterus natalensis
Product	cysteine-rich motor neuron 1 protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015504737.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Miniopterus natalensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAATCT TGACATCCTT ACACATTTTT GCTTTCCAGC AGGCCTTCCT TCGAAGGAAG 
CTAACAAGCA GACATCATGT GGTTTGTAAT TGTTTTGCAG ATGAGAACTG GGACGATGAC 
CAGCTGCTTG GTTTTGAACC CTGTAAAGAA AACCTCGTCT TTGGCTGCAA TATAATCAAT 
GGGAAGTGCG AGTGTGTCAC CGTTCGGACC TGCAACAATC CCTTTGAGTT TCCGAGGAAG 
GATATGTGCT TTGCCGCTTT AAAGAGGATT CAAGAAGAGA AGCCAGATTG CTCCAAGGCC 
CGCTGTGAAG TCCAGTTCTC TCCGCGCTGT CCCGAAGATT CCGTTCTGAT CGAGGGCTAC 
GCTCCCCCCG GGAAGTGCTG CCCCTTACCC AGCCGCTGCG TGTGCGACCC TGCGGGCTGC 
CTGCGCAAAG TCTGCCAGCC GGGATTCCTG AACATACTAG TGTCAAAGGC CTCGGGGAAG 
CCGGGAGAGT GTTGTGACCT GTATGAGTGC AAACCAGTGT CCAGCGTGGA CTGCAGCACT 
GTGGAGTGCC CCACCGTTCA GCAGGCCGTG TGCCCCCTGG ATAGCTACGA AACTCAAGTC 
AGACTCACAG CGGACAGTTG CTGCTCTCTG CCAACAAGAT GCGAGTGTCT CTCTGGCTTA 
TGTGGTTTCC CCGTGTGTGA GGTGGGATCC ATTCCCCGCA TAGTCTCTCG TGGAGATGGG 
ACACCTGGAA AGTGCTGTGA TGTCTTTGAA TGTGTTAATG AAACGAAGCC AGCCTGCGTG 
TTCAACAACG TGGAATATAA CGACGGGGAT ATGTTCCGGT TGGACAAGTG CCGGTTCTGC 
CGGTGCCAGG GTGGCGTCGC CATCTGCTTC ACCGCCCAGT GCGGGGAGCT GAGCTGCGAG 
CGGTACTACG TGCCCGAGGG GGAGTGCTGC CCGGTGTGCG AAGATCCGGT GTATCCATTC 
AATAATCCTG CTGGTTGCTA CGCCAACGGG CAGATCCGCG CCCATGGAGA CCGGTGGCGG 
GAAGACGACT GCACGTTCTG CCAGTGCATC AATGGGGAGC CCCACTGCGT GGCCACGGTC 
TGCGGGCAGA GCTGCATGAA CCCCGTCAAG GTGCCCGGGG AGTGCTGCCC CGTGTGCGAA 
GAGCCCACCT ACATCACAAT CGATCCGCCG GCGTGCGGGG AGCTAGCCAA CTGCAGCCTG 
ACCGAGAAGG ACTGTATTTA CGGTTTCAAG CTTGACCACA GTGGCTGTCG AACTTGTCAG 
TGCAAAAGCA GGGAGGAGCT GTGCGCCGGC CTCAAGCGAG GCTGCACGCT GGACTGTCCC 
TTCGGGTTCC TGACTGACGC CCACAACTGC GAGATCTGTC GGTGCCGGCC CCGGCCCAAG 
AAGTGCCGAC CCGTAACCTG TGACAAGTAC TGTCCATTCG GATACCTGAA GAATAAGCAC 
GGCTGTGACG TCTGTCGCTG TAGGAAGTGC CCAGAGCTCC CATGCAGTAA AAACTGCTCC 
TTGGGCTTCC AGAAGGACAG CCATGGCTGT GTCACCTGCA AGTGCAGAGA GGTCCCCACA 
TCACCCAGCT CGCCTGCCCT GTCAGGCACA TGTCTGTCCA TGGATGGCCA TCACCATAAA 
AATGAGGAGA GCTGGCATGA TGGGTGCCGG GAGTGCTACT GTCACAATGG CCGGGAGATG 
TGTGCTCTGA TCACCTGCCC GGTGCCCGCC TGTGGCAGCC CCACCATCCG TCCAGGACAG 
TGCTGCCCGT CATGTTCAGA TGACCTTGTG GTGCAGAAGC CAGAGCTCAG CAACCCCTCC 
ATTTGCCACG CCCCTGGAGG AGAGTACTTC GTGGAAGGAG AAACGTGGAA CATAGACTCC 
TGTACACAGT GCACCTGCCA CAGCGGGCGG GTGATGTGTG AGACAGAGGT GTGCCCGCCG 
CTGCTCTGCC AGAACCCTTC GCGCACGCAG GACTCCTGCT GCCCGCAGTG CACAGATGCA 
CCCCTGCAGC CCGCCGCGCC CCGTAACGAC AGCGTGCCGA GCTACTGCAA GAACGATGAA 
GGAGACATAT TCCTGGCGGC TGAGTCCTGG AAGCCTGACG TCTGCACCAG CTGCGTGTGC 
ATGAATAGCC TCATTAGCTG CTACTCCGAG TCCTGCCCTT CAGTGTCCTG TGAAAGACCT 
GTTTTGCGAA AAGGCCAGTG TTGTCCCTAC TGCATAGACA TGGGTCACCT CCAGGTAGAT 
TACAGAGATA ACAGCAGGCC GCACGCAGGC GAGGATGCCT CGCTGGACAG CATCGCCTCG 
GTCGTGGTTC CCATCGTGAT AGGCCTCTCC ATGATCGTGG CCTTCCTGTT CGTCAATCAG 
AAGAAGCAGT GGATACCCCT GCTTTGCTGG TACCGAACGC CGACTAAGCC TTCTTCCTTA 
AATAACCAGC TTGTGTCTGT GGACTGCAAG AAAGGAACCC GAGCCCCGGG GGACAGTGCG 
CAGAGGATGC TCAGAATTGC TGAGCCGGAC GCGAGATTCA GCGGCTTCTA CAGCATGCAG 
AAGCAGAGCC ACCTGCAGGC GGACAACTTC TACCAGACGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016074458.1
CDS	619..3183
Translation	MLILTSLHIFAFQQAFLRRKLTSRHHVVCNCFADENWDDDQLLGFEPCKENLVFGCNIINGKCECVTVRTCNNPFEFPRKDMCFAALKRIQEEKPDCSKARCEVQFSPRCPEDSVLIEGYAPPGKCCPLPSRCVCDPAGCLRKVCQPGFLNILVSKASGKPGECCDLYECKPVSSVDCSTVECPTVQQAVCPLDSYETQVRLTADSCCSLPTRCECLSGLCGFPVCEVGSIPRIVSRGDGTPGKCCDVFECVNETKPACVFNNVEYNDGDMFRLDKCRFCRCQGGVAICFTAQCGELSCERYYVPEGECCPVCEDPVYPFNNPAGCYANGQIRAHGDRWREDDCTFCQCINGEPHCVATVCGQSCMNPVKVPGECCPVCEEPTYITIDPPACGELANCSLTEKDCIYGFKLDHSGCRTCQCKSREELCAGLKRGCTLDCPFGFLTDAHNCEICRCRPRPKKCRPVTCDKYCPFGYLKNKHGCDVCRCRKCPELPCSKNCSLGFQKDSHGCVTCKCREVPTSPSSPALSGTCLSMDGHHHKNEESWHDGCRECYCHNGREMCALITCPVPACGSPTIRPGQCCPSCSDDLVVQKPELSNPSICHAPGGEYFVEGETWNIDSCTQCTCHSGRVMCETEVCPPLLCQNPSRTQDSCCPQCTDAPLQPAAPRNDSVPSYCKNDEGDIFLAAESWKPDVCTSCVCMNSLISCYSESCPSVSCERPVLRKGQCCPYCIDMGHLQVDYRDNSRPHAGEDASLDSIASVVVPIVIGLSMIVAFLFVNQKKQWIPLLCWYRTPTKPSSLNNQLVSVDCKKGTRAPGDSAQRMLRIAEPDARFSGFYSMQKQSHLQADNFYQTV

Target ORF information:

RefSeq Version	XM_016218972.1
Organism	Miniopterus natalensis
Definition	Miniopterus natalensis cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016218972.1

ORF Insert Sequence:

ATGTTAATCT TGACATCCTT ACACATTTTT GCTTTCCAGC AGGCCTTCCT TCGAAGGAAG 
CTAACAAGCA GACATCATGT GGTTTGTAAT TGTTTTGCAG ATGAGAACTG GGACGATGAC 
CAGCTGCTTG GTTTTGAACC CTGTAAAGAA AACCTCGTCT TTGGCTGCAA TATAATCAAT 
GGGAAGTGCG AGTGTGTCAC CGTTCGGACC TGCAACAATC CCTTTGAGTT TCCGAGGAAG 
GATATGTGCT TTGCCGCTTT AAAGAGGATT CAAGAAGAGA AGCCAGATTG CTCCAAGGCC 
CGCTGTGAAG TCCAGTTCTC TCCGCGCTGT CCCGAAGATT CCGTTCTGAT CGAGGGCTAC 
GCTCCCCCCG GGAAGTGCTG CCCCTTACCC AGCCGCTGCG TGTGCGACCC TGCGGGCTGC 
CTGCGCAAAG TCTGCCAGCC GGGATTCCTG AACATACTAG TGTCAAAGGC CTCGGGGAAG 
CCGGGAGAGT GTTGTGACCT GTATGAGTGC AAACCAGTGT CCAGCGTGGA CTGCAGCACT 
GTGGAGTGCC CCACCGTTCA GCAGGCCGTG TGCCCCCTGG ATAGCTACGA AACTCAAGTC 
AGACTCACAG CGGACAGTTG CTGCTCTCTG CCAACAAGAT GCGAGTGTCT CTCTGGCTTA 
TGTGGTTTCC CCGTGTGTGA GGTGGGATCC ATTCCCCGCA TAGTCTCTCG TGGAGATGGG 
ACACCTGGAA AGTGCTGTGA TGTCTTTGAA TGTGTTAATG AAACGAAGCC AGCCTGCGTG 
TTCAACAACG TGGAATATAA CGACGGGGAT ATGTTCCGGT TGGACAAGTG CCGGTTCTGC 
CGGTGCCAGG GTGGCGTCGC CATCTGCTTC ACCGCCCAGT GCGGGGAGCT GAGCTGCGAG 
CGGTACTACG TGCCCGAGGG GGAGTGCTGC CCGGTGTGCG AAGATCCGGT GTATCCATTC 
AATAATCCTG CTGGTTGCTA CGCCAACGGG CAGATCCGCG CCCATGGAGA CCGGTGGCGG 
GAAGACGACT GCACGTTCTG CCAGTGCATC AATGGGGAGC CCCACTGCGT GGCCACGGTC 
TGCGGGCAGA GCTGCATGAA CCCCGTCAAG GTGCCCGGGG AGTGCTGCCC CGTGTGCGAA 
GAGCCCACCT ACATCACAAT CGATCCGCCG GCGTGCGGGG AGCTAGCCAA CTGCAGCCTG 
ACCGAGAAGG ACTGTATTTA CGGTTTCAAG CTTGACCACA GTGGCTGTCG AACTTGTCAG 
TGCAAAAGCA GGGAGGAGCT GTGCGCCGGC CTCAAGCGAG GCTGCACGCT GGACTGTCCC 
TTCGGGTTCC TGACTGACGC CCACAACTGC GAGATCTGTC GGTGCCGGCC CCGGCCCAAG 
AAGTGCCGAC CCGTAACCTG TGACAAGTAC TGTCCATTCG GATACCTGAA GAATAAGCAC 
GGCTGTGACG TCTGTCGCTG TAGGAAGTGC CCAGAGCTCC CATGCAGTAA AAACTGCTCC 
TTGGGCTTCC AGAAGGACAG CCATGGCTGT GTCACCTGCA AGTGCAGAGA GGTCCCCACA 
TCACCCAGCT CGCCTGCCCT GTCAGGCACA TGTCTGTCCA TGGATGGCCA TCACCATAAA 
AATGAGGAGA GCTGGCATGA TGGGTGCCGG GAGTGCTACT GTCACAATGG CCGGGAGATG 
TGTGCTCTGA TCACCTGCCC GGTGCCCGCC TGTGGCAGCC CCACCATCCG TCCAGGACAG 
TGCTGCCCGT CATGTTCAGA TGACCTTGTG GTGCAGAAGC CAGAGCTCAG CAACCCCTCC 
ATTTGCCACG CCCCTGGAGG AGAGTACTTC GTGGAAGGAG AAACGTGGAA CATAGACTCC 
TGTACACAGT GCACCTGCCA CAGCGGGCGG GTGATGTGTG AGACAGAGGT GTGCCCGCCG 
CTGCTCTGCC AGAACCCTTC GCGCACGCAG GACTCCTGCT GCCCGCAGTG CACAGATGCA 
CCCCTGCAGC CCGCCGCGCC CCGTAACGAC AGCGTGCCGA GCTACTGCAA GAACGATGAA 
GGAGACATAT TCCTGGCGGC TGAGTCCTGG AAGCCTGACG TCTGCACCAG CTGCGTGTGC 
ATGAATAGCC TCATTAGCTG CTACTCCGAG TCCTGCCCTT CAGTGTCCTG TGAAAGACCT 
GTTTTGCGAA AAGGCCAGTG TTGTCCCTAC TGCATAGACA TGGGTCACCT CCAGGTAGAT 
TACAGAGATA ACAGCAGGCC GCACGCAGGC GAGGATGCCT CGCTGGACAG CATCGCCTCG 
GTCGTGGTTC CCATCGTGAT AGGCCTCTCC ATGATCGTGG CCTTCCTGTT CGTCAATCAG 
AAGAAGCAGT GGATACCCCT GCTTTGCTGG TACCGAACGC CGACTAAGCC TTCTTCCTTA 
AATAACCAGC TTGTGTCTGT GGACTGCAAG AAAGGAACCC GAGCCCCGGG GGACAGTGCG 
CAGAGGATGC TCAGAATTGC TGAGCCGGAC GCGAGATTCA GCGGCTTCTA CAGCATGCAG 
AAGCAGAGCC ACCTGCAGGC GGACAACTTC TACCAGACGG TGTGA