LOC100879158 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100879158
Entrez Gene ID	100879158
Full Name	polycomb protein Scm
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003706403.2	XP_003706451.1	polycomb protein Scm

Pathways from BioSystems
Gene Ontology

The following LOC100879158 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100879158 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe111092	XM_003706403.2 Latest version!	Megachile rotundata polycomb protein Scm (LOC100879158), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100879158 ( XM_003706403.2 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_003706451.1 Megachile rotundata polycomb protein Scm (LOC100879158), mRNA.

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CloneID	OMe111092
Clone ID Related Accession (Same CDS sequence)	XM_003706403.2
Accession Version	XM_003706403.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	polycomb protein Scm
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797424.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2015 this sequence version replaced XM_003706403.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTCTA CACAAAGCAA AATGCGAGGT CCAGGAAGAC CACCAAAATC AAAAAATTCC 
TGTACATGGT GTGGCGAAAC TAAACAGCCT TTAAAGTATG TCCTTCCAAC GCAGCATGGA 
AAAAAGGAAT TTTGTTCAGA AACATGTTTG TCTGAATTTC GCAAAGCTTA TGTACGAGGA 
GCTTGCGTTC AATGTGACAA CGTTATCAGA GGCACACCAG TGAGATTAGA ACAGAAGGAT 
GGACCCACAA AAGACTTTTG TTCTTCGTTT TGTTTGAATA AACATCAGAA GAGAGAAGGG 
CAAGTGGATA TGAAGAAAAA TAATAGGGAC CATTCATCAC CTGCGCCATC TCTTACGCCA 
TCCGGATTAA TAAGTGGAAA TAATATTCCA TCTACAACTC AATCATTTAC AAGTACAAAT 
CACACAAATG TATCTCATCC TCCGTCAACT TCTACCGGCC CATTTCAATA TGAAACATAC 
CAAACTTTTG ATTGGGATCT GTATTTGAAA GAAACAAACA GTCAAGCAGC ACCAATTGAA 
TGTTTTAAAC AGCATGAAGT TCCACCAACT AACGAGTTTA AAATGGGTAT GAAGTTAGAA 
GCATTAGACC CTCGTAATTT AACATCAACA TGTATTGCAA CTGTAGTTGG TGTCCTTGGT 
CCTCGTTTAA GATTAAGGTT GGATGGCTCA GACAACAAAA ATGATTTCTG GCGTTTGGTT 
GACAGTAATG AAATTCATCC AATTGGACAT TGTGAAAAAT CTGGTGGAAT GTTGCAACCT 
CCATTAGGCