LOC100883271 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100883271
Entrez Gene ID	100883271
Full Name	serine/threonine-protein kinase Nek3
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003699703.2	XP_003699751.1	serine/threonine-protein kinase Nek3 isoform X2
XM_012288280.1	XP_012143670.1	serine/threonine-protein kinase Nek3 isoform X2
XM_012288271.1	XP_012143661.1	hormonally up-regulated neu tumor-associated kinase homolog A isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC100883271 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100883271 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMe97429	XM_012288271.1 Latest version!	Megachile rotundata serine/threonine-protein kinase Nek3 (LOC100883271), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OMe97430	XM_003699703.2 Latest version!	Megachile rotundata serine/threonine-protein kinase Nek3 (LOC100883271), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OMe97430	XM_012288280.1 Latest version!	Megachile rotundata serine/threonine-protein kinase Nek3 (LOC100883271), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100883271 ( XM_012288271.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012143661.1 Megachile rotundata serine/threonine-protein kinase Nek3 (LOC100883271), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMe97429
Clone ID Related Accession (Same CDS sequence)	XM_012288271.1
Accession Version	XM_012288271.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2379bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	hormonally up-regulated neu tumor-associated kinase homolog A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797077.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCCC GTGTCGTTCA CACTGGCTCT CCGCGATTCA CGGAGAAAAA TCGAGGGCGC 
AGCTGCTCGA TCCATCGCAT CACGACTAAC GATCCCGTGA CCGAGACAAC AGCTGAAACG 
TGCTCTCTTA CCTTCTTCGA ACAGGTGAAT CTGATCGACG AGTTCGATAT CCTGCAAATC 
GTTGGCGAGG GATGGTTCGG CAAGATCCTG CTTACGGAGC ATCGCAGCAC CCAGACAGAG 
ATGGTGCTGA AAGCGCTGCC GAAGGCTTAC ACCGGCATCT CGGACTTCTT TCGGGAATTC 
CATTATGGGC TACACCTGGC TGCGCACAGG AACATCATCA CCACCTACGA TGTGGCCTTC 
GAGACCGCTG GCTTCTACGT TTTCAGTCAG GAGTACGCTC CTCTCGGAGA TCTAACGTCG 
AACGTGACAG AGACCGGTTT AGGCGAGCTC CACGCGAAGA GGGTTGCCAG ACAACTGGCT 
GCAGCGGTGC ATCACATTCA CAGCCGCGAA TTGGTGCATC GTGACATCAA ACTTGACAAC 
ATCCTCGTGT TCAAGAGTGA CTTCTCGCGA ATCAAATTGT GCGACTTCGG CGAGACCAGA 
CGAGTCAACA CTGTAGTCCG AAGGCACAAC GAGTGGCTGC CATACTCGCC GCCTGAGGTA 
TTGCAGATCG ACACTGACGA GACGTACAAA GCTCTCACAT CGCACGATGT CTGGCAATTC 
GGCATTGTCC TATTTGTCTG TCTGACCGGA TGCTTGCCGT GGCAGAAGGC GGCGTTGGAC 
GACCCGCGTT ACACCAGATA TCAAAACTGG CACAACGCGA CGCTGAACAT AGCCAAGAAA 
CCGAAATTAT TTCAGCTGAT CAGCTCACGG GCGCAGAGGT TGTTCAAGCG TCTGCTGGAC 
CCGAAGGCCG AGAAGAGGCC TGTCAGCGTG CTGGAGGTAA ACAAATACCT GGAGGACAGG 
TGGCTGGCGA AACTGGGTGC GGAGAAGGCC ATGAACGGTG GCGCCGACGA GAGGGACGAG 
CTATGTCCCT CGATGTACAG CTTCCACAGT TCGTTGGAGG AGAAGAATCA GCTCCTTCAC 
ACCCTGACCA CGTACGGAAT CGAGACTACG GTTGACAGAG CGAAGAAGAA AGACCGTATC 
AGAGAGTGGA TTCAAGCGAG CGCGATAGTA GAAGAGTACG AAGAGGACGA GTCAGACCTG 
AACGAGGACA TCCGCTACTA CGAGGACGAG GACGAAGAGC CGAGTTCGAT GAAGGGTAGA 
CACTTCCCGG AGACCCGTCC AATCGCCAAG AACGAGTCCA AACGACACAG ACACAGAAGC 
AACACCTCTA ACCCAAGAAA GCGACAAATC ATCAAACCCA CCGAGAGGAA GATCCCGTTC 
GCTCCTCAAG TGGCCGAAGA GCGACAGACG ATCGCCCGAT TCGCCAGTTT CCCGAACGGC 
GACCCGAACC TGGCGGTGCT GAAGAATGGA ACCAACAGCG ACCCGGTCCT CCCGTTGATG 
ATCGAATCCA ACACGGAGGA GACGAGGTCC GTTAACAACG GCCAGGCCGA CAGCGCCTCT 
CGAGAGGACT CGTTACCGAC GAGTAGTTTC AAACCGAACT TGCCCATCCA GGAGCCGGAG 
AACACGAACG CTCAAGATCA GAACTCGCAA ACGGATCGCA GTTTGCCGAG AAGCCCGCAG 
ATCCCGACCA GGAAGCATCG CTCGCAAACT GCCCCGTTTC CGGCGCCGAC GTCGCCCGTG 
CTGAACGGAA ACCGGGCCGA GTCCGCGAAG AGGAGTCAGT CGACCACCGT GCTGCCCACG 
GACAGATCGA GCCATCGATC GATGCCGAGC CAGTCGAATG GCCAGCCCTC GGTGCCTCGG 
GGCGAAAGGT CGACCACGCA GCCGGAGAAT CGATCCGCGG CGCAATCAAT GTCTCGAGTA 
GAAAATCAAT CCGCGGTAAT GCTGCCGGCG ACGGCTCGAG TGGAGAATCA ATCGAGTAAC 
GCTGTGCAGC TGGCCAGGAA CGAGAAGTCC GCGAGCGGGC TAGTTCCCCT CGCGGCCGCA 
AATCCGCTGA CGATGTCGGT CGCCTCTCAT CTGGTGATGC AGAGCTTCAA CGCGTTGCAG 
GGGATCAACG CGGACGCTAA CCACGCCGCC AGCCCGAAAT CGCCCAGAAA CAGTCCCAAT 
TCTAACCACA GCAAGATGTC CACGCCGAAG AACAGCCCCA CGACTACGCC CGCGAGAACC 
TCCACGCCAC CGAAACACAA AATGAATCAG CGGACTGAAG AGACGGTCTT CTACGGAAAG 
GATCCTTTCG AGCACTACGG TATCGCTCAG GGGGCGATCC TGAGGGGCGA TGCCCGACGA 
GGCTCGCTCG AGAGCGCCAG CAGATCTGTC AGCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012143661.1
CDS	213..2591
Translation	MDSRVVHTGSPRFTEKNRGRSCSIHRITTNDPVTETTAETCSLTFFEQVNLIDEFDILQIVGEGWFGKILLTEHRSTQTEMVLKALPKAYTGISDFFREFHYGLHLAAHRNIITTYDVAFETAGFYVFSQEYAPLGDLTSNVTETGLGELHAKRVARQLAAAVHHIHSRELVHRDIKLDNILVFKSDFSRIKLCDFGETRRVNTVVRRHNEWLPYSPPEVLQIDTDETYKALTSHDVWQFGIVLFVCLTGCLPWQKAALDDPRYTRYQNWHNATLNIAKKPKLFQLISSRAQRLFKRLLDPKAEKRPVSVLEVNKYLEDRWLAKLGAEKAMNGGADERDELCPSMYSFHSSLEEKNQLLHTLTTYGIETTVDRAKKKDRIREWIQASAIVEEYEEDESDLNEDIRYYEDEDEEPSSMKGRHFPETRPIAKNESKRHRHRSNTSNPRKRQIIKPTERKIPFAPQVAEERQTIARFASFPNGDPNLAVLKNGTNSDPVLPLMIESNTEETRSVNNGQADSASREDSLPTSSFKPNLPIQEPENTNAQDQNSQTDRSLPRSPQIPTRKHRSQTAPFPAPTSPVLNGNRAESAKRSQSTTVLPTDRSSHRSMPSQSNGQPSVPRGERSTTQPENRSAAQSMSRVENQSAVMLPATARVENQSSNAVQLARNEKSASGLVPLAAANPLTMSVASHLVMQSFNALQGINADANHAASPKSPRNSPNSNHSKMSTPKNSPTTTPARTSTPPKHKMNQRTEETVFYGKDPFEHYGIAQGAILRGDARRGSLESASRSVSN

Target ORF information:

RefSeq Version	XM_012288271.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata serine/threonine-protein kinase Nek3 (LOC100883271), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012288271.1

ORF Insert Sequence:

ATGGACTCCC GTGTCGTTCA CACTGGCTCT CCGCGATTCA CGGAGAAAAA TCGAGGGCGC 
AGCTGCTCGA TCCATCGCAT CACGACTAAC GATCCCGTGA CCGAGACAAC AGCTGAAACG 
TGCTCTCTTA CCTTCTTCGA ACAGGTGAAT CTGATCGACG AGTTCGATAT CCTGCAAATC 
GTTGGCGAGG GATGGTTCGG CAAGATCCTG CTTACGGAGC ATCGCAGCAC CCAGACAGAG 
ATGGTGCTGA AAGCGCTGCC GAAGGCTTAC ACCGGCATCT CGGACTTCTT TCGGGAATTC 
CATTATGGGC TACACCTGGC TGCGCACAGG AACATCATCA CCACCTACGA TGTGGCCTTC 
GAGACCGCTG GCTTCTACGT TTTCAGTCAG GAGTACGCTC CTCTCGGAGA TCTAACGTCG 
AACGTGACAG AGACCGGTTT AGGCGAGCTC CACGCGAAGA GGGTTGCCAG ACAACTGGCT 
GCAGCGGTGC ATCACATTCA CAGCCGCGAA TTGGTGCATC GTGACATCAA ACTTGACAAC 
ATCCTCGTGT TCAAGAGTGA CTTCTCGCGA ATCAAATTGT GCGACTTCGG CGAGACCAGA 
CGAGTCAACA CTGTAGTCCG AAGGCACAAC GAGTGGCTGC CATACTCGCC GCCTGAGGTA 
TTGCAGATCG ACACTGACGA GACGTACAAA GCTCTCACAT CGCACGATGT CTGGCAATTC 
GGCATTGTCC TATTTGTCTG TCTGACCGGA TGCTTGCCGT GGCAGAAGGC GGCGTTGGAC 
GACCCGCGTT ACACCAGATA TCAAAACTGG CACAACGCGA CGCTGAACAT AGCCAAGAAA 
CCGAAATTAT TTCAGCTGAT CAGCTCACGG GCGCAGAGGT TGTTCAAGCG TCTGCTGGAC 
CCGAAGGCCG AGAAGAGGCC TGTCAGCGTG CTGGAGGTAA ACAAATACCT GGAGGACAGG 
TGGCTGGCGA AACTGGGTGC GGAGAAGGCC ATGAACGGTG GCGCCGACGA GAGGGACGAG 
CTATGTCCCT CGATGTACAG CTTCCACAGT TCGTTGGAGG AGAAGAATCA GCTCCTTCAC 
ACCCTGACCA CGTACGGAAT CGAGACTACG GTTGACAGAG CGAAGAAGAA AGACCGTATC 
AGAGAGTGGA TTCAAGCGAG CGCGATAGTA GAAGAGTACG AAGAGGACGA GTCAGACCTG 
AACGAGGACA TCCGCTACTA CGAGGACGAG GACGAAGAGC CGAGTTCGAT GAAGGGTAGA 
CACTTCCCGG AGACCCGTCC AATCGCCAAG AACGAGTCCA AACGACACAG ACACAGAAGC 
AACACCTCTA ACCCAAGAAA GCGACAAATC ATCAAACCCA CCGAGAGGAA GATCCCGTTC 
GCTCCTCAAG TGGCCGAAGA GCGACAGACG ATCGCCCGAT TCGCCAGTTT CCCGAACGGC 
GACCCGAACC TGGCGGTGCT GAAGAATGGA ACCAACAGCG ACCCGGTCCT CCCGTTGATG 
ATCGAATCCA ACACGGAGGA GACGAGGTCC GTTAACAACG GCCAGGCCGA CAGCGCCTCT 
CGAGAGGACT CGTTACCGAC GAGTAGTTTC AAACCGAACT TGCCCATCCA GGAGCCGGAG 
AACACGAACG CTCAAGATCA GAACTCGCAA ACGGATCGCA GTTTGCCGAG AAGCCCGCAG 
ATCCCGACCA GGAAGCATCG CTCGCAAACT GCCCCGTTTC CGGCGCCGAC GTCGCCCGTG 
CTGAACGGAA ACCGGGCCGA GTCCGCGAAG AGGAGTCAGT CGACCACCGT GCTGCCCACG 
GACAGATCGA GCCATCGATC GATGCCGAGC CAGTCGAATG GCCAGCCCTC GGTGCCTCGG 
GGCGAAAGGT CGACCACGCA GCCGGAGAAT CGATCCGCGG CGCAATCAAT GTCTCGAGTA 
GAAAATCAAT CCGCGGTAAT GCTGCCGGCG ACGGCTCGAG TGGAGAATCA ATCGAGTAAC 
GCTGTGCAGC TGGCCAGGAA CGAGAAGTCC GCGAGCGGGC TAGTTCCCCT CGCGGCCGCA 
AATCCGCTGA CGATGTCGGT CGCCTCTCAT CTGGTGATGC AGAGCTTCAA CGCGTTGCAG 
GGGATCAACG CGGACGCTAA CCACGCCGCC AGCCCGAAAT CGCCCAGAAA CAGTCCCAAT 
TCTAACCACA GCAAGATGTC CACGCCGAAG AACAGCCCCA CGACTACGCC CGCGAGAACC 
TCCACGCCAC CGAAACACAA AATGAATCAG CGGACTGAAG AGACGGTCTT CTACGGAAAG 
GATCCTTTCG AGCACTACGG TATCGCTCAG GGGGCGATCC TGAGGGGCGA TGCCCGACGA 
GGCTCGCTCG AGAGCGCCAG CAGATCTGTC AGCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100883271 ( XM_003699703.2 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_003699751.1 Megachile rotundata serine/threonine-protein kinase Nek3 (LOC100883271), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMe97430
Clone ID Related Accession (Same CDS sequence)	XM_003699703.2 , XM_012288280.1
Accession Version	XM_003699703.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	serine/threonine-protein kinase Nek3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797077.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2015 this sequence version replaced XM_003699703.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTAA TGAAGAAGGC GATCGGCGGT TCCATTCACA GGATACGCGA ATTCGAGCTC 
GAGAAGGTGA ATCTGATCGA CGAGTTCGAT ATCCTGCAAA TCGTTGGCGA GGGATGGTTC 
GGCAAGATCC TGCTTACGGA GCATCGCAGC ACCCAGACAG AGATGGTGCT GAAAGCGCTG 
CCGAAGGCTT ACACCGGCAT CTCGGACTTC TTTCGGGAAT TCCATTATGG GCTACACCTG 
GCTGCGCACA GGAACATCAT CACCACCTAC GATGTGGCCT TCGAGACCGC TGGCTTCTAC 
GTTTTCAGTC AGGAGTACGC TCCTCTCGGA GATCTAACGT CGAACGTGAC AGAGACCGGT 
TTAGGCGAGC TCCACGCGAA GAGGGTTGCC AGACAACTGG CTGCAGCGGT GCATCACATT 
CACAGCCGCG AATTGGTGCA TCGTGACATC AAACTTGACA ACATCCTCGT GTTCAAGAGT 
GACTTCTCGC GAATCAAATT GTGCGACTTC GGCGAGACCA GACGAGTCAA CACTGTAGTC 
CGAAGGCACA ACGAGTGGCT GCCATACTCG CCGCCTGAGG TATTGCAGAT CGACACTGAC 
GAGACGTACA AAGCTCTCAC ATCGCACGAT GTCTGGCAAT TCGGCATTGT CCTATTTGTC 
TGTCTGACCG GATGCTTGCC GTGGCAGAAG GCGGCGTTGG ACGACCCGCG TTACACCAGA 
TATCAAAACT GGCACAACGC GACGCTGAAC ATAGCCAAGA AACCGAAATT ATTTCAGCTG 
ATCAGCTCAC GGGCGCAGAG GTTGTTCAAG CGTCTGCTGG ACCCGAAGGC CGAGAAGAGG 
CCTGTCAGCG TGCTGGAGGT AAACAAATAC CTGGAGGACA GGTGGCTGGC GAAACTGGGT 
GCGGAGAAGG CCATGAACGG TGGCGCCGAC GAGAGGGACG AGCTATGTCC CTCGATGTAC 
AGCTTCCACA GTTCGTTGGA GGAGAAGAAT CAGCTCCTTC ACACCCTGAC CACGTACGGA 
ATCGAGACTA CGGTTGACAG AGCGAAGAAG AAAGACCGTA TCAGAGAGTG GATTCAAGCG 
AGCGCGATAG TAGAAGAGTA CGAAGAGGAC GAGTCAGACC TGAACGAGGA CATCCGCTAC 
TACGAGGACG AGGACGAAGA GCCGAGTTCG ATGAAGGGTA GACACTTCCC GGAGACCCGT 
CCAATCGCCA AGAACGAGTC CAAACGACAC AGACACAGAA GCAACACCTC TAACCCAAGA 
AAGCGACAAA TCATCAAACC CACCGAGAGG AAGATCCCGT TCGCTCCTCA AGTGGCCGAA 
GAGCGACAGA CGATCGCCCG ATTCGCCAGT TTCCCGAACG GCGACCCGAA CCTGGCGGTG 
CTGAAGAATG GAACCAACAG CGACCCGGTC CTCCCGTTGA TGATCGAATC CAACACGGAG 
GAGACGAGGT CCGTTAACAA CGGCCAGGCC GACAGCGCCT CTCGAGAGGA CTCGTTACCG 
ACGAGTAGTT TCAAACCGAA CTTGCCCATC CAGGAGCCGG AGAACACGAA CGCTCAAGAT 
CAGAACTCGC AAACGGATCG CAGTTTGCCG AGAAGCCCGC AGATCCCGAC CAGGAAGCAT 
CGCTCGCAAA CTGCCCCGTT TCCGGCGCCG ACGTCGCCCG TGCTGAACGG AAACCGGGCC 
GAGTCCGCGA AGAGGAGTCA GTCGACCACC GTGCTGCCCA CGGACAGATC GAGCCATCGA 
TCGATGCCGA GCCAGTCGAA TGGCCAGCCC TCGGTGCCTC GGGGCGAAAG GTCGACCACG 
CAGCCGGAGA ATCGATCCGC GGCGCAATCA ATGTCTCGAG TAGAAAATCA ATCCGCGGTA 
ATGCTGCCGG CGACGGCTCG AGTGGAGAAT CAATCGAGTA ACGCTGTGCA GCTGGCCAGG 
AACGAGAAGT CCGCGAGCGG GCTAGTTCCC CTCGCGGCCG CAAATCCGCT GACGATGTCG 
GTCGCCTCTC ATCTGGTGAT GCAGAGCTTC AACGCGTTGC AGGGGATCAA CGCGGACGCT 
AACCACGCCG CCAGCCCGAA ATCGCCCAGA AACAGTCCCA ATTCTAACCA CAGCAAGATG 
TCCACGCCGA AGAACAGCCC CACGACTACG CCCGCGAGAA CCTCCACGCC ACCGAAACAC 
AAAATGAATC AGCGGACTGA AGAGACGGTC TTCTACGGAA AGGATCCTTT CGAGCACTAC 
GGTATCGCTC AGGGGGCGAT CCTGAGGGGC GATGCCCGAC GAGGCTCGCT CGAGAGCGCC 
AGCAGATCTG TCAGCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003699751.1
CDS	1205..3505
Translation	MSLMKKAIGGSIHRIREFELEKVNLIDEFDILQIVGEGWFGKILLTEHRSTQTEMVLKALPKAYTGISDFFREFHYGLHLAAHRNIITTYDVAFETAGFYVFSQEYAPLGDLTSNVTETGLGELHAKRVARQLAAAVHHIHSRELVHRDIKLDNILVFKSDFSRIKLCDFGETRRVNTVVRRHNEWLPYSPPEVLQIDTDETYKALTSHDVWQFGIVLFVCLTGCLPWQKAALDDPRYTRYQNWHNATLNIAKKPKLFQLISSRAQRLFKRLLDPKAEKRPVSVLEVNKYLEDRWLAKLGAEKAMNGGADERDELCPSMYSFHSSLEEKNQLLHTLTTYGIETTVDRAKKKDRIREWIQASAIVEEYEEDESDLNEDIRYYEDEDEEPSSMKGRHFPETRPIAKNESKRHRHRSNTSNPRKRQIIKPTERKIPFAPQVAEERQTIARFASFPNGDPNLAVLKNGTNSDPVLPLMIESNTEETRSVNNGQADSASREDSLPTSSFKPNLPIQEPENTNAQDQNSQTDRSLPRSPQIPTRKHRSQTAPFPAPTSPVLNGNRAESAKRSQSTTVLPTDRSSHRSMPSQSNGQPSVPRGERSTTQPENRSAAQSMSRVENQSAVMLPATARVENQSSNAVQLARNEKSASGLVPLAAANPLTMSVASHLVMQSFNALQGINADANHAASPKSPRNSPNSNHSKMSTPKNSPTTTPARTSTPPKHKMNQRTEETVFYGKDPFEHYGIAQGAILRGDARRGSLESASRSVSN

Target ORF information:

RefSeq Version	XM_003699703.2
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata serine/threonine-protein kinase Nek3 (LOC100883271), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003699703.2

ORF Insert Sequence:

ATGAGCTTAA TGAAGAAGGC GATCGGCGGT TCCATTCACA GGATACGCGA ATTCGAGCTC 
GAGAAGGTGA ATCTGATCGA CGAGTTCGAT ATCCTGCAAA TCGTTGGCGA GGGATGGTTC 
GGCAAGATCC TGCTTACGGA GCATCGCAGC ACCCAGACAG AGATGGTGCT GAAAGCGCTG 
CCGAAGGCTT ACACCGGCAT CTCGGACTTC TTTCGGGAAT TCCATTATGG GCTACACCTG 
GCTGCGCACA GGAACATCAT CACCACCTAC GATGTGGCCT TCGAGACCGC TGGCTTCTAC 
GTTTTCAGTC AGGAGTACGC TCCTCTCGGA GATCTAACGT CGAACGTGAC AGAGACCGGT 
TTAGGCGAGC TCCACGCGAA GAGGGTTGCC AGACAACTGG CTGCAGCGGT GCATCACATT 
CACAGCCGCG AATTGGTGCA TCGTGACATC AAACTTGACA ACATCCTCGT GTTCAAGAGT 
GACTTCTCGC GAATCAAATT GTGCGACTTC GGCGAGACCA GACGAGTCAA CACTGTAGTC 
CGAAGGCACA ACGAGTGGCT GCCATACTCG CCGCCTGAGG TATTGCAGAT CGACACTGAC 
GAGACGTACA AAGCTCTCAC ATCGCACGAT GTCTGGCAAT TCGGCATTGT CCTATTTGTC 
TGTCTGACCG GATGCTTGCC GTGGCAGAAG GCGGCGTTGG ACGACCCGCG TTACACCAGA 
TATCAAAACT GGCACAACGC GACGCTGAAC ATAGCCAAGA AACCGAAATT ATTTCAGCTG 
ATCAGCTCAC GGGCGCAGAG GTTGTTCAAG CGTCTGCTGG ACCCGAAGGC CGAGAAGAGG 
CCTGTCAGCG TGCTGGAGGT AAACAAATAC CTGGAGGACA GGTGGCTGGC GAAACTGGGT 
GCGGAGAAGG CCATGAACGG TGGCGCCGAC GAGAGGGACG AGCTATGTCC CTCGATGTAC 
AGCTTCCACA GTTCGTTGGA GGAGAAGAAT CAGCTCCTTC ACACCCTGAC CACGTACGGA 
ATCGAGACTA CGGTTGACAG AGCGAAGAAG AAAGACCGTA TCAGAGAGTG GATTCAAGCG 
AGCGCGATAG TAGAAGAGTA CGAAGAGGAC GAGTCAGACC TGAACGAGGA CATCCGCTAC 
TACGAGGACG AGGACGAAGA GCCGAGTTCG ATGAAGGGTA GACACTTCCC GGAGACCCGT 
CCAATCGCCA AGAACGAGTC CAAACGACAC AGACACAGAA GCAACACCTC TAACCCAAGA 
AAGCGACAAA TCATCAAACC CACCGAGAGG AAGATCCCGT TCGCTCCTCA AGTGGCCGAA 
GAGCGACAGA CGATCGCCCG ATTCGCCAGT TTCCCGAACG GCGACCCGAA CCTGGCGGTG 
CTGAAGAATG GAACCAACAG CGACCCGGTC CTCCCGTTGA TGATCGAATC CAACACGGAG 
GAGACGAGGT CCGTTAACAA CGGCCAGGCC GACAGCGCCT CTCGAGAGGA CTCGTTACCG 
ACGAGTAGTT TCAAACCGAA CTTGCCCATC CAGGAGCCGG AGAACACGAA CGCTCAAGAT 
CAGAACTCGC AAACGGATCG CAGTTTGCCG AGAAGCCCGC AGATCCCGAC CAGGAAGCAT 
CGCTCGCAAA CTGCCCCGTT TCCGGCGCCG ACGTCGCCCG TGCTGAACGG AAACCGGGCC 
GAGTCCGCGA AGAGGAGTCA GTCGACCACC GTGCTGCCCA CGGACAGATC GAGCCATCGA 
TCGATGCCGA GCCAGTCGAA TGGCCAGCCC TCGGTGCCTC GGGGCGAAAG GTCGACCACG 
CAGCCGGAGA ATCGATCCGC GGCGCAATCA ATGTCTCGAG TAGAAAATCA ATCCGCGGTA 
ATGCTGCCGG CGACGGCTCG AGTGGAGAAT CAATCGAGTA ACGCTGTGCA GCTGGCCAGG 
AACGAGAAGT CCGCGAGCGG GCTAGTTCCC CTCGCGGCCG CAAATCCGCT GACGATGTCG 
GTCGCCTCTC ATCTGGTGAT GCAGAGCTTC AACGCGTTGC AGGGGATCAA CGCGGACGCT 
AACCACGCCG CCAGCCCGAA ATCGCCCAGA AACAGTCCCA ATTCTAACCA CAGCAAGATG 
TCCACGCCGA AGAACAGCCC CACGACTACG CCCGCGAGAA CCTCCACGCC ACCGAAACAC 
AAAATGAATC AGCGGACTGA AGAGACGGTC TTCTACGGAA AGGATCCTTT CGAGCACTAC 
GGTATCGCTC AGGGGGCGAT CCTGAGGGGC GATGCCCGAC GAGGCTCGCT CGAGAGCGCC 
AGCAGATCTG TCAGCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100883271 ( XM_012288280.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012143670.1 Megachile rotundata serine/threonine-protein kinase Nek3 (LOC100883271), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMe97430
Clone ID Related Accession (Same CDS sequence)	XM_003699703.2 , XM_012288280.1
Accession Version	XM_012288280.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	serine/threonine-protein kinase Nek3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797077.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTAA TGAAGAAGGC GATCGGCGGT TCCATTCACA GGATACGCGA ATTCGAGCTC 
GAGAAGGTGA ATCTGATCGA CGAGTTCGAT ATCCTGCAAA TCGTTGGCGA GGGATGGTTC 
GGCAAGATCC TGCTTACGGA GCATCGCAGC ACCCAGACAG AGATGGTGCT GAAAGCGCTG 
CCGAAGGCTT ACACCGGCAT CTCGGACTTC TTTCGGGAAT TCCATTATGG GCTACACCTG 
GCTGCGCACA GGAACATCAT CACCACCTAC GATGTGGCCT TCGAGACCGC TGGCTTCTAC 
GTTTTCAGTC AGGAGTACGC TCCTCTCGGA GATCTAACGT CGAACGTGAC AGAGACCGGT 
TTAGGCGAGC TCCACGCGAA GAGGGTTGCC AGACAACTGG CTGCAGCGGT GCATCACATT 
CACAGCCGCG AATTGGTGCA TCGTGACATC AAACTTGACA ACATCCTCGT GTTCAAGAGT 
GACTTCTCGC GAATCAAATT GTGCGACTTC GGCGAGACCA GACGAGTCAA CACTGTAGTC 
CGAAGGCACA ACGAGTGGCT GCCATACTCG CCGCCTGAGG TATTGCAGAT CGACACTGAC 
GAGACGTACA AAGCTCTCAC ATCGCACGAT GTCTGGCAAT TCGGCATTGT CCTATTTGTC 
TGTCTGACCG GATGCTTGCC GTGGCAGAAG GCGGCGTTGG ACGACCCGCG TTACACCAGA 
TATCAAAACT GGCACAACGC GACGCTGAAC ATAGCCAAGA AACCGAAATT ATTTCAGCTG 
ATCAGCTCAC GGGCGCAGAG GTTGTTCAAG CGTCTGCTGG ACCCGAAGGC CGAGAAGAGG 
CCTGTCAGCG TGCTGGAGGT AAACAAATAC CTGGAGGACA GGTGGCTGGC GAAACTGGGT 
GCGGAGAAGG CCATGAACGG TGGCGCCGAC GAGAGGGACG AGCTATGTCC CTCGATGTAC 
AGCTTCCACA GTTCGTTGGA GGAGAAGAAT CAGCTCCTTC ACACCCTGAC CACGTACGGA 
ATCGAGACTA CGGTTGACAG AGCGAAGAAG AAAGACCGTA TCAGAGAGTG GATTCAAGCG 
AGCGCGATAG TAGAAGAGTA CGAAGAGGAC GAGTCAGACC TGAACGAGGA CATCCGCTAC 
TACGAGGACG AGGACGAAGA GCCGAGTTCG ATGAAGGGTA GACACTTCCC GGAGACCCGT 
CCAATCGCCA AGAACGAGTC CAAACGACAC AGACACAGAA GCAACACCTC TAACCCAAGA 
AAGCGACAAA TCATCAAACC CACCGAGAGG AAGATCCCGT TCGCTCCTCA AGTGGCCGAA 
GAGCGACAGA CGATCGCCCG ATTCGCCAGT TTCCCGAACG GCGACCCGAA CCTGGCGGTG 
CTGAAGAATG GAACCAACAG CGACCCGGTC CTCCCGTTGA TGATCGAATC CAACACGGAG 
GAGACGAGGT CCGTTAACAA CGGCCAGGCC GACAGCGCCT CTCGAGAGGA CTCGTTACCG 
ACGAGTAGTT TCAAACCGAA CTTGCCCATC CAGGAGCCGG AGAACACGAA CGCTCAAGAT 
CAGAACTCGC AAACGGATCG CAGTTTGCCG AGAAGCCCGC AGATCCCGAC CAGGAAGCAT 
CGCTCGCAAA CTGCCCCGTT TCCGGCGCCG ACGTCGCCCG TGCTGAACGG AAACCGGGCC 
GAGTCCGCGA AGAGGAGTCA GTCGACCACC GTGCTGCCCA CGGACAGATC GAGCCATCGA 
TCGATGCCGA GCCAGTCGAA TGGCCAGCCC TCGGTGCCTC GGGGCGAAAG GTCGACCACG 
CAGCCGGAGA ATCGATCCGC GGCGCAATCA ATGTCTCGAG TAGAAAATCA ATCCGCGGTA 
ATGCTGCCGG CGACGGCTCG AGTGGAGAAT CAATCGAGTA ACGCTGTGCA GCTGGCCAGG 
AACGAGAAGT CCGCGAGCGG GCTAGTTCCC CTCGCGGCCG CAAATCCGCT GACGATGTCG 
GTCGCCTCTC ATCTGGTGAT GCAGAGCTTC AACGCGTTGC AGGGGATCAA CGCGGACGCT 
AACCACGCCG CCAGCCCGAA ATCGCCCAGA AACAGTCCCA ATTCTAACCA CAGCAAGATG 
TCCACGCCGA AGAACAGCCC CACGACTACG CCCGCGAGAA CCTCCACGCC ACCGAAACAC 
AAAATGAATC AGCGGACTGA AGAGACGGTC TTCTACGGAA AGGATCCTTT CGAGCACTAC 
GGTATCGCTC AGGGGGCGAT CCTGAGGGGC GATGCCCGAC GAGGCTCGCT CGAGAGCGCC 
AGCAGATCTG TCAGCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012143670.1
CDS	208..2508
Translation	MSLMKKAIGGSIHRIREFELEKVNLIDEFDILQIVGEGWFGKILLTEHRSTQTEMVLKALPKAYTGISDFFREFHYGLHLAAHRNIITTYDVAFETAGFYVFSQEYAPLGDLTSNVTETGLGELHAKRVARQLAAAVHHIHSRELVHRDIKLDNILVFKSDFSRIKLCDFGETRRVNTVVRRHNEWLPYSPPEVLQIDTDETYKALTSHDVWQFGIVLFVCLTGCLPWQKAALDDPRYTRYQNWHNATLNIAKKPKLFQLISSRAQRLFKRLLDPKAEKRPVSVLEVNKYLEDRWLAKLGAEKAMNGGADERDELCPSMYSFHSSLEEKNQLLHTLTTYGIETTVDRAKKKDRIREWIQASAIVEEYEEDESDLNEDIRYYEDEDEEPSSMKGRHFPETRPIAKNESKRHRHRSNTSNPRKRQIIKPTERKIPFAPQVAEERQTIARFASFPNGDPNLAVLKNGTNSDPVLPLMIESNTEETRSVNNGQADSASREDSLPTSSFKPNLPIQEPENTNAQDQNSQTDRSLPRSPQIPTRKHRSQTAPFPAPTSPVLNGNRAESAKRSQSTTVLPTDRSSHRSMPSQSNGQPSVPRGERSTTQPENRSAAQSMSRVENQSAVMLPATARVENQSSNAVQLARNEKSASGLVPLAAANPLTMSVASHLVMQSFNALQGINADANHAASPKSPRNSPNSNHSKMSTPKNSPTTTPARTSTPPKHKMNQRTEETVFYGKDPFEHYGIAQGAILRGDARRGSLESASRSVSN

Target ORF information:

RefSeq Version	XM_012288280.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata serine/threonine-protein kinase Nek3 (LOC100883271), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012288280.1

ORF Insert Sequence:

ATGAGCTTAA TGAAGAAGGC GATCGGCGGT TCCATTCACA GGATACGCGA ATTCGAGCTC 
GAGAAGGTGA ATCTGATCGA CGAGTTCGAT ATCCTGCAAA TCGTTGGCGA GGGATGGTTC 
GGCAAGATCC TGCTTACGGA GCATCGCAGC ACCCAGACAG AGATGGTGCT GAAAGCGCTG 
CCGAAGGCTT ACACCGGCAT CTCGGACTTC TTTCGGGAAT TCCATTATGG GCTACACCTG 
GCTGCGCACA GGAACATCAT CACCACCTAC GATGTGGCCT TCGAGACCGC TGGCTTCTAC 
GTTTTCAGTC AGGAGTACGC TCCTCTCGGA GATCTAACGT CGAACGTGAC AGAGACCGGT 
TTAGGCGAGC TCCACGCGAA GAGGGTTGCC AGACAACTGG CTGCAGCGGT GCATCACATT 
CACAGCCGCG AATTGGTGCA TCGTGACATC AAACTTGACA ACATCCTCGT GTTCAAGAGT 
GACTTCTCGC GAATCAAATT GTGCGACTTC GGCGAGACCA GACGAGTCAA CACTGTAGTC 
CGAAGGCACA ACGAGTGGCT GCCATACTCG CCGCCTGAGG TATTGCAGAT CGACACTGAC 
GAGACGTACA AAGCTCTCAC ATCGCACGAT GTCTGGCAAT TCGGCATTGT CCTATTTGTC 
TGTCTGACCG GATGCTTGCC GTGGCAGAAG GCGGCGTTGG ACGACCCGCG TTACACCAGA 
TATCAAAACT GGCACAACGC GACGCTGAAC ATAGCCAAGA AACCGAAATT ATTTCAGCTG 
ATCAGCTCAC GGGCGCAGAG GTTGTTCAAG CGTCTGCTGG ACCCGAAGGC CGAGAAGAGG 
CCTGTCAGCG TGCTGGAGGT AAACAAATAC CTGGAGGACA GGTGGCTGGC GAAACTGGGT 
GCGGAGAAGG CCATGAACGG TGGCGCCGAC GAGAGGGACG AGCTATGTCC CTCGATGTAC 
AGCTTCCACA GTTCGTTGGA GGAGAAGAAT CAGCTCCTTC ACACCCTGAC CACGTACGGA 
ATCGAGACTA CGGTTGACAG AGCGAAGAAG AAAGACCGTA TCAGAGAGTG GATTCAAGCG 
AGCGCGATAG TAGAAGAGTA CGAAGAGGAC GAGTCAGACC TGAACGAGGA CATCCGCTAC 
TACGAGGACG AGGACGAAGA GCCGAGTTCG ATGAAGGGTA GACACTTCCC GGAGACCCGT 
CCAATCGCCA AGAACGAGTC CAAACGACAC AGACACAGAA GCAACACCTC TAACCCAAGA 
AAGCGACAAA TCATCAAACC CACCGAGAGG AAGATCCCGT TCGCTCCTCA AGTGGCCGAA 
GAGCGACAGA CGATCGCCCG ATTCGCCAGT TTCCCGAACG GCGACCCGAA CCTGGCGGTG 
CTGAAGAATG GAACCAACAG CGACCCGGTC CTCCCGTTGA TGATCGAATC CAACACGGAG 
GAGACGAGGT CCGTTAACAA CGGCCAGGCC GACAGCGCCT CTCGAGAGGA CTCGTTACCG 
ACGAGTAGTT TCAAACCGAA CTTGCCCATC CAGGAGCCGG AGAACACGAA CGCTCAAGAT 
CAGAACTCGC AAACGGATCG CAGTTTGCCG AGAAGCCCGC AGATCCCGAC CAGGAAGCAT 
CGCTCGCAAA CTGCCCCGTT TCCGGCGCCG ACGTCGCCCG TGCTGAACGG AAACCGGGCC 
GAGTCCGCGA AGAGGAGTCA GTCGACCACC GTGCTGCCCA CGGACAGATC GAGCCATCGA 
TCGATGCCGA GCCAGTCGAA TGGCCAGCCC TCGGTGCCTC GGGGCGAAAG GTCGACCACG 
CAGCCGGAGA ATCGATCCGC GGCGCAATCA ATGTCTCGAG TAGAAAATCA ATCCGCGGTA 
ATGCTGCCGG CGACGGCTCG AGTGGAGAAT CAATCGAGTA ACGCTGTGCA GCTGGCCAGG 
AACGAGAAGT CCGCGAGCGG GCTAGTTCCC CTCGCGGCCG CAAATCCGCT GACGATGTCG 
GTCGCCTCTC ATCTGGTGAT GCAGAGCTTC AACGCGTTGC AGGGGATCAA CGCGGACGCT 
AACCACGCCG CCAGCCCGAA ATCGCCCAGA AACAGTCCCA ATTCTAACCA CAGCAAGATG 
TCCACGCCGA AGAACAGCCC CACGACTACG CCCGCGAGAA CCTCCACGCC ACCGAAACAC 
AAAATGAATC AGCGGACTGA AGAGACGGTC TTCTACGGAA AGGATCCTTT CGAGCACTAC 
GGTATCGCTC AGGGGGCGAT CCTGAGGGGC GATGCCCGAC GAGGCTCGCT CGAGAGCGCC 
AGCAGATCTG TCAGCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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LOC100883271 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム