LOC100880727 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100880727
Entrez Gene ID	100880727
Full Name	metal transporter CNNM4
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012291487.1	XP_012146877.1	metal transporter CNNM4 isoform X2
XM_012291486.1	XP_012146876.1	metal transporter CNNM4 isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC100880727 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100880727 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe110059	XM_012291486.1 Latest version!	Megachile rotundata metal transporter CNNM4 (LOC100880727), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OMe110060	XM_012291487.1 Latest version!	Megachile rotundata metal transporter CNNM4 (LOC100880727), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100880727 ( XM_012291486.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012146876.1 Megachile rotundata metal transporter CNNM4 (LOC100880727), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMe110059
Clone ID Related Accession (Same CDS sequence)	XM_012291486.1
Accession Version	XM_012291486.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2991bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	metal transporter CNNM4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797353.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTGT CACAGTGCAT TTGTTCCATG ATGGCGCAAC ATGTTATTCA TTCAAGCACA 
CCGGCAACTC TTTTGTTACT TGTTTTTACG ATATCCAACT TAGTAACTAG CAATGCATTT 
ACAGACGTGC AAGTATTTGA TAACCTGAAA GTGAAATCAG TGAATGCTTT AATTACAAAG 
CAATCTCATG CACGAGAGTA CTTATCTGGT GCTCCAGGTT TCTTTGTAGA GATTCTTGGC 
ACCGGCCTGG AGAGAAATGT TTCGTTGCGC TGGTCAGCTA CACTCGACAA TTGTGATCCA 
AACAACAAGT TGAATGCAAT TTGGATCGAT CATAATGGCA ATCGTGCGAT ATATAAGTTT 
ATCAAAATTC CTAATTTTAA GGTTACCACG ATCTATTTCT GTCTGAGAAC CGTCAGTAGG 
TTCGGTGAAA CATGGACCAA TCTTGGAAGC AATTTAACTA TTCAGCGCCC AATTCATTCA 
GAAAAATATT TTCGTACGCG TCGTGCCAAT TCATTAGCTA GCGTTGATGG TGATTACAAA 
AATCCATCAA CTGAGCAAAT CCGTACAGAT GTATTTATTG AGGGATTAAG AATAGAAACA 
TCAGCAAAGG ATCCTAGCTA CAGCGAAGAT GGTATTCCAG AGATATTAGC TGGAAGCAGA 
GCAGTTATAC GATTGTTTGG TTTTGGCTTT ACAGAAGATA CTTTGGTTAC TTTCACAGAT 
GTACCAGCAA AAAGGGGTAC TATTTGTGAC AAAATTAAAA GCAAGGAATT TCCAGTACAA 
CGTATAAACA ATCATACTGC AATTATCGAT GTTGTATTAC CACTTGGTTC ACCATTTTAT 
GTGTGTGCAA AACAACCAGT GTATGGAAAA GAAGGTCCTG ACCTTTTGCT GTTTAAACAT 
CAAGGTACTG AAACATGGAA TACTATATAT ACATACGAGA AATTTTTACC ACTTTGGCTT 
AGTATTGTAA TTATTCTCAT GTGTCTAAGC TTTTCTGCTT TGTTCTCTGG TTTAAATTTA 
GGGTTAATGG CAATGGATAG AACTGAATTG AAAATACTTT GTAATACTGG AACAGAGAAG 
GAAAAACAAT ATGCAAGAAC AATACAACCA GTGAGAAATC ATGGGAATTA TTTGTTATGT 
TCGATTTTGT TCTCAAATGT TCTGGTAAAT TCAATTTTTA CCATTTTATT AGATGATCTA 
ACTTCTGGAT TAGTTGCCGT TATTTGTTCT ACCTTAGCTA TTGTAATATT TGGTGAAATT 
TCACCGCAAG CTATTTGTAG CAGACATGGA TTGTGTATTG GGGCAAAGAC TATTTACGTT 
ACAAAATTAA CCATGATAAT AACATTTCCA TTGAGCTATC CTATTAGCAA GCTCTTAGAT 
GTTCTTCTTG GTGAAGAAAT TGGCAATGTA TATAATCGTG AACGATTGAA AGAACTTGTA 
AAGGTAACAA CAGGGTATAA TGATTTAGAA AAGGATGAAG TGAATATTAT TGCTGGAGCA 
TTAGAATTGC GAAAAAAGAC CGTGAAAGAT GTAATGACAA AAATCGAAGA TGTATACATG 
TTGAATTATG ACGCTATTTT AGATTTTGAA ACAGTATCGG AAATCATGAA ATCAGGCTTT 
TCGCGTATAC CAGTTTACGA GGGTACCAGA ACAAACATAG TTACAATGCT TTATATTAAA 
GATCTTGCTT TCGTTGATCC AGATGATAAT ATGCCACTTA AAACTTTGTG CCAATTTTAT 
CAAAATCCAT GTAACTTCAT TTTCGAAGAT GTTACGTTAG ATATTATGTT TAAACAATTC 
AAAGAAGGCC ATAAAGGTCA TATGGCATTT GTGCAAAGAG TAAATAATGA AGGCGAAGGC 
GATCCATTTT ATGAGGTAAT AGGTTTAGTC ACATTGGAAG ATGTTATAGA AGAATTAATT 
CAAGCAGAGA TTATGGATGA AACTGATGTA TTTACGGATA ATAGAACTAA ACGTAGAAGG 
CAAGCAAGGC ATAAAATACA AGATTTTACT GTGTTTGCGG AGAAGAAAGA AAATCAACGT 
ATTCATATAT CGCCGCAGTT GACATTGGCC ATGTTTCAGT ACTTATCTAC AACTGTTGAT 
GCATTTAAAC CCGATACAAT ATCAGAAACT ATTTTGCGTC GTTTACTTAA ACAAGATATT 
ATTTATCATA TCAAAGTGAA GTCACGCGAA AAAGCAAGAA ATGATCCGAC GGCAGTAATT 
TATCAACAAG GAAAAGCAGT TGATTATTTT GTCCTAATCT TAGAAGGTCG AGTTGAAGTT 
ACGGTCGGCA AAGAAAACAT GATGTTCGAA AGCGGACCAT TTACATATTT TGGTTCTCAG 
GCACTCACTG TTAACGTTGG AATAGCTGAG TCACCTACCG CCACAAATCC CCAAACAGTC 
GGCAGCATAC AATCGATTAA TCTAGATTCC ATGTTACGAT ATACGTTTGT TCCTGATTAT 
ACAGTGCGAG CGGTAACCGA AGTATTTTAT GTTAAAATTA AAAGATCTTT GTACTTAGCT 
GCGAAACGGG CTACTCTTTT GGAACGAAGC CAAAAAGACC CGATTCCCGG AAGCCAAGAG 
CAATTTGACG ACGAGATAGA AAAGCTACTG CATTCTTTGG ACGAGGATGA TCGTAGTGTA 
CCAGAATCTC CAGTATTACC AATTGATAAA GATAAGGAAA GTAAGAAAGA AAAAGAAAAA 
GATTCTGTAC AATCTCCAAT TCATAGTGCT ACAGCTCCAA CTTCACCTGC TCCAGTTAGT 
GCTAGTCCAG TTACACATTC AAAGTTTATT TCATCGTACA GCGATCATAA TGGCAAATTA 
AAAAATTCGC CAGTAAAGGG AACGGTCACG AGTCCGAATC AAGGACAAAC CAATTTAGAT 
TTAAATTCAG ACATTCTTGC TCGTCATGAA GCTAATAAAA TCATATCAGC AAAAAAATTA 
ATTGAAGAGG AAGAAAGAAC GAATCTCCTG CCTAAGCAAG AAGACTCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012146876.1
CDS	132..3122
Translation	MKLSQCICSMMAQHVIHSSTPATLLLLVFTISNLVTSNAFTDVQVFDNLKVKSVNALITKQSHAREYLSGAPGFFVEILGTGLERNVSLRWSATLDNCDPNNKLNAIWIDHNGNRAIYKFIKIPNFKVTTIYFCLRTVSRFGETWTNLGSNLTIQRPIHSEKYFRTRRANSLASVDGDYKNPSTEQIRTDVFIEGLRIETSAKDPSYSEDGIPEILAGSRAVIRLFGFGFTEDTLVTFTDVPAKRGTICDKIKSKEFPVQRINNHTAIIDVVLPLGSPFYVCAKQPVYGKEGPDLLLFKHQGTETWNTIYTYEKFLPLWLSIVIILMCLSFSALFSGLNLGLMAMDRTELKILCNTGTEKEKQYARTIQPVRNHGNYLLCSILFSNVLVNSIFTILLDDLTSGLVAVICSTLAIVIFGEISPQAICSRHGLCIGAKTIYVTKLTMIITFPLSYPISKLLDVLLGEEIGNVYNRERLKELVKVTTGYNDLEKDEVNIIAGALELRKKTVKDVMTKIEDVYMLNYDAILDFETVSEIMKSGFSRIPVYEGTRTNIVTMLYIKDLAFVDPDDNMPLKTLCQFYQNPCNFIFEDVTLDIMFKQFKEGHKGHMAFVQRVNNEGEGDPFYEVIGLVTLEDVIEELIQAEIMDETDVFTDNRTKRRRQARHKIQDFTVFAEKKENQRIHISPQLTLAMFQYLSTTVDAFKPDTISETILRRLLKQDIIYHIKVKSREKARNDPTAVIYQQGKAVDYFVLILEGRVEVTVGKENMMFESGPFTYFGSQALTVNVGIAESPTATNPQTVGSIQSINLDSMLRYTFVPDYTVRAVTEVFYVKIKRSLYLAAKRATLLERSQKDPIPGSQEQFDDEIEKLLHSLDEDDRSVPESPVLPIDKDKESKKEKEKDSVQSPIHSATAPTSPAPVSASPVTHSKFISSYSDHNGKLKNSPVKGTVTSPNQGQTNLDLNSDILARHEANKIISAKKLIEEEERTNLLPKQEDS

Target ORF information:

RefSeq Version	XM_012291486.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata metal transporter CNNM4 (LOC100880727), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012291486.1

ORF Insert Sequence:

ATGAAGTTGT CACAGTGCAT TTGTTCCATG ATGGCGCAAC ATGTTATTCA TTCAAGCACA 
CCGGCAACTC TTTTGTTACT TGTTTTTACG ATATCCAACT TAGTAACTAG CAATGCATTT 
ACAGACGTGC AAGTATTTGA TAACCTGAAA GTGAAATCAG TGAATGCTTT AATTACAAAG 
CAATCTCATG CACGAGAGTA CTTATCTGGT GCTCCAGGTT TCTTTGTAGA GATTCTTGGC 
ACCGGCCTGG AGAGAAATGT TTCGTTGCGC TGGTCAGCTA CACTCGACAA TTGTGATCCA 
AACAACAAGT TGAATGCAAT TTGGATCGAT CATAATGGCA ATCGTGCGAT ATATAAGTTT 
ATCAAAATTC CTAATTTTAA GGTTACCACG ATCTATTTCT GTCTGAGAAC CGTCAGTAGG 
TTCGGTGAAA CATGGACCAA TCTTGGAAGC AATTTAACTA TTCAGCGCCC AATTCATTCA 
GAAAAATATT TTCGTACGCG TCGTGCCAAT TCATTAGCTA GCGTTGATGG TGATTACAAA 
AATCCATCAA CTGAGCAAAT CCGTACAGAT GTATTTATTG AGGGATTAAG AATAGAAACA 
TCAGCAAAGG ATCCTAGCTA CAGCGAAGAT GGTATTCCAG AGATATTAGC TGGAAGCAGA 
GCAGTTATAC GATTGTTTGG TTTTGGCTTT ACAGAAGATA CTTTGGTTAC TTTCACAGAT 
GTACCAGCAA AAAGGGGTAC TATTTGTGAC AAAATTAAAA GCAAGGAATT TCCAGTACAA 
CGTATAAACA ATCATACTGC AATTATCGAT GTTGTATTAC CACTTGGTTC ACCATTTTAT 
GTGTGTGCAA AACAACCAGT GTATGGAAAA GAAGGTCCTG ACCTTTTGCT GTTTAAACAT 
CAAGGTACTG AAACATGGAA TACTATATAT ACATACGAGA AATTTTTACC ACTTTGGCTT 
AGTATTGTAA TTATTCTCAT GTGTCTAAGC TTTTCTGCTT TGTTCTCTGG TTTAAATTTA 
GGGTTAATGG CAATGGATAG AACTGAATTG AAAATACTTT GTAATACTGG AACAGAGAAG 
GAAAAACAAT ATGCAAGAAC AATACAACCA GTGAGAAATC ATGGGAATTA TTTGTTATGT 
TCGATTTTGT TCTCAAATGT TCTGGTAAAT TCAATTTTTA CCATTTTATT AGATGATCTA 
ACTTCTGGAT TAGTTGCCGT TATTTGTTCT ACCTTAGCTA TTGTAATATT TGGTGAAATT 
TCACCGCAAG CTATTTGTAG CAGACATGGA TTGTGTATTG GGGCAAAGAC TATTTACGTT 
ACAAAATTAA CCATGATAAT AACATTTCCA TTGAGCTATC CTATTAGCAA GCTCTTAGAT 
GTTCTTCTTG GTGAAGAAAT TGGCAATGTA TATAATCGTG AACGATTGAA AGAACTTGTA 
AAGGTAACAA CAGGGTATAA TGATTTAGAA AAGGATGAAG TGAATATTAT TGCTGGAGCA 
TTAGAATTGC GAAAAAAGAC CGTGAAAGAT GTAATGACAA AAATCGAAGA TGTATACATG 
TTGAATTATG ACGCTATTTT AGATTTTGAA ACAGTATCGG AAATCATGAA ATCAGGCTTT 
TCGCGTATAC CAGTTTACGA GGGTACCAGA ACAAACATAG TTACAATGCT TTATATTAAA 
GATCTTGCTT TCGTTGATCC AGATGATAAT ATGCCACTTA AAACTTTGTG CCAATTTTAT 
CAAAATCCAT GTAACTTCAT TTTCGAAGAT GTTACGTTAG ATATTATGTT TAAACAATTC 
AAAGAAGGCC ATAAAGGTCA TATGGCATTT GTGCAAAGAG TAAATAATGA AGGCGAAGGC 
GATCCATTTT ATGAGGTAAT AGGTTTAGTC ACATTGGAAG ATGTTATAGA AGAATTAATT 
CAAGCAGAGA TTATGGATGA AACTGATGTA TTTACGGATA ATAGAACTAA ACGTAGAAGG 
CAAGCAAGGC ATAAAATACA AGATTTTACT GTGTTTGCGG AGAAGAAAGA AAATCAACGT 
ATTCATATAT CGCCGCAGTT GACATTGGCC ATGTTTCAGT ACTTATCTAC AACTGTTGAT 
GCATTTAAAC CCGATACAAT ATCAGAAACT ATTTTGCGTC GTTTACTTAA ACAAGATATT 
ATTTATCATA TCAAAGTGAA GTCACGCGAA AAAGCAAGAA ATGATCCGAC GGCAGTAATT 
TATCAACAAG GAAAAGCAGT TGATTATTTT GTCCTAATCT TAGAAGGTCG AGTTGAAGTT 
ACGGTCGGCA AAGAAAACAT GATGTTCGAA AGCGGACCAT TTACATATTT TGGTTCTCAG 
GCACTCACTG TTAACGTTGG AATAGCTGAG TCACCTACCG CCACAAATCC CCAAACAGTC 
GGCAGCATAC AATCGATTAA TCTAGATTCC ATGTTACGAT ATACGTTTGT TCCTGATTAT 
ACAGTGCGAG CGGTAACCGA AGTATTTTAT GTTAAAATTA AAAGATCTTT GTACTTAGCT 
GCGAAACGGG CTACTCTTTT GGAACGAAGC CAAAAAGACC CGATTCCCGG AAGCCAAGAG 
CAATTTGACG ACGAGATAGA AAAGCTACTG CATTCTTTGG ACGAGGATGA TCGTAGTGTA 
CCAGAATCTC CAGTATTACC AATTGATAAA GATAAGGAAA GTAAGAAAGA AAAAGAAAAA 
GATTCTGTAC AATCTCCAAT TCATAGTGCT ACAGCTCCAA CTTCACCTGC TCCAGTTAGT 
GCTAGTCCAG TTACACATTC AAAGTTTATT TCATCGTACA GCGATCATAA TGGCAAATTA 
AAAAATTCGC CAGTAAAGGG AACGGTCACG AGTCCGAATC AAGGACAAAC CAATTTAGAT 
TTAAATTCAG ACATTCTTGC TCGTCATGAA GCTAATAAAA TCATATCAGC AAAAAAATTA 
ATTGAAGAGG AAGAAAGAAC GAATCTCCTG CCTAAGCAAG AAGACTCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100880727 ( XM_012291487.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012146877.1 Megachile rotundata metal transporter CNNM4 (LOC100880727), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMe110060
Clone ID Related Accession (Same CDS sequence)	XM_012291487.1
Accession Version	XM_012291487.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	metal transporter CNNM4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797353.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTGT CACAGTGCAT TTGTTCCATG ATGGCGCAAC ATGTTATTCA TTCAAGCACA 
CCGGCAACTC TTTTGTTACT TGTTTTTACG ATATCCAACT TAGTAACTAG CAATGCATTT 
ACAGACGTGC AAGTATTTGA TAACCTGAAA GTGAAATCAG TGAATGCTTT AATTACAAAG 
CAATCTCATG CACGAGAGTA CTTATCTGGT GCTCCAGGTT TCTTTGTAGA GATTCTTGGC 
ACCGGCCTGG AGAGAAATGT TTCGTTGCGC TGGTCAGCTA CACTCGACAA TTGTGATCCA 
AACAACAAGT TGAATGCAAT TTGGATCGAT CATAATGGCA ATCGTGCGAT ATATAAGTTT 
ATCAAAATTC CTAATTTTAA GGTTACCACG ATCTATTTCT GTCTGAGAAC CGTCAGTAGG 
TTCGGTGAAA CATGGACCAA TCTTGGAAGC AATTTAACTA TTCAGCGCCC AATTCATTCA 
GAAAAATATT TTCGTACGCG TCGTGCCAAT TCATTAGCTA GCGTTGATGG TGATTACAAA 
AATCCATCAA CTGAGCAAAT CCGTACAGAT GTATTTATTG AGGGATTAAG AATAGAAACA 
TCAGCAAAGG ATCCTAGCTA CAGCGAAGAT GGTATTCCAG AGATATTAGC TGGAAGCAGA 
GCAGTTATAC GATTGTTTGG TTTTGGCTTT ACAGAAGATA CTTTGGTTAC TTTCACAGAT 
GTACCAGCAA AAAGGGGTAC TATTTGTGAC AAAATTAAAA GCAAGGAATT TCCAGTACAA 
CGTATAAACA ATCATACTGC AATTATCGAT GTTGTATTAC CACTTGGTTC ACCATTTTAT 
GTGTGTGCAA AACAACCAGT GTATGGAAAA GAAGGTCCTG ACCTTTTGCT GTTTAAACAT 
CAAGGGTTAA TGGCAATGGA TAGAACTGAA TTGAAAATAC TTTGTAATAC TGGAACAGAG 
AAGGAAAAAC AATATGCAAG AACAATACAA CCAGTGAGAA ATCATGGGAA TTATTTGTTA 
TGTTCGATTT TGTTCTCAAA TGTTCTGGTA AATTCAATTT TTACCATTTT ATTAGATGAT 
CTAACTTCTG GATTAGTTGC CGTTATTTGT TCTACCTTAG CTATTGTAAT ATTTGGTGAA 
ATTTCACCGC AAGCTATTTG TAGCAGACAT GGATTGTGTA TTGGGGCAAA GACTATTTAC 
GTTACAAAAT TAACCATGAT AATAACATTT CCATTGAGCT ATCCTATTAG CAAGCTCTTA 
GATGTTCTTC TTGGTGAAGA AATTGGCAAT GTATATAATC GTGAACGATT GAAAGAACTT 
GTAAAGGTAA CAACAGGGTA TAATGATTTA GAAAAGGATG AAGTGAATAT TATTGCTGGA 
GCATTAGAAT TGCGAAAAAA GACCGTGAAA GATGTAATGA CAAAAATCGA AGATGTATAC 
ATGTTGAATT ATGACGCTAT TTTAGATTTT GAAACAGTAT CGGAAATCAT GAAATCAGGC 
TTTTCGCGTA TACCAGTTTA CGAGGGTACC AGAACAAACA TAGTTACAAT GCTTTATATT 
AAAGATCTTG CTTTCGTTGA TCCAGATGAT AATATGCCAC TTAAAACTTT GTGCCAATTT 
TATCAAAATC CATGTAACTT CATTTTCGAA GATGTTACGT TAGATATTAT GTTTAAACAA 
TTCAAAGAAG GCCATAAAGG TCATATGGCA TTTGTGCAAA GAGTAAATAA TGAAGGCGAA 
GGCGATCCAT TTTATGAGGT AATAGGTTTA GTCACATTGG AAGATGTTAT AGAAGAATTA 
ATTCAAGCAG AGATTATGGA TGAAACTGAT GTATTTACGG ATAATAGAAC TAAACGTAGA 
AGGCAAGCAA GGCATAAAAT ACAAGATTTT ACTGTGTTTG CGGAGAAGAA AGAAAATCAA 
CGTATTCATA TATCGCCGCA GTTGACATTG GCCATGTTTC AGTACTTATC TACAACTGTT 
GATGCATTTA AACCCGATAC AATATCAGAA ACTATTTTGC GTCGTTTACT TAAACAAGAT 
ATTATTTATC ATATCAAAGT GAAGTCACGC GAAAAAGCAA GAAATGATCC GACGGCAGTA 
ATTTATCAAC AAGGAAAAGC AGTTGATTAT TTTGTCCTAA TCTTAGAAGG TCGAGTTGAA 
GTTACGGTCG GCAAAGAAAA CATGATGTTC GAAAGCGGAC CATTTACATA TTTTGGTTCT 
CAGGCACTCA CTGTTAACGT TGGAATAGCT GAGTCACCTA CCGCCACAAA TCCCCAAACA 
GTCGGCAGCA TACAATCGAT TAATCTAGAT TCCATGTTAC GATATACGTT TGTTCCTGAT 
TATACAGTGC GAGCGGTAAC CGAAGTATTT TATGTTAAAA TTAAAAGATC TTTGTACTTA 
GCTGCGAAAC GGGCTACTCT TTTGGAACGA AGCCAAAAAG ACCCGATTCC CGGAAGCCAA 
GAGCAATTTG ACGACGAGAT AGAAAAGCTA CTGCATTCTT TGGACGAGGA TGATCGTAGT 
GTACCAGAAT CTCCAGTATT ACCAATTGAT AAAGATAAGG AAAGTAAGAA AGAAAAAGAA 
AAAGATTCTG TACAATCTCC AATTCATAGT GCTACAGCTC CAACTTCACC TGCTCCAGTT 
AGTGCTAGTC CAGTTACACA TTCAAAGTTT ATTTCATCGT ACAGCGATCA TAATGGCAAA 
TTAAAAAATT CGCCAGTAAA GGGAACGGTC ACGAGTCCGA ATCAAGGACA AACCAATTTA 
GATTTAAATT CAGACATTCT TGCTCGTCAT GAAGCTAATA AAATCATATC AGCAAAAAAA 
TTAATTGAAG AGGAAGAAAG AACGAATCTC CTGCCTAAGC AAGAAGACTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012146877.1
CDS	134..3007
Translation	MKLSQCICSMMAQHVIHSSTPATLLLLVFTISNLVTSNAFTDVQVFDNLKVKSVNALITKQSHAREYLSGAPGFFVEILGTGLERNVSLRWSATLDNCDPNNKLNAIWIDHNGNRAIYKFIKIPNFKVTTIYFCLRTVSRFGETWTNLGSNLTIQRPIHSEKYFRTRRANSLASVDGDYKNPSTEQIRTDVFIEGLRIETSAKDPSYSEDGIPEILAGSRAVIRLFGFGFTEDTLVTFTDVPAKRGTICDKIKSKEFPVQRINNHTAIIDVVLPLGSPFYVCAKQPVYGKEGPDLLLFKHQGLMAMDRTELKILCNTGTEKEKQYARTIQPVRNHGNYLLCSILFSNVLVNSIFTILLDDLTSGLVAVICSTLAIVIFGEISPQAICSRHGLCIGAKTIYVTKLTMIITFPLSYPISKLLDVLLGEEIGNVYNRERLKELVKVTTGYNDLEKDEVNIIAGALELRKKTVKDVMTKIEDVYMLNYDAILDFETVSEIMKSGFSRIPVYEGTRTNIVTMLYIKDLAFVDPDDNMPLKTLCQFYQNPCNFIFEDVTLDIMFKQFKEGHKGHMAFVQRVNNEGEGDPFYEVIGLVTLEDVIEELIQAEIMDETDVFTDNRTKRRRQARHKIQDFTVFAEKKENQRIHISPQLTLAMFQYLSTTVDAFKPDTISETILRRLLKQDIIYHIKVKSREKARNDPTAVIYQQGKAVDYFVLILEGRVEVTVGKENMMFESGPFTYFGSQALTVNVGIAESPTATNPQTVGSIQSINLDSMLRYTFVPDYTVRAVTEVFYVKIKRSLYLAAKRATLLERSQKDPIPGSQEQFDDEIEKLLHSLDEDDRSVPESPVLPIDKDKESKKEKEKDSVQSPIHSATAPTSPAPVSASPVTHSKFISSYSDHNGKLKNSPVKGTVTSPNQGQTNLDLNSDILARHEANKIISAKKLIEEEERTNLLPKQEDS

Target ORF information:

RefSeq Version	XM_012291487.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata metal transporter CNNM4 (LOC100880727), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012291487.1

ORF Insert Sequence:

ATGAAGTTGT CACAGTGCAT TTGTTCCATG ATGGCGCAAC ATGTTATTCA TTCAAGCACA 
CCGGCAACTC TTTTGTTACT TGTTTTTACG ATATCCAACT TAGTAACTAG CAATGCATTT 
ACAGACGTGC AAGTATTTGA TAACCTGAAA GTGAAATCAG TGAATGCTTT AATTACAAAG 
CAATCTCATG CACGAGAGTA CTTATCTGGT GCTCCAGGTT TCTTTGTAGA GATTCTTGGC 
ACCGGCCTGG AGAGAAATGT TTCGTTGCGC TGGTCAGCTA CACTCGACAA TTGTGATCCA 
AACAACAAGT TGAATGCAAT TTGGATCGAT CATAATGGCA ATCGTGCGAT ATATAAGTTT 
ATCAAAATTC CTAATTTTAA GGTTACCACG ATCTATTTCT GTCTGAGAAC CGTCAGTAGG 
TTCGGTGAAA CATGGACCAA TCTTGGAAGC AATTTAACTA TTCAGCGCCC AATTCATTCA 
GAAAAATATT TTCGTACGCG TCGTGCCAAT TCATTAGCTA GCGTTGATGG TGATTACAAA 
AATCCATCAA CTGAGCAAAT CCGTACAGAT GTATTTATTG AGGGATTAAG AATAGAAACA 
TCAGCAAAGG ATCCTAGCTA CAGCGAAGAT GGTATTCCAG AGATATTAGC TGGAAGCAGA 
GCAGTTATAC GATTGTTTGG TTTTGGCTTT ACAGAAGATA CTTTGGTTAC TTTCACAGAT 
GTACCAGCAA AAAGGGGTAC TATTTGTGAC AAAATTAAAA GCAAGGAATT TCCAGTACAA 
CGTATAAACA ATCATACTGC AATTATCGAT GTTGTATTAC CACTTGGTTC ACCATTTTAT 
GTGTGTGCAA AACAACCAGT GTATGGAAAA GAAGGTCCTG ACCTTTTGCT GTTTAAACAT 
CAAGGGTTAA TGGCAATGGA TAGAACTGAA TTGAAAATAC TTTGTAATAC TGGAACAGAG 
AAGGAAAAAC AATATGCAAG AACAATACAA CCAGTGAGAA ATCATGGGAA TTATTTGTTA 
TGTTCGATTT TGTTCTCAAA TGTTCTGGTA AATTCAATTT TTACCATTTT ATTAGATGAT 
CTAACTTCTG GATTAGTTGC CGTTATTTGT TCTACCTTAG CTATTGTAAT ATTTGGTGAA 
ATTTCACCGC AAGCTATTTG TAGCAGACAT GGATTGTGTA TTGGGGCAAA GACTATTTAC 
GTTACAAAAT TAACCATGAT AATAACATTT CCATTGAGCT ATCCTATTAG CAAGCTCTTA 
GATGTTCTTC TTGGTGAAGA AATTGGCAAT GTATATAATC GTGAACGATT GAAAGAACTT 
GTAAAGGTAA CAACAGGGTA TAATGATTTA GAAAAGGATG AAGTGAATAT TATTGCTGGA 
GCATTAGAAT TGCGAAAAAA GACCGTGAAA GATGTAATGA CAAAAATCGA AGATGTATAC 
ATGTTGAATT ATGACGCTAT TTTAGATTTT GAAACAGTAT CGGAAATCAT GAAATCAGGC 
TTTTCGCGTA TACCAGTTTA CGAGGGTACC AGAACAAACA TAGTTACAAT GCTTTATATT 
AAAGATCTTG CTTTCGTTGA TCCAGATGAT AATATGCCAC TTAAAACTTT GTGCCAATTT 
TATCAAAATC CATGTAACTT CATTTTCGAA GATGTTACGT TAGATATTAT GTTTAAACAA 
TTCAAAGAAG GCCATAAAGG TCATATGGCA TTTGTGCAAA GAGTAAATAA TGAAGGCGAA 
GGCGATCCAT TTTATGAGGT AATAGGTTTA GTCACATTGG AAGATGTTAT AGAAGAATTA 
ATTCAAGCAG AGATTATGGA TGAAACTGAT GTATTTACGG ATAATAGAAC TAAACGTAGA 
AGGCAAGCAA GGCATAAAAT ACAAGATTTT ACTGTGTTTG CGGAGAAGAA AGAAAATCAA 
CGTATTCATA TATCGCCGCA GTTGACATTG GCCATGTTTC AGTACTTATC TACAACTGTT 
GATGCATTTA AACCCGATAC AATATCAGAA ACTATTTTGC GTCGTTTACT TAAACAAGAT 
ATTATTTATC ATATCAAAGT GAAGTCACGC GAAAAAGCAA GAAATGATCC GACGGCAGTA 
ATTTATCAAC AAGGAAAAGC AGTTGATTAT TTTGTCCTAA TCTTAGAAGG TCGAGTTGAA 
GTTACGGTCG GCAAAGAAAA CATGATGTTC GAAAGCGGAC CATTTACATA TTTTGGTTCT 
CAGGCACTCA CTGTTAACGT TGGAATAGCT GAGTCACCTA CCGCCACAAA TCCCCAAACA 
GTCGGCAGCA TACAATCGAT TAATCTAGAT TCCATGTTAC GATATACGTT TGTTCCTGAT 
TATACAGTGC GAGCGGTAAC CGAAGTATTT TATGTTAAAA TTAAAAGATC TTTGTACTTA 
GCTGCGAAAC GGGCTACTCT TTTGGAACGA AGCCAAAAAG ACCCGATTCC CGGAAGCCAA 
GAGCAATTTG ACGACGAGAT AGAAAAGCTA CTGCATTCTT TGGACGAGGA TGATCGTAGT 
GTACCAGAAT CTCCAGTATT ACCAATTGAT AAAGATAAGG AAAGTAAGAA AGAAAAAGAA 
AAAGATTCTG TACAATCTCC AATTCATAGT GCTACAGCTC CAACTTCACC TGCTCCAGTT 
AGTGCTAGTC CAGTTACACA TTCAAAGTTT ATTTCATCGT ACAGCGATCA TAATGGCAAA 
TTAAAAAATT CGCCAGTAAA GGGAACGGTC ACGAGTCCGA ATCAAGGACA AACCAATTTA 
GATTTAAATT CAGACATTCT TGCTCGTCAT GAAGCTAATA AAATCATATC AGCAAAAAAA 
TTAATTGAAG AGGAAGAAAG AACGAATCTC CTGCCTAAGC AAGAAGACTC TTAA