LOC100883005 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100883005
Entrez Gene ID	100883005
Full Name	replication factor C subunit 4
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003705709.2	XP_003705757.1	replication factor C subunit 4

Pathways from BioSystems
Gene Ontology

The following LOC100883005 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100883005 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe109596	XM_003705709.2 Latest version!	Megachile rotundata replication factor C subunit 4 (LOC100883005), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100883005 ( XM_003705709.2 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_003705757.1 Megachile rotundata replication factor C subunit 4 (LOC100883005), mRNA.

Price & Availability↑

CloneID	OMe109596
Clone ID Related Accession (Same CDS sequence)	XM_003705709.2
Accession Version	XM_003705709.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	replication factor C subunit 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797325.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2015 this sequence version replaced XM_003705709.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGCTT TCTTAAAAAC AGGGAAATTA GGTCCTGAAG GGTCTAAAAA ACCTTCGACT 
TCACGCACAA AGGAAAAACG CAGTGCTCCT CCACCGCCAT GGGTAGAAAA ATATCGTCCA 
AAAACTGTAG AAGATGTCGT TGAACAAGCA GAAGTTGTGG AGGTGTTACG ACAATGTTTA 
AGCGGTAGTG ACTTTCCAAA TTTATTATTT TATGGACCAC CCGGCACTGG CAAAACAAGT 
ACAATACTAG CAGCGGCTAG ACAATTGTTT GGTAGCTTGT ACAAAGACAG GATATTAGAA 
TTAAATGCTT CTGATGAAAG GGGTATCCAA GTGGTAAGGG ATAAGATTAA AACCTTTGCA 
CAACTTACAG CAGGTGGCAT GAGGGACGAT GGAAAACGAT GTCCACCTTT CAAAATTATT 
ATTTTGGACG AAGCAGATAG CATGACCGCC GCGGCACAAG CAGCTCTCCG TCGCACCATG 
GAGAAGGAAT CTCACAGTAC TCGTTTTTGT TTAATTTGTA ACTATGTATC AAGAATTATA 
GAACCGCTCA CTTCGCGTTG TACGAAATTC AGATTCAAAC CACTGGGAGA AGAAAAAATT 
ATTGAAAGAT TGGAATATAT ATCTAACGAA GAAGGTTTAA AAGCCGGCAA GCCTGTTTTG 
TTAAAAATCG TCGAAGCCTC GGGTGGAGAC ATGAGACGAG CCATCACGTG CCTGCAGTCT 
ATAACTAGAT TAAAGGGCAA AGACATTGAA ATTACCATTG ATGACATTGT GGAAATTATT 
GGGATTGTTC CTGATAAGTG GATTGATGAA TTAATGGAAG TATGTAAAAC AAAGGATTAT 
AGTAAGGCAG AAGCATTTAT TGACACGTTT ATGTTAGAAG CATATGCCGC GTCACAAGTA 
ATCGAACAGC TTAGCGAGAG GATTATATAT TCTAACGAGT TTACAGACAG ACAAAAAGCT 
TTAATCGCGG ATAGACTCGG AGAGTGTAAC TACAGACTGC TAGATGGAGG GAGCGAGTAC 
ATACAACTTA TAAATCTTTG TTGCGGCATT ATAAAGGCTT ACGAATTAGA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003705757.1
CDS	70..1143
Translation	MQAFLKTGKLGPEGSKKPSTSRTKEKRSAPPPPWVEKYRPKTVEDVVEQAEVVEVLRQCLSGSDFPNLLFYGPPGTGKTSTILAAARQLFGSLYKDRILELNASDERGIQVVRDKIKTFAQLTAGGMRDDGKRCPPFKIIILDEADSMTAAAQAALRRTMEKESHSTRFCLICNYVSRIIEPLTSRCTKFRFKPLGEEKIIERLEYISNEEGLKAGKPVLLKIVEASGGDMRRAITCLQSITRLKGKDIEITIDDIVEIIGIVPDKWIDELMEVCKTKDYSKAEAFIDTFMLEAYAASQVIEQLSERIIYSNEFTDRQKALIADRLGECNYRLLDGGSEYIQLINLCCGIIKAYELE

Target ORF information:

RefSeq Version	XM_003705709.2
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata replication factor C subunit 4 (LOC100883005), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003705709.2

ORF Insert Sequence:

ATGCAAGCTT TCTTAAAAAC AGGGAAATTA GGTCCTGAAG GGTCTAAAAA ACCTTCGACT 
TCACGCACAA AGGAAAAACG CAGTGCTCCT CCACCGCCAT GGGTAGAAAA ATATCGTCCA 
AAAACTGTAG AAGATGTCGT TGAACAAGCA GAAGTTGTGG AGGTGTTACG ACAATGTTTA 
AGCGGTAGTG ACTTTCCAAA TTTATTATTT TATGGACCAC CCGGCACTGG CAAAACAAGT 
ACAATACTAG CAGCGGCTAG ACAATTGTTT GGTAGCTTGT ACAAAGACAG GATATTAGAA 
TTAAATGCTT CTGATGAAAG GGGTATCCAA GTGGTAAGGG ATAAGATTAA AACCTTTGCA 
CAACTTACAG CAGGTGGCAT GAGGGACGAT GGAAAACGAT GTCCACCTTT CAAAATTATT 
ATTTTGGACG AAGCAGATAG CATGACCGCC GCGGCACAAG CAGCTCTCCG TCGCACCATG 
GAGAAGGAAT CTCACAGTAC TCGTTTTTGT TTAATTTGTA ACTATGTATC AAGAATTATA 
GAACCGCTCA CTTCGCGTTG TACGAAATTC AGATTCAAAC CACTGGGAGA AGAAAAAATT 
ATTGAAAGAT TGGAATATAT ATCTAACGAA GAAGGTTTAA AAGCCGGCAA GCCTGTTTTG 
TTAAAAATCG TCGAAGCCTC GGGTGGAGAC ATGAGACGAG CCATCACGTG CCTGCAGTCT 
ATAACTAGAT TAAAGGGCAA AGACATTGAA ATTACCATTG ATGACATTGT GGAAATTATT 
GGGATTGTTC CTGATAAGTG GATTGATGAA TTAATGGAAG TATGTAAAAC AAAGGATTAT 
AGTAAGGCAG AAGCATTTAT TGACACGTTT ATGTTAGAAG CATATGCCGC GTCACAAGTA 
ATCGAACAGC TTAGCGAGAG GATTATATAT TCTAACGAGT TTACAGACAG ACAAAAAGCT 
TTAATCGCGG ATAGACTCGG AGAGTGTAAC TACAGACTGC TAGATGGAGG GAGCGAGTAC 
ATACAACTTA TAAATCTTTG TTGCGGCATT ATAAAGGCTT ACGAATTAGA ATAG