LOC100883827 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100883827
Entrez Gene ID	100883827
Full Name	nuclear pore complex protein Nup133
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003707740.2	XP_003707788.1	nuclear pore complex protein Nup133
XM_012295903.1	XP_012151293.1	nuclear pore complex protein Nup133

Pathways from BioSystems
Gene Ontology

The following LOC100883827 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100883827 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe113932	XM_003707740.2 Latest version!	Megachile rotundata nuclear pore complex protein Nup133 (LOC100883827), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OMe113932	XM_012295903.1 Latest version!	Megachile rotundata nuclear pore complex protein Nup133 (LOC100883827), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100883827 ( XM_003707740.2 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_003707788.1 Megachile rotundata nuclear pore complex protein Nup133 (LOC100883827), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMe113932
Clone ID Related Accession (Same CDS sequence)	XM_003707740.2 , XM_012295903.1
Accession Version	XM_003707740.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	nuclear pore complex protein Nup133
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797652.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2015 this sequence version replaced XM_003707740.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCGCA CCTTCAAGAA AAACAGTGCA CGCTCCGATC AATCTATACA ACTTCTTACA 
AAGACGAAGT ATCACGCTGT ACAAACTTTT CGCTCGTCGT TACCTGTTCT CGTTACGGAG 
GCTTTGACGT TTCTCGACGA GAATACCACC ACCGTCAGCG TTAATCTTTC ACCCGACGGT 
TGGGCTTGGT TAGTATCGGG ACGCAGATTA CTCGTATGGC GATGCAAGCC TAACGATCCC 
AAACAACGCA CGACTTTCAA CGCCCGATGT CGCGAATTGT CCTTGCCTCA AAGCGATTTG 
GCTCACAAAG CGGAATGTGT CGTTGTATGG CTACAACCGG GGCAACAGCT ACCCAGTTGC 
ATGGCAGTCT CTCCGGAGGG TATTGTCCGC TATTGGCCGA GCGTCGCTCA CGAAGGCTCT 
TCGGTCGAAA CCAGTGCAGA GTTGGCGGGT CAAGAAGTTC ATTGCCTGGC TCGCGTACCT 
GGCCACGGTT GTATCTTGGT CACCACCACC TGTACCGTAG CGTTGTTGCA ACCACGCTTC 
GTCTCCGGCA AGCACGCCGT TAACTGCCAC CTTCTCAGAA CCAGTCAAGG ATGGCTAGGC 
GGTATAGGAC GCAGAATGAC CTCTCTTATT TTCGGAGCTA TACCTCAATC CCCTGTCACC 
GAAACGAAAT TGATCAGGGT CGTTTGCGAC GCTCTCCCCG ACAACGCTTA CAGGGTACTC 
GTTTTGGCCG GTTCCTCCTT GCAGTATTGG TCCTTTCCCT CTAACGAACA GGAAAAAATC 
GAATTCGACG AGGACATCGG ACACCTCGTT TCGCAACTCT TTCAACGACG ATCCAACGAG 
TACGATCCGC AAAATATGGA AACATGGTTG ATCGATATGC AAACCTGCCA AGACGGAATC 
GTCTTTTTAA TGGCAGCCCA TTGTACTACC GGACCGCCTT TCGTTCATCT TGCTCTTGGC 
TCGATACAAT TGTCCGGCAC CACCTTGGCA CGCGACTTCA AATGGTTCCT ACCCCTCAAG 
GAAAATTCTC CCATTTTTTA TCAATCCGAC GTCGAATCCT TTTTAGTTAC CTATCGTTTT 
ATATTGTGCG GATGGGAAGC CATTGTATAC AATCGGCGCG ATGTCCTTGT TGTAAATTAT 
ACGACCGACC ATGAACAAGA CAATATAGAT TTAGGACGAG GCGACAGCAT CCTCGGCGGT 
GCGTCGTTTT CCGGAACTCC GGTTTTGTTC ACCAAAAATT TTGGATTGGT TTCCATCGTT 
CCCACCGATT TTATGTCTCA ACGAGATTTC GATACGACTC ACAACGACAA TACGGAGCAC 
GATCATCGTA TCGATTTAAC TGCGCAAAAT TTTACCGCTC TCATTACCGA CGAACGACTT 
CAAGACATGT ATTACAGTTC CGATACGGCT ACGCGATTAC GCGCTGCCTT TCTTCTCGCT 
CTTCGTCGAG ATAACCAACG ATGCGAGGAA ATTTTGTCTC AACTTTTTCC CTCGCAAGAA 
GAACCGGTCA TGGATATCGA TGCCACTCTC GATACTTTAG TTTTGAAAGT TGCCAAAGAC 
TTGATAGACG ATTATCCTGC CAATGATCCG CGTTGGGCCG ATCGCAGAGA TTTGTCGATT 
ACCCTGAACG CGGTTGCTTC CATGCAGATA CTCAATCAAT TGGAAGGAAA ACTCAAAGCG 
CTGGATTTCT TCGTACGCTT CTTGAAAGAA CACCGTTTGT GGACAAGATT TTGCGCCGTT 
ACCTACAGAG GAATCATTAT GGCTACGCCT CACGTTCTCC AAGAGTACGC CGAAAAATTA 
GTCGCGGCGA TTACCCTCTA CAATCTACAA AACACCTATT CCGATATCGT CGATGCTGTC 
ATCGAACAAA CTTTAACCCC GGATTCCTTC GTGTCCGACC AATTAACCTC TCGCGATATA 
TTTTATCGAC AAGCCAGTAC GGTACATCGT TTTCTTCCTA CCTTGGTAAA CAACGCCACG 
GAAATTACTC GATCCGAAAG ACCGATTCAA CAAGTAGCTC GTTACATCCT GCGAGTCAAT 
GCTATATTAT TGAGTATACT TCGCGAAGTC CTCGAATACC GTCGGCGTAA CGCTAAACGA 
TTTATACCTA CCAGATGTTC CACCGGTATA ACCGAGTATC TTCCGTGGAC AGCGGCCGTT 
GGCAACCACG GATTAAGAAC TTCTCTAACT ACGATGTATA ATTTAACGCT CAAACACGGT 
ATCACCGGTA CCAACGATTC CACCGTTAGA AACGAATTGT ACGAGCAACT GGTCGGTTAC 
ATAGATTTCA TATTGGATGG TCGAAAATCT CACATAGAAA GCGTCAGAGG AACCGAGAAG 
TTCGAGATAC TTATCAAACA ATACGAAACC GATCGAACCA ATCTGATTCA ACCTCTCGTC 
AAGGAGGAAC AATACGACAG CGCCGCGATG CTGGCTGAAA AGTATTGCGA TTTCGCGTCT 
CTCGTACAAA TTTGCGAATT AACCGATAAT AAAAATCGTT TGGACGGATA CATAGAAAAA 
TTTGCTCTTC AAGATTTTGT CGGATTCTTG TTTTCATGGT ACGTCAAAGA TGGACGACAA 
GGCGAATTGG TAGAGAAATG TAGACGCGGC GGTACCCTAG AATTAGCCGA AAAATTAGCG 
GAACACCCTA CTTTGTCCTG GGTACAATCC GCGTTAACCG ACGATTTACG TTTCGCCGCT 
AATACGCTTC ATTCTTTAGC GATACAGGAA AACGAATTAG TCGCTCGTAA AAAGTCTATG 
CTCTCTTTGG CGAAATTGGC TCTCTTGGCT TCGGACGATC CCGAGGAAGA ACTAACGGAT 
CGCATCAAAA GAATTAACAA CGAATTAGCG CTTATAGCTC ATCAAGAAGA TTTACCCACG 
CGAGTGCTTA CCACCTACGG TTACGACGTC GATAAACTAC GAGTATTTAC ACCCACGGAA 
TTAATTACGT TGTACACGTC GGAGGATAAC GTCGACGCGA ACGAGTACGA CTTTAAAAAA 
GCTCTCGATT TATTGGAATA CGTGGAACAA GAGGAAGAAA AGATGTCTTT GAAATTACGA 
ATTTGGGCTA GGGCAGCTAA ACGAGATCGA TGGGATATCG CTGGAAAGAA TCCTGAACAG 
CAAGTGCAAG AAACGATTTT CTTCAAATTA ATGGATCTCG CACGTTTTAT GGGCGGTAAA 
GTCAACGAAT TTCTTCCTCC GGTCGACATG CTTTTGACGG AACCTGAATT GGGAAATCTC 
GCGGCATCCA GCAACTTTCA ATTTCTCATC AAATTCGTCT ACGAGTACGC GTACAGCAAA 
TACTATTTCG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003707788.1
CDS	57..3371
Translation	MDRTFKKNSARSDQSIQLLTKTKYHAVQTFRSSLPVLVTEALTFLDENTTTVSVNLSPDGWAWLVSGRRLLVWRCKPNDPKQRTTFNARCRELSLPQSDLAHKAECVVVWLQPGQQLPSCMAVSPEGIVRYWPSVAHEGSSVETSAELAGQEVHCLARVPGHGCILVTTTCTVALLQPRFVSGKHAVNCHLLRTSQGWLGGIGRRMTSLIFGAIPQSPVTETKLIRVVCDALPDNAYRVLVLAGSSLQYWSFPSNEQEKIEFDEDIGHLVSQLFQRRSNEYDPQNMETWLIDMQTCQDGIVFLMAAHCTTGPPFVHLALGSIQLSGTTLARDFKWFLPLKENSPIFYQSDVESFLVTYRFILCGWEAIVYNRRDVLVVNYTTDHEQDNIDLGRGDSILGGASFSGTPVLFTKNFGLVSIVPTDFMSQRDFDTTHNDNTEHDHRIDLTAQNFTALITDERLQDMYYSSDTATRLRAAFLLALRRDNQRCEEILSQLFPSQEEPVMDIDATLDTLVLKVAKDLIDDYPANDPRWADRRDLSITLNAVASMQILNQLEGKLKALDFFVRFLKEHRLWTRFCAVTYRGIIMATPHVLQEYAEKLVAAITLYNLQNTYSDIVDAVIEQTLTPDSFVSDQLTSRDIFYRQASTVHRFLPTLVNNATEITRSERPIQQVARYILRVNAILLSILREVLEYRRRNAKRFIPTRCSTGITEYLPWTAAVGNHGLRTSLTTMYNLTLKHGITGTNDSTVRNELYEQLVGYIDFILDGRKSHIESVRGTEKFEILIKQYETDRTNLIQPLVKEEQYDSAAMLAEKYCDFASLVQICELTDNKNRLDGYIEKFALQDFVGFLFSWYVKDGRQGELVEKCRRGGTLELAEKLAEHPTLSWVQSALTDDLRFAANTLHSLAIQENELVARKKSMLSLAKLALLASDDPEEELTDRIKRINNELALIAHQEDLPTRVLTTYGYDVDKLRVFTPTELITLYTSEDNVDANEYDFKKALDLLEYVEQEEEKMSLKLRIWARAAKRDRWDIAGKNPEQQVQETIFFKLMDLARFMGGKVNEFLPPVDMLLTEPELGNLAASSNFQFLIKFVYEYAYSKYYFD

Target ORF information:

RefSeq Version	XM_003707740.2
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata nuclear pore complex protein Nup133 (LOC100883827), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003707740.2

ORF Insert Sequence:

ATGGATCGCA CCTTCAAGAA AAACAGTGCA CGCTCCGATC AATCTATACA ACTTCTTACA 
AAGACGAAGT ATCACGCTGT ACAAACTTTT CGCTCGTCGT TACCTGTTCT CGTTACGGAG 
GCTTTGACGT TTCTCGACGA GAATACCACC ACCGTCAGCG TTAATCTTTC ACCCGACGGT 
TGGGCTTGGT TAGTATCGGG ACGCAGATTA CTCGTATGGC GATGCAAGCC TAACGATCCC 
AAACAACGCA CGACTTTCAA CGCCCGATGT CGCGAATTGT CCTTGCCTCA AAGCGATTTG 
GCTCACAAAG CGGAATGTGT CGTTGTATGG CTACAACCGG GGCAACAGCT ACCCAGTTGC 
ATGGCAGTCT CTCCGGAGGG TATTGTCCGC TATTGGCCGA GCGTCGCTCA CGAAGGCTCT 
TCGGTCGAAA CCAGTGCAGA GTTGGCGGGT CAAGAAGTTC ATTGCCTGGC TCGCGTACCT 
GGCCACGGTT GTATCTTGGT CACCACCACC TGTACCGTAG CGTTGTTGCA ACCACGCTTC 
GTCTCCGGCA AGCACGCCGT TAACTGCCAC CTTCTCAGAA CCAGTCAAGG ATGGCTAGGC 
GGTATAGGAC GCAGAATGAC CTCTCTTATT TTCGGAGCTA TACCTCAATC CCCTGTCACC 
GAAACGAAAT TGATCAGGGT CGTTTGCGAC GCTCTCCCCG ACAACGCTTA CAGGGTACTC 
GTTTTGGCCG GTTCCTCCTT GCAGTATTGG TCCTTTCCCT CTAACGAACA GGAAAAAATC 
GAATTCGACG AGGACATCGG ACACCTCGTT TCGCAACTCT TTCAACGACG ATCCAACGAG 
TACGATCCGC AAAATATGGA AACATGGTTG ATCGATATGC AAACCTGCCA AGACGGAATC 
GTCTTTTTAA TGGCAGCCCA TTGTACTACC GGACCGCCTT TCGTTCATCT TGCTCTTGGC 
TCGATACAAT TGTCCGGCAC CACCTTGGCA CGCGACTTCA AATGGTTCCT ACCCCTCAAG 
GAAAATTCTC CCATTTTTTA TCAATCCGAC GTCGAATCCT TTTTAGTTAC CTATCGTTTT 
ATATTGTGCG GATGGGAAGC CATTGTATAC AATCGGCGCG ATGTCCTTGT TGTAAATTAT 
ACGACCGACC ATGAACAAGA CAATATAGAT TTAGGACGAG GCGACAGCAT CCTCGGCGGT 
GCGTCGTTTT CCGGAACTCC GGTTTTGTTC ACCAAAAATT TTGGATTGGT TTCCATCGTT 
CCCACCGATT TTATGTCTCA ACGAGATTTC GATACGACTC ACAACGACAA TACGGAGCAC 
GATCATCGTA TCGATTTAAC TGCGCAAAAT TTTACCGCTC TCATTACCGA CGAACGACTT 
CAAGACATGT ATTACAGTTC CGATACGGCT ACGCGATTAC GCGCTGCCTT TCTTCTCGCT 
CTTCGTCGAG ATAACCAACG ATGCGAGGAA ATTTTGTCTC AACTTTTTCC CTCGCAAGAA 
GAACCGGTCA TGGATATCGA TGCCACTCTC GATACTTTAG TTTTGAAAGT TGCCAAAGAC 
TTGATAGACG ATTATCCTGC CAATGATCCG CGTTGGGCCG ATCGCAGAGA TTTGTCGATT 
ACCCTGAACG CGGTTGCTTC CATGCAGATA CTCAATCAAT TGGAAGGAAA ACTCAAAGCG 
CTGGATTTCT TCGTACGCTT CTTGAAAGAA CACCGTTTGT GGACAAGATT TTGCGCCGTT 
ACCTACAGAG GAATCATTAT GGCTACGCCT CACGTTCTCC AAGAGTACGC CGAAAAATTA 
GTCGCGGCGA TTACCCTCTA CAATCTACAA AACACCTATT CCGATATCGT CGATGCTGTC 
ATCGAACAAA CTTTAACCCC GGATTCCTTC GTGTCCGACC AATTAACCTC TCGCGATATA 
TTTTATCGAC AAGCCAGTAC GGTACATCGT TTTCTTCCTA CCTTGGTAAA CAACGCCACG 
GAAATTACTC GATCCGAAAG ACCGATTCAA CAAGTAGCTC GTTACATCCT GCGAGTCAAT 
GCTATATTAT TGAGTATACT TCGCGAAGTC CTCGAATACC GTCGGCGTAA CGCTAAACGA 
TTTATACCTA CCAGATGTTC CACCGGTATA ACCGAGTATC TTCCGTGGAC AGCGGCCGTT 
GGCAACCACG GATTAAGAAC TTCTCTAACT ACGATGTATA ATTTAACGCT CAAACACGGT 
ATCACCGGTA CCAACGATTC CACCGTTAGA AACGAATTGT ACGAGCAACT GGTCGGTTAC 
ATAGATTTCA TATTGGATGG TCGAAAATCT CACATAGAAA GCGTCAGAGG AACCGAGAAG 
TTCGAGATAC TTATCAAACA ATACGAAACC GATCGAACCA ATCTGATTCA ACCTCTCGTC 
AAGGAGGAAC AATACGACAG CGCCGCGATG CTGGCTGAAA AGTATTGCGA TTTCGCGTCT 
CTCGTACAAA TTTGCGAATT AACCGATAAT AAAAATCGTT TGGACGGATA CATAGAAAAA 
TTTGCTCTTC AAGATTTTGT CGGATTCTTG TTTTCATGGT ACGTCAAAGA TGGACGACAA 
GGCGAATTGG TAGAGAAATG TAGACGCGGC GGTACCCTAG AATTAGCCGA AAAATTAGCG 
GAACACCCTA CTTTGTCCTG GGTACAATCC GCGTTAACCG ACGATTTACG TTTCGCCGCT 
AATACGCTTC ATTCTTTAGC GATACAGGAA AACGAATTAG TCGCTCGTAA AAAGTCTATG 
CTCTCTTTGG CGAAATTGGC TCTCTTGGCT TCGGACGATC CCGAGGAAGA ACTAACGGAT 
CGCATCAAAA GAATTAACAA CGAATTAGCG CTTATAGCTC ATCAAGAAGA TTTACCCACG 
CGAGTGCTTA CCACCTACGG TTACGACGTC GATAAACTAC GAGTATTTAC ACCCACGGAA 
TTAATTACGT TGTACACGTC GGAGGATAAC GTCGACGCGA ACGAGTACGA CTTTAAAAAA 
GCTCTCGATT TATTGGAATA CGTGGAACAA GAGGAAGAAA AGATGTCTTT GAAATTACGA 
ATTTGGGCTA GGGCAGCTAA ACGAGATCGA TGGGATATCG CTGGAAAGAA TCCTGAACAG 
CAAGTGCAAG AAACGATTTT CTTCAAATTA ATGGATCTCG CACGTTTTAT GGGCGGTAAA 
GTCAACGAAT TTCTTCCTCC GGTCGACATG CTTTTGACGG AACCTGAATT GGGAAATCTC 
GCGGCATCCA GCAACTTTCA ATTTCTCATC AAATTCGTCT ACGAGTACGC GTACAGCAAA 
TACTATTTCG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100883827 ( XM_012295903.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012151293.1 Megachile rotundata nuclear pore complex protein Nup133 (LOC100883827), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMe113932
Clone ID Related Accession (Same CDS sequence)	XM_003707740.2 , XM_012295903.1
Accession Version	XM_012295903.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	nuclear pore complex protein Nup133
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797652.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCGCA CCTTCAAGAA AAACAGTGCA CGCTCCGATC AATCTATACA ACTTCTTACA 
AAGACGAAGT ATCACGCTGT ACAAACTTTT CGCTCGTCGT TACCTGTTCT CGTTACGGAG 
GCTTTGACGT TTCTCGACGA GAATACCACC ACCGTCAGCG TTAATCTTTC ACCCGACGGT 
TGGGCTTGGT TAGTATCGGG ACGCAGATTA CTCGTATGGC GATGCAAGCC TAACGATCCC 
AAACAACGCA CGACTTTCAA CGCCCGATGT CGCGAATTGT CCTTGCCTCA AAGCGATTTG 
GCTCACAAAG CGGAATGTGT CGTTGTATGG CTACAACCGG GGCAACAGCT ACCCAGTTGC 
ATGGCAGTCT CTCCGGAGGG TATTGTCCGC TATTGGCCGA GCGTCGCTCA CGAAGGCTCT 
TCGGTCGAAA CCAGTGCAGA GTTGGCGGGT CAAGAAGTTC ATTGCCTGGC TCGCGTACCT 
GGCCACGGTT GTATCTTGGT CACCACCACC TGTACCGTAG CGTTGTTGCA ACCACGCTTC 
GTCTCCGGCA AGCACGCCGT TAACTGCCAC CTTCTCAGAA CCAGTCAAGG ATGGCTAGGC 
GGTATAGGAC GCAGAATGAC CTCTCTTATT TTCGGAGCTA TACCTCAATC CCCTGTCACC 
GAAACGAAAT TGATCAGGGT CGTTTGCGAC GCTCTCCCCG ACAACGCTTA CAGGGTACTC 
GTTTTGGCCG GTTCCTCCTT GCAGTATTGG TCCTTTCCCT CTAACGAACA GGAAAAAATC 
GAATTCGACG AGGACATCGG ACACCTCGTT TCGCAACTCT TTCAACGACG ATCCAACGAG 
TACGATCCGC AAAATATGGA AACATGGTTG ATCGATATGC AAACCTGCCA AGACGGAATC 
GTCTTTTTAA TGGCAGCCCA TTGTACTACC GGACCGCCTT TCGTTCATCT TGCTCTTGGC 
TCGATACAAT TGTCCGGCAC CACCTTGGCA CGCGACTTCA AATGGTTCCT ACCCCTCAAG 
GAAAATTCTC CCATTTTTTA TCAATCCGAC GTCGAATCCT TTTTAGTTAC CTATCGTTTT 
ATATTGTGCG GATGGGAAGC CATTGTATAC AATCGGCGCG ATGTCCTTGT TGTAAATTAT 
ACGACCGACC ATGAACAAGA CAATATAGAT TTAGGACGAG GCGACAGCAT CCTCGGCGGT 
GCGTCGTTTT CCGGAACTCC GGTTTTGTTC ACCAAAAATT TTGGATTGGT TTCCATCGTT 
CCCACCGATT TTATGTCTCA ACGAGATTTC GATACGACTC ACAACGACAA TACGGAGCAC 
GATCATCGTA TCGATTTAAC TGCGCAAAAT TTTACCGCTC TCATTACCGA CGAACGACTT 
CAAGACATGT ATTACAGTTC CGATACGGCT ACGCGATTAC GCGCTGCCTT TCTTCTCGCT 
CTTCGTCGAG ATAACCAACG ATGCGAGGAA ATTTTGTCTC AACTTTTTCC CTCGCAAGAA 
GAACCGGTCA TGGATATCGA TGCCACTCTC GATACTTTAG TTTTGAAAGT TGCCAAAGAC 
TTGATAGACG ATTATCCTGC CAATGATCCG CGTTGGGCCG ATCGCAGAGA TTTGTCGATT 
ACCCTGAACG CGGTTGCTTC CATGCAGATA CTCAATCAAT TGGAAGGAAA ACTCAAAGCG 
CTGGATTTCT TCGTACGCTT CTTGAAAGAA CACCGTTTGT GGACAAGATT TTGCGCCGTT 
ACCTACAGAG GAATCATTAT GGCTACGCCT CACGTTCTCC AAGAGTACGC CGAAAAATTA 
GTCGCGGCGA TTACCCTCTA CAATCTACAA AACACCTATT CCGATATCGT CGATGCTGTC 
ATCGAACAAA CTTTAACCCC GGATTCCTTC GTGTCCGACC AATTAACCTC TCGCGATATA 
TTTTATCGAC AAGCCAGTAC GGTACATCGT TTTCTTCCTA CCTTGGTAAA CAACGCCACG 
GAAATTACTC GATCCGAAAG ACCGATTCAA CAAGTAGCTC GTTACATCCT GCGAGTCAAT 
GCTATATTAT TGAGTATACT TCGCGAAGTC CTCGAATACC GTCGGCGTAA CGCTAAACGA 
TTTATACCTA CCAGATGTTC CACCGGTATA ACCGAGTATC TTCCGTGGAC AGCGGCCGTT 
GGCAACCACG GATTAAGAAC TTCTCTAACT ACGATGTATA ATTTAACGCT CAAACACGGT 
ATCACCGGTA CCAACGATTC CACCGTTAGA AACGAATTGT ACGAGCAACT GGTCGGTTAC 
ATAGATTTCA TATTGGATGG TCGAAAATCT CACATAGAAA GCGTCAGAGG AACCGAGAAG 
TTCGAGATAC TTATCAAACA ATACGAAACC GATCGAACCA ATCTGATTCA ACCTCTCGTC 
AAGGAGGAAC AATACGACAG CGCCGCGATG CTGGCTGAAA AGTATTGCGA TTTCGCGTCT 
CTCGTACAAA TTTGCGAATT AACCGATAAT AAAAATCGTT TGGACGGATA CATAGAAAAA 
TTTGCTCTTC AAGATTTTGT CGGATTCTTG TTTTCATGGT ACGTCAAAGA TGGACGACAA 
GGCGAATTGG TAGAGAAATG TAGACGCGGC GGTACCCTAG AATTAGCCGA AAAATTAGCG 
GAACACCCTA CTTTGTCCTG GGTACAATCC GCGTTAACCG ACGATTTACG TTTCGCCGCT 
AATACGCTTC ATTCTTTAGC GATACAGGAA AACGAATTAG TCGCTCGTAA AAAGTCTATG 
CTCTCTTTGG CGAAATTGGC TCTCTTGGCT TCGGACGATC CCGAGGAAGA ACTAACGGAT 
CGCATCAAAA GAATTAACAA CGAATTAGCG CTTATAGCTC ATCAAGAAGA TTTACCCACG 
CGAGTGCTTA CCACCTACGG TTACGACGTC GATAAACTAC GAGTATTTAC ACCCACGGAA 
TTAATTACGT TGTACACGTC GGAGGATAAC GTCGACGCGA ACGAGTACGA CTTTAAAAAA 
GCTCTCGATT TATTGGAATA CGTGGAACAA GAGGAAGAAA AGATGTCTTT GAAATTACGA 
ATTTGGGCTA GGGCAGCTAA ACGAGATCGA TGGGATATCG CTGGAAAGAA TCCTGAACAG 
CAAGTGCAAG AAACGATTTT CTTCAAATTA ATGGATCTCG CACGTTTTAT GGGCGGTAAA 
GTCAACGAAT TTCTTCCTCC GGTCGACATG CTTTTGACGG AACCTGAATT GGGAAATCTC 
GCGGCATCCA GCAACTTTCA ATTTCTCATC AAATTCGTCT ACGAGTACGC GTACAGCAAA 
TACTATTTCG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012151293.1
CDS	57..3371
Translation	MDRTFKKNSARSDQSIQLLTKTKYHAVQTFRSSLPVLVTEALTFLDENTTTVSVNLSPDGWAWLVSGRRLLVWRCKPNDPKQRTTFNARCRELSLPQSDLAHKAECVVVWLQPGQQLPSCMAVSPEGIVRYWPSVAHEGSSVETSAELAGQEVHCLARVPGHGCILVTTTCTVALLQPRFVSGKHAVNCHLLRTSQGWLGGIGRRMTSLIFGAIPQSPVTETKLIRVVCDALPDNAYRVLVLAGSSLQYWSFPSNEQEKIEFDEDIGHLVSQLFQRRSNEYDPQNMETWLIDMQTCQDGIVFLMAAHCTTGPPFVHLALGSIQLSGTTLARDFKWFLPLKENSPIFYQSDVESFLVTYRFILCGWEAIVYNRRDVLVVNYTTDHEQDNIDLGRGDSILGGASFSGTPVLFTKNFGLVSIVPTDFMSQRDFDTTHNDNTEHDHRIDLTAQNFTALITDERLQDMYYSSDTATRLRAAFLLALRRDNQRCEEILSQLFPSQEEPVMDIDATLDTLVLKVAKDLIDDYPANDPRWADRRDLSITLNAVASMQILNQLEGKLKALDFFVRFLKEHRLWTRFCAVTYRGIIMATPHVLQEYAEKLVAAITLYNLQNTYSDIVDAVIEQTLTPDSFVSDQLTSRDIFYRQASTVHRFLPTLVNNATEITRSERPIQQVARYILRVNAILLSILREVLEYRRRNAKRFIPTRCSTGITEYLPWTAAVGNHGLRTSLTTMYNLTLKHGITGTNDSTVRNELYEQLVGYIDFILDGRKSHIESVRGTEKFEILIKQYETDRTNLIQPLVKEEQYDSAAMLAEKYCDFASLVQICELTDNKNRLDGYIEKFALQDFVGFLFSWYVKDGRQGELVEKCRRGGTLELAEKLAEHPTLSWVQSALTDDLRFAANTLHSLAIQENELVARKKSMLSLAKLALLASDDPEEELTDRIKRINNELALIAHQEDLPTRVLTTYGYDVDKLRVFTPTELITLYTSEDNVDANEYDFKKALDLLEYVEQEEEKMSLKLRIWARAAKRDRWDIAGKNPEQQVQETIFFKLMDLARFMGGKVNEFLPPVDMLLTEPELGNLAASSNFQFLIKFVYEYAYSKYYFD

Target ORF information:

RefSeq Version	XM_012295903.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata nuclear pore complex protein Nup133 (LOC100883827), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012295903.1

ORF Insert Sequence:

ATGGATCGCA CCTTCAAGAA AAACAGTGCA CGCTCCGATC AATCTATACA ACTTCTTACA 
AAGACGAAGT ATCACGCTGT ACAAACTTTT CGCTCGTCGT TACCTGTTCT CGTTACGGAG 
GCTTTGACGT TTCTCGACGA GAATACCACC ACCGTCAGCG TTAATCTTTC ACCCGACGGT 
TGGGCTTGGT TAGTATCGGG ACGCAGATTA CTCGTATGGC GATGCAAGCC TAACGATCCC 
AAACAACGCA CGACTTTCAA CGCCCGATGT CGCGAATTGT CCTTGCCTCA AAGCGATTTG 
GCTCACAAAG CGGAATGTGT CGTTGTATGG CTACAACCGG GGCAACAGCT ACCCAGTTGC 
ATGGCAGTCT CTCCGGAGGG TATTGTCCGC TATTGGCCGA GCGTCGCTCA CGAAGGCTCT 
TCGGTCGAAA CCAGTGCAGA GTTGGCGGGT CAAGAAGTTC ATTGCCTGGC TCGCGTACCT 
GGCCACGGTT GTATCTTGGT CACCACCACC TGTACCGTAG CGTTGTTGCA ACCACGCTTC 
GTCTCCGGCA AGCACGCCGT TAACTGCCAC CTTCTCAGAA CCAGTCAAGG ATGGCTAGGC 
GGTATAGGAC GCAGAATGAC CTCTCTTATT TTCGGAGCTA TACCTCAATC CCCTGTCACC 
GAAACGAAAT TGATCAGGGT CGTTTGCGAC GCTCTCCCCG ACAACGCTTA CAGGGTACTC 
GTTTTGGCCG GTTCCTCCTT GCAGTATTGG TCCTTTCCCT CTAACGAACA GGAAAAAATC 
GAATTCGACG AGGACATCGG ACACCTCGTT TCGCAACTCT TTCAACGACG ATCCAACGAG 
TACGATCCGC AAAATATGGA AACATGGTTG ATCGATATGC AAACCTGCCA AGACGGAATC 
GTCTTTTTAA TGGCAGCCCA TTGTACTACC GGACCGCCTT TCGTTCATCT TGCTCTTGGC 
TCGATACAAT TGTCCGGCAC CACCTTGGCA CGCGACTTCA AATGGTTCCT ACCCCTCAAG 
GAAAATTCTC CCATTTTTTA TCAATCCGAC GTCGAATCCT TTTTAGTTAC CTATCGTTTT 
ATATTGTGCG GATGGGAAGC CATTGTATAC AATCGGCGCG ATGTCCTTGT TGTAAATTAT 
ACGACCGACC ATGAACAAGA CAATATAGAT TTAGGACGAG GCGACAGCAT CCTCGGCGGT 
GCGTCGTTTT CCGGAACTCC GGTTTTGTTC ACCAAAAATT TTGGATTGGT TTCCATCGTT 
CCCACCGATT TTATGTCTCA ACGAGATTTC GATACGACTC ACAACGACAA TACGGAGCAC 
GATCATCGTA TCGATTTAAC TGCGCAAAAT TTTACCGCTC TCATTACCGA CGAACGACTT 
CAAGACATGT ATTACAGTTC CGATACGGCT ACGCGATTAC GCGCTGCCTT TCTTCTCGCT 
CTTCGTCGAG ATAACCAACG ATGCGAGGAA ATTTTGTCTC AACTTTTTCC CTCGCAAGAA 
GAACCGGTCA TGGATATCGA TGCCACTCTC GATACTTTAG TTTTGAAAGT TGCCAAAGAC 
TTGATAGACG ATTATCCTGC CAATGATCCG CGTTGGGCCG ATCGCAGAGA TTTGTCGATT 
ACCCTGAACG CGGTTGCTTC CATGCAGATA CTCAATCAAT TGGAAGGAAA ACTCAAAGCG 
CTGGATTTCT TCGTACGCTT CTTGAAAGAA CACCGTTTGT GGACAAGATT TTGCGCCGTT 
ACCTACAGAG GAATCATTAT GGCTACGCCT CACGTTCTCC AAGAGTACGC CGAAAAATTA 
GTCGCGGCGA TTACCCTCTA CAATCTACAA AACACCTATT CCGATATCGT CGATGCTGTC 
ATCGAACAAA CTTTAACCCC GGATTCCTTC GTGTCCGACC AATTAACCTC TCGCGATATA 
TTTTATCGAC AAGCCAGTAC GGTACATCGT TTTCTTCCTA CCTTGGTAAA CAACGCCACG 
GAAATTACTC GATCCGAAAG ACCGATTCAA CAAGTAGCTC GTTACATCCT GCGAGTCAAT 
GCTATATTAT TGAGTATACT TCGCGAAGTC CTCGAATACC GTCGGCGTAA CGCTAAACGA 
TTTATACCTA CCAGATGTTC CACCGGTATA ACCGAGTATC TTCCGTGGAC AGCGGCCGTT 
GGCAACCACG GATTAAGAAC TTCTCTAACT ACGATGTATA ATTTAACGCT CAAACACGGT 
ATCACCGGTA CCAACGATTC CACCGTTAGA AACGAATTGT ACGAGCAACT GGTCGGTTAC 
ATAGATTTCA TATTGGATGG TCGAAAATCT CACATAGAAA GCGTCAGAGG AACCGAGAAG 
TTCGAGATAC TTATCAAACA ATACGAAACC GATCGAACCA ATCTGATTCA ACCTCTCGTC 
AAGGAGGAAC AATACGACAG CGCCGCGATG CTGGCTGAAA AGTATTGCGA TTTCGCGTCT 
CTCGTACAAA TTTGCGAATT AACCGATAAT AAAAATCGTT TGGACGGATA CATAGAAAAA 
TTTGCTCTTC AAGATTTTGT CGGATTCTTG TTTTCATGGT ACGTCAAAGA TGGACGACAA 
GGCGAATTGG TAGAGAAATG TAGACGCGGC GGTACCCTAG AATTAGCCGA AAAATTAGCG 
GAACACCCTA CTTTGTCCTG GGTACAATCC GCGTTAACCG ACGATTTACG TTTCGCCGCT 
AATACGCTTC ATTCTTTAGC GATACAGGAA AACGAATTAG TCGCTCGTAA AAAGTCTATG 
CTCTCTTTGG CGAAATTGGC TCTCTTGGCT TCGGACGATC CCGAGGAAGA ACTAACGGAT 
CGCATCAAAA GAATTAACAA CGAATTAGCG CTTATAGCTC ATCAAGAAGA TTTACCCACG 
CGAGTGCTTA CCACCTACGG TTACGACGTC GATAAACTAC GAGTATTTAC ACCCACGGAA 
TTAATTACGT TGTACACGTC GGAGGATAAC GTCGACGCGA ACGAGTACGA CTTTAAAAAA 
GCTCTCGATT TATTGGAATA CGTGGAACAA GAGGAAGAAA AGATGTCTTT GAAATTACGA 
ATTTGGGCTA GGGCAGCTAA ACGAGATCGA TGGGATATCG CTGGAAAGAA TCCTGAACAG 
CAAGTGCAAG AAACGATTTT CTTCAAATTA ATGGATCTCG CACGTTTTAT GGGCGGTAAA 
GTCAACGAAT TTCTTCCTCC GGTCGACATG CTTTTGACGG AACCTGAATT GGGAAATCTC 
GCGGCATCCA GCAACTTTCA ATTTCTCATC AAATTCGTCT ACGAGTACGC GTACAGCAAA 
TACTATTTCG ACTGA