LOC105860230 cDNA ORF clone, Microcebus murinus(gray mouse lemur)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105860230
Entrez Gene ID	105860230
Full Name	E3 ubiquitin-protein ligase Topors-like
Gene Type	protein-coding
Organism	Microcebus murinus(gray mouse lemur)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012744789.2	XP_012600243.2	LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase Topors-like

Pathways from BioSystems
Gene Ontology

The following LOC105860230 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105860230 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMi93725	XM_012744789.2 Latest version!	Microcebus murinus E3 ubiquitin-protein ligase Topors-like (LOC105860230), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105860230 ( XM_012744789.2 ) cDNA ORF clone, Microcebus murinus(gray mouse lemur) -> XP_012600243.2 Microcebus murinus E3 ubiquitin-protein ligase Topors-like (LOC105860230), mRNA.

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CloneID	OMi93725
Clone ID Related Accession (Same CDS sequence)	XM_012744789.2
Accession Version	XM_012744789.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2790bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-23
Organism	Microcebus murinus(gray mouse lemur)
Product	LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase Topors-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033667.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Feb 24, 2017 this sequence version replaced XM_012744789.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microcebus murinus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 2 indels internal stop codons :: corrected 2 genomic stop codons ##RefSeq-Attributes-END##

ATGGACCGCT GTTCACCGGG AGCTGGCACT GGCGCACCCC AGCAGACCGT AGCAGCTGAT 
GCATCTCCTG AGCCTAGGTG TCCTGTATGC TTGGATAGAT TCCATAATGT ATCTTACCCA 
AATTGCTGCT TACATAAGTT CTGTTTTCGC TGCATACGGG AGTGGTCCAA ACAGAAAGCC 
GAGTGTCCAC TATGTAAGCA GCCCTTTGGT TCCATCTTCC ATTCTGTGAG GGCAGACGAT 
AACTTCAAGG AGTATGTCCT AAGGGCTTCC TATGATGGTT CTGCTGTTCG ACGACGTCGC 
TCCCGTACAG CTGTGACAAG GGAACGAAAT GTGTCTGTAC CTAGTGGTAC CACGAACAGA 
AGAATGACTC CACCGGATAC TGGAGTTCTA TTTGAAGTGT TAGGCACTTC AACAAGAGGT 
AGGGATGTCG CAATTCCTGA GTTACTGAGT GAGTTTGTAA CAGCGAGGCC AACTACTGCC 
CATGAAATAT CTTTGATGAC AGTTCAAGAG CAAGATGCTA TTAATTTTAG ACGAGCTCTC 
TATCGTGCTG GTGCTCGAGT TAGAAGTGTC GAAGATGGTG GCCGCTCTAG GGATATTTCA 
GCTGAGTTTT TCTATAGAAA TCCAGCTTCC CTGCGCAGAA TAGTCCCCTG GGTAAGGCGG 
GAACTTATGG TCCTTTTAGG AGCTCGTGAA CCTTCAGTAG ATATCGTCCA GCTCATCATC 
ATGACCAACA TTACTCGCTA TGATTTGGAG AGCCAGGCAT TTGTGTCTGA TTTAAGACCT 
TTTTTTCTAG ATCTAACTGA GCATTTTGTG CATGAATTTA TCAGTTTTGC TCGATCTCCT 
TTTAACATGG CAGAGTTTGA CCAACACGCT AATTATGATT ACCCTGCACC TTCCAATGAA 
GAAAGCAGCC ATTCTGACTC TTCGGTCATA ACGATATCTC CAGATGAGTC TGAGACCCAA 
GAGCCAGATA TGGATGTGGC CACTGTTGGT CAGGCACCAT GGGATGATGA GACACCGGGA 
CCATCCTACA CAAGCTCAGA GCAGGCACAT GTTGCTGTGT CTTCCCTTTT AAATGATTCC 
GGAAGTTCAG GCGAAGAACT GGTCACAGGG GGAGCCATGT CTCATGTACA AGGAGTACAA 
ACCAACGAGG ACTTAAATAA TGACAGTGCT TCTTCTTCAA ATGATTGTGT CATTGTTGGG 
TTTATTAAAC CACTAGCTGA GAGGACCCCA GAAATTGTTC AGCTCTCTTC CGATTCTGAG 
GAGATAAGCA CTCGGGAAGA AAGGGAGACA GTGGAGACAC AAGAACAGGA CCAGCCTTTC 
AGTTCTGCTG ATAGTGATCT TAGTAGGTCT TCATCTCCGC TCTCTCTCCT TGGAAACAAT 
GAGCAAATGA ATGAAGGTCA TCCTGATTCA TCGGAGGAGG AAGAGGAACC ATCTACGTCG 
TCCTCGTGTA GAGACTTGAG CTCATCTGTG AGAGGAGACG GAGTATATTC TCCAGACAGC 
CATAGATACG GGAAAAGGGG AAGATCAAGA AGTTCAGATT CAAGTTCCCA GAATAGAAGT 
GAGCATGATA ACAATCCTAG AAAGCATCCT GGGAAGAGAA GAATGAAAAG CAAAAGGTCC 
AGAAGTAGGG AAAGTAGCCC TAGAAGGAGA AGAGACAAAA AGAGGTCCAG AACTAGAGAC 
AGCAGTTGGT CAAGCACCAG CCAAACTCTA TCTCTAAGTA GTGAAAGCAC AAGCAGATCA 
CAATCTCATA GTGATCACAG TAAAAGAAGG TCACGGAGCA GAAGTGGAGA TCGTTACTAT 
TTAAGAAATG ATTATGGAAG CAGATCTATG TGGGACAGGG ATGACTATGA ATCATCTTAC 
AGGAGGAGGC CTCTGTCCAG AGCTCATTAT CGCAGCCAAT CTTCAAGTCC AGAATCTGGA 
ATTCAGTCCT TCTCTCTAAG AAACAATGCC AGGGAGAAAA CTAATCCCAG TGAAGGACAA 
TATAACTATG ATGAAAGGCA CAGATCGAGG AGCCTGTCTG GTAACAGGTC AATGACTTCA 
TCTACAGGGC CTGAGCAGGG GAGAAATGAC AAACCTGGAG GGAAACGAAA ATACAAAGCA 
CGGCATTTGG AGGGTACTAA TGAAGTGGCT CAAGCATCTC GTCAATGTCC TTCTGCAGTA 
AAGGAGACTG ATTACCAAAA ATTTTCCTCA GAATTGGATG AAAACGACAA AAATGAGAGT 
GACAGCTTTT CAGACAGCCT ATCATCAGAT GCAGAGACAA AACGCAAGAG GAGAAAAAGG 
GCCCGGAGCC CGAGTGTAGA GGTAGTTTGC AAAGGAATAG CTACTGATAC AACCATACAT 
CACGAAAGGA AAAAGAAGAA ACACCATGGA GACAGTACTT CGCAGTCCCC AGTTGTAATT 
ACCATCGACA GTGAAAGCGA TAAGGATTCT GAAGTAAAAG CAGATGCAGA TTGGGACAAT 
AATGGGCCTC AAGACCCTCT AGAAGATGAG TTTTAGGCTC CTTCCTTGGA ACCATTTNGA 
ACTAAAGATG GAGTTAAAAG AGAAGATGAA TTTGGTGTCC TGGACAAGGA GCGTCATATT 
ACTGCACTTA CTAACAACTT GCATAATGCC AGAAAAACTG TGCATAGTAT CCCACCCCCA 
GCAACTTCAG TGGAACAAAC TCTTGATATA AGAAAAGAGA GCACATCTTC CTATGATTTG 
GAGACCAGCC CATCTACTAG AACTCAGTCA TCAAGGCAAT TGCCATCTCC ACAGACATGA 
TTAATGTCAG TGTCTCTTAG TAGAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012600243.2
CDS	422..3211
Translation	MDRCSPGAGTGAPQQTVAADASPEPRCPVCLDRFHNVSYPNCCLHKFCFRCIREWSKQKAECPLCKQPFGSIFHSVRADDNFKEYVLRASYDGSAVRRRRSRTAVTRERNVSVPSGTTNRRMTPPDTGVLFEVLGTSTRGRDVAIPELLSEFVTARPTTAHEISLMTVQEQDAINFRRALYRAGARVRSVEDGGRSRDISAEFFYRNPASLRRIVPWVRRELMVLLGAREPSVDIVQLIIMTNITRYDLESQAFVSDLRPFFLDLTEHFVHEFISFARSPFNMAEFDQHANYDYPAPSNEESSHSDSSVITISPDESETQEPDMDVATVGQAPWDDETPGPSYTSSEQAHVAVSSLLNDSGSSGEELVTGGAMSHVQGVQTNEDLNNDSASSSNDCVIVGFIKPLAERTPEIVQLSSDSEEISTREERETVETQEQDQPFSSADSDLSRSSSPLSLLGNNEQMNEGHPDSSEEEEEPSTSSSCRDLSSSVRGDGVYSPDSHRYGKRGRSRSSDSSSQNRSEHDNNPRKHPGKRRMKSKRSRSRESSPRRRRDKKRSRTRDSSWSSTSQTLSLSSESTSRSQSHSDHSKRRSRSRSGDRYYLRNDYGSRSMWDRDDYESSYRRRPLSRAHYRSQSSSPESGIQSFSLRNNAREKTNPSEGQYNYDERHRSRSLSGNRSMTSSTGPEQGRNDKPGGKRKYKARHLEGTNEVAQASRQCPSAVKETDYQKFSSELDENDKNESDSFSDSLSSDAETKRKRRKRARSPSVEVVCKGIATDTTIHHERKKKKHHGDSTSQSPVVITIDSESDKDSEVKADADWDNNGPQDPLEDEFAPSLEPFnullTKDGVKREDEFGVLDKERHITALTNNLHNARKTVHSIPPPATSVEQTLDIRKESTSSYDLETSPSTRTQSSRQLPSPQTLMSVSLSRD

Target ORF information:

RefSeq Version	XM_012744789.2
Organism	Microcebus murinus(gray mouse lemur)
Definition	Microcebus murinus E3 ubiquitin-protein ligase Topors-like (LOC105860230), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012744789.2

ORF Insert Sequence:

ATGGACCGCT GTTCACCGGG AGCTGGCACT GGCGCACCCC AGCAGACCGT AGCAGCTGAT 
GCATCTCCTG AGCCTAGGTG TCCTGTATGC TTGGATAGAT TCCATAATGT ATCTTACCCA 
AATTGCTGCT TACATAAGTT CTGTTTTCGC TGCATACGGG AGTGGTCCAA ACAGAAAGCC 
GAGTGTCCAC TATGTAAGCA GCCCTTTGGT TCCATCTTCC ATTCTGTGAG GGCAGACGAT 
AACTTCAAGG AGTATGTCCT AAGGGCTTCC TATGATGGTT CTGCTGTTCG ACGACGTCGC 
TCCCGTACAG CTGTGACAAG GGAACGAAAT GTGTCTGTAC CTAGTGGTAC CACGAACAGA 
AGAATGACTC CACCGGATAC TGGAGTTCTA TTTGAAGTGT TAGGCACTTC AACAAGAGGT 
AGGGATGTCG CAATTCCTGA GTTACTGAGT GAGTTTGTAA CAGCGAGGCC AACTACTGCC 
CATGAAATAT CTTTGATGAC AGTTCAAGAG CAAGATGCTA TTAATTTTAG ACGAGCTCTC 
TATCGTGCTG GTGCTCGAGT TAGAAGTGTC GAAGATGGTG GCCGCTCTAG GGATATTTCA 
GCTGAGTTTT TCTATAGAAA TCCAGCTTCC CTGCGCAGAA TAGTCCCCTG GGTAAGGCGG 
GAACTTATGG TCCTTTTAGG AGCTCGTGAA CCTTCAGTAG ATATCGTCCA GCTCATCATC 
ATGACCAACA TTACTCGCTA TGATTTGGAG AGCCAGGCAT TTGTGTCTGA TTTAAGACCT 
TTTTTTCTAG ATCTAACTGA GCATTTTGTG CATGAATTTA TCAGTTTTGC TCGATCTCCT 
TTTAACATGG CAGAGTTTGA CCAACACGCT AATTATGATT ACCCTGCACC TTCCAATGAA 
GAAAGCAGCC ATTCTGACTC TTCGGTCATA ACGATATCTC CAGATGAGTC TGAGACCCAA 
GAGCCAGATA TGGATGTGGC CACTGTTGGT CAGGCACCAT GGGATGATGA GACACCGGGA 
CCATCCTACA CAAGCTCAGA GCAGGCACAT GTTGCTGTGT CTTCCCTTTT AAATGATTCC 
GGAAGTTCAG GCGAAGAACT GGTCACAGGG GGAGCCATGT CTCATGTACA AGGAGTACAA 
ACCAACGAGG ACTTAAATAA TGACAGTGCT TCTTCTTCAA ATGATTGTGT CATTGTTGGG 
TTTATTAAAC CACTAGCTGA GAGGACCCCA GAAATTGTTC AGCTCTCTTC CGATTCTGAG 
GAGATAAGCA CTCGGGAAGA AAGGGAGACA GTGGAGACAC AAGAACAGGA CCAGCCTTTC 
AGTTCTGCTG ATAGTGATCT TAGTAGGTCT TCATCTCCGC TCTCTCTCCT TGGAAACAAT 
GAGCAAATGA ATGAAGGTCA TCCTGATTCA TCGGAGGAGG AAGAGGAACC ATCTACGTCG 
TCCTCGTGTA GAGACTTGAG CTCATCTGTG AGAGGAGACG GAGTATATTC TCCAGACAGC 
CATAGATACG GGAAAAGGGG AAGATCAAGA AGTTCAGATT CAAGTTCCCA GAATAGAAGT 
GAGCATGATA ACAATCCTAG AAAGCATCCT GGGAAGAGAA GAATGAAAAG CAAAAGGTCC 
AGAAGTAGGG AAAGTAGCCC TAGAAGGAGA AGAGACAAAA AGAGGTCCAG AACTAGAGAC 
AGCAGTTGGT CAAGCACCAG CCAAACTCTA TCTCTAAGTA GTGAAAGCAC AAGCAGATCA 
CAATCTCATA GTGATCACAG TAAAAGAAGG TCACGGAGCA GAAGTGGAGA TCGTTACTAT 
TTAAGAAATG ATTATGGAAG CAGATCTATG TGGGACAGGG ATGACTATGA ATCATCTTAC 
AGGAGGAGGC CTCTGTCCAG AGCTCATTAT CGCAGCCAAT CTTCAAGTCC AGAATCTGGA 
ATTCAGTCCT TCTCTCTAAG AAACAATGCC AGGGAGAAAA CTAATCCCAG TGAAGGACAA 
TATAACTATG ATGAAAGGCA CAGATCGAGG AGCCTGTCTG GTAACAGGTC AATGACTTCA 
TCTACAGGGC CTGAGCAGGG GAGAAATGAC AAACCTGGAG GGAAACGAAA ATACAAAGCA 
CGGCATTTGG AGGGTACTAA TGAAGTGGCT CAAGCATCTC GTCAATGTCC TTCTGCAGTA 
AAGGAGACTG ATTACCAAAA ATTTTCCTCA GAATTGGATG AAAACGACAA AAATGAGAGT 
GACAGCTTTT CAGACAGCCT ATCATCAGAT GCAGAGACAA AACGCAAGAG GAGAAAAAGG 
GCCCGGAGCC CGAGTGTAGA GGTAGTTTGC AAAGGAATAG CTACTGATAC AACCATACAT 
CACGAAAGGA AAAAGAAGAA ACACCATGGA GACAGTACTT CGCAGTCCCC AGTTGTAATT 
ACCATCGACA GTGAAAGCGA TAAGGATTCT GAAGTAAAAG CAGATGCAGA TTGGGACAAT 
AATGGGCCTC AAGACCCTCT AGAAGATGAG TTTTAGGCTC CTTCCTTGGA ACCATTTNGA 
ACTAAAGATG GAGTTAAAAG AGAAGATGAA TTTGGTGTCC TGGACAAGGA GCGTCATATT 
ACTGCACTTA CTAACAACTT GCATAATGCC AGAAAAACTG TGCATAGTAT CCCACCCCCA 
GCAACTTCAG TGGAACAAAC TCTTGATATA AGAAAAGAGA GCACATCTTC CTATGATTTG 
GAGACCAGCC CATCTACTAG AACTCAGTCA TCAAGGCAAT TGCCATCTCC ACAGACATGA 
TTAATGTCAG TGTCTCTTAG TAGAGACTGA