LOC100880945 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100880945
Entrez Gene ID	100880945
Full Name	uncharacterized LOC100880945
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003705459.2	XP_003705507.1	uncharacterized protein LOC100880945

Pathways from BioSystems
Gene Ontology

The following LOC100880945 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100880945 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe109108	XM_003705459.2 Latest version!	Megachile rotundata uncharacterized LOC100880945 (LOC100880945), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100880945 ( XM_003705459.2 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_003705507.1 Megachile rotundata uncharacterized LOC100880945 (LOC100880945), mRNA.

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CloneID	OMe109108
Clone ID Related Accession (Same CDS sequence)	XM_003705459.2
Accession Version	XM_003705459.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	uncharacterized protein LOC100880945
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797291.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2015 this sequence version replaced XM_003705459.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATTCGA GGACGCTGTT GATCGTCGCG TGTTTTTGCG TGGCACGCGC CAGCTCGCAG 
TTCTTCAGCT TCAAAAACCT GAAGGATTAC ACGAGGATCT TCCAGCTGGA CGGTCTGCTG 
AAGAACGAGA CCGGTAACGA GCTGTGGAGC GGCCTGTTCA GGGACTGTGA CCGATCAGTG 
ACGTTCTCGT GCATTCAGAA GAACGCGTAC TCGTATTTGG ACAACGTTTT TGTGGACCGG 
GACAATGTTA CCGTGTTCGA GGGGTTGACG CTGACGAAGA ACGACCTGGA TTACGGGACG 
TGTCGCAGGA ACTCGCTGGA AGAGAACCTG GTGGAAGGAT CAGTCAAGGA CGACTGCGAT 
GAAGAGGAAA GTGAAACAGG ACGAGAGTTC GAGGAGGAAC GATCGCCTTT GGAGGAAGTG 
ACCGATGCTT TGAGGAAGAG GGCGGTGAAG TTCATTGCTA CTCGGGATTA CGAGATCCAG 
CTGCCTGATT TCTTTTTTGA GGGGGCGGCG ATTAGGATTA GCCCTAAGGA GGTCGATGAG 
AATGGCGCTT TGGTCAGGGT GGACTTTGGA CAACGGGGGG TGCAGGGTGA AGGGAGGATC 
TTCAAGAAAA TTAGGAAGTT CATACAGAAC AAGTTGCTGA CGAGTTTGCT GGCACTGCTG 
CTCATTATCA AGCTGATAAA AGTGAAATTC ATGTTCGTGA TACCCTTCCT GTTCGGCGTG 
GGGACCGCCA AGAAGTTGTT CCTGAAACTG TTACTTTTCT TCATACCTGC ATTCGCGCAT 
ATCTTCAAAC TGTGCTCGAG CTATTACACG TCGCACGCGA CCAAATACCA TCATCATCAT 
CATCAGATAG CGCATCACCA TCACCACGTC CCAGTTCCCG TGCCAATACC CACATACTAC 
AACCACCACC ATCATCACCA CGACGAGGAG GACTTCGACG GCTACGACTA CGCTCATCCT 
CACATACAAT ATCGAAAAGA CGTTGAAGAG CTGAAGGAGT GGGGCATCGA GCCGTACGAA 
GAGTCTTACG AGCAGCACAA CGACCCTGGC CGAGTGCCAG GAGTGCTGCC AGCGCCGTTT 
CCAGGACCCA CGTACGCAGG AGCGTATGGT ATACCACAAT ATGCGCCCAA CGCTCAGAAT 
ATACCAACGA CATACACAGA GAAACGCCCG ATATCCGCCC ACAATTTGGC CTACTCCGCT 
TATGCCGATC GACGAACTCA AGCTTCCGTA CCTTCGAACT CCCCTAATCT ACAGTCGGCG 
ATACTATCAA AGTCTACGAA CCTCAAAACG TCCCACAACG TGGGTCAAAA CGTTAGACAG 
GTTCCCACAG GAACCTTGAA AGCACAGGAA GTAAAAATTG TACCCAAACA GACGATCAGT 
TACGATGACG AGTTTTATGG GCCAATCGTT GGTAGGCTAG AAGAGATATT CAGACAATTG 
AGATTCAACG AGGAGGTCTG CAGAGAGAGA TTGGTGTGCA GCATGTACAA GAACCCGACT 
GTTTACTCGC CACACAGTAA TCTCGTTTCC AACGAACTCT CCAGAGACCC ACAAGAGTTG 
AAGCGGACAG TTACCGAAAG CCCGTCCAGC CAGAAATTTC ACCGATATCT GAGCGCAGCG 
AGATTGGGTC AAGATGGAGG CGACTGCTTA AGAACTTATC CGTGTCACAT AAATACCGAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003705507.1
CDS	115..1797
Translation	MHSRTLLIVACFCVARASSQFFSFKNLKDYTRIFQLDGLLKNETGNELWSGLFRDCDRSVTFSCIQKNAYSYLDNVFVDRDNVTVFEGLTLTKNDLDYGTCRRNSLEENLVEGSVKDDCDEEESETGREFEEERSPLEEVTDALRKRAVKFIATRDYEIQLPDFFFEGAAIRISPKEVDENGALVRVDFGQRGVQGEGRIFKKIRKFIQNKLLTSLLALLLIIKLIKVKFMFVIPFLFGVGTAKKLFLKLLLFFIPAFAHIFKLCSSYYTSHATKYHHHHHQIAHHHHHVPVPVPIPTYYNHHHHHHDEEDFDGYDYAHPHIQYRKDVEELKEWGIEPYEESYEQHNDPGRVPGVLPAPFPGPTYAGAYGIPQYAPNAQNIPTTYTEKRPISAHNLAYSAYADRRTQASVPSNSPNLQSAILSKSTNLKTSHNVGQNVRQVPTGTLKAQEVKIVPKQTISYDDEFYGPIVGRLEEIFRQLRFNEEVCRERLVCSMYKNPTVYSPHSNLVSNELSRDPQELKRTVTESPSSQKFHRYLSAARLGQDGGDCLRTYPCHINTE

Target ORF information:

RefSeq Version	XM_003705459.2
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata uncharacterized LOC100880945 (LOC100880945), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003705459.2

ORF Insert Sequence:

ATGCATTCGA GGACGCTGTT GATCGTCGCG TGTTTTTGCG TGGCACGCGC CAGCTCGCAG 
TTCTTCAGCT TCAAAAACCT GAAGGATTAC ACGAGGATCT TCCAGCTGGA CGGTCTGCTG 
AAGAACGAGA CCGGTAACGA GCTGTGGAGC GGCCTGTTCA GGGACTGTGA CCGATCAGTG 
ACGTTCTCGT GCATTCAGAA GAACGCGTAC TCGTATTTGG ACAACGTTTT TGTGGACCGG 
GACAATGTTA CCGTGTTCGA GGGGTTGACG CTGACGAAGA ACGACCTGGA TTACGGGACG 
TGTCGCAGGA ACTCGCTGGA AGAGAACCTG GTGGAAGGAT CAGTCAAGGA CGACTGCGAT 
GAAGAGGAAA GTGAAACAGG ACGAGAGTTC GAGGAGGAAC GATCGCCTTT GGAGGAAGTG 
ACCGATGCTT TGAGGAAGAG GGCGGTGAAG TTCATTGCTA CTCGGGATTA CGAGATCCAG 
CTGCCTGATT TCTTTTTTGA GGGGGCGGCG ATTAGGATTA GCCCTAAGGA GGTCGATGAG 
AATGGCGCTT TGGTCAGGGT GGACTTTGGA CAACGGGGGG TGCAGGGTGA AGGGAGGATC 
TTCAAGAAAA TTAGGAAGTT CATACAGAAC AAGTTGCTGA CGAGTTTGCT GGCACTGCTG 
CTCATTATCA AGCTGATAAA AGTGAAATTC ATGTTCGTGA TACCCTTCCT GTTCGGCGTG 
GGGACCGCCA AGAAGTTGTT CCTGAAACTG TTACTTTTCT TCATACCTGC ATTCGCGCAT 
ATCTTCAAAC TGTGCTCGAG CTATTACACG TCGCACGCGA CCAAATACCA TCATCATCAT 
CATCAGATAG CGCATCACCA TCACCACGTC CCAGTTCCCG TGCCAATACC CACATACTAC 
AACCACCACC ATCATCACCA CGACGAGGAG GACTTCGACG GCTACGACTA CGCTCATCCT 
CACATACAAT ATCGAAAAGA CGTTGAAGAG CTGAAGGAGT GGGGCATCGA GCCGTACGAA 
GAGTCTTACG AGCAGCACAA CGACCCTGGC CGAGTGCCAG GAGTGCTGCC AGCGCCGTTT 
CCAGGACCCA CGTACGCAGG AGCGTATGGT ATACCACAAT ATGCGCCCAA CGCTCAGAAT 
ATACCAACGA CATACACAGA GAAACGCCCG ATATCCGCCC ACAATTTGGC CTACTCCGCT 
TATGCCGATC GACGAACTCA AGCTTCCGTA CCTTCGAACT CCCCTAATCT ACAGTCGGCG 
ATACTATCAA AGTCTACGAA CCTCAAAACG TCCCACAACG TGGGTCAAAA CGTTAGACAG 
GTTCCCACAG GAACCTTGAA AGCACAGGAA GTAAAAATTG TACCCAAACA GACGATCAGT 
TACGATGACG AGTTTTATGG GCCAATCGTT GGTAGGCTAG AAGAGATATT CAGACAATTG 
AGATTCAACG AGGAGGTCTG CAGAGAGAGA TTGGTGTGCA GCATGTACAA GAACCCGACT 
GTTTACTCGC CACACAGTAA TCTCGTTTCC AACGAACTCT CCAGAGACCC ACAAGAGTTG 
AAGCGGACAG TTACCGAAAG CCCGTCCAGC CAGAAATTTC ACCGATATCT GAGCGCAGCG 
AGATTGGGTC AAGATGGAGG CGACTGCTTA AGAACTTATC CGTGTCACAT AAATACCGAG 
TAA