LOC100882207 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100882207
Entrez Gene ID	100882207
Full Name	N-alpha-acetyltransferase 25, NatB auxiliary subunit
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012280699.1	XP_012136089.1	N-alpha-acetyltransferase 25, NatB auxiliary subunit isoform X2
XM_012280691.1	XP_012136081.1	N-alpha-acetyltransferase 25, NatB auxiliary subunit isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC100882207 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100882207 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe96751	XM_012280691.1 Latest version!	Megachile rotundata N-alpha-acetyltransferase 25, NatB auxiliary subunit (LOC100882207), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMe96752	XM_012280699.1 Latest version!	Megachile rotundata N-alpha-acetyltransferase 25, NatB auxiliary subunit (LOC100882207), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100882207 ( XM_012280691.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012136081.1 Megachile rotundata N-alpha-acetyltransferase 25, NatB auxiliary subunit (LOC100882207), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMe96751
Clone ID Related Accession (Same CDS sequence)	XM_012280691.1
Accession Version	XM_012280691.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2715bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	N-alpha-acetyltransferase 25, NatB auxiliary subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797060.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCAA AGACCCATGT GGACCATACC GTTAACGAAC GTCGTTTACG TCCAATATAC 
AATTGGTTGG ATAATGGAAA TAATAAAAAA GCTCTTCAAG AAGCAGATAA AGTGCTTAAA 
AAGCAACCAA CTAATCAGTG TGCCAGGGTG CTCAAAGCTT TGGCCTTATT ACGACTTGGC 
AAAGAAAATG AATGTCAAAC TATTATGGAT AAAGTACGCT CAGAAATTCC TTGCGAAGAT 
TCTACACTGC AAGTTATGAG TATTTGTTAC AGAGAAAGAC GTCAGCCTGA TAAAATTAGT 
GAAGTATACG AAGCTGCAGC AAAAGCAGAT CCTAACAATG AAGAGTTATT AACACATCTT 
TTTATGTCTT ATGTTCGTCT TGGAGACTAT AAGAAACAAC AACAAACGGC ACTTGCCTTA 
TATAAATTGA AACCAAAAAA TCCTTATTAT TTTTGGGCTG TGATGAGCAT AGTTATGCAA 
GCTATACATG CAGATGAAAA CTTAGCTAAA GGAATTACTT TACCTTTAGC AGAAAGAATG 
GTACTAAAAT TAGTTAAAGA AGGAAAAATG GAAGCAGAAC AAGAAGTACA ACTTTATTTA 
ATGATATTAG AACTTCAAGA TAAAAGTGAA GAAATGTTAA ATGTTTTATC TGGACCCTTA 
GCATCACATC TTTCATCTGT GTCCCAACGT AAGGCAGCAC TTTTATTAAA ATTAGAACGT 
TTTCCTGAAG CTGCTAGTGC ATATAAAGAA CTTATTAGTG AAAATACAGA CAATTGGGCA 
TATTATAAAG ACTATCTCTT TGCTGCACTT AAATTTCAAA AACCAGAAGA ATGTTTAAAT 
TTTCTTAACA AAATTATAAC TATGTCGGAG AAAACAGTAA GAGCTCCTTA CCTTGCAAAA 
TTCGAATTAT TAAAACGTAC TAAAAACGGA GAAGTTATTG AAAATTGTAT TGAACCAGTA 
GATTTAATGT ATCAATATTT CTCGAAATTT GGGGAAAAAG ACTGTATAGT TGGAGATTTG 
CGACTTTATT TAGATCTGTT AACTCCTACT GGAAAACAAC AGTTACTTCA AAAAATAGAG 
GAAGATGTAG GTGTTAAGGC AGAAGGTTTT CCAAGCACTG TACAACAAAT GCAGAAACAC 
ATTCACTTAG AACAATTGCG ACGCATTTGT GGTTTCCATC ATTCTCCAAA TGCTGATATA 
AACAAGCAAA AACAATTGAT AGAACGATTA TGTAAATTAT ACGAAAAGGG CAATGAATTG 
TGTCCAATTC AAGAAAGATT ACCAACAGAT TTTTGCCCTG CAGATTCTTA CATTCTTTTA 
GCTTCACATT TATTACATGA ATTATTTTAT AATACAAATG ACGCAGTTTA TCTTTACAGG 
GCAATGGCAT TATTAGAACG TGGTTTATTA TCGAGCCCTG CAAATTTCCA TATAAAAATT 
TTACTAGTAC GAATATATTT AGAAGCTGGA TTAGTTGGGG CAGCCGATCA TATTTTCACG 
TTATTGGACG TTAAACATAT TCAATTAGAT TCGTTGGGTT ATTTACATAC ACCATTACTT 
GCTCCACTTG GCCACCTTTC GTTAGCTTCC ATAACTTTAG ATCATGCTAC TAAATTTTTC 
ATTGCAAATT ACAAAGATAG CGCGGATCAT TTAACGTTCG CCTATAAATA TGGAAGTTTT 
GTAAAGATTC AAGAATTTGT GGAGTTAAAA GAACGATTGG AAAATTGTTT TCAATTTGTC 
ATGACTACTT TAGATAAAAT GCTTTTGGAA TTAAGTTGGT GTAATAGCCC TACAACTTTG 
ATTTCTACGT TAAGTAATAT GGATATTCAA CTTAAGGAGG ATTCGATTCG TTTGAATTCG 
CTGCGGGACA ACCGTGATTT AGAGGTTGTT CGTGGGTGGG AACCACTCAC AGAAAATGGC 
GAAGATCTCA GAATGCAAGA AGAAACACGA ATTTGTATGT TAAGATTATT TATGGCGAGA 
AATTTAATAT TGAAAATTAT AGCAGCGTGT GTGGCACCCG ATAGTTCCTC TCTTCTTACC 
CGTTTTTCCG ACGAACTAAA GCAGTTGGAT AGCGAAGAAA TTCCTTCGAT TCTTCAAGAG 
TTTGAACCAG AAGGAAAGCA AAATAGGCCA TGTAAAATAC TAGTTCCTAT GGATGCAGTC 
GAAAGGTTAC GTGAAGCCCA TTATTCTGAA CAGTTGAAAA CAATTGCTCG ACTTGTTGAA 
TGTTTTGCTA ATTCGCGTTA CCCCGATTTT GAGTGCATTC AAATGTTACG TGCATCTCCA 
TGCCTTCAGA CAATAGATAT CCCGGAGAAG GGAATTCCAG TGTCTTTCAA GCATTTTTTA 
CTGAGAGCAT CCACGTGTAG TGAATCTCTT GCAATTATTA GTGCCATATG TACTTTATGG 
GCGATGCAAT TACAACCTCA GACTTCAGAG AAGAAAAATA AAAAGAAGTG TAACAAGCAA 
ATAGCACATA TTCCATTAAA TGATAATGAT CATAATTGGA AGGAAATTGC AATATTACTT 
TCAGAGAAAG TAGATAATTT AGATTCAGTA CTAACAGAAT TTGAAAAGTA TCAACTTAAC 
ACTGGACTAG ATAATGAAGA TGATGTTTGT GCTGCGATAC TCAAACATGG TCAAGCCAGT 
TTAGGACAAA GTTGCAGATC GTTAAAGTCT CGAGCTCAAA TCACGTTAAA GCTATTAAGC 
AGTTTAAAAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012136081.1
CDS	262..2976
Translation	MASKTHVDHTVNERRLRPIYNWLDNGNNKKALQEADKVLKKQPTNQCARVLKALALLRLGKENECQTIMDKVRSEIPCEDSTLQVMSICYRERRQPDKISEVYEAAAKADPNNEELLTHLFMSYVRLGDYKKQQQTALALYKLKPKNPYYFWAVMSIVMQAIHADENLAKGITLPLAERMVLKLVKEGKMEAEQEVQLYLMILELQDKSEEMLNVLSGPLASHLSSVSQRKAALLLKLERFPEAASAYKELISENTDNWAYYKDYLFAALKFQKPEECLNFLNKIITMSEKTVRAPYLAKFELLKRTKNGEVIENCIEPVDLMYQYFSKFGEKDCIVGDLRLYLDLLTPTGKQQLLQKIEEDVGVKAEGFPSTVQQMQKHIHLEQLRRICGFHHSPNADINKQKQLIERLCKLYEKGNELCPIQERLPTDFCPADSYILLASHLLHELFYNTNDAVYLYRAMALLERGLLSSPANFHIKILLVRIYLEAGLVGAADHIFTLLDVKHIQLDSLGYLHTPLLAPLGHLSLASITLDHATKFFIANYKDSADHLTFAYKYGSFVKIQEFVELKERLENCFQFVMTTLDKMLLELSWCNSPTTLISTLSNMDIQLKEDSIRLNSLRDNRDLEVVRGWEPLTENGEDLRMQEETRICMLRLFMARNLILKIIAACVAPDSSSLLTRFSDELKQLDSEEIPSILQEFEPEGKQNRPCKILVPMDAVERLREAHYSEQLKTIARLVECFANSRYPDFECIQMLRASPCLQTIDIPEKGIPVSFKHFLLRASTCSESLAIISAICTLWAMQLQPQTSEKKNKKKCNKQIAHIPLNDNDHNWKEIAILLSEKVDNLDSVLTEFEKYQLNTGLDNEDDVCAAILKHGQASLGQSCRSLKSRAQITLKLLSSLKS

Target ORF information:

RefSeq Version	XM_012280691.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata N-alpha-acetyltransferase 25, NatB auxiliary subunit (LOC100882207), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012280691.1

ORF Insert Sequence:

ATGGCGTCAA AGACCCATGT GGACCATACC GTTAACGAAC GTCGTTTACG TCCAATATAC 
AATTGGTTGG ATAATGGAAA TAATAAAAAA GCTCTTCAAG AAGCAGATAA AGTGCTTAAA 
AAGCAACCAA CTAATCAGTG TGCCAGGGTG CTCAAAGCTT TGGCCTTATT ACGACTTGGC 
AAAGAAAATG AATGTCAAAC TATTATGGAT AAAGTACGCT CAGAAATTCC TTGCGAAGAT 
TCTACACTGC AAGTTATGAG TATTTGTTAC AGAGAAAGAC GTCAGCCTGA TAAAATTAGT 
GAAGTATACG AAGCTGCAGC AAAAGCAGAT CCTAACAATG AAGAGTTATT AACACATCTT 
TTTATGTCTT ATGTTCGTCT TGGAGACTAT AAGAAACAAC AACAAACGGC ACTTGCCTTA 
TATAAATTGA AACCAAAAAA TCCTTATTAT TTTTGGGCTG TGATGAGCAT AGTTATGCAA 
GCTATACATG CAGATGAAAA CTTAGCTAAA GGAATTACTT TACCTTTAGC AGAAAGAATG 
GTACTAAAAT TAGTTAAAGA AGGAAAAATG GAAGCAGAAC AAGAAGTACA ACTTTATTTA 
ATGATATTAG AACTTCAAGA TAAAAGTGAA GAAATGTTAA ATGTTTTATC TGGACCCTTA 
GCATCACATC TTTCATCTGT GTCCCAACGT AAGGCAGCAC TTTTATTAAA ATTAGAACGT 
TTTCCTGAAG CTGCTAGTGC ATATAAAGAA CTTATTAGTG AAAATACAGA CAATTGGGCA 
TATTATAAAG ACTATCTCTT TGCTGCACTT AAATTTCAAA AACCAGAAGA ATGTTTAAAT 
TTTCTTAACA AAATTATAAC TATGTCGGAG AAAACAGTAA GAGCTCCTTA CCTTGCAAAA 
TTCGAATTAT TAAAACGTAC TAAAAACGGA GAAGTTATTG AAAATTGTAT TGAACCAGTA 
GATTTAATGT ATCAATATTT CTCGAAATTT GGGGAAAAAG ACTGTATAGT TGGAGATTTG 
CGACTTTATT TAGATCTGTT AACTCCTACT GGAAAACAAC AGTTACTTCA AAAAATAGAG 
GAAGATGTAG GTGTTAAGGC AGAAGGTTTT CCAAGCACTG TACAACAAAT GCAGAAACAC 
ATTCACTTAG AACAATTGCG ACGCATTTGT GGTTTCCATC ATTCTCCAAA TGCTGATATA 
AACAAGCAAA AACAATTGAT AGAACGATTA TGTAAATTAT ACGAAAAGGG CAATGAATTG 
TGTCCAATTC AAGAAAGATT ACCAACAGAT TTTTGCCCTG CAGATTCTTA CATTCTTTTA 
GCTTCACATT TATTACATGA ATTATTTTAT AATACAAATG ACGCAGTTTA TCTTTACAGG 
GCAATGGCAT TATTAGAACG TGGTTTATTA TCGAGCCCTG CAAATTTCCA TATAAAAATT 
TTACTAGTAC GAATATATTT AGAAGCTGGA TTAGTTGGGG CAGCCGATCA TATTTTCACG 
TTATTGGACG TTAAACATAT TCAATTAGAT TCGTTGGGTT ATTTACATAC ACCATTACTT 
GCTCCACTTG GCCACCTTTC GTTAGCTTCC ATAACTTTAG ATCATGCTAC TAAATTTTTC 
ATTGCAAATT ACAAAGATAG CGCGGATCAT TTAACGTTCG CCTATAAATA TGGAAGTTTT 
GTAAAGATTC AAGAATTTGT GGAGTTAAAA GAACGATTGG AAAATTGTTT TCAATTTGTC 
ATGACTACTT TAGATAAAAT GCTTTTGGAA TTAAGTTGGT GTAATAGCCC TACAACTTTG 
ATTTCTACGT TAAGTAATAT GGATATTCAA CTTAAGGAGG ATTCGATTCG TTTGAATTCG 
CTGCGGGACA ACCGTGATTT AGAGGTTGTT CGTGGGTGGG AACCACTCAC AGAAAATGGC 
GAAGATCTCA GAATGCAAGA AGAAACACGA ATTTGTATGT TAAGATTATT TATGGCGAGA 
AATTTAATAT TGAAAATTAT AGCAGCGTGT GTGGCACCCG ATAGTTCCTC TCTTCTTACC 
CGTTTTTCCG ACGAACTAAA GCAGTTGGAT AGCGAAGAAA TTCCTTCGAT TCTTCAAGAG 
TTTGAACCAG AAGGAAAGCA AAATAGGCCA TGTAAAATAC TAGTTCCTAT GGATGCAGTC 
GAAAGGTTAC GTGAAGCCCA TTATTCTGAA CAGTTGAAAA CAATTGCTCG ACTTGTTGAA 
TGTTTTGCTA ATTCGCGTTA CCCCGATTTT GAGTGCATTC AAATGTTACG TGCATCTCCA 
TGCCTTCAGA CAATAGATAT CCCGGAGAAG GGAATTCCAG TGTCTTTCAA GCATTTTTTA 
CTGAGAGCAT CCACGTGTAG TGAATCTCTT GCAATTATTA GTGCCATATG TACTTTATGG 
GCGATGCAAT TACAACCTCA GACTTCAGAG AAGAAAAATA AAAAGAAGTG TAACAAGCAA 
ATAGCACATA TTCCATTAAA TGATAATGAT CATAATTGGA AGGAAATTGC AATATTACTT 
TCAGAGAAAG TAGATAATTT AGATTCAGTA CTAACAGAAT TTGAAAAGTA TCAACTTAAC 
ACTGGACTAG ATAATGAAGA TGATGTTTGT GCTGCGATAC TCAAACATGG TCAAGCCAGT 
TTAGGACAAA GTTGCAGATC GTTAAAGTCT CGAGCTCAAA TCACGTTAAA GCTATTAAGC 
AGTTTAAAAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100882207 ( XM_012280699.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012136089.1 Megachile rotundata N-alpha-acetyltransferase 25, NatB auxiliary subunit (LOC100882207), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMe96752
Clone ID Related Accession (Same CDS sequence)	XM_012280699.1
Accession Version	XM_012280699.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	N-alpha-acetyltransferase 25, NatB auxiliary subunit isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797060.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAAA AGTTTGATTG GTTGGATAAT GGAAATAATA AAAAAGCTCT TCAAGAAGCA 
GATAAAGTGC TTAAAAAGCA ACCAACTAAT CAGTGTGCCA GGGTGCTCAA AGCTTTGGCC 
TTATTACGAC TTGGCAAAGA AAATGAATGT CAAACTATTA TGGATAAAGT ACGCTCAGAA 
ATTCCTTGCG AAGATTCTAC ACTGCAAGTT ATGAGTATTT GTTACAGAGA AAGACGTCAG 
CCTGATAAAA TTAGTGAAGT ATACGAAGCT GCAGCAAAAG CAGATCCTAA CAATGAAGAG 
TTATTAACAC ATCTTTTTAT GTCTTATGTT CGTCTTGGAG ACTATAAGAA ACAACAACAA 
ACGGCACTTG CCTTATATAA ATTGAAACCA AAAAATCCTT ATTATTTTTG GGCTGTGATG 
AGCATAGTTA TGCAAGCTAT ACATGCAGAT GAAAACTTAG CTAAAGGAAT TACTTTACCT 
TTAGCAGAAA GAATGGTACT AAAATTAGTT AAAGAAGGAA AAATGGAAGC AGAACAAGAA 
GTACAACTTT ATTTAATGAT ATTAGAACTT CAAGATAAAA GTGAAGAAAT GTTAAATGTT 
TTATCTGGAC CCTTAGCATC ACATCTTTCA TCTGTGTCCC AACGTAAGGC AGCACTTTTA 
TTAAAATTAG AACGTTTTCC TGAAGCTGCT AGTGCATATA AAGAACTTAT TAGTGAAAAT 
ACAGACAATT GGGCATATTA TAAAGACTAT CTCTTTGCTG CACTTAAATT TCAAAAACCA 
GAAGAATGTT TAAATTTTCT TAACAAAATT ATAACTATGT CGGAGAAAAC AGTAAGAGCT 
