LOC100883875 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100883875
Entrez Gene ID	100883875
Full Name	WD repeat-containing protein 6
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012298802.1	XP_012154192.1	WD repeat-containing protein 6 isoform X2
XM_012298806.1	XP_012154196.1	WD repeat-containing protein 6 isoform X5
XM_012298805.1	XP_012154195.1	WD repeat-containing protein 6 isoform X4
XM_012298803.1	XP_012154193.1	WD repeat-containing protein 6 isoform X3
XM_012298801.1	XP_012154191.1	WD repeat-containing protein 6 isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC100883875 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100883875 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMe98865	XM_012298802.1 Latest version!	Megachile rotundata WD repeat-containing protein 6 (LOC100883875), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OMe98866	XM_012298803.1 Latest version!	Megachile rotundata WD repeat-containing protein 6 (LOC100883875), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OMe98867	XM_012298805.1 Latest version!	Megachile rotundata WD repeat-containing protein 6 (LOC100883875), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OMe98868	XM_012298806.1 Latest version!	Megachile rotundata WD repeat-containing protein 6 (LOC100883875), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OMe98864	XM_012298801.1 Latest version!	Megachile rotundata WD repeat-containing protein 6 (LOC100883875), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100883875 ( XM_012298802.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012154192.1 Megachile rotundata WD repeat-containing protein 6 (LOC100883875), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMe98865
Clone ID Related Accession (Same CDS sequence)	XM_012298802.1
Accession Version	XM_012298802.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2799bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	WD repeat-containing protein 6 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797096.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTCAA AATTGTTATG TACTGATGTG CTTGCAATTC GCTGTGTAAA TAATTTAGTA 
TTTGTAGGTG TGGGTTGTAC ATTACATATT TTTGATATTG ACACTTACAA ATTAGAAAAG 
AAAATCGATT GCCTTTATCC ATACAATATA CATGGAATTA TTGAGGGTCC TAACAATAAG 
ATATCTATAT TTGGGGCTAA CTCTTTATGT GGATATAATA TTCAGAAAAA ACAAGAAACA 
TTAATAGTTA AAGAAGAATT TAGTAAAAAT TGTCTTAATG ATTGGATAAT AACAGTGAAA 
TGGTTATCAT TTGATGGATA TGAGTATTTA GCAGTTTTAT TAGCACATAA TAACGTATGC 
ATTTATGACT GCATCAAGAA AAACTATCAA AATGTATGGT GCAAGGAAAA ATGCATTCTG 
TATGGTGGAT CAATGTTAGT CCAAAATCAA AAAGACTTGA TCATTTTTAG CGGAACTGTA 
TATCAAGAAA TATTAATATG GGAAATAAAT TATAGTTCTT CATGTACTGA AATTTCACCT 
ATTTTACATC GTTTGCATGG ACATAATGGA GTAATCTTTT CGGTCATATA TGATTCAGTT 
ACTCAACTTA TCTGTTCTAC ATCTGATGAT AGAACAGTCA GATTATGGGA AGTGAATAGC 
AATGAAACCA ATAAAAATCA TATAATTTGG AAAGATGTCA AAATTAAATT AAAAAAAACA 
ATGTTTGGTC ATACAGCAAG AGTATGGAAA TCTATAATAA GAAATGAGAC TTTAATTACC 
ATAGGAGAAG ATTCTCTCAT GTGTACTTGG TCATTAGATG GTAAATTGAT TAATAAAATT 
TGTGCACATC ATGGAGCTGC GATATGGAGC ATTGACATAT CAAGTGATAA CAAAAGCATC 
TTTACTGGTG GAGCTGATGG CGCAGTTCAT GCATGGCCTT TCCCCAGTGA TTATATCCAA 
CCAATTACAC TAGTACCTAG ACAGCATATA TCTATTTTGC CAAAATATAA TTGTTACTTA 
AAAAGTGGTA ATTTCTTAAT TTTTAATGAA AACGGGATTT TGTACATCTA TGATAGGTGG 
CAAAGTAATC CAAAAGAGTT TTTGTATCTT GAAAGGTTTA ATACATATTG CATTATGCAA 
GTTTCACTAT GCCATTCTTA TGTTTGTTTT GCATCAAAAG ATGGATATAT AGCAATATAT 
AAAGAAGCTG ATATTACAAC TAACAAAATA CTACAATTAA TTGTGGAAGA AAAAATAATG 
GAATCACAAA TACTCTCTAT ACAATGGTTA GGAAACAATA ACCTGGTAGC TTGTGGCTTA 
AATGGTGTTC TGAAGATATT CAGTTTTACT ATGGAGGGAT CAGTAACTAT TCAATCAATG 
GGCTTGTTAC CACAATGTCG AGAGCGTTGG TTGACAGCAG CAATATTATA TGAACATGTG 
TTGATATGTG GAGATAGAGC TGGAAATATG CATGTTTTTG AGGTTCAAAA ATATAATTCG 
AATGATGAGA CAAATATACT TAAAATAAAT AACAAACCTA TTCAAACTTT TCCTAAAGTT 
CATGGAAAGA TAGGTATTCA AAATTTTATA GTTTTACATT CAAAATTAAT ATCAGGAGGT 
CGTGATGGAA CGTTACGATT TTATGAAATA ACTGAACATC AAAACACAAA ATCTTTATGC 
ACTTTACATT CGGAAAAAAT GCCAATGGAT TGGATTAGTG GCAGTTTAAA AGTATCTGAC 
GACATTTTCA TATTGGGATT CAAAGAAACG GAATTCTTAG TATATAGCAT GTTTTATCGT 
AGAATCGTAA CAAGAGTTCC TTGTGGTGGT GGTCATAGAT CATGGGATTG TATTGTACTA 
AACGAACTTG TTTCATTTCT TTATATTCGT AATAAACAAG TATATATATT TGATTTTCCG 
TTAGCTGCTT TAAAAACCCC GGTTTTATTG AATGGTTTTC ATACAAAAGA AGTAAATTTC 
ATTAACCCTG TATTAAACAC AAACATGCAA AACGTGTTTA TATCTGGAGG AGAAGATGAA 
CCTGAAACCC GTTACATGGA TGCTGAAATT TATCATAGTA CTACAAGCTT ACATTGCGTA 
TTGCTTTTCG TTGCATGCGC AGATGGCTTT GTAAGAATAT TTTTGTACAA TACTAAAACA 
AAACGTATTT CGTTAAAAAT ACATGCAAAA TGCGCAATTC GTTGCATAGT AAAAACAACT 
GTCTTAACAT ATGAAGAGAA GGTAATTGCA TTAACTATGT CAACTGATGG AATCATTCGT 
TTTATTGATT TCACTGATAT CGTTTTAAAA ATTATACAAG AGCCGCATTG TGAGAAGCAA 
GAATTTCAGA ACTATATAGA TGTATCTGTT GCGAAATTTA ATTTACATCA ATCTGGAATT 
AATTCATATG ACTTAAAAGC AATTGGAAAA GACGAATATC TTTTGGCAAC TGGTGGTGAC 
GACAATTTAT TTAACATTAT TCGTTTTAAA ATTGAAACAT CAGGCAAAGA TCAACAGTTA 
TACGTTTCAT TGTTATCAAA ATGGTGTACG TCCACTACAC ATTTTGCACA AATTACAGGA 
GTAAAATTTT ATGAAGATAA AATTTTTAGT GTTGGAATGG ATCAACAAGT CATTATGTAT 
AATTATAGCT TTAATAATGA TTGTTTATGT GTAACAGTTT TAAAGAAAAT CTTCACATTT 
GTAACAGATG TAAAAGGTCT AACTTTATGT TATAATTCAA AAGATAAGCC ATTTTTGTGT 
GTTTATGGAA GAGGATTTGA AATATTAACG TATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012154192.1
CDS	596..3394
Translation	MISKLLCTDVLAIRCVNNLVFVGVGCTLHIFDIDTYKLEKKIDCLYPYNIHGIIEGPNNKISIFGANSLCGYNIQKKQETLIVKEEFSKNCLNDWIITVKWLSFDGYEYLAVLLAHNNVCIYDCIKKNYQNVWCKEKCILYGGSMLVQNQKDLIIFSGTVYQEILIWEINYSSSCTEISPILHRLHGHNGVIFSVIYDSVTQLICSTSDDRTVRLWEVNSNETNKNHIIWKDVKIKLKKTMFGHTARVWKSIIRNETLITIGEDSLMCTWSLDGKLINKICAHHGAAIWSIDISSDNKSIFTGGADGAVHAWPFPSDYIQPITLVPRQHISILPKYNCYLKSGNFLIFNENGILYIYDRWQSNPKEFLYLERFNTYCIMQVSLCHSYVCFASKDGYIAIYKEADITTNKILQLIVEEKIMESQILSIQWLGNNNLVACGLNGVLKIFSFTMEGSVTIQSMGLLPQCRERWLTAAILYEHVLICGDRAGNMHVFEVQKYNSNDETNILKINNKPIQTFPKVHGKIGIQNFIVLHSKLISGGRDGTLRFYEITEHQNTKSLCTLHSEKMPMDWISGSLKVSDDIFILGFKETEFLVYSMFYRRIVTRVPCGGGHRSWDCIVLNELVSFLYIRNKQVYIFDFPLAALKTPVLLNGFHTKEVNFINPVLNTNMQNVFISGGEDEPETRYMDAEIYHSTTSLHCVLLFVACADGFVRIFLYNTKTKRISLKIHAKCAIRCIVKTTVLTYEEKVIALTMSTDGIIRFIDFTDIVLKIIQEPHCEKQEFQNYIDVSVAKFNLHQSGINSYDLKAIGKDEYLLATGGDDNLFNIIRFKIETSGKDQQLYVSLLSKWCTSTTHFAQITGVKFYEDKIFSVGMDQQVIMYNYSFNNDCLCVTVLKKIFTFVTDVKGLTLCYNSKDKPFLCVYGRGFEILTYE

Target ORF information:

RefSeq Version	XM_012298802.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata WD repeat-containing protein 6 (LOC100883875), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012298802.1

ORF Insert Sequence:

ATGATTTCAA AATTGTTATG TACTGATGTG CTTGCAATTC GCTGTGTAAA TAATTTAGTA 
TTTGTAGGTG TGGGTTGTAC ATTACATATT TTTGATATTG ACACTTACAA ATTAGAAAAG 
AAAATCGATT GCCTTTATCC ATACAATATA CATGGAATTA TTGAGGGTCC TAACAATAAG 
ATATCTATAT TTGGGGCTAA CTCTTTATGT GGATATAATA TTCAGAAAAA ACAAGAAACA 
TTAATAGTTA AAGAAGAATT TAGTAAAAAT TGTCTTAATG ATTGGATAAT AACAGTGAAA 
TGGTTATCAT TTGATGGATA TGAGTATTTA GCAGTTTTAT TAGCACATAA TAACGTATGC 
ATTTATGACT GCATCAAGAA AAACTATCAA AATGTATGGT GCAAGGAAAA ATGCATTCTG 
TATGGTGGAT CAATGTTAGT CCAAAATCAA AAAGACTTGA TCATTTTTAG CGGAACTGTA 
TATCAAGAAA TATTAATATG GGAAATAAAT TATAGTTCTT CATGTACTGA AATTTCACCT 
ATTTTACATC GTTTGCATGG ACATAATGGA GTAATCTTTT CGGTCATATA TGATTCAGTT 
ACTCAACTTA TCTGTTCTAC ATCTGATGAT AGAACAGTCA GATTATGGGA AGTGAATAGC 
AATGAAACCA ATAAAAATCA TATAATTTGG AAAGATGTCA AAATTAAATT AAAAAAAACA 
ATGTTTGGTC ATACAGCAAG AGTATGGAAA TCTATAATAA GAAATGAGAC TTTAATTACC 
ATAGGAGAAG ATTCTCTCAT GTGTACTTGG TCATTAGATG GTAAATTGAT TAATAAAATT 
TGTGCACATC ATGGAGCTGC GATATGGAGC ATTGACATAT CAAGTGATAA CAAAAGCATC 
TTTACTGGTG GAGCTGATGG CGCAGTTCAT GCATGGCCTT TCCCCAGTGA TTATATCCAA 
CCAATTACAC TAGTACCTAG ACAGCATATA TCTATTTTGC CAAAATATAA TTGTTACTTA 
AAAAGTGGTA ATTTCTTAAT TTTTAATGAA AACGGGATTT TGTACATCTA TGATAGGTGG 
CAAAGTAATC CAAAAGAGTT TTTGTATCTT GAAAGGTTTA ATACATATTG CATTATGCAA 
GTTTCACTAT GCCATTCTTA TGTTTGTTTT GCATCAAAAG ATGGATATAT AGCAATATAT 
AAAGAAGCTG ATATTACAAC TAACAAAATA CTACAATTAA TTGTGGAAGA AAAAATAATG 
GAATCACAAA TACTCTCTAT ACAATGGTTA GGAAACAATA ACCTGGTAGC TTGTGGCTTA 
AATGGTGTTC TGAAGATATT CAGTTTTACT ATGGAGGGAT CAGTAACTAT TCAATCAATG 
GGCTTGTTAC CACAATGTCG AGAGCGTTGG TTGACAGCAG CAATATTATA TGAACATGTG 
TTGATATGTG GAGATAGAGC TGGAAATATG CATGTTTTTG AGGTTCAAAA ATATAATTCG 
AATGATGAGA CAAATATACT TAAAATAAAT AACAAACCTA TTCAAACTTT TCCTAAAGTT 
CATGGAAAGA TAGGTATTCA AAATTTTATA GTTTTACATT CAAAATTAAT ATCAGGAGGT 
CGTGATGGAA CGTTACGATT TTATGAAATA ACTGAACATC AAAACACAAA ATCTTTATGC 
ACTTTACATT CGGAAAAAAT GCCAATGGAT TGGATTAGTG GCAGTTTAAA AGTATCTGAC 
GACATTTTCA TATTGGGATT CAAAGAAACG GAATTCTTAG TATATAGCAT GTTTTATCGT 
AGAATCGTAA CAAGAGTTCC TTGTGGTGGT GGTCATAGAT CATGGGATTG TATTGTACTA 
AACGAACTTG TTTCATTTCT TTATATTCGT AATAAACAAG TATATATATT TGATTTTCCG 
TTAGCTGCTT TAAAAACCCC GGTTTTATTG AATGGTTTTC ATACAAAAGA AGTAAATTTC 
ATTAACCCTG TATTAAACAC AAACATGCAA AACGTGTTTA TATCTGGAGG AGAAGATGAA 
CCTGAAACCC GTTACATGGA TGCTGAAATT TATCATAGTA CTACAAGCTT ACATTGCGTA 
TTGCTTTTCG TTGCATGCGC AGATGGCTTT GTAAGAATAT TTTTGTACAA TACTAAAACA 
AAACGTATTT CGTTAAAAAT ACATGCAAAA TGCGCAATTC GTTGCATAGT AAAAACAACT 
GTCTTAACAT ATGAAGAGAA GGTAATTGCA TTAACTATGT CAACTGATGG AATCATTCGT 
TTTATTGATT TCACTGATAT CGTTTTAAAA ATTATACAAG AGCCGCATTG TGAGAAGCAA 
GAATTTCAGA ACTATATAGA TGTATCTGTT GCGAAATTTA ATTTACATCA ATCTGGAATT 
AATTCATATG ACTTAAAAGC AATTGGAAAA GACGAATATC TTTTGGCAAC TGGTGGTGAC 
GACAATTTAT TTAACATTAT TCGTTTTAAA ATTGAAACAT CAGGCAAAGA TCAACAGTTA 
TACGTTTCAT TGTTATCAAA ATGGTGTACG TCCACTACAC ATTTTGCACA AATTACAGGA 
GTAAAATTTT ATGAAGATAA AATTTTTAGT GTTGGAATGG ATCAACAAGT CATTATGTAT 
AATTATAGCT TTAATAATGA TTGTTTATGT GTAACAGTTT TAAAGAAAAT CTTCACATTT 
GTAACAGATG TAAAAGGTCT AACTTTATGT TATAATTCAA AAGATAAGCC ATTTTTGTGT 
GTTTATGGAA GAGGATTTGA AATATTAACG TATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100883875 ( XM_012298803.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012154193.1 Megachile rotundata WD repeat-containing protein 6 (LOC100883875), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMe98866
Clone ID Related Accession (Same CDS sequence)	XM_012298803.1
Accession Version	XM_012298803.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	WD repeat-containing protein 6 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797096.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGCAT CAAGAAAAAC TATCAAAATG TATGGTGGAT CAATGTTAGT CCAAAATCAA 
AAAGACTTGA TCATTTTTAG CGGAACTGTA TATCAAGAAA TATTAATATG GGAAATAAAT 
TATAGTTCTT CATGTACTGA AATTTCACCT ATTTTACATC GTTTGCATGG ACATAATGGA 
GTAATCTTTT CGGTCATATA TGATTCAGTT ACTCAACTTA TCTGTTCTAC ATCTGATGAT 
AGAACAGTCA GATTATGGGA AGTGAATAGC AATGAAACCA ATAAAAATCA TATAATTTGG 
AAAGATGTCA AAATTAAATT AAAAAAAACA ATGTTTGGTC ATACAGCAAG AGTATGGAAA 
TCTATAATAA GAAATGAGAC TTTAATTACC ATAGGAGAAG ATTCTCTCAT GTGTACTTGG 
TCATTAGATG GTAAATTGAT TAATAAAATT TGTGCACATC ATGGAGCTGC GATATGGAGC 
ATTGACATAT CAAGTGATAA CAAAAGCATC TTTACTGGTG GAGCTGATGG CGCAGTTCAT 
GCATGGCCTT TCCCCAGTGA TTATATCCAA CCAATTACAC TAGTACCTAG ACAGCATATA 
TCTATTTTGC CAAAATATAA TTGTTACTTA AAAAGTGGTA ATTTCTTAAT TTTTAATGAA 
AACGGGATTT TGTACATCTA TGATAGGTGG CAAAGTAATC CAAAAGAGTT TTTGTATCTT 
GAAAGGTTTA ATACATATTG CATTATGCAA GTTTCACTAT GCCATTCTTA TGTTTGTTTT 
GCATCAAAAG ATGGATATAT AGCAATATAT AAAGAAGCTG ATATTACAAC TAACAAAATA 
CTACAATTAA TTGTGGAAGA AAAAATAATG GAATCACAAA TACTCTCTAT ACAATGGTTA 
GGAAACAATA ACCTGGTAGC TTGTGGCTTA AATGGTGTTC TGAAGATATT CAGTTTTACT 
ATGGAGGGAT CAGTAACTAT TCAATCAATG GGCTTGTTAC CACAATGTCG AGAGCGTTGG 
TTGACAGCAG CAATATTATA TGAACATGTG TTGATATGTG GAGATAGAGC TGGAAATATG 
CATGTTTTTG AGGTTCAAAA ATATAATTCG AATGATGAGA CAAATATACT TAAAATAAAT 
AACAAACCTA TTCAAACTTT TCCTAAAGTT CATGGAAAGA TAGGTATTCA AAATTTTATA 
GTTTTACATT CAAAATTAAT ATCAGGAGGT CGTGATGGAA CGTTACGATT TTATGAAATA 
ACTGAACATC AAAACACAAA ATCTTTATGC ACTTTACATT CGGAAAAAAT GCCAATGGAT 
TGGATTAGTG GCAGTTTAAA AGTATCTGAC GACATTTTCA TATTGGGATT CAAAGAAACG 
GAATTCTTAG TATATAGCAT GTTTTATCGT AGAATCGTAA CAAGAGTTCC TTGTGGTGGT 
GGTCATAGAT CATGGGATTG TATTGTACTA AACGAACTTG TTTCATTTCT TTATATTCGT 
AATAAACAAG TATATATATT TGATTTTCCG TTAGCTGCTT TAAAAACCCC GGTTTTATTG 
AATGGTTTTC ATACAAAAGA AGTAAATTTC ATTAACCCTG TATTAAACAC AAACATGCAA 
AACGTGTTTA TATCTGGAGG AGAAGATGGT ACTCTTCATA TTTCTCATGT CTTCAGTACA 
TTAGTAAAAA ATAATTTGTG CTTACAAACA TTAGGTATTT TTGATGGCCA CATTTCCACA 
GTAAAATTTA TAACTTCCTT AAGTTTGCAA TTTAATTCTT CCCGCAATAA ACATCTTGTT 
TTTTCTGGGG GTGGAAGGGC CCAAATAAAA GTGTGGGAAA TTAATATAAA CGATAGCAAA 
AAATCATTAC AAAGTATAGA TATTTCTTGT CATGACGTTA CATCTCATAT GTTGTACGGA 
TTTGATCGAT ATCGTAAAAA ACAATGTCAG GAGTCTAATC GGTCTTACAT CGTAGAACCT 
GAAACCCGTT ACATGGATGC TGAAATTTAT CATAGTACTA CAAGCTTACA TTGCGTATTG 
CTTTTCGTTG CATGCGCAGA TGGCTTTGTA AGAATATTTT TGTACAATAC TAAAACAAAA 
CGTATTTCGT TAAAAATACA TGCAAAATGC GCAATTCGTT GCATAGTAAA AACAACTGTC 
TTAACATATG AAGAGAAGGT AATTGCATTA ACTATGTCAA CTGATGGAAT CATTCGTTTT 
ATTGATTTCA CTGATATCGT TTTAAAAATT ATACAAGAGC CGCATTGTGA GAAGCAAGAA 
TTTCAGAACT ATATAGATGT ATCTGTTGCG AAATTTAATT TACATCAATC TGGAATTAAT 
TCATATGACT TAAAAGCAAT TGGAAAAGAC GAATATCTTT TGGCAACTGG TGGTGACGAC 
AATTTATTTA ACATTATTCG TTTTAAAATT GAAACATCAG GCAAAGATCA ACAGTTATAC 
GTTTCATTGT TATCAAAATG GTGTACGTCC ACTACACATT TTGCACAAAT TACAGGAGTA 
AAATTTTATG AAGATAAAAT TTTTAGTGTT GGAATGGATC AACAAGTCAT TATGTATAAT 
TATAGCTTTA ATAATGATTG TTTATGTGTA ACAGTTTTAA AGAAAATCTT CACATTTGTA 
ACAGATGTAA AAGGTCTAAC TTTATGTTAT AATTCAAAAG ATAAGCCATT TTTGTGTGTT 
TATGGAAGAG GATTTGAAAT ATTAACGTAT GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012154193.1
CDS	88..2823
Translation	MTASRKTIKMYGGSMLVQNQKDLIIFSGTVYQEILIWEINYSSSCTEISPILHRLHGHNGVIFSVIYDSVTQLICSTSDDRTVRLWEVNSNETNKNHIIWKDVKIKLKKTMFGHTARVWKSIIRNETLITIGEDSLMCTWSLDGKLINKICAHHGAAIWSIDISSDNKSIFTGGADGAVHAWPFPSDYIQPITLVPRQHISILPKYNCYLKSGNFLIFNENGILYIYDRWQSNPKEFLYLERFNTYCIMQVSLCHSYVCFASKDGYIAIYKEADITTNKILQLIVEEKIMESQILSIQWLGNNNLVACGLNGVLKIFSFTMEGSVTIQSMGLLPQCRERWLTAAILYEHVLICGDRAGNMHVFEVQKYNSNDETNILKINNKPIQTFPKVHGKIGIQNFIVLHSKLISGGRDGTLRFYEITEHQNTKSLCTLHSEKMPMDWISGSLKVSDDIFILGFKETEFLVYSMFYRRIVTRVPCGGGHRSWDCIVLNELVSFLYIRNKQVYIFDFPLAALKTPVLLNGFHTKEVNFINPVLNTNMQNVFISGGEDGTLHISHVFSTLVKNNLCLQTLGIFDGHISTVKFITSLSLQFNSSRNKHLVFSGGGRAQIKVWEININDSKKSLQSIDISCHDVTSHMLYGFDRYRKKQCQESNRSYIVEPETRYMDAEIYHSTTSLHCVLLFVACADGFVRIFLYNTKTKRISLKIHAKCAIRCIVKTTVLTYEEKVIALTMSTDGIIRFIDFTDIVLKIIQEPHCEKQEFQNYIDVSVAKFNLHQSGINSYDLKAIGKDEYLLATGGDDNLFNIIRFKIETSGKDQQLYVSLLSKWCTSTTHFAQITGVKFYEDKIFSVGMDQQVIMYNYSFNNDCLCVTVLKKIFTFVTDVKGLTLCYNSKDKPFLCVYGRGFEILTYE

Target ORF information:

RefSeq Version	XM_012298803.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata WD repeat-containing protein 6 (LOC100883875), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012298803.1

ORF Insert Sequence:

ATGACTGCAT CAAGAAAAAC TATCAAAATG TATGGTGGAT CAATGTTAGT CCAAAATCAA 
AAAGACTTGA TCATTTTTAG CGGAACTGTA TATCAAGAAA TATTAATATG GGAAATAAAT 
TATAGTTCTT CATGTACTGA AATTTCACCT ATTTTACATC GTTTGCATGG ACATAATGGA 
GTAATCTTTT CGGTCATATA TGATTCAGTT ACTCAACTTA TCTGTTCTAC ATCTGATGAT 
AGAACAGTCA GATTATGGGA AGTGAATAGC AATGAAACCA ATAAAAATCA TATAATTTGG 
AAAGATGTCA AAATTAAATT AAAAAAAACA ATGTTTGGTC ATACAGCAAG AGTATGGAAA 
TCTATAATAA GAAATGAGAC TTTAATTACC ATAGGAGAAG ATTCTCTCAT GTGTACTTGG 
TCATTAGATG GTAAATTGAT TAATAAAATT TGTGCACATC ATGGAGCTGC GATATGGAGC 
ATTGACATAT CAAGTGATAA CAAAAGCATC TTTACTGGTG GAGCTGATGG CGCAGTTCAT 
GCATGGCCTT TCCCCAGTGA TTATATCCAA CCAATTACAC TAGTACCTAG ACAGCATATA 
TCTATTTTGC CAAAATATAA TTGTTACTTA AAAAGTGGTA ATTTCTTAAT TTTTAATGAA 
AACGGGATTT TGTACATCTA TGATAGGTGG CAAAGTAATC CAAAAGAGTT TTTGTATCTT 
GAAAGGTTTA ATACATATTG CATTATGCAA GTTTCACTAT GCCATTCTTA TGTTTGTTTT 
GCATCAAAAG ATGGATATAT AGCAATATAT AAAGAAGCTG ATATTACAAC TAACAAAATA 
CTACAATTAA TTGTGGAAGA AAAAATAATG GAATCACAAA TACTCTCTAT ACAATGGTTA 
GGAAACAATA ACCTGGTAGC TTGTGGCTTA AATGGTGTTC TGAAGATATT CAGTTTTACT 
ATGGAGGGAT CAGTAACTAT TCAATCAATG GGCTTGTTAC CACAATGTCG AGAGCGTTGG 
TTGACAGCAG CAATATTATA TGAACATGTG TTGATATGTG GAGATAGAGC TGGAAATATG 
CATGTTTTTG AGGTTCAAAA ATATAATTCG AATGATGAGA CAAATATACT TAAAATAAAT 
AACAAACCTA TTCAAACTTT TCCTAAAGTT CATGGAAAGA TAGGTATTCA AAATTTTATA 
GTTTTACATT CAAAATTAAT ATCAGGAGGT CGTGATGGAA CGTTACGATT TTATGAAATA 
ACTGAACATC AAAACACAAA ATCTTTATGC ACTTTACATT CGGAAAAAAT GCCAATGGAT 
TGGATTAGTG GCAGTTTAAA AGTATCTGAC GACATTTTCA TATTGGGATT CAAAGAAACG 
GAATTCTTAG TATATAGCAT GTTTTATCGT AGAATCGTAA CAAGAGTTCC TTGTGGTGGT 
GGTCATAGAT CATGGGATTG TATTGTACTA AACGAACTTG TTTCATTTCT TTATATTCGT 
AATAAACAAG TATATATATT TGATTTTCCG TTAGCTGCTT TAAAAACCCC GGTTTTATTG 
AATGGTTTTC ATACAAAAGA AGTAAATTTC ATTAACCCTG TATTAAACAC AAACATGCAA 
AACGTGTTTA TATCTGGAGG AGAAGATGGT ACTCTTCATA TTTCTCATGT CTTCAGTACA 
TTAGTAAAAA ATAATTTGTG CTTACAAACA TTAGGTATTT TTGATGGCCA CATTTCCACA 
GTAAAATTTA TAACTTCCTT AAGTTTGCAA TTTAATTCTT CCCGCAATAA ACATCTTGTT 
TTTTCTGGGG GTGGAAGGGC CCAAATAAAA GTGTGGGAAA TTAATATAAA CGATAGCAAA 
AAATCATTAC AAAGTATAGA TATTTCTTGT CATGACGTTA CATCTCATAT GTTGTACGGA 
TTTGATCGAT ATCGTAAAAA ACAATGTCAG GAGTCTAATC GGTCTTACAT CGTAGAACCT 
GAAACCCGTT ACATGGATGC TGAAATTTAT CATAGTACTA CAAGCTTACA TTGCGTATTG 
CTTTTCGTTG CATGCGCAGA TGGCTTTGTA AGAATATTTT TGTACAATAC TAAAACAAAA 
CGTATTTCGT TAAAAATACA TGCAAAATGC GCAATTCGTT GCATAGTAAA AACAACTGTC 
TTAACATATG AAGAGAAGGT AATTGCATTA ACTATGTCAA CTGATGGAAT CATTCGTTTT 
ATTGATTTCA CTGATATCGT TTTAAAAATT ATACAAGAGC CGCATTGTGA GAAGCAAGAA 
TTTCAGAACT ATATAGATGT ATCTGTTGCG AAATTTAATT TACATCAATC TGGAATTAAT 
TCATATGACT TAAAAGCAAT TGGAAAAGAC GAATATCTTT TGGCAACTGG TGGTGACGAC 
AATTTATTTA ACATTATTCG TTTTAAAATT GAAACATCAG GCAAAGATCA ACAGTTATAC 
GTTTCATTGT TATCAAAATG GTGTACGTCC ACTACACATT TTGCACAAAT TACAGGAGTA 
AAATTTTATG AAGATAAAAT TTTTAGTGTT GGAATGGATC AACAAGTCAT TATGTATAAT 
TATAGCTTTA ATAATGATTG TTTATGTGTA ACAGTTTTAA AGAAAATCTT CACATTTGTA 
ACAGATGTAA AAGGTCTAAC TTTATGTTAT AATTCAAAAG ATAAGCCATT TTTGTGTGTT 
TATGGAAGAG GATTTGAAAT ATTAACGTAT GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100883875 ( XM_012298805.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012154195.1 Megachile rotundata WD repeat-containing protein 6 (LOC100883875), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMe98867
Clone ID Related Accession (Same CDS sequence)	XM_012298805.1
Accession Version	XM_012298805.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	WD repeat-containing protein 6 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797096.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTCAA AATTGTTATG TACTGATGTG CTTGCAATTC GCTGTGTAAA TAATTTAGTA 
TTTGTAGGTG TGGGTTGTAC ATTACATATT TTTGATATTG ACACTTACAA ATTAGAAAAG 
AAAATCGATT GCCTTTATCC ATACAATATA CATGGAATTA TTGAGGGTCC TAACAATAAG 
ATATCTATAT TTGGGGCTAA CTCTTTATGT GGATATAATA TTCAGAAAAA ACAAGAAACA 
TTAATAGTTA AAGAAGAATT TAGTAAAAAT TGTCTTAATG ATTGGATAAT AACAGTGAAA 
TGGTTATCAT TTGATGGATA TGAGTATTTA GCAGTTTTAT TAGCACATAA TAACGTATGC 
ATTTATGACT GCATCAAGAA AAACTATCAA AATGTATGGT GCAAGGAAAA ATGCATTCTG 
TATGGTGGAT CAATGTTAGT CCAAAATCAA AAAGACTTGA TCATTTTTAG CGGAACTGTA 
TATCAAGAAA TATTAATATG GGAAATAAAT TATAGTTCTT CATGTACTGA AATTTCACCT 
ATTTTACATC GTTTGCATGG ACATAATGGA GTAATCTTTT CGGTCATATA TGATTCAGTT 
ACTCAACTTA TCTGTTCTAC ATCTGATGAT AGAACAGTCA GATTATGGGA AGTGAATAGC 
AATGAAACCA ATAAAAATCA TATAATTTGG AAAGATGTCA AAATTAAATT AAAAAAAACA 
ATGTTTGGTC ATACAGCAAG AGTATGGAAA TCTATAATAA GAAATGAGAC TTTAATTACC 
ATAGGAGAAG ATTCTCTCAT GTGTACTTGG TCATTAGATG GTAAATTGAT TAATAAAATT 
TGTGCACATC ATGGAGCTGC GATATGGAGC ATTGACATAT CAAGTGATAA CAAAAGCATC 
TTTACTGGTG GAGCTGATGG CGCAGTTCAT GCATGGCCTT TCCCCAGTGA TTATATCCAA 
CCAATTACAC TAGTACCTAG ACAGCATATA TCTATTTTGC CAAAATATAA TTGTTACTTA 
AAAAGTGGTA ATTTCTTAAT TTTTAATGAA AACGGGATTT TGTACATCTA TGATAGGTGG 
CAAAGTAATC CAAAAGAGTT TTTGTATCTT GAAAGGTTTA ATACATATTG CATTATGCAA 
GTTTCACTAT GCCATTCTTA TGTTTGTTTT GCATCAAAAG ATGGATATAT AGCAATATAT 
AAAGAAGCTG ATATTACAAC TAACAAAATA CTACAATTAA TTGTGGAAGA AAAAATAATG 
GAATCACAAA TACTCTCTAT ACAATGGTTA GGAAACAATA ACCTGGTAGC TTGTGGCTTA 
AATGGTGTTC TGAAGATATT CAGTTTTACT ATGGAGGGAT CAGTAACTAT TCAATCAATG 
GGCTTGTTAC CACAATGTCG AGAGCGTTGG TTGACAGCAG CAATATTATA TGAACATGTG 
TTGATATGTG GAGATAGAGC TGGAAATATG CATGTTTTTG AGGTTCAAAA ATATAATTCG 
AATGATGAGA CAAATATACT TAAAATAAAT AACAAACCTA TTCAAACTTT TCCTAAAGTT 
CATGGAAAGA TAGGTATTCA AAATTTTATA GTTTTACATT CAAAATTAAT ATCAGGAGGT 
CGTGATGGAA CGTTACGATT TTATGAAATA ACTGAACATC AAAACACAAA ATCTTTATGC 
ACTTTACATT CGGAAAAAAT GCCAATGGAT TGGATTAGTG GCAGTTTAAA AGTATCTGAC 
GACATTTTCA TATTGGGATT CAAAGAAACG GAATTCTTAG TATATAGCAT GTTTTATCGT 
AGAATCGTAA CAAGAGTTCC TTGTGGTGGT GGTCATAGAT CATGGGATTG TATTGTACTA 
AACGAACTTG TTTCATTTCT TTATATTCGT AATAAACAAG TATATATATT TGATTTTCCG 
TTAGCTGCTT TAAAAACCCC GGTTTTATTG AATGGTTTTC ATACAAAAGA AGTAAATTTC 
ATTAACCCTG TATTAAACAC AAACATGCAA AACGTGTTTA TATCTGGAGG AGAAGATGGT 
ACTCTTCATA TTTCTCATGT CTTCAGTACA TTAGTAAAAA ATAATTTGTG CTTACAAACA 
TTAGGTATTT TTGATGGCCA CATTTCCACA GTAAAATTTA TAACTTCCTT AAGTTTGCAA 
TTTAATTCTT CCCGCAATAA ACATCTTGTT TTTTCTGGGG GTGGAAGGGC CCAAATAAAA 
GTGTGGGAAA TTAATATAAA CGATAGCAAA AAATCATTAC AAAGTATAGA TATTTCTTGT 
CATGACGTTA CATCTCATAT GTTGTACGGA TTTGATCGAT ATCGTAAAAA ACAATGTCAG 
GAGTCTAATC GGTCTTACAT CGTAGAACCT GAAACCCGTT ACATGGATGC TGAAATTTAT 
CATAGTACTA CAAGCTTACA TTGCGTATTG CTTTTCGTTG CATGCGCAGA TGGCTTTGTA 
AGAATATTTT TGTACAATAC TAAAACAAAA CGTATTTCGT TAAAAATACA TGCAAAATGC 
GCAATTCGTT GCATAGTAAA AACAACTGTC TTAACATATG AAGAGAAGAG CCGCATTGTG 
AGAAGCAAGA ATTTCAGAAC TATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012154195.1
CDS	596..3202
Translation	MISKLLCTDVLAIRCVNNLVFVGVGCTLHIFDIDTYKLEKKIDCLYPYNIHGIIEGPNNKISIFGANSLCGYNIQKKQETLIVKEEFSKNCLNDWIITVKWLSFDGYEYLAVLLAHNNVCIYDCIKKNYQNVWCKEKCILYGGSMLVQNQKDLIIFSGTVYQEILIWEINYSSSCTEISPILHRLHGHNGVIFSVIYDSVTQLICSTSDDRTVRLWEVNSNETNKNHIIWKDVKIKLKKTMFGHTARVWKSIIRNETLITIGEDSLMCTWSLDGKLINKICAHHGAAIWSIDISSDNKSIFTGGADGAVHAWPFPSDYIQPITLVPRQHISILPKYNCYLKSGNFLIFNENGILYIYDRWQSNPKEFLYLERFNTYCIMQVSLCHSYVCFASKDGYIAIYKEADITTNKILQLIVEEKIMESQILSIQWLGNNNLVACGLNGVLKIFSFTMEGSVTIQSMGLLPQCRERWLTAAILYEHVLICGDRAGNMHVFEVQKYNSNDETNILKINNKPIQTFPKVHGKIGIQNFIVLHSKLISGGRDGTLRFYEITEHQNTKSLCTLHSEKMPMDWISGSLKVSDDIFILGFKETEFLVYSMFYRRIVTRVPCGGGHRSWDCIVLNELVSFLYIRNKQVYIFDFPLAALKTPVLLNGFHTKEVNFINPVLNTNMQNVFISGGEDGTLHISHVFSTLVKNNLCLQTLGIFDGHISTVKFITSLSLQFNSSRNKHLVFSGGGRAQIKVWEININDSKKSLQSIDISCHDVTSHMLYGFDRYRKKQCQESNRSYIVEPETRYMDAEIYHSTTSLHCVLLFVACADGFVRIFLYNTKTKRISLKIHAKCAIRCIVKTTVLTYEEKSRIVRSKNFRTI

Target ORF information:

RefSeq Version	XM_012298805.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata WD repeat-containing protein 6 (LOC100883875), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012298805.1

ORF Insert Sequence:

ATGATTTCAA AATTGTTATG TACTGATGTG CTTGCAATTC GCTGTGTAAA TAATTTAGTA 
TTTGTAGGTG TGGGTTGTAC ATTACATATT TTTGATATTG ACACTTACAA ATTAGAAAAG 
AAAATCGATT GCCTTTATCC ATACAATATA CATGGAATTA TTGAGGGTCC TAACAATAAG 
ATATCTATAT TTGGGGCTAA CTCTTTATGT GGATATAATA TTCAGAAAAA ACAAGAAACA 
TTAATAGTTA AAGAAGAATT TAGTAAAAAT TGTCTTAATG ATTGGATAAT AACAGTGAAA 
TGGTTATCAT TTGATGGATA TGAGTATTTA GCAGTTTTAT TAGCACATAA TAACGTATGC 
ATTTATGACT GCATCAAGAA AAACTATCAA AATGTATGGT GCAAGGAAAA ATGCATTCTG 
TATGGTGGAT CAATGTTAGT CCAAAATCAA AAAGACTTGA TCATTTTTAG CGGAACTGTA 
TATCAAGAAA TATTAATATG GGAAATAAAT TATAGTTCTT CATGTACTGA AATTTCACCT 
ATTTTACATC GTTTGCATGG ACATAATGGA GTAATCTTTT CGGTCATATA TGATTCAGTT 
ACTCAACTTA TCTGTTCTAC ATCTGATGAT AGAACAGTCA GATTATGGGA AGTGAATAGC 
AATGAAACCA ATAAAAATCA TATAATTTGG AAAGATGTCA AAATTAAATT AAAAAAAACA 
ATGTTTGGTC ATACAGCAAG AGTATGGAAA TCTATAATAA GAAATGAGAC TTTAATTACC 
ATAGGAGAAG ATTCTCTCAT GTGTACTTGG TCATTAGATG GTAAATTGAT TAATAAAATT 
TGTGCACATC ATGGAGCTGC GATATGGAGC ATTGACATAT CAAGTGATAA CAAAAGCATC 
TTTACTGGTG GAGCTGATGG CGCAGTTCAT GCATGGCCTT TCCCCAGTGA TTATATCCAA 
CCAATTACAC TAGTACCTAG ACAGCATATA TCTATTTTGC CAAAATATAA TTGTTACTTA 
AAAAGTGGTA ATTTCTTAAT TTTTAATGAA AACGGGATTT TGTACATCTA TGATAGGTGG 
CAAAGTAATC CAAAAGAGTT TTTGTATCTT GAAAGGTTTA ATACATATTG CATTATGCAA 
GTTTCACTAT GCCATTCTTA TGTTTGTTTT GCATCAAAAG ATGGATATAT AGCAATATAT 
AAAGAAGCTG ATATTACAAC TAACAAAATA CTACAATTAA TTGTGGAAGA AAAAATAATG 
GAATCACAAA TACTCTCTAT ACAATGGTTA GGAAACAATA ACCTGGTAGC TTGTGGCTTA 
AATGGTGTTC TGAAGATATT CAGTTTTACT ATGGAGGGAT CAGTAACTAT TCAATCAATG 
GGCTTGTTAC CACAATGTCG AGAGCGTTGG TTGACAGCAG CAATATTATA TGAACATGTG 
TTGATATGTG GAGATAGAGC TGGAAATATG CATGTTTTTG AGGTTCAAAA ATATAATTCG 
AATGATGAGA CAAATATACT TAAAATAAAT AACAAACCTA TTCAAACTTT TCCTAAAGTT 
CATGGAAAGA TAGGTATTCA AAATTTTATA GTTTTACATT CAAAATTAAT ATCAGGAGGT 
CGTGATGGAA CGTTACGATT TTATGAAATA ACTGAACATC AAAACACAAA ATCTTTATGC 
ACTTTACATT CGGAAAAAAT GCCAATGGAT TGGATTAGTG GCAGTTTAAA AGTATCTGAC 
GACATTTTCA TATTGGGATT CAAAGAAACG GAATTCTTAG TATATAGCAT GTTTTATCGT 
AGAATCGTAA CAAGAGTTCC TTGTGGTGGT GGTCATAGAT CATGGGATTG TATTGTACTA 
AACGAACTTG TTTCATTTCT TTATATTCGT AATAAACAAG TATATATATT TGATTTTCCG 
TTAGCTGCTT TAAAAACCCC GGTTTTATTG AATGGTTTTC ATACAAAAGA AGTAAATTTC 
ATTAACCCTG TATTAAACAC AAACATGCAA AACGTGTTTA TATCTGGAGG AGAAGATGGT 
ACTCTTCATA TTTCTCATGT CTTCAGTACA TTAGTAAAAA ATAATTTGTG CTTACAAACA 
TTAGGTATTT TTGATGGCCA CATTTCCACA GTAAAATTTA TAACTTCCTT AAGTTTGCAA 
TTTAATTCTT CCCGCAATAA ACATCTTGTT TTTTCTGGGG GTGGAAGGGC CCAAATAAAA 
GTGTGGGAAA TTAATATAAA CGATAGCAAA AAATCATTAC AAAGTATAGA TATTTCTTGT 
CATGACGTTA CATCTCATAT GTTGTACGGA TTTGATCGAT ATCGTAAAAA ACAATGTCAG 
GAGTCTAATC GGTCTTACAT CGTAGAACCT GAAACCCGTT ACATGGATGC TGAAATTTAT 
CATAGTACTA CAAGCTTACA TTGCGTATTG CTTTTCGTTG CATGCGCAGA TGGCTTTGTA 
AGAATATTTT TGTACAATAC TAAAACAAAA CGTATTTCGT TAAAAATACA TGCAAAATGC 
GCAATTCGTT GCATAGTAAA AACAACTGTC TTAACATATG AAGAGAAGAG CCGCATTGTG 
AGAAGCAAGA ATTTCAGAAC TATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100883875 ( XM_012298806.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012154196.1 Megachile rotundata WD repeat-containing protein 6 (LOC100883875), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMe98868
Clone ID Related Accession (Same CDS sequence)	XM_012298806.1
Accession Version	XM_012298806.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	WD repeat-containing protein 6 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797096.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTCAA AATTGTTATG TACTGATGTG CTTGCAATTC GCTGTGTAAA TAATTTAGTA 
TTTGTAGGTG TGGGTTGTAC ATTACATATT TTTGATATTG ACACTTACAA ATTAGAAAAG 
AAAATCGATT GCCTTTATCC ATACAATATA CATGGAATTA TTGAGGGTCC TAACAATAAG 
ATATCTATAT TTGGGGCTAA CTCTTTATGT GGATATAATA TTCAGAAAAA ACAAGAAACA 
TTAATAGTTA AAGAAGAATT TAGTAAAAAT TGTCTTAATG ATTGGATAAT AACAGTGAAA 
TGGTTATCAT TTGATGGATA TGAGTATTTA GCAGTTTTAT TAGCACATAA TAACGTATGC 
ATTTATGACT GCATCAAGAA AAACTATCAA AATGTATGGT GCAAGGAAAA ATGCATTCTG 
TATGGTGGAT CAATGTTAGT CCAAAATCAA AAAGACTTGA TCATTTTTAG CGGAACTGTA 
TATCAAGAAA TATTAATATG GGAAATAAAT TATAGTTCTT CATGTACTGA AATTTCACCT 
ATTTTACATC GTTTGCATGG ACATAATGGA GTAATCTTTT CGGTCATATA TGATTCAGTT 
ACTCAACTTA TCTGTTCTAC ATCTGATGAT AGAACAGTCA GATTATGGGA AGTGAATAGC 
AATGAAACCA ATAAAAATCA TATAATTTGG AAAGATGTCA AAATTAAATT AAAAAAAACA 
ATGTTTGGTC ATACAGCAAG AGTATGGAAA TCTATAATAA GAAATGAGAC TTTAATTACC 
ATAGGAGAAG ATTCTCTCAT GTGTACTTGG TCATTAGATG GTAAATTGAT TAATAAAATT 
TGTGCACATC ATGGAGCTGC GATATGGAGC ATTGACATAT CAAGTGATAA CAAAAGCATC 
TTTACTGGTG GAGCTGATGG CGCAGTTCAT GCATGGCCTT TCCCCAGTGA TTATATCCAA 
CCAATTACAC TAGTACCTAG ACAGCATATA TCTATTTTGC CAAAATATAA TTGTTACTTA 
AAAAGTGGTA ATTTCTTAAT TTTTAATGAA AACGGGATTT TGTACATCTA TGATAGGTGG 
CAAAGTAATC CAAAAGAGTT TTTGTATCTT GAAAGGTTTA ATACATATTG CATTATGCAA 
GTTTCACTAT GCCATTCTTA TGTTTGTTTT GCATCAAAAG ATGGATATAT AGCAATATAT 
AAAGAAGCTG ATATTACAAC TAACAAAATA CTACAATTAA TTGTGGAAGA AAAAATAATG 
GAATCACAAA TACTCTCTAT ACAATGGTTA GGAAACAATA ACCTGGTAGC TTGTGGCTTA 
AATGGTGTTC TGAAGATATT CAGTTTTACT ATGGAGGGAT CAGTAACTAT TCAATCAATG 
GGCTTGTTAC CACAATGTCG AGAGCGTTGG TTGACAGCAG CAATATTATA TGAACATGTG 
TTGATATGTG GAGATAGAGC TGGAAATATG CATGTTTTTG AGGTTCAAAA ATATAATTCG 
AATGATGAGA CAAATATACT TAAAATAAAT AACAAACCTA TTCAAACTTT TCCTAAAGTT 
CATGGAAAGA TAGAACCTGA AACCCGTTAC ATGGATGCTG AAATTTATCA TAGTACTACA 
AGCTTACATT GCGTATTGCT TTTCGTTGCA TGCGCAGATG GCTTTGTAAG AATATTTTTG 
TACAATACTA AAACAAAACG TATTTCGTTA AAAATACATG CAAAATGCGC AATTCGTTGC 
ATAGTAAAAA CAACTGTCTT AACATATGAA GAGAAGGTAA TTGCATTAAC TATGTCAACT 
GATGGAATCA TTCGTTTTAT TGATTTCACT GATATCGTTT TAAAAATTAT ACAAGAGCCG 
CATTGTGAGA AGCAAGAATT TCAGAACTAT ATAGATGTAT CTGTTGCGAA ATTTAATTTA 
CATCAATCTG GAATTAATTC ATATGACTTA AAAGCAATTG GAAAAGACGA ATATCTTTTG 
GCAACTGGTG GTGACGACAA TTTATTTAAC ATTATTCGTT TTAAAATTGA AACATCAGGC 
AAAGATCAAC AGTTATACGT TTCATTGTTA TCAAAATGGT GTACGTCCAC TACACATTTT 
GCACAAATTA CAGGAGTAAA ATTTTATGAA GATAAAATTT TTAGTGTTGG AATGGATCAA 
CAAGTCATTA TGTATAATTA TAGCTTTAAT AATGATTGTT TATGTGTAAC AGTTTTAAAG 
AAAATCTTCA CATTTGTAAC AGATGTAAAA GGTCTAACTT TATGTTATAA TTCAAAAGAT 
AAGCCATTTT TGTGTGTTTA TGGAAGAGGA TTTGAAATAT TAACGTATGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012154196.1
CDS	596..2929
Translation	MISKLLCTDVLAIRCVNNLVFVGVGCTLHIFDIDTYKLEKKIDCLYPYNIHGIIEGPNNKISIFGANSLCGYNIQKKQETLIVKEEFSKNCLNDWIITVKWLSFDGYEYLAVLLAHNNVCIYDCIKKNYQNVWCKEKCILYGGSMLVQNQKDLIIFSGTVYQEILIWEINYSSSCTEISPILHRLHGHNGVIFSVIYDSVTQLICSTSDDRTVRLWEVNSNETNKNHIIWKDVKIKLKKTMFGHTARVWKSIIRNETLITIGEDSLMCTWSLDGKLINKICAHHGAAIWSIDISSDNKSIFTGGADGAVHAWPFPSDYIQPITLVPRQHISILPKYNCYLKSGNFLIFNENGILYIYDRWQSNPKEFLYLERFNTYCIMQVSLCHSYVCFASKDGYIAIYKEADITTNKILQLIVEEKIMESQILSIQWLGNNNLVACGLNGVLKIFSFTMEGSVTIQSMGLLPQCRERWLTAAILYEHVLICGDRAGNMHVFEVQKYNSNDETNILKINNKPIQTFPKVHGKIEPETRYMDAEIYHSTTSLHCVLLFVACADGFVRIFLYNTKTKRISLKIHAKCAIRCIVKTTVLTYEEKVIALTMSTDGIIRFIDFTDIVLKIIQEPHCEKQEFQNYIDVSVAKFNLHQSGINSYDLKAIGKDEYLLATGGDDNLFNIIRFKIETSGKDQQLYVSLLSKWCTSTTHFAQITGVKFYEDKIFSVGMDQQVIMYNYSFNNDCLCVTVLKKIFTFVTDVKGLTLCYNSKDKPFLCVYGRGFEILTYE

Target ORF information:

RefSeq Version	XM_012298806.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata WD repeat-containing protein 6 (LOC100883875), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012298806.1

ORF Insert Sequence:

ATGATTTCAA AATTGTTATG TACTGATGTG CTTGCAATTC GCTGTGTAAA TAATTTAGTA 
TTTGTAGGTG TGGGTTGTAC ATTACATATT TTTGATATTG ACACTTACAA ATTAGAAAAG 
AAAATCGATT GCCTTTATCC ATACAATATA CATGGAATTA TTGAGGGTCC TAACAATAAG 
ATATCTATAT TTGGGGCTAA CTCTTTATGT GGATATAATA TTCAGAAAAA ACAAGAAACA 
TTAATAGTTA AAGAAGAATT TAGTAAAAAT TGTCTTAATG ATTGGATAAT AACAGTGAAA 
TGGTTATCAT TTGATGGATA TGAGTATTTA GCAGTTTTAT TAGCACATAA TAACGTATGC 
ATTTATGACT GCATCAAGAA AAACTATCAA AATGTATGGT GCAAGGAAAA ATGCATTCTG 
TATGGTGGAT CAATGTTAGT CCAAAATCAA AAAGACTTGA TCATTTTTAG CGGAACTGTA 
TATCAAGAAA TATTAATATG GGAAATAAAT TATAGTTCTT CATGTACTGA AATTTCACCT 
ATTTTACATC GTTTGCATGG ACATAATGGA GTAATCTTTT CGGTCATATA TGATTCAGTT 
ACTCAACTTA TCTGTTCTAC ATCTGATGAT AGAACAGTCA GATTATGGGA AGTGAATAGC 
AATGAAACCA ATAAAAATCA TATAATTTGG AAAGATGTCA AAATTAAATT AAAAAAAACA 
ATGTTTGGTC ATACAGCAAG AGTATGGAAA TCTATAATAA GAAATGAGAC TTTAATTACC 
ATAGGAGAAG ATTCTCTCAT GTGTACTTGG TCATTAGATG GTAAATTGAT TAATAAAATT 
TGTGCACATC ATGGAGCTGC GATATGGAGC ATTGACATAT CAAGTGATAA CAAAAGCATC 
TTTACTGGTG GAGCTGATGG CGCAGTTCAT GCATGGCCTT TCCCCAGTGA TTATATCCAA 
CCAATTACAC TAGTACCTAG ACAGCATATA TCTATTTTGC CAAAATATAA TTGTTACTTA 
AAAAGTGGTA ATTTCTTAAT TTTTAATGAA AACGGGATTT TGTACATCTA TGATAGGTGG 
CAAAGTAATC CAAAAGAGTT TTTGTATCTT GAAAGGTTTA ATACATATTG CATTATGCAA 
GTTTCACTAT GCCATTCTTA TGTTTGTTTT GCATCAAAAG ATGGATATAT AGCAATATAT 
AAAGAAGCTG ATATTACAAC TAACAAAATA CTACAATTAA TTGTGGAAGA AAAAATAATG 
GAATCACAAA TACTCTCTAT ACAATGGTTA GGAAACAATA ACCTGGTAGC TTGTGGCTTA 
AATGGTGTTC TGAAGATATT CAGTTTTACT ATGGAGGGAT CAGTAACTAT TCAATCAATG 
GGCTTGTTAC CACAATGTCG AGAGCGTTGG TTGACAGCAG CAATATTATA TGAACATGTG 
TTGATATGTG GAGATAGAGC TGGAAATATG CATGTTTTTG AGGTTCAAAA ATATAATTCG 
AATGATGAGA CAAATATACT TAAAATAAAT AACAAACCTA TTCAAACTTT TCCTAAAGTT 
CATGGAAAGA TAGAACCTGA AACCCGTTAC ATGGATGCTG AAATTTATCA TAGTACTACA 
AGCTTACATT GCGTATTGCT TTTCGTTGCA TGCGCAGATG GCTTTGTAAG AATATTTTTG 
TACAATACTA AAACAAAACG TATTTCGTTA AAAATACATG CAAAATGCGC AATTCGTTGC 
ATAGTAAAAA CAACTGTCTT AACATATGAA GAGAAGGTAA TTGCATTAAC TATGTCAACT 
GATGGAATCA TTCGTTTTAT TGATTTCACT GATATCGTTT TAAAAATTAT ACAAGAGCCG 
CATTGTGAGA AGCAAGAATT TCAGAACTAT ATAGATGTAT CTGTTGCGAA ATTTAATTTA 
CATCAATCTG GAATTAATTC ATATGACTTA AAAGCAATTG GAAAAGACGA ATATCTTTTG 
GCAACTGGTG GTGACGACAA TTTATTTAAC ATTATTCGTT TTAAAATTGA AACATCAGGC 
AAAGATCAAC AGTTATACGT TTCATTGTTA TCAAAATGGT GTACGTCCAC TACACATTTT 
GCACAAATTA CAGGAGTAAA ATTTTATGAA GATAAAATTT TTAGTGTTGG AATGGATCAA 
CAAGTCATTA TGTATAATTA TAGCTTTAAT AATGATTGTT TATGTGTAAC AGTTTTAAAG 
AAAATCTTCA CATTTGTAAC AGATGTAAAA GGTCTAACTT TATGTTATAA TTCAAAAGAT 
AAGCCATTTT TGTGTGTTTA TGGAAGAGGA TTTGAAATAT TAACGTATGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100883875 ( XM_012298801.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012154191.1 Megachile rotundata WD repeat-containing protein 6 (LOC100883875), transcript variant X1, mRNA.

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CloneID	OMe98864
Clone ID Related Accession (Same CDS sequence)	XM_012298801.1
Accession Version	XM_012298801.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	WD repeat-containing protein 6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797096.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTCAA AATTGTTATG TACTGATGTG CTTGCAATTC GCTGTGTAAA TAATTTAGTA 
TTTGTAGGTG TGGGTTGTAC ATTACATATT TTTGATATTG ACACTTACAA ATTAGAAAAG 
AAAATCGATT GCCTTTATCC ATACAATATA CATGGAATTA TTGAGGGTCC TAACAATAAG 
ATATCTATAT TTGGGGCTAA CTCTTTATGT GGATATAATA TTCAGAAAAA ACAAGAAACA 
TTAATAGTTA AAGAAGAATT TAGTAAAAAT TGTCTTAATG ATTGGATAAT AACAGTGAAA 
TGGTTATCAT TTGATGGATA TGAGTATTTA GCAGTTTTAT TAGCACATAA TAACGTATGC 
ATTTATGACT GCATCAAGAA AAACTATCAA AATGTATGGT GCAAGGAAAA ATGCATTCTG 
TATGGTGGAT CAATGTTAGT CCAAAATCAA AAAGACTTGA TCATTTTTAG CGGAACTGTA 
TATCAAGAAA TATTAATATG GGAAATAAAT TATAGTTCTT CATGTACTGA AATTTCACCT 
ATTTTACATC GTTTGCATGG ACATAATGGA GTAATCTTTT CGGTCATATA TGATTCAGTT 
ACTCAACTTA TCTGTTCTAC ATCTGATGAT AGAACAGTCA GATTATGGGA AGTGAATAGC 
AATGAAACCA ATAAAAATCA TATAATTTGG AAAGATGTCA AAATTAAATT AAAAAAAACA 
ATGTTTGGTC ATACAGCAAG AGTATGGAAA TCTATAATAA GAAATGAGAC TTTAATTACC 
ATAGGAGAAG ATTCTCTCAT GTGTACTTGG TCATTAGATG GTAAATTGAT TAATAAAATT 
TGTGCACATC ATGGAGCTGC GATATGGAGC ATTGACATAT CAAGTGATAA CAAAAGCATC 
TTTACTGGTG GAGCTGATGG CGCAGTTCAT GCATGGCCTT TCCCCAGTGA TTATATCCAA 
CCAATTACAC TAGTACCTAG ACAGCATATA TCTATTTTGC CAAAATATAA TTGTTACTTA 
AAAAGTGGTA ATTTCTTAAT TTTTAATGAA AACGGGATTT TGTACATCTA TGATAGGTGG 
CAAAGTAATC CAAAAGAGTT TTTGTATCTT GAAAGGTTTA ATACATATTG CATTATGCAA 
GTTTCACTAT GCCATTCTTA TGTTTGTTTT GCATCAAAAG ATGGATATAT AGCAATATAT 
AAAGAAGCTG ATATTACAAC TAACAAAATA CTACAATTAA TTGTGGAAGA AAAAATAATG 
GAATCACAAA TACTCTCTAT ACAATGGTTA GGAAACAATA ACCTGGTAGC TTGTGGCTTA 
AATGGTGTTC TGAAGATATT CAGTTTTACT ATGGAGGGAT CAGTAACTAT TCAATCAATG 
GGCTTGTTAC CACAATGTCG AGAGCGTTGG TTGACAGCAG CAATATTATA TGAACATGTG 
TTGATATGTG GAGATAGAGC TGGAAATATG CATGTTTTTG AGGTTCAAAA ATATAATTCG 
AATGATGAGA CAAATATACT TAAAATAAAT AACAAACCTA TTCAAACTTT TCCTAAAGTT 
CATGGAAAGA TAGGTATTCA AAATTTTATA GTTTTACATT CAAAATTAAT ATCAGGAGGT 
CGTGATGGAA CGTTACGATT TTATGAAATA ACTGAACATC AAAACACAAA ATCTTTATGC 
ACTTTACATT CGGAAAAAAT GCCAATGGAT TGGATTAGTG GCAGTTTAAA AGTATCTGAC 
GACATTTTCA TATTGGGATT CAAAGAAACG GAATTCTTAG TATATAGCAT GTTTTATCGT 
AGAATCGTAA CAAGAGTTCC TTGTGGTGGT GGTCATAGAT CATGGGATTG TATTGTACTA 
AACGAACTTG TTTCATTTCT TTATATTCGT AATAAACAAG TATATATATT TGATTTTCCG 
TTAGCTGCTT TAAAAACCCC GGTTTTATTG AATGGTTTTC ATACAAAAGA AGTAAATTTC 
ATTAACCCTG TATTAAACAC AAACATGCAA AACGTGTTTA TATCTGGAGG AGAAGATGGT 
ACTCTTCATA TTTCTCATGT CTTCAGTACA TTAGTAAAAA ATAATTTGTG CTTACAAACA 
TTAGGTATTT TTGATGGCCA CATTTCCACA GTAAAATTTA TAACTTCCTT AAGTTTGCAA 
TTTAATTCTT CCCGCAATAA ACATCTTGTT TTTTCTGGGG GTGGAAGGGC CCAAATAAAA 
GTGTGGGAAA TTAATATAAA CGATAGCAAA AAATCATTAC AAAGTATAGA TATTTCTTGT 
CATGACGTTA CATCTCATAT GTTGTACGGA TTTGATCGAT ATCGTAAAAA ACAATGTCAG 
GAGTCTAATC GGTCTTACAT CGTAGAACCT GAAACCCGTT ACATGGATGC TGAAATTTAT 
CATAGTACTA CAAGCTTACA TTGCGTATTG CTTTTCGTTG CATGCGCAGA TGGCTTTGTA 
AGAATATTTT TGTACAATAC TAAAACAAAA CGTATTTCGT TAAAAATACA TGCAAAATGC 
GCAATTCGTT GCATAGTAAA AACAACTGTC TTAACATATG AAGAGAAGGT AATTGCATTA 
ACTATGTCAA CTGATGGAAT CATTCGTTTT ATTGATTTCA CTGATATCGT TTTAAAAATT 
ATACAAGAGC CGCATTGTGA GAAGCAAGAA TTTCAGAACT ATATAGATGT ATCTGTTGCG 
AAATTTAATT TACATCAATC TGGAATTAAT TCATATGACT TAAAAGCAAT TGGAAAAGAC 
GAATATCTTT TGGCAACTGG TGGTGACGAC AATTTATTTA ACATTATTCG TTTTAAAATT 
GAAACATCAG GCAAAGATCA ACAGTTATAC GTTTCATTGT TATCAAAATG GTGTACGTCC 
ACTACACATT TTGCACAAAT TACAGGAGTA AAATTTTATG AAGATAAAAT TTTTAGTGTT 
GGAATGGATC AACAAGTCAT TATGTATAAT TATAGCTTTA ATAATGATTG TTTATGTGTA 
ACAGTTTTAA AGAAAATCTT CACATTTGTA ACAGATGTAA AAGGTCTAAC TTTATGTTAT 
AATTCAAAAG ATAAGCCATT TTTGTGTGTT TATGGAAGAG GATTTGAAAT ATTAACGTAT 
GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012154191.1
CDS	596..3721
Translation	MISKLLCTDVLAIRCVNNLVFVGVGCTLHIFDIDTYKLEKKIDCLYPYNIHGIIEGPNNKISIFGANSLCGYNIQKKQETLIVKEEFSKNCLNDWIITVKWLSFDGYEYLAVLLAHNNVCIYDCIKKNYQNVWCKEKCILYGGSMLVQNQKDLIIFSGTVYQEILIWEINYSSSCTEISPILHRLHGHNGVIFSVIYDSVTQLICSTSDDRTVRLWEVNSNETNKNHIIWKDVKIKLKKTMFGHTARVWKSIIRNETLITIGEDSLMCTWSLDGKLINKICAHHGAAIWSIDISSDNKSIFTGGADGAVHAWPFPSDYIQPITLVPRQHISILPKYNCYLKSGNFLIFNENGILYIYDRWQSNPKEFLYLERFNTYCIMQVSLCHSYVCFASKDGYIAIYKEADITTNKILQLIVEEKIMESQILSIQWLGNNNLVACGLNGVLKIFSFTMEGSVTIQSMGLLPQCRERWLTAAILYEHVLICGDRAGNMHVFEVQKYNSNDETNILKINNKPIQTFPKVHGKIGIQNFIVLHSKLISGGRDGTLRFYEITEHQNTKSLCTLHSEKMPMDWISGSLKVSDDIFILGFKETEFLVYSMFYRRIVTRVPCGGGHRSWDCIVLNELVSFLYIRNKQVYIFDFPLAALKTPVLLNGFHTKEVNFINPVLNTNMQNVFISGGEDGTLHISHVFSTLVKNNLCLQTLGIFDGHISTVKFITSLSLQFNSSRNKHLVFSGGGRAQIKVWEININDSKKSLQSIDISCHDVTSHMLYGFDRYRKKQCQESNRSYIVEPETRYMDAEIYHSTTSLHCVLLFVACADGFVRIFLYNTKTKRISLKIHAKCAIRCIVKTTVLTYEEKVIALTMSTDGIIRFIDFTDIVLKIIQEPHCEKQEFQNYIDVSVAKFNLHQSGINSYDLKAIGKDEYLLATGGDDNLFNIIRFKIETSGKDQQLYVSLLSKWCTSTTHFAQITGVKFYEDKIFSVGMDQQVIMYNYSFNNDCLCVTVLKKIFTFVTDVKGLTLCYNSKDKPFLCVYGRGFEILTYE

Target ORF information:

RefSeq Version	XM_012298801.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata WD repeat-containing protein 6 (LOC100883875), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012298801.1

ORF Insert Sequence:

ATGATTTCAA AATTGTTATG TACTGATGTG CTTGCAATTC GCTGTGTAAA TAATTTAGTA 
TTTGTAGGTG TGGGTTGTAC ATTACATATT TTTGATATTG ACACTTACAA ATTAGAAAAG 
AAAATCGATT GCCTTTATCC ATACAATATA CATGGAATTA TTGAGGGTCC TAACAATAAG 
ATATCTATAT TTGGGGCTAA CTCTTTATGT GGATATAATA TTCAGAAAAA ACAAGAAACA 
TTAATAGTTA AAGAAGAATT TAGTAAAAAT TGTCTTAATG ATTGGATAAT AACAGTGAAA 
TGGTTATCAT TTGATGGATA TGAGTATTTA GCAGTTTTAT TAGCACATAA TAACGTATGC 
ATTTATGACT GCATCAAGAA AAACTATCAA AATGTATGGT GCAAGGAAAA ATGCATTCTG 
TATGGTGGAT CAATGTTAGT CCAAAATCAA AAAGACTTGA TCATTTTTAG CGGAACTGTA 
TATCAAGAAA TATTAATATG GGAAATAAAT TATAGTTCTT CATGTACTGA AATTTCACCT 
ATTTTACATC GTTTGCATGG ACATAATGGA GTAATCTTTT CGGTCATATA TGATTCAGTT 
ACTCAACTTA TCTGTTCTAC ATCTGATGAT AGAACAGTCA GATTATGGGA AGTGAATAGC 
AATGAAACCA ATAAAAATCA TATAATTTGG AAAGATGTCA AAATTAAATT AAAAAAAACA 
ATGTTTGGTC ATACAGCAAG AGTATGGAAA TCTATAATAA GAAATGAGAC TTTAATTACC 
ATAGGAGAAG ATTCTCTCAT GTGTACTTGG TCATTAGATG GTAAATTGAT TAATAAAATT 
TGTGCACATC ATGGAGCTGC GATATGGAGC ATTGACATAT CAAGTGATAA CAAAAGCATC 
TTTACTGGTG GAGCTGATGG CGCAGTTCAT GCATGGCCTT TCCCCAGTGA TTATATCCAA 
CCAATTACAC TAGTACCTAG ACAGCATATA TCTATTTTGC CAAAATATAA TTGTTACTTA 
AAAAGTGGTA ATTTCTTAAT TTTTAATGAA AACGGGATTT TGTACATCTA TGATAGGTGG 
CAAAGTAATC CAAAAGAGTT TTTGTATCTT GAAAGGTTTA ATACATATTG CATTATGCAA 
GTTTCACTAT GCCATTCTTA TGTTTGTTTT GCATCAAAAG ATGGATATAT AGCAATATAT 
AAAGAAGCTG ATATTACAAC TAACAAAATA CTACAATTAA TTGTGGAAGA AAAAATAATG 
GAATCACAAA TACTCTCTAT ACAATGGTTA GGAAACAATA ACCTGGTAGC TTGTGGCTTA 
AATGGTGTTC TGAAGATATT CAGTTTTACT ATGGAGGGAT CAGTAACTAT TCAATCAATG 
GGCTTGTTAC CACAATGTCG AGAGCGTTGG TTGACAGCAG CAATATTATA TGAACATGTG 
TTGATATGTG GAGATAGAGC TGGAAATATG CATGTTTTTG AGGTTCAAAA ATATAATTCG 
AATGATGAGA CAAATATACT TAAAATAAAT AACAAACCTA TTCAAACTTT TCCTAAAGTT 
CATGGAAAGA TAGGTATTCA AAATTTTATA GTTTTACATT CAAAATTAAT ATCAGGAGGT 
CGTGATGGAA CGTTACGATT TTATGAAATA ACTGAACATC AAAACACAAA ATCTTTATGC 
ACTTTACATT CGGAAAAAAT GCCAATGGAT TGGATTAGTG GCAGTTTAAA AGTATCTGAC 
GACATTTTCA TATTGGGATT CAAAGAAACG GAATTCTTAG TATATAGCAT GTTTTATCGT 
AGAATCGTAA CAAGAGTTCC TTGTGGTGGT GGTCATAGAT CATGGGATTG TATTGTACTA 
AACGAACTTG TTTCATTTCT TTATATTCGT AATAAACAAG TATATATATT TGATTTTCCG 
TTAGCTGCTT TAAAAACCCC GGTTTTATTG AATGGTTTTC ATACAAAAGA AGTAAATTTC 
ATTAACCCTG TATTAAACAC AAACATGCAA AACGTGTTTA TATCTGGAGG AGAAGATGGT 
ACTCTTCATA TTTCTCATGT CTTCAGTACA TTAGTAAAAA ATAATTTGTG CTTACAAACA 
TTAGGTATTT TTGATGGCCA CATTTCCACA GTAAAATTTA TAACTTCCTT AAGTTTGCAA 
TTTAATTCTT CCCGCAATAA ACATCTTGTT TTTTCTGGGG GTGGAAGGGC CCAAATAAAA 
GTGTGGGAAA TTAATATAAA CGATAGCAAA AAATCATTAC AAAGTATAGA TATTTCTTGT 
CATGACGTTA CATCTCATAT GTTGTACGGA TTTGATCGAT ATCGTAAAAA ACAATGTCAG 
GAGTCTAATC GGTCTTACAT CGTAGAACCT GAAACCCGTT ACATGGATGC TGAAATTTAT 
CATAGTACTA CAAGCTTACA TTGCGTATTG CTTTTCGTTG CATGCGCAGA TGGCTTTGTA 
AGAATATTTT TGTACAATAC TAAAACAAAA CGTATTTCGT TAAAAATACA TGCAAAATGC 
GCAATTCGTT GCATAGTAAA AACAACTGTC TTAACATATG AAGAGAAGGT AATTGCATTA 
ACTATGTCAA CTGATGGAAT CATTCGTTTT ATTGATTTCA CTGATATCGT TTTAAAAATT 
ATACAAGAGC CGCATTGTGA GAAGCAAGAA TTTCAGAACT ATATAGATGT ATCTGTTGCG 
AAATTTAATT TACATCAATC TGGAATTAAT TCATATGACT TAAAAGCAAT TGGAAAAGAC 
GAATATCTTT TGGCAACTGG TGGTGACGAC AATTTATTTA ACATTATTCG TTTTAAAATT 
GAAACATCAG GCAAAGATCA ACAGTTATAC GTTTCATTGT TATCAAAATG GTGTACGTCC 
ACTACACATT TTGCACAAAT TACAGGAGTA AAATTTTATG AAGATAAAAT TTTTAGTGTT 
GGAATGGATC AACAAGTCAT TATGTATAAT TATAGCTTTA ATAATGATTG TTTATGTGTA 
ACAGTTTTAA AGAAAATCTT CACATTTGTA ACAGATGTAA AAGGTCTAAC TTTATGTTAT 
AATTCAAAAG ATAAGCCATT TTTGTGTGTT TATGGAAGAG GATTTGAAAT ATTAACGTAT 
GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC100883875 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム