LOC100880131 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100880131
Entrez Gene ID	100880131
Full Name	ras GTPase-activating-like protein IQGAP1
Synonyms	Iqgap1
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012296340.1	XP_012151730.1	ras GTPase-activating-like protein IQGAP1 isoform X2
XM_012296341.1	XP_012151731.1	ras GTPase-activating-like protein IQGAP1 isoform X3
XM_012296344.1	XP_012151734.1	ras GTPase-activating-like protein IQGAP1 isoform X6
XM_012296342.1	XP_012151732.1	ras GTPase-activating-like protein IQGAP1 isoform X4
XM_012296343.1	XP_012151733.1	ras GTPase-activating-like protein IQGAP1 isoform X5
XM_012296339.1	XP_012151729.1	ras GTPase-activating-like protein IQGAP1 isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC100880131 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100880131 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMe114322	XM_012296344.1 Latest version!	Megachile rotundata ras GTPase-activating-like protein IQGAP1 (LOC100880131), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OMe114317	XM_012296339.1 Latest version!	Megachile rotundata ras GTPase-activating-like protein IQGAP1 (LOC100880131), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OMe114318	XM_012296340.1 Latest version!	Megachile rotundata ras GTPase-activating-like protein IQGAP1 (LOC100880131), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OMe114319	XM_012296341.1 Latest version!	Megachile rotundata ras GTPase-activating-like protein IQGAP1 (LOC100880131), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OMe114320	XM_012296342.1 Latest version!	Megachile rotundata ras GTPase-activating-like protein IQGAP1 (LOC100880131), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OMe114321	XM_012296343.1 Latest version!	Megachile rotundata ras GTPase-activating-like protein IQGAP1 (LOC100880131), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100880131 ( XM_012296344.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012151734.1 Megachile rotundata ras GTPase-activating-like protein IQGAP1 (LOC100880131), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMe114322
Clone ID Related Accession (Same CDS sequence)	XM_012296344.1
Accession Version	XM_012296344.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4476bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	ras GTPase-activating-like protein IQGAP1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797713.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAATA AATATAATCA AAGAACTGAA CAAGCACCAC GTATTATGAA TGATATTGAT 
GATAAAATAA TTGATCATGA AAAGGACGAC GAAATAAATG CTGTTAGTAA AGAATTGAAG 
AAGTATGGAA TTCAAATGCC TTCGTTCCAA AAAATTGGCG GATTATTAGC GAACAGTATG 
GAGATCGATG CTGTCACGCT TCATGCTGCG GTAATAGCAA TTAACCAGGC CATCACAGAA 
AAAGATAATG TTAAAATGTT AATGGCGCTT CAGAATAAAG TAGTGCAAAT AAATAATATT 
AACCCTCTCT ACATTAAGGA ATATGGCGAT ACATTAACAG AGGCAAAGTA TTCTAAGCAA 
CAGGCTGCTC TTAATAGATC TCTTAACGAT AGTTATGTGC CAGATATTTA TGATGAATTG 
TTAACTCAAG CAGAAATTCA GGGACATATT AATTATGTTA ATGTCGAGTG CGCAGTGAAA 
AACATAAGTC GGTCAATTCA TCATAACAAT AACGAGTTCG TGAATGCACT GAGAGCATCT 
GCTTTGTGTT TAAAAAACAT TAAGCATGAA AATGCTGAAT TATATAAAGA ACAGTTAATG 
CAGTTACTAG AAAGTTCTGA ATGGAATAAT ATGTATTCGG AAAGCAATCA TCATTTGAAA 
CAATTGCTTC AGAAAGAAAT TGATAAATCA AATGAAACAG CGCTTAAATC TCGGAAACGT 
GAAGAAGGCG TTAAACTCTT AAACTCAACT TTACAAAATG GTTTGAAGAA TGAGTTCTCT 
GAGGCCTTAA AAAGTCCGAA ATTGGGATTG ATCGAACAAA TCGATGATTT TGCAATACCA 
TTATATTACG AAGAAATGAA AATCGATCGT ACGGAATCTC AGAACGACCT TTCATATAGT 
GATATTGTAG TGAGTATTCG TGTATTGTCA GTGATCGCAG CGATCAGTAA AGCTGTAGAT 
ACGGGAAATC CAGATTTAGT TTATCAGTCA TTAGAAAATC CAGACTGTCA TGTTTCTGGT 
TTGGATCATG AAAACAAAGT GAAGTATTAT AGAGCACTGT CAGCAGTTCG ATGTGAAAAA 
CAAGCAATTG CTGAGGAATG TCCTTTGCTA ACGTACATCG ATATCCAAGA GTGTATAGAT 
GCCGTAAATC AACAGCGTCA AAATGACGAC GATGTAATAC AAGTTTTACG TCAACTAAAT 
GCGGCTGTTG TAGAGAATGA TCGAGACGGA ATTATAATTG CTTTAAAAGA TACCGCTTTG 
AAGTTGCAGA AGTTTACTTC GCCAGAAGAT GCATCTCTCT ATTTGAAATT ATTTAAAAAA 
TGCCTTACTG AGAAACATTT TGATGGTTCT GAATTATGGT TAGAAGATGT AGAAACGATA 
ACAAAAATCG TTGCTGCGGA ATCTGAAAAT GTCCAAAATG CGTGTACGTT TCTGTCACAA 
GTAAATTTAG GTTTACAACA AAACGATATG TTATTCACAG TTGATTGCCT TCAGACGTTT 
GGTTTTAAAA TATCAGATAA ATACAAAACA GAGTGCTTTC AAAGTTTATG TAACTTACAA 
AAAATGAAGA GTAAAAAGTA CAATTGTGCT CTAGTTCGTT ATATCACTCC TAAAGGCAAC 
GAATCGTACT TGGATTTGAA AAAGTATAAT TATACTTGGG ATTGTCCAAA AAATATATCG 
GAAACATATT ATATCAACAT GGAAGATATC AAGGAAATAA TAAAAAGTAC TACCGCAAGA 
GAACACGAAG AGCATAAAAT CAGCAAACGG ATAGTTCGAT TGCAAGCTTG CATACGAGGA 
TATTTATTAC GAAAAAAAAT TTCTGTTAGA TTTGCATATT TTTATGACAA CGTAGATAAA 
GTTATTAAAA TACAAGCCTG GTGGCGCGGT ATATTACAAC GTAAACGATA CGCCAAATTA 
TTAACAGAAA GAAGAAATTA TAGTAGTGAA AAATTTTCCA GTAACTGTTT AAGAATTAAA 
AATAAGTATG CAAATGTATT AGATTACTAT AAACAACATG AGGAAAAGAT AATAAAGATA 
CAAGCTTTGT GGCGTGGCCG TGCAGAAAGA CGAGCGTTTC ACTCATTGTT GTACATGGAA 
AAACCACCAT TTCCTGTTGT CCGACATTTT TCTGCACTTC TAAATTTTAA TGCAGAAGAT 
TATGACAGAG ATTTACAATT ACAACAATTA AAACACGAAG TGGTACAAAA CATACGGCAT 
AATCAAACTT TATCGCAACA ATTGGATAGT ATGGACATAA AAATTGGTTT GCTTATTCAA 
AATAGGATCA CGCTACAAGA TGTTGTAGCA CATGGGAAGA GTTTAGAAAC ACTTGCGAAG 
CAGAAAAATT CAACTAGAGA TCAGAAGAAT GTTTTATCAG ATAGTACTAT TTCGCATAAA 
GGTTTGAAAT CTTTAACAAA AGAAGGTCGA AAAATGTTAG AGGGGTATCA GCATCTATTC 
TATGCGTTAC AAACTAATCC AACATATTTA TCGAAGATTT TATTTCTTTT GCCGCAAAGC 
AAAACGAATA AGTTTCTTCA AAATGTTATT TTAACGTTAT ATAATTTCGG ATCAAATATA 
AGAGAAGAGT ATTTATTATT GAAACTTTTT GGCAGTGCAT TACAAGAAGA AATTAGGTGT 
AAATTTCAAA AACCGTCTGA AGTCGTAACT GGAAATCCAC TCGTCTTAAA AATGGTAGTG 
AATTACGCAC GACAATTACA TGGTCAAAGA GCTTTACGAC AAATTGTTGG GCCAATCATT 
GAAAAGATAT TAGCCGACCG AACATTGAAT ATAGAAACGA ATCCCGTTGA TATTTATAAG 
TGTTGGAGGA ATCAATTAGA AATGGAGACT GGAGAAACAC TGAATCTTCC ATATACGGTA 
ACGCAACAAC AAGCTTTAAA TTACGAACAG GTACGAATCA GATTAAATAA AGGGATTCAA 
TTGTTGCAAA GTACCGTCTT AGAATTCTTA ACTAAAATAA CAGAATCTCG AGATCTAATA 
CCATACGGAA TGTTATACAT GGCGAAAATT TTAAACGATT CTTTGACTGA AAAATTTCCA 
AACGCTCCGG AAAAAGATAT TCTCAAAGTA GTTGGAAACT TGATTTATTA TCATTTCATT 
AACGCAGCTA TCGTAGCTCC AGATGCTTTC GACATAATTA CCTTACCGAT TGATAGATCA 
TTGTCAAACG ATCAAAGGAG AAATTTAGCC AGCATTGCAA AAATACTACA ATTTGCTGCT 
TCTAAAAAGG GGTTTGGAGA GGAAGCCACA CATTTAGTGT GTCTGAACTC TTTTATAATC 
GAATGCCACG AAAAATTTAA AAAATTCTTT CGTTTTTGTT GCCAAATTGA AGATTTGGAA 
GAACATTTCT GTATTCATGA GTACACCGAG GCCACGCTTA TTCAGAAGCC TGAGATCTAT 
ATTTCCTTAC AAGAAATTTG CGATACTCAC TGCCTCATGT TAGAGTATCA AGACCAAATA 
GCGCCAGATC CAATGGATCC CTTGCATGAT TTGTTAGATG ATTTAGGACC TGCACCTACC 
GTCGCATCTC TGCTAGGAAT CTCCGATACG ATGTGTGAGG CAAATTTAAT ACGGTTTGGA 
AAAACGGAGG TATGTCTGGT ACTTACCAAC AAATTTCAAG TACCCGAAGA TGAAGATGCT 
AGTTTAAACA AACTTTTTAT AAAAACTAAA GAGCTATTGG TTTCTGTACT CCAATTCTTG 
AAAGGACAAA CACTAGTAGA GGCTTTGGAA ACCACTTGTT CTCCTGTTCA AGAGAAACTC 
TATGATGTTA AGTGTTCTAG TTTATCGCCC ACTCTAAGTA TCATTCACAA AAGTTCGTCT 
TTAAATGATT GTAAACTTCA ACTTCGTGCT TACCTTAATA AACTTGAACT TGGAGGATGG 
GTGAGTCGAT CGGATGGATA TCAGACTATT ATAACAGCCG TATCTAAAGA TCTTTGTAAC 
AAAGGAAAGT ACCGAATCAT CCGAAATAAA GAATTACAAA CTTTACGCAT AACTAAACAA 
AGACTGGAAG AAAAGTGCAA ATATTACCAG GAACAGGTAG AATATTATAA CGAATATATA 
CAACGTTGCT TGGAGAACTT GCATACGGGA AAAGGATCTT TACGAGCATT TAAAGCCATT 
CAAAAGAATA ATCATGGCAA GTTACGGTCT AAAATGACAT TGAAATATTC AGCGGCCAAG 
CTACAAGAGA AAGGAATATT ATTAGAAGTG GATGGACTTC CACAATCACA ATTTAAGAAC 
GTAATTTTCG AGATCAGCCC AACAGAGCAT ACCGGATTAT TTAATGTGCG GTGTAAATTT 
ATGGGAGTAG AAATGGAGAA AGTTGATATT GATATACAAA AGCTGCTTGA ACTACAATTT 
GAGGGTTCAC CGATTATGGA CATGTTCGGT AAAGCGAAAG TTAACGTGAA CTTATTACTA 
TATTTACTAA ATCGAAAATT TTATGGTAAA ACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012151734.1
CDS	34..4509
Translation	MNNKYNQRTEQAPRIMNDIDDKIIDHEKDDEINAVSKELKKYGIQMPSFQKIGGLLANSMEIDAVTLHAAVIAINQAITEKDNVKMLMALQNKVVQINNINPLYIKEYGDTLTEAKYSKQQAALNRSLNDSYVPDIYDELLTQAEIQGHINYVNVECAVKNISRSIHHNNNEFVNALRASALCLKNIKHENAELYKEQLMQLLESSEWNNMYSESNHHLKQLLQKEIDKSNETALKSRKREEGVKLLNSTLQNGLKNEFSEALKSPKLGLIEQIDDFAIPLYYEEMKIDRTESQNDLSYSDIVVSIRVLSVIAAISKAVDTGNPDLVYQSLENPDCHVSGLDHENKVKYYRALSAVRCEKQAIAEECPLLTYIDIQECIDAVNQQRQNDDDVIQVLRQLNAAVVENDRDGIIIALKDTALKLQKFTSPEDASLYLKLFKKCLTEKHFDGSELWLEDVETITKIVAAESENVQNACTFLSQVNLGLQQNDMLFTVDCLQTFGFKISDKYKTECFQSLCNLQKMKSKKYNCALVRYITPKGNESYLDLKKYNYTWDCPKNISETYYINMEDIKEIIKSTTAREHEEHKISKRIVRLQACIRGYLLRKKISVRFAYFYDNVDKVIKIQAWWRGILQRKRYAKLLTERRNYSSEKFSSNCLRIKNKYANVLDYYKQHEEKIIKIQALWRGRAERRAFHSLLYMEKPPFPVVRHFSALLNFNAEDYDRDLQLQQLKHEVVQNIRHNQTLSQQLDSMDIKIGLLIQNRITLQDVVAHGKSLETLAKQKNSTRDQKNVLSDSTISHKGLKSLTKEGRKMLEGYQHLFYALQTNPTYLSKILFLLPQSKTNKFLQNVILTLYNFGSNIREEYLLLKLFGSALQEEIRCKFQKPSEVVTGNPLVLKMVVNYARQLHGQRALRQIVGPIIEKILADRTLNIETNPVDIYKCWRNQLEMETGETLNLPYTVTQQQALNYEQVRIRLNKGIQLLQSTVLEFLTKITESRDLIPYGMLYMAKILNDSLTEKFPNAPEKDILKVVGNLIYYHFINAAIVAPDAFDIITLPIDRSLSNDQRRNLASIAKILQFAASKKGFGEEATHLVCLNSFIIECHEKFKKFFRFCCQIEDLEEHFCIHEYTEATLIQKPEIYISLQEICDTHCLMLEYQDQIAPDPMDPLHDLLDDLGPAPTVASLLGISDTMCEANLIRFGKTEVCLVLTNKFQVPEDEDASLNKLFIKTKELLVSVLQFLKGQTLVEALETTCSPVQEKLYDVKCSSLSPTLSIIHKSSSLNDCKLQLRAYLNKLELGGWVSRSDGYQTIITAVSKDLCNKGKYRIIRNKELQTLRITKQRLEEKCKYYQEQVEYYNEYIQRCLENLHTGKGSLRAFKAIQKNNHGKLRSKMTLKYSAAKLQEKGILLEVDGLPQSQFKNVIFEISPTEHTGLFNVRCKFMGVEMEKVDIDIQKLLELQFEGSPIMDMFGKAKVNVNLLLYLLNRKFYGKT

Target ORF information:

RefSeq Version	XM_012296344.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata ras GTPase-activating-like protein IQGAP1 (LOC100880131), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012296344.1

ORF Insert Sequence:

ATGAATAATA AATATAATCA AAGAACTGAA CAAGCACCAC GTATTATGAA TGATATTGAT 
GATAAAATAA TTGATCATGA AAAGGACGAC GAAATAAATG CTGTTAGTAA AGAATTGAAG 
AAGTATGGAA TTCAAATGCC TTCGTTCCAA AAAATTGGCG GATTATTAGC GAACAGTATG 
GAGATCGATG CTGTCACGCT TCATGCTGCG GTAATAGCAA TTAACCAGGC CATCACAGAA 
AAAGATAATG TTAAAATGTT AATGGCGCTT CAGAATAAAG TAGTGCAAAT AAATAATATT 
AACCCTCTCT ACATTAAGGA ATATGGCGAT ACATTAACAG AGGCAAAGTA TTCTAAGCAA 
CAGGCTGCTC TTAATAGATC TCTTAACGAT AGTTATGTGC CAGATATTTA TGATGAATTG 
TTAACTCAAG CAGAAATTCA GGGACATATT AATTATGTTA ATGTCGAGTG CGCAGTGAAA 
AACATAAGTC GGTCAATTCA TCATAACAAT AACGAGTTCG TGAATGCACT GAGAGCATCT 
GCTTTGTGTT TAAAAAACAT TAAGCATGAA AATGCTGAAT TATATAAAGA ACAGTTAATG 
CAGTTACTAG AAAGTTCTGA ATGGAATAAT ATGTATTCGG AAAGCAATCA TCATTTGAAA 
CAATTGCTTC AGAAAGAAAT TGATAAATCA AATGAAACAG CGCTTAAATC TCGGAAACGT 
GAAGAAGGCG TTAAACTCTT AAACTCAACT TTACAAAATG GTTTGAAGAA TGAGTTCTCT 
GAGGCCTTAA AAAGTCCGAA ATTGGGATTG ATCGAACAAA TCGATGATTT TGCAATACCA 
TTATATTACG AAGAAATGAA AATCGATCGT ACGGAATCTC AGAACGACCT TTCATATAGT 
GATATTGTAG TGAGTATTCG TGTATTGTCA GTGATCGCAG CGATCAGTAA AGCTGTAGAT 
ACGGGAAATC CAGATTTAGT TTATCAGTCA TTAGAAAATC CAGACTGTCA TGTTTCTGGT 
TTGGATCATG AAAACAAAGT GAAGTATTAT AGAGCACTGT CAGCAGTTCG ATGTGAAAAA 
CAAGCAATTG CTGAGGAATG TCCTTTGCTA ACGTACATCG ATATCCAAGA GTGTATAGAT 
GCCGTAAATC AACAGCGTCA AAATGACGAC GATGTAATAC AAGTTTTACG TCAACTAAAT 
GCGGCTGTTG TAGAGAATGA TCGAGACGGA ATTATAATTG CTTTAAAAGA TACCGCTTTG 
AAGTTGCAGA AGTTTACTTC GCCAGAAGAT GCATCTCTCT ATTTGAAATT ATTTAAAAAA 
TGCCTTACTG AGAAACATTT TGATGGTTCT GAATTATGGT TAGAAGATGT AGAAACGATA 
ACAAAAATCG TTGCTGCGGA ATCTGAAAAT GTCCAAAATG CGTGTACGTT TCTGTCACAA 
GTAAATTTAG GTTTACAACA AAACGATATG TTATTCACAG TTGATTGCCT TCAGACGTTT 
GGTTTTAAAA TATCAGATAA ATACAAAACA GAGTGCTTTC AAAGTTTATG TAACTTACAA 
AAAATGAAGA GTAAAAAGTA CAATTGTGCT CTAGTTCGTT ATATCACTCC TAAAGGCAAC 
GAATCGTACT TGGATTTGAA AAAGTATAAT TATACTTGGG ATTGTCCAAA AAATATATCG 
GAAACATATT ATATCAACAT GGAAGATATC AAGGAAATAA TAAAAAGTAC TACCGCAAGA 
GAACACGAAG AGCATAAAAT CAGCAAACGG ATAGTTCGAT TGCAAGCTTG CATACGAGGA 
TATTTATTAC GAAAAAAAAT TTCTGTTAGA TTTGCATATT TTTATGACAA CGTAGATAAA 
GTTATTAAAA TACAAGCCTG GTGGCGCGGT ATATTACAAC GTAAACGATA CGCCAAATTA 
TTAACAGAAA GAAGAAATTA TAGTAGTGAA AAATTTTCCA GTAACTGTTT AAGAATTAAA 
AATAAGTATG CAAATGTATT AGATTACTAT AAACAACATG AGGAAAAGAT AATAAAGATA 
CAAGCTTTGT GGCGTGGCCG TGCAGAAAGA CGAGCGTTTC ACTCATTGTT GTACATGGAA 
AAACCACCAT TTCCTGTTGT CCGACATTTT TCTGCACTTC TAAATTTTAA TGCAGAAGAT 
TATGACAGAG ATTTACAATT ACAACAATTA AAACACGAAG TGGTACAAAA CATACGGCAT 
AATCAAACTT TATCGCAACA ATTGGATAGT ATGGACATAA AAATTGGTTT GCTTATTCAA 
AATAGGATCA CGCTACAAGA TGTTGTAGCA CATGGGAAGA GTTTAGAAAC ACTTGCGAAG 
CAGAAAAATT CAACTAGAGA TCAGAAGAAT GTTTTATCAG ATAGTACTAT TTCGCATAAA 
GGTTTGAAAT CTTTAACAAA AGAAGGTCGA AAAATGTTAG AGGGGTATCA GCATCTATTC 
TATGCGTTAC AAACTAATCC AACATATTTA TCGAAGATTT TATTTCTTTT GCCGCAAAGC 
AAAACGAATA AGTTTCTTCA AAATGTTATT TTAACGTTAT ATAATTTCGG ATCAAATATA 
AGAGAAGAGT ATTTATTATT GAAACTTTTT GGCAGTGCAT TACAAGAAGA AATTAGGTGT 
AAATTTCAAA AACCGTCTGA AGTCGTAACT GGAAATCCAC TCGTCTTAAA AATGGTAGTG 
AATTACGCAC GACAATTACA TGGTCAAAGA GCTTTACGAC AAATTGTTGG GCCAATCATT 
GAAAAGATAT TAGCCGACCG AACATTGAAT ATAGAAACGA ATCCCGTTGA TATTTATAAG 
TGTTGGAGGA ATCAATTAGA AATGGAGACT GGAGAAACAC TGAATCTTCC ATATACGGTA 
ACGCAACAAC AAGCTTTAAA TTACGAACAG GTACGAATCA GATTAAATAA AGGGATTCAA 
TTGTTGCAAA GTACCGTCTT AGAATTCTTA ACTAAAATAA CAGAATCTCG AGATCTAATA 
CCATACGGAA TGTTATACAT GGCGAAAATT TTAAACGATT CTTTGACTGA AAAATTTCCA 
AACGCTCCGG AAAAAGATAT TCTCAAAGTA GTTGGAAACT TGATTTATTA TCATTTCATT 
AACGCAGCTA TCGTAGCTCC AGATGCTTTC GACATAATTA CCTTACCGAT TGATAGATCA 
TTGTCAAACG ATCAAAGGAG AAATTTAGCC AGCATTGCAA AAATACTACA ATTTGCTGCT 
TCTAAAAAGG GGTTTGGAGA GGAAGCCACA CATTTAGTGT GTCTGAACTC TTTTATAATC 
GAATGCCACG AAAAATTTAA AAAATTCTTT CGTTTTTGTT GCCAAATTGA AGATTTGGAA 
GAACATTTCT GTATTCATGA GTACACCGAG GCCACGCTTA TTCAGAAGCC TGAGATCTAT 
ATTTCCTTAC AAGAAATTTG CGATACTCAC TGCCTCATGT TAGAGTATCA AGACCAAATA 
GCGCCAGATC CAATGGATCC CTTGCATGAT TTGTTAGATG ATTTAGGACC TGCACCTACC 
GTCGCATCTC TGCTAGGAAT CTCCGATACG ATGTGTGAGG CAAATTTAAT ACGGTTTGGA 
AAAACGGAGG TATGTCTGGT ACTTACCAAC AAATTTCAAG TACCCGAAGA TGAAGATGCT 
AGTTTAAACA AACTTTTTAT AAAAACTAAA GAGCTATTGG TTTCTGTACT CCAATTCTTG 
AAAGGACAAA CACTAGTAGA GGCTTTGGAA ACCACTTGTT CTCCTGTTCA AGAGAAACTC 
TATGATGTTA AGTGTTCTAG TTTATCGCCC ACTCTAAGTA TCATTCACAA AAGTTCGTCT 
TTAAATGATT GTAAACTTCA ACTTCGTGCT TACCTTAATA AACTTGAACT TGGAGGATGG 
GTGAGTCGAT CGGATGGATA TCAGACTATT ATAACAGCCG TATCTAAAGA TCTTTGTAAC 
AAAGGAAAGT ACCGAATCAT CCGAAATAAA GAATTACAAA CTTTACGCAT AACTAAACAA 
AGACTGGAAG AAAAGTGCAA ATATTACCAG GAACAGGTAG AATATTATAA CGAATATATA 
CAACGTTGCT TGGAGAACTT GCATACGGGA AAAGGATCTT TACGAGCATT TAAAGCCATT 
CAAAAGAATA ATCATGGCAA GTTACGGTCT AAAATGACAT TGAAATATTC AGCGGCCAAG 
CTACAAGAGA AAGGAATATT ATTAGAAGTG GATGGACTTC CACAATCACA ATTTAAGAAC 
GTAATTTTCG AGATCAGCCC AACAGAGCAT ACCGGATTAT TTAATGTGCG GTGTAAATTT 
ATGGGAGTAG AAATGGAGAA AGTTGATATT GATATACAAA AGCTGCTTGA ACTACAATTT 
GAGGGTTCAC CGATTATGGA CATGTTCGGT AAAGCGAAAG TTAACGTGAA CTTATTACTA 
TATTTACTAA ATCGAAAATT TTATGGTAAA ACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100880131 ( XM_012296339.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012151729.1 Megachile rotundata ras GTPase-activating-like protein IQGAP1 (LOC100880131), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMe114317
Clone ID Related Accession (Same CDS sequence)	XM_012296339.1
Accession Version	XM_012296339.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	ras GTPase-activating-like protein IQGAP1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797713.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAATA AATATAATCA AAGAACTGAA CAAGCACCAC GTATTATGAA TGATATTGAT 
GATAAAATAA TTGATCATGA AAAGGAAGTT GAGACTCGGA AATCAGCAGA AGAGATGGAC 
GAACAACGTC AAAAAACTCT TGCTTACGAG TACCTTTGTC ACTTAGAGGA AGCTAAAAAA 
TGGCTGGAAG CCTGTCTGAG AGAAACTCTC CCACAAACTA TTGAATTAGA AGAAAACTTA 
AGGAACGGGG TATATTTAGC AAAACTTGCA CATTTTATGG CACCTGATTC TTTACCCTTT 
AATAAAATTT ATGACCCTGA ACAGAAGCGT TATACTGCGG TTGGTTTACA GTTTCGTCAT 
ACAGATAATA TTAATTATTT TTTAAAATCC TTAAAAACTG TGCAATTACC ATTGACTTTC 
CAACCAGAAA CTACAGACAT CTATGATAAA AAAAATATGC CACGCGTTAT ATATTGCATT 
CATGCTCTTA GTACTCATTT ATTCAAACTT GGTAGAGCTC CACAGATTCA GGATTTGTAT 
GGAAAAGTGA ACTTCACTGA CGACGAAATA AATGCTGTTA GTAAAGAATT GAAGAAGTAT 
GGAATTCAAA TGCCTTCGTT CCAAAAAATT GGCGGATTAT TAGCGAACAG TATGGAGATC 
GATGCTGTCA CGCTTCATGC TGCGGTAATA GCAATTAACC AGGCCATCAC AGAAAAAGAT 
AATGTTAAAA TGTTAATGGC GCTTCAGAAT AAAGTAGTGC AAATAAATAA TATTAACCCT 
CTCTACATTA AGGAATATGG CGATACATTA ACAGAGGCAA AGTATTCTAA GCAACAGGCT 
GCTCTTAATA GATCTCTTAA CGATAGTTAT GTGCCAGATA TTTATGATGA ATTGTTAACT 
CAAGCAGAAA TTCAGGGACA TATTAATTAT GTTAATGTCG AGTGCGCAGT GAAAAACATA 
AGTCGGTCAA TTCATCATAA CAATAACGAG TTCGTGAATG CACTGAGAGC ATCTGCTTTG 
TGTTTAAAAA ACATTAAGCA TGAAAATGCT GAATTATATA AAGAACAGTT AATGCAGTTA 
CTAGAAAGTT CTGAATGGAA TAATATGTAT TCGGAAAGCA ATCATCATTT GAAACAATTG 
CTTCAGAAAG AAATTGATAA ATCAAATGAA ACAGCGCTTA AATCTCGGAA ACGTGAAGAA 
GGCGTTAAAC TCTTAAACTC AACTTTACAA AATGGTTTGA AGAATGAGTT CTCTGAGGCC 
TTAAAAAGTC CGAAATTGGG ATTGATCGAA CAAATCGATG ATTTTGCAAT ACCATTATAT 
TACGAAGAAA TGAAAATCGA TCGTACGGAA TCTCAGAACG ACCTTTCATA TAGTGATATT 
GTAGTGAGTA TTCGTGTATT GTCAGTGATC GCAGCGATCA GTAAAGCTGT AGATACGGGA 
AATCCAGATT TAGTTTATCA GTCATTAGAA AATCCAGACT GTCATGTTTC TGGTTTGGAT 
CATGAAAACA AAGTGAAGTA TTATAGAGCA CTGTCAGCAG TTCGATGTGA AAAACAAGCA 
ATTGCTGAGG AATGTCCTTT GCTAACGTAC ATCGATATCC AAGAGTGTAT AGATGCCGTA 
AATCAACAGC GTCAAAATGA CGACGATGTA ATACAAGTTT TACGTCAACT AAATGCGGCT 
GTTGTAGAGA ATGATCGAGA CGGAATTATA ATTGCTTTAA AAGATACCGC TTTGAAGTTG 
CAGAAGTTTA CTTCGCCAGA AGATGCATCT CTCTATTTGA AATTATTTAA AAAATGCCTT 
ACTGAGAAAC ATTTTGATGG TTCTGAATTA TGGTTAGAAG ATGTAGAAAC GATAACAAAA 
ATCGTTGCTG CGGAATCTGA AAATGTCCAA AATGCGTGTA CGTTTCTGTC ACAAGTAAAT 
TTAGGTTTAC AACAAAACGA TATGTTATTC ACAGTTGATT GCCTTCAGAC GTTTGGTTTT 
AAAATATCAG ATAAATACAA AACAGAGTGC TTTCAAAGTT TATGTAACTT ACAAAAAATG 
AAGAGTAAAA AGTACAATTG TGCTCTAGTT CGTTATATCA CTCCTAAAGG CAACGAATCG 
TACTTGGATT TGAAAAAGTA TAATTATACT TGGGATTGTC CAAAAAATAT ATCGGAAACA 
TATTATATCA ACATGGAAGA TATCAAGGAA ATAATAAAAA GTACTACCGC AAGAGAACAC 
GAAGAGCATA AAATCAGCAA ACGGATAGTT CGATTGCAAG CTTGCATACG AGGATATTTA 
TTACGAAAAA AAATTTCTGT TAGATTTGCA TATTTTTATG ACAACGTAGA TAAAGTTATT 
AAAATACAAG CCTGGTGGCG CGGTATATTA CAACGTAAAC GATACGCCAA ATTATTAACA 
GAAAGAAGAA ATTATAGTAG TGAAAAATTT TCCAGTAACT GTTTAAGAAT TAAAAATAAG 
TATGCAAATG TATTAGATTA CTATAAACAA CATGAGGAAA AGATAATAAA GATACAAGCT 
TTGTGGCGTG GCCGTGCAGA AAGACGAGCG TTTCACTCAT TGTTGTACAT GGAAAAACCA 
CCATTTCCTG TTGTCCGACA TTTTTCTGCA CTTCTAAATT TTAATGCAGA AGATTATGAC 
AGAGATTTAC AATTACAACA ATTAAAACAC GAAGTGGTAC AAAACATACG GCATAATCAA 
ACTTTATCGC AACAATTGGA TAGTATGGAC ATAAAAATTG GTTTGCTTAT TCAAAATAGG 
ATCACGCTAC AAGATGTTGT AGCACATGGG AAGAGTTTAG AAACACTTGC GAAGCAGAAA 
AATTCAACTA GAGATCAGAA GAATGTTTTA TCAGATAGTA CTATTTCGCA TAAAGGTTTG 
AAATCTTTAA CAAAAGAAGG TCGAAAAATG TTAGAGGGGT ATCAGCATCT ATTCTATGCG 
TTACAAACTA ATCCAACATA TTTATCGAAG ATTTTATTTC TTTTGCCGCA AAGCAAAACG 
AATAAGTTTC TTCAAAATGT TATTTTAACG TTATATAATT TCGGATCAAA TATAAGAGAA 
GAGTATTTAT TATTGAAACT TTTTGGCAGT GCATTACAAG AAGAAATTAG GTGTAAATTT 
CAAAAACCGT CTGAAGTCGT AACTGGAAAT CCACTCGTCT TAAAAATGGT AGTGAATTAC 
GCACGACAAT TACATGGTCA AAGAGCTTTA CGACAAATTG TTGGGCCAAT CATTGAAAAG 
ATATTAGCCG ACCGAACATT GAATATAGAA ACGAATCCCG TTGATATTTA TAAGTGTTGG 
AGGAATCAAT TAGAAATGGA GACTGGAGAA ACACTGAATC TTCCATATAC GGTAACGCAA 
CAACAAGCTT TAAATTACGA ACAGGTACGA ATCAGATTAA ATAAAGGGAT TCAATTGTTG 
CAAAGTACCG TCTTAGAATT CTTAACTAAA ATAACAGAAT CTCGAGATCT AATACCATAC 
GGAATGTTAT ACATGGCGAA AATTTTAAAC GATTCTTTGA CTGAAAAATT TCCAAACGCT 
CCGGAAAAAG ATATTCTCAA AGTAGTTGGA AACTTGATTT ATTATCATTT CATTAACGCA 
GCTATCGTAG CTCCAGATGC TTTCGACATA ATTACCTTAC CGATTGATAG ATCATTGTCA 
AACGATCAAA GGAGAAATTT AGCCAGCATT GCAAAAATAC TACAATTTGC TGCTTCTAAA 
AAGGGGTTTG GAGAGGAAGC CACACATTTA GTGTGTCTGA ACTCTTTTAT AATCGAATGC 
CACGAAAAAT TTAAAAAATT CTTTCGTTTT TGTTGCCAAA TTGAAGATTT GGAAGAACAT 
TTCTGTATTC ATGAGTACAC CGAGGCCACG CTTATTCAGA AGCCTGAGAT CTATATTTCC 
TTACAAGAAA TTTGCGATAC TCACTGCCTC ATGTTAGAGT ATCAAGACCA AATAGCGCCA 
GATCCAATGG ATCCCTTGCA TGATTTGTTA GATGATTTAG GACCTGCACC TACCGTCGCA 
TCTCTGCTAG GAATCTCCGA TACGATGTGT GAGGCAAATT TAATACGGTT TGGAAAAACG 
GAGGTATGTC TGGTACTTAC CAACAAATTT CAAGTACCCG AAGATGAAGA TGCTAGTTTA 
AACAAACTTT TTATAAAAAC TAAAGAGCTA TTGGTTTCTG TACTCCAATT CTTGAAAGGA 
CAAACACTAG TAGAGGCTTT GGAAACCACT TGTTCTCCTG TTCAAGAGAA ACTCTATGAT 
GTTAAGTGTT CTAGTTTATC GCCCACTCTA AGTATCATTC ACAAAAGTTC GTCTTTAAAT 
GATTGTAAAC TTCAACTTCG TGCTTACCTT AATAAACTTG AACTTGGAGG ATGGGTGAGT 
CGATCGGATG GATATCAGAC TATTATAACA GCCGTATCTA AAGATCTTTG TAACAAAGGA 
AAGTACCGAA TCATCCGAAA TAAAGAATTA CAAACTTTAC GCATAACTAA ACAAAGACTG 
GAAGAAAAGT GCAAATATTA CCAGGAACAG GTAGAATATT ATAACGAATA TATACAACGT 
TGCTTGGAGA ACTTGCATAC GGGAAAAGGA TCTTTACGAG CATTTAAAGC CATTCAAAAG 
AATAATCATG GCAAGTTACG GTCTAAAATG ACATTGAAAT ATTCAGCGGC CAAGCTACAA 
GAGAAAGGAA TATTATTAGA AGTGGATGGA CTTCCACAAT CACAATTTAA GAACGTAATT 
TTCGAGATCA GCCCAACAGA GCATACCGGA TTATTTAATG TGCGGTGTAA ATTTATGGGA 
GTAGAAATGG AGAAAGTTGA TATTGATATA CAAAAGCTGC TTGAACTACA ATTTGAGGGT 
TCACCGATTA TGGACATGTT CGGTAAAGCG AAAGTTAACG TGAACTTATT ACTATATTTA 
CTAAATCGAA AATTTTATGG TAAAACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012151729.1
CDS	34..4983
Translation	MNNKYNQRTEQAPRIMNDIDDKIIDHEKEVETRKSAEEMDEQRQKTLAYEYLCHLEEAKKWLEACLRETLPQTIELEENLRNGVYLAKLAHFMAPDSLPFNKIYDPEQKRYTAVGLQFRHTDNINYFLKSLKTVQLPLTFQPETTDIYDKKNMPRVIYCIHALSTHLFKLGRAPQIQDLYGKVNFTDDEINAVSKELKKYGIQMPSFQKIGGLLANSMEIDAVTLHAAVIAINQAITEKDNVKMLMALQNKVVQINNINPLYIKEYGDTLTEAKYSKQQAALNRSLNDSYVPDIYDELLTQAEIQGHINYVNVECAVKNISRSIHHNNNEFVNALRASALCLKNIKHENAELYKEQLMQLLESSEWNNMYSESNHHLKQLLQKEIDKSNETALKSRKREEGVKLLNSTLQNGLKNEFSEALKSPKLGLIEQIDDFAIPLYYEEMKIDRTESQNDLSYSDIVVSIRVLSVIAAISKAVDTGNPDLVYQSLENPDCHVSGLDHENKVKYYRALSAVRCEKQAIAEECPLLTYIDIQECIDAVNQQRQNDDDVIQVLRQLNAAVVENDRDGIIIALKDTALKLQKFTSPEDASLYLKLFKKCLTEKHFDGSELWLEDVETITKIVAAESENVQNACTFLSQVNLGLQQNDMLFTVDCLQTFGFKISDKYKTECFQSLCNLQKMKSKKYNCALVRYITPKGNESYLDLKKYNYTWDCPKNISETYYINMEDIKEIIKSTTAREHEEHKISKRIVRLQACIRGYLLRKKISVRFAYFYDNVDKVIKIQAWWRGILQRKRYAKLLTERRNYSSEKFSSNCLRIKNKYANVLDYYKQHEEKIIKIQALWRGRAERRAFHSLLYMEKPPFPVVRHFSALLNFNAEDYDRDLQLQQLKHEVVQNIRHNQTLSQQLDSMDIKIGLLIQNRITLQDVVAHGKSLETLAKQKNSTRDQKNVLSDSTISHKGLKSLTKEGRKMLEGYQHLFYALQTNPTYLSKILFLLPQSKTNKFLQNVILTLYNFGSNIREEYLLLKLFGSALQEEIRCKFQKPSEVVTGNPLVLKMVVNYARQLHGQRALRQIVGPIIEKILADRTLNIETNPVDIYKCWRNQLEMETGETLNLPYTVTQQQALNYEQVRIRLNKGIQLLQSTVLEFLTKITESRDLIPYGMLYMAKILNDSLTEKFPNAPEKDILKVVGNLIYYHFINAAIVAPDAFDIITLPIDRSLSNDQRRNLASIAKILQFAASKKGFGEEATHLVCLNSFIIECHEKFKKFFRFCCQIEDLEEHFCIHEYTEATLIQKPEIYISLQEICDTHCLMLEYQDQIAPDPMDPLHDLLDDLGPAPTVASLLGISDTMCEANLIRFGKTEVCLVLTNKFQVPEDEDASLNKLFIKTKELLVSVLQFLKGQTLVEALETTCSPVQEKLYDVKCSSLSPTLSIIHKSSSLNDCKLQLRAYLNKLELGGWVSRSDGYQTIITAVSKDLCNKGKYRIIRNKELQTLRITKQRLEEKCKYYQEQVEYYNEYIQRCLENLHTGKGSLRAFKAIQKNNHGKLRSKMTLKYSAAKLQEKGILLEVDGLPQSQFKNVIFEISPTEHTGLFNVRCKFMGVEMEKVDIDIQKLLELQFEGSPIMDMFGKAKVNVNLLLYLLNRKFYGKT

Target ORF information:

RefSeq Version	XM_012296339.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata ras GTPase-activating-like protein IQGAP1 (LOC100880131), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012296339.1

ORF Insert Sequence:

ATGAATAATA AATATAATCA AAGAACTGAA CAAGCACCAC GTATTATGAA TGATATTGAT 
GATAAAATAA TTGATCATGA AAAGGAAGTT GAGACTCGGA AATCAGCAGA AGAGATGGAC 
GAACAACGTC AAAAAACTCT TGCTTACGAG TACCTTTGTC ACTTAGAGGA AGCTAAAAAA 
TGGCTGGAAG CCTGTCTGAG AGAAACTCTC CCACAAACTA TTGAATTAGA AGAAAACTTA 
AGGAACGGGG TATATTTAGC AAAACTTGCA CATTTTATGG CACCTGATTC TTTACCCTTT 
AATAAAATTT ATGACCCTGA ACAGAAGCGT TATACTGCGG TTGGTTTACA GTTTCGTCAT 
ACAGATAATA TTAATTATTT TTTAAAATCC TTAAAAACTG TGCAATTACC ATTGACTTTC 
CAACCAGAAA CTACAGACAT CTATGATAAA AAAAATATGC CACGCGTTAT ATATTGCATT 
CATGCTCTTA GTACTCATTT ATTCAAACTT GGTAGAGCTC CACAGATTCA GGATTTGTAT 
GGAAAAGTGA ACTTCACTGA CGACGAAATA AATGCTGTTA GTAAAGAATT GAAGAAGTAT 
GGAATTCAAA TGCCTTCGTT CCAAAAAATT GGCGGATTAT TAGCGAACAG TATGGAGATC 
GATGCTGTCA CGCTTCATGC TGCGGTAATA GCAATTAACC AGGCCATCAC AGAAAAAGAT 
AATGTTAAAA TGTTAATGGC GCTTCAGAAT AAAGTAGTGC AAATAAATAA TATTAACCCT 
CTCTACATTA AGGAATATGG CGATACATTA ACAGAGGCAA AGTATTCTAA GCAACAGGCT 
GCTCTTAATA GATCTCTTAA CGATAGTTAT GTGCCAGATA TTTATGATGA ATTGTTAACT 
CAAGCAGAAA TTCAGGGACA TATTAATTAT GTTAATGTCG AGTGCGCAGT GAAAAACATA 
AGTCGGTCAA TTCATCATAA CAATAACGAG TTCGTGAATG CACTGAGAGC ATCTGCTTTG 
TGTTTAAAAA ACATTAAGCA TGAAAATGCT GAATTATATA AAGAACAGTT AATGCAGTTA 
CTAGAAAGTT CTGAATGGAA TAATATGTAT TCGGAAAGCA ATCATCATTT GAAACAATTG 
CTTCAGAAAG AAATTGATAA ATCAAATGAA ACAGCGCTTA AATCTCGGAA ACGTGAAGAA 
GGCGTTAAAC TCTTAAACTC AACTTTACAA AATGGTTTGA AGAATGAGTT CTCTGAGGCC 
TTAAAAAGTC CGAAATTGGG ATTGATCGAA CAAATCGATG ATTTTGCAAT ACCATTATAT 
TACGAAGAAA TGAAAATCGA TCGTACGGAA TCTCAGAACG ACCTTTCATA TAGTGATATT 
GTAGTGAGTA TTCGTGTATT GTCAGTGATC GCAGCGATCA GTAAAGCTGT AGATACGGGA 
AATCCAGATT TAGTTTATCA GTCATTAGAA AATCCAGACT GTCATGTTTC TGGTTTGGAT 
CATGAAAACA AAGTGAAGTA TTATAGAGCA CTGTCAGCAG TTCGATGTGA AAAACAAGCA 
ATTGCTGAGG AATGTCCTTT GCTAACGTAC ATCGATATCC AAGAGTGTAT AGATGCCGTA 
AATCAACAGC GTCAAAATGA CGACGATGTA ATACAAGTTT TACGTCAACT AAATGCGGCT 
GTTGTAGAGA ATGATCGAGA CGGAATTATA ATTGCTTTAA AAGATACCGC TTTGAAGTTG 
CAGAAGTTTA CTTCGCCAGA AGATGCATCT CTCTATTTGA AATTATTTAA AAAATGCCTT 
ACTGAGAAAC ATTTTGATGG TTCTGAATTA TGGTTAGAAG ATGTAGAAAC GATAACAAAA 
ATCGTTGCTG CGGAATCTGA AAATGTCCAA AATGCGTGTA CGTTTCTGTC ACAAGTAAAT 
TTAGGTTTAC AACAAAACGA TATGTTATTC ACAGTTGATT GCCTTCAGAC GTTTGGTTTT 
AAAATATCAG ATAAATACAA AACAGAGTGC TTTCAAAGTT TATGTAACTT ACAAAAAATG 
AAGAGTAAAA AGTACAATTG TGCTCTAGTT CGTTATATCA CTCCTAAAGG CAACGAATCG 
TACTTGGATT TGAAAAAGTA TAATTATACT TGGGATTGTC CAAAAAATAT ATCGGAAACA 
TATTATATCA ACATGGAAGA TATCAAGGAA ATAATAAAAA GTACTACCGC AAGAGAACAC 
GAAGAGCATA AAATCAGCAA ACGGATAGTT CGATTGCAAG CTTGCATACG AGGATATTTA 
TTACGAAAAA AAATTTCTGT TAGATTTGCA TATTTTTATG ACAACGTAGA TAAAGTTATT 
AAAATACAAG CCTGGTGGCG CGGTATATTA CAACGTAAAC GATACGCCAA ATTATTAACA 
GAAAGAAGAA ATTATAGTAG TGAAAAATTT TCCAGTAACT GTTTAAGAAT TAAAAATAAG 
TATGCAAATG TATTAGATTA CTATAAACAA CATGAGGAAA AGATAATAAA GATACAAGCT 
TTGTGGCGTG GCCGTGCAGA AAGACGAGCG TTTCACTCAT TGTTGTACAT GGAAAAACCA 
CCATTTCCTG TTGTCCGACA TTTTTCTGCA CTTCTAAATT TTAATGCAGA AGATTATGAC 
AGAGATTTAC AATTACAACA ATTAAAACAC GAAGTGGTAC AAAACATACG GCATAATCAA 
ACTTTATCGC AACAATTGGA TAGTATGGAC ATAAAAATTG GTTTGCTTAT TCAAAATAGG 
ATCACGCTAC AAGATGTTGT AGCACATGGG AAGAGTTTAG AAACACTTGC GAAGCAGAAA 
AATTCAACTA GAGATCAGAA GAATGTTTTA TCAGATAGTA CTATTTCGCA TAAAGGTTTG 
AAATCTTTAA CAAAAGAAGG TCGAAAAATG TTAGAGGGGT ATCAGCATCT ATTCTATGCG 
TTACAAACTA ATCCAACATA TTTATCGAAG ATTTTATTTC TTTTGCCGCA AAGCAAAACG 
AATAAGTTTC TTCAAAATGT TATTTTAACG TTATATAATT TCGGATCAAA TATAAGAGAA 
GAGTATTTAT TATTGAAACT TTTTGGCAGT GCATTACAAG AAGAAATTAG GTGTAAATTT 
CAAAAACCGT CTGAAGTCGT AACTGGAAAT CCACTCGTCT TAAAAATGGT AGTGAATTAC 
GCACGACAAT TACATGGTCA AAGAGCTTTA CGACAAATTG TTGGGCCAAT CATTGAAAAG 
ATATTAGCCG ACCGAACATT GAATATAGAA ACGAATCCCG TTGATATTTA TAAGTGTTGG 
AGGAATCAAT TAGAAATGGA GACTGGAGAA ACACTGAATC TTCCATATAC GGTAACGCAA 
CAACAAGCTT TAAATTACGA ACAGGTACGA ATCAGATTAA ATAAAGGGAT TCAATTGTTG 
CAAAGTACCG TCTTAGAATT CTTAACTAAA ATAACAGAAT CTCGAGATCT AATACCATAC 
GGAATGTTAT ACATGGCGAA AATTTTAAAC GATTCTTTGA CTGAAAAATT TCCAAACGCT 
CCGGAAAAAG ATATTCTCAA AGTAGTTGGA AACTTGATTT ATTATCATTT CATTAACGCA 
GCTATCGTAG CTCCAGATGC TTTCGACATA ATTACCTTAC CGATTGATAG ATCATTGTCA 
AACGATCAAA GGAGAAATTT AGCCAGCATT GCAAAAATAC TACAATTTGC TGCTTCTAAA 
AAGGGGTTTG GAGAGGAAGC CACACATTTA GTGTGTCTGA ACTCTTTTAT AATCGAATGC 
CACGAAAAAT TTAAAAAATT CTTTCGTTTT TGTTGCCAAA TTGAAGATTT GGAAGAACAT 
TTCTGTATTC ATGAGTACAC CGAGGCCACG CTTATTCAGA AGCCTGAGAT CTATATTTCC 
TTACAAGAAA TTTGCGATAC TCACTGCCTC ATGTTAGAGT ATCAAGACCA AATAGCGCCA 
GATCCAATGG ATCCCTTGCA TGATTTGTTA GATGATTTAG GACCTGCACC TACCGTCGCA 
TCTCTGCTAG GAATCTCCGA TACGATGTGT GAGGCAAATT TAATACGGTT TGGAAAAACG 
GAGGTATGTC TGGTACTTAC CAACAAATTT CAAGTACCCG AAGATGAAGA TGCTAGTTTA 
AACAAACTTT TTATAAAAAC TAAAGAGCTA TTGGTTTCTG TACTCCAATT CTTGAAAGGA 
CAAACACTAG TAGAGGCTTT GGAAACCACT TGTTCTCCTG TTCAAGAGAA ACTCTATGAT 
GTTAAGTGTT CTAGTTTATC GCCCACTCTA AGTATCATTC ACAAAAGTTC GTCTTTAAAT 
GATTGTAAAC TTCAACTTCG TGCTTACCTT AATAAACTTG AACTTGGAGG ATGGGTGAGT 
CGATCGGATG GATATCAGAC TATTATAACA GCCGTATCTA AAGATCTTTG TAACAAAGGA 
AAGTACCGAA TCATCCGAAA TAAAGAATTA CAAACTTTAC GCATAACTAA ACAAAGACTG 
GAAGAAAAGT GCAAATATTA CCAGGAACAG GTAGAATATT ATAACGAATA TATACAACGT 
TGCTTGGAGA ACTTGCATAC GGGAAAAGGA TCTTTACGAG CATTTAAAGC CATTCAAAAG 
AATAATCATG GCAAGTTACG GTCTAAAATG ACATTGAAAT ATTCAGCGGC CAAGCTACAA 
GAGAAAGGAA TATTATTAGA AGTGGATGGA CTTCCACAAT CACAATTTAA GAACGTAATT 
TTCGAGATCA GCCCAACAGA GCATACCGGA TTATTTAATG TGCGGTGTAA ATTTATGGGA 
GTAGAAATGG AGAAAGTTGA TATTGATATA CAAAAGCTGC TTGAACTACA ATTTGAGGGT 
TCACCGATTA TGGACATGTT CGGTAAAGCG AAAGTTAACG TGAACTTATT ACTATATTTA 
CTAAATCGAA AATTTTATGG TAAAACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100880131 ( XM_012296340.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012151730.1 Megachile rotundata ras GTPase-activating-like protein IQGAP1 (LOC100880131), transcript variant X2, mRNA.

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CloneID	OMe114318
Clone ID Related Accession (Same CDS sequence)	XM_012296340.1
Accession Version	XM_012296340.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	ras GTPase-activating-like protein IQGAP1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797713.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAATA AATATAATCA AAGAACTGAA CAAGCACCAC GTATTATGAA TGATATTGAT 
GATAAAATAA TTGATCATGA AAAGGTTGAG ACTCGGAAAT CAGCAGAAGA GATGGACGAA 
CAACGTCAAA AAACTCTTGC TTACGAGTAC CTTTGTCACT TAGAGGAAGC TAAAAAATGG 
CTGGAAGCCT GTCTGAGAGA AACTCTCCCA CAAACTATTG AATTAGAAGA AAACTTAAGG 
AACGGGGTAT ATTTAGCAAA ACTTGCACAT TTTATGGCAC CTGATTCTTT ACCCTTTAAT 
AAAATTTATG ACCCTGAACA GAAGCGTTAT ACTGCGGTTG GTTTACAGTT TCGTCATACA 
GATAATATTA ATTATTTTTT AAAATCCTTA AAAACTGTGC AATTACCATT GACTTTCCAA 
CCAGAAACTA CAGACATCTA TGATAAAAAA AATATGCCAC GCGTTATATA TTGCATTCAT 
GCTCTTAGTA CTCATTTATT CAAACTTGGT AGAGCTCCAC AGATTCAGGA TTTGTATGGA 
AAAGTGAACT TCACTGACGA CGAAATAAAT GCTGTTAGTA AAGAATTGAA GAAGTATGGA 
ATTCAAATGC CTTCGTTCCA AAAAATTGGC GGATTATTAG CGAACAGTAT GGAGATCGAT 
GCTGTCACGC TTCATGCTGC GGTAATAGCA ATTAACCAGG CCATCACAGA AAAAGATAAT 
GTTAAAATGT TAATGGCGCT TCAGAATAAA GTAGTGCAAA TAAATAATAT TAACCCTCTC 
TACATTAAGG AATATGGCGA TACATTAACA GAGGCAAAGT ATTCTAAGCA ACAGGCTGCT 
CTTAATAGAT CTCTTAACGA TAGTTATGTG CCAGATATTT ATGATGAATT GTTAACTCAA 
GCAGAAATTC AGGGACATAT TAATTATGTT AATGTCGAGT GCGCAGTGAA AAACATAAGT 
CGGTCAATTC ATCATAACAA TAACGAGTTC GTGAATGCAC TGAGAGCATC TGCTTTGTGT 
TTAAAAAACA TTAAGCATGA AAATGCTGAA TTATATAAAG AACAGTTAAT GCAGTTACTA 
GAAAGTTCTG AATGGAATAA TATGTATTCG GAAAGCAATC ATCATTTGAA ACAATTGCTT 
CAGAAAGAAA TTGATAAATC AAATGAAACA GCGCTTAAAT CTCGGAAACG TGAAGAAGGC 
GTTAAACTCT TAAACTCAAC TTTACAAAAT GGTTTGAAGA ATGAGTTCTC TGAGGCCTTA 
AAAAGTCCGA AATTGGGATT GATCGAACAA ATCGATGATT TTGCAATACC ATTATATTAC 
GAAGAAATGA AAATCGATCG TACGGAATCT CAGAACGACC TTTCATATAG TGATATTGTA 
GTGAGTATTC GTGTATTGTC AGTGATCGCA GCGATCAGTA AAGCTGTAGA TACGGGAAAT 
CCAGATTTAG TTTATCAGTC ATTAGAAAAT CCAGACTGTC ATGTTTCTGG TTTGGATCAT 
GAAAACAAAG TGAAGTATTA TAGAGCACTG TCAGCAGTTC GATGTGAAAA ACAAGCAATT 
GCTGAGGAAT GTCCTTTGCT AACGTACATC GATATCCAAG AGTGTATAGA TGCCGTAAAT 
CAACAGCGTC AAAATGACGA CGATGTAATA CAAGTTTTAC GTCAACTAAA TGCGGCTGTT 
GTAGAGAATG ATCGAGACGG AATTATAATT GCTTTAAAAG ATACCGCTTT GAAGTTGCAG 
AAGTTTACTT CGCCAGAAGA TGCATCTCTC TATTTGAAAT TATTTAAAAA ATGCCTTACT 
GAGAAACATT TTGATGGTTC TGAATTATGG TTAGAAGATG TAGAAACGAT AACAAAAATC 
GTTGCTGCGG AATCTGAAAA TGTCCAAAAT GCGTGTACGT TTCTGTCACA AGTAAATTTA 
GGTTTACAAC AAAACGATAT GTTATTCACA GTTGATTGCC TTCAGACGTT TGGTTTTAAA 
ATATCAGATA AATACAAAAC AGAGTGCTTT CAAAGTTTAT GTAACTTACA AAAAATGAAG 
AGTAAAAAGT ACAATTGTGC TCTAGTTCGT TATATCACTC CTAAAGGCAA CGAATCGTAC 
TTGGATTTGA AAAAGTATAA TTATACTTGG GATTGTCCAA AAAATATATC GGAAACATAT 
TATATCAACA TGGAAGATAT CAAGGAAATA ATAAAAAGTA CTACCGCAAG AGAACACGAA 
GAGCATAAAA TCAGCAAACG GATAGTTCGA TTGCAAGCTT GCATACGAGG ATATTTATTA 
CGAAAAAAAA TTTCTGTTAG ATTTGCATAT TTTTATGACA ACGTAGATAA AGTTATTAAA 
ATACAAGCCT GGTGGCGCGG TATATTACAA CGTAAACGAT ACGCCAAATT ATTAACAGAA 
AGAAGAAATT ATAGTAGTGA AAAATTTTCC AGTAACTGTT TAAGAATTAA AAATAAGTAT 
GCAAATGTAT TAGATTACTA TAAACAACAT GAGGAAAAGA TAATAAAGAT ACAAGCTTTG 
TGGCGTGGCC GTGCAGAAAG ACGAGCGTTT CACTCATTGT TGTACATGGA AAAACCACCA 
TTTCCTGTTG TCCGACATTT TTCTGCACTT CTAAATTTTA ATGCAGAAGA TTATGACAGA 
GATTTACAAT TACAACAATT AAAACACGAA GTGGTACAAA ACATACGGCA TAATCAAACT 
TTATCGCAAC AATTGGATAG TATGGACATA AAAATTGGTT TGCTTATTCA AAATAGGATC 
ACGCTACAAG ATGTTGTAGC ACATGGGAAG AGTTTAGAAA CACTTGCGAA GCAGAAAAAT 
TCAACTAGAG ATCAGAAGAA TGTTTTATCA GATAGTACTA TTTCGCATAA AGGTTTGAAA 
TCTTTAACAA AAGAAGGTCG AAAAATGTTA GAGGGGTATC AGCATCTATT CTATGCGTTA 
CAAACTAATC CAACATATTT ATCGAAGATT TTATTTCTTT TGCCGCAAAG CAAAACGAAT 
AAGTTTCTTC AAAATGTTAT TTTAACGTTA TATAATTTCG GATCAAATAT AAGAGAAGAG 
TATTTATTAT TGAAACTTTT TGGCAGTGCA TTACAAGAAG AAATTAGGTG TAAATTTCAA 
AAACCGTCTG AAGTCGTAAC TGGAAATCCA CTCGTCTTAA AAATGGTAGT GAATTACGCA 
CGACAATTAC ATGGTCAAAG AGCTTTACGA CAAATTGTTG GGCCAATCAT TGAAAAGATA 
TTAGCCGACC GAACATTGAA TATAGAAACG AATCCCGTTG ATATTTATAA GTGTTGGAGG 
AATCAATTAG AAATGGAGAC TGGAGAAACA CTGAATCTTC CATATACGGT AACGCAACAA 
CAAGCTTTAA ATTACGAACA GGTACGAATC AGATTAAATA AAGGGATTCA ATTGTTGCAA 
AGTACCGTCT TAGAATTCTT AACTAAAATA ACAGAATCTC GAGATCTAAT ACCATACGGA 
ATGTTATACA TGGCGAAAAT TTTAAACGAT TCTTTGACTG AAAAATTTCC AAACGCTCCG 
GAAAAAGATA TTCTCAAAGT AGTTGGAAAC TTGATTTATT ATCATTTCAT TAACGCAGCT 
ATCGTAGCTC CAGATGCTTT CGACATAATT ACCTTACCGA TTGATAGATC ATTGTCAAAC 
GATCAAAGGA GAAATTTAGC CAGCATTGCA AAAATACTAC AATTTGCTGC TTCTAAAAAG 
GGGTTTGGAG AGGAAGCCAC ACATTTAGTG TGTCTGAACT CTTTTATAAT CGAATGCCAC 
GAAAAATTTA AAAAATTCTT TCGTTTTTGT TGCCAAATTG AAGATTTGGA AGAACATTTC 
TGTATTCATG AGTACACCGA GGCCACGCTT ATTCAGAAGC CTGAGATCTA TATTTCCTTA 
CAAGAAATTT GCGATACTCA CTGCCTCATG TTAGAGTATC AAGACCAAAT AGCGCCAGAT 
CCAATGGATC CCTTGCATGA TTTGTTAGAT GATTTAGGAC CTGCACCTAC CGTCGCATCT 
CTGCTAGGAA TCTCCGATAC GATGTGTGAG GCAAATTTAA TACGGTTTGG AAAAACGGAG 
GTATGTCTGG TACTTACCAA CAAATTTCAA GTACCCGAAG ATGAAGATGC TAGTTTAAAC 
AAACTTTTTA TAAAAACTAA AGAGCTATTG GTTTCTGTAC TCCAATTCTT GAAAGGACAA 
ACACTAGTAG AGGCTTTGGA AACCACTTGT TCTCCTGTTC AAGAGAAACT CTATGATGTT 
AAGTGTTCTA GTTTATCGCC CACTCTAAGT ATCATTCACA AAAGTTCGTC TTTAAATGAT 
TGTAAACTTC AACTTCGTGC TTACCTTAAT AAACTTGAAC TTGGAGGATG GGTGAGTCGA 
TCGGATGGAT ATCAGACTAT TATAACAGCC GTATCTAAAG ATCTTTGTAA CAAAGGAAAG 
TACCGAATCA TCCGAAATAA AGAATTACAA ACTTTACGCA TAACTAAACA AAGACTGGAA 
GAAAAGTGCA AATATTACCA GGAACAGGTA GAATATTATA ACGAATATAT ACAACGTTGC 
TTGGAGAACT TGCATACGGG AAAAGGATCT TTACGAGCAT TTAAAGCCAT TCAAAAGAAT 
AATCATGGCA AGTTACGGTC TAAAATGACA TTGAAATATT CAGCGGCCAA GCTACAAGAG 
AAAGGAATAT TATTAGAAGT GGATGGACTT CCACAATCAC AATTTAAGAA CGTAATTTTC 
GAGATCAGCC CAACAGAGCA TACCGGATTA TTTAATGTGC GGTGTAAATT TATGGGAGTA 
GAAATGGAGA AAGTTGATAT TGATATACAA AAGCTGCTTG AACTACAATT TGAGGGTTCA 
CCGATTATGG ACATGTTCGG TAAAGCGAAA GTTAACGTGA ACTTATTACT ATATTTACTA 
AATCGAAAAT TTTATGGTAA AACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012151730.1
CDS	34..4980
Translation	MNNKYNQRTEQAPRIMNDIDDKIIDHEKVETRKSAEEMDEQRQKTLAYEYLCHLEEAKKWLEACLRETLPQTIELEENLRNGVYLAKLAHFMAPDSLPFNKIYDPEQKRYTAVGLQFRHTDNINYFLKSLKTVQLPLTFQPETTDIYDKKNMPRVIYCIHALSTHLFKLGRAPQIQDLYGKVNFTDDEINAVSKELKKYGIQMPSFQKIGGLLANSMEIDAVTLHAAVIAINQAITEKDNVKMLMALQNKVVQINNINPLYIKEYGDTLTEAKYSKQQAALNRSLNDSYVPDIYDELLTQAEIQGHINYVNVECAVKNISRSIHHNNNEFVNALRASALCLKNIKHENAELYKEQLMQLLESSEWNNMYSESNHHLKQLLQKEIDKSNETALKSRKREEGVKLLNSTLQNGLKNEFSEALKSPKLGLIEQIDDFAIPLYYEEMKIDRTESQNDLSYSDIVVSIRVLSVIAAISKAVDTGNPDLVYQSLENPDCHVSGLDHENKVKYYRALSAVRCEKQAIAEECPLLTYIDIQECIDAVNQQRQNDDDVIQVLRQLNAAVVENDRDGIIIALKDTALKLQKFTSPEDASLYLKLFKKCLTEKHFDGSELWLEDVETITKIVAAESENVQNACTFLSQVNLGLQQNDMLFTVDCLQTFGFKISDKYKTECFQSLCNLQKMKSKKYNCALVRYITPKGNESYLDLKKYNYTWDCPKNISETYYINMEDIKEIIKSTTAREHEEHKISKRIVRLQACIRGYLLRKKISVRFAYFYDNVDKVIKIQAWWRGILQRKRYAKLLTERRNYSSEKFSSNCLRIKNKYANVLDYYKQHEEKIIKIQALWRGRAERRAFHSLLYMEKPPFPVVRHFSALLNFNAEDYDRDLQLQQLKHEVVQNIRHNQTLSQQLDSMDIKIGLLIQNRITLQDVVAHGKSLETLAKQKNSTRDQKNVLSDSTISHKGLKSLTKEGRKMLEGYQHLFYALQTNPTYLSKILFLLPQSKTNKFLQNVILTLYNFGSNIREEYLLLKLFGSALQEEIRCKFQKPSEVVTGNPLVLKMVVNYARQLHGQRALRQIVGPIIEKILADRTLNIETNPVDIYKCWRNQLEMETGETLNLPYTVTQQQALNYEQVRIRLNKGIQLLQSTVLEFLTKITESRDLIPYGMLYMAKILNDSLTEKFPNAPEKDILKVVGNLIYYHFINAAIVAPDAFDIITLPIDRSLSNDQRRNLASIAKILQFAASKKGFGEEATHLVCLNSFIIECHEKFKKFFRFCCQIEDLEEHFCIHEYTEATLIQKPEIYISLQEICDTHCLMLEYQDQIAPDPMDPLHDLLDDLGPAPTVASLLGISDTMCEANLIRFGKTEVCLVLTNKFQVPEDEDASLNKLFIKTKELLVSVLQFLKGQTLVEALETTCSPVQEKLYDVKCSSLSPTLSIIHKSSSLNDCKLQLRAYLNKLELGGWVSRSDGYQTIITAVSKDLCNKGKYRIIRNKELQTLRITKQRLEEKCKYYQEQVEYYNEYIQRCLENLHTGKGSLRAFKAIQKNNHGKLRSKMTLKYSAAKLQEKGILLEVDGLPQSQFKNVIFEISPTEHTGLFNVRCKFMGVEMEKVDIDIQKLLELQFEGSPIMDMFGKAKVNVNLLLYLLNRKFYGKT

Target ORF information:

RefSeq Version	XM_012296340.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata ras GTPase-activating-like protein IQGAP1 (LOC100880131), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012296340.1

ORF Insert Sequence:

ATGAATAATA AATATAATCA AAGAACTGAA CAAGCACCAC GTATTATGAA TGATATTGAT 
GATAAAATAA TTGATCATGA AAAGGTTGAG ACTCGGAAAT CAGCAGAAGA GATGGACGAA 
CAACGTCAAA AAACTCTTGC TTACGAGTAC CTTTGTCACT TAGAGGAAGC TAAAAAATGG 
CTGGAAGCCT GTCTGAGAGA AACTCTCCCA CAAACTATTG AATTAGAAGA AAACTTAAGG 
AACGGGGTAT ATTTAGCAAA ACTTGCACAT TTTATGGCAC CTGATTCTTT ACCCTTTAAT 
AAAATTTATG ACCCTGAACA GAAGCGTTAT ACTGCGGTTG GTTTACAGTT TCGTCATACA 
GATAATATTA ATTATTTTTT AAAATCCTTA AAAACTGTGC AATTACCATT GACTTTCCAA 
CCAGAAACTA CAGACATCTA TGATAAAAAA AATATGCCAC GCGTTATATA TTGCATTCAT 
GCTCTTAGTA CTCATTTATT CAAACTTGGT AGAGCTCCAC AGATTCAGGA TTTGTATGGA 
AAAGTGAACT TCACTGACGA CGAAATAAAT GCTGTTAGTA AAGAATTGAA GAAGTATGGA 
ATTCAAATGC CTTCGTTCCA AAAAATTGGC GGATTATTAG CGAACAGTAT GGAGATCGAT 
GCTGTCACGC TTCATGCTGC GGTAATAGCA ATTAACCAGG CCATCACAGA AAAAGATAAT 
GTTAAAATGT TAATGGCGCT TCAGAATAAA GTAGTGCAAA TAAATAATAT TAACCCTCTC 
TACATTAAGG AATATGGCGA TACATTAACA GAGGCAAAGT ATTCTAAGCA ACAGGCTGCT 
CTTAATAGAT CTCTTAACGA TAGTTATGTG CCAGATATTT ATGATGAATT GTTAACTCAA 
GCAGAAATTC AGGGACATAT TAATTATGTT AATGTCGAGT GCGCAGTGAA AAACATAAGT 
CGGTCAATTC ATCATAACAA TAACGAGTTC GTGAATGCAC TGAGAGCATC TGCTTTGTGT 
TTAAAAAACA TTAAGCATGA AAATGCTGAA TTATATAAAG AACAGTTAAT GCAGTTACTA 
GAAAGTTCTG AATGGAATAA TATGTATTCG GAAAGCAATC ATCATTTGAA ACAATTGCTT 
CAGAAAGAAA TTGATAAATC AAATGAAACA GCGCTTAAAT CTCGGAAACG TGAAGAAGGC 
GTTAAACTCT TAAACTCAAC TTTACAAAAT GGTTTGAAGA ATGAGTTCTC TGAGGCCTTA 
AAAAGTCCGA AATTGGGATT GATCGAACAA ATCGATGATT TTGCAATACC ATTATATTAC 
GAAGAAATGA AAATCGATCG TACGGAATCT CAGAACGACC TTTCATATAG TGATATTGTA 
GTGAGTATTC GTGTATTGTC AGTGATCGCA GCGATCAGTA AAGCTGTAGA TACGGGAAAT 
CCAGATTTAG TTTATCAGTC ATTAGAAAAT CCAGACTGTC ATGTTTCTGG TTTGGATCAT 
GAAAACAAAG TGAAGTATTA TAGAGCACTG TCAGCAGTTC GATGTGAAAA ACAAGCAATT 
GCTGAGGAAT GTCCTTTGCT AACGTACATC GATATCCAAG AGTGTATAGA TGCCGTAAAT 
CAACAGCGTC AAAATGACGA CGATGTAATA CAAGTTTTAC GTCAACTAAA TGCGGCTGTT 
GTAGAGAATG ATCGAGACGG AATTATAATT GCTTTAAAAG ATACCGCTTT GAAGTTGCAG 
AAGTTTACTT CGCCAGAAGA TGCATCTCTC TATTTGAAAT TATTTAAAAA ATGCCTTACT 
GAGAAACATT TTGATGGTTC TGAATTATGG TTAGAAGATG TAGAAACGAT AACAAAAATC 
GTTGCTGCGG AATCTGAAAA TGTCCAAAAT GCGTGTACGT TTCTGTCACA AGTAAATTTA 
GGTTTACAAC AAAACGATAT GTTATTCACA GTTGATTGCC TTCAGACGTT TGGTTTTAAA 
ATATCAGATA AATACAAAAC AGAGTGCTTT CAAAGTTTAT GTAACTTACA AAAAATGAAG 
AGTAAAAAGT ACAATTGTGC TCTAGTTCGT TATATCACTC CTAAAGGCAA CGAATCGTAC 
TTGGATTTGA AAAAGTATAA TTATACTTGG GATTGTCCAA AAAATATATC GGAAACATAT 
TATATCAACA TGGAAGATAT CAAGGAAATA ATAAAAAGTA CTACCGCAAG AGAACACGAA 
GAGCATAAAA TCAGCAAACG GATAGTTCGA TTGCAAGCTT GCATACGAGG ATATTTATTA 
CGAAAAAAAA TTTCTGTTAG ATTTGCATAT TTTTATGACA ACGTAGATAA AGTTATTAAA 
ATACAAGCCT GGTGGCGCGG TATATTACAA CGTAAACGAT ACGCCAAATT ATTAACAGAA 
AGAAGAAATT ATAGTAGTGA AAAATTTTCC AGTAACTGTT TAAGAATTAA AAATAAGTAT 
GCAAATGTAT TAGATTACTA TAAACAACAT GAGGAAAAGA TAATAAAGAT ACAAGCTTTG 
TGGCGTGGCC GTGCAGAAAG ACGAGCGTTT CACTCATTGT TGTACATGGA AAAACCACCA 
TTTCCTGTTG TCCGACATTT TTCTGCACTT CTAAATTTTA ATGCAGAAGA TTATGACAGA 
GATTTACAAT TACAACAATT AAAACACGAA GTGGTACAAA ACATACGGCA TAATCAAACT 
TTATCGCAAC AATTGGATAG TATGGACATA AAAATTGGTT TGCTTATTCA AAATAGGATC 
ACGCTACAAG ATGTTGTAGC ACATGGGAAG AGTTTAGAAA CACTTGCGAA GCAGAAAAAT 
TCAACTAGAG ATCAGAAGAA TGTTTTATCA GATAGTACTA TTTCGCATAA AGGTTTGAAA 
TCTTTAACAA AAGAAGGTCG AAAAATGTTA GAGGGGTATC AGCATCTATT CTATGCGTTA 
CAAACTAATC CAACATATTT ATCGAAGATT TTATTTCTTT TGCCGCAAAG CAAAACGAAT 
AAGTTTCTTC AAAATGTTAT TTTAACGTTA TATAATTTCG GATCAAATAT AAGAGAAGAG 
TATTTATTAT TGAAACTTTT TGGCAGTGCA TTACAAGAAG AAATTAGGTG TAAATTTCAA 
AAACCGTCTG AAGTCGTAAC TGGAAATCCA CTCGTCTTAA AAATGGTAGT GAATTACGCA 
CGACAATTAC ATGGTCAAAG AGCTTTACGA CAAATTGTTG GGCCAATCAT TGAAAAGATA 
TTAGCCGACC GAACATTGAA TATAGAAACG AATCCCGTTG ATATTTATAA GTGTTGGAGG 
AATCAATTAG AAATGGAGAC TGGAGAAACA CTGAATCTTC CATATACGGT AACGCAACAA 
CAAGCTTTAA ATTACGAACA GGTACGAATC AGATTAAATA AAGGGATTCA ATTGTTGCAA 
AGTACCGTCT TAGAATTCTT AACTAAAATA ACAGAATCTC GAGATCTAAT ACCATACGGA 
ATGTTATACA TGGCGAAAAT TTTAAACGAT TCTTTGACTG AAAAATTTCC AAACGCTCCG 
GAAAAAGATA TTCTCAAAGT AGTTGGAAAC TTGATTTATT ATCATTTCAT TAACGCAGCT 
ATCGTAGCTC CAGATGCTTT CGACATAATT ACCTTACCGA TTGATAGATC ATTGTCAAAC 
GATCAAAGGA GAAATTTAGC CAGCATTGCA AAAATACTAC AATTTGCTGC TTCTAAAAAG 
GGGTTTGGAG AGGAAGCCAC ACATTTAGTG TGTCTGAACT CTTTTATAAT CGAATGCCAC 
GAAAAATTTA AAAAATTCTT TCGTTTTTGT TGCCAAATTG AAGATTTGGA AGAACATTTC 
TGTATTCATG AGTACACCGA GGCCACGCTT ATTCAGAAGC CTGAGATCTA TATTTCCTTA 
CAAGAAATTT GCGATACTCA CTGCCTCATG TTAGAGTATC AAGACCAAAT AGCGCCAGAT 
CCAATGGATC CCTTGCATGA TTTGTTAGAT GATTTAGGAC CTGCACCTAC CGTCGCATCT 
CTGCTAGGAA TCTCCGATAC GATGTGTGAG GCAAATTTAA TACGGTTTGG AAAAACGGAG 
GTATGTCTGG TACTTACCAA CAAATTTCAA GTACCCGAAG ATGAAGATGC TAGTTTAAAC 
AAACTTTTTA TAAAAACTAA AGAGCTATTG GTTTCTGTAC TCCAATTCTT GAAAGGACAA 
ACACTAGTAG AGGCTTTGGA AACCACTTGT TCTCCTGTTC AAGAGAAACT CTATGATGTT 
AAGTGTTCTA GTTTATCGCC CACTCTAAGT ATCATTCACA AAAGTTCGTC TTTAAATGAT 
TGTAAACTTC AACTTCGTGC TTACCTTAAT AAACTTGAAC TTGGAGGATG GGTGAGTCGA 
TCGGATGGAT ATCAGACTAT TATAACAGCC GTATCTAAAG ATCTTTGTAA CAAAGGAAAG 
TACCGAATCA TCCGAAATAA AGAATTACAA ACTTTACGCA TAACTAAACA AAGACTGGAA 
GAAAAGTGCA AATATTACCA GGAACAGGTA GAATATTATA ACGAATATAT ACAACGTTGC 
TTGGAGAACT TGCATACGGG AAAAGGATCT TTACGAGCAT TTAAAGCCAT TCAAAAGAAT 
AATCATGGCA AGTTACGGTC TAAAATGACA TTGAAATATT CAGCGGCCAA GCTACAAGAG 
AAAGGAATAT TATTAGAAGT GGATGGACTT CCACAATCAC AATTTAAGAA CGTAATTTTC 
GAGATCAGCC CAACAGAGCA TACCGGATTA TTTAATGTGC GGTGTAAATT TATGGGAGTA 
GAAATGGAGA AAGTTGATAT TGATATACAA AAGCTGCTTG AACTACAATT TGAGGGTTCA 
CCGATTATGG ACATGTTCGG TAAAGCGAAA GTTAACGTGA ACTTATTACT ATATTTACTA 
AATCGAAAAT TTTATGGTAA AACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100880131 ( XM_012296341.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012151731.1 Megachile rotundata ras GTPase-activating-like protein IQGAP1 (LOC100880131), transcript variant X3, mRNA.

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CloneID	OMe114319
Clone ID Related Accession (Same CDS sequence)	XM_012296341.1
Accession Version	XM_012296341.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	ras GTPase-activating-like protein IQGAP1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797713.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGATA TTGATGATAA AATAATTGAT CATGAAAAGG AAGTTGAGAC TCGGAAATCA 
GCAGAAGAGA TGGACGAACA ACGTCAAAAA ACTCTTGCTT ACGAGTACCT TTGTCACTTA 
GAGGAAGCTA AAAAATGGCT GGAAGCCTGT CTGAGAGAAA CTCTCCCACA AACTATTGAA 
TTAGAAGAAA ACTTAAGGAA CGGGGTATAT TTAGCAAAAC TTGCACATTT TATGGCACCT 
GATTCTTTAC CCTTTAATAA AATTTATGAC CCTGAACAGA AGCGTTATAC TGCGGTTGGT 
TTACAGTTTC GTCATACAGA TAATATTAAT TATTTTTTAA AATCCTTAAA AACTGTGCAA 
TTACCATTGA CTTTCCAACC AGAAACTACA GACATCTATG ATAAAAAAAA TATGCCACGC 
GTTATATATT GCATTCATGC TCTTAGTACT CATTTATTCA AACTTGGTAG AGCTCCACAG 
ATTCAGGATT TGTATGGAAA AGTGAACTTC ACTGACGACG AAATAAATGC TGTTAGTAAA 
GAATTGAAGA AGTATGGAAT TCAAATGCCT TCGTTCCAAA AAATTGGCGG ATTATTAGCG 
AACAGTATGG AGATCGATGC TGTCACGCTT CATGCTGCGG TAATAGCAAT TAACCAGGCC 
ATCACAGAAA AAGATAATGT TAAAATGTTA ATGGCGCTTC AGAATAAAGT AGTGCAAATA 
AATAATATTA ACCCTCTCTA CATTAAGGAA TATGGCGATA CATTAACAGA GGCAAAGTAT 
TCTAAGCAAC AGGCTGCTCT TAATAGATCT CTTAACGATA GTTATGTGCC AGATATTTAT 
GATGAATTGT TAACTCAAGC AGAAATTCAG GGACATATTA ATTATGTTAA TGTCGAGTGC 
GCAGTGAAAA ACATAAGTCG GTCAATTCAT CATAACAATA ACGAGTTCGT GAATGCACTG 
AGAGCATCTG CTTTGTGTTT AAAAAACATT AAGCATGAAA ATGCTGAATT ATATAAAGAA 
CAGTTAATGC AGTTACTAGA AAGTTCTGAA TGGAATAATA TGTATTCGGA AAGCAATCAT 
CATTTGAAAC AATTGCTTCA GAAAGAAATT GATAAATCAA ATGAAACAGC GCTTAAATCT 
CGGAAACGTG AAGAAGGCGT TAAACTCTTA AACTCAACTT TACAAAATGG TTTGAAGAAT 
GAGTTCTCTG AGGCCTTAAA AAGTCCGAAA TTGGGATTGA TCGAACAAAT CGATGATTTT 
GCAATACCAT TATATTACGA AGAAATGAAA ATCGATCGTA CGGAATCTCA GAACGACCTT 
TCATATAGTG ATATTGTAGT GAGTATTCGT GTATTGTCAG TGATCGCAGC GATCAGTAAA 
GCTGTAGATA CGGGAAATCC AGATTTAGTT TATCAGTCAT TAGAAAATCC AGACTGTCAT 
GTTTCTGGTT TGGATCATGA AAACAAAGTG AAGTATTATA GAGCACTGTC AGCAGTTCGA 
TGTGAAAAAC AAGCAATTGC TGAGGAATGT CCTTTGCTAA CGTACATCGA TATCCAAGAG 
TGTATAGATG CCGTAAATCA ACAGCGTCAA AATGACGACG ATGTAATACA AGTTTTACGT 
CAACTAAATG CGGCTGTTGT AGAGAATGAT CGAGACGGAA TTATAATTGC TTTAAAAGAT 
ACCGCTTTGA AGTTGCAGAA GTTTACTTCG CCAGAAGATG CATCTCTCTA TTTGAAATTA 
TTTAAAAAAT GCCTTACTGA GAAACATTTT GATGGTTCTG AATTATGGTT AGAAGATGTA 
GAAACGATAA CAAAAATCGT TGCTGCGGAA TCTGAAAATG TCCAAAATGC GTGTACGTTT 
CTGTCACAAG TAAATTTAGG TTTACAACAA AACGATATGT TATTCACAGT TGATTGCCTT 
CAGACGTTTG GTTTTAAAAT ATCAGATAAA TACAAAACAG AGTGCTTTCA AAGTTTATGT 
AACTTACAAA AAATGAAGAG TAAAAAGTAC AATTGTGCTC TAGTTCGTTA TATCACTCCT 
AAAGGCAACG AATCGTACTT GGATTTGAAA AAGTATAATT ATACTTGGGA TTGTCCAAAA 
AATATATCGG AAACATATTA TATCAACATG GAAGATATCA AGGAAATAAT AAAAAGTACT 
ACCGCAAGAG AACACGAAGA GCATAAAATC AGCAAACGGA TAGTTCGATT GCAAGCTTGC 
ATACGAGGAT ATTTATTACG AAAAAAAATT TCTGTTAGAT TTGCATATTT TTATGACAAC 
GTAGATAAAG TTATTAAAAT ACAAGCCTGG TGGCGCGGTA TATTACAACG TAAACGATAC 
GCCAAATTAT TAACAGAAAG AAGAAATTAT AGTAGTGAAA AATTTTCCAG TAACTGTTTA 
AGAATTAAAA ATAAGTATGC AAATGTATTA GATTACTATA AACAACATGA GGAAAAGATA 
ATAAAGATAC AAGCTTTGTG GCGTGGCCGT GCAGAAAGAC GAGCGTTTCA CTCATTGTTG 
TACATGGAAA AACCACCATT TCCTGTTGTC CGACATTTTT CTGCACTTCT AAATTTTAAT 
GCAGAAGATT ATGACAGAGA TTTACAATTA CAACAATTAA AACACGAAGT GGTACAAAAC 
ATACGGCATA ATCAAACTTT ATCGCAACAA TTGGATAGTA TGGACATAAA AATTGGTTTG 
CTTATTCAAA ATAGGATCAC GCTACAAGAT GTTGTAGCAC ATGGGAAGAG TTTAGAAACA 
CTTGCGAAGC AGAAAAATTC AACTAGAGAT CAGAAGAATG TTTTATCAGA TAGTACTATT 
TCGCATAAAG GTTTGAAATC TTTAACAAAA GAAGGTCGAA AAATGTTAGA GGGGTATCAG 
CATCTATTCT ATGCGTTACA AACTAATCCA ACATATTTAT CGAAGATTTT ATTTCTTTTG 
CCGCAAAGCA AAACGAATAA GTTTCTTCAA AATGTTATTT TAACGTTATA TAATTTCGGA 
TCAAATATAA GAGAAGAGTA TTTATTATTG AAACTTTTTG GCAGTGCATT ACAAGAAGAA 
ATTAGGTGTA AATTTCAAAA ACCGTCTGAA GTCGTAACTG GAAATCCACT CGTCTTAAAA 
ATGGTAGTGA ATTACGCACG ACAATTACAT GGTCAAAGAG CTTTACGACA AATTGTTGGG 
CCAATCATTG AAAAGATATT AGCCGACCGA ACATTGAATA TAGAAACGAA TCCCGTTGAT 
ATTTATAAGT GTTGGAGGAA TCAATTAGAA ATGGAGACTG GAGAAACACT GAATCTTCCA 
TATACGGTAA CGCAACAACA AGCTTTAAAT TACGAACAGG TACGAATCAG ATTAAATAAA 
GGGATTCAAT TGTTGCAAAG TACCGTCTTA GAATTCTTAA CTAAAATAAC AGAATCTCGA 
GATCTAATAC CATACGGAAT GTTATACATG GCGAAAATTT TAAACGATTC TTTGACTGAA 
AAATTTCCAA ACGCTCCGGA AAAAGATATT CTCAAAGTAG TTGGAAACTT GATTTATTAT 
CATTTCATTA ACGCAGCTAT CGTAGCTCCA GATGCTTTCG ACATAATTAC CTTACCGATT 
GATAGATCAT TGTCAAACGA TCAAAGGAGA AATTTAGCCA GCATTGCAAA AATACTACAA 
TTTGCTGCTT CTAAAAAGGG GTTTGGAGAG GAAGCCACAC ATTTAGTGTG TCTGAACTCT 
TTTATAATCG AATGCCACGA AAAATTTAAA AAATTCTTTC GTTTTTGTTG CCAAATTGAA 
GATTTGGAAG AACATTTCTG TATTCATGAG TACACCGAGG CCACGCTTAT TCAGAAGCCT 
GAGATCTATA TTTCCTTACA AGAAATTTGC GATACTCACT GCCTCATGTT AGAGTATCAA 
GACCAAATAG CGCCAGATCC AATGGATCCC TTGCATGATT TGTTAGATGA TTTAGGACCT 
GCACCTACCG TCGCATCTCT GCTAGGAATC TCCGATACGA TGTGTGAGGC AAATTTAATA 
CGGTTTGGAA AAACGGAGGT ATGTCTGGTA CTTACCAACA AATTTCAAGT ACCCGAAGAT 
GAAGATGCTA GTTTAAACAA ACTTTTTATA AAAACTAAAG AGCTATTGGT TTCTGTACTC 
CAATTCTTGA AAGGACAAAC ACTAGTAGAG GCTTTGGAAA CCACTTGTTC TCCTGTTCAA 
GAGAAACTCT ATGATGTTAA GTGTTCTAGT TTATCGCCCA CTCTAAGTAT CATTCACAAA 
AGTTCGTCTT TAAATGATTG TAAACTTCAA CTTCGTGCTT ACCTTAATAA ACTTGAACTT 
GGAGGATGGG TGAGTCGATC GGATGGATAT CAGACTATTA TAACAGCCGT ATCTAAAGAT 
CTTTGTAACA AAGGAAAGTA CCGAATCATC CGAAATAAAG AATTACAAAC TTTACGCATA 
ACTAAACAAA GACTGGAAGA AAAGTGCAAA TATTACCAGG AACAGGTAGA ATATTATAAC 
GAATATATAC AACGTTGCTT GGAGAACTTG CATACGGGAA AAGGATCTTT ACGAGCATTT 
AAAGCCATTC AAAAGAATAA TCATGGCAAG TTACGGTCTA AAATGACATT GAAATATTCA 
GCGGCCAAGC TACAAGAGAA AGGAATATTA TTAGAAGTGG ATGGACTTCC ACAATCACAA 
TTTAAGAACG TAATTTTCGA GATCAGCCCA ACAGAGCATA CCGGATTATT TAATGTGCGG 
TGTAAATTTA TGGGAGTAGA AATGGAGAAA GTTGATATTG ATATACAAAA GCTGCTTGAA 
CTACAATTTG AGGGTTCACC GATTATGGAC ATGTTCGGTA AAGCGAAAGT TAACGTGAAC 
TTATTACTAT ATTTACTAAA TCGAAAATTT TATGGTAAAA CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012151731.1
CDS	100..5004
Translation	MNDIDDKIIDHEKEVETRKSAEEMDEQRQKTLAYEYLCHLEEAKKWLEACLRETLPQTIELEENLRNGVYLAKLAHFMAPDSLPFNKIYDPEQKRYTAVGLQFRHTDNINYFLKSLKTVQLPLTFQPETTDIYDKKNMPRVIYCIHALSTHLFKLGRAPQIQDLYGKVNFTDDEINAVSKELKKYGIQMPSFQKIGGLLANSMEIDAVTLHAAVIAINQAITEKDNVKMLMALQNKVVQINNINPLYIKEYGDTLTEAKYSKQQAALNRSLNDSYVPDIYDELLTQAEIQGHINYVNVECAVKNISRSIHHNNNEFVNALRASALCLKNIKHENAELYKEQLMQLLESSEWNNMYSESNHHLKQLLQKEIDKSNETALKSRKREEGVKLLNSTLQNGLKNEFSEALKSPKLGLIEQIDDFAIPLYYEEMKIDRTESQNDLSYSDIVVSIRVLSVIAAISKAVDTGNPDLVYQSLENPDCHVSGLDHENKVKYYRALSAVRCEKQAIAEECPLLTYIDIQECIDAVNQQRQNDDDVIQVLRQLNAAVVENDRDGIIIALKDTALKLQKFTSPEDASLYLKLFKKCLTEKHFDGSELWLEDVETITKIVAAESENVQNACTFLSQVNLGLQQNDMLFTVDCLQTFGFKISDKYKTECFQSLCNLQKMKSKKYNCALVRYITPKGNESYLDLKKYNYTWDCPKNISETYYINMEDIKEIIKSTTAREHEEHKISKRIVRLQACIRGYLLRKKISVRFAYFYDNVDKVIKIQAWWRGILQRKRYAKLLTERRNYSSEKFSSNCLRIKNKYANVLDYYKQHEEKIIKIQALWRGRAERRAFHSLLYMEKPPFPVVRHFSALLNFNAEDYDRDLQLQQLKHEVVQNIRHNQTLSQQLDSMDIKIGLLIQNRITLQDVVAHGKSLETLAKQKNSTRDQKNVLSDSTISHKGLKSLTKEGRKMLEGYQHLFYALQTNPTYLSKILFLLPQSKTNKFLQNVILTLYNFGSNIREEYLLLKLFGSALQEEIRCKFQKPSEVVTGNPLVLKMVVNYARQLHGQRALRQIVGPIIEKILADRTLNIETNPVDIYKCWRNQLEMETGETLNLPYTVTQQQALNYEQVRIRLNKGIQLLQSTVLEFLTKITESRDLIPYGMLYMAKILNDSLTEKFPNAPEKDILKVVGNLIYYHFINAAIVAPDAFDIITLPIDRSLSNDQRRNLASIAKILQFAASKKGFGEEATHLVCLNSFIIECHEKFKKFFRFCCQIEDLEEHFCIHEYTEATLIQKPEIYISLQEICDTHCLMLEYQDQIAPDPMDPLHDLLDDLGPAPTVASLLGISDTMCEANLIRFGKTEVCLVLTNKFQVPEDEDASLNKLFIKTKELLVSVLQFLKGQTLVEALETTCSPVQEKLYDVKCSSLSPTLSIIHKSSSLNDCKLQLRAYLNKLELGGWVSRSDGYQTIITAVSKDLCNKGKYRIIRNKELQTLRITKQRLEEKCKYYQEQVEYYNEYIQRCLENLHTGKGSLRAFKAIQKNNHGKLRSKMTLKYSAAKLQEKGILLEVDGLPQSQFKNVIFEISPTEHTGLFNVRCKFMGVEMEKVDIDIQKLLELQFEGSPIMDMFGKAKVNVNLLLYLLNRKFYGKT

Target ORF information:

RefSeq Version	XM_012296341.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata ras GTPase-activating-like protein IQGAP1 (LOC100880131), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012296341.1

ORF Insert Sequence:

ATGAATGATA TTGATGATAA AATAATTGAT CATGAAAAGG AAGTTGAGAC TCGGAAATCA 
GCAGAAGAGA TGGACGAACA ACGTCAAAAA ACTCTTGCTT ACGAGTACCT TTGTCACTTA 
GAGGAAGCTA AAAAATGGCT GGAAGCCTGT CTGAGAGAAA CTCTCCCACA AACTATTGAA 
TTAGAAGAAA ACTTAAGGAA CGGGGTATAT TTAGCAAAAC TTGCACATTT TATGGCACCT 
GATTCTTTAC CCTTTAATAA AATTTATGAC CCTGAACAGA AGCGTTATAC TGCGGTTGGT 
TTACAGTTTC GTCATACAGA TAATATTAAT TATTTTTTAA AATCCTTAAA AACTGTGCAA 
TTACCATTGA CTTTCCAACC AGAAACTACA GACATCTATG ATAAAAAAAA TATGCCACGC 
GTTATATATT GCATTCATGC TCTTAGTACT CATTTATTCA AACTTGGTAG AGCTCCACAG 
ATTCAGGATT TGTATGGAAA AGTGAACTTC ACTGACGACG AAATAAATGC TGTTAGTAAA 
GAATTGAAGA AGTATGGAAT TCAAATGCCT TCGTTCCAAA AAATTGGCGG ATTATTAGCG 
AACAGTATGG AGATCGATGC TGTCACGCTT CATGCTGCGG TAATAGCAAT TAACCAGGCC 
ATCACAGAAA AAGATAATGT TAAAATGTTA ATGGCGCTTC AGAATAAAGT AGTGCAAATA 
AATAATATTA ACCCTCTCTA CATTAAGGAA TATGGCGATA CATTAACAGA GGCAAAGTAT 
TCTAAGCAAC AGGCTGCTCT TAATAGATCT CTTAACGATA GTTATGTGCC AGATATTTAT 
GATGAATTGT TAACTCAAGC AGAAATTCAG GGACATATTA ATTATGTTAA TGTCGAGTGC 
GCAGTGAAAA ACATAAGTCG GTCAATTCAT CATAACAATA ACGAGTTCGT GAATGCACTG 
AGAGCATCTG CTTTGTGTTT AAAAAACATT AAGCATGAAA ATGCTGAATT ATATAAAGAA 
CAGTTAATGC AGTTACTAGA AAGTTCTGAA TGGAATAATA TGTATTCGGA AAGCAATCAT 
CATTTGAAAC AATTGCTTCA GAAAGAAATT GATAAATCAA ATGAAACAGC GCTTAAATCT 
CGGAAACGTG AAGAAGGCGT TAAACTCTTA AACTCAACTT TACAAAATGG TTTGAAGAAT 
GAGTTCTCTG AGGCCTTAAA AAGTCCGAAA TTGGGATTGA TCGAACAAAT CGATGATTTT 
GCAATACCAT TATATTACGA AGAAATGAAA ATCGATCGTA CGGAATCTCA GAACGACCTT 
TCATATAGTG ATATTGTAGT GAGTATTCGT GTATTGTCAG TGATCGCAGC GATCAGTAAA 
GCTGTAGATA CGGGAAATCC AGATTTAGTT TATCAGTCAT TAGAAAATCC AGACTGTCAT 
GTTTCTGGTT TGGATCATGA AAACAAAGTG AAGTATTATA GAGCACTGTC AGCAGTTCGA 
TGTGAAAAAC AAGCAATTGC TGAGGAATGT CCTTTGCTAA CGTACATCGA TATCCAAGAG 
TGTATAGATG CCGTAAATCA ACAGCGTCAA AATGACGACG ATGTAATACA AGTTTTACGT 
CAACTAAATG CGGCTGTTGT AGAGAATGAT CGAGACGGAA TTATAATTGC TTTAAAAGAT 
ACCGCTTTGA AGTTGCAGAA GTTTACTTCG CCAGAAGATG CATCTCTCTA TTTGAAATTA 
TTTAAAAAAT GCCTTACTGA GAAACATTTT GATGGTTCTG AATTATGGTT AGAAGATGTA 
GAAACGATAA CAAAAATCGT TGCTGCGGAA TCTGAAAATG TCCAAAATGC GTGTACGTTT 
CTGTCACAAG TAAATTTAGG TTTACAACAA AACGATATGT TATTCACAGT TGATTGCCTT 
CAGACGTTTG GTTTTAAAAT ATCAGATAAA TACAAAACAG AGTGCTTTCA AAGTTTATGT 
AACTTACAAA AAATGAAGAG TAAAAAGTAC AATTGTGCTC TAGTTCGTTA TATCACTCCT 
AAAGGCAACG AATCGTACTT GGATTTGAAA AAGTATAATT ATACTTGGGA TTGTCCAAAA 
AATATATCGG AAACATATTA TATCAACATG GAAGATATCA AGGAAATAAT AAAAAGTACT 
ACCGCAAGAG AACACGAAGA GCATAAAATC AGCAAACGGA TAGTTCGATT GCAAGCTTGC 
ATACGAGGAT ATTTATTACG AAAAAAAATT TCTGTTAGAT TTGCATATTT TTATGACAAC 
GTAGATAAAG TTATTAAAAT ACAAGCCTGG TGGCGCGGTA TATTACAACG TAAACGATAC 
GCCAAATTAT TAACAGAAAG AAGAAATTAT AGTAGTGAAA AATTTTCCAG TAACTGTTTA 
AGAATTAAAA ATAAGTATGC AAATGTATTA GATTACTATA AACAACATGA GGAAAAGATA 
ATAAAGATAC AAGCTTTGTG GCGTGGCCGT GCAGAAAGAC GAGCGTTTCA CTCATTGTTG 
TACATGGAAA AACCACCATT TCCTGTTGTC CGACATTTTT CTGCACTTCT AAATTTTAAT 
GCAGAAGATT ATGACAGAGA TTTACAATTA CAACAATTAA AACACGAAGT GGTACAAAAC 
ATACGGCATA ATCAAACTTT ATCGCAACAA TTGGATAGTA TGGACATAAA AATTGGTTTG 
CTTATTCAAA ATAGGATCAC GCTACAAGAT GTTGTAGCAC ATGGGAAGAG TTTAGAAACA 
CTTGCGAAGC AGAAAAATTC AACTAGAGAT CAGAAGAATG TTTTATCAGA TAGTACTATT 
TCGCATAAAG GTTTGAAATC TTTAACAAAA GAAGGTCGAA AAATGTTAGA GGGGTATCAG 
CATCTATTCT ATGCGTTACA AACTAATCCA ACATATTTAT CGAAGATTTT ATTTCTTTTG 
CCGCAAAGCA AAACGAATAA GTTTCTTCAA AATGTTATTT TAACGTTATA TAATTTCGGA 
TCAAATATAA GAGAAGAGTA TTTATTATTG AAACTTTTTG GCAGTGCATT ACAAGAAGAA 
ATTAGGTGTA AATTTCAAAA ACCGTCTGAA GTCGTAACTG GAAATCCACT CGTCTTAAAA 
ATGGTAGTGA ATTACGCACG ACAATTACAT GGTCAAAGAG CTTTACGACA AATTGTTGGG 
CCAATCATTG AAAAGATATT AGCCGACCGA ACATTGAATA TAGAAACGAA TCCCGTTGAT 
ATTTATAAGT GTTGGAGGAA TCAATTAGAA ATGGAGACTG GAGAAACACT GAATCTTCCA 
TATACGGTAA CGCAACAACA AGCTTTAAAT TACGAACAGG TACGAATCAG ATTAAATAAA 
GGGATTCAAT TGTTGCAAAG TACCGTCTTA GAATTCTTAA CTAAAATAAC AGAATCTCGA 
GATCTAATAC CATACGGAAT GTTATACATG GCGAAAATTT TAAACGATTC TTTGACTGAA 
AAATTTCCAA ACGCTCCGGA AAAAGATATT CTCAAAGTAG TTGGAAACTT GATTTATTAT 
CATTTCATTA ACGCAGCTAT CGTAGCTCCA GATGCTTTCG ACATAATTAC CTTACCGATT 
GATAGATCAT TGTCAAACGA TCAAAGGAGA AATTTAGCCA GCATTGCAAA AATACTACAA 
TTTGCTGCTT CTAAAAAGGG GTTTGGAGAG GAAGCCACAC ATTTAGTGTG TCTGAACTCT 
TTTATAATCG AATGCCACGA AAAATTTAAA AAATTCTTTC GTTTTTGTTG CCAAATTGAA 
GATTTGGAAG AACATTTCTG TATTCATGAG TACACCGAGG CCACGCTTAT TCAGAAGCCT 
GAGATCTATA TTTCCTTACA AGAAATTTGC GATACTCACT GCCTCATGTT AGAGTATCAA 
GACCAAATAG CGCCAGATCC AATGGATCCC TTGCATGATT TGTTAGATGA TTTAGGACCT 
GCACCTACCG TCGCATCTCT GCTAGGAATC TCCGATACGA TGTGTGAGGC AAATTTAATA 
CGGTTTGGAA AAACGGAGGT ATGTCTGGTA CTTACCAACA AATTTCAAGT ACCCGAAGAT 
GAAGATGCTA GTTTAAACAA ACTTTTTATA AAAACTAAAG AGCTATTGGT TTCTGTACTC 
CAATTCTTGA AAGGACAAAC ACTAGTAGAG GCTTTGGAAA CCACTTGTTC TCCTGTTCAA 
GAGAAACTCT ATGATGTTAA GTGTTCTAGT TTATCGCCCA CTCTAAGTAT CATTCACAAA 
AGTTCGTCTT TAAATGATTG TAAACTTCAA CTTCGTGCTT ACCTTAATAA ACTTGAACTT 
GGAGGATGGG TGAGTCGATC GGATGGATAT CAGACTATTA TAACAGCCGT ATCTAAAGAT 
CTTTGTAACA AAGGAAAGTA CCGAATCATC CGAAATAAAG AATTACAAAC TTTACGCATA 
ACTAAACAAA GACTGGAAGA AAAGTGCAAA TATTACCAGG AACAGGTAGA ATATTATAAC 
GAATATATAC AACGTTGCTT GGAGAACTTG CATACGGGAA AAGGATCTTT ACGAGCATTT 
AAAGCCATTC AAAAGAATAA TCATGGCAAG TTACGGTCTA AAATGACATT GAAATATTCA 
GCGGCCAAGC TACAAGAGAA AGGAATATTA TTAGAAGTGG ATGGACTTCC ACAATCACAA 
TTTAAGAACG TAATTTTCGA GATCAGCCCA ACAGAGCATA CCGGATTATT TAATGTGCGG 
TGTAAATTTA TGGGAGTAGA AATGGAGAAA GTTGATATTG ATATACAAAA GCTGCTTGAA 
CTACAATTTG AGGGTTCACC GATTATGGAC ATGTTCGGTA AAGCGAAAGT TAACGTGAAC 
TTATTACTAT ATTTACTAAA TCGAAAATTT TATGGTAAAA CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100880131 ( XM_012296342.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012151732.1 Megachile rotundata ras GTPase-activating-like protein IQGAP1 (LOC100880131), transcript variant X4, mRNA.

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CloneID	OMe114320
Clone ID Related Accession (Same CDS sequence)	XM_012296342.1
Accession Version	XM_012296342.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	ras GTPase-activating-like protein IQGAP1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797713.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAATA AATATAATCA AAGAACTGAA CAAGCACCAC GTATTATGAA TGATATTGAT 
GATAAAATAA TTGATCATGA AAAGGAAGTT GAGACTCGGA AATCAGCAGA AGAGATGGAC 
GAACAACGTC AAAAAACTCT TGCTTACGAG TACCTTTGTC ACTTAGAGGA AGCTAAAAAA 
TGGCTGGAAG CCTGTCTGAG AGAAACTCTC CCACAAACTA TTGAATTAGA AGAAAACTTA 
AGGAACGGGG TATATTTAGC AAAACTTGCA CATTTTATGG CACCTGATTC TTTACCCTTT 
AATAAAATTT ATGACCCTGA ACAGAAGCGT TATACTGCGG TTGGTTTACA GTTTCGTCAT 
ACAGATAATA TTAATTATTT TTTAAAATCC TTAAAAACTG TGCAATTACC ATTGACTTTC 
CAACCAGAAA CTACAGACAT CTATGATAAA AAAAATATGC CACGCGTTAT ATATTGCATT 
CATGCTCTTA GTACTCATTT ATTCAAACTT GGTAGAGCTC CACAGATTCA GGATTTGTAT 
GGAAAAGTGA ACTTCACTGA CGACGAAATA AATGCTGTTA GTAAAGAATT GAAGAAGTAT 
GGAATTCAAA TGCCTTCGTT CCAAAAAATT GGCGGATTAT TAGCGAACAG TATGGAGATC 
GATGCTGTCA CGCTTCATGC TGCGGTAATA GCAATTAACC AGGCCATCAC AGAAAAAGAT 
AATGTTAAAA TGTTAATGGC GCTTCAGAAT AAAGTAGTGC AAATAAATAA TATTAACCCT 
CTCTACATTA AGGAATATGG CGATACATTA ACAGAGGCAA AGTATTCTAA GCAACAGGCT 
GCTCTTAATA GATCTCTTAA CGATAGTTAT GTGCCAGATA TTTATGATGA ATTGTTAACT 
CAAGCAGAAA TTCAGGGACA TATTAATTAT GTTAATGTCG AGTGCGCAGT GAAAAACATA 
AGTCGGTCAA TTCATCATAA CAATAACGAG TTCGTGAATG CACTGAGAGC ATCTGCTTTG 
TGTTTAAAAA ACATTAAGCA TGAAAATGCT GAATTATATA AAGAACAGTT AATGCAGTTA 
CTAGAAAGTT CTGAATGGAA TAATATGTAT TCGGAAAGCA ATCATCATTT GAAACAATTG 
CTTCAGAAAG AAATTGATAA ATCAAATGAA ACAGCGCTTA AATCTCGGAA ACGTGAAGAA 
GGCGTTAAAC TCTTAAACTC AACTTTACAA AATGGTTTGA AGAATGAGTT CTCTGAGGCC 
TTAAAAAGTC CGAAATTGGG ATTGATCGAA CAAATCGATG ATTTTGCAAT ACCATTATAT 
TACGAAGAAA TGAAAATCGA TCGTACGGAA TCTCAGAACG ACCTTTCATA TAGTGATATT 
GTAGGTTTGG ATCATGAAAA CAAAGTGAAG TATTATAGAG CACTGTCAGC AGTTCGATGT 
GAAAAACAAG CAATTGCTGA GGAATGTCCT TTGCTAACGT ACATCGATAT CCAAGAGTGT 
ATAGATGCCG TAAATCAACA GCGTCAAAAT GACGACGATG TAATACAAGT TTTACGTCAA 
CTAAATGCGG CTGTTGTAGA GAATGATCGA GACGGAATTA TAATTGCTTT AAAAGATACC 
GCTTTGAAGT TGCAGAAGTT TACTTCGCCA GAAGATGCAT CTCTCTATTT GAAATTATTT 
AAAAAATGCC TTACTGAGAA ACATTTTGAT GGTTCTGAAT TATGGTTAGA AGATGTAGAA 
ACGATAACAA AAATCGTTGC TGCGGAATCT GAAAATGTCC AAAATGCGTG TACGTTTCTG 
TCACAAGTAA ATTTAGGTTT ACAACAAAAC GATATGTTAT TCACAGTTGA TTGCCTTCAG 
ACGTTTGGTT TTAAAATATC AGATAAATAC AAAACAGAGT GCTTTCAAAG TTTATGTAAC 
TTACAAAAAA TGAAGAGTAA AAAGTACAAT TGTGCTCTAG TTCGTTATAT CACTCCTAAA 
GGCAACGAAT CGTACTTGGA TTTGAAAAAG TATAATTATA CTTGGGATTG TCCAAAAAAT 
ATATCGGAAA CATATTATAT CAACATGGAA GATATCAAGG AAATAATAAA AAGTACTACC 
GCAAGAGAAC ACGAAGAGCA TAAAATCAGC AAACGGATAG TTCGATTGCA AGCTTGCATA 
CGAGGATATT TATTACGAAA AAAAATTTCT GTTAGATTTG CATATTTTTA TGACAACGTA 
GATAAAGTTA TTAAAATACA AGCCTGGTGG CGCGGTATAT TACAACGTAA ACGATACGCC 
AAATTATTAA CAGAAAGAAG AAATTATAGT AGTGAAAAAT TTTCCAGTAA CTGTTTAAGA 
ATTAAAAATA AGTATGCAAA TGTATTAGAT TACTATAAAC AACATGAGGA AAAGATAATA 
AAGATACAAG CTTTGTGGCG TGGCCGTGCA GAAAGACGAG CGTTTCACTC ATTGTTGTAC 
ATGGAAAAAC CACCATTTCC TGTTGTCCGA CATTTTTCTG CACTTCTAAA TTTTAATGCA 
GAAGATTATG ACAGAGATTT ACAATTACAA CAATTAAAAC ACGAAGTGGT ACAAAACATA 
CGGCATAATC AAACTTTATC GCAACAATTG GATAGTATGG ACATAAAAAT TGGTTTGCTT 
ATTCAAAATA GGATCACGCT ACAAGATGTT GTAGCACATG GGAAGAGTTT AGAAACACTT 
GCGAAGCAGA AAAATTCAAC TAGAGATCAG AAGAATGTTT TATCAGATAG TACTATTTCG 
CATAAAGGTT TGAAATCTTT AACAAAAGAA GGTCGAAAAA TGTTAGAGGG GTATCAGCAT 
CTATTCTATG CGTTACAAAC TAATCCAACA TATTTATCGA AGATTTTATT TCTTTTGCCG 
CAAAGCAAAA CGAATAAGTT TCTTCAAAAT GTTATTTTAA CGTTATATAA TTTCGGATCA 
AATATAAGAG AAGAGTATTT ATTATTGAAA CTTTTTGGCA GTGCATTACA AGAAGAAATT 
AGGTGTAAAT TTCAAAAACC GTCTGAAGTC GTAACTGGAA ATCCACTCGT CTTAAAAATG 
GTAGTGAATT ACGCACGACA ATTACATGGT CAAAGAGCTT TACGACAAAT TGTTGGGCCA 
ATCATTGAAA AGATATTAGC CGACCGAACA TTGAATATAG AAACGAATCC CGTTGATATT 
TATAAGTGTT GGAGGAATCA ATTAGAAATG GAGACTGGAG AAACACTGAA TCTTCCATAT 
ACGGTAACGC AACAACAAGC TTTAAATTAC GAACAGGTAC GAATCAGATT AAATAAAGGG 
ATTCAATTGT TGCAAAGTAC CGTCTTAGAA TTCTTAACTA AAATAACAGA ATCTCGAGAT 
CTAATACCAT ACGGAATGTT ATACATGGCG AAAATTTTAA ACGATTCTTT GACTGAAAAA 
TTTCCAAACG CTCCGGAAAA AGATATTCTC AAAGTAGTTG GAAACTTGAT TTATTATCAT 
TTCATTAACG CAGCTATCGT AGCTCCAGAT GCTTTCGACA TAATTACCTT ACCGATTGAT 
AGATCATTGT CAAACGATCA AAGGAGAAAT TTAGCCAGCA TTGCAAAAAT ACTACAATTT 
GCTGCTTCTA AAAAGGGGTT TGGAGAGGAA GCCACACATT TAGTGTGTCT GAACTCTTTT 
ATAATCGAAT GCCACGAAAA ATTTAAAAAA TTCTTTCGTT TTTGTTGCCA AATTGAAGAT 
TTGGAAGAAC ATTTCTGTAT TCATGAGTAC ACCGAGGCCA CGCTTATTCA GAAGCCTGAG 
ATCTATATTT CCTTACAAGA AATTTGCGAT ACTCACTGCC TCATGTTAGA GTATCAAGAC 
CAAATAGCGC CAGATCCAAT GGATCCCTTG CATGATTTGT TAGATGATTT AGGACCTGCA 
CCTACCGTCG CATCTCTGCT AGGAATCTCC GATACGATGT GTGAGGCAAA TTTAATACGG 
TTTGGAAAAA CGGAGGTATG TCTGGTACTT ACCAACAAAT TTCAAGTACC CGAAGATGAA 
GATGCTAGTT TAAACAAACT TTTTATAAAA ACTAAAGAGC TATTGGTTTC TGTACTCCAA 
TTCTTGAAAG GACAAACACT AGTAGAGGCT TTGGAAACCA CTTGTTCTCC TGTTCAAGAG 
AAACTCTATG ATGTTAAGTG TTCTAGTTTA TCGCCCACTC TAAGTATCAT TCACAAAAGT 
TCGTCTTTAA ATGATTGTAA ACTTCAACTT CGTGCTTACC TTAATAAACT TGAACTTGGA 
GGATGGGTGA GTCGATCGGA TGGATATCAG ACTATTATAA CAGCCGTATC TAAAGATCTT 
TGTAACAAAG GAAAGTACCG AATCATCCGA AATAAAGAAT TACAAACTTT ACGCATAACT 
AAACAAAGAC TGGAAGAAAA GTGCAAATAT TACCAGGAAC AGGTAGAATA TTATAACGAA 
TATATACAAC GTTGCTTGGA GAACTTGCAT ACGGGAAAAG GATCTTTACG AGCATTTAAA 
GCCATTCAAA AGAATAATCA TGGCAAGTTA CGGTCTAAAA TGACATTGAA ATATTCAGCG 
GCCAAGCTAC AAGAGAAAGG AATATTATTA GAAGTGGATG GACTTCCACA ATCACAATTT 
AAGAACGTAA TTTTCGAGAT CAGCCCAACA GAGCATACCG GATTATTTAA TGTGCGGTGT 
AAATTTATGG GAGTAGAAAT GGAGAAAGTT GATATTGATA TACAAAAGCT GCTTGAACTA 
CAATTTGAGG GTTCACCGAT TATGGACATG TTCGGTAAAG CGAAAGTTAA CGTGAACTTA 
TTACTATATT TACTAAATCG AAAATTTTAT GGTAAAACTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012151732.1
CDS	34..4875
Translation	MNNKYNQRTEQAPRIMNDIDDKIIDHEKEVETRKSAEEMDEQRQKTLAYEYLCHLEEAKKWLEACLRETLPQTIELEENLRNGVYLAKLAHFMAPDSLPFNKIYDPEQKRYTAVGLQFRHTDNINYFLKSLKTVQLPLTFQPETTDIYDKKNMPRVIYCIHALSTHLFKLGRAPQIQDLYGKVNFTDDEINAVSKELKKYGIQMPSFQKIGGLLANSMEIDAVTLHAAVIAINQAITEKDNVKMLMALQNKVVQINNINPLYIKEYGDTLTEAKYSKQQAALNRSLNDSYVPDIYDELLTQAEIQGHINYVNVECAVKNISRSIHHNNNEFVNALRASALCLKNIKHENAELYKEQLMQLLESSEWNNMYSESNHHLKQLLQKEIDKSNETALKSRKREEGVKLLNSTLQNGLKNEFSEALKSPKLGLIEQIDDFAIPLYYEEMKIDRTESQNDLSYSDIVGLDHENKVKYYRALSAVRCEKQAIAEECPLLTYIDIQECIDAVNQQRQNDDDVIQVLRQLNAAVVENDRDGIIIALKDTALKLQKFTSPEDASLYLKLFKKCLTEKHFDGSELWLEDVETITKIVAAESENVQNACTFLSQVNLGLQQNDMLFTVDCLQTFGFKISDKYKTECFQSLCNLQKMKSKKYNCALVRYITPKGNESYLDLKKYNYTWDCPKNISETYYINMEDIKEIIKSTTAREHEEHKISKRIVRLQACIRGYLLRKKISVRFAYFYDNVDKVIKIQAWWRGILQRKRYAKLLTERRNYSSEKFSSNCLRIKNKYANVLDYYKQHEEKIIKIQALWRGRAERRAFHSLLYMEKPPFPVVRHFSALLNFNAEDYDRDLQLQQLKHEVVQNIRHNQTLSQQLDSMDIKIGLLIQNRITLQDVVAHGKSLETLAKQKNSTRDQKNVLSDSTISHKGLKSLTKEGRKMLEGYQHLFYALQTNPTYLSKILFLLPQSKTNKFLQNVILTLYNFGSNIREEYLLLKLFGSALQEEIRCKFQKPSEVVTGNPLVLKMVVNYARQLHGQRALRQIVGPIIEKILADRTLNIETNPVDIYKCWRNQLEMETGETLNLPYTVTQQQALNYEQVRIRLNKGIQLLQSTVLEFLTKITESRDLIPYGMLYMAKILNDSLTEKFPNAPEKDILKVVGNLIYYHFINAAIVAPDAFDIITLPIDRSLSNDQRRNLASIAKILQFAASKKGFGEEATHLVCLNSFIIECHEKFKKFFRFCCQIEDLEEHFCIHEYTEATLIQKPEIYISLQEICDTHCLMLEYQDQIAPDPMDPLHDLLDDLGPAPTVASLLGISDTMCEANLIRFGKTEVCLVLTNKFQVPEDEDASLNKLFIKTKELLVSVLQFLKGQTLVEALETTCSPVQEKLYDVKCSSLSPTLSIIHKSSSLNDCKLQLRAYLNKLELGGWVSRSDGYQTIITAVSKDLCNKGKYRIIRNKELQTLRITKQRLEEKCKYYQEQVEYYNEYIQRCLENLHTGKGSLRAFKAIQKNNHGKLRSKMTLKYSAAKLQEKGILLEVDGLPQSQFKNVIFEISPTEHTGLFNVRCKFMGVEMEKVDIDIQKLLELQFEGSPIMDMFGKAKVNVNLLLYLLNRKFYGKT

Target ORF information:

RefSeq Version	XM_012296342.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata ras GTPase-activating-like protein IQGAP1 (LOC100880131), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012296342.1

ORF Insert Sequence:

ATGAATAATA AATATAATCA AAGAACTGAA CAAGCACCAC GTATTATGAA TGATATTGAT 
GATAAAATAA TTGATCATGA AAAGGAAGTT GAGACTCGGA AATCAGCAGA AGAGATGGAC 
GAACAACGTC AAAAAACTCT TGCTTACGAG TACCTTTGTC ACTTAGAGGA AGCTAAAAAA 
TGGCTGGAAG CCTGTCTGAG AGAAACTCTC CCACAAACTA TTGAATTAGA AGAAAACTTA 
AGGAACGGGG TATATTTAGC AAAACTTGCA CATTTTATGG CACCTGATTC TTTACCCTTT 
AATAAAATTT ATGACCCTGA ACAGAAGCGT TATACTGCGG TTGGTTTACA GTTTCGTCAT 
ACAGATAATA TTAATTATTT TTTAAAATCC TTAAAAACTG TGCAATTACC ATTGACTTTC 
CAACCAGAAA CTACAGACAT CTATGATAAA AAAAATATGC CACGCGTTAT ATATTGCATT 
CATGCTCTTA GTACTCATTT ATTCAAACTT GGTAGAGCTC CACAGATTCA GGATTTGTAT 
GGAAAAGTGA ACTTCACTGA CGACGAAATA AATGCTGTTA GTAAAGAATT GAAGAAGTAT 
GGAATTCAAA TGCCTTCGTT CCAAAAAATT GGCGGATTAT TAGCGAACAG TATGGAGATC 
GATGCTGTCA CGCTTCATGC TGCGGTAATA GCAATTAACC AGGCCATCAC AGAAAAAGAT 
AATGTTAAAA TGTTAATGGC GCTTCAGAAT AAAGTAGTGC AAATAAATAA TATTAACCCT 
CTCTACATTA AGGAATATGG CGATACATTA ACAGAGGCAA AGTATTCTAA GCAACAGGCT 
GCTCTTAATA GATCTCTTAA CGATAGTTAT GTGCCAGATA TTTATGATGA ATTGTTAACT 
CAAGCAGAAA TTCAGGGACA TATTAATTAT GTTAATGTCG AGTGCGCAGT GAAAAACATA 
AGTCGGTCAA TTCATCATAA CAATAACGAG TTCGTGAATG CACTGAGAGC ATCTGCTTTG 
TGTTTAAAAA ACATTAAGCA TGAAAATGCT GAATTATATA AAGAACAGTT AATGCAGTTA 
CTAGAAAGTT CTGAATGGAA TAATATGTAT TCGGAAAGCA ATCATCATTT GAAACAATTG 
CTTCAGAAAG AAATTGATAA ATCAAATGAA ACAGCGCTTA AATCTCGGAA ACGTGAAGAA 
GGCGTTAAAC TCTTAAACTC AACTTTACAA AATGGTTTGA AGAATGAGTT CTCTGAGGCC 
TTAAAAAGTC CGAAATTGGG ATTGATCGAA CAAATCGATG ATTTTGCAAT ACCATTATAT 
TACGAAGAAA TGAAAATCGA TCGTACGGAA TCTCAGAACG ACCTTTCATA TAGTGATATT 
GTAGGTTTGG ATCATGAAAA CAAAGTGAAG TATTATAGAG CACTGTCAGC AGTTCGATGT 
GAAAAACAAG CAATTGCTGA GGAATGTCCT TTGCTAACGT ACATCGATAT CCAAGAGTGT 
ATAGATGCCG TAAATCAACA GCGTCAAAAT GACGACGATG TAATACAAGT TTTACGTCAA 
CTAAATGCGG CTGTTGTAGA GAATGATCGA GACGGAATTA TAATTGCTTT AAAAGATACC 
GCTTTGAAGT TGCAGAAGTT TACTTCGCCA GAAGATGCAT CTCTCTATTT GAAATTATTT 
AAAAAATGCC TTACTGAGAA ACATTTTGAT GGTTCTGAAT TATGGTTAGA AGATGTAGAA 
ACGATAACAA AAATCGTTGC TGCGGAATCT GAAAATGTCC AAAATGCGTG TACGTTTCTG 
TCACAAGTAA ATTTAGGTTT ACAACAAAAC GATATGTTAT TCACAGTTGA TTGCCTTCAG 
ACGTTTGGTT TTAAAATATC AGATAAATAC AAAACAGAGT GCTTTCAAAG TTTATGTAAC 
TTACAAAAAA TGAAGAGTAA AAAGTACAAT TGTGCTCTAG TTCGTTATAT CACTCCTAAA 
GGCAACGAAT CGTACTTGGA TTTGAAAAAG TATAATTATA CTTGGGATTG TCCAAAAAAT 
ATATCGGAAA CATATTATAT CAACATGGAA GATATCAAGG AAATAATAAA AAGTACTACC 
GCAAGAGAAC ACGAAGAGCA TAAAATCAGC AAACGGATAG TTCGATTGCA AGCTTGCATA 
CGAGGATATT TATTACGAAA AAAAATTTCT GTTAGATTTG CATATTTTTA TGACAACGTA 
GATAAAGTTA TTAAAATACA AGCCTGGTGG CGCGGTATAT TACAACGTAA ACGATACGCC 
AAATTATTAA CAGAAAGAAG AAATTATAGT AGTGAAAAAT TTTCCAGTAA CTGTTTAAGA 
ATTAAAAATA AGTATGCAAA TGTATTAGAT TACTATAAAC AACATGAGGA AAAGATAATA 
AAGATACAAG CTTTGTGGCG TGGCCGTGCA GAAAGACGAG CGTTTCACTC ATTGTTGTAC 
ATGGAAAAAC CACCATTTCC TGTTGTCCGA CATTTTTCTG CACTTCTAAA TTTTAATGCA 
GAAGATTATG ACAGAGATTT ACAATTACAA CAATTAAAAC ACGAAGTGGT ACAAAACATA 
CGGCATAATC AAACTTTATC GCAACAATTG GATAGTATGG ACATAAAAAT TGGTTTGCTT 
ATTCAAAATA GGATCACGCT ACAAGATGTT GTAGCACATG GGAAGAGTTT AGAAACACTT 
GCGAAGCAGA AAAATTCAAC TAGAGATCAG AAGAATGTTT TATCAGATAG TACTATTTCG 
CATAAAGGTT TGAAATCTTT AACAAAAGAA GGTCGAAAAA TGTTAGAGGG GTATCAGCAT 
CTATTCTATG CGTTACAAAC TAATCCAACA TATTTATCGA AGATTTTATT TCTTTTGCCG 
CAAAGCAAAA CGAATAAGTT TCTTCAAAAT GTTATTTTAA CGTTATATAA TTTCGGATCA 
AATATAAGAG AAGAGTATTT ATTATTGAAA CTTTTTGGCA GTGCATTACA AGAAGAAATT 
AGGTGTAAAT TTCAAAAACC GTCTGAAGTC GTAACTGGAA ATCCACTCGT CTTAAAAATG 
GTAGTGAATT ACGCACGACA ATTACATGGT CAAAGAGCTT TACGACAAAT TGTTGGGCCA 
ATCATTGAAA AGATATTAGC CGACCGAACA TTGAATATAG AAACGAATCC CGTTGATATT 
TATAAGTGTT GGAGGAATCA ATTAGAAATG GAGACTGGAG AAACACTGAA TCTTCCATAT 
ACGGTAACGC AACAACAAGC TTTAAATTAC GAACAGGTAC GAATCAGATT AAATAAAGGG 
ATTCAATTGT TGCAAAGTAC CGTCTTAGAA TTCTTAACTA AAATAACAGA ATCTCGAGAT 
CTAATACCAT ACGGAATGTT ATACATGGCG AAAATTTTAA ACGATTCTTT GACTGAAAAA 
TTTCCAAACG CTCCGGAAAA AGATATTCTC AAAGTAGTTG GAAACTTGAT TTATTATCAT 
TTCATTAACG CAGCTATCGT AGCTCCAGAT GCTTTCGACA TAATTACCTT ACCGATTGAT 
AGATCATTGT CAAACGATCA AAGGAGAAAT TTAGCCAGCA TTGCAAAAAT ACTACAATTT 
GCTGCTTCTA AAAAGGGGTT TGGAGAGGAA GCCACACATT TAGTGTGTCT GAACTCTTTT 
ATAATCGAAT GCCACGAAAA ATTTAAAAAA TTCTTTCGTT TTTGTTGCCA AATTGAAGAT 
TTGGAAGAAC ATTTCTGTAT TCATGAGTAC ACCGAGGCCA CGCTTATTCA GAAGCCTGAG 
ATCTATATTT CCTTACAAGA AATTTGCGAT ACTCACTGCC TCATGTTAGA GTATCAAGAC 
CAAATAGCGC CAGATCCAAT GGATCCCTTG CATGATTTGT TAGATGATTT AGGACCTGCA 
CCTACCGTCG CATCTCTGCT AGGAATCTCC GATACGATGT GTGAGGCAAA TTTAATACGG 
TTTGGAAAAA CGGAGGTATG TCTGGTACTT ACCAACAAAT TTCAAGTACC CGAAGATGAA 
GATGCTAGTT TAAACAAACT TTTTATAAAA ACTAAAGAGC TATTGGTTTC TGTACTCCAA 
TTCTTGAAAG GACAAACACT AGTAGAGGCT TTGGAAACCA CTTGTTCTCC TGTTCAAGAG 
AAACTCTATG ATGTTAAGTG TTCTAGTTTA TCGCCCACTC TAAGTATCAT TCACAAAAGT 
TCGTCTTTAA ATGATTGTAA ACTTCAACTT CGTGCTTACC TTAATAAACT TGAACTTGGA 
GGATGGGTGA GTCGATCGGA TGGATATCAG ACTATTATAA CAGCCGTATC TAAAGATCTT 
TGTAACAAAG GAAAGTACCG AATCATCCGA AATAAAGAAT TACAAACTTT ACGCATAACT 
AAACAAAGAC TGGAAGAAAA GTGCAAATAT TACCAGGAAC AGGTAGAATA TTATAACGAA 
TATATACAAC GTTGCTTGGA GAACTTGCAT ACGGGAAAAG GATCTTTACG AGCATTTAAA 
GCCATTCAAA AGAATAATCA TGGCAAGTTA CGGTCTAAAA TGACATTGAA ATATTCAGCG 
GCCAAGCTAC AAGAGAAAGG AATATTATTA GAAGTGGATG GACTTCCACA ATCACAATTT 
AAGAACGTAA TTTTCGAGAT CAGCCCAACA GAGCATACCG GATTATTTAA TGTGCGGTGT 
AAATTTATGG GAGTAGAAAT GGAGAAAGTT GATATTGATA TACAAAAGCT GCTTGAACTA 
CAATTTGAGG GTTCACCGAT TATGGACATG TTCGGTAAAG CGAAAGTTAA CGTGAACTTA 
TTACTATATT TACTAAATCG AAAATTTTAT GGTAAAACTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100880131 ( XM_012296343.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012151733.1 Megachile rotundata ras GTPase-activating-like protein IQGAP1 (LOC100880131), transcript variant X5, mRNA.

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CloneID	OMe114321
Clone ID Related Accession (Same CDS sequence)	XM_012296343.1
Accession Version	XM_012296343.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4836bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	ras GTPase-activating-like protein IQGAP1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797713.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAAC AACGTCAAAA AACTCTTGCT TACGAGTACC TTTGTCACTT AGAGGAAGCT 
AAAAAATGGC TGGAAGCCTG TCTGAGAGAA ACTCTCCCAC AAACTATTGA ATTAGAAGAA 
AACTTAAGGA ACGGGGTATA TTTAGCAAAA CTTGCACATT TTATGGCACC TGATTCTTTA 
CCCTTTAATA AAATTTATGA CCCTGAACAG AAGCGTTATA CTGCGGTTGG TTTACAGTTT 
CGTCATACAG ATAATATTAA TTATTTTTTA AAATCCTTAA AAACTGTGCA ATTACCATTG 
ACTTTCCAAC CAGAAACTAC AGACATCTAT GATAAAAAAA ATATGCCACG CGTTATATAT 
TGCATTCATG CTCTTAGTAC TCATTTATTC AAACTTGGTA GAGCTCCACA GATTCAGGAT 
TTGTATGGAA AAGTGAACTT CACTGACGAC GAAATAAATG CTGTTAGTAA AGAATTGAAG 
AAGTATGGAA TTCAAATGCC TTCGTTCCAA AAAATTGGCG GATTATTAGC GAACAGTATG 
GAGATCGATG CTGTCACGCT TCATGCTGCG GTAATAGCAA TTAACCAGGC CATCACAGAA 
AAAGATAATG TTAAAATGTT AATGGCGCTT CAGAATAAAG TAGTGCAAAT AAATAATATT 
AACCCTCTCT ACATTAAGGA ATATGGCGAT ACATTAACAG AGGCAAAGTA TTCTAAGCAA 
CAGGCTGCTC TTAATAGATC TCTTAACGAT AGTTATGTGC CAGATATTTA TGATGAATTG 
TTAACTCAAG CAGAAATTCA GGGACATATT AATTATGTTA ATGTCGAGTG CGCAGTGAAA 
AACATAAGTC GGTCAATTCA TCATAACAAT AACGAGTTCG TGAATGCACT GAGAGCATCT 
GCTTTGTGTT TAAAAAACAT TAAGCATGAA AATGCTGAAT TATATAAAGA ACAGTTAATG 
CAGTTACTAG AAAGTTCTGA ATGGAATAAT ATGTATTCGG AAAGCAATCA TCATTTGAAA 
CAATTGCTTC AGAAAGAAAT TGATAAATCA AATGAAACAG CGCTTAAATC TCGGAAACGT 
GAAGAAGGCG TTAAACTCTT AAACTCAACT TTACAAAATG GTTTGAAGAA TGAGTTCTCT 
GAGGCCTTAA AAAGTCCGAA ATTGGGATTG ATCGAACAAA TCGATGATTT TGCAATACCA 
TTATATTACG AAGAAATGAA AATCGATCGT ACGGAATCTC AGAACGACCT TTCATATAGT 
GATATTGTAG TGAGTATTCG TGTATTGTCA GTGATCGCAG CGATCAGTAA AGCTGTAGAT 
ACGGGAAATC CAGATTTAGT TTATCAGTCA TTAGAAAATC CAGACTGTCA TGTTTCTGGT 
TTGGATCATG AAAACAAAGT GAAGTATTAT AGAGCACTGT CAGCAGTTCG ATGTGAAAAA 
CAAGCAATTG CTGAGGAATG TCCTTTGCTA ACGTACATCG ATATCCAAGA GTGTATAGAT 
GCCGTAAATC AACAGCGTCA AAATGACGAC GATGTAATAC AAGTTTTACG TCAACTAAAT 
GCGGCTGTTG TAGAGAATGA TCGAGACGGA ATTATAATTG CTTTAAAAGA TACCGCTTTG 
AAGTTGCAGA AGTTTACTTC GCCAGAAGAT GCATCTCTCT ATTTGAAATT ATTTAAAAAA 
TGCCTTACTG AGAAACATTT TGATGGTTCT GAATTATGGT TAGAAGATGT AGAAACGATA 
ACAAAAATCG TTGCTGCGGA ATCTGAAAAT GTCCAAAATG CGTGTACGTT TCTGTCACAA 
GTAAATTTAG GTTTACAACA AAACGATATG TTATTCACAG TTGATTGCCT TCAGACGTTT 
GGTTTTAAAA TATCAGATAA ATACAAAACA GAGTGCTTTC AAAGTTTATG TAACTTACAA 
AAAATGAAGA GTAAAAAGTA CAATTGTGCT CTAGTTCGTT ATATCACTCC TAAAGGCAAC 
GAATCGTACT TGGATTTGAA AAAGTATAAT TATACTTGGG ATTGTCCAAA AAATATATCG 
GAAACATATT ATATCAACAT GGAAGATATC AAGGAAATAA TAAAAAGTAC TACCGCAAGA 
GAACACGAAG AGCATAAAAT CAGCAAACGG ATAGTTCGAT TGCAAGCTTG CATACGAGGA 
TATTTATTAC GAAAAAAAAT TTCTGTTAGA TTTGCATATT TTTATGACAA CGTAGATAAA 
GTTATTAAAA TACAAGCCTG GTGGCGCGGT ATATTACAAC GTAAACGATA CGCCAAATTA 
TTAACAGAAA GAAGAAATTA TAGTAGTGAA AAATTTTCCA GTAACTGTTT AAGAATTAAA 
AATAAGTATG CAAATGTATT AGATTACTAT AAACAACATG AGGAAAAGAT AATAAAGATA 
CAAGCTTTGT GGCGTGGCCG TGCAGAAAGA CGAGCGTTTC ACTCATTGTT GTACATGGAA 
AAACCACCAT TTCCTGTTGT CCGACATTTT TCTGCACTTC TAAATTTTAA TGCAGAAGAT 
TATGACAGAG ATTTACAATT ACAACAATTA AAACACGAAG TGGTACAAAA CATACGGCAT 
AATCAAACTT TATCGCAACA ATTGGATAGT ATGGACATAA AAATTGGTTT GCTTATTCAA 
AATAGGATCA CGCTACAAGA TGTTGTAGCA CATGGGAAGA GTTTAGAAAC ACTTGCGAAG 
CAGAAAAATT CAACTAGAGA TCAGAAGAAT GTTTTATCAG ATAGTACTAT TTCGCATAAA 
GGTTTGAAAT CTTTAACAAA AGAAGGTCGA AAAATGTTAG AGGGGTATCA GCATCTATTC 
TATGCGTTAC AAACTAATCC AACATATTTA TCGAAGATTT TATTTCTTTT GCCGCAAAGC 
AAAACGAATA AGTTTCTTCA AAATGTTATT TTAACGTTAT ATAATTTCGG ATCAAATATA 
AGAGAAGAGT ATTTATTATT GAAACTTTTT GGCAGTGCAT TACAAGAAGA AATTAGGTGT 
AAATTTCAAA AACCGTCTGA AGTCGTAACT GGAAATCCAC TCGTCTTAAA AATGGTAGTG 
AATTACGCAC GACAATTACA TGGTCAAAGA GCTTTACGAC AAATTGTTGG GCCAATCATT 
GAAAAGATAT TAGCCGACCG AACATTGAAT ATAGAAACGA ATCCCGTTGA TATTTATAAG 
TGTTGGAGGA ATCAATTAGA AATGGAGACT GGAGAAACAC TGAATCTTCC ATATACGGTA 
ACGCAACAAC AAGCTTTAAA TTACGAACAG GTACGAATCA GATTAAATAA AGGGATTCAA 
TTGTTGCAAA GTACCGTCTT AGAATTCTTA ACTAAAATAA CAGAATCTCG AGATCTAATA 
CCATACGGAA TGTTATACAT GGCGAAAATT TTAAACGATT CTTTGACTGA AAAATTTCCA 
AACGCTCCGG AAAAAGATAT TCTCAAAGTA GTTGGAAACT TGATTTATTA TCATTTCATT 
AACGCAGCTA TCGTAGCTCC AGATGCTTTC GACATAATTA CCTTACCGAT TGATAGATCA 
TTGTCAAACG ATCAAAGGAG AAATTTAGCC AGCATTGCAA AAATACTACA ATTTGCTGCT 
TCTAAAAAGG GGTTTGGAGA GGAAGCCACA CATTTAGTGT GTCTGAACTC TTTTATAATC 
GAATGCCACG AAAAATTTAA AAAATTCTTT CGTTTTTGTT GCCAAATTGA AGATTTGGAA 
GAACATTTCT GTATTCATGA GTACACCGAG GCCACGCTTA TTCAGAAGCC TGAGATCTAT 
ATTTCCTTAC AAGAAATTTG CGATACTCAC TGCCTCATGT TAGAGTATCA AGACCAAATA 
GCGCCAGATC CAATGGATCC CTTGCATGAT TTGTTAGATG ATTTAGGACC TGCACCTACC 
GTCGCATCTC TGCTAGGAAT CTCCGATACG ATGTGTGAGG CAAATTTAAT ACGGTTTGGA 
AAAACGGAGG TATGTCTGGT ACTTACCAAC AAATTTCAAG TACCCGAAGA TGAAGATGCT 
AGTTTAAACA AACTTTTTAT AAAAACTAAA GAGCTATTGG TTTCTGTACT CCAATTCTTG 
AAAGGACAAA CACTAGTAGA GGCTTTGGAA ACCACTTGTT CTCCTGTTCA AGAGAAACTC 
TATGATGTTA AGTGTTCTAG TTTATCGCCC ACTCTAAGTA TCATTCACAA AAGTTCGTCT 
TTAAATGATT GTAAACTTCA ACTTCGTGCT TACCTTAATA AACTTGAACT TGGAGGATGG 
GTGAGTCGAT CGGATGGATA TCAGACTATT ATAACAGCCG TATCTAAAGA TCTTTGTAAC 
AAAGGAAAGT ACCGAATCAT CCGAAATAAA GAATTACAAA CTTTACGCAT AACTAAACAA 
AGACTGGAAG AAAAGTGCAA ATATTACCAG GAACAGGTAG AATATTATAA CGAATATATA 
CAACGTTGCT TGGAGAACTT GCATACGGGA AAAGGATCTT TACGAGCATT TAAAGCCATT 
CAAAAGAATA ATCATGGCAA GTTACGGTCT AAAATGACAT TGAAATATTC AGCGGCCAAG 
CTACAAGAGA AAGGAATATT ATTAGAAGTG GATGGACTTC CACAATCACA ATTTAAGAAC 
GTAATTTTCG AGATCAGCCC AACAGAGCAT ACCGGATTAT TTAATGTGCG GTGTAAATTT 
ATGGGAGTAG AAATGGAGAA AGTTGATATT GATATACAAA AGCTGCTTGA ACTACAATTT 
GAGGGTTCAC CGATTATGGA CATGTTCGGT AAAGCGAAAG TTAACGTGAA CTTATTACTA 
TATTTACTAA ATCGAAAATT TTATGGTAAA ACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012151733.1
CDS	49..4884
Translation	MDEQRQKTLAYEYLCHLEEAKKWLEACLRETLPQTIELEENLRNGVYLAKLAHFMAPDSLPFNKIYDPEQKRYTAVGLQFRHTDNINYFLKSLKTVQLPLTFQPETTDIYDKKNMPRVIYCIHALSTHLFKLGRAPQIQDLYGKVNFTDDEINAVSKELKKYGIQMPSFQKIGGLLANSMEIDAVTLHAAVIAINQAITEKDNVKMLMALQNKVVQINNINPLYIKEYGDTLTEAKYSKQQAALNRSLNDSYVPDIYDELLTQAEIQGHINYVNVECAVKNISRSIHHNNNEFVNALRASALCLKNIKHENAELYKEQLMQLLESSEWNNMYSESNHHLKQLLQKEIDKSNETALKSRKREEGVKLLNSTLQNGLKNEFSEALKSPKLGLIEQIDDFAIPLYYEEMKIDRTESQNDLSYSDIVVSIRVLSVIAAISKAVDTGNPDLVYQSLENPDCHVSGLDHENKVKYYRALSAVRCEKQAIAEECPLLTYIDIQECIDAVNQQRQNDDDVIQVLRQLNAAVVENDRDGIIIALKDTALKLQKFTSPEDASLYLKLFKKCLTEKHFDGSELWLEDVETITKIVAAESENVQNACTFLSQVNLGLQQNDMLFTVDCLQTFGFKISDKYKTECFQSLCNLQKMKSKKYNCALVRYITPKGNESYLDLKKYNYTWDCPKNISETYYINMEDIKEIIKSTTAREHEEHKISKRIVRLQACIRGYLLRKKISVRFAYFYDNVDKVIKIQAWWRGILQRKRYAKLLTERRNYSSEKFSSNCLRIKNKYANVLDYYKQHEEKIIKIQALWRGRAERRAFHSLLYMEKPPFPVVRHFSALLNFNAEDYDRDLQLQQLKHEVVQNIRHNQTLSQQLDSMDIKIGLLIQNRITLQDVVAHGKSLETLAKQKNSTRDQKNVLSDSTISHKGLKSLTKEGRKMLEGYQHLFYALQTNPTYLSKILFLLPQSKTNKFLQNVILTLYNFGSNIREEYLLLKLFGSALQEEIRCKFQKPSEVVTGNPLVLKMVVNYARQLHGQRALRQIVGPIIEKILADRTLNIETNPVDIYKCWRNQLEMETGETLNLPYTVTQQQALNYEQVRIRLNKGIQLLQSTVLEFLTKITESRDLIPYGMLYMAKILNDSLTEKFPNAPEKDILKVVGNLIYYHFINAAIVAPDAFDIITLPIDRSLSNDQRRNLASIAKILQFAASKKGFGEEATHLVCLNSFIIECHEKFKKFFRFCCQIEDLEEHFCIHEYTEATLIQKPEIYISLQEICDTHCLMLEYQDQIAPDPMDPLHDLLDDLGPAPTVASLLGISDTMCEANLIRFGKTEVCLVLTNKFQVPEDEDASLNKLFIKTKELLVSVLQFLKGQTLVEALETTCSPVQEKLYDVKCSSLSPTLSIIHKSSSLNDCKLQLRAYLNKLELGGWVSRSDGYQTIITAVSKDLCNKGKYRIIRNKELQTLRITKQRLEEKCKYYQEQVEYYNEYIQRCLENLHTGKGSLRAFKAIQKNNHGKLRSKMTLKYSAAKLQEKGILLEVDGLPQSQFKNVIFEISPTEHTGLFNVRCKFMGVEMEKVDIDIQKLLELQFEGSPIMDMFGKAKVNVNLLLYLLNRKFYGKT

Target ORF information:

RefSeq Version	XM_012296343.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata ras GTPase-activating-like protein IQGAP1 (LOC100880131), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012296343.1

ORF Insert Sequence:

ATGGACGAAC AACGTCAAAA AACTCTTGCT TACGAGTACC TTTGTCACTT AGAGGAAGCT 
AAAAAATGGC TGGAAGCCTG TCTGAGAGAA ACTCTCCCAC AAACTATTGA ATTAGAAGAA 
AACTTAAGGA ACGGGGTATA TTTAGCAAAA CTTGCACATT TTATGGCACC TGATTCTTTA 
CCCTTTAATA AAATTTATGA CCCTGAACAG AAGCGTTATA CTGCGGTTGG TTTACAGTTT 
CGTCATACAG ATAATATTAA TTATTTTTTA AAATCCTTAA AAACTGTGCA ATTACCATTG 
ACTTTCCAAC CAGAAACTAC AGACATCTAT GATAAAAAAA ATATGCCACG CGTTATATAT 
TGCATTCATG CTCTTAGTAC TCATTTATTC AAACTTGGTA GAGCTCCACA GATTCAGGAT 
TTGTATGGAA AAGTGAACTT CACTGACGAC GAAATAAATG CTGTTAGTAA AGAATTGAAG 
AAGTATGGAA TTCAAATGCC TTCGTTCCAA AAAATTGGCG GATTATTAGC GAACAGTATG 
GAGATCGATG CTGTCACGCT TCATGCTGCG GTAATAGCAA TTAACCAGGC CATCACAGAA 
AAAGATAATG TTAAAATGTT AATGGCGCTT CAGAATAAAG TAGTGCAAAT AAATAATATT 
AACCCTCTCT ACATTAAGGA ATATGGCGAT ACATTAACAG AGGCAAAGTA TTCTAAGCAA 
CAGGCTGCTC TTAATAGATC TCTTAACGAT AGTTATGTGC CAGATATTTA TGATGAATTG 
TTAACTCAAG CAGAAATTCA GGGACATATT AATTATGTTA ATGTCGAGTG CGCAGTGAAA 
AACATAAGTC GGTCAATTCA TCATAACAAT AACGAGTTCG TGAATGCACT GAGAGCATCT 
GCTTTGTGTT TAAAAAACAT TAAGCATGAA AATGCTGAAT TATATAAAGA ACAGTTAATG 
CAGTTACTAG AAAGTTCTGA ATGGAATAAT ATGTATTCGG AAAGCAATCA TCATTTGAAA 
CAATTGCTTC AGAAAGAAAT TGATAAATCA AATGAAACAG CGCTTAAATC TCGGAAACGT 
GAAGAAGGCG TTAAACTCTT AAACTCAACT TTACAAAATG GTTTGAAGAA TGAGTTCTCT 
GAGGCCTTAA AAAGTCCGAA ATTGGGATTG ATCGAACAAA TCGATGATTT TGCAATACCA 
TTATATTACG AAGAAATGAA AATCGATCGT ACGGAATCTC AGAACGACCT TTCATATAGT 
GATATTGTAG TGAGTATTCG TGTATTGTCA GTGATCGCAG CGATCAGTAA AGCTGTAGAT 
ACGGGAAATC CAGATTTAGT TTATCAGTCA TTAGAAAATC CAGACTGTCA TGTTTCTGGT 
TTGGATCATG AAAACAAAGT GAAGTATTAT AGAGCACTGT CAGCAGTTCG ATGTGAAAAA 
CAAGCAATTG CTGAGGAATG TCCTTTGCTA ACGTACATCG ATATCCAAGA GTGTATAGAT 
GCCGTAAATC AACAGCGTCA AAATGACGAC GATGTAATAC AAGTTTTACG TCAACTAAAT 
GCGGCTGTTG TAGAGAATGA TCGAGACGGA ATTATAATTG CTTTAAAAGA TACCGCTTTG 
AAGTTGCAGA AGTTTACTTC GCCAGAAGAT GCATCTCTCT ATTTGAAATT ATTTAAAAAA 
TGCCTTACTG AGAAACATTT TGATGGTTCT GAATTATGGT TAGAAGATGT AGAAACGATA 
ACAAAAATCG TTGCTGCGGA ATCTGAAAAT GTCCAAAATG CGTGTACGTT TCTGTCACAA 
GTAAATTTAG GTTTACAACA AAACGATATG TTATTCACAG TTGATTGCCT TCAGACGTTT 
GGTTTTAAAA TATCAGATAA ATACAAAACA GAGTGCTTTC AAAGTTTATG TAACTTACAA 
AAAATGAAGA GTAAAAAGTA CAATTGTGCT CTAGTTCGTT ATATCACTCC TAAAGGCAAC 
GAATCGTACT TGGATTTGAA AAAGTATAAT TATACTTGGG ATTGTCCAAA AAATATATCG 
GAAACATATT ATATCAACAT GGAAGATATC AAGGAAATAA TAAAAAGTAC TACCGCAAGA 
GAACACGAAG AGCATAAAAT CAGCAAACGG ATAGTTCGAT TGCAAGCTTG CATACGAGGA 
TATTTATTAC GAAAAAAAAT TTCTGTTAGA TTTGCATATT TTTATGACAA CGTAGATAAA 
GTTATTAAAA TACAAGCCTG GTGGCGCGGT ATATTACAAC GTAAACGATA CGCCAAATTA 
TTAACAGAAA GAAGAAATTA TAGTAGTGAA AAATTTTCCA GTAACTGTTT AAGAATTAAA 
AATAAGTATG CAAATGTATT AGATTACTAT AAACAACATG AGGAAAAGAT AATAAAGATA 
CAAGCTTTGT GGCGTGGCCG TGCAGAAAGA CGAGCGTTTC ACTCATTGTT GTACATGGAA 
AAACCACCAT TTCCTGTTGT CCGACATTTT TCTGCACTTC TAAATTTTAA TGCAGAAGAT 
TATGACAGAG ATTTACAATT ACAACAATTA AAACACGAAG TGGTACAAAA CATACGGCAT 
AATCAAACTT TATCGCAACA ATTGGATAGT ATGGACATAA AAATTGGTTT GCTTATTCAA 
AATAGGATCA CGCTACAAGA TGTTGTAGCA CATGGGAAGA GTTTAGAAAC ACTTGCGAAG 
CAGAAAAATT CAACTAGAGA TCAGAAGAAT GTTTTATCAG ATAGTACTAT TTCGCATAAA 
GGTTTGAAAT CTTTAACAAA AGAAGGTCGA AAAATGTTAG AGGGGTATCA GCATCTATTC 
TATGCGTTAC AAACTAATCC AACATATTTA TCGAAGATTT TATTTCTTTT GCCGCAAAGC 
AAAACGAATA AGTTTCTTCA AAATGTTATT TTAACGTTAT ATAATTTCGG ATCAAATATA 
AGAGAAGAGT ATTTATTATT GAAACTTTTT GGCAGTGCAT TACAAGAAGA AATTAGGTGT 
AAATTTCAAA AACCGTCTGA AGTCGTAACT GGAAATCCAC TCGTCTTAAA AATGGTAGTG 
AATTACGCAC GACAATTACA TGGTCAAAGA GCTTTACGAC AAATTGTTGG GCCAATCATT 
GAAAAGATAT TAGCCGACCG AACATTGAAT ATAGAAACGA ATCCCGTTGA TATTTATAAG 
TGTTGGAGGA ATCAATTAGA AATGGAGACT GGAGAAACAC TGAATCTTCC ATATACGGTA 
ACGCAACAAC AAGCTTTAAA TTACGAACAG GTACGAATCA GATTAAATAA AGGGATTCAA 
TTGTTGCAAA GTACCGTCTT AGAATTCTTA ACTAAAATAA CAGAATCTCG AGATCTAATA 
CCATACGGAA TGTTATACAT GGCGAAAATT TTAAACGATT CTTTGACTGA AAAATTTCCA 
AACGCTCCGG AAAAAGATAT TCTCAAAGTA GTTGGAAACT TGATTTATTA TCATTTCATT 
AACGCAGCTA TCGTAGCTCC AGATGCTTTC GACATAATTA CCTTACCGAT TGATAGATCA 
TTGTCAAACG ATCAAAGGAG AAATTTAGCC AGCATTGCAA AAATACTACA ATTTGCTGCT 
TCTAAAAAGG GGTTTGGAGA GGAAGCCACA CATTTAGTGT GTCTGAACTC TTTTATAATC 
GAATGCCACG AAAAATTTAA AAAATTCTTT CGTTTTTGTT GCCAAATTGA AGATTTGGAA 
GAACATTTCT GTATTCATGA GTACACCGAG GCCACGCTTA TTCAGAAGCC TGAGATCTAT 
ATTTCCTTAC AAGAAATTTG CGATACTCAC TGCCTCATGT TAGAGTATCA AGACCAAATA 
GCGCCAGATC CAATGGATCC CTTGCATGAT TTGTTAGATG ATTTAGGACC TGCACCTACC 
GTCGCATCTC TGCTAGGAAT CTCCGATACG ATGTGTGAGG CAAATTTAAT ACGGTTTGGA 
AAAACGGAGG TATGTCTGGT ACTTACCAAC AAATTTCAAG TACCCGAAGA TGAAGATGCT 
AGTTTAAACA AACTTTTTAT AAAAACTAAA GAGCTATTGG TTTCTGTACT CCAATTCTTG 
AAAGGACAAA CACTAGTAGA GGCTTTGGAA ACCACTTGTT CTCCTGTTCA AGAGAAACTC 
TATGATGTTA AGTGTTCTAG TTTATCGCCC ACTCTAAGTA TCATTCACAA AAGTTCGTCT 
TTAAATGATT GTAAACTTCA ACTTCGTGCT TACCTTAATA AACTTGAACT TGGAGGATGG 
GTGAGTCGAT CGGATGGATA TCAGACTATT ATAACAGCCG TATCTAAAGA TCTTTGTAAC 
AAAGGAAAGT ACCGAATCAT CCGAAATAAA GAATTACAAA CTTTACGCAT AACTAAACAA 
AGACTGGAAG AAAAGTGCAA ATATTACCAG GAACAGGTAG AATATTATAA CGAATATATA 
CAACGTTGCT TGGAGAACTT GCATACGGGA AAAGGATCTT TACGAGCATT TAAAGCCATT 
CAAAAGAATA ATCATGGCAA GTTACGGTCT AAAATGACAT TGAAATATTC AGCGGCCAAG 
CTACAAGAGA AAGGAATATT ATTAGAAGTG GATGGACTTC CACAATCACA ATTTAAGAAC 
GTAATTTTCG AGATCAGCCC AACAGAGCAT ACCGGATTAT TTAATGTGCG GTGTAAATTT 
ATGGGAGTAG AAATGGAGAA AGTTGATATT GATATACAAA AGCTGCTTGA ACTACAATTT 
GAGGGTTCAC CGATTATGGA CATGTTCGGT AAAGCGAAAG TTAACGTGAA CTTATTACTA 
TATTTACTAA ATCGAAAATT TTATGGTAAA ACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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[email protected]

電話

03-6811-6572

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03-6811-6573
050-3174-7589(受注専用)

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オンライン注文

LOC100880131 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム