ANKRD35 cDNA ORF clone, Microcebus murinus(gray mouse lemur)

Gene
Clones
gRNAs

Summary

Gene Symbol	ANKRD35
Entrez Gene ID	105869827
Full Name	ankyrin repeat domain 35
Gene Type	protein-coding
Organism	Microcebus murinus(gray mouse lemur)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012761931.2	XP_012617385.2	ankyrin repeat domain-containing protein 35 isoform X1
XM_012761932.2	XP_012617386.2	ankyrin repeat domain-containing protein 35 isoform X2

Pathways from BioSystems
Gene Ontology

The following ANKRD35 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ANKRD35 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMi73072	XM_012761931.2 Latest version!	Microcebus murinus ankyrin repeat domain 35 (ANKRD35), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMi73073	XM_012761932.2 Latest version!	Microcebus murinus ankyrin repeat domain 35 (ANKRD35), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ANKRD35 ( XM_012761931.2 ) cDNA ORF clone, Microcebus murinus(gray mouse lemur) -> XP_012617385.2 Microcebus murinus ankyrin repeat domain 35 (ANKRD35), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMi73072
Clone ID Related Accession (Same CDS sequence)	XM_012761931.2
Accession Version	XM_012761931.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-23
Organism	Microcebus murinus(gray mouse lemur)
Product	ankyrin repeat domain-containing protein 35 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033661.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 24, 2017 this sequence version replaced XM_012761931.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microcebus murinus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACAGT TGAGAAAAGC GAAGCCCACC CGGGAAGCCG GGACCGTCGG CCTCCAGCCG 
AGGCGCGCCG AGGGGCGGGC GGAGGCGGCG TTTGGGCGGA TGCCGCCCGG GAGCTGCTGC 
TGGCGGGCGC AGTCGCGGGA GGAGTCTCGG CCGGAGCCGG CCCGGGGCCG CTCGGCAGCG 
AGCAGCCGCC TCCGAGTTCG GTGGGTTTGG GAACCTGTGG CCCGGCTCTC TGCGCGTCCC 
CGTCCCCCCG TGGCCCTGAC CCCGGCCATG AAGCGCATCT TCTCCTGCTC CAGCTCACAG 
GTGGCGGTGG AGAGATGGAA TCGTCGTGAC CAGAAGCTGC TGGAGGCAGT GCAGCGGGGG 
GACGTGGGAC GCGTGGCTGC CCTTGCCTCC AGGAAGTCGG CCCGGCCCAC CAAGCTAGAC 
TCCAATGGCC AGTCCCCGTT TCATCTGGCA GCCTCCAAAG GCCTGACGGA GTGTCTGACT 
ATACTGCTTG CAAATGGGGC TGACATCAAC AGCAAGAATG AGGATGGAAG TACTGCCTTG 
CACTTGGCCA CCATCTCCTG CCAGCCACAG TGTGTCAAGG TTCTGCTTCA GCATGGTGCT 
AATGAAGATG CTGTGGATGC AGAAAACCGT AGTCCATTGC ACTGGGCAGC AATCCTTCAA 
CCCTCCCCAG CCTCCTCTGG CTGTGCCTCA AGTGTGCTCC TGCTTTGTGA CCACGAAGCC 
TTCCTGGACG TGTTGGATAA TGATGGACGC ACACCCCTGA TGATCGCGTC GCTGGGCGGT 
CACGCGGCTA TCTGCTCACA GTTGCTGCAG AGAGGCGCCC GAGTCAACGT TACAGACAAG 
GATGACAAGT CGGCTTTGAT CCTGGCCTGT GAGAAAGGCA GTGCTGAGGT GGCAGAACTG 
CTCCTAGGCC ACGGAGCAGA CGCAGGGGCA ATGGACAGAA CAGGGCACGA TGCTCTCCAT 
TATGCTTTGC ACACGCAGGA CAAGGCACTG TGGAGGCTAC TACGGCAGGC CTTGAACCGG 
CGGCGGAGGG GCGGCGGTCA AAGGCTAGTC CAATACCCTG ATCTTGCATC TCAGGTCTCT 
CCATCTGAGC CCCAGATGGG TTCTCCACCT AAGAGCCCAT GGAGAGCGGA GCCTGAGGAG 
GAACAGGAGG AAGAGGATGA TGAAGATCCA TGCTCAGAGG AGTGGAAGTG GAAGTATGAA 
GAGGAGCAGA GGAAAGTTTC TCAGTTGGAG CAGGAGCTGG TGTGCAAAAC AGAGGAGTGT 
AAGACTCAAG CTGCAGCCTA CCTGGGCCTA GAGAACCAGA TTCGAGAGCA GGTGAAGGAG 
CTGGGGCTCC TCCTATCCCA AGAGCCTGCA GCTTCAGGAA GGCGAGGCTC TAGTCTCAGG 
CTTGGAGGGG ATGGCATGGA GCAGGGTTGT CCCCTGGACC TGCTGGTGGA GCGTATACAA 
GAGCTGAAGA AGCAGCAGCA GGTAGCAGCC ACAGTCAACC CAACATTAGC TCCCAGGAAA 
GCTGAGGATT CAGCTCCAGG AGAGGTTCAA TATGAAGTCC ATGGAAGGTT CCAGCCACAA 
GAACAGGGGC CACTCCAGAG CCCAAGGTCT GTGACCATTG GGAAAACCAC AGAACAGCAA 
CTGACCACCA ATGGGGGACA TACCCTTGGC CTTGATCATC CTAACCAGTT GCCTGCTGGC 
CAGAAGGAGA GGCCCCAGGC CCCAGGGGTG GAACCAGCAG GCACAGTGGT TGAACAAGTG 
GGCACAGCAG CCATGAACCA GCTTCTGCTA CAACTAAGGG AGGAGCTGGC TGCAGTTTGG 
CGAGAAAAGG ATGCTGCCCG GGGAGCTTTG TCAAGACCAG TCCTAGAGGG AGCCCTGGGG 
ACTCCCCGGG CTGAGGCTGC AGCAGCTGCC TGGGAGAAGA TGGAGGCCCG GTTGGAGCGG 
GTGCTCGTGA GGCTGGATCG GGCAAAGGCA GGACTACAGG TGAAACCTGC CCAGGAGTCC 
AGAGAGGGAG CCCCAAAGGC AGCCCCAGGG AGCATCAGAG AGAATGAAGA GGAGAAGAGG 
TCTCCTGGGG CTCGAGGAGA ACCTCTAGGG GCCCCTGGAG GAGAACAGGC CCCAGGAGGA 
GGCCTGCCAA AGGGACAGCT GGAGAAGGAA GTGTCAGCAC TGAGACTGAG CAACAGTAAC 
TTGCTGGAGG AGTTGGGGGA GCTGGGCCGG GAGCGCCAGA GGTTGCAGAG GGAGCTGCAG 
TCCCTGAGCC AGCGGCTGCA ACGCGAGTTT GTGCCCAAGC CAGAGGCGCA GGTCCAGCTG 
CAGCAGTTGC GGCAGAGCGT GGGGCTACTG ACAGATGAAC TGGCCATGGA GAAAGAGGCC 
ACAGAGAAGC TGCGGAAGCG CCTGGCCTCC CAGAGCAGTG GCCTTCGAGG GCTGTGGGAC 
TGCCTGCCCC CAGATCTAGT GGGCAAGGGG AGCACACGGA GCACGCTGGC CGAGCCCCTG 
GAGGAGCTGC AGGGCTGCAT CAGCACCCTG GTGGACCGGC ACCGCGAGGC CCAGCAGGTG 
CTGGCTCGGC TGGAGGAGGA AAACCAGCAG CTGCGGGGGT CCCTGGCTCC TGGTGGGGAG 
CCAGGCATCT CCTCAAGGGC CCCGGCATCC CCCCAAGTGG CTGCTCTGGA GCAAGACCTA 
GGGAAGCTGG AGGAGGAGCT GCGGGTGGTT CAGGCCACGA TGAGCGGGAA GAGCCAGGAG 
ATCGGGAAGC TGAAGCAGCT GCTCTACCAA GCCACAGAGG AGGTGGCAGA GCTGAGGGCC 
CGGGAGGCTG CCAGCCTACG GCAACATGAG AAAACTCGGG GTTCGCTGGT GGCCCAGGCT 
CAGGCTTGGG GTCAAGAGCT AAAGGCCCTG CTGGAAAAAT ATAACACAGC CTGCCAGGAG 
ATGGGTCGGC TGCGGGAGGC CATGGCAGAG GAGCGCCGCA GGAACGGGGA GCTGGCTGCT 
CAGGCCGCTG AGCAGGAGCA GCAGGCCAGA GAGATGCGCG GGCGGTCTGA GCAGTTTGAG 
AAAACAGCGG AGCTGCTGAA AGAGAAGATG GAGCATCTCA TTGGGGCTTG CCGGGACAAG 
GAGGCCAAGA TCAAGGAGCT GTTGAAGAAG CTGGAGCAGC TTTCAGAGGA GGTTCTAGCA 
GTTCGGGGAG AAAATGCTCG CCTTGTCCTG CAGCTGCAGG ATTACCAGAA GAACCACGAA 
GAGATCATCT CTACTTATAG GAATCATCTG CTGAATGCTG CTCGGGGCTA CATGGAACAG 
GATGTGTATG ATATCCTACT GCGAATCCTC AGCATACAAG AGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012617385.2
CDS	16..3303
Translation	MRQLRKAKPTREAGTVGLQPRRAEGRAEAAFGRMPPGSCCWRAQSREESRPEPARGRSAASSRLRVRWVWEPVARLSARPRPPVALTPAMKRIFSCSSSQVAVERWNRRDQKLLEAVQRGDVGRVAALASRKSARPTKLDSNGQSPFHLAASKGLTECLTILLANGADINSKNEDGSTALHLATISCQPQCVKVLLQHGANEDAVDAENRSPLHWAAILQPSPASSGCASSVLLLCDHEAFLDVLDNDGRTPLMIASLGGHAAICSQLLQRGARVNVTDKDDKSALILACEKGSAEVAELLLGHGADAGAMDRTGHDALHYALHTQDKALWRLLRQALNRRRRGGGQRLVQYPDLASQVSPSEPQMGSPPKSPWRAEPEEEQEEEDDEDPCSEEWKWKYEEEQRKVSQLEQELVCKTEECKTQAAAYLGLENQIREQVKELGLLLSQEPAASGRRGSSLRLGGDGMEQGCPLDLLVERIQELKKQQQVAATVNPTLAPRKAEDSAPGEVQYEVHGRFQPQEQGPLQSPRSVTIGKTTEQQLTTNGGHTLGLDHPNQLPAGQKERPQAPGVEPAGTVVEQVGTAAMNQLLLQLREELAAVWREKDAARGALSRPVLEGALGTPRAEAAAAAWEKMEARLERVLVRLDRAKAGLQVKPAQESREGAPKAAPGSIRENEEEKRSPGARGEPLGAPGGEQAPGGGLPKGQLEKEVSALRLSNSNLLEELGELGRERQRLQRELQSLSQRLQREFVPKPEAQVQLQQLRQSVGLLTDELAMEKEATEKLRKRLASQSSGLRGLWDCLPPDLVGKGSTRSTLAEPLEELQGCISTLVDRHREAQQVLARLEEENQQLRGSLAPGGEPGISSRAPASPQVAALEQDLGKLEEELRVVQATMSGKSQEIGKLKQLLYQATEEVAELRAREAASLRQHEKTRGSLVAQAQAWGQELKALLEKYNTACQEMGRLREAMAEERRRNGELAAQAAEQEQQAREMRGRSEQFEKTAELLKEKMEHLIGACRDKEAKIKELLKKLEQLSEEVLAVRGENARLVLQLQDYQKNHEEIISTYRNHLLNAARGYMEQDVYDILLRILSIQEE

Target ORF information:

RefSeq Version	XM_012761931.2
Organism	Microcebus murinus(gray mouse lemur)
Definition	Microcebus murinus ankyrin repeat domain 35 (ANKRD35), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012761931.2

ORF Insert Sequence:

ATGCGACAGT TGAGAAAAGC GAAGCCCACC CGGGAAGCCG GGACCGTCGG CCTCCAGCCG 
AGGCGCGCCG AGGGGCGGGC GGAGGCGGCG TTTGGGCGGA TGCCGCCCGG GAGCTGCTGC 
TGGCGGGCGC AGTCGCGGGA GGAGTCTCGG CCGGAGCCGG CCCGGGGCCG CTCGGCAGCG 
AGCAGCCGCC TCCGAGTTCG GTGGGTTTGG GAACCTGTGG CCCGGCTCTC TGCGCGTCCC 
CGTCCCCCCG TGGCCCTGAC CCCGGCCATG AAGCGCATCT TCTCCTGCTC CAGCTCACAG 
GTGGCGGTGG AGAGATGGAA TCGTCGTGAC CAGAAGCTGC TGGAGGCAGT GCAGCGGGGG 
GACGTGGGAC GCGTGGCTGC CCTTGCCTCC AGGAAGTCGG CCCGGCCCAC CAAGCTAGAC 
TCCAATGGCC AGTCCCCGTT TCATCTGGCA GCCTCCAAAG GCCTGACGGA GTGTCTGACT 
ATACTGCTTG CAAATGGGGC TGACATCAAC AGCAAGAATG AGGATGGAAG TACTGCCTTG 
CACTTGGCCA CCATCTCCTG CCAGCCACAG TGTGTCAAGG TTCTGCTTCA GCATGGTGCT 
AATGAAGATG CTGTGGATGC AGAAAACCGT AGTCCATTGC ACTGGGCAGC AATCCTTCAA 
CCCTCCCCAG CCTCCTCTGG CTGTGCCTCA AGTGTGCTCC TGCTTTGTGA CCACGAAGCC 
TTCCTGGACG TGTTGGATAA TGATGGACGC ACACCCCTGA TGATCGCGTC GCTGGGCGGT 
CACGCGGCTA TCTGCTCACA GTTGCTGCAG AGAGGCGCCC GAGTCAACGT TACAGACAAG 
GATGACAAGT CGGCTTTGAT CCTGGCCTGT GAGAAAGGCA GTGCTGAGGT GGCAGAACTG 
CTCCTAGGCC ACGGAGCAGA CGCAGGGGCA ATGGACAGAA CAGGGCACGA TGCTCTCCAT 
TATGCTTTGC ACACGCAGGA CAAGGCACTG TGGAGGCTAC TACGGCAGGC CTTGAACCGG 
CGGCGGAGGG GCGGCGGTCA AAGGCTAGTC CAATACCCTG ATCTTGCATC TCAGGTCTCT 
CCATCTGAGC CCCAGATGGG TTCTCCACCT AAGAGCCCAT GGAGAGCGGA GCCTGAGGAG 
GAACAGGAGG AAGAGGATGA TGAAGATCCA TGCTCAGAGG AGTGGAAGTG GAAGTATGAA 
GAGGAGCAGA GGAAAGTTTC TCAGTTGGAG CAGGAGCTGG TGTGCAAAAC AGAGGAGTGT 
AAGACTCAAG CTGCAGCCTA CCTGGGCCTA GAGAACCAGA TTCGAGAGCA GGTGAAGGAG 
CTGGGGCTCC TCCTATCCCA AGAGCCTGCA GCTTCAGGAA GGCGAGGCTC TAGTCTCAGG 
CTTGGAGGGG ATGGCATGGA GCAGGGTTGT CCCCTGGACC TGCTGGTGGA GCGTATACAA 
GAGCTGAAGA AGCAGCAGCA GGTAGCAGCC ACAGTCAACC CAACATTAGC TCCCAGGAAA 
GCTGAGGATT CAGCTCCAGG AGAGGTTCAA TATGAAGTCC ATGGAAGGTT CCAGCCACAA 
GAACAGGGGC CACTCCAGAG CCCAAGGTCT GTGACCATTG GGAAAACCAC AGAACAGCAA 
CTGACCACCA ATGGGGGACA TACCCTTGGC CTTGATCATC CTAACCAGTT GCCTGCTGGC 
CAGAAGGAGA GGCCCCAGGC CCCAGGGGTG GAACCAGCAG GCACAGTGGT TGAACAAGTG 
GGCACAGCAG CCATGAACCA GCTTCTGCTA CAACTAAGGG AGGAGCTGGC TGCAGTTTGG 
CGAGAAAAGG ATGCTGCCCG GGGAGCTTTG TCAAGACCAG TCCTAGAGGG AGCCCTGGGG 
ACTCCCCGGG CTGAGGCTGC AGCAGCTGCC TGGGAGAAGA TGGAGGCCCG GTTGGAGCGG 
GTGCTCGTGA GGCTGGATCG GGCAAAGGCA GGACTACAGG TGAAACCTGC CCAGGAGTCC 
AGAGAGGGAG CCCCAAAGGC AGCCCCAGGG AGCATCAGAG AGAATGAAGA GGAGAAGAGG 
TCTCCTGGGG CTCGAGGAGA ACCTCTAGGG GCCCCTGGAG GAGAACAGGC CCCAGGAGGA 
GGCCTGCCAA AGGGACAGCT GGAGAAGGAA GTGTCAGCAC TGAGACTGAG CAACAGTAAC 
TTGCTGGAGG AGTTGGGGGA GCTGGGCCGG GAGCGCCAGA GGTTGCAGAG GGAGCTGCAG 
TCCCTGAGCC AGCGGCTGCA ACGCGAGTTT GTGCCCAAGC CAGAGGCGCA GGTCCAGCTG 
CAGCAGTTGC GGCAGAGCGT GGGGCTACTG ACAGATGAAC TGGCCATGGA GAAAGAGGCC 
ACAGAGAAGC TGCGGAAGCG CCTGGCCTCC CAGAGCAGTG GCCTTCGAGG GCTGTGGGAC 
TGCCTGCCCC CAGATCTAGT GGGCAAGGGG AGCACACGGA GCACGCTGGC CGAGCCCCTG 
GAGGAGCTGC AGGGCTGCAT CAGCACCCTG GTGGACCGGC ACCGCGAGGC CCAGCAGGTG 
CTGGCTCGGC TGGAGGAGGA AAACCAGCAG CTGCGGGGGT CCCTGGCTCC TGGTGGGGAG 
CCAGGCATCT CCTCAAGGGC CCCGGCATCC CCCCAAGTGG CTGCTCTGGA GCAAGACCTA 
GGGAAGCTGG AGGAGGAGCT GCGGGTGGTT CAGGCCACGA TGAGCGGGAA GAGCCAGGAG 
ATCGGGAAGC TGAAGCAGCT GCTCTACCAA GCCACAGAGG AGGTGGCAGA GCTGAGGGCC 
CGGGAGGCTG CCAGCCTACG GCAACATGAG AAAACTCGGG GTTCGCTGGT GGCCCAGGCT 
CAGGCTTGGG GTCAAGAGCT AAAGGCCCTG CTGGAAAAAT ATAACACAGC CTGCCAGGAG 
ATGGGTCGGC TGCGGGAGGC CATGGCAGAG GAGCGCCGCA GGAACGGGGA GCTGGCTGCT 
CAGGCCGCTG AGCAGGAGCA GCAGGCCAGA GAGATGCGCG GGCGGTCTGA GCAGTTTGAG 
AAAACAGCGG AGCTGCTGAA AGAGAAGATG GAGCATCTCA TTGGGGCTTG CCGGGACAAG 
GAGGCCAAGA TCAAGGAGCT GTTGAAGAAG CTGGAGCAGC TTTCAGAGGA GGTTCTAGCA 
GTTCGGGGAG AAAATGCTCG CCTTGTCCTG CAGCTGCAGG ATTACCAGAA GAACCACGAA 
GAGATCATCT CTACTTATAG GAATCATCTG CTGAATGCTG CTCGGGGCTA CATGGAACAG 
GATGTGTATG ATATCCTACT GCGAATCCTC AGCATACAAG AGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD35 ( XM_012761932.2 ) cDNA ORF clone, Microcebus murinus(gray mouse lemur) -> XP_012617386.2 Microcebus murinus ankyrin repeat domain 35 (ANKRD35), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMi73073
Clone ID Related Accession (Same CDS sequence)	XM_012761932.2
Accession Version	XM_012761932.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-23
Organism	Microcebus murinus(gray mouse lemur)
Product	ankyrin repeat domain-containing protein 35 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033661.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 24, 2017 this sequence version replaced XM_012761932.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microcebus murinus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACAGT TGAGAAAAGC GAAGCCCACC CGGGAAGCCG GGACCGTCGG CCTCCAGCCG 
AGGCGCGCCG AGGGGCGGGC GGAGGCGGCG TTTGGGCGGA TGCCGCCCGG GAGCTGCTGC 
TGGCGGGCGC AGTCGCGGGA GGAGTCTCGG CCGGAGCCGG CCCGGGGCCG CTCGGCAGCG 
AGCAGCCGCC TCCGAGTTCG GTGGGTTTGG GAACCTGTGG CCCGGCTCTC TGCGCGTCCC 
CGTCCCCCCG TGGCCCTGAC CCCGGCCATG AAGCGCATCT TCTCCTGCTC CAGCTCACAG 
GTGGCGGTGG AGAGATGGAA TCGTCGTGAC CAGAAGCTGC TGGAGGCAGT GCAGCGGGGG 
GACGTGGGAC GCGTGGCTGC CCTTGCCTCC AGGAAGTCGG CCCGGCCCAC CAAGCTAGAC 
TCCAATGGCC AGTCCCCGTT TCATCTGGCA GCCTCCAAAG GCCTGACGGA GTGTCTGACT 
ATACTGCTTG CAAATGGGGC TGACATCAAC AGCAAGAATG AGGATGGAAG TACTGCCTTG 
CACTTGGCCA CCATCTCCTG CCAGCCACAG TGTGTCAAGG TTCTGCTTCA GCATGGTGCT 
AATGAAGATG CTGTGGATGC AGAAAACCGT AGTCCATTGC ACTGGGCAGC CTCCTCTGGC 
TGTGCCTCAA GTGTGCTCCT GCTTTGTGAC CACGAAGCCT TCCTGGACGT GTTGGATAAT 
GATGGACGCA CACCCCTGAT GATCGCGTCG CTGGGCGGTC ACGCGGCTAT CTGCTCACAG 
TTGCTGCAGA GAGGCGCCCG AGTCAACGTT ACAGACAAGG ATGACAAGTC GGCTTTGATC 
CTGGCCTGTG AGAAAGGCAG TGCTGAGGTG GCAGAACTGC TCCTAGGCCA CGGAGCAGAC 
GCAGGGGCAA TGGACAGAAC AGGGCACGAT GCTCTCCATT ATGCTTTGCA CACGCAGGAC 
AAGGCACTGT GGAGGCTACT ACGGCAGGCC TTGAACCGGC GGCGGAGGGG CGGCGGTCAA 
AGGCTAGTCC AATACCCTGA TCTTGCATCT CAGGTCTCTC CATCTGAGCC CCAGATGGGT 
TCTCCACCTA AGAGCCCATG GAGAGCGGAG CCTGAGGAGG AACAGGAGGA AGAGGATGAT 
GAAGATCCAT GCTCAGAGGA GTGGAAGTGG AAGTATGAAG AGGAGCAGAG GAAAGTTTCT 
CAGTTGGAGC AGGAGCTGGT GTGCAAAACA GAGGAGTGTA AGACTCAAGC TGCAGCCTAC 
CTGGGCCTAG AGAACCAGAT TCGAGAGCAG GTGAAGGAGC TGGGGCTCCT CCTATCCCAA 
GAGCCTGCAG CTTCAGGAAG GCGAGGCTCT AGTCTCAGGC TTGGAGGGGA TGGCATGGAG 
CAGGGTTGTC CCCTGGACCT GCTGGTGGAG CGTATACAAG AGCTGAAGAA GCAGCAGCAG 
GTAGCAGCCA CAGTCAACCC AACATTAGCT CCCAGGAAAG CTGAGGATTC AGCTCCAGGA 
GAGGTTCAAT ATGAAGTCCA TGGAAGGTTC CAGCCACAAG AACAGGGGCC ACTCCAGAGC 
CCAAGGTCTG TGACCATTGG GAAAACCACA GAACAGCAAC TGACCACCAA TGGGGGACAT 
ACCCTTGGCC TTGATCATCC TAACCAGTTG CCTGCTGGCC AGAAGGAGAG GCCCCAGGCC 
CCAGGGGTGG AACCAGCAGG CACAGTGGTT GAACAAGTGG GCACAGCAGC CATGAACCAG 
CTTCTGCTAC AACTAAGGGA GGAGCTGGCT GCAGTTTGGC GAGAAAAGGA TGCTGCCCGG 
GGAGCTTTGT CAAGACCAGT CCTAGAGGGA GCCCTGGGGA CTCCCCGGGC TGAGGCTGCA 
GCAGCTGCCT GGGAGAAGAT GGAGGCCCGG TTGGAGCGGG TGCTCGTGAG GCTGGATCGG 
GCAAAGGCAG GACTACAGGT GAAACCTGCC CAGGAGTCCA GAGAGGGAGC CCCAAAGGCA 
GCCCCAGGGA GCATCAGAGA GAATGAAGAG GAGAAGAGGT CTCCTGGGGC TCGAGGAGAA 
CCTCTAGGGG CCCCTGGAGG AGAACAGGCC CCAGGAGGAG GCCTGCCAAA GGGACAGCTG 
GAGAAGGAAG TGTCAGCACT GAGACTGAGC AACAGTAACT TGCTGGAGGA GTTGGGGGAG 
CTGGGCCGGG AGCGCCAGAG GTTGCAGAGG GAGCTGCAGT CCCTGAGCCA GCGGCTGCAA 
CGCGAGTTTG TGCCCAAGCC AGAGGCGCAG GTCCAGCTGC AGCAGTTGCG GCAGAGCGTG 
GGGCTACTGA CAGATGAACT GGCCATGGAG AAAGAGGCCA CAGAGAAGCT GCGGAAGCGC 
CTGGCCTCCC AGAGCAGTGG CCTTCGAGGG CTGTGGGACT GCCTGCCCCC AGATCTAGTG 
GGCAAGGGGA GCACACGGAG CACGCTGGCC GAGCCCCTGG AGGAGCTGCA GGGCTGCATC 
AGCACCCTGG TGGACCGGCA CCGCGAGGCC CAGCAGGTGC TGGCTCGGCT GGAGGAGGAA 
AACCAGCAGC TGCGGGGGTC CCTGGCTCCT GGTGGGGAGC CAGGCATCTC CTCAAGGGCC 
CCGGCATCCC CCCAAGTGGC TGCTCTGGAG CAAGACCTAG GGAAGCTGGA GGAGGAGCTG 
CGGGTGGTTC AGGCCACGAT GAGCGGGAAG AGCCAGGAGA TCGGGAAGCT GAAGCAGCTG 
CTCTACCAAG CCACAGAGGA GGTGGCAGAG CTGAGGGCCC GGGAGGCTGC CAGCCTACGG 
CAACATGAGA AAACTCGGGG TTCGCTGGTG GCCCAGGCTC AGGCTTGGGG TCAAGAGCTA 
AAGGCCCTGC TGGAAAAATA TAACACAGCC TGCCAGGAGA TGGGTCGGCT GCGGGAGGCC 
ATGGCAGAGG AGCGCCGCAG GAACGGGGAG CTGGCTGCTC AGGCCGCTGA GCAGGAGCAG 
CAGGCCAGAG AGATGCGCGG GCGGTCTGAG CAGTTTGAGA AAACAGCGGA GCTGCTGAAA 
GAGAAGATGG AGCATCTCAT TGGGGCTTGC CGGGACAAGG AGGCCAAGAT CAAGGAGCTG 
TTGAAGAAGC TGGAGCAGCT TTCAGAGGAG GTTCTAGCAG TTCGGGGAGA AAATGCTCGC 
CTTGTCCTGC AGCTGCAGGA TTACCAGAAG AACCACGAAG AGATCATCTC TACTTATAGG 
AATCATCTGC TGAATGCTGC TCGGGGCTAC ATGGAACAGG ATGTGTATGA TATCCTACTG 
CGAATCCTCA GCATACAAGA GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012617386.2
CDS	16..3282
Translation	MRQLRKAKPTREAGTVGLQPRRAEGRAEAAFGRMPPGSCCWRAQSREESRPEPARGRSAASSRLRVRWVWEPVARLSARPRPPVALTPAMKRIFSCSSSQVAVERWNRRDQKLLEAVQRGDVGRVAALASRKSARPTKLDSNGQSPFHLAASKGLTECLTILLANGADINSKNEDGSTALHLATISCQPQCVKVLLQHGANEDAVDAENRSPLHWAASSGCASSVLLLCDHEAFLDVLDNDGRTPLMIASLGGHAAICSQLLQRGARVNVTDKDDKSALILACEKGSAEVAELLLGHGADAGAMDRTGHDALHYALHTQDKALWRLLRQALNRRRRGGGQRLVQYPDLASQVSPSEPQMGSPPKSPWRAEPEEEQEEEDDEDPCSEEWKWKYEEEQRKVSQLEQELVCKTEECKTQAAAYLGLENQIREQVKELGLLLSQEPAASGRRGSSLRLGGDGMEQGCPLDLLVERIQELKKQQQVAATVNPTLAPRKAEDSAPGEVQYEVHGRFQPQEQGPLQSPRSVTIGKTTEQQLTTNGGHTLGLDHPNQLPAGQKERPQAPGVEPAGTVVEQVGTAAMNQLLLQLREELAAVWREKDAARGALSRPVLEGALGTPRAEAAAAAWEKMEARLERVLVRLDRAKAGLQVKPAQESREGAPKAAPGSIRENEEEKRSPGARGEPLGAPGGEQAPGGGLPKGQLEKEVSALRLSNSNLLEELGELGRERQRLQRELQSLSQRLQREFVPKPEAQVQLQQLRQSVGLLTDELAMEKEATEKLRKRLASQSSGLRGLWDCLPPDLVGKGSTRSTLAEPLEELQGCISTLVDRHREAQQVLARLEEENQQLRGSLAPGGEPGISSRAPASPQVAALEQDLGKLEEELRVVQATMSGKSQEIGKLKQLLYQATEEVAELRAREAASLRQHEKTRGSLVAQAQAWGQELKALLEKYNTACQEMGRLREAMAEERRRNGELAAQAAEQEQQAREMRGRSEQFEKTAELLKEKMEHLIGACRDKEAKIKELLKKLEQLSEEVLAVRGENARLVLQLQDYQKNHEEIISTYRNHLLNAARGYMEQDVYDILLRILSIQEE

Target ORF information:

RefSeq Version	XM_012761932.2
Organism	Microcebus murinus(gray mouse lemur)
Definition	Microcebus murinus ankyrin repeat domain 35 (ANKRD35), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012761932.2

ORF Insert Sequence:

ATGCGACAGT TGAGAAAAGC GAAGCCCACC CGGGAAGCCG GGACCGTCGG CCTCCAGCCG 
AGGCGCGCCG AGGGGCGGGC GGAGGCGGCG TTTGGGCGGA TGCCGCCCGG GAGCTGCTGC 
TGGCGGGCGC AGTCGCGGGA GGAGTCTCGG CCGGAGCCGG CCCGGGGCCG CTCGGCAGCG 
AGCAGCCGCC TCCGAGTTCG GTGGGTTTGG GAACCTGTGG CCCGGCTCTC TGCGCGTCCC 
CGTCCCCCCG TGGCCCTGAC CCCGGCCATG AAGCGCATCT TCTCCTGCTC CAGCTCACAG 
GTGGCGGTGG AGAGATGGAA TCGTCGTGAC CAGAAGCTGC TGGAGGCAGT GCAGCGGGGG 
GACGTGGGAC GCGTGGCTGC CCTTGCCTCC AGGAAGTCGG CCCGGCCCAC CAAGCTAGAC 
TCCAATGGCC AGTCCCCGTT TCATCTGGCA GCCTCCAAAG GCCTGACGGA GTGTCTGACT 
ATACTGCTTG CAAATGGGGC TGACATCAAC AGCAAGAATG AGGATGGAAG TACTGCCTTG 
CACTTGGCCA CCATCTCCTG CCAGCCACAG TGTGTCAAGG TTCTGCTTCA GCATGGTGCT 
AATGAAGATG CTGTGGATGC AGAAAACCGT AGTCCATTGC ACTGGGCAGC CTCCTCTGGC 
TGTGCCTCAA GTGTGCTCCT GCTTTGTGAC CACGAAGCCT TCCTGGACGT GTTGGATAAT 
GATGGACGCA CACCCCTGAT GATCGCGTCG CTGGGCGGTC ACGCGGCTAT CTGCTCACAG 
TTGCTGCAGA GAGGCGCCCG AGTCAACGTT ACAGACAAGG ATGACAAGTC GGCTTTGATC 
CTGGCCTGTG AGAAAGGCAG TGCTGAGGTG GCAGAACTGC TCCTAGGCCA CGGAGCAGAC 
GCAGGGGCAA TGGACAGAAC AGGGCACGAT GCTCTCCATT ATGCTTTGCA CACGCAGGAC 
AAGGCACTGT GGAGGCTACT ACGGCAGGCC TTGAACCGGC GGCGGAGGGG CGGCGGTCAA 
AGGCTAGTCC AATACCCTGA TCTTGCATCT CAGGTCTCTC CATCTGAGCC CCAGATGGGT 
TCTCCACCTA AGAGCCCATG GAGAGCGGAG CCTGAGGAGG AACAGGAGGA AGAGGATGAT 
GAAGATCCAT GCTCAGAGGA GTGGAAGTGG AAGTATGAAG AGGAGCAGAG GAAAGTTTCT 
CAGTTGGAGC AGGAGCTGGT GTGCAAAACA GAGGAGTGTA AGACTCAAGC TGCAGCCTAC 
CTGGGCCTAG AGAACCAGAT TCGAGAGCAG GTGAAGGAGC TGGGGCTCCT CCTATCCCAA 
GAGCCTGCAG CTTCAGGAAG GCGAGGCTCT AGTCTCAGGC TTGGAGGGGA TGGCATGGAG 
CAGGGTTGTC CCCTGGACCT GCTGGTGGAG CGTATACAAG AGCTGAAGAA GCAGCAGCAG 
GTAGCAGCCA CAGTCAACCC AACATTAGCT CCCAGGAAAG CTGAGGATTC AGCTCCAGGA 
GAGGTTCAAT ATGAAGTCCA TGGAAGGTTC CAGCCACAAG AACAGGGGCC ACTCCAGAGC 
CCAAGGTCTG TGACCATTGG GAAAACCACA GAACAGCAAC TGACCACCAA TGGGGGACAT 
ACCCTTGGCC TTGATCATCC TAACCAGTTG CCTGCTGGCC AGAAGGAGAG GCCCCAGGCC 
CCAGGGGTGG AACCAGCAGG CACAGTGGTT GAACAAGTGG GCACAGCAGC CATGAACCAG 
CTTCTGCTAC AACTAAGGGA GGAGCTGGCT GCAGTTTGGC GAGAAAAGGA TGCTGCCCGG 
GGAGCTTTGT CAAGACCAGT CCTAGAGGGA GCCCTGGGGA CTCCCCGGGC TGAGGCTGCA 
GCAGCTGCCT GGGAGAAGAT GGAGGCCCGG TTGGAGCGGG TGCTCGTGAG GCTGGATCGG 
GCAAAGGCAG GACTACAGGT GAAACCTGCC CAGGAGTCCA GAGAGGGAGC CCCAAAGGCA 
GCCCCAGGGA GCATCAGAGA GAATGAAGAG GAGAAGAGGT CTCCTGGGGC TCGAGGAGAA 
CCTCTAGGGG CCCCTGGAGG AGAACAGGCC CCAGGAGGAG GCCTGCCAAA GGGACAGCTG 
GAGAAGGAAG TGTCAGCACT GAGACTGAGC AACAGTAACT TGCTGGAGGA GTTGGGGGAG 
CTGGGCCGGG AGCGCCAGAG GTTGCAGAGG GAGCTGCAGT CCCTGAGCCA GCGGCTGCAA 
CGCGAGTTTG TGCCCAAGCC AGAGGCGCAG GTCCAGCTGC AGCAGTTGCG GCAGAGCGTG 
GGGCTACTGA CAGATGAACT GGCCATGGAG AAAGAGGCCA CAGAGAAGCT GCGGAAGCGC 
CTGGCCTCCC AGAGCAGTGG CCTTCGAGGG CTGTGGGACT GCCTGCCCCC AGATCTAGTG 
GGCAAGGGGA GCACACGGAG CACGCTGGCC GAGCCCCTGG AGGAGCTGCA GGGCTGCATC 
AGCACCCTGG TGGACCGGCA CCGCGAGGCC CAGCAGGTGC TGGCTCGGCT GGAGGAGGAA 
AACCAGCAGC TGCGGGGGTC CCTGGCTCCT GGTGGGGAGC CAGGCATCTC CTCAAGGGCC 
CCGGCATCCC CCCAAGTGGC TGCTCTGGAG CAAGACCTAG GGAAGCTGGA GGAGGAGCTG 
CGGGTGGTTC AGGCCACGAT GAGCGGGAAG AGCCAGGAGA TCGGGAAGCT GAAGCAGCTG 
CTCTACCAAG CCACAGAGGA GGTGGCAGAG CTGAGGGCCC GGGAGGCTGC CAGCCTACGG 
CAACATGAGA AAACTCGGGG TTCGCTGGTG GCCCAGGCTC AGGCTTGGGG TCAAGAGCTA 
AAGGCCCTGC TGGAAAAATA TAACACAGCC TGCCAGGAGA TGGGTCGGCT GCGGGAGGCC 
ATGGCAGAGG AGCGCCGCAG GAACGGGGAG CTGGCTGCTC AGGCCGCTGA GCAGGAGCAG 
CAGGCCAGAG AGATGCGCGG GCGGTCTGAG CAGTTTGAGA AAACAGCGGA GCTGCTGAAA 
GAGAAGATGG AGCATCTCAT TGGGGCTTGC CGGGACAAGG AGGCCAAGAT CAAGGAGCTG 
TTGAAGAAGC TGGAGCAGCT TTCAGAGGAG GTTCTAGCAG TTCGGGGAGA AAATGCTCGC 
CTTGTCCTGC AGCTGCAGGA TTACCAGAAG AACCACGAAG AGATCATCTC TACTTATAGG 
AATCATCTGC TGAATGCTGC TCGGGGCTAC ATGGAACAGG ATGTGTATGA TATCCTACTG 
CGAATCCTCA GCATACAAGA GGAGTGA