CCTTACCTTG CAAAATTCGA ATTATTAAAA CGTACTAAAA ACGGAGAAGT TATTGAAAAT 
TGTATTGAAC CAGTAGATTT AATGTATCAA TATTTCTCGA AATTTGGGGA AAAAGACTGT 
ATAGTTGGAG ATTTGCGACT TTATTTAGAT CTGTTAACTC CTACTGGAAA ACAACAGTTA 
CTTCAAAAAA TAGAGGAAGA TGTAGGTGTT AAGGCAGAAG GTTTTCCAAG CACTGTACAA 
CAAATGCAGA AACACATTCA CTTAGAACAA TTGCGACGCA TTTGTGGTTT CCATCATTCT 
CCAAATGCTG ATATAAACAA GCAAAAACAA TTGATAGAAC GATTATGTAA ATTATACGAA 
AAGGGCAATG AATTGTGTCC AATTCAAGAA AGATTACCAA CAGATTTTTG CCCTGCAGAT 
TCTTACATTC TTTTAGCTTC ACATTTATTA CATGAATTAT TTTATAATAC AAATGACGCA 
GTTTATCTTT ACAGGGCAAT GGCATTATTA GAACGTGGTT TATTATCGAG CCCTGCAAAT 
TTCCATATAA AAATTTTACT AGTACGAATA TATTTAGAAG CTGGATTAGT TGGGGCAGCC 
GATCATATTT TCACGTTATT GGACGTTAAA CATATTCAAT TAGATTCGTT GGGTTATTTA 
CATACACCAT TACTTGCTCC ACTTGGCCAC CTTTCGTTAG CTTCCATAAC TTTAGATCAT 
GCTACTAAAT TTTTCATTGC AAATTACAAA GATAGCGCGG ATCATTTAAC GTTCGCCTAT 
AAATATGGAA GTTTTGTAAA GATTCAAGAA TTTGTGGAGT TAAAAGAACG ATTGGAAAAT 
TGTTTTCAAT TTGTCATGAC TACTTTAGAT AAAATGCTTT TGGAATTAAG TTGGTGTAAT 
AGCCCTACAA CTTTGATTTC TACGTTAAGT AATATGGATA TTCAACTTAA GGAGGATTCG 
ATTCGTTTGA ATTCGCTGCG GGACAACCGT GATTTAGAGG TTGTTCGTGG GTGGGAACCA 
CTCACAGAAA ATGGCGAAGA TCTCAGAATG CAAGAAGAAA CACGAATTTG TATGTTAAGA 
TTATTTATGG CGAGAAATTT AATATTGAAA ATTATAGCAG CGTGTGTGGC ACCCGATAGT 
TCCTCTCTTC TTACCCGTTT TTCCGACGAA CTAAAGCAGT TGGATAGCGA AGAAATTCCT 
TCGATTCTTC AAGAGTTTGA ACCAGAAGGA AAGCAAAATA GGCCATGTAA AATACTAGTT 
CCTATGGATG CAGTCGAAAG GTTACGTGAA GCCCATTATT CTGAACAGTT GAAAACAATT 
GCTCGACTTG TTGAATGTTT TGCTAATTCG CGTTACCCCG ATTTTGAGTG CATTCAAATG 
TTACGTGCAT CTCCATGCCT TCAGACAATA GATATCCCGG AGAAGGGAAT TCCAGTGTCT 
TTCAAGCATT TTTTACTGAG AGCATCCACG TGTAGTGAAT CTCTTGCAAT TATTAGTGCC 
ATATGTACTT TATGGGCGAT GCAATTACAA CCTCAGACTT CAGAGAAGAA AAATAAAAAG 
AAGTGTAACA AGCAAATAGC ACATATTCCA TTAAATGATA ATGATCATAA TTGGAAGGAA 
ATTGCAATAT TACTTTCAGA GAAAGTAGAT AATTTAGATT CAGTACTAAC AGAATTTGAA 
AAGTATCAAC TTAACACTGG ACTAGATAAT GAAGATGATG TTTGTGCTGC GATACTCAAA 
CATGGTCAAG CCAGTTTAGG ACAAAGTTGC AGATCGTTAA AGTCTCGAGC TCAAATCACG 
TTAAAGCTAT TAAGCAGTTT AAAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012136089.1
CDS	213..2882
Translation	MKEKFDWLDNGNNKKALQEADKVLKKQPTNQCARVLKALALLRLGKENECQTIMDKVRSEIPCEDSTLQVMSICYRERRQPDKISEVYEAAAKADPNNEELLTHLFMSYVRLGDYKKQQQTALALYKLKPKNPYYFWAVMSIVMQAIHADENLAKGITLPLAERMVLKLVKEGKMEAEQEVQLYLMILELQDKSEEMLNVLSGPLASHLSSVSQRKAALLLKLERFPEAASAYKELISENTDNWAYYKDYLFAALKFQKPEECLNFLNKIITMSEKTVRAPYLAKFELLKRTKNGEVIENCIEPVDLMYQYFSKFGEKDCIVGDLRLYLDLLTPTGKQQLLQKIEEDVGVKAEGFPSTVQQMQKHIHLEQLRRICGFHHSPNADINKQKQLIERLCKLYEKGNELCPIQERLPTDFCPADSYILLASHLLHELFYNTNDAVYLYRAMALLERGLLSSPANFHIKILLVRIYLEAGLVGAADHIFTLLDVKHIQLDSLGYLHTPLLAPLGHLSLASITLDHATKFFIANYKDSADHLTFAYKYGSFVKIQEFVELKERLENCFQFVMTTLDKMLLELSWCNSPTTLISTLSNMDIQLKEDSIRLNSLRDNRDLEVVRGWEPLTENGEDLRMQEETRICMLRLFMARNLILKIIAACVAPDSSSLLTRFSDELKQLDSEEIPSILQEFEPEGKQNRPCKILVPMDAVERLREAHYSEQLKTIARLVECFANSRYPDFECIQMLRASPCLQTIDIPEKGIPVSFKHFLLRASTCSESLAIISAICTLWAMQLQPQTSEKKNKKKCNKQIAHIPLNDNDHNWKEIAILLSEKVDNLDSVLTEFEKYQLNTGLDNEDDVCAAILKHGQASLGQSCRSLKSRAQITLKLLSSLKS

Target ORF information:

RefSeq Version	XM_012280699.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata N-alpha-acetyltransferase 25, NatB auxiliary subunit (LOC100882207), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012280699.1

ORF Insert Sequence:

ATGAAGGAAA AGTTTGATTG GTTGGATAAT GGAAATAATA AAAAAGCTCT TCAAGAAGCA 
GATAAAGTGC TTAAAAAGCA ACCAACTAAT CAGTGTGCCA GGGTGCTCAA AGCTTTGGCC 
TTATTACGAC TTGGCAAAGA AAATGAATGT CAAACTATTA TGGATAAAGT ACGCTCAGAA 
ATTCCTTGCG AAGATTCTAC ACTGCAAGTT ATGAGTATTT GTTACAGAGA AAGACGTCAG 
CCTGATAAAA TTAGTGAAGT ATACGAAGCT GCAGCAAAAG CAGATCCTAA CAATGAAGAG 
TTATTAACAC ATCTTTTTAT GTCTTATGTT CGTCTTGGAG ACTATAAGAA ACAACAACAA 
ACGGCACTTG CCTTATATAA ATTGAAACCA AAAAATCCTT ATTATTTTTG GGCTGTGATG 
AGCATAGTTA TGCAAGCTAT ACATGCAGAT GAAAACTTAG CTAAAGGAAT TACTTTACCT 
TTAGCAGAAA GAATGGTACT AAAATTAGTT AAAGAAGGAA AAATGGAAGC AGAACAAGAA 
GTACAACTTT ATTTAATGAT ATTAGAACTT CAAGATAAAA GTGAAGAAAT GTTAAATGTT 
TTATCTGGAC CCTTAGCATC ACATCTTTCA TCTGTGTCCC AACGTAAGGC AGCACTTTTA 
TTAAAATTAG AACGTTTTCC TGAAGCTGCT AGTGCATATA AAGAACTTAT TAGTGAAAAT 
ACAGACAATT GGGCATATTA TAAAGACTAT CTCTTTGCTG CACTTAAATT TCAAAAACCA 
GAAGAATGTT TAAATTTTCT TAACAAAATT ATAACTATGT CGGAGAAAAC AGTAAGAGCT 
CCTTACCTTG CAAAATTCGA ATTATTAAAA CGTACTAAAA ACGGAGAAGT TATTGAAAAT 
TGTATTGAAC CAGTAGATTT AATGTATCAA TATTTCTCGA AATTTGGGGA AAAAGACTGT 
ATAGTTGGAG ATTTGCGACT TTATTTAGAT CTGTTAACTC CTACTGGAAA ACAACAGTTA 
CTTCAAAAAA TAGAGGAAGA TGTAGGTGTT AAGGCAGAAG GTTTTCCAAG CACTGTACAA 
CAAATGCAGA AACACATTCA CTTAGAACAA TTGCGACGCA TTTGTGGTTT CCATCATTCT 
CCAAATGCTG ATATAAACAA GCAAAAACAA TTGATAGAAC GATTATGTAA ATTATACGAA 
AAGGGCAATG AATTGTGTCC AATTCAAGAA AGATTACCAA CAGATTTTTG CCCTGCAGAT 
TCTTACATTC TTTTAGCTTC ACATTTATTA CATGAATTAT TTTATAATAC AAATGACGCA 
GTTTATCTTT ACAGGGCAAT GGCATTATTA GAACGTGGTT TATTATCGAG CCCTGCAAAT 
TTCCATATAA AAATTTTACT AGTACGAATA TATTTAGAAG CTGGATTAGT TGGGGCAGCC 
GATCATATTT TCACGTTATT GGACGTTAAA CATATTCAAT TAGATTCGTT GGGTTATTTA 
CATACACCAT TACTTGCTCC ACTTGGCCAC CTTTCGTTAG CTTCCATAAC TTTAGATCAT 
GCTACTAAAT TTTTCATTGC AAATTACAAA GATAGCGCGG ATCATTTAAC GTTCGCCTAT 
AAATATGGAA GTTTTGTAAA GATTCAAGAA TTTGTGGAGT TAAAAGAACG ATTGGAAAAT 
TGTTTTCAAT TTGTCATGAC TACTTTAGAT AAAATGCTTT TGGAATTAAG TTGGTGTAAT 
AGCCCTACAA CTTTGATTTC TACGTTAAGT AATATGGATA TTCAACTTAA GGAGGATTCG 
ATTCGTTTGA ATTCGCTGCG GGACAACCGT GATTTAGAGG TTGTTCGTGG GTGGGAACCA 
CTCACAGAAA ATGGCGAAGA TCTCAGAATG CAAGAAGAAA CACGAATTTG TATGTTAAGA 
TTATTTATGG CGAGAAATTT AATATTGAAA ATTATAGCAG CGTGTGTGGC ACCCGATAGT 
TCCTCTCTTC TTACCCGTTT TTCCGACGAA CTAAAGCAGT TGGATAGCGA AGAAATTCCT 
TCGATTCTTC AAGAGTTTGA ACCAGAAGGA AAGCAAAATA GGCCATGTAA AATACTAGTT 
CCTATGGATG CAGTCGAAAG GTTACGTGAA GCCCATTATT CTGAACAGTT GAAAACAATT 
GCTCGACTTG TTGAATGTTT TGCTAATTCG CGTTACCCCG ATTTTGAGTG CATTCAAATG 
TTACGTGCAT CTCCATGCCT TCAGACAATA GATATCCCGG AGAAGGGAAT TCCAGTGTCT 
TTCAAGCATT TTTTACTGAG AGCATCCACG TGTAGTGAAT CTCTTGCAAT TATTAGTGCC 
ATATGTACTT TATGGGCGAT GCAATTACAA CCTCAGACTT CAGAGAAGAA AAATAAAAAG 
AAGTGTAACA AGCAAATAGC ACATATTCCA TTAAATGATA ATGATCATAA TTGGAAGGAA 
ATTGCAATAT TACTTTCAGA GAAAGTAGAT AATTTAGATT CAGTACTAAC AGAATTTGAA 
AAGTATCAAC TTAACACTGG ACTAGATAAT GAAGATGATG TTTGTGCTGC GATACTCAAA 
CATGGTCAAG CCAGTTTAGG ACAAAGTTGC AGATCGTTAA AGTCTCGAGC TCAAATCACG 
TTAAAGCTAT TAAGCAGTTT AAAAAGCTGA