LOC105663106 gene cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

mRNA	Protein	Name
XM_012290272.1	XP_012145662.1	uncharacterized protein LOC105663106 isoform X1
XM_012290273.1	XP_012145663.1	uncharacterized protein LOC105663106 isoform X1
XM_012290274.1	XP_012145664.1	uncharacterized protein LOC105663106 isoform X1
XM_012290275.1	XP_012145665.1	uncharacterized protein LOC105663106 isoform X1
XM_012290276.1	XP_012145666.1	uncharacterized protein LOC105663106 isoform X1
XM_012290278.1	XP_012145668.1	uncharacterized protein LOC105663106 isoform X1
XM_012290283.1	XP_012145673.1	uncharacterized protein LOC105663106 isoform X4
XM_012290284.1	XP_012145674.1	uncharacterized protein LOC105663106 isoform X4
XM_012290282.1	XP_012145672.1	uncharacterized protein LOC105663106 isoform X4
XM_012290285.1	XP_012145675.1	uncharacterized protein LOC105663106 isoform X4
XM_012290279.1	XP_012145669.1	uncharacterized protein LOC105663106 isoform X1
XM_012290277.1	XP_012145667.1	uncharacterized protein LOC105663106 isoform X1
XM_012290280.1	XP_012145670.1	uncharacterized protein LOC105663106 isoform X2
XM_012290281.1	XP_012145671.1	uncharacterized protein LOC105663106 isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMe108972	XM_012290278.1 Latest version!	Megachile rotundata uncharacterized LOC105663106 (LOC105663106), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OMe108972	XM_012290272.1 Latest version!	Megachile rotundata uncharacterized LOC105663106 (LOC105663106), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OMe108972	XM_012290275.1 Latest version!	Megachile rotundata uncharacterized LOC105663106 (LOC105663106), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OMe108972	XM_012290279.1 Latest version!	Megachile rotundata uncharacterized LOC105663106 (LOC105663106), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OMe108972	XM_012290276.1 Latest version!	Megachile rotundata uncharacterized LOC105663106 (LOC105663106), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OMe108972	XM_012290273.1 Latest version!	Megachile rotundata uncharacterized LOC105663106 (LOC105663106), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OMe108972	XM_012290277.1 Latest version!	Megachile rotundata uncharacterized LOC105663106 (LOC105663106), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OMe108972	XM_012290274.1 Latest version!	Megachile rotundata uncharacterized LOC105663106 (LOC105663106), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OMe108973	XM_012290280.1 Latest version!	Megachile rotundata uncharacterized LOC105663106 (LOC105663106), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OMe108974	XM_012290281.1 Latest version!	Megachile rotundata uncharacterized LOC105663106 (LOC105663106), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OMe108975	XM_012290285.1 Latest version!	Megachile rotundata uncharacterized LOC105663106 (LOC105663106), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OMe108975	XM_012290284.1 Latest version!	Megachile rotundata uncharacterized LOC105663106 (LOC105663106), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OMe108975	XM_012290283.1 Latest version!	Megachile rotundata uncharacterized LOC105663106 (LOC105663106), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OMe108975	XM_012290282.1 Latest version!	Megachile rotundata uncharacterized LOC105663106 (LOC105663106), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

LOC105663106 ( XM_012290272.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012145662.1 Megachile rotundata uncharacterized LOC105663106 (LOC105663106), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMe108972
Clone ID Related Accession (Same CDS sequence)	XM_012290272.1 , XM_012290273.1 , XM_012290274.1 , XM_012290275.1 , XM_012290276.1 , XM_012290278.1 , XM_012290279.1 , XM_012290277.1
Accession Version	XM_012290272.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	uncharacterized protein LOC105663106 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797277.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACGG AAGCGAAGGA GCGAAGCAGA GCACAGGCAA AGAATTTGCT TTTACGTATG 
AAGCAAAAAA AAAAGGCTTA TTATTCAAAA CGTTTGTCCA AAAGAAGATT ATTGAGAAAC 
TTACGTAAGT TAAGAAACCA AAAAGCGGCC TCTATGTCAA ACGACGATCA AAATAATGAA 
GCAAATCAAC AAACTGACCA AACTAAAGTT GGCGAAATAA AAGCAGATGG TGAACACAAC 
GAAATAAAAA CAAATGCCGA ATGCAACGAA ATAAAAACAG ATGCTGAATG CAACGAAACA 
AAAGCAGATG ATTGTAGAAT TATCTGCTAC CAAAGTCTTC CACTCAATAA TAAAGGCTCT 
CGCAAGTGCA GTCTAAACTG TTTTCCTCTA TGTAAAATAT ATGAAGACTA TAAAGGAAAG 
CTCGAGGAGC TTAAAATTAT TAAAGGAAGT ATGGAACTAA TCGACTTCAA TGAAGAGGAA 
CAACGTGAAA TGAGCAGCAT CAACGGAACT ATGCAAGTAT ACGCTATAAT ACCGAAAGAG 
AATGAAAAAG ATATATTGGA TGAAACGCAA GAAACATCAA ATCTAAATAA TGATTACGAG 
GCTTTAAATC AAGAAATTAT GTCTTTAAGC CAAGAGACTA TACCTTCGAG TCAGGAAACT 
ATTTCGGATG ATACTGACCT CGAATGCAAT TGGATAGTAG AAAAAGCAAA ACCGCAAACG 
CAGGAACAAC CAATTTTGGA AGGAATAGAT TTTGTCGATT TTAAATATGT TTTTAATCAA 
TTATTAACAC TCGACGATCA TTCCAACATC GGATGCAGCA TTCGACATTT TAAAATAATT 
GGACGTTACC GGAATGGACT GAAAATAACA TATTCTGTAA AATGTGAAAT GTGCAATCAG 
ATAATACATA TACCATGCAT TCAACAGAAT AATGACGTTA TAGATATCAA TGAGAGCGCT 
GTTTCCGCGA CAATGGTATT AGGCGGCGGA TATGTTCAAC TGATGGAGAT GTTGGCTTGT 
CTGAATGTGT CGTGTATGTC CAAACAATCA TACGACAAAC ACAGAACAAA AATAATAGAG 
GCCTTCGAAC TGGCTGCACA GTGGGAAATG GAAGCTGCTG GTAAAAAAGA GAGAGAACTA 
GCTATAGCAA GAGGTGATGT ATGTCCAGAC TCTGGCATAC CATGGATTCC AGTTGTCGCT 
GATGGTAGCT GGATGAAAAG ATCATACAAA GGTGGTTATT ATAATTCTCC TTCCGGTGTA 
GGAGTTATTG TTGGATATTA TACAAAGAAA GTGTTATATG TTGGCGTAAA GAATAAAGTC 
TGCATAACTT GCCGGGTAGC GGCTAAGAAA AAAGAAAAAC CTAGCGAGCA TCGTTGCTTC 
AAAAATTGGG GTACAACTCA GAGTTCAACT GCAATGGAAA GCGTTGCTAT TTTGGAGGGA 
TTTGAATGCA GCATAAAAAT GCACGGCCTG ATATATTCAA TATTAATTGC CGATGGTGAT 
AGCAGTGTCT ACAAAAAGAT ATTGGACCGT GATCCGTATA AACAGTACTC GGTGCGTGTA 
CAAAAAATTG AATGTATCAA TCACATGCTA CGCAATTTCG GTAAGAAAAT TGCTAATATC 
GGCACTACAG GTGGGTGCCA AAAATTAAGA GCTGTCGTAA AAAAAAACGC TCTCCGTCTG 
CGCGCTTGTA TAAGGAAAGC TGCACAATAC CGCTATAACG AAAACCTGCC ACTGGTTGAA 
AAACTTGAAA GACTAAAAGC AGATTTAACT AACGTGCCTA GCCACGTTTT CGGAGAACAC 
AAGGACTGTG AAAGATTGGC ATATTTCTGC AACCAGTACT CTGCTCCCAA CGTAATAAAC 
TATGTTCCGC AACTGAAGGA AGCAGGTATT TATAAACGTA TAGAGGAAGC CATGCAGCCG 
CTTTTCAAGA ATATTCAAAG TTTGCTGTAC TGTATGAATA ATAATGCAGC AGAAGTCTTT 
AATGCCATCA TCGCGAAATA CATTGGTGGC AAACGAATAA ACTTTGGATT AAAAGGCTCG 
TATCAGGGTA GGGTTTACGC TGCTGTCGTT CAGTACAATA CAAAAGAGGC AGTGAGCCGA 
ATGTGTAGAG CAATAAATAA GGAACCGCCA GTCCTTGTCA GGAAGATTGA AGAACGACGT 
AAAAGCGAGA ATAGAAGATA TTATATATCT TCCAGGCGGC GTAAAAGACA AATGTGTGAT 
AAGGATTACG GTCCTTGTGC GGAACAACCT GAGATAGAAG AAATAGTTTT CACTCCCGAG 
TATGAGAAAA AAATAGCAGC ACTCCACAAA TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012145662.1
CDS	241..2556
Translation	MDTEAKERSRAQAKNLLLRMKQKKKAYYSKRLSKRRLLRNLRKLRNQKAASMSNDDQNNEANQQTDQTKVGEIKADGEHNEIKTNAECNEIKTDAECNETKADDCRIICYQSLPLNNKGSRKCSLNCFPLCKIYEDYKGKLEELKIIKGSMELIDFNEEEQREMSSINGTMQVYAIIPKENEKDILDETQETSNLNNDYEALNQEIMSLSQETIPSSQETISDDTDLECNWIVEKAKPQTQEQPILEGIDFVDFKYVFNQLLTLDDHSNIGCSIRHFKIIGRYRNGLKITYSVKCEMCNQIIHIPCIQQNNDVIDINESAVSATMVLGGGYVQLMEMLACLNVSCMSKQSYDKHRTKIIEAFELAAQWEMEAAGKKERELAIARGDVCPDSGIPWIPVVADGSWMKRSYKGGYYNSPSGVGVIVGYYTKKVLYVGVKNKVCITCRVAAKKKEKPSEHRCFKNWGTTQSSTAMESVAILEGFECSIKMHGLIYSILIADGDSSVYKKILDRDPYKQYSVRVQKIECINHMLRNFGKKIANIGTTGGCQKLRAVVKKNALRLRACIRKAAQYRYNENLPLVEKLERLKADLTNVPSHVFGEHKDCERLAYFCNQYSAPNVINYVPQLKEAGIYKRIEEAMQPLFKNIQSLLYCMNNNAAEVFNAIIAKYIGGKRINFGLKGSYQGRVYAAVVQYNTKEAVSRMCRAINKEPPVLVRKIEERRKSENRRYYISSRRRKRQMCDKDYGPCAEQPEIEEIVFTPEYEKKIAALHKC

Target ORF information:

RefSeq Version	XM_012290272.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata uncharacterized LOC105663106 (LOC105663106), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012290272.1

ORF Insert Sequence:

ATGGACACGG AAGCGAAGGA GCGAAGCAGA GCACAGGCAA AGAATTTGCT TTTACGTATG 
AAGCAAAAAA AAAAGGCTTA TTATTCAAAA CGTTTGTCCA AAAGAAGATT ATTGAGAAAC 
TTACGTAAGT TAAGAAACCA AAAAGCGGCC TCTATGTCAA ACGACGATCA AAATAATGAA 
GCAAATCAAC AAACTGACCA AACTAAAGTT GGCGAAATAA AAGCAGATGG TGAACACAAC 
GAAATAAAAA CAAATGCCGA ATGCAACGAA ATAAAAACAG ATGCTGAATG CAACGAAACA 
AAAGCAGATG ATTGTAGAAT TATCTGCTAC CAAAGTCTTC CACTCAATAA TAAAGGCTCT 
CGCAAGTGCA GTCTAAACTG TTTTCCTCTA TGTAAAATAT ATGAAGACTA TAAAGGAAAG 
CTCGAGGAGC TTAAAATTAT TAAAGGAAGT ATGGAACTAA TCGACTTCAA TGAAGAGGAA 
CAACGTGAAA TGAGCAGCAT CAACGGAACT ATGCAAGTAT ACGCTATAAT ACCGAAAGAG 
AATGAAAAAG ATATATTGGA TGAAACGCAA GAAACATCAA ATCTAAATAA TGATTACGAG 
GCTTTAAATC AAGAAATTAT GTCTTTAAGC CAAGAGACTA TACCTTCGAG TCAGGAAACT 
ATTTCGGATG ATACTGACCT CGAATGCAAT TGGATAGTAG AAAAAGCAAA ACCGCAAACG 
CAGGAACAAC CAATTTTGGA AGGAATAGAT TTTGTCGATT TTAAATATGT TTTTAATCAA 
TTATTAACAC TCGACGATCA TTCCAACATC GGATGCAGCA TTCGACATTT TAAAATAATT 
GGACGTTACC GGAATGGACT GAAAATAACA TATTCTGTAA AATGTGAAAT GTGCAATCAG 
ATAATACATA TACCATGCAT TCAACAGAAT AATGACGTTA TAGATATCAA TGAGAGCGCT 
GTTTCCGCGA CAATGGTATT AGGCGGCGGA TATGTTCAAC TGATGGAGAT GTTGGCTTGT 
CTGAATGTGT CGTGTATGTC CAAACAATCA TACGACAAAC ACAGAACAAA AATAATAGAG 
GCCTTCGAAC TGGCTGCACA GTGGGAAATG GAAGCTGCTG GTAAAAAAGA GAGAGAACTA 
GCTATAGCAA GAGGTGATGT ATGTCCAGAC TCTGGCATAC CATGGATTCC AGTTGTCGCT 
GATGGTAGCT GGATGAAAAG ATCATACAAA GGTGGTTATT ATAATTCTCC TTCCGGTGTA 
GGAGTTATTG TTGGATATTA TACAAAGAAA GTGTTATATG TTGGCGTAAA GAATAAAGTC 
TGCATAACTT GCCGGGTAGC GGCTAAGAAA AAAGAAAAAC CTAGCGAGCA TCGTTGCTTC 
AAAAATTGGG GTACAACTCA GAGTTCAACT GCAATGGAAA GCGTTGCTAT TTTGGAGGGA 
TTTGAATGCA GCATAAAAAT GCACGGCCTG ATATATTCAA TATTAATTGC CGATGGTGAT 
AGCAGTGTCT ACAAAAAGAT ATTGGACCGT GATCCGTATA AACAGTACTC GGTGCGTGTA 
CAAAAAATTG AATGTATCAA TCACATGCTA CGCAATTTCG GTAAGAAAAT TGCTAATATC 
GGCACTACAG GTGGGTGCCA AAAATTAAGA GCTGTCGTAA AAAAAAACGC TCTCCGTCTG 
CGCGCTTGTA TAAGGAAAGC TGCACAATAC CGCTATAACG AAAACCTGCC ACTGGTTGAA 
AAACTTGAAA GACTAAAAGC AGATTTAACT AACGTGCCTA GCCACGTTTT CGGAGAACAC 
AAGGACTGTG AAAGATTGGC ATATTTCTGC AACCAGTACT CTGCTCCCAA CGTAATAAAC 
TATGTTCCGC AACTGAAGGA AGCAGGTATT TATAAACGTA TAGAGGAAGC CATGCAGCCG 
CTTTTCAAGA ATATTCAAAG TTTGCTGTAC TGTATGAATA ATAATGCAGC AGAAGTCTTT 
AATGCCATCA TCGCGAAATA CATTGGTGGC AAACGAATAA ACTTTGGATT AAAAGGCTCG 
TATCAGGGTA GGGTTTACGC TGCTGTCGTT CAGTACAATA CAAAAGAGGC AGTGAGCCGA 
ATGTGTAGAG CAATAAATAA GGAACCGCCA GTCCTTGTCA GGAAGATTGA AGAACGACGT 
AAAAGCGAGA ATAGAAGATA TTATATATCT TCCAGGCGGC GTAAAAGACA AATGTGTGAT 
AAGGATTACG GTCCTTGTGC GGAACAACCT GAGATAGAAG AAATAGTTTT CACTCCCGAG 
TATGAGAAAA AAATAGCAGC ACTCCACAAA TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105663106 ( XM_012290273.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012145663.1 Megachile rotundata uncharacterized LOC105663106 (LOC105663106), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMe108972
Clone ID Related Accession (Same CDS sequence)	XM_012290272.1 , XM_012290273.1 , XM_012290274.1 , XM_012290275.1 , XM_012290276.1 , XM_012290278.1 , XM_012290279.1 , XM_012290277.1
Accession Version	XM_012290273.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	uncharacterized protein LOC105663106 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797277.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACGG AAGCGAAGGA GCGAAGCAGA GCACAGGCAA AGAATTTGCT TTTACGTATG 
AAGCAAAAAA AAAAGGCTTA TTATTCAAAA CGTTTGTCCA AAAGAAGATT ATTGAGAAAC 
TTACGTAAGT TAAGAAACCA AAAAGCGGCC TCTATGTCAA ACGACGATCA AAATAATGAA 
GCAAATCAAC AAACTGACCA AACTAAAGTT GGCGAAATAA AAGCAGATGG TGAACACAAC 
GAAATAAAAA CAAATGCCGA ATGCAACGAA ATAAAAACAG ATGCTGAATG CAACGAAACA 
AAAGCAGATG ATTGTAGAAT TATCTGCTAC CAAAGTCTTC CACTCAATAA TAAAGGCTCT 
CGCAAGTGCA GTCTAAACTG TTTTCCTCTA TGTAAAATAT ATGAAGACTA TAAAGGAAAG 
CTCGAGGAGC TTAAAATTAT TAAAGGAAGT ATGGAACTAA TCGACTTCAA TGAAGAGGAA 
CAACGTGAAA TGAGCAGCAT CAACGGAACT ATGCAAGTAT ACGCTATAAT ACCGAAAGAG 
AATGAAAAAG ATATATTGGA TGAAACGCAA GAAACATCAA ATCTAAATAA TGATTACGAG 
GCTTTAAATC AAGAAATTAT GTCTTTAAGC CAAGAGACTA TACCTTCGAG TCAGGAAACT 
ATTTCGGATG ATACTGACCT CGAATGCAAT TGGATAGTAG AAAAAGCAAA ACCGCAAACG 
CAGGAACAAC CAATTTTGGA AGGAATAGAT TTTGTCGATT TTAAATATGT TTTTAATCAA 
TTATTAACAC TCGACGATCA TTCCAACATC GGATGCAGCA TTCGACATTT TAAAATAATT 
GGACGTTACC GGAATGGACT GAAAATAACA TATTCTGTAA AATGTGAAAT GTGCAATCAG 
ATAATACATA TACCATGCAT TCAACAGAAT AATGACGTTA TAGATATCAA TGAGAGCGCT 
GTTTCCGCGA CAATGGTATT AGGCGGCGGA TATGTTCAAC TGATGGAGAT GTTGGCTTGT 
CTGAATGTGT CGTGTATGTC CAAACAATCA TACGACAAAC ACAGAACAAA AATAATAGAG 
GCCTTCGAAC TGGCTGCACA GTGGGAAATG GAAGCTGCTG GTAAAAAAGA GAGAGAACTA 
GCTATAGCAA GAGGTGATGT ATGTCCAGAC TCTGGCATAC CATGGATTCC AGTTGTCGCT 
GATGGTAGCT GGATGAAAAG ATCATACAAA GGTGGTTATT ATAATTCTCC TTCCGGTGTA 
GGAGTTATTG TTGGATATTA TACAAAGAAA GTGTTATATG TTGGCGTAAA GAATAAAGTC 
TGCATAACTT GCCGGGTAGC GGCTAAGAAA AAAGAAAAAC CTAGCGAGCA TCGTTGCTTC 
AAAAATTGGG GTACAACTCA GAGTTCAACT GCAATGGAAA GCGTTGCTAT TTTGGAGGGA 
TTTGAATGCA GCATAAAAAT GCACGGCCTG ATATATTCAA TATTAATTGC CGATGGTGAT 
AGCAGTGTCT ACAAAAAGAT ATTGGACCGT GATCCGTATA AACAGTACTC GGTGCGTGTA 
CAAAAAATTG AATGTATCAA TCACATGCTA CGCAATTTCG GTAAGAAAAT TGCTAATATC 
GGCACTACAG GTGGGTGCCA AAAATTAAGA GCTGTCGTAA AAAAAAACGC TCTCCGTCTG 
CGCGCTTGTA TAAGGAAAGC TGCACAATAC CGCTATAACG AAAACCTGCC ACTGGTTGAA 
AAACTTGAAA GACTAAAAGC AGATTTAACT AACGTGCCTA GCCACGTTTT CGGAGAACAC 
AAGGACTGTG AAAGATTGGC ATATTTCTGC AACCAGTACT CTGCTCCCAA CGTAATAAAC 
TATGTTCCGC AACTGAAGGA AGCAGGTATT TATAAACGTA TAGAGGAAGC CATGCAGCCG 
CTTTTCAAGA ATATTCAAAG TTTGCTGTAC TGTATGAATA ATAATGCAGC AGAAGTCTTT 
AATGCCATCA TCGCGAAATA CATTGGTGGC AAACGAATAA ACTTTGGATT AAAAGGCTCG 
TATCAGGGTA GGGTTTACGC TGCTGTCGTT CAGTACAATA CAAAAGAGGC AGTGAGCCGA 
ATGTGTAGAG CAATAAATAA GGAACCGCCA GTCCTTGTCA GGAAGATTGA AGAACGACGT 
AAAAGCGAGA ATAGAAGATA TTATATATCT TCCAGGCGGC GTAAAAGACA AATGTGTGAT 
AAGGATTACG GTCCTTGTGC GGAACAACCT GAGATAGAAG AAATAGTTTT CACTCCCGAG 
TATGAGAAAA AAATAGCAGC ACTCCACAAA TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012145663.1
CDS	241..2556
Translation	MDTEAKERSRAQAKNLLLRMKQKKKAYYSKRLSKRRLLRNLRKLRNQKAASMSNDDQNNEANQQTDQTKVGEIKADGEHNEIKTNAECNEIKTDAECNETKADDCRIICYQSLPLNNKGSRKCSLNCFPLCKIYEDYKGKLEELKIIKGSMELIDFNEEEQREMSSINGTMQVYAIIPKENEKDILDETQETSNLNNDYEALNQEIMSLSQETIPSSQETISDDTDLECNWIVEKAKPQTQEQPILEGIDFVDFKYVFNQLLTLDDHSNIGCSIRHFKIIGRYRNGLKITYSVKCEMCNQIIHIPCIQQNNDVIDINESAVSATMVLGGGYVQLMEMLACLNVSCMSKQSYDKHRTKIIEAFELAAQWEMEAAGKKERELAIARGDVCPDSGIPWIPVVADGSWMKRSYKGGYYNSPSGVGVIVGYYTKKVLYVGVKNKVCITCRVAAKKKEKPSEHRCFKNWGTTQSSTAMESVAILEGFECSIKMHGLIYSILIADGDSSVYKKILDRDPYKQYSVRVQKIECINHMLRNFGKKIANIGTTGGCQKLRAVVKKNALRLRACIRKAAQYRYNENLPLVEKLERLKADLTNVPSHVFGEHKDCERLAYFCNQYSAPNVINYVPQLKEAGIYKRIEEAMQPLFKNIQSLLYCMNNNAAEVFNAIIAKYIGGKRINFGLKGSYQGRVYAAVVQYNTKEAVSRMCRAINKEPPVLVRKIEERRKSENRRYYISSRRRKRQMCDKDYGPCAEQPEIEEIVFTPEYEKKIAALHKC

Target ORF information:

RefSeq Version	XM_012290273.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata uncharacterized LOC105663106 (LOC105663106), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012290273.1

ORF Insert Sequence:

ATGGACACGG AAGCGAAGGA GCGAAGCAGA GCACAGGCAA AGAATTTGCT TTTACGTATG 
AAGCAAAAAA AAAAGGCTTA TTATTCAAAA CGTTTGTCCA AAAGAAGATT ATTGAGAAAC 
TTACGTAAGT TAAGAAACCA AAAAGCGGCC TCTATGTCAA ACGACGATCA AAATAATGAA 
GCAAATCAAC AAACTGACCA AACTAAAGTT GGCGAAATAA AAGCAGATGG TGAACACAAC 
GAAATAAAAA CAAATGCCGA ATGCAACGAA ATAAAAACAG ATGCTGAATG CAACGAAACA 
AAAGCAGATG ATTGTAGAAT TATCTGCTAC CAAAGTCTTC CACTCAATAA TAAAGGCTCT 
CGCAAGTGCA GTCTAAACTG TTTTCCTCTA TGTAAAATAT ATGAAGACTA TAAAGGAAAG 
CTCGAGGAGC TTAAAATTAT TAAAGGAAGT ATGGAACTAA TCGACTTCAA TGAAGAGGAA 
CAACGTGAAA TGAGCAGCAT CAACGGAACT ATGCAAGTAT ACGCTATAAT ACCGAAAGAG 
AATGAAAAAG ATATATTGGA TGAAACGCAA GAAACATCAA ATCTAAATAA TGATTACGAG 
GCTTTAAATC AAGAAATTAT GTCTTTAAGC CAAGAGACTA TACCTTCGAG TCAGGAAACT 
ATTTCGGATG ATACTGACCT CGAATGCAAT TGGATAGTAG AAAAAGCAAA ACCGCAAACG 
CAGGAACAAC CAATTTTGGA AGGAATAGAT TTTGTCGATT TTAAATATGT TTTTAATCAA 
TTATTAACAC TCGACGATCA TTCCAACATC GGATGCAGCA TTCGACATTT TAAAATAATT 
GGACGTTACC GGAATGGACT GAAAATAACA TATTCTGTAA AATGTGAAAT GTGCAATCAG 
ATAATACATA TACCATGCAT TCAACAGAAT AATGACGTTA TAGATATCAA TGAGAGCGCT 
GTTTCCGCGA CAATGGTATT AGGCGGCGGA TATGTTCAAC TGATGGAGAT GTTGGCTTGT 
CTGAATGTGT CGTGTATGTC CAAACAATCA TACGACAAAC ACAGAACAAA AATAATAGAG 
GCCTTCGAAC TGGCTGCACA GTGGGAAATG GAAGCTGCTG GTAAAAAAGA GAGAGAACTA 
GCTATAGCAA GAGGTGATGT ATGTCCAGAC TCTGGCATAC CATGGATTCC AGTTGTCGCT 
GATGGTAGCT GGATGAAAAG ATCATACAAA GGTGGTTATT ATAATTCTCC TTCCGGTGTA 
GGAGTTATTG TTGGATATTA TACAAAGAAA GTGTTATATG TTGGCGTAAA GAATAAAGTC 
TGCATAACTT GCCGGGTAGC GGCTAAGAAA AAAGAAAAAC CTAGCGAGCA TCGTTGCTTC 
AAAAATTGGG GTACAACTCA GAGTTCAACT GCAATGGAAA GCGTTGCTAT TTTGGAGGGA 
TTTGAATGCA GCATAAAAAT GCACGGCCTG ATATATTCAA TATTAATTGC CGATGGTGAT 
AGCAGTGTCT ACAAAAAGAT ATTGGACCGT GATCCGTATA AACAGTACTC GGTGCGTGTA 
CAAAAAATTG AATGTATCAA TCACATGCTA CGCAATTTCG GTAAGAAAAT TGCTAATATC 
GGCACTACAG GTGGGTGCCA AAAATTAAGA GCTGTCGTAA AAAAAAACGC TCTCCGTCTG 
CGCGCTTGTA TAAGGAAAGC TGCACAATAC CGCTATAACG AAAACCTGCC ACTGGTTGAA 
AAACTTGAAA GACTAAAAGC AGATTTAACT AACGTGCCTA GCCACGTTTT CGGAGAACAC 
AAGGACTGTG AAAGATTGGC ATATTTCTGC AACCAGTACT CTGCTCCCAA CGTAATAAAC 
TATGTTCCGC AACTGAAGGA AGCAGGTATT TATAAACGTA TAGAGGAAGC CATGCAGCCG 
CTTTTCAAGA ATATTCAAAG TTTGCTGTAC TGTATGAATA ATAATGCAGC AGAAGTCTTT 
AATGCCATCA TCGCGAAATA CATTGGTGGC AAACGAATAA ACTTTGGATT AAAAGGCTCG 
TATCAGGGTA GGGTTTACGC TGCTGTCGTT CAGTACAATA CAAAAGAGGC AGTGAGCCGA 
ATGTGTAGAG CAATAAATAA GGAACCGCCA GTCCTTGTCA GGAAGATTGA AGAACGACGT 
AAAAGCGAGA ATAGAAGATA TTATATATCT TCCAGGCGGC GTAAAAGACA AATGTGTGAT 
AAGGATTACG GTCCTTGTGC GGAACAACCT GAGATAGAAG AAATAGTTTT CACTCCCGAG 
TATGAGAAAA AAATAGCAGC ACTCCACAAA TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105663106 ( XM_012290274.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012145664.1 Megachile rotundata uncharacterized LOC105663106 (LOC105663106), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMe108972
Clone ID Related Accession (Same CDS sequence)	XM_012290272.1 , XM_012290273.1 , XM_012290274.1 , XM_012290275.1 , XM_012290276.1 , XM_012290278.1 , XM_012290279.1 , XM_012290277.1
Accession Version	XM_012290274.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	uncharacterized protein LOC105663106 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797277.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACGG AAGCGAAGGA GCGAAGCAGA GCACAGGCAA AGAATTTGCT TTTACGTATG 
AAGCAAAAAA AAAAGGCTTA TTATTCAAAA CGTTTGTCCA AAAGAAGATT ATTGAGAAAC 
TTACGTAAGT TAAGAAACCA AAAAGCGGCC TCTATGTCAA ACGACGATCA AAATAATGAA 
GCAAATCAAC AAACTGACCA AACTAAAGTT GGCGAAATAA AAGCAGATGG TGAACACAAC 
GAAATAAAAA CAAATGCCGA ATGCAACGAA ATAAAAACAG ATGCTGAATG CAACGAAACA 
AAAGCAGATG ATTGTAGAAT TATCTGCTAC CAAAGTCTTC CACTCAATAA TAAAGGCTCT 
CGCAAGTGCA GTCTAAACTG TTTTCCTCTA TGTAAAATAT ATGAAGACTA TAAAGGAAAG 
CTCGAGGAGC TTAAAATTAT TAAAGGAAGT ATGGAACTAA TCGACTTCAA TGAAGAGGAA 
CAACGTGAAA TGAGCAGCAT CAACGGAACT ATGCAAGTAT ACGCTATAAT ACCGAAAGAG 
AATGAAAAAG ATATATTGGA TGAAACGCAA GAAACATCAA ATCTAAATAA TGATTACGAG 
GCTTTAAATC AAGAAATTAT GTCTTTAAGC CAAGAGACTA TACCTTCGAG TCAGGAAACT 
ATTTCGGATG ATACTGACCT CGAATGCAAT TGGATAGTAG AAAAAGCAAA ACCGCAAACG 
CAGGAACAAC CAATTTTGGA AGGAATAGAT TTTGTCGATT TTAAATATGT TTTTAATCAA 
TTATTAACAC TCGACGATCA TTCCAACATC GGATGCAGCA TTCGACATTT TAAAATAATT 
GGACGTTACC GGAATGGACT GAAAATAACA TATTCTGTAA AATGTGAAAT GTGCAATCAG 
ATAATACATA TACCATGCAT TCAACAGAAT AATGACGTTA TAGATATCAA TGAGAGCGCT 
GTTTCCGCGA CAATGGTATT AGGCGGCGGA TATGTTCAAC TGATGGAGAT GTTGGCTTGT 
CTGAATGTGT CGTGTATGTC CAAACAATCA TACGACAAAC ACAGAACAAA AATAATAGAG 
GCCTTCGAAC TGGCTGCACA GTGGGAAATG GAAGCTGCTG GTAAAAAAGA GAGAGAACTA 
GCTATAGCAA GAGGTGATGT ATGTCCAGAC TCTGGCATAC CATGGATTCC AGTTGTCGCT 
GATGGTAGCT GGATGAAAAG ATCATACAAA GGTGGTTATT ATAATTCTCC TTCCGGTGTA 
GGAGTTATTG TTGGATATTA TACAAAGAAA GTGTTATATG TTGGCGTAAA GAATAAAGTC 
TGCATAACTT GCCGGGTAGC GGCTAAGAAA AAAGAAAAAC CTAGCGAGCA TCGTTGCTTC 
AAAAATTGGG GTACAACTCA GAGTTCAACT GCAATGGAAA GCGTTGCTAT TTTGGAGGGA 
TTTGAATGCA GCATAAAAAT GCACGGCCTG ATATATTCAA TATTAATTGC CGATGGTGAT 
AGCAGTGTCT ACAAAAAGAT ATTGGACCGT GATCCGTATA AACAGTACTC GGTGCGTGTA 
CAAAAAATTG AATGTATCAA TCACATGCTA CGCAATTTCG GTAAGAAAAT TGCTAATATC 
GGCACTACAG GTGGGTGCCA AAAATTAAGA GCTGTCGTAA AAAAAAACGC TCTCCGTCTG 
CGCGCTTGTA TAAGGAAAGC TGCACAATAC CGCTATAACG AAAACCTGCC ACTGGTTGAA 
AAACTTGAAA GACTAAAAGC AGATTTAACT AACGTGCCTA GCCACGTTTT CGGAGAACAC 
AAGGACTGTG AAAGATTGGC ATATTTCTGC AACCAGTACT CTGCTCCCAA CGTAATAAAC 
TATGTTCCGC AACTGAAGGA AGCAGGTATT TATAAACGTA TAGAGGAAGC CATGCAGCCG 
CTTTTCAAGA ATATTCAAAG TTTGCTGTAC TGTATGAATA ATAATGCAGC AGAAGTCTTT 
AATGCCATCA TCGCGAAATA CATTGGTGGC AAACGAATAA ACTTTGGATT AAAAGGCTCG 
TATCAGGGTA GGGTTTACGC TGCTGTCGTT CAGTACAATA CAAAAGAGGC AGTGAGCCGA 
ATGTGTAGAG CAATAAATAA GGAACCGCCA GTCCTTGTCA GGAAGATTGA AGAACGACGT 
AAAAGCGAGA ATAGAAGATA TTATATATCT TCCAGGCGGC GTAAAAGACA AATGTGTGAT 
AAGGATTACG GTCCTTGTGC GGAACAACCT GAGATAGAAG AAATAGTTTT CACTCCCGAG 
TATGAGAAAA AAATAGCAGC ACTCCACAAA TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012145664.1
CDS	241..2556
Translation	MDTEAKERSRAQAKNLLLRMKQKKKAYYSKRLSKRRLLRNLRKLRNQKAASMSNDDQNNEANQQTDQTKVGEIKADGEHNEIKTNAECNEIKTDAECNETKADDCRIICYQSLPLNNKGSRKCSLNCFPLCKIYEDYKGKLEELKIIKGSMELIDFNEEEQREMSSINGTMQVYAIIPKENEKDILDETQETSNLNNDYEALNQEIMSLSQETIPSSQETISDDTDLECNWIVEKAKPQTQEQPILEGIDFVDFKYVFNQLLTLDDHSNIGCSIRHFKIIGRYRNGLKITYSVKCEMCNQIIHIPCIQQNNDVIDINESAVSATMVLGGGYVQLMEMLACLNVSCMSKQSYDKHRTKIIEAFELAAQWEMEAAGKKERELAIARGDVCPDSGIPWIPVVADGSWMKRSYKGGYYNSPSGVGVIVGYYTKKVLYVGVKNKVCITCRVAAKKKEKPSEHRCFKNWGTTQSSTAMESVAILEGFECSIKMHGLIYSILIADGDSSVYKKILDRDPYKQYSVRVQKIECINHMLRNFGKKIANIGTTGGCQKLRAVVKKNALRLRACIRKAAQYRYNENLPLVEKLERLKADLTNVPSHVFGEHKDCERLAYFCNQYSAPNVINYVPQLKEAGIYKRIEEAMQPLFKNIQSLLYCMNNNAAEVFNAIIAKYIGGKRINFGLKGSYQGRVYAAVVQYNTKEAVSRMCRAINKEPPVLVRKIEERRKSENRRYYISSRRRKRQMCDKDYGPCAEQPEIEEIVFTPEYEKKIAALHKC

Target ORF information:

RefSeq Version	XM_012290274.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata uncharacterized LOC105663106 (LOC105663106), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012290274.1

ORF Insert Sequence:

ATGGACACGG AAGCGAAGGA GCGAAGCAGA GCACAGGCAA AGAATTTGCT TTTACGTATG 
AAGCAAAAAA AAAAGGCTTA TTATTCAAAA CGTTTGTCCA AAAGAAGATT ATTGAGAAAC 
TTACGTAAGT TAAGAAACCA AAAAGCGGCC TCTATGTCAA ACGACGATCA AAATAATGAA 
GCAAATCAAC AAACTGACCA AACTAAAGTT GGCGAAATAA AAGCAGATGG TGAACACAAC 
GAAATAAAAA CAAATGCCGA ATGCAACGAA ATAAAAACAG ATGCTGAATG CAACGAAACA 
AAAGCAGATG ATTGTAGAAT TATCTGCTAC CAAAGTCTTC CACTCAATAA TAAAGGCTCT 
CGCAAGTGCA GTCTAAACTG TTTTCCTCTA TGTAAAATAT ATGAAGACTA TAAAGGAAAG 
CTCGAGGAGC TTAAAATTAT TAAAGGAAGT ATGGAACTAA TCGACTTCAA TGAAGAGGAA 
CAACGTGAAA TGAGCAGCAT CAACGGAACT ATGCAAGTAT ACGCTATAAT ACCGAAAGAG 
AATGAAAAAG ATATATTGGA TGAAACGCAA GAAACATCAA ATCTAAATAA TGATTACGAG 
GCTTTAAATC AAGAAATTAT GTCTTTAAGC CAAGAGACTA TACCTTCGAG TCAGGAAACT 
ATTTCGGATG ATACTGACCT CGAATGCAAT TGGATAGTAG AAAAAGCAAA ACCGCAAACG 
CAGGAACAAC CAATTTTGGA AGGAATAGAT TTTGTCGATT TTAAATATGT TTTTAATCAA 
TTATTAACAC TCGACGATCA TTCCAACATC GGATGCAGCA TTCGACATTT TAAAATAATT 
GGACGTTACC GGAATGGACT GAAAATAACA TATTCTGTAA AATGTGAAAT GTGCAATCAG 
ATAATACATA TACCATGCAT TCAACAGAAT AATGACGTTA TAGATATCAA TGAGAGCGCT 
GTTTCCGCGA CAATGGTATT AGGCGGCGGA TATGTTCAAC TGATGGAGAT GTTGGCTTGT 
CTGAATGTGT CGTGTATGTC CAAACAATCA TACGACAAAC ACAGAACAAA AATAATAGAG 
GCCTTCGAAC TGGCTGCACA GTGGGAAATG GAAGCTGCTG GTAAAAAAGA GAGAGAACTA 
GCTATAGCAA GAGGTGATGT ATGTCCAGAC TCTGGCATAC CATGGATTCC AGTTGTCGCT 
GATGGTAGCT GGATGAAAAG ATCATACAAA GGTGGTTATT ATAATTCTCC TTCCGGTGTA 
GGAGTTATTG TTGGATATTA TACAAAGAAA GTGTTATATG TTGGCGTAAA GAATAAAGTC 
TGCATAACTT GCCGGGTAGC GGCTAAGAAA AAAGAAAAAC CTAGCGAGCA TCGTTGCTTC 
AAAAATTGGG GTACAACTCA GAGTTCAACT GCAATGGAAA GCGTTGCTAT TTTGGAGGGA 
TTTGAATGCA GCATAAAAAT GCACGGCCTG ATATATTCAA TATTAATTGC CGATGGTGAT 
AGCAGTGTCT ACAAAAAGAT ATTGGACCGT GATCCGTATA AACAGTACTC GGTGCGTGTA 
CAAAAAATTG AATGTATCAA TCACATGCTA CGCAATTTCG GTAAGAAAAT TGCTAATATC 
GGCACTACAG GTGGGTGCCA AAAATTAAGA GCTGTCGTAA AAAAAAACGC TCTCCGTCTG 
CGCGCTTGTA TAAGGAAAGC TGCACAATAC CGCTATAACG AAAACCTGCC ACTGGTTGAA 
AAACTTGAAA GACTAAAAGC AGATTTAACT AACGTGCCTA GCCACGTTTT CGGAGAACAC 
AAGGACTGTG AAAGATTGGC ATATTTCTGC AACCAGTACT CTGCTCCCAA CGTAATAAAC 
TATGTTCCGC AACTGAAGGA AGCAGGTATT TATAAACGTA TAGAGGAAGC CATGCAGCCG 
CTTTTCAAGA ATATTCAAAG TTTGCTGTAC TGTATGAATA ATAATGCAGC AGAAGTCTTT 
AATGCCATCA TCGCGAAATA CATTGGTGGC AAACGAATAA ACTTTGGATT AAAAGGCTCG 
TATCAGGGTA GGGTTTACGC TGCTGTCGTT CAGTACAATA CAAAAGAGGC AGTGAGCCGA 
ATGTGTAGAG CAATAAATAA GGAACCGCCA GTCCTTGTCA GGAAGATTGA AGAACGACGT 
AAAAGCGAGA ATAGAAGATA TTATATATCT TCCAGGCGGC GTAAAAGACA AATGTGTGAT 
AAGGATTACG GTCCTTGTGC GGAACAACCT GAGATAGAAG AAATAGTTTT CACTCCCGAG 
TATGAGAAAA AAATAGCAGC ACTCCACAAA TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105663106 ( XM_012290275.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012145665.1 Megachile rotundata uncharacterized LOC105663106 (LOC105663106), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMe108972
Clone ID Related Accession (Same CDS sequence)	XM_012290272.1 , XM_012290273.1 , XM_012290274.1 , XM_012290275.1 , XM_012290276.1 , XM_012290278.1 , XM_012290279.1 , XM_012290277.1
Accession Version	XM_012290275.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	uncharacterized protein LOC105663106 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797277.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACGG AAGCGAAGGA GCGAAGCAGA GCACAGGCAA AGAATTTGCT TTTACGTATG 
AAGCAAAAAA AAAAGGCTTA TTATTCAAAA CGTTTGTCCA AAAGAAGATT ATTGAGAAAC 
TTACGTAAGT TAAGAAACCA AAAAGCGGCC TCTATGTCAA ACGACGATCA AAATAATGAA 
GCAAATCAAC AAACTGACCA AACTAAAGTT GGCGAAATAA AAGCAGATGG TGAACACAAC 
GAAATAAAAA CAAATGCCGA ATGCAACGAA ATAAAAACAG ATGCTGAATG CAACGAAACA 
AAAGCAGATG ATTGTAGAAT TATCTGCTAC CAAAGTCTTC CACTCAATAA TAAAGGCTCT 
CGCAAGTGCA GTCTAAACTG TTTTCCTCTA TGTAAAATAT ATGAAGACTA TAAAGGAAAG 
CTCGAGGAGC TTAAAATTAT TAAAGGAAGT ATGGAACTAA TCGACTTCAA TGAAGAGGAA 
CAACGTGAAA TGAGCAGCAT CAACGGAACT ATGCAAGTAT ACGCTATAAT ACCGAAAGAG 
AATGAAAAAG ATATATTGGA TGAAACGCAA GAAACATCAA ATCTAAATAA TGATTACGAG 
GCTTTAAATC AAGAAATTAT GTCTTTAAGC CAAGAGACTA TACCTTCGAG TCAGGAAACT 
ATTTCGGATG ATACTGACCT CGAATGCAAT TGGATAGTAG AAAAAGCAAA ACCGCAAACG 
CAGGAACAAC CAATTTTGGA AGGAATAGAT TTTGTCGATT TTAAATATGT TTTTAATCAA 
TTATTAACAC TCGACGATCA TTCCAACATC GGATGCAGCA TTCGACATTT TAAAATAATT 
GGACGTTACC GGAATGGACT GAAAATAACA TATTCTGTAA AATGTGAAAT GTGCAATCAG 
ATAATACATA TACCATGCAT TCAACAGAAT AATGACGTTA TAGATATCAA TGAGAGCGCT 
GTTTCCGCGA CAATGGTATT AGGCGGCGGA TATGTTCAAC TGATGGAGAT GTTGGCTTGT 
CTGAATGTGT CGTGTATGTC CAAACAATCA TACGACAAAC ACAGAACAAA AATAATAGAG 
GCCTTCGAAC TGGCTGCACA GTGGGAAATG GAAGCTGCTG GTAAAAAAGA GAGAGAACTA 
GCTATAGCAA GAGGTGATGT ATGTCCAGAC TCTGGCATAC CATGGATTCC AGTTGTCGCT 
GATGGTAGCT GGATGAAAAG ATCATACAAA GGTGGTTATT ATAATTCTCC TTCCGGTGTA 
GGAGTTATTG TTGGATATTA TACAAAGAAA GTGTTATATG TTGGCGTAAA GAATAAAGTC 
TGCATAACTT GCCGGGTAGC GGCTAAGAAA AAAGAAAAAC CTAGCGAGCA TCGTTGCTTC 
AAAAATTGGG GTACAACTCA GAGTTCAACT GCAATGGAAA GCGTTGCTAT TTTGGAGGGA 
TTTGAATGCA GCATAAAAAT GCACGGCCTG ATATATTCAA TATTAATTGC CGATGGTGAT 
AGCAGTGTCT ACAAAAAGAT ATTGGACCGT GATCCGTATA AACAGTACTC GGTGCGTGTA 
CAAAAAATTG AATGTATCAA TCACATGCTA CGCAATTTCG GTAAGAAAAT TGCTAATATC 
GGCACTACAG GTGGGTGCCA AAAATTAAGA GCTGTCGTAA AAAAAAACGC TCTCCGTCTG 
CGCGCTTGTA TAAGGAAAGC TGCACAATAC CGCTATAACG AAAACCTGCC ACTGGTTGAA 
AAACTTGAAA GACTAAAAGC AGATTTAACT AACGTGCCTA GCCACGTTTT CGGAGAACAC 
AAGGACTGTG AAAGATTGGC ATATTTCTGC AACCAGTACT CTGCTCCCAA CGTAATAAAC 
TATGTTCCGC AACTGAAGGA AGCAGGTATT TATAAACGTA TAGAGGAAGC CATGCAGCCG 
CTTTTCAAGA ATATTCAAAG TTTGCTGTAC TGTATGAATA ATAATGCAGC AGAAGTCTTT 
AATGCCATCA TCGCGAAATA CATTGGTGGC AAACGAATAA ACTTTGGATT AAAAGGCTCG 
TATCAGGGTA GGGTTTACGC TGCTGTCGTT CAGTACAATA CAAAAGAGGC AGTGAGCCGA 
ATGTGTAGAG CAATAAATAA GGAACCGCCA GTCCTTGTCA GGAAGATTGA AGAACGACGT 
AAAAGCGAGA ATAGAAGATA TTATATATCT TCCAGGCGGC GTAAAAGACA AATGTGTGAT 
AAGGATTACG GTCCTTGTGC GGAACAACCT GAGATAGAAG AAATAGTTTT CACTCCCGAG 
TATGAGAAAA AAATAGCAGC ACTCCACAAA TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012145665.1
CDS	241..2556
Translation	MDTEAKERSRAQAKNLLLRMKQKKKAYYSKRLSKRRLLRNLRKLRNQKAASMSNDDQNNEANQQTDQTKVGEIKADGEHNEIKTNAECNEIKTDAECNETKADDCRIICYQSLPLNNKGSRKCSLNCFPLCKIYEDYKGKLEELKIIKGSMELIDFNEEEQREMSSINGTMQVYAIIPKENEKDILDETQETSNLNNDYEALNQEIMSLSQETIPSSQETISDDTDLECNWIVEKAKPQTQEQPILEGIDFVDFKYVFNQLLTLDDHSNIGCSIRHFKIIGRYRNGLKITYSVKCEMCNQIIHIPCIQQNNDVIDINESAVSATMVLGGGYVQLMEMLACLNVSCMSKQSYDKHRTKIIEAFELAAQWEMEAAGKKERELAIARGDVCPDSGIPWIPVVADGSWMKRSYKGGYYNSPSGVGVIVGYYTKKVLYVGVKNKVCITCRVAAKKKEKPSEHRCFKNWGTTQSSTAMESVAILEGFECSIKMHGLIYSILIADGDSSVYKKILDRDPYKQYSVRVQKIECINHMLRNFGKKIANIGTTGGCQKLRAVVKKNALRLRACIRKAAQYRYNENLPLVEKLERLKADLTNVPSHVFGEHKDCERLAYFCNQYSAPNVINYVPQLKEAGIYKRIEEAMQPLFKNIQSLLYCMNNNAAEVFNAIIAKYIGGKRINFGLKGSYQGRVYAAVVQYNTKEAVSRMCRAINKEPPVLVRKIEERRKSENRRYYISSRRRKRQMCDKDYGPCAEQPEIEEIVFTPEYEKKIAALHKC

Target ORF information:

RefSeq Version	XM_012290275.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata uncharacterized LOC105663106 (LOC105663106), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012290275.1

ORF Insert Sequence:

ATGGACACGG AAGCGAAGGA GCGAAGCAGA GCACAGGCAA AGAATTTGCT TTTACGTATG 
AAGCAAAAAA AAAAGGCTTA TTATTCAAAA CGTTTGTCCA AAAGAAGATT ATTGAGAAAC 
TTACGTAAGT TAAGAAACCA AAAAGCGGCC TCTATGTCAA ACGACGATCA AAATAATGAA 
GCAAATCAAC AAACTGACCA AACTAAAGTT GGCGAAATAA AAGCAGATGG TGAACACAAC 
GAAATAAAAA CAAATGCCGA ATGCAACGAA ATAAAAACAG ATGCTGAATG CAACGAAACA 
AAAGCAGATG ATTGTAGAAT TATCTGCTAC CAAAGTCTTC CACTCAATAA TAAAGGCTCT 
CGCAAGTGCA GTCTAAACTG TTTTCCTCTA TGTAAAATAT ATGAAGACTA TAAAGGAAAG 
CTCGAGGAGC TTAAAATTAT TAAAGGAAGT ATGGAACTAA TCGACTTCAA TGAAGAGGAA 
CAACGTGAAA TGAGCAGCAT CAACGGAACT ATGCAAGTAT ACGCTATAAT ACCGAAAGAG 
AATGAAAAAG ATATATTGGA TGAAACGCAA GAAACATCAA ATCTAAATAA TGATTACGAG 
GCTTTAAATC AAGAAATTAT GTCTTTAAGC CAAGAGACTA TACCTTCGAG TCAGGAAACT 
ATTTCGGATG ATACTGACCT CGAATGCAAT TGGATAGTAG AAAAAGCAAA ACCGCAAACG 
CAGGAACAAC CAATTTTGGA AGGAATAGAT TTTGTCGATT TTAAATATGT TTTTAATCAA 
TTATTAACAC TCGACGATCA TTCCAACATC GGATGCAGCA TTCGACATTT TAAAATAATT 
GGACGTTACC GGAATGGACT GAAAATAACA TATTCTGTAA AATGTGAAAT GTGCAATCAG 
ATAATACATA TACCATGCAT TCAACAGAAT AATGACGTTA TAGATATCAA TGAGAGCGCT 
GTTTCCGCGA CAATGGTATT AGGCGGCGGA TATGTTCAAC TGATGGAGAT GTTGGCTTGT 
CTGAATGTGT CGTGTATGTC CAAACAATCA TACGACAAAC ACAGAACAAA AATAATAGAG 
GCCTTCGAAC TGGCTGCACA GTGGGAAATG GAAGCTGCTG GTAAAAAAGA GAGAGAACTA 
GCTATAGCAA GAGGTGATGT ATGTCCAGAC TCTGGCATAC CATGGATTCC AGTTGTCGCT 
GATGGTAGCT GGATGAAAAG ATCATACAAA GGTGGTTATT ATAATTCTCC TTCCGGTGTA 
GGAGTTATTG TTGGATATTA TACAAAGAAA GTGTTATATG TTGGCGTAAA GAATAAAGTC 
TGCATAACTT GCCGGGTAGC GGCTAAGAAA AAAGAAAAAC CTAGCGAGCA TCGTTGCTTC 
AAAAATTGGG GTACAACTCA GAGTTCAACT GCAATGGAAA GCGTTGCTAT TTTGGAGGGA 
TTTGAATGCA GCATAAAAAT GCACGGCCTG ATATATTCAA TATTAATTGC CGATGGTGAT 
AGCAGTGTCT ACAAAAAGAT ATTGGACCGT GATCCGTATA AACAGTACTC GGTGCGTGTA 
CAAAAAATTG AATGTATCAA TCACATGCTA CGCAATTTCG GTAAGAAAAT TGCTAATATC 
GGCACTACAG GTGGGTGCCA AAAATTAAGA GCTGTCGTAA AAAAAAACGC TCTCCGTCTG 
CGCGCTTGTA TAAGGAAAGC TGCACAATAC CGCTATAACG AAAACCTGCC ACTGGTTGAA 
AAACTTGAAA GACTAAAAGC AGATTTAACT AACGTGCCTA GCCACGTTTT CGGAGAACAC 
AAGGACTGTG AAAGATTGGC ATATTTCTGC AACCAGTACT CTGCTCCCAA CGTAATAAAC 
TATGTTCCGC AACTGAAGGA AGCAGGTATT TATAAACGTA TAGAGGAAGC CATGCAGCCG 
CTTTTCAAGA ATATTCAAAG TTTGCTGTAC TGTATGAATA ATAATGCAGC AGAAGTCTTT 
AATGCCATCA TCGCGAAATA CATTGGTGGC AAACGAATAA ACTTTGGATT AAAAGGCTCG 
TATCAGGGTA GGGTTTACGC TGCTGTCGTT CAGTACAATA CAAAAGAGGC AGTGAGCCGA 
ATGTGTAGAG CAATAAATAA GGAACCGCCA GTCCTTGTCA GGAAGATTGA AGAACGACGT 
AAAAGCGAGA ATAGAAGATA TTATATATCT TCCAGGCGGC GTAAAAGACA AATGTGTGAT 
AAGGATTACG GTCCTTGTGC GGAACAACCT GAGATAGAAG AAATAGTTTT CACTCCCGAG 
TATGAGAAAA AAATAGCAGC ACTCCACAAA TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105663106 ( XM_012290276.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012145666.1 Megachile rotundata uncharacterized LOC105663106 (LOC105663106), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMe108972
Clone ID Related Accession (Same CDS sequence)	XM_012290272.1 , XM_012290273.1 , XM_012290274.1 , XM_012290275.1 , XM_012290276.1 , XM_012290278.1 , XM_012290279.1 , XM_012290277.1
Accession Version	XM_012290276.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	uncharacterized protein LOC105663106 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797277.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACGG AAGCGAAGGA GCGAAGCAGA GCACAGGCAA AGAATTTGCT TTTACGTATG 
AAGCAAAAAA AAAAGGCTTA TTATTCAAAA CGTTTGTCCA AAAGAAGATT ATTGAGAAAC 
TTACGTAAGT TAAGAAACCA AAAAGCGGCC TCTATGTCAA ACGACGATCA AAATAATGAA 
GCAAATCAAC AAACTGACCA AACTAAAGTT GGCGAAATAA AAGCAGATGG TGAACACAAC 
GAAATAAAAA CAAATGCCGA ATGCAACGAA ATAAAAACAG ATGCTGAATG CAACGAAACA 
AAAGCAGATG ATTGTAGAAT TATCTGCTAC CAAAGTCTTC CACTCAATAA TAAAGGCTCT 
CGCAAGTGCA GTCTAAACTG TTTTCCTCTA TGTAAAATAT ATGAAGACTA TAAAGGAAAG 
CTCGAGGAGC TTAAAATTAT TAAAGGAAGT ATGGAACTAA TCGACTTCAA TGAAGAGGAA 
CAACGTGAAA TGAGCAGCAT CAACGGAACT ATGCAAGTAT ACGCTATAAT ACCGAAAGAG 
AATGAAAAAG ATATATTGGA TGAAACGCAA GAAACATCAA ATCTAAATAA TGATTACGAG 
GCTTTAAATC AAGAAATTAT GTCTTTAAGC CAAGAGACTA TACCTTCGAG TCAGGAAACT 
ATTTCGGATG ATACTGACCT CGAATGCAAT TGGATAGTAG AAAAAGCAAA ACCGCAAACG 
CAGGAACAAC CAATTTTGGA AGGAATAGAT TTTGTCGATT TTAAATATGT TTTTAATCAA 
TTATTAACAC TCGACGATCA TTCCAACATC GGATGCAGCA TTCGACATTT TAAAATAATT 
GGACGTTACC GGAATGGACT GAAAATAACA TATTCTGTAA AATGTGAAAT GTGCAATCAG 
ATAATACATA TACCATGCAT TCAACAGAAT AATGACGTTA TAGATATCAA TGAGAGCGCT 
GTTTCCGCGA CAATGGTATT AGGCGGCGGA TATGTTCAAC TGATGGAGAT GTTGGCTTGT 
CTGAATGTGT CGTGTATGTC CAAACAATCA TACGACAAAC ACAGAACAAA AATAATAGAG 
GCCTTCGAAC TGGCTGCACA GTGGGAAATG GAAGCTGCTG GTAAAAAAGA GAGAGAACTA 
GCTATAGCAA GAGGTGATGT ATGTCCAGAC TCTGGCATAC CATGGATTCC AGTTGTCGCT 
GATGGTAGCT GGATGAAAAG ATCATACAAA GGTGGTTATT ATAATTCTCC TTCCGGTGTA 
GGAGTTATTG TTGGATATTA TACAAAGAAA GTGTTATATG TTGGCGTAAA GAATAAAGTC 
TGCATAACTT GCCGGGTAGC GGCTAAGAAA AAAGAAAAAC CTAGCGAGCA TCGTTGCTTC 
AAAAATTGGG GTACAACTCA GAGTTCAACT GCAATGGAAA GCGTTGCTAT TTTGGAGGGA 
TTTGAATGCA GCATAAAAAT GCACGGCCTG ATATATTCAA TATTAATTGC CGATGGTGAT 
AGCAGTGTCT ACAAAAAGAT ATTGGACCGT GATCCGTATA AACAGTACTC GGTGCGTGTA 
CAAAAAATTG AATGTATCAA TCACATGCTA CGCAATTTCG GTAAGAAAAT TGCTAATATC 
GGCACTACAG GTGGGTGCCA AAAATTAAGA GCTGTCGTAA AAAAAAACGC TCTCCGTCTG 
CGCGCTTGTA TAAGGAAAGC TGCACAATAC CGCTATAACG AAAACCTGCC ACTGGTTGAA 
AAACTTGAAA GACTAAAAGC AGATTTAACT AACGTGCCTA GCCACGTTTT CGGAGAACAC 
AAGGACTGTG AAAGATTGGC ATATTTCTGC AACCAGTACT CTGCTCCCAA CGTAATAAAC 
TATGTTCCGC AACTGAAGGA AGCAGGTATT TATAAACGTA TAGAGGAAGC CATGCAGCCG 
CTTTTCAAGA ATATTCAAAG TTTGCTGTAC TGTATGAATA ATAATGCAGC AGAAGTCTTT 
AATGCCATCA TCGCGAAATA CATTGGTGGC AAACGAATAA ACTTTGGATT AAAAGGCTCG 
TATCAGGGTA GGGTTTACGC TGCTGTCGTT CAGTACAATA CAAAAGAGGC AGTGAGCCGA 
ATGTGTAGAG CAATAAATAA GGAACCGCCA GTCCTTGTCA GGAAGATTGA AGAACGACGT 
AAAAGCGAGA ATAGAAGATA TTATATATCT TCCAGGCGGC GTAAAAGACA AATGTGTGAT 
AAGGATTACG GTCCTTGTGC GGAACAACCT GAGATAGAAG AAATAGTTTT CACTCCCGAG 
TATGAGAAAA AAATAGCAGC ACTCCACAAA TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012145666.1
CDS	241..2556
Translation	MDTEAKERSRAQAKNLLLRMKQKKKAYYSKRLSKRRLLRNLRKLRNQKAASMSNDDQNNEANQQTDQTKVGEIKADGEHNEIKTNAECNEIKTDAECNETKADDCRIICYQSLPLNNKGSRKCSLNCFPLCKIYEDYKGKLEELKIIKGSMELIDFNEEEQREMSSINGTMQVYAIIPKENEKDILDETQETSNLNNDYEALNQEIMSLSQETIPSSQETISDDTDLECNWIVEKAKPQTQEQPILEGIDFVDFKYVFNQLLTLDDHSNIGCSIRHFKIIGRYRNGLKITYSVKCEMCNQIIHIPCIQQNNDVIDINESAVSATMVLGGGYVQLMEMLACLNVSCMSKQSYDKHRTKIIEAFELAAQWEMEAAGKKERELAIARGDVCPDSGIPWIPVVADGSWMKRSYKGGYYNSPSGVGVIVGYYTKKVLYVGVKNKVCITCRVAAKKKEKPSEHRCFKNWGTTQSSTAMESVAILEGFECSIKMHGLIYSILIADGDSSVYKKILDRDPYKQYSVRVQKIECINHMLRNFGKKIANIGTTGGCQKLRAVVKKNALRLRACIRKAAQYRYNENLPLVEKLERLKADLTNVPSHVFGEHKDCERLAYFCNQYSAPNVINYVPQLKEAGIYKRIEEAMQPLFKNIQSLLYCMNNNAAEVFNAIIAKYIGGKRINFGLKGSYQGRVYAAVVQYNTKEAVSRMCRAINKEPPVLVRKIEERRKSENRRYYISSRRRKRQMCDKDYGPCAEQPEIEEIVFTPEYEKKIAALHKC

Target ORF information:

RefSeq Version	XM_012290276.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata uncharacterized LOC105663106 (LOC105663106), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012290276.1

ORF Insert Sequence:

ATGGACACGG AAGCGAAGGA GCGAAGCAGA GCACAGGCAA AGAATTTGCT TTTACGTATG 
AAGCAAAAAA AAAAGGCTTA TTATTCAAAA CGTTTGTCCA AAAGAAGATT ATTGAGAAAC 
TTACGTAAGT TAAGAAACCA AAAAGCGGCC TCTATGTCAA ACGACGATCA AAATAATGAA 
GCAAATCAAC AAACTGACCA AACTAAAGTT GGCGAAATAA AAGCAGATGG TGAACACAAC 
GAAATAAAAA CAAATGCCGA ATGCAACGAA ATAAAAACAG ATGCTGAATG CAACGAAACA 
AAAGCAGATG ATTGTAGAAT TATCTGCTAC CAAAGTCTTC CACTCAATAA TAAAGGCTCT 
CGCAAGTGCA GTCTAAACTG TTTTCCTCTA TGTAAAATAT ATGAAGACTA TAAAGGAAAG 
CTCGAGGAGC TTAAAATTAT TAAAGGAAGT ATGGAACTAA TCGACTTCAA TGAAGAGGAA 
CAACGTGAAA TGAGCAGCAT CAACGGAACT ATGCAAGTAT ACGCTATAAT ACCGAAAGAG 
AATGAAAAAG ATATATTGGA TGAAACGCAA GAAACATCAA ATCTAAATAA TGATTACGAG 
GCTTTAAATC AAGAAATTAT GTCTTTAAGC CAAGAGACTA TACCTTCGAG TCAGGAAACT 
ATTTCGGATG ATACTGACCT CGAATGCAAT TGGATAGTAG AAAAAGCAAA ACCGCAAACG 
CAGGAACAAC CAATTTTGGA AGGAATAGAT TTTGTCGATT TTAAATATGT TTTTAATCAA 
TTATTAACAC TCGACGATCA TTCCAACATC GGATGCAGCA TTCGACATTT TAAAATAATT 
GGACGTTACC GGAATGGACT GAAAATAACA TATTCTGTAA AATGTGAAAT GTGCAATCAG 
ATAATACATA TACCATGCAT TCAACAGAAT AATGACGTTA TAGATATCAA TGAGAGCGCT 
GTTTCCGCGA CAATGGTATT AGGCGGCGGA TATGTTCAAC TGATGGAGAT GTTGGCTTGT 
CTGAATGTGT CGTGTATGTC CAAACAATCA TACGACAAAC ACAGAACAAA AATAATAGAG 
GCCTTCGAAC TGGCTGCACA GTGGGAAATG GAAGCTGCTG GTAAAAAAGA GAGAGAACTA 
GCTATAGCAA GAGGTGATGT ATGTCCAGAC TCTGGCATAC CATGGATTCC AGTTGTCGCT 
GATGGTAGCT GGATGAAAAG ATCATACAAA GGTGGTTATT ATAATTCTCC TTCCGGTGTA 
GGAGTTATTG TTGGATATTA TACAAAGAAA GTGTTATATG TTGGCGTAAA GAATAAAGTC 
TGCATAACTT GCCGGGTAGC GGCTAAGAAA AAAGAAAAAC CTAGCGAGCA TCGTTGCTTC 
AAAAATTGGG GTACAACTCA GAGTTCAACT GCAATGGAAA GCGTTGCTAT TTTGGAGGGA 
TTTGAATGCA GCATAAAAAT GCACGGCCTG ATATATTCAA TATTAATTGC CGATGGTGAT 
AGCAGTGTCT ACAAAAAGAT ATTGGACCGT GATCCGTATA AACAGTACTC GGTGCGTGTA 
CAAAAAATTG AATGTATCAA TCACATGCTA CGCAATTTCG GTAAGAAAAT TGCTAATATC 
GGCACTACAG GTGGGTGCCA AAAATTAAGA GCTGTCGTAA AAAAAAACGC TCTCCGTCTG 
CGCGCTTGTA TAAGGAAAGC TGCACAATAC CGCTATAACG AAAACCTGCC ACTGGTTGAA 
AAACTTGAAA GACTAAAAGC AGATTTAACT AACGTGCCTA GCCACGTTTT CGGAGAACAC 
AAGGACTGTG AAAGATTGGC ATATTTCTGC AACCAGTACT CTGCTCCCAA CGTAATAAAC 
TATGTTCCGC AACTGAAGGA AGCAGGTATT TATAAACGTA TAGAGGAAGC CATGCAGCCG 
CTTTTCAAGA ATATTCAAAG TTTGCTGTAC TGTATGAATA ATAATGCAGC AGAAGTCTTT 
AATGCCATCA TCGCGAAATA CATTGGTGGC AAACGAATAA ACTTTGGATT AAAAGGCTCG 
TATCAGGGTA GGGTTTACGC TGCTGTCGTT CAGTACAATA CAAAAGAGGC AGTGAGCCGA 
ATGTGTAGAG CAATAAATAA GGAACCGCCA GTCCTTGTCA GGAAGATTGA AGAACGACGT 
AAAAGCGAGA ATAGAAGATA TTATATATCT TCCAGGCGGC GTAAAAGACA AATGTGTGAT 
AAGGATTACG GTCCTTGTGC GGAACAACCT GAGATAGAAG AAATAGTTTT CACTCCCGAG 
TATGAGAAAA AAATAGCAGC ACTCCACAAA TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105663106 ( XM_012290278.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012145668.1 Megachile rotundata uncharacterized LOC105663106 (LOC105663106), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OMe108972
Clone ID Related Accession (Same CDS sequence)	XM_012290272.1 , XM_012290273.1 , XM_012290274.1 , XM_012290275.1 , XM_012290276.1 , XM_012290278.1 , XM_012290279.1 , XM_012290277.1
Accession Version	XM_012290278.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	uncharacterized protein LOC105663106 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797277.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACGG AAGCGAAGGA GCGAAGCAGA GCACAGGCAA AGAATTTGCT TTTACGTATG 
AAGCAAAAAA AAAAGGCTTA TTATTCAAAA CGTTTGTCCA AAAGAAGATT ATTGAGAAAC 
TTACGTAAGT TAAGAAACCA AAAAGCGGCC TCTATGTCAA ACGACGATCA AAATAATGAA 
GCAAATCAAC AAACTGACCA AACTAAAGTT GGCGAAATAA AAGCAGATGG TGAACACAAC 
GAAATAAAAA CAAATGCCGA ATGCAACGAA ATAAAAACAG ATGCTGAATG CAACGAAACA 
AAAGCAGATG ATTGTAGAAT TATCTGCTAC CAAAGTCTTC CACTCAATAA TAAAGGCTCT 
CGCAAGTGCA GTCTAAACTG TTTTCCTCTA TGTAAAATAT ATGAAGACTA TAAAGGAAAG 
CTCGAGGAGC TTAAAATTAT TAAAGGAAGT ATGGAACTAA TCGACTTCAA TGAAGAGGAA 
CAACGTGAAA TGAGCAGCAT CAACGGAACT ATGCAAGTAT ACGCTATAAT ACCGAAAGAG 
AATGAAAAAG ATATATTGGA TGAAACGCAA GAAACATCAA ATCTAAATAA TGATTACGAG 
GCTTTAAATC AAGAAATTAT GTCTTTAAGC CAAGAGACTA TACCTTCGAG TCAGGAAACT 
ATTTCGGATG ATACTGACCT CGAATGCAAT TGGATAGTAG AAAAAGCAAA ACCGCAAACG 
CAGGAACAAC CAATTTTGGA AGGAATAGAT TTTGTCGATT TTAAATATGT TTTTAATCAA 
TTATTAACAC TCGACGATCA TTCCAACATC GGATGCAGCA TTCGACATTT TAAAATAATT 
GGACGTTACC GGAATGGACT GAAAATAACA TATTCTGTAA AATGTGAAAT GTGCAATCAG 
ATAATACATA TACCATGCAT TCAACAGAAT AATGACGTTA TAGATATCAA TGAGAGCGCT 
GTTTCCGCGA CAATGGTATT AGGCGGCGGA TATGTTCAAC TGATGGAGAT GTTGGCTTGT 
CTGAATGTGT CGTGTATGTC CAAACAATCA TACGACAAAC ACAGAACAAA AATAATAGAG 
GCCTTCGAAC TGGCTGCACA GTGGGAAATG GAAGCTGCTG GTAAAAAAGA GAGAGAACTA 
GCTATAGCAA GAGGTGATGT ATGTCCAGAC TCTGGCATAC CATGGATTCC AGTTGTCGCT 
GATGGTAGCT GGATGAAAAG ATCATACAAA GGTGGTTATT ATAATTCTCC TTCCGGTGTA 
GGAGTTATTG TTGGATATTA TACAAAGAAA GTGTTATATG TTGGCGTAAA GAATAAAGTC 
TGCATAACTT GCCGGGTAGC GGCTAAGAAA AAAGAAAAAC CTAGCGAGCA TCGTTGCTTC 
AAAAATTGGG GTACAACTCA GAGTTCAACT GCAATGGAAA GCGTTGCTAT TTTGGAGGGA 
TTTGAATGCA GCATAAAAAT GCACGGCCTG ATATATTCAA TATTAATTGC CGATGGTGAT 
AGCAGTGTCT ACAAAAAGAT ATTGGACCGT GATCCGTATA AACAGTACTC GGTGCGTGTA 
CAAAAAATTG AATGTATCAA TCACATGCTA CGCAATTTCG GTAAGAAAAT TGCTAATATC 
GGCACTACAG GTGGGTGCCA AAAATTAAGA GCTGTCGTAA AAAAAAACGC TCTCCGTCTG 
CGCGCTTGTA TAAGGAAAGC TGCACAATAC CGCTATAACG AAAACCTGCC ACTGGTTGAA 
AAACTTGAAA GACTAAAAGC AGATTTAACT AACGTGCCTA GCCACGTTTT CGGAGAACAC 
AAGGACTGTG AAAGATTGGC ATATTTCTGC AACCAGTACT CTGCTCCCAA CGTAATAAAC 
TATGTTCCGC AACTGAAGGA AGCAGGTATT TATAAACGTA TAGAGGAAGC CATGCAGCCG 
CTTTTCAAGA ATATTCAAAG TTTGCTGTAC TGTATGAATA ATAATGCAGC AGAAGTCTTT 
AATGCCATCA TCGCGAAATA CATTGGTGGC AAACGAATAA ACTTTGGATT AAAAGGCTCG 
TATCAGGGTA GGGTTTACGC TGCTGTCGTT CAGTACAATA CAAAAGAGGC AGTGAGCCGA 
ATGTGTAGAG CAATAAATAA GGAACCGCCA GTCCTTGTCA GGAAGATTGA AGAACGACGT 
AAAAGCGAGA ATAGAAGATA TTATATATCT TCCAGGCGGC GTAAAAGACA AATGTGTGAT 
AAGGATTACG GTCCTTGTGC GGAACAACCT GAGATAGAAG AAATAGTTTT CACTCCCGAG 
TATGAGAAAA AAATAGCAGC ACTCCACAAA TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012145668.1
CDS	241..2556
Translation	MDTEAKERSRAQAKNLLLRMKQKKKAYYSKRLSKRRLLRNLRKLRNQKAASMSNDDQNNEANQQTDQTKVGEIKADGEHNEIKTNAECNEIKTDAECNETKADDCRIICYQSLPLNNKGSRKCSLNCFPLCKIYEDYKGKLEELKIIKGSMELIDFNEEEQREMSSINGTMQVYAIIPKENEKDILDETQETSNLNNDYEALNQEIMSLSQETIPSSQETISDDTDLECNWIVEKAKPQTQEQPILEGIDFVDFKYVFNQLLTLDDHSNIGCSIRHFKIIGRYRNGLKITYSVKCEMCNQIIHIPCIQQNNDVIDINESAVSATMVLGGGYVQLMEMLACLNVSCMSKQSYDKHRTKIIEAFELAAQWEMEAAGKKERELAIARGDVCPDSGIPWIPVVADGSWMKRSYKGGYYNSPSGVGVIVGYYTKKVLYVGVKNKVCITCRVAAKKKEKPSEHRCFKNWGTTQSSTAMESVAILEGFECSIKMHGLIYSILIADGDSSVYKKILDRDPYKQYSVRVQKIECINHMLRNFGKKIANIGTTGGCQKLRAVVKKNALRLRACIRKAAQYRYNENLPLVEKLERLKADLTNVPSHVFGEHKDCERLAYFCNQYSAPNVINYVPQLKEAGIYKRIEEAMQPLFKNIQSLLYCMNNNAAEVFNAIIAKYIGGKRINFGLKGSYQGRVYAAVVQYNTKEAVSRMCRAINKEPPVLVRKIEERRKSENRRYYISSRRRKRQMCDKDYGPCAEQPEIEEIVFTPEYEKKIAALHKC

Target ORF information:

RefSeq Version	XM_012290278.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata uncharacterized LOC105663106 (LOC105663106), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012290278.1

ORF Insert Sequence:

ATGGACACGG AAGCGAAGGA GCGAAGCAGA GCACAGGCAA AGAATTTGCT TTTACGTATG 
AAGCAAAAAA AAAAGGCTTA TTATTCAAAA CGTTTGTCCA AAAGAAGATT ATTGAGAAAC 
TTACGTAAGT TAAGAAACCA AAAAGCGGCC TCTATGTCAA ACGACGATCA AAATAATGAA 
GCAAATCAAC AAACTGACCA AACTAAAGTT GGCGAAATAA AAGCAGATGG TGAACACAAC 
GAAATAAAAA CAAATGCCGA ATGCAACGAA ATAAAAACAG ATGCTGAATG CAACGAAACA 
AAAGCAGATG ATTGTAGAAT TATCTGCTAC CAAAGTCTTC CACTCAATAA TAAAGGCTCT 
CGCAAGTGCA GTCTAAACTG TTTTCCTCTA TGTAAAATAT ATGAAGACTA TAAAGGAAAG 
CTCGAGGAGC TTAAAATTAT TAAAGGAAGT ATGGAACTAA TCGACTTCAA TGAAGAGGAA 
CAACGTGAAA TGAGCAGCAT CAACGGAACT ATGCAAGTAT ACGCTATAAT ACCGAAAGAG 
AATGAAAAAG ATATATTGGA TGAAACGCAA GAAACATCAA ATCTAAATAA TGATTACGAG 
GCTTTAAATC AAGAAATTAT GTCTTTAAGC CAAGAGACTA TACCTTCGAG TCAGGAAACT 
ATTTCGGATG ATACTGACCT CGAATGCAAT TGGATAGTAG AAAAAGCAAA ACCGCAAACG 
CAGGAACAAC CAATTTTGGA AGGAATAGAT TTTGTCGATT TTAAATATGT TTTTAATCAA 
TTATTAACAC TCGACGATCA TTCCAACATC GGATGCAGCA TTCGACATTT TAAAATAATT 
GGACGTTACC GGAATGGACT GAAAATAACA TATTCTGTAA AATGTGAAAT GTGCAATCAG 
ATAATACATA TACCATGCAT TCAACAGAAT AATGACGTTA TAGATATCAA TGAGAGCGCT 
GTTTCCGCGA CAATGGTATT AGGCGGCGGA TATGTTCAAC TGATGGAGAT GTTGGCTTGT 
CTGAATGTGT CGTGTATGTC CAAACAATCA TACGACAAAC ACAGAACAAA AATAATAGAG 
GCCTTCGAAC TGGCTGCACA GTGGGAAATG GAAGCTGCTG GTAAAAAAGA GAGAGAACTA 
GCTATAGCAA GAGGTGATGT ATGTCCAGAC TCTGGCATAC CATGGATTCC AGTTGTCGCT 
GATGGTAGCT GGATGAAAAG ATCATACAAA GGTGGTTATT ATAATTCTCC TTCCGGTGTA 
GGAGTTATTG TTGGATATTA TACAAAGAAA GTGTTATATG TTGGCGTAAA GAATAAAGTC 
TGCATAACTT GCCGGGTAGC GGCTAAGAAA AAAGAAAAAC CTAGCGAGCA TCGTTGCTTC 
AAAAATTGGG GTACAACTCA GAGTTCAACT GCAATGGAAA GCGTTGCTAT TTTGGAGGGA 
TTTGAATGCA GCATAAAAAT GCACGGCCTG ATATATTCAA TATTAATTGC CGATGGTGAT 
AGCAGTGTCT ACAAAAAGAT ATTGGACCGT GATCCGTATA AACAGTACTC GGTGCGTGTA 
CAAAAAATTG AATGTATCAA TCACATGCTA CGCAATTTCG GTAAGAAAAT TGCTAATATC 
GGCACTACAG GTGGGTGCCA AAAATTAAGA GCTGTCGTAA AAAAAAACGC TCTCCGTCTG 
CGCGCTTGTA TAAGGAAAGC TGCACAATAC CGCTATAACG AAAACCTGCC ACTGGTTGAA 
AAACTTGAAA GACTAAAAGC AGATTTAACT AACGTGCCTA GCCACGTTTT CGGAGAACAC 
AAGGACTGTG AAAGATTGGC ATATTTCTGC AACCAGTACT CTGCTCCCAA CGTAATAAAC 
TATGTTCCGC AACTGAAGGA AGCAGGTATT TATAAACGTA TAGAGGAAGC CATGCAGCCG 
CTTTTCAAGA ATATTCAAAG TTTGCTGTAC TGTATGAATA ATAATGCAGC AGAAGTCTTT 
AATGCCATCA TCGCGAAATA CATTGGTGGC AAACGAATAA ACTTTGGATT AAAAGGCTCG 
TATCAGGGTA GGGTTTACGC TGCTGTCGTT CAGTACAATA CAAAAGAGGC AGTGAGCCGA 
ATGTGTAGAG CAATAAATAA GGAACCGCCA GTCCTTGTCA GGAAGATTGA AGAACGACGT 
AAAAGCGAGA ATAGAAGATA TTATATATCT TCCAGGCGGC GTAAAAGACA AATGTGTGAT 
AAGGATTACG GTCCTTGTGC GGAACAACCT GAGATAGAAG AAATAGTTTT CACTCCCGAG 
TATGAGAAAA AAATAGCAGC ACTCCACAAA TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105663106 ( XM_012290279.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012145669.1 Megachile rotundata uncharacterized LOC105663106 (LOC105663106), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OMe108972
Clone ID Related Accession (Same CDS sequence)	XM_012290272.1 , XM_012290273.1 , XM_012290274.1 , XM_012290275.1 , XM_012290276.1 , XM_012290278.1 , XM_012290279.1 , XM_012290277.1
Accession Version	XM_012290279.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	uncharacterized protein LOC105663106 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797277.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACGG AAGCGAAGGA GCGAAGCAGA GCACAGGCAA AGAATTTGCT TTTACGTATG 
AAGCAAAAAA AAAAGGCTTA TTATTCAAAA CGTTTGTCCA AAAGAAGATT ATTGAGAAAC 
TTACGTAAGT TAAGAAACCA AAAAGCGGCC TCTATGTCAA ACGACGATCA AAATAATGAA 
GCAAATCAAC AAACTGACCA AACTAAAGTT GGCGAAATAA AAGCAGATGG TGAACACAAC 
GAAATAAAAA CAAATGCCGA ATGCAACGAA ATAAAAACAG ATGCTGAATG CAACGAAACA 
AAAGCAGATG ATTGTAGAAT TATCTGCTAC CAAAGTCTTC CACTCAATAA TAAAGGCTCT 
CGCAAGTGCA GTCTAAACTG TTTTCCTCTA TGTAAAATAT ATGAAGACTA TAAAGGAAAG 
CTCGAGGAGC TTAAAATTAT TAAAGGAAGT ATGGAACTAA TCGACTTCAA TGAAGAGGAA 
CAACGTGAAA TGAGCAGCAT CAACGGAACT ATGCAAGTAT ACGCTATAAT ACCGAAAGAG 
AATGAAAAAG ATATATTGGA TGAAACGCAA GAAACATCAA ATCTAAATAA TGATTACGAG 
GCTTTAAATC AAGAAATTAT GTCTTTAAGC CAAGAGACTA TACCTTCGAG TCAGGAAACT 
ATTTCGGATG ATACTGACCT CGAATGCAAT TGGATAGTAG AAAAAGCAAA ACCGCAAACG 
CAGGAACAAC CAATTTTGGA AGGAATAGAT TTTGTCGATT TTAAATATGT TTTTAATCAA 
TTATTAACAC TCGACGATCA TTCCAACATC GGATGCAGCA TTCGACATTT TAAAATAATT 
GGACGTTACC GGAATGGACT GAAAATAACA TATTCTGTAA AATGTGAAAT GTGCAATCAG 
ATAATACATA TACCATGCAT TCAACAGAAT AATGACGTTA TAGATATCAA TGAGAGCGCT 
GTTTCCGCGA CAATGGTATT AGGCGGCGGA TATGTTCAAC TGATGGAGAT GTTGGCTTGT 
CTGAATGTGT CGTGTATGTC CAAACAATCA TACGACAAAC ACAGAACAAA AATAATAGAG 
GCCTTCGAAC TGGCTGCACA GTGGGAAATG GAAGCTGCTG GTAAAAAAGA GAGAGAACTA 
GCTATAGCAA GAGGTGATGT ATGTCCAGAC TCTGGCATAC CATGGATTCC AGTTGTCGCT 
GATGGTAGCT GGATGAAAAG ATCATACAAA GGTGGTTATT ATAATTCTCC TTCCGGTGTA 
GGAGTTATTG TTGGATATTA TACAAAGAAA GTGTTATATG TTGGCGTAAA GAATAAAGTC 
TGCATAACTT GCCGGGTAGC GGCTAAGAAA AAAGAAAAAC CTAGCGAGCA TCGTTGCTTC 
AAAAATTGGG GTACAACTCA GAGTTCAACT GCAATGGAAA GCGTTGCTAT TTTGGAGGGA 
TTTGAATGCA GCATAAAAAT GCACGGCCTG ATATATTCAA TATTAATTGC CGATGGTGAT 
AGCAGTGTCT ACAAAAAGAT ATTGGACCGT GATCCGTATA AACAGTACTC GGTGCGTGTA 
CAAAAAATTG AATGTATCAA TCACATGCTA CGCAATTTCG GTAAGAAAAT TGCTAATATC 
GGCACTACAG GTGGGTGCCA AAAATTAAGA GCTGTCGTAA AAAAAAACGC TCTCCGTCTG 
CGCGCTTGTA TAAGGAAAGC TGCACAATAC CGCTATAACG AAAACCTGCC ACTGGTTGAA 
AAACTTGAAA GACTAAAAGC AGATTTAACT AACGTGCCTA GCCACGTTTT CGGAGAACAC 
AAGGACTGTG AAAGATTGGC ATATTTCTGC AACCAGTACT CTGCTCCCAA CGTAATAAAC 
TATGTTCCGC AACTGAAGGA AGCAGGTATT TATAAACGTA TAGAGGAAGC CATGCAGCCG 
CTTTTCAAGA ATATTCAAAG TTTGCTGTAC TGTATGAATA ATAATGCAGC AGAAGTCTTT 
AATGCCATCA TCGCGAAATA CATTGGTGGC AAACGAATAA ACTTTGGATT AAAAGGCTCG 
TATCAGGGTA GGGTTTACGC TGCTGTCGTT CAGTACAATA CAAAAGAGGC AGTGAGCCGA 
ATGTGTAGAG CAATAAATAA GGAACCGCCA GTCCTTGTCA GGAAGATTGA AGAACGACGT 
AAAAGCGAGA ATAGAAGATA TTATATATCT TCCAGGCGGC GTAAAAGACA AATGTGTGAT 
AAGGATTACG GTCCTTGTGC GGAACAACCT GAGATAGAAG AAATAGTTTT CACTCCCGAG 
TATGAGAAAA AAATAGCAGC ACTCCACAAA TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012145669.1
CDS	241..2556
Translation	MDTEAKERSRAQAKNLLLRMKQKKKAYYSKRLSKRRLLRNLRKLRNQKAASMSNDDQNNEANQQTDQTKVGEIKADGEHNEIKTNAECNEIKTDAECNETKADDCRIICYQSLPLNNKGSRKCSLNCFPLCKIYEDYKGKLEELKIIKGSMELIDFNEEEQREMSSINGTMQVYAIIPKENEKDILDETQETSNLNNDYEALNQEIMSLSQETIPSSQETISDDTDLECNWIVEKAKPQTQEQPILEGIDFVDFKYVFNQLLTLDDHSNIGCSIRHFKIIGRYRNGLKITYSVKCEMCNQIIHIPCIQQNNDVIDINESAVSATMVLGGGYVQLMEMLACLNVSCMSKQSYDKHRTKIIEAFELAAQWEMEAAGKKERELAIARGDVCPDSGIPWIPVVADGSWMKRSYKGGYYNSPSGVGVIVGYYTKKVLYVGVKNKVCITCRVAAKKKEKPSEHRCFKNWGTTQSSTAMESVAILEGFECSIKMHGLIYSILIADGDSSVYKKILDRDPYKQYSVRVQKIECINHMLRNFGKKIANIGTTGGCQKLRAVVKKNALRLRACIRKAAQYRYNENLPLVEKLERLKADLTNVPSHVFGEHKDCERLAYFCNQYSAPNVINYVPQLKEAGIYKRIEEAMQPLFKNIQSLLYCMNNNAAEVFNAIIAKYIGGKRINFGLKGSYQGRVYAAVVQYNTKEAVSRMCRAINKEPPVLVRKIEERRKSENRRYYISSRRRKRQMCDKDYGPCAEQPEIEEIVFTPEYEKKIAALHKC

Target ORF information:

RefSeq Version	XM_012290279.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata uncharacterized LOC105663106 (LOC105663106), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012290279.1

ORF Insert Sequence:

ATGGACACGG AAGCGAAGGA GCGAAGCAGA GCACAGGCAA AGAATTTGCT TTTACGTATG 
AAGCAAAAAA AAAAGGCTTA TTATTCAAAA CGTTTGTCCA AAAGAAGATT ATTGAGAAAC 
TTACGTAAGT TAAGAAACCA AAAAGCGGCC TCTATGTCAA ACGACGATCA AAATAATGAA 
GCAAATCAAC AAACTGACCA AACTAAAGTT GGCGAAATAA AAGCAGATGG TGAACACAAC 
GAAATAAAAA CAAATGCCGA ATGCAACGAA ATAAAAACAG ATGCTGAATG CAACGAAACA 
AAAGCAGATG ATTGTAGAAT TATCTGCTAC CAAAGTCTTC CACTCAATAA TAAAGGCTCT 
CGCAAGTGCA GTCTAAACTG TTTTCCTCTA TGTAAAATAT ATGAAGACTA TAAAGGAAAG 
CTCGAGGAGC TTAAAATTAT TAAAGGAAGT ATGGAACTAA TCGACTTCAA TGAAGAGGAA 
CAACGTGAAA TGAGCAGCAT CAACGGAACT ATGCAAGTAT ACGCTATAAT ACCGAAAGAG 
AATGAAAAAG ATATATTGGA TGAAACGCAA GAAACATCAA ATCTAAATAA TGATTACGAG 
GCTTTAAATC AAGAAATTAT GTCTTTAAGC CAAGAGACTA TACCTTCGAG TCAGGAAACT 
ATTTCGGATG ATACTGACCT CGAATGCAAT TGGATAGTAG AAAAAGCAAA ACCGCAAACG 
CAGGAACAAC CAATTTTGGA AGGAATAGAT TTTGTCGATT TTAAATATGT TTTTAATCAA 
TTATTAACAC TCGACGATCA TTCCAACATC GGATGCAGCA TTCGACATTT TAAAATAATT 
GGACGTTACC GGAATGGACT GAAAATAACA TATTCTGTAA AATGTGAAAT GTGCAATCAG 
ATAATACATA TACCATGCAT TCAACAGAAT AATGACGTTA TAGATATCAA TGAGAGCGCT 
GTTTCCGCGA CAATGGTATT AGGCGGCGGA TATGTTCAAC TGATGGAGAT GTTGGCTTGT 
CTGAATGTGT CGTGTATGTC CAAACAATCA TACGACAAAC ACAGAACAAA AATAATAGAG 
GCCTTCGAAC TGGCTGCACA GTGGGAAATG GAAGCTGCTG GTAAAAAAGA GAGAGAACTA 
GCTATAGCAA GAGGTGATGT ATGTCCAGAC TCTGGCATAC CATGGATTCC AGTTGTCGCT 
GATGGTAGCT GGATGAAAAG ATCATACAAA GGTGGTTATT ATAATTCTCC TTCCGGTGTA 
GGAGTTATTG TTGGATATTA TACAAAGAAA GTGTTATATG TTGGCGTAAA GAATAAAGTC 
TGCATAACTT GCCGGGTAGC GGCTAAGAAA AAAGAAAAAC CTAGCGAGCA TCGTTGCTTC 
AAAAATTGGG GTACAACTCA GAGTTCAACT GCAATGGAAA GCGTTGCTAT TTTGGAGGGA 
TTTGAATGCA GCATAAAAAT GCACGGCCTG ATATATTCAA TATTAATTGC CGATGGTGAT 
AGCAGTGTCT ACAAAAAGAT ATTGGACCGT GATCCGTATA AACAGTACTC GGTGCGTGTA 
CAAAAAATTG AATGTATCAA TCACATGCTA CGCAATTTCG GTAAGAAAAT TGCTAATATC 
GGCACTACAG GTGGGTGCCA AAAATTAAGA GCTGTCGTAA AAAAAAACGC TCTCCGTCTG 
CGCGCTTGTA TAAGGAAAGC TGCACAATAC CGCTATAACG AAAACCTGCC ACTGGTTGAA 
AAACTTGAAA GACTAAAAGC AGATTTAACT AACGTGCCTA GCCACGTTTT CGGAGAACAC 
AAGGACTGTG AAAGATTGGC ATATTTCTGC AACCAGTACT CTGCTCCCAA CGTAATAAAC 
TATGTTCCGC AACTGAAGGA AGCAGGTATT TATAAACGTA TAGAGGAAGC CATGCAGCCG 
CTTTTCAAGA ATATTCAAAG TTTGCTGTAC TGTATGAATA ATAATGCAGC AGAAGTCTTT 
AATGCCATCA TCGCGAAATA CATTGGTGGC AAACGAATAA ACTTTGGATT AAAAGGCTCG 
TATCAGGGTA GGGTTTACGC TGCTGTCGTT CAGTACAATA CAAAAGAGGC AGTGAGCCGA 
ATGTGTAGAG CAATAAATAA GGAACCGCCA GTCCTTGTCA GGAAGATTGA AGAACGACGT 
AAAAGCGAGA ATAGAAGATA TTATATATCT TCCAGGCGGC GTAAAAGACA AATGTGTGAT 
AAGGATTACG GTCCTTGTGC GGAACAACCT GAGATAGAAG AAATAGTTTT CACTCCCGAG 
TATGAGAAAA AAATAGCAGC ACTCCACAAA TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105663106 ( XM_012290277.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012145667.1 Megachile rotundata uncharacterized LOC105663106 (LOC105663106), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMe108972
Clone ID Related Accession (Same CDS sequence)	XM_012290272.1 , XM_012290273.1 , XM_012290274.1 , XM_012290275.1 , XM_012290276.1 , XM_012290278.1 , XM_012290279.1 , XM_012290277.1
Accession Version	XM_012290277.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	uncharacterized protein LOC105663106 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797277.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACGG AAGCGAAGGA GCGAAGCAGA GCACAGGCAA AGAATTTGCT TTTACGTATG 
AAGCAAAAAA AAAAGGCTTA TTATTCAAAA CGTTTGTCCA AAAGAAGATT ATTGAGAAAC 
TTACGTAAGT TAAGAAACCA AAAAGCGGCC TCTATGTCAA ACGACGATCA AAATAATGAA 
GCAAATCAAC AAACTGACCA AACTAAAGTT GGCGAAATAA AAGCAGATGG TGAACACAAC 
GAAATAAAAA CAAATGCCGA ATGCAACGAA ATAAAAACAG ATGCTGAATG CAACGAAACA 
AAAGCAGATG ATTGTAGAAT TATCTGCTAC CAAAGTCTTC CACTCAATAA TAAAGGCTCT 
CGCAAGTGCA GTCTAAACTG TTTTCCTCTA TGTAAAATAT ATGAAGACTA TAAAGGAAAG 
CTCGAGGAGC TTAAAATTAT TAAAGGAAGT ATGGAACTAA TCGACTTCAA TGAAGAGGAA 
CAACGTGAAA TGAGCAGCAT CAACGGAACT ATGCAAGTAT ACGCTATAAT ACCGAAAGAG 
AATGAAAAAG ATATATTGGA TGAAACGCAA GAAACATCAA ATCTAAATAA TGATTACGAG 
GCTTTAAATC AAGAAATTAT GTCTTTAAGC CAAGAGACTA TACCTTCGAG TCAGGAAACT 
ATTTCGGATG ATACTGACCT CGAATGCAAT TGGATAGTAG AAAAAGCAAA ACCGCAAACG 
CAGGAACAAC CAATTTTGGA AGGAATAGAT TTTGTCGATT TTAAATATGT TTTTAATCAA 
TTATTAACAC TCGACGATCA TTCCAACATC GGATGCAGCA TTCGACATTT TAAAATAATT 
GGACGTTACC GGAATGGACT GAAAATAACA TATTCTGTAA AATGTGAAAT GTGCAATCAG 
ATAATACATA TACCATGCAT TCAACAGAAT AATGACGTTA TAGATATCAA TGAGAGCGCT 
GTTTCCGCGA CAATGGTATT AGGCGGCGGA TATGTTCAAC TGATGGAGAT GTTGGCTTGT 
CTGAATGTGT CGTGTATGTC CAAACAATCA TACGACAAAC ACAGAACAAA AATAATAGAG 
GCCTTCGAAC TGGCTGCACA GTGGGAAATG GAAGCTGCTG GTAAAAAAGA GAGAGAACTA 
GCTATAGCAA GAGGTGATGT ATGTCCAGAC TCTGGCATAC CATGGATTCC AGTTGTCGCT 
GATGGTAGCT GGATGAAAAG ATCATACAAA GGTGGTTATT ATAATTCTCC TTCCGGTGTA 
GGAGTTATTG TTGGATATTA TACAAAGAAA GTGTTATATG TTGGCGTAAA GAATAAAGTC 
TGCATAACTT GCCGGGTAGC GGCTAAGAAA AAAGAAAAAC CTAGCGAGCA TCGTTGCTTC 
AAAAATTGGG GTACAACTCA GAGTTCAACT GCAATGGAAA GCGTTGCTAT TTTGGAGGGA 
TTTGAATGCA GCATAAAAAT GCACGGCCTG ATATATTCAA TATTAATTGC CGATGGTGAT 
AGCAGTGTCT ACAAAAAGAT ATTGGACCGT GATCCGTATA AACAGTACTC GGTGCGTGTA 
CAAAAAATTG AATGTATCAA TCACATGCTA CGCAATTTCG GTAAGAAAAT TGCTAATATC 
GGCACTACAG GTGGGTGCCA AAAATTAAGA GCTGTCGTAA AAAAAAACGC TCTCCGTCTG 
CGCGCTTGTA TAAGGAAAGC TGCACAATAC CGCTATAACG AAAACCTGCC ACTGGTTGAA 
AAACTTGAAA GACTAAAAGC AGATTTAACT AACGTGCCTA GCCACGTTTT CGGAGAACAC 
AAGGACTGTG AAAGATTGGC ATATTTCTGC AACCAGTACT CTGCTCCCAA CGTAATAAAC 
TATGTTCCGC AACTGAAGGA AGCAGGTATT TATAAACGTA TAGAGGAAGC CATGCAGCCG 
CTTTTCAAGA ATATTCAAAG TTTGCTGTAC TGTATGAATA ATAATGCAGC AGAAGTCTTT 
AATGCCATCA TCGCGAAATA CATTGGTGGC AAACGAATAA ACTTTGGATT AAAAGGCTCG 
TATCAGGGTA GGGTTTACGC TGCTGTCGTT CAGTACAATA CAAAAGAGGC AGTGAGCCGA 
ATGTGTAGAG CAATAAATAA GGAACCGCCA GTCCTTGTCA GGAAGATTGA AGAACGACGT 
AAAAGCGAGA ATAGAAGATA TTATATATCT TCCAGGCGGC GTAAAAGACA AATGTGTGAT 
AAGGATTACG GTCCTTGTGC GGAACAACCT GAGATAGAAG AAATAGTTTT CACTCCCGAG 
TATGAGAAAA AAATAGCAGC ACTCCACAAA TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012145667.1
CDS	241..2556
Translation	MDTEAKERSRAQAKNLLLRMKQKKKAYYSKRLSKRRLLRNLRKLRNQKAASMSNDDQNNEANQQTDQTKVGEIKADGEHNEIKTNAECNEIKTDAECNETKADDCRIICYQSLPLNNKGSRKCSLNCFPLCKIYEDYKGKLEELKIIKGSMELIDFNEEEQREMSSINGTMQVYAIIPKENEKDILDETQETSNLNNDYEALNQEIMSLSQETIPSSQETISDDTDLECNWIVEKAKPQTQEQPILEGIDFVDFKYVFNQLLTLDDHSNIGCSIRHFKIIGRYRNGLKITYSVKCEMCNQIIHIPCIQQNNDVIDINESAVSATMVLGGGYVQLMEMLACLNVSCMSKQSYDKHRTKIIEAFELAAQWEMEAAGKKERELAIARGDVCPDSGIPWIPVVADGSWMKRSYKGGYYNSPSGVGVIVGYYTKKVLYVGVKNKVCITCRVAAKKKEKPSEHRCFKNWGTTQSSTAMESVAILEGFECSIKMHGLIYSILIADGDSSVYKKILDRDPYKQYSVRVQKIECINHMLRNFGKKIANIGTTGGCQKLRAVVKKNALRLRACIRKAAQYRYNENLPLVEKLERLKADLTNVPSHVFGEHKDCERLAYFCNQYSAPNVINYVPQLKEAGIYKRIEEAMQPLFKNIQSLLYCMNNNAAEVFNAIIAKYIGGKRINFGLKGSYQGRVYAAVVQYNTKEAVSRMCRAINKEPPVLVRKIEERRKSENRRYYISSRRRKRQMCDKDYGPCAEQPEIEEIVFTPEYEKKIAALHKC

Target ORF information:

RefSeq Version	XM_012290277.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata uncharacterized LOC105663106 (LOC105663106), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012290277.1

ORF Insert Sequence:

ATGGACACGG AAGCGAAGGA GCGAAGCAGA GCACAGGCAA AGAATTTGCT TTTACGTATG 
AAGCAAAAAA AAAAGGCTTA TTATTCAAAA CGTTTGTCCA AAAGAAGATT ATTGAGAAAC 
TTACGTAAGT TAAGAAACCA AAAAGCGGCC TCTATGTCAA ACGACGATCA AAATAATGAA 
GCAAATCAAC AAACTGACCA AACTAAAGTT GGCGAAATAA AAGCAGATGG TGAACACAAC 
GAAATAAAAA CAAATGCCGA ATGCAACGAA ATAAAAACAG ATGCTGAATG CAACGAAACA 
AAAGCAGATG ATTGTAGAAT TATCTGCTAC CAAAGTCTTC CACTCAATAA TAAAGGCTCT 
CGCAAGTGCA GTCTAAACTG TTTTCCTCTA TGTAAAATAT ATGAAGACTA TAAAGGAAAG 
CTCGAGGAGC TTAAAATTAT TAAAGGAAGT ATGGAACTAA TCGACTTCAA TGAAGAGGAA 
CAACGTGAAA TGAGCAGCAT CAACGGAACT ATGCAAGTAT ACGCTATAAT ACCGAAAGAG 
AATGAAAAAG ATATATTGGA TGAAACGCAA GAAACATCAA ATCTAAATAA TGATTACGAG 
GCTTTAAATC AAGAAATTAT GTCTTTAAGC CAAGAGACTA TACCTTCGAG TCAGGAAACT 
ATTTCGGATG ATACTGACCT CGAATGCAAT TGGATAGTAG AAAAAGCAAA ACCGCAAACG 
CAGGAACAAC CAATTTTGGA AGGAATAGAT TTTGTCGATT TTAAATATGT TTTTAATCAA 
TTATTAACAC TCGACGATCA TTCCAACATC GGATGCAGCA TTCGACATTT TAAAATAATT 
GGACGTTACC GGAATGGACT GAAAATAACA TATTCTGTAA AATGTGAAAT GTGCAATCAG 
ATAATACATA TACCATGCAT TCAACAGAAT AATGACGTTA TAGATATCAA TGAGAGCGCT 
GTTTCCGCGA CAATGGTATT AGGCGGCGGA TATGTTCAAC TGATGGAGAT GTTGGCTTGT 
CTGAATGTGT CGTGTATGTC CAAACAATCA TACGACAAAC ACAGAACAAA AATAATAGAG 
GCCTTCGAAC TGGCTGCACA GTGGGAAATG GAAGCTGCTG GTAAAAAAGA GAGAGAACTA 
GCTATAGCAA GAGGTGATGT ATGTCCAGAC TCTGGCATAC CATGGATTCC AGTTGTCGCT 
GATGGTAGCT GGATGAAAAG ATCATACAAA GGTGGTTATT ATAATTCTCC TTCCGGTGTA 
GGAGTTATTG TTGGATATTA TACAAAGAAA GTGTTATATG TTGGCGTAAA GAATAAAGTC 
TGCATAACTT GCCGGGTAGC GGCTAAGAAA AAAGAAAAAC CTAGCGAGCA TCGTTGCTTC 
AAAAATTGGG GTACAACTCA GAGTTCAACT GCAATGGAAA GCGTTGCTAT TTTGGAGGGA 
TTTGAATGCA GCATAAAAAT GCACGGCCTG ATATATTCAA TATTAATTGC CGATGGTGAT 
AGCAGTGTCT ACAAAAAGAT ATTGGACCGT GATCCGTATA AACAGTACTC GGTGCGTGTA 
CAAAAAATTG AATGTATCAA TCACATGCTA CGCAATTTCG GTAAGAAAAT TGCTAATATC 
GGCACTACAG GTGGGTGCCA AAAATTAAGA GCTGTCGTAA AAAAAAACGC TCTCCGTCTG 
CGCGCTTGTA TAAGGAAAGC TGCACAATAC CGCTATAACG AAAACCTGCC ACTGGTTGAA 
AAACTTGAAA GACTAAAAGC AGATTTAACT AACGTGCCTA GCCACGTTTT CGGAGAACAC 
AAGGACTGTG AAAGATTGGC ATATTTCTGC AACCAGTACT CTGCTCCCAA CGTAATAAAC 
TATGTTCCGC AACTGAAGGA AGCAGGTATT TATAAACGTA TAGAGGAAGC CATGCAGCCG 
CTTTTCAAGA ATATTCAAAG TTTGCTGTAC TGTATGAATA ATAATGCAGC AGAAGTCTTT 
AATGCCATCA TCGCGAAATA CATTGGTGGC AAACGAATAA ACTTTGGATT AAAAGGCTCG 
TATCAGGGTA GGGTTTACGC TGCTGTCGTT CAGTACAATA CAAAAGAGGC AGTGAGCCGA 
ATGTGTAGAG CAATAAATAA GGAACCGCCA GTCCTTGTCA GGAAGATTGA AGAACGACGT 
AAAAGCGAGA ATAGAAGATA TTATATATCT TCCAGGCGGC GTAAAAGACA AATGTGTGAT 
AAGGATTACG GTCCTTGTGC GGAACAACCT GAGATAGAAG AAATAGTTTT CACTCCCGAG 
TATGAGAAAA AAATAGCAGC ACTCCACAAA TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105663106 ( XM_012290280.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012145670.1 Megachile rotundata uncharacterized LOC105663106 (LOC105663106), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OMe108973
Clone ID Related Accession (Same CDS sequence)	XM_012290280.1
Accession Version	XM_012290280.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	uncharacterized protein LOC105663106 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797277.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAATCA ACGGATTTGA CGGTCTAGAA CCTTGGAAAT TCAAGGATTC GAGAATACGT 
AAGTTAAGAA ACCAAAAAGC GGCCTCTATG TCAAACGACG ATCAAAATAA TGAAGCAAAT 
CAACAAACTG ACCAAACTAA AGTTGGCGAA ATAAAAGCAG ATGGTGAACA CAACGAAATA 
AAAACAAATG CCGAATGCAA CGAAATAAAA ACAGATGCTG AATGCAACGA AACAAAAGCA 
GATGATTGTA GAATTATCTG CTACCAAAGT CTTCCACTCA ATAATAAAGG CTCTCGCAAG 
TGCAGTCTAA ACTGTTTTCC TCTATGTAAA ATATATGAAG ACTATAAAGG AAAGCTCGAG 
GAGCTTAAAA TTATTAAAGG AAGTATGGAA CTAATCGACT TCAATGAAGA GGAACAACGT 
GAAATGAGCA GCATCAACGG AACTATGCAA GTATACGCTA TAATACCGAA AGAGAATGAA 
AAAGATATAT TGGATGAAAC GCAAGAAACA TCAAATCTAA ATAATGATTA CGAGGCTTTA 
AATCAAGAAA TTATGTCTTT AAGCCAAGAG ACTATACCTT CGAGTCAGGA AACTATTTCG 
GATGATACTG ACCTCGAATG CAATTGGATA GTAGAAAAAG CAAAACCGCA AACGCAGGAA 
CAACCAATTT TGGAAGGAAT AGATTTTGTC GATTTTAAAT ATGTTTTTAA TCAATTATTA 
ACACTCGACG ATCATTCCAA CATCGGATGC AGCATTCGAC ATTTTAAAAT AATTGGACGT 
TACCGGAATG GACTGAAAAT AACATATTCT GTAAAATGTG AAATGTGCAA TCAGATAATA 
CATATACCAT GCATTCAACA GAATAATGAC GTTATAGATA TCAATGAGAG CGCTGTTTCC 
GCGACAATGG TATTAGGCGG CGGATATGTT CAACTGATGG AGATGTTGGC TTGTCTGAAT 
GTGTCGTGTA TGTCCAAACA ATCATACGAC AAACACAGAA CAAAAATAAT AGAGGCCTTC 
GAACTGGCTG CACAGTGGGA AATGGAAGCT GCTGGTAAAA AAGAGAGAGA ACTAGCTATA 
GCAAGAGGTG ATGTATGTCC AGACTCTGGC ATACCATGGA TTCCAGTTGT CGCTGATGGT 
AGCTGGATGA AAAGATCATA CAAAGGTGGT TATTATAATT CTCCTTCCGG TGTAGGAGTT 
ATTGTTGGAT ATTATACAAA GAAAGTGTTA TATGTTGGCG TAAAGAATAA AGTCTGCATA 
ACTTGCCGGG TAGCGGCTAA GAAAAAAGAA AAACCTAGCG AGCATCGTTG CTTCAAAAAT 
TGGGGTACAA CTCAGAGTTC AACTGCAATG GAAAGCGTTG CTATTTTGGA GGGATTTGAA 
TGCAGCATAA AAATGCACGG CCTGATATAT TCAATATTAA TTGCCGATGG TGATAGCAGT 
GTCTACAAAA AGATATTGGA CCGTGATCCG TATAAACAGT ACTCGGTGCG TGTACAAAAA 
ATTGAATGTA TCAATCACAT GCTACGCAAT TTCGGTAAGA AAATTGCTAA TATCGGCACT 
ACAGGTGGGT GCCAAAAATT AAGAGCTGTC GTAAAAAAAA ACGCTCTCCG TCTGCGCGCT 
TGTATAAGGA AAGCTGCACA ATACCGCTAT AACGAAAACC TGCCACTGGT TGAAAAACTT 
GAAAGACTAA AAGCAGATTT AACTAACGTG CCTAGCCACG TTTTCGGAGA ACACAAGGAC 
TGTGAAAGAT TGGCATATTT CTGCAACCAG TACTCTGCTC CCAACGTAAT AAACTATGTT 
CCGCAACTGA AGGAAGCAGG TATTTATAAA CGTATAGAGG AAGCCATGCA GCCGCTTTTC 
AAGAATATTC AAAGTTTGCT GTACTGTATG AATAATAATG CAGCAGAAGT CTTTAATGCC 
ATCATCGCGA AATACATTGG TGGCAAACGA ATAAACTTTG GATTAAAAGG CTCGTATCAG 
GGTAGGGTTT ACGCTGCTGT CGTTCAGTAC AATACAAAAG AGGCAGTGAG CCGAATGTGT 
AGAGCAATAA ATAAGGAACC GCCAGTCCTT GTCAGGAAGA TTGAAGAACG ACGTAAAAGC 
GAGAATAGAA GATATTATAT ATCTTCCAGG CGGCGTAAAA GACAAATGTG TGATAAGGAT 
TACGGTCCTT GTGCGGAACA ACCTGAGATA GAAGAAATAG TTTTCACTCC CGAGTATGAG 
AAAAAAATAG CAGCACTCCA CAAATGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012145670.1
CDS	482..2731
Translation	MEINGFDGLEPWKFKDSRIRKLRNQKAASMSNDDQNNEANQQTDQTKVGEIKADGEHNEIKTNAECNEIKTDAECNETKADDCRIICYQSLPLNNKGSRKCSLNCFPLCKIYEDYKGKLEELKIIKGSMELIDFNEEEQREMSSINGTMQVYAIIPKENEKDILDETQETSNLNNDYEALNQEIMSLSQETIPSSQETISDDTDLECNWIVEKAKPQTQEQPILEGIDFVDFKYVFNQLLTLDDHSNIGCSIRHFKIIGRYRNGLKITYSVKCEMCNQIIHIPCIQQNNDVIDINESAVSATMVLGGGYVQLMEMLACLNVSCMSKQSYDKHRTKIIEAFELAAQWEMEAAGKKERELAIARGDVCPDSGIPWIPVVADGSWMKRSYKGGYYNSPSGVGVIVGYYTKKVLYVGVKNKVCITCRVAAKKKEKPSEHRCFKNWGTTQSSTAMESVAILEGFECSIKMHGLIYSILIADGDSSVYKKILDRDPYKQYSVRVQKIECINHMLRNFGKKIANIGTTGGCQKLRAVVKKNALRLRACIRKAAQYRYNENLPLVEKLERLKADLTNVPSHVFGEHKDCERLAYFCNQYSAPNVINYVPQLKEAGIYKRIEEAMQPLFKNIQSLLYCMNNNAAEVFNAIIAKYIGGKRINFGLKGSYQGRVYAAVVQYNTKEAVSRMCRAINKEPPVLVRKIEERRKSENRRYYISSRRRKRQMCDKDYGPCAEQPEIEEIVFTPEYEKKIAALHKC

Target ORF information:

RefSeq Version	XM_012290280.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata uncharacterized LOC105663106 (LOC105663106), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012290280.1

ORF Insert Sequence:

ATGGAAATCA ACGGATTTGA CGGTCTAGAA CCTTGGAAAT TCAAGGATTC GAGAATACGT 
AAGTTAAGAA ACCAAAAAGC GGCCTCTATG TCAAACGACG ATCAAAATAA TGAAGCAAAT 
CAACAAACTG ACCAAACTAA AGTTGGCGAA ATAAAAGCAG ATGGTGAACA CAACGAAATA 
AAAACAAATG CCGAATGCAA CGAAATAAAA ACAGATGCTG AATGCAACGA AACAAAAGCA 
GATGATTGTA GAATTATCTG CTACCAAAGT CTTCCACTCA ATAATAAAGG CTCTCGCAAG 
TGCAGTCTAA ACTGTTTTCC TCTATGTAAA ATATATGAAG ACTATAAAGG AAAGCTCGAG 
GAGCTTAAAA TTATTAAAGG AAGTATGGAA CTAATCGACT TCAATGAAGA GGAACAACGT 
GAAATGAGCA GCATCAACGG AACTATGCAA GTATACGCTA TAATACCGAA AGAGAATGAA 
AAAGATATAT TGGATGAAAC GCAAGAAACA TCAAATCTAA ATAATGATTA CGAGGCTTTA 
AATCAAGAAA TTATGTCTTT AAGCCAAGAG ACTATACCTT CGAGTCAGGA AACTATTTCG 
GATGATACTG ACCTCGAATG CAATTGGATA GTAGAAAAAG CAAAACCGCA AACGCAGGAA 
CAACCAATTT TGGAAGGAAT AGATTTTGTC GATTTTAAAT ATGTTTTTAA TCAATTATTA 
ACACTCGACG ATCATTCCAA CATCGGATGC AGCATTCGAC ATTTTAAAAT AATTGGACGT 
TACCGGAATG GACTGAAAAT AACATATTCT GTAAAATGTG AAATGTGCAA TCAGATAATA 
CATATACCAT GCATTCAACA GAATAATGAC GTTATAGATA TCAATGAGAG CGCTGTTTCC 
GCGACAATGG TATTAGGCGG CGGATATGTT CAACTGATGG AGATGTTGGC TTGTCTGAAT 
GTGTCGTGTA TGTCCAAACA ATCATACGAC AAACACAGAA CAAAAATAAT AGAGGCCTTC 
GAACTGGCTG CACAGTGGGA AATGGAAGCT GCTGGTAAAA AAGAGAGAGA ACTAGCTATA 
GCAAGAGGTG ATGTATGTCC AGACTCTGGC ATACCATGGA TTCCAGTTGT CGCTGATGGT 
AGCTGGATGA AAAGATCATA CAAAGGTGGT TATTATAATT CTCCTTCCGG TGTAGGAGTT 
ATTGTTGGAT ATTATACAAA GAAAGTGTTA TATGTTGGCG TAAAGAATAA AGTCTGCATA 
ACTTGCCGGG TAGCGGCTAA GAAAAAAGAA AAACCTAGCG AGCATCGTTG CTTCAAAAAT 
TGGGGTACAA CTCAGAGTTC AACTGCAATG GAAAGCGTTG CTATTTTGGA GGGATTTGAA 
TGCAGCATAA AAATGCACGG CCTGATATAT TCAATATTAA TTGCCGATGG TGATAGCAGT 
GTCTACAAAA AGATATTGGA CCGTGATCCG TATAAACAGT ACTCGGTGCG TGTACAAAAA 
ATTGAATGTA TCAATCACAT GCTACGCAAT TTCGGTAAGA AAATTGCTAA TATCGGCACT 
ACAGGTGGGT GCCAAAAATT AAGAGCTGTC GTAAAAAAAA ACGCTCTCCG TCTGCGCGCT 
TGTATAAGGA AAGCTGCACA ATACCGCTAT AACGAAAACC TGCCACTGGT TGAAAAACTT 
GAAAGACTAA AAGCAGATTT AACTAACGTG CCTAGCCACG TTTTCGGAGA ACACAAGGAC 
TGTGAAAGAT TGGCATATTT CTGCAACCAG TACTCTGCTC CCAACGTAAT AAACTATGTT 
CCGCAACTGA AGGAAGCAGG TATTTATAAA CGTATAGAGG AAGCCATGCA GCCGCTTTTC 
AAGAATATTC AAAGTTTGCT GTACTGTATG AATAATAATG CAGCAGAAGT CTTTAATGCC 
ATCATCGCGA AATACATTGG TGGCAAACGA ATAAACTTTG GATTAAAAGG CTCGTATCAG 
GGTAGGGTTT ACGCTGCTGT CGTTCAGTAC AATACAAAAG AGGCAGTGAG CCGAATGTGT 
AGAGCAATAA ATAAGGAACC GCCAGTCCTT GTCAGGAAGA TTGAAGAACG ACGTAAAAGC 
GAGAATAGAA GATATTATAT ATCTTCCAGG CGGCGTAAAA GACAAATGTG TGATAAGGAT 
TACGGTCCTT GTGCGGAACA ACCTGAGATA GAAGAAATAG TTTTCACTCC CGAGTATGAG 
AAAAAAATAG CAGCACTCCA CAAATGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105663106 ( XM_012290281.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012145671.1 Megachile rotundata uncharacterized LOC105663106 (LOC105663106), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OMe108974
Clone ID Related Accession (Same CDS sequence)	XM_012290281.1
Accession Version	XM_012290281.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	uncharacterized protein LOC105663106 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797277.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTTAT TGCCTTCGGT TTTGAGTCAT TTCTTGCTAC GTAAGTTAAG AAACCAAAAA 
GCGGCCTCTA TGTCAAACGA CGATCAAAAT AATGAAGCAA ATCAACAAAC TGACCAAACT 
AAAGTTGGCG AAATAAAAGC AGATGGTGAA CACAACGAAA TAAAAACAAA TGCCGAATGC 
AACGAAATAA AAACAGATGC TGAATGCAAC GAAACAAAAG CAGATGATTG TAGAATTATC 
TGCTACCAAA GTCTTCCACT CAATAATAAA GGCTCTCGCA AGTGCAGTCT AAACTGTTTT 
CCTCTATGTA AAATATATGA AGACTATAAA GGAAAGCTCG AGGAGCTTAA AATTATTAAA 
GGAAGTATGG AACTAATCGA CTTCAATGAA GAGGAACAAC GTGAAATGAG CAGCATCAAC 
GGAACTATGC AAGTATACGC TATAATACCG AAAGAGAATG AAAAAGATAT ATTGGATGAA 
ACGCAAGAAA CATCAAATCT AAATAATGAT TACGAGGCTT TAAATCAAGA AATTATGTCT 
TTAAGCCAAG AGACTATACC TTCGAGTCAG GAAACTATTT CGGATGATAC TGACCTCGAA 
TGCAATTGGA TAGTAGAAAA AGCAAAACCG CAAACGCAGG AACAACCAAT TTTGGAAGGA 
ATAGATTTTG TCGATTTTAA ATATGTTTTT AATCAATTAT TAACACTCGA CGATCATTCC 
AACATCGGAT GCAGCATTCG ACATTTTAAA ATAATTGGAC GTTACCGGAA TGGACTGAAA 
ATAACATATT CTGTAAAATG TGAAATGTGC AATCAGATAA TACATATACC ATGCATTCAA 
CAGAATAATG ACGTTATAGA TATCAATGAG AGCGCTGTTT CCGCGACAAT GGTATTAGGC 
GGCGGATATG TTCAACTGAT GGAGATGTTG GCTTGTCTGA ATGTGTCGTG TATGTCCAAA 
CAATCATACG ACAAACACAG AACAAAAATA ATAGAGGCCT TCGAACTGGC TGCACAGTGG 
GAAATGGAAG CTGCTGGTAA AAAAGAGAGA GAACTAGCTA TAGCAAGAGG TGATGTATGT 
CCAGACTCTG GCATACCATG GATTCCAGTT GTCGCTGATG GTAGCTGGAT GAAAAGATCA 
TACAAAGGTG GTTATTATAA TTCTCCTTCC GGTGTAGGAG TTATTGTTGG ATATTATACA 
AAGAAAGTGT TATATGTTGG CGTAAAGAAT AAAGTCTGCA TAACTTGCCG GGTAGCGGCT 
AAGAAAAAAG AAAAACCTAG CGAGCATCGT TGCTTCAAAA ATTGGGGTAC AACTCAGAGT 
TCAACTGCAA TGGAAAGCGT TGCTATTTTG GAGGGATTTG AATGCAGCAT AAAAATGCAC 
GGCCTGATAT ATTCAATATT AATTGCCGAT GGTGATAGCA GTGTCTACAA AAAGATATTG 
GACCGTGATC CGTATAAACA GTACTCGGTG CGTGTACAAA AAATTGAATG TATCAATCAC 
ATGCTACGCA ATTTCGGTAA GAAAATTGCT AATATCGGCA CTACAGGTGG GTGCCAAAAA 
TTAAGAGCTG TCGTAAAAAA AAACGCTCTC CGTCTGCGCG CTTGTATAAG GAAAGCTGCA 
CAATACCGCT ATAACGAAAA CCTGCCACTG GTTGAAAAAC TTGAAAGACT AAAAGCAGAT 
TTAACTAACG TGCCTAGCCA CGTTTTCGGA GAACACAAGG ACTGTGAAAG ATTGGCATAT 
TTCTGCAACC AGTACTCTGC TCCCAACGTA ATAAACTATG TTCCGCAACT GAAGGAAGCA 
GGTATTTATA AACGTATAGA GGAAGCCATG CAGCCGCTTT TCAAGAATAT TCAAAGTTTG 
CTGTACTGTA TGAATAATAA TGCAGCAGAA GTCTTTAATG CCATCATCGC GAAATACATT 
GGTGGCAAAC GAATAAACTT TGGATTAAAA GGCTCGTATC AGGGTAGGGT TTACGCTGCT 
GTCGTTCAGT ACAATACAAA AGAGGCAGTG AGCCGAATGT GTAGAGCAAT AAATAAGGAA 
CCGCCAGTCC TTGTCAGGAA GATTGAAGAA CGACGTAAAA GCGAGAATAG AAGATATTAT 
ATATCTTCCA GGCGGCGTAA AAGACAAATG TGTGATAAGG ATTACGGTCC TTGTGCGGAA 
CAACCTGAGA TAGAAGAAAT AGTTTTCACT CCCGAGTATG AGAAAAAAAT AGCAGCACTC 
CACAAATGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012145671.1
CDS	1003..3234
Translation	MMLLPSVLSHFLLRKLRNQKAASMSNDDQNNEANQQTDQTKVGEIKADGEHNEIKTNAECNEIKTDAECNETKADDCRIICYQSLPLNNKGSRKCSLNCFPLCKIYEDYKGKLEELKIIKGSMELIDFNEEEQREMSSINGTMQVYAIIPKENEKDILDETQETSNLNNDYEALNQEIMSLSQETIPSSQETISDDTDLECNWIVEKAKPQTQEQPILEGIDFVDFKYVFNQLLTLDDHSNIGCSIRHFKIIGRYRNGLKITYSVKCEMCNQIIHIPCIQQNNDVIDINESAVSATMVLGGGYVQLMEMLACLNVSCMSKQSYDKHRTKIIEAFELAAQWEMEAAGKKERELAIARGDVCPDSGIPWIPVVADGSWMKRSYKGGYYNSPSGVGVIVGYYTKKVLYVGVKNKVCITCRVAAKKKEKPSEHRCFKNWGTTQSSTAMESVAILEGFECSIKMHGLIYSILIADGDSSVYKKILDRDPYKQYSVRVQKIECINHMLRNFGKKIANIGTTGGCQKLRAVVKKNALRLRACIRKAAQYRYNENLPLVEKLERLKADLTNVPSHVFGEHKDCERLAYFCNQYSAPNVINYVPQLKEAGIYKRIEEAMQPLFKNIQSLLYCMNNNAAEVFNAIIAKYIGGKRINFGLKGSYQGRVYAAVVQYNTKEAVSRMCRAINKEPPVLVRKIEERRKSENRRYYISSRRRKRQMCDKDYGPCAEQPEIEEIVFTPEYEKKIAALHKC

Target ORF information:

RefSeq Version	XM_012290281.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata uncharacterized LOC105663106 (LOC105663106), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012290281.1

ORF Insert Sequence:

ATGATGTTAT TGCCTTCGGT TTTGAGTCAT TTCTTGCTAC GTAAGTTAAG AAACCAAAAA 
GCGGCCTCTA TGTCAAACGA CGATCAAAAT AATGAAGCAA ATCAACAAAC TGACCAAACT 
AAAGTTGGCG AAATAAAAGC AGATGGTGAA CACAACGAAA TAAAAACAAA TGCCGAATGC 
AACGAAATAA AAACAGATGC TGAATGCAAC GAAACAAAAG CAGATGATTG TAGAATTATC 
TGCTACCAAA GTCTTCCACT CAATAATAAA GGCTCTCGCA AGTGCAGTCT AAACTGTTTT 
CCTCTATGTA AAATATATGA AGACTATAAA GGAAAGCTCG AGGAGCTTAA AATTATTAAA 
GGAAGTATGG AACTAATCGA CTTCAATGAA GAGGAACAAC GTGAAATGAG CAGCATCAAC 
GGAACTATGC AAGTATACGC TATAATACCG AAAGAGAATG AAAAAGATAT ATTGGATGAA 
ACGCAAGAAA CATCAAATCT AAATAATGAT TACGAGGCTT TAAATCAAGA AATTATGTCT 
TTAAGCCAAG AGACTATACC TTCGAGTCAG GAAACTATTT CGGATGATAC TGACCTCGAA 
TGCAATTGGA TAGTAGAAAA AGCAAAACCG CAAACGCAGG AACAACCAAT TTTGGAAGGA 
ATAGATTTTG TCGATTTTAA ATATGTTTTT AATCAATTAT TAACACTCGA CGATCATTCC 
AACATCGGAT GCAGCATTCG ACATTTTAAA ATAATTGGAC GTTACCGGAA TGGACTGAAA 
ATAACATATT CTGTAAAATG TGAAATGTGC AATCAGATAA TACATATACC ATGCATTCAA 
CAGAATAATG ACGTTATAGA TATCAATGAG AGCGCTGTTT CCGCGACAAT GGTATTAGGC 
GGCGGATATG TTCAACTGAT GGAGATGTTG GCTTGTCTGA ATGTGTCGTG TATGTCCAAA 
CAATCATACG ACAAACACAG AACAAAAATA ATAGAGGCCT TCGAACTGGC TGCACAGTGG 
GAAATGGAAG CTGCTGGTAA AAAAGAGAGA GAACTAGCTA TAGCAAGAGG TGATGTATGT 
CCAGACTCTG GCATACCATG GATTCCAGTT GTCGCTGATG GTAGCTGGAT GAAAAGATCA 
TACAAAGGTG GTTATTATAA TTCTCCTTCC GGTGTAGGAG TTATTGTTGG ATATTATACA 
AAGAAAGTGT TATATGTTGG CGTAAAGAAT AAAGTCTGCA TAACTTGCCG GGTAGCGGCT 
AAGAAAAAAG AAAAACCTAG CGAGCATCGT TGCTTCAAAA ATTGGGGTAC AACTCAGAGT 
TCAACTGCAA TGGAAAGCGT TGCTATTTTG GAGGGATTTG AATGCAGCAT AAAAATGCAC 
GGCCTGATAT ATTCAATATT AATTGCCGAT GGTGATAGCA GTGTCTACAA AAAGATATTG 
GACCGTGATC CGTATAAACA GTACTCGGTG CGTGTACAAA AAATTGAATG TATCAATCAC 
ATGCTACGCA ATTTCGGTAA GAAAATTGCT AATATCGGCA CTACAGGTGG GTGCCAAAAA 
TTAAGAGCTG TCGTAAAAAA AAACGCTCTC CGTCTGCGCG CTTGTATAAG GAAAGCTGCA 
CAATACCGCT ATAACGAAAA CCTGCCACTG GTTGAAAAAC TTGAAAGACT AAAAGCAGAT 
TTAACTAACG TGCCTAGCCA CGTTTTCGGA GAACACAAGG ACTGTGAAAG ATTGGCATAT 
TTCTGCAACC AGTACTCTGC TCCCAACGTA ATAAACTATG TTCCGCAACT GAAGGAAGCA 
GGTATTTATA AACGTATAGA GGAAGCCATG CAGCCGCTTT TCAAGAATAT TCAAAGTTTG 
CTGTACTGTA TGAATAATAA TGCAGCAGAA GTCTTTAATG CCATCATCGC GAAATACATT 
GGTGGCAAAC GAATAAACTT TGGATTAAAA GGCTCGTATC AGGGTAGGGT TTACGCTGCT 
GTCGTTCAGT ACAATACAAA AGAGGCAGTG AGCCGAATGT GTAGAGCAAT AAATAAGGAA 
CCGCCAGTCC TTGTCAGGAA GATTGAAGAA CGACGTAAAA GCGAGAATAG AAGATATTAT 
ATATCTTCCA GGCGGCGTAA AAGACAAATG TGTGATAAGG ATTACGGTCC TTGTGCGGAA 
CAACCTGAGA TAGAAGAAAT AGTTTTCACT CCCGAGTATG AGAAAAAAAT AGCAGCACTC 
CACAAATGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105663106 ( XM_012290283.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012145673.1 Megachile rotundata uncharacterized LOC105663106 (LOC105663106), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OMe108975
Clone ID Related Accession (Same CDS sequence)	XM_012290283.1 , XM_012290284.1 , XM_012290282.1 , XM_012290285.1
Accession Version	XM_012290283.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	uncharacterized protein LOC105663106 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797277.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAACG ACGATCAAAA TAATGAAGCA AATCAACAAA CTGACCAAAC TAAAGTTGGC 
GAAATAAAAG CAGATGGTGA ACACAACGAA ATAAAAACAA ATGCCGAATG CAACGAAATA 
AAAACAGATG CTGAATGCAA CGAAACAAAA GCAGATGATT GTAGAATTAT CTGCTACCAA 
AGTCTTCCAC TCAATAATAA AGGCTCTCGC AAGTGCAGTC TAAACTGTTT TCCTCTATGT 
AAAATATATG AAGACTATAA AGGAAAGCTC GAGGAGCTTA AAATTATTAA AGGAAGTATG 
GAACTAATCG ACTTCAATGA AGAGGAACAA CGTGAAATGA GCAGCATCAA CGGAACTATG 
CAAGTATACG CTATAATACC GAAAGAGAAT GAAAAAGATA TATTGGATGA AACGCAAGAA 
ACATCAAATC TAAATAATGA TTACGAGGCT TTAAATCAAG AAATTATGTC TTTAAGCCAA 
GAGACTATAC CTTCGAGTCA GGAAACTATT TCGGATGATA CTGACCTCGA ATGCAATTGG 
ATAGTAGAAA AAGCAAAACC GCAAACGCAG GAACAACCAA TTTTGGAAGG AATAGATTTT 
GTCGATTTTA AATATGTTTT TAATCAATTA TTAACACTCG ACGATCATTC CAACATCGGA 
TGCAGCATTC GACATTTTAA AATAATTGGA CGTTACCGGA ATGGACTGAA AATAACATAT 
TCTGTAAAAT GTGAAATGTG CAATCAGATA ATACATATAC CATGCATTCA ACAGAATAAT 
GACGTTATAG ATATCAATGA GAGCGCTGTT TCCGCGACAA TGGTATTAGG CGGCGGATAT 
GTTCAACTGA TGGAGATGTT GGCTTGTCTG AATGTGTCGT GTATGTCCAA ACAATCATAC 
GACAAACACA GAACAAAAAT AATAGAGGCC TTCGAACTGG CTGCACAGTG GGAAATGGAA 
GCTGCTGGTA AAAAAGAGAG AGAACTAGCT ATAGCAAGAG GTGATGTATG TCCAGACTCT 
GGCATACCAT GGATTCCAGT TGTCGCTGAT GGTAGCTGGA TGAAAAGATC ATACAAAGGT 
GGTTATTATA ATTCTCCTTC CGGTGTAGGA GTTATTGTTG GATATTATAC AAAGAAAGTG 
TTATATGTTG GCGTAAAGAA TAAAGTCTGC ATAACTTGCC GGGTAGCGGC TAAGAAAAAA 
GAAAAACCTA GCGAGCATCG TTGCTTCAAA AATTGGGGTA CAACTCAGAG TTCAACTGCA 
ATGGAAAGCG TTGCTATTTT GGAGGGATTT GAATGCAGCA TAAAAATGCA CGGCCTGATA 
TATTCAATAT TAATTGCCGA TGGTGATAGC AGTGTCTACA AAAAGATATT GGACCGTGAT 
CCGTATAAAC AGTACTCGGT GCGTGTACAA AAAATTGAAT GTATCAATCA CATGCTACGC 
AATTTCGGTA AGAAAATTGC TAATATCGGC ACTACAGGTG GGTGCCAAAA ATTAAGAGCT 
GTCGTAAAAA AAAACGCTCT CCGTCTGCGC GCTTGTATAA GGAAAGCTGC ACAATACCGC 
TATAACGAAA ACCTGCCACT GGTTGAAAAA CTTGAAAGAC TAAAAGCAGA TTTAACTAAC 
GTGCCTAGCC ACGTTTTCGG AGAACACAAG GACTGTGAAA GATTGGCATA TTTCTGCAAC 
CAGTACTCTG CTCCCAACGT AATAAACTAT GTTCCGCAAC TGAAGGAAGC AGGTATTTAT 
AAACGTATAG AGGAAGCCAT GCAGCCGCTT TTCAAGAATA TTCAAAGTTT GCTGTACTGT 
ATGAATAATA ATGCAGCAGA AGTCTTTAAT GCCATCATCG CGAAATACAT TGGTGGCAAA 
CGAATAAACT TTGGATTAAA AGGCTCGTAT CAGGGTAGGG TTTACGCTGC TGTCGTTCAG 
TACAATACAA AAGAGGCAGT GAGCCGAATG TGTAGAGCAA TAAATAAGGA ACCGCCAGTC 
CTTGTCAGGA AGATTGAAGA ACGACGTAAA AGCGAGAATA GAAGATATTA TATATCTTCC 
AGGCGGCGTA AAAGACAAAT GTGTGATAAG GATTACGGTC CTTGTGCGGA ACAACCTGAG 
ATAGAAGAAA TAGTTTTCAC TCCCGAGTAT GAGAAAAAAA TAGCAGCACT CCACAAATGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012145673.1
CDS	308..2470
Translation	MSNDDQNNEANQQTDQTKVGEIKADGEHNEIKTNAECNEIKTDAECNETKADDCRIICYQSLPLNNKGSRKCSLNCFPLCKIYEDYKGKLEELKIIKGSMELIDFNEEEQREMSSINGTMQVYAIIPKENEKDILDETQETSNLNNDYEALNQEIMSLSQETIPSSQETISDDTDLECNWIVEKAKPQTQEQPILEGIDFVDFKYVFNQLLTLDDHSNIGCSIRHFKIIGRYRNGLKITYSVKCEMCNQIIHIPCIQQNNDVIDINESAVSATMVLGGGYVQLMEMLACLNVSCMSKQSYDKHRTKIIEAFELAAQWEMEAAGKKERELAIARGDVCPDSGIPWIPVVADGSWMKRSYKGGYYNSPSGVGVIVGYYTKKVLYVGVKNKVCITCRVAAKKKEKPSEHRCFKNWGTTQSSTAMESVAILEGFECSIKMHGLIYSILIADGDSSVYKKILDRDPYKQYSVRVQKIECINHMLRNFGKKIANIGTTGGCQKLRAVVKKNALRLRACIRKAAQYRYNENLPLVEKLERLKADLTNVPSHVFGEHKDCERLAYFCNQYSAPNVINYVPQLKEAGIYKRIEEAMQPLFKNIQSLLYCMNNNAAEVFNAIIAKYIGGKRINFGLKGSYQGRVYAAVVQYNTKEAVSRMCRAINKEPPVLVRKIEERRKSENRRYYISSRRRKRQMCDKDYGPCAEQPEIEEIVFTPEYEKKIAALHKC

Target ORF information:

RefSeq Version	XM_012290283.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata uncharacterized LOC105663106 (LOC105663106), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012290283.1

ORF Insert Sequence:

ATGTCAAACG ACGATCAAAA TAATGAAGCA AATCAACAAA CTGACCAAAC TAAAGTTGGC 
GAAATAAAAG CAGATGGTGA ACACAACGAA ATAAAAACAA ATGCCGAATG CAACGAAATA 
AAAACAGATG CTGAATGCAA CGAAACAAAA GCAGATGATT GTAGAATTAT CTGCTACCAA 
AGTCTTCCAC TCAATAATAA AGGCTCTCGC AAGTGCAGTC TAAACTGTTT TCCTCTATGT 
AAAATATATG AAGACTATAA AGGAAAGCTC GAGGAGCTTA AAATTATTAA AGGAAGTATG 
GAACTAATCG ACTTCAATGA AGAGGAACAA CGTGAAATGA GCAGCATCAA CGGAACTATG 
CAAGTATACG CTATAATACC GAAAGAGAAT GAAAAAGATA TATTGGATGA AACGCAAGAA 
ACATCAAATC TAAATAATGA TTACGAGGCT TTAAATCAAG AAATTATGTC TTTAAGCCAA 
GAGACTATAC CTTCGAGTCA GGAAACTATT TCGGATGATA CTGACCTCGA ATGCAATTGG 
ATAGTAGAAA AAGCAAAACC GCAAACGCAG GAACAACCAA TTTTGGAAGG AATAGATTTT 
GTCGATTTTA AATATGTTTT TAATCAATTA TTAACACTCG ACGATCATTC CAACATCGGA 
TGCAGCATTC GACATTTTAA AATAATTGGA CGTTACCGGA ATGGACTGAA AATAACATAT 
TCTGTAAAAT GTGAAATGTG CAATCAGATA ATACATATAC CATGCATTCA ACAGAATAAT 
GACGTTATAG ATATCAATGA GAGCGCTGTT TCCGCGACAA TGGTATTAGG CGGCGGATAT 
GTTCAACTGA TGGAGATGTT GGCTTGTCTG AATGTGTCGT GTATGTCCAA ACAATCATAC 
GACAAACACA GAACAAAAAT AATAGAGGCC TTCGAACTGG CTGCACAGTG GGAAATGGAA 
GCTGCTGGTA AAAAAGAGAG AGAACTAGCT ATAGCAAGAG GTGATGTATG TCCAGACTCT 
GGCATACCAT GGATTCCAGT TGTCGCTGAT GGTAGCTGGA TGAAAAGATC ATACAAAGGT 
GGTTATTATA ATTCTCCTTC CGGTGTAGGA GTTATTGTTG GATATTATAC AAAGAAAGTG 
TTATATGTTG GCGTAAAGAA TAAAGTCTGC ATAACTTGCC GGGTAGCGGC TAAGAAAAAA 
GAAAAACCTA GCGAGCATCG TTGCTTCAAA AATTGGGGTA CAACTCAGAG TTCAACTGCA 
ATGGAAAGCG TTGCTATTTT GGAGGGATTT GAATGCAGCA TAAAAATGCA CGGCCTGATA 
TATTCAATAT TAATTGCCGA TGGTGATAGC AGTGTCTACA AAAAGATATT GGACCGTGAT 
CCGTATAAAC AGTACTCGGT GCGTGTACAA AAAATTGAAT GTATCAATCA CATGCTACGC 
AATTTCGGTA AGAAAATTGC TAATATCGGC ACTACAGGTG GGTGCCAAAA ATTAAGAGCT 
GTCGTAAAAA AAAACGCTCT CCGTCTGCGC GCTTGTATAA GGAAAGCTGC ACAATACCGC 
TATAACGAAA ACCTGCCACT GGTTGAAAAA CTTGAAAGAC TAAAAGCAGA TTTAACTAAC 
GTGCCTAGCC ACGTTTTCGG AGAACACAAG GACTGTGAAA GATTGGCATA TTTCTGCAAC 
CAGTACTCTG CTCCCAACGT AATAAACTAT GTTCCGCAAC TGAAGGAAGC AGGTATTTAT 
AAACGTATAG AGGAAGCCAT GCAGCCGCTT TTCAAGAATA TTCAAAGTTT GCTGTACTGT 
ATGAATAATA ATGCAGCAGA AGTCTTTAAT GCCATCATCG CGAAATACAT TGGTGGCAAA 
CGAATAAACT TTGGATTAAA AGGCTCGTAT CAGGGTAGGG TTTACGCTGC TGTCGTTCAG 
TACAATACAA AAGAGGCAGT GAGCCGAATG TGTAGAGCAA TAAATAAGGA ACCGCCAGTC 
CTTGTCAGGA AGATTGAAGA ACGACGTAAA AGCGAGAATA GAAGATATTA TATATCTTCC 
AGGCGGCGTA AAAGACAAAT GTGTGATAAG GATTACGGTC CTTGTGCGGA ACAACCTGAG 
ATAGAAGAAA TAGTTTTCAC TCCCGAGTAT GAGAAAAAAA TAGCAGCACT CCACAAATGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105663106 ( XM_012290284.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012145674.1 Megachile rotundata uncharacterized LOC105663106 (LOC105663106), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OMe108975
Clone ID Related Accession (Same CDS sequence)	XM_012290283.1 , XM_012290284.1 , XM_012290282.1 , XM_012290285.1
Accession Version	XM_012290284.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	uncharacterized protein LOC105663106 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797277.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAACG ACGATCAAAA TAATGAAGCA AATCAACAAA CTGACCAAAC TAAAGTTGGC 
GAAATAAAAG CAGATGGTGA ACACAACGAA ATAAAAACAA ATGCCGAATG CAACGAAATA 
AAAACAGATG CTGAATGCAA CGAAACAAAA GCAGATGATT GTAGAATTAT CTGCTACCAA 
AGTCTTCCAC TCAATAATAA AGGCTCTCGC AAGTGCAGTC TAAACTGTTT TCCTCTATGT 
AAAATATATG AAGACTATAA AGGAAAGCTC GAGGAGCTTA AAATTATTAA AGGAAGTATG 
GAACTAATCG ACTTCAATGA AGAGGAACAA CGTGAAATGA GCAGCATCAA CGGAACTATG 
CAAGTATACG CTATAATACC GAAAGAGAAT GAAAAAGATA TATTGGATGA AACGCAAGAA 
ACATCAAATC TAAATAATGA TTACGAGGCT TTAAATCAAG AAATTATGTC TTTAAGCCAA 
GAGACTATAC CTTCGAGTCA GGAAACTATT TCGGATGATA CTGACCTCGA ATGCAATTGG 
ATAGTAGAAA AAGCAAAACC GCAAACGCAG GAACAACCAA TTTTGGAAGG AATAGATTTT 
GTCGATTTTA AATATGTTTT TAATCAATTA TTAACACTCG ACGATCATTC CAACATCGGA 
TGCAGCATTC GACATTTTAA AATAATTGGA CGTTACCGGA ATGGACTGAA AATAACATAT 
TCTGTAAAAT GTGAAATGTG CAATCAGATA ATACATATAC CATGCATTCA ACAGAATAAT 
GACGTTATAG ATATCAATGA GAGCGCTGTT TCCGCGACAA TGGTATTAGG CGGCGGATAT 
GTTCAACTGA TGGAGATGTT GGCTTGTCTG AATGTGTCGT GTATGTCCAA ACAATCATAC 
GACAAACACA GAACAAAAAT AATAGAGGCC TTCGAACTGG CTGCACAGTG GGAAATGGAA 
GCTGCTGGTA AAAAAGAGAG AGAACTAGCT ATAGCAAGAG GTGATGTATG TCCAGACTCT 
GGCATACCAT GGATTCCAGT TGTCGCTGAT GGTAGCTGGA TGAAAAGATC ATACAAAGGT 
GGTTATTATA ATTCTCCTTC CGGTGTAGGA GTTATTGTTG GATATTATAC AAAGAAAGTG 
TTATATGTTG GCGTAAAGAA TAAAGTCTGC ATAACTTGCC GGGTAGCGGC TAAGAAAAAA 
GAAAAACCTA GCGAGCATCG TTGCTTCAAA AATTGGGGTA CAACTCAGAG TTCAACTGCA 
ATGGAAAGCG TTGCTATTTT GGAGGGATTT GAATGCAGCA TAAAAATGCA CGGCCTGATA 
TATTCAATAT TAATTGCCGA TGGTGATAGC AGTGTCTACA AAAAGATATT GGACCGTGAT 
CCGTATAAAC AGTACTCGGT GCGTGTACAA AAAATTGAAT GTATCAATCA CATGCTACGC 
AATTTCGGTA AGAAAATTGC TAATATCGGC ACTACAGGTG GGTGCCAAAA ATTAAGAGCT 
GTCGTAAAAA AAAACGCTCT CCGTCTGCGC GCTTGTATAA GGAAAGCTGC ACAATACCGC 
TATAACGAAA ACCTGCCACT GGTTGAAAAA CTTGAAAGAC TAAAAGCAGA TTTAACTAAC 
GTGCCTAGCC ACGTTTTCGG AGAACACAAG GACTGTGAAA GATTGGCATA TTTCTGCAAC 
CAGTACTCTG CTCCCAACGT AATAAACTAT GTTCCGCAAC TGAAGGAAGC AGGTATTTAT 
AAACGTATAG AGGAAGCCAT GCAGCCGCTT TTCAAGAATA TTCAAAGTTT GCTGTACTGT 
ATGAATAATA ATGCAGCAGA AGTCTTTAAT GCCATCATCG CGAAATACAT TGGTGGCAAA 
CGAATAAACT TTGGATTAAA AGGCTCGTAT CAGGGTAGGG TTTACGCTGC TGTCGTTCAG 
TACAATACAA AAGAGGCAGT GAGCCGAATG TGTAGAGCAA TAAATAAGGA ACCGCCAGTC 
CTTGTCAGGA AGATTGAAGA ACGACGTAAA AGCGAGAATA GAAGATATTA TATATCTTCC 
AGGCGGCGTA AAAGACAAAT GTGTGATAAG GATTACGGTC CTTGTGCGGA ACAACCTGAG 
ATAGAAGAAA TAGTTTTCAC TCCCGAGTAT GAGAAAAAAA TAGCAGCACT CCACAAATGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012145674.1
CDS	51..2213
Translation	MSNDDQNNEANQQTDQTKVGEIKADGEHNEIKTNAECNEIKTDAECNETKADDCRIICYQSLPLNNKGSRKCSLNCFPLCKIYEDYKGKLEELKIIKGSMELIDFNEEEQREMSSINGTMQVYAIIPKENEKDILDETQETSNLNNDYEALNQEIMSLSQETIPSSQETISDDTDLECNWIVEKAKPQTQEQPILEGIDFVDFKYVFNQLLTLDDHSNIGCSIRHFKIIGRYRNGLKITYSVKCEMCNQIIHIPCIQQNNDVIDINESAVSATMVLGGGYVQLMEMLACLNVSCMSKQSYDKHRTKIIEAFELAAQWEMEAAGKKERELAIARGDVCPDSGIPWIPVVADGSWMKRSYKGGYYNSPSGVGVIVGYYTKKVLYVGVKNKVCITCRVAAKKKEKPSEHRCFKNWGTTQSSTAMESVAILEGFECSIKMHGLIYSILIADGDSSVYKKILDRDPYKQYSVRVQKIECINHMLRNFGKKIANIGTTGGCQKLRAVVKKNALRLRACIRKAAQYRYNENLPLVEKLERLKADLTNVPSHVFGEHKDCERLAYFCNQYSAPNVINYVPQLKEAGIYKRIEEAMQPLFKNIQSLLYCMNNNAAEVFNAIIAKYIGGKRINFGLKGSYQGRVYAAVVQYNTKEAVSRMCRAINKEPPVLVRKIEERRKSENRRYYISSRRRKRQMCDKDYGPCAEQPEIEEIVFTPEYEKKIAALHKC

Target ORF information:

RefSeq Version	XM_012290284.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata uncharacterized LOC105663106 (LOC105663106), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012290284.1

ORF Insert Sequence:

ATGTCAAACG ACGATCAAAA TAATGAAGCA AATCAACAAA CTGACCAAAC TAAAGTTGGC 
GAAATAAAAG CAGATGGTGA ACACAACGAA ATAAAAACAA ATGCCGAATG CAACGAAATA 
AAAACAGATG CTGAATGCAA CGAAACAAAA GCAGATGATT GTAGAATTAT CTGCTACCAA 
AGTCTTCCAC TCAATAATAA AGGCTCTCGC AAGTGCAGTC TAAACTGTTT TCCTCTATGT 
AAAATATATG AAGACTATAA AGGAAAGCTC GAGGAGCTTA AAATTATTAA AGGAAGTATG 
GAACTAATCG ACTTCAATGA AGAGGAACAA CGTGAAATGA GCAGCATCAA CGGAACTATG 
CAAGTATACG CTATAATACC GAAAGAGAAT GAAAAAGATA TATTGGATGA AACGCAAGAA 
ACATCAAATC TAAATAATGA TTACGAGGCT TTAAATCAAG AAATTATGTC TTTAAGCCAA 
GAGACTATAC CTTCGAGTCA GGAAACTATT TCGGATGATA CTGACCTCGA ATGCAATTGG 
ATAGTAGAAA AAGCAAAACC GCAAACGCAG GAACAACCAA TTTTGGAAGG AATAGATTTT 
GTCGATTTTA AATATGTTTT TAATCAATTA TTAACACTCG ACGATCATTC CAACATCGGA 
TGCAGCATTC GACATTTTAA AATAATTGGA CGTTACCGGA ATGGACTGAA AATAACATAT 
TCTGTAAAAT GTGAAATGTG CAATCAGATA ATACATATAC CATGCATTCA ACAGAATAAT 
GACGTTATAG ATATCAATGA GAGCGCTGTT TCCGCGACAA TGGTATTAGG CGGCGGATAT 
GTTCAACTGA TGGAGATGTT GGCTTGTCTG AATGTGTCGT GTATGTCCAA ACAATCATAC 
GACAAACACA GAACAAAAAT AATAGAGGCC TTCGAACTGG CTGCACAGTG GGAAATGGAA 
GCTGCTGGTA AAAAAGAGAG AGAACTAGCT ATAGCAAGAG GTGATGTATG TCCAGACTCT 
GGCATACCAT GGATTCCAGT TGTCGCTGAT GGTAGCTGGA TGAAAAGATC ATACAAAGGT 
GGTTATTATA ATTCTCCTTC CGGTGTAGGA GTTATTGTTG GATATTATAC AAAGAAAGTG 
TTATATGTTG GCGTAAAGAA TAAAGTCTGC ATAACTTGCC GGGTAGCGGC TAAGAAAAAA 
GAAAAACCTA GCGAGCATCG TTGCTTCAAA AATTGGGGTA CAACTCAGAG TTCAACTGCA 
ATGGAAAGCG TTGCTATTTT GGAGGGATTT GAATGCAGCA TAAAAATGCA CGGCCTGATA 
TATTCAATAT TAATTGCCGA TGGTGATAGC AGTGTCTACA AAAAGATATT GGACCGTGAT 
CCGTATAAAC AGTACTCGGT GCGTGTACAA AAAATTGAAT GTATCAATCA CATGCTACGC 
AATTTCGGTA AGAAAATTGC TAATATCGGC ACTACAGGTG GGTGCCAAAA ATTAAGAGCT 
GTCGTAAAAA AAAACGCTCT CCGTCTGCGC GCTTGTATAA GGAAAGCTGC ACAATACCGC 
TATAACGAAA ACCTGCCACT GGTTGAAAAA CTTGAAAGAC TAAAAGCAGA TTTAACTAAC 
GTGCCTAGCC ACGTTTTCGG AGAACACAAG GACTGTGAAA GATTGGCATA TTTCTGCAAC 
CAGTACTCTG CTCCCAACGT AATAAACTAT GTTCCGCAAC TGAAGGAAGC AGGTATTTAT 
AAACGTATAG AGGAAGCCAT GCAGCCGCTT TTCAAGAATA TTCAAAGTTT GCTGTACTGT 
ATGAATAATA ATGCAGCAGA AGTCTTTAAT GCCATCATCG CGAAATACAT TGGTGGCAAA 
CGAATAAACT TTGGATTAAA AGGCTCGTAT CAGGGTAGGG TTTACGCTGC TGTCGTTCAG 
TACAATACAA AAGAGGCAGT GAGCCGAATG TGTAGAGCAA TAAATAAGGA ACCGCCAGTC 
CTTGTCAGGA AGATTGAAGA ACGACGTAAA AGCGAGAATA GAAGATATTA TATATCTTCC 
AGGCGGCGTA AAAGACAAAT GTGTGATAAG GATTACGGTC CTTGTGCGGA ACAACCTGAG 
ATAGAAGAAA TAGTTTTCAC TCCCGAGTAT GAGAAAAAAA TAGCAGCACT CCACAAATGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105663106 ( XM_012290282.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012145672.1 Megachile rotundata uncharacterized LOC105663106 (LOC105663106), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OMe108975
Clone ID Related Accession (Same CDS sequence)	XM_012290283.1 , XM_012290284.1 , XM_012290282.1 , XM_012290285.1
Accession Version	XM_012290282.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	uncharacterized protein LOC105663106 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797277.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAACG ACGATCAAAA TAATGAAGCA AATCAACAAA CTGACCAAAC TAAAGTTGGC 
GAAATAAAAG CAGATGGTGA ACACAACGAA ATAAAAACAA ATGCCGAATG CAACGAAATA 
AAAACAGATG CTGAATGCAA CGAAACAAAA GCAGATGATT GTAGAATTAT CTGCTACCAA 
AGTCTTCCAC TCAATAATAA AGGCTCTCGC AAGTGCAGTC TAAACTGTTT TCCTCTATGT 
AAAATATATG AAGACTATAA AGGAAAGCTC GAGGAGCTTA AAATTATTAA AGGAAGTATG 
GAACTAATCG ACTTCAATGA AGAGGAACAA CGTGAAATGA GCAGCATCAA CGGAACTATG 
CAAGTATACG CTATAATACC GAAAGAGAAT GAAAAAGATA TATTGGATGA AACGCAAGAA 
ACATCAAATC TAAATAATGA TTACGAGGCT TTAAATCAAG AAATTATGTC TTTAAGCCAA 
GAGACTATAC CTTCGAGTCA GGAAACTATT TCGGATGATA CTGACCTCGA ATGCAATTGG 
ATAGTAGAAA AAGCAAAACC GCAAACGCAG GAACAACCAA TTTTGGAAGG AATAGATTTT 
GTCGATTTTA AATATGTTTT TAATCAATTA TTAACACTCG ACGATCATTC CAACATCGGA 
TGCAGCATTC GACATTTTAA AATAATTGGA CGTTACCGGA ATGGACTGAA AATAACATAT 
TCTGTAAAAT GTGAAATGTG CAATCAGATA ATACATATAC CATGCATTCA ACAGAATAAT 
GACGTTATAG ATATCAATGA GAGCGCTGTT TCCGCGACAA TGGTATTAGG CGGCGGATAT 
GTTCAACTGA TGGAGATGTT GGCTTGTCTG AATGTGTCGT GTATGTCCAA ACAATCATAC 
GACAAACACA GAACAAAAAT AATAGAGGCC TTCGAACTGG CTGCACAGTG GGAAATGGAA 
GCTGCTGGTA AAAAAGAGAG AGAACTAGCT ATAGCAAGAG GTGATGTATG TCCAGACTCT 
GGCATACCAT GGATTCCAGT TGTCGCTGAT GGTAGCTGGA TGAAAAGATC ATACAAAGGT 
GGTTATTATA ATTCTCCTTC CGGTGTAGGA GTTATTGTTG GATATTATAC AAAGAAAGTG 
TTATATGTTG GCGTAAAGAA TAAAGTCTGC ATAACTTGCC GGGTAGCGGC TAAGAAAAAA 
GAAAAACCTA GCGAGCATCG TTGCTTCAAA AATTGGGGTA CAACTCAGAG TTCAACTGCA 
ATGGAAAGCG TTGCTATTTT GGAGGGATTT GAATGCAGCA TAAAAATGCA CGGCCTGATA 
TATTCAATAT TAATTGCCGA TGGTGATAGC AGTGTCTACA AAAAGATATT GGACCGTGAT 
CCGTATAAAC AGTACTCGGT GCGTGTACAA AAAATTGAAT GTATCAATCA CATGCTACGC 
AATTTCGGTA AGAAAATTGC TAATATCGGC ACTACAGGTG GGTGCCAAAA ATTAAGAGCT 
GTCGTAAAAA AAAACGCTCT CCGTCTGCGC GCTTGTATAA GGAAAGCTGC ACAATACCGC 
TATAACGAAA ACCTGCCACT GGTTGAAAAA CTTGAAAGAC TAAAAGCAGA TTTAACTAAC 
GTGCCTAGCC ACGTTTTCGG AGAACACAAG GACTGTGAAA GATTGGCATA TTTCTGCAAC 
CAGTACTCTG CTCCCAACGT AATAAACTAT GTTCCGCAAC TGAAGGAAGC AGGTATTTAT 
AAACGTATAG AGGAAGCCAT GCAGCCGCTT TTCAAGAATA TTCAAAGTTT GCTGTACTGT 
ATGAATAATA ATGCAGCAGA AGTCTTTAAT GCCATCATCG CGAAATACAT TGGTGGCAAA 
CGAATAAACT TTGGATTAAA AGGCTCGTAT CAGGGTAGGG TTTACGCTGC TGTCGTTCAG 
TACAATACAA AAGAGGCAGT GAGCCGAATG TGTAGAGCAA TAAATAAGGA ACCGCCAGTC 
CTTGTCAGGA AGATTGAAGA ACGACGTAAA AGCGAGAATA GAAGATATTA TATATCTTCC 
AGGCGGCGTA AAAGACAAAT GTGTGATAAG GATTACGGTC CTTGTGCGGA ACAACCTGAG 
ATAGAAGAAA TAGTTTTCAC TCCCGAGTAT GAGAAAAAAA TAGCAGCACT CCACAAATGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012145672.1
CDS	1618..3780
Translation	MSNDDQNNEANQQTDQTKVGEIKADGEHNEIKTNAECNEIKTDAECNETKADDCRIICYQSLPLNNKGSRKCSLNCFPLCKIYEDYKGKLEELKIIKGSMELIDFNEEEQREMSSINGTMQVYAIIPKENEKDILDETQETSNLNNDYEALNQEIMSLSQETIPSSQETISDDTDLECNWIVEKAKPQTQEQPILEGIDFVDFKYVFNQLLTLDDHSNIGCSIRHFKIIGRYRNGLKITYSVKCEMCNQIIHIPCIQQNNDVIDINESAVSATMVLGGGYVQLMEMLACLNVSCMSKQSYDKHRTKIIEAFELAAQWEMEAAGKKERELAIARGDVCPDSGIPWIPVVADGSWMKRSYKGGYYNSPSGVGVIVGYYTKKVLYVGVKNKVCITCRVAAKKKEKPSEHRCFKNWGTTQSSTAMESVAILEGFECSIKMHGLIYSILIADGDSSVYKKILDRDPYKQYSVRVQKIECINHMLRNFGKKIANIGTTGGCQKLRAVVKKNALRLRACIRKAAQYRYNENLPLVEKLERLKADLTNVPSHVFGEHKDCERLAYFCNQYSAPNVINYVPQLKEAGIYKRIEEAMQPLFKNIQSLLYCMNNNAAEVFNAIIAKYIGGKRINFGLKGSYQGRVYAAVVQYNTKEAVSRMCRAINKEPPVLVRKIEERRKSENRRYYISSRRRKRQMCDKDYGPCAEQPEIEEIVFTPEYEKKIAALHKC

Target ORF information:

RefSeq Version	XM_012290282.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata uncharacterized LOC105663106 (LOC105663106), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012290282.1

ORF Insert Sequence:

ATGTCAAACG ACGATCAAAA TAATGAAGCA AATCAACAAA CTGACCAAAC TAAAGTTGGC 
GAAATAAAAG CAGATGGTGA ACACAACGAA ATAAAAACAA ATGCCGAATG CAACGAAATA 
AAAACAGATG CTGAATGCAA CGAAACAAAA GCAGATGATT GTAGAATTAT CTGCTACCAA 
AGTCTTCCAC TCAATAATAA AGGCTCTCGC AAGTGCAGTC TAAACTGTTT TCCTCTATGT 
AAAATATATG AAGACTATAA AGGAAAGCTC GAGGAGCTTA AAATTATTAA AGGAAGTATG 
GAACTAATCG ACTTCAATGA AGAGGAACAA CGTGAAATGA GCAGCATCAA CGGAACTATG 
CAAGTATACG CTATAATACC GAAAGAGAAT GAAAAAGATA TATTGGATGA AACGCAAGAA 
ACATCAAATC TAAATAATGA TTACGAGGCT TTAAATCAAG AAATTATGTC TTTAAGCCAA 
GAGACTATAC CTTCGAGTCA GGAAACTATT TCGGATGATA CTGACCTCGA ATGCAATTGG 
ATAGTAGAAA AAGCAAAACC GCAAACGCAG GAACAACCAA TTTTGGAAGG AATAGATTTT 
GTCGATTTTA AATATGTTTT TAATCAATTA TTAACACTCG ACGATCATTC CAACATCGGA 
TGCAGCATTC GACATTTTAA AATAATTGGA CGTTACCGGA ATGGACTGAA AATAACATAT 
TCTGTAAAAT GTGAAATGTG CAATCAGATA ATACATATAC CATGCATTCA ACAGAATAAT 
GACGTTATAG ATATCAATGA GAGCGCTGTT TCCGCGACAA TGGTATTAGG CGGCGGATAT 
GTTCAACTGA TGGAGATGTT GGCTTGTCTG AATGTGTCGT GTATGTCCAA ACAATCATAC 
GACAAACACA GAACAAAAAT AATAGAGGCC TTCGAACTGG CTGCACAGTG GGAAATGGAA 
GCTGCTGGTA AAAAAGAGAG AGAACTAGCT ATAGCAAGAG GTGATGTATG TCCAGACTCT 
GGCATACCAT GGATTCCAGT TGTCGCTGAT GGTAGCTGGA TGAAAAGATC ATACAAAGGT 
GGTTATTATA ATTCTCCTTC CGGTGTAGGA GTTATTGTTG GATATTATAC AAAGAAAGTG 
TTATATGTTG GCGTAAAGAA TAAAGTCTGC ATAACTTGCC GGGTAGCGGC TAAGAAAAAA 
GAAAAACCTA GCGAGCATCG TTGCTTCAAA AATTGGGGTA CAACTCAGAG TTCAACTGCA 
ATGGAAAGCG TTGCTATTTT GGAGGGATTT GAATGCAGCA TAAAAATGCA CGGCCTGATA 
TATTCAATAT TAATTGCCGA TGGTGATAGC AGTGTCTACA AAAAGATATT GGACCGTGAT 
CCGTATAAAC AGTACTCGGT GCGTGTACAA AAAATTGAAT GTATCAATCA CATGCTACGC 
AATTTCGGTA AGAAAATTGC TAATATCGGC ACTACAGGTG GGTGCCAAAA ATTAAGAGCT 
GTCGTAAAAA AAAACGCTCT CCGTCTGCGC GCTTGTATAA GGAAAGCTGC ACAATACCGC 
TATAACGAAA ACCTGCCACT GGTTGAAAAA CTTGAAAGAC TAAAAGCAGA TTTAACTAAC 
GTGCCTAGCC ACGTTTTCGG AGAACACAAG GACTGTGAAA GATTGGCATA TTTCTGCAAC 
CAGTACTCTG CTCCCAACGT AATAAACTAT GTTCCGCAAC TGAAGGAAGC AGGTATTTAT 
AAACGTATAG AGGAAGCCAT GCAGCCGCTT TTCAAGAATA TTCAAAGTTT GCTGTACTGT 
ATGAATAATA ATGCAGCAGA AGTCTTTAAT GCCATCATCG CGAAATACAT TGGTGGCAAA 
CGAATAAACT TTGGATTAAA AGGCTCGTAT CAGGGTAGGG TTTACGCTGC TGTCGTTCAG 
TACAATACAA AAGAGGCAGT GAGCCGAATG TGTAGAGCAA TAAATAAGGA ACCGCCAGTC 
CTTGTCAGGA AGATTGAAGA ACGACGTAAA AGCGAGAATA GAAGATATTA TATATCTTCC 
AGGCGGCGTA AAAGACAAAT GTGTGATAAG GATTACGGTC CTTGTGCGGA ACAACCTGAG 
ATAGAAGAAA TAGTTTTCAC TCCCGAGTAT GAGAAAAAAA TAGCAGCACT CCACAAATGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105663106 ( XM_012290285.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012145675.1 Megachile rotundata uncharacterized LOC105663106 (LOC105663106), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OMe108975
Clone ID Related Accession (Same CDS sequence)	XM_012290283.1 , XM_012290284.1 , XM_012290282.1 , XM_012290285.1
Accession Version	XM_012290285.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	uncharacterized protein LOC105663106 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797277.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAACG ACGATCAAAA TAATGAAGCA AATCAACAAA CTGACCAAAC TAAAGTTGGC 
GAAATAAAAG CAGATGGTGA ACACAACGAA ATAAAAACAA ATGCCGAATG CAACGAAATA 
AAAACAGATG CTGAATGCAA CGAAACAAAA GCAGATGATT GTAGAATTAT CTGCTACCAA 
AGTCTTCCAC TCAATAATAA AGGCTCTCGC AAGTGCAGTC TAAACTGTTT TCCTCTATGT 
AAAATATATG AAGACTATAA AGGAAAGCTC GAGGAGCTTA AAATTATTAA AGGAAGTATG 
GAACTAATCG ACTTCAATGA AGAGGAACAA CGTGAAATGA GCAGCATCAA CGGAACTATG 
CAAGTATACG CTATAATACC GAAAGAGAAT GAAAAAGATA TATTGGATGA AACGCAAGAA 
ACATCAAATC TAAATAATGA TTACGAGGCT TTAAATCAAG AAATTATGTC TTTAAGCCAA 
GAGACTATAC CTTCGAGTCA GGAAACTATT TCGGATGATA CTGACCTCGA ATGCAATTGG 
ATAGTAGAAA AAGCAAAACC GCAAACGCAG GAACAACCAA TTTTGGAAGG AATAGATTTT 
GTCGATTTTA AATATGTTTT TAATCAATTA TTAACACTCG ACGATCATTC CAACATCGGA 
TGCAGCATTC GACATTTTAA AATAATTGGA CGTTACCGGA ATGGACTGAA AATAACATAT 
TCTGTAAAAT GTGAAATGTG CAATCAGATA ATACATATAC CATGCATTCA ACAGAATAAT 
GACGTTATAG ATATCAATGA GAGCGCTGTT TCCGCGACAA TGGTATTAGG CGGCGGATAT 
GTTCAACTGA TGGAGATGTT GGCTTGTCTG AATGTGTCGT GTATGTCCAA ACAATCATAC 
GACAAACACA GAACAAAAAT AATAGAGGCC TTCGAACTGG CTGCACAGTG GGAAATGGAA 
GCTGCTGGTA AAAAAGAGAG AGAACTAGCT ATAGCAAGAG GTGATGTATG TCCAGACTCT 
GGCATACCAT GGATTCCAGT TGTCGCTGAT GGTAGCTGGA TGAAAAGATC ATACAAAGGT 
GGTTATTATA ATTCTCCTTC CGGTGTAGGA GTTATTGTTG GATATTATAC AAAGAAAGTG 
TTATATGTTG GCGTAAAGAA TAAAGTCTGC ATAACTTGCC GGGTAGCGGC TAAGAAAAAA 
GAAAAACCTA GCGAGCATCG TTGCTTCAAA AATTGGGGTA CAACTCAGAG TTCAACTGCA 
ATGGAAAGCG TTGCTATTTT GGAGGGATTT GAATGCAGCA TAAAAATGCA CGGCCTGATA 
TATTCAATAT TAATTGCCGA TGGTGATAGC AGTGTCTACA AAAAGATATT GGACCGTGAT 
CCGTATAAAC AGTACTCGGT GCGTGTACAA AAAATTGAAT GTATCAATCA CATGCTACGC 
AATTTCGGTA AGAAAATTGC TAATATCGGC ACTACAGGTG GGTGCCAAAA ATTAAGAGCT 
GTCGTAAAAA AAAACGCTCT CCGTCTGCGC GCTTGTATAA GGAAAGCTGC ACAATACCGC 
TATAACGAAA ACCTGCCACT GGTTGAAAAA CTTGAAAGAC TAAAAGCAGA TTTAACTAAC 
GTGCCTAGCC ACGTTTTCGG AGAACACAAG GACTGTGAAA GATTGGCATA TTTCTGCAAC 
CAGTACTCTG CTCCCAACGT AATAAACTAT GTTCCGCAAC TGAAGGAAGC AGGTATTTAT 
AAACGTATAG AGGAAGCCAT GCAGCCGCTT TTCAAGAATA TTCAAAGTTT GCTGTACTGT 
ATGAATAATA ATGCAGCAGA AGTCTTTAAT GCCATCATCG CGAAATACAT TGGTGGCAAA 
CGAATAAACT TTGGATTAAA AGGCTCGTAT CAGGGTAGGG TTTACGCTGC TGTCGTTCAG 
TACAATACAA AAGAGGCAGT GAGCCGAATG TGTAGAGCAA TAAATAAGGA ACCGCCAGTC 
CTTGTCAGGA AGATTGAAGA ACGACGTAAA AGCGAGAATA GAAGATATTA TATATCTTCC 
AGGCGGCGTA AAAGACAAAT GTGTGATAAG GATTACGGTC CTTGTGCGGA ACAACCTGAG 
ATAGAAGAAA TAGTTTTCAC TCCCGAGTAT GAGAAAAAAA TAGCAGCACT CCACAAATGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012145675.1
CDS	1507..3669
Translation	MSNDDQNNEANQQTDQTKVGEIKADGEHNEIKTNAECNEIKTDAECNETKADDCRIICYQSLPLNNKGSRKCSLNCFPLCKIYEDYKGKLEELKIIKGSMELIDFNEEEQREMSSINGTMQVYAIIPKENEKDILDETQETSNLNNDYEALNQEIMSLSQETIPSSQETISDDTDLECNWIVEKAKPQTQEQPILEGIDFVDFKYVFNQLLTLDDHSNIGCSIRHFKIIGRYRNGLKITYSVKCEMCNQIIHIPCIQQNNDVIDINESAVSATMVLGGGYVQLMEMLACLNVSCMSKQSYDKHRTKIIEAFELAAQWEMEAAGKKERELAIARGDVCPDSGIPWIPVVADGSWMKRSYKGGYYNSPSGVGVIVGYYTKKVLYVGVKNKVCITCRVAAKKKEKPSEHRCFKNWGTTQSSTAMESVAILEGFECSIKMHGLIYSILIADGDSSVYKKILDRDPYKQYSVRVQKIECINHMLRNFGKKIANIGTTGGCQKLRAVVKKNALRLRACIRKAAQYRYNENLPLVEKLERLKADLTNVPSHVFGEHKDCERLAYFCNQYSAPNVINYVPQLKEAGIYKRIEEAMQPLFKNIQSLLYCMNNNAAEVFNAIIAKYIGGKRINFGLKGSYQGRVYAAVVQYNTKEAVSRMCRAINKEPPVLVRKIEERRKSENRRYYISSRRRKRQMCDKDYGPCAEQPEIEEIVFTPEYEKKIAALHKC

Target ORF information:

RefSeq Version	XM_012290285.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata uncharacterized LOC105663106 (LOC105663106), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012290285.1

ORF Insert Sequence:

ATGTCAAACG ACGATCAAAA TAATGAAGCA AATCAACAAA CTGACCAAAC TAAAGTTGGC 
GAAATAAAAG CAGATGGTGA ACACAACGAA ATAAAAACAA ATGCCGAATG CAACGAAATA 
AAAACAGATG CTGAATGCAA CGAAACAAAA GCAGATGATT GTAGAATTAT CTGCTACCAA 
AGTCTTCCAC TCAATAATAA AGGCTCTCGC AAGTGCAGTC TAAACTGTTT TCCTCTATGT 
AAAATATATG AAGACTATAA AGGAAAGCTC GAGGAGCTTA AAATTATTAA AGGAAGTATG 
GAACTAATCG ACTTCAATGA AGAGGAACAA CGTGAAATGA GCAGCATCAA CGGAACTATG 
CAAGTATACG CTATAATACC GAAAGAGAAT GAAAAAGATA TATTGGATGA AACGCAAGAA 
ACATCAAATC TAAATAATGA TTACGAGGCT TTAAATCAAG AAATTATGTC TTTAAGCCAA 
GAGACTATAC CTTCGAGTCA GGAAACTATT TCGGATGATA CTGACCTCGA ATGCAATTGG 
ATAGTAGAAA AAGCAAAACC GCAAACGCAG GAACAACCAA TTTTGGAAGG AATAGATTTT 
GTCGATTTTA AATATGTTTT TAATCAATTA TTAACACTCG ACGATCATTC CAACATCGGA 
TGCAGCATTC GACATTTTAA AATAATTGGA CGTTACCGGA ATGGACTGAA AATAACATAT 
TCTGTAAAAT GTGAAATGTG CAATCAGATA ATACATATAC CATGCATTCA ACAGAATAAT 
GACGTTATAG ATATCAATGA GAGCGCTGTT TCCGCGACAA TGGTATTAGG CGGCGGATAT 
GTTCAACTGA TGGAGATGTT GGCTTGTCTG AATGTGTCGT GTATGTCCAA ACAATCATAC 
GACAAACACA GAACAAAAAT AATAGAGGCC TTCGAACTGG CTGCACAGTG GGAAATGGAA 
GCTGCTGGTA AAAAAGAGAG AGAACTAGCT ATAGCAAGAG GTGATGTATG TCCAGACTCT 
GGCATACCAT GGATTCCAGT TGTCGCTGAT GGTAGCTGGA TGAAAAGATC ATACAAAGGT 
GGTTATTATA ATTCTCCTTC CGGTGTAGGA GTTATTGTTG GATATTATAC AAAGAAAGTG 
TTATATGTTG GCGTAAAGAA TAAAGTCTGC ATAACTTGCC GGGTAGCGGC TAAGAAAAAA 
GAAAAACCTA GCGAGCATCG TTGCTTCAAA AATTGGGGTA CAACTCAGAG TTCAACTGCA 
ATGGAAAGCG TTGCTATTTT GGAGGGATTT GAATGCAGCA TAAAAATGCA CGGCCTGATA 
TATTCAATAT TAATTGCCGA TGGTGATAGC AGTGTCTACA AAAAGATATT GGACCGTGAT 
CCGTATAAAC AGTACTCGGT GCGTGTACAA AAAATTGAAT GTATCAATCA CATGCTACGC 
AATTTCGGTA AGAAAATTGC TAATATCGGC ACTACAGGTG GGTGCCAAAA ATTAAGAGCT 
GTCGTAAAAA AAAACGCTCT CCGTCTGCGC GCTTGTATAA GGAAAGCTGC ACAATACCGC 
TATAACGAAA ACCTGCCACT GGTTGAAAAA CTTGAAAGAC TAAAAGCAGA TTTAACTAAC 
GTGCCTAGCC ACGTTTTCGG AGAACACAAG GACTGTGAAA GATTGGCATA TTTCTGCAAC 
CAGTACTCTG CTCCCAACGT AATAAACTAT GTTCCGCAAC TGAAGGAAGC AGGTATTTAT 
AAACGTATAG AGGAAGCCAT GCAGCCGCTT TTCAAGAATA TTCAAAGTTT GCTGTACTGT 
ATGAATAATA ATGCAGCAGA AGTCTTTAAT GCCATCATCG CGAAATACAT TGGTGGCAAA 
CGAATAAACT TTGGATTAAA AGGCTCGTAT CAGGGTAGGG TTTACGCTGC TGTCGTTCAG 
TACAATACAA AAGAGGCAGT GAGCCGAATG TGTAGAGCAA TAAATAAGGA ACCGCCAGTC 
CTTGTCAGGA AGATTGAAGA ACGACGTAAA AGCGAGAATA GAAGATATTA TATATCTTCC 
AGGCGGCGTA AAAGACAAAT GTGTGATAAG GATTACGGTC CTTGTGCGGA ACAACCTGAG 
ATAGAAGAAA TAGTTTTCAC TCCCGAGTAT GAGAAAAAAA TAGCAGCACT CCACAAATGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

LOC105663106 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene

Clones

gRNAs

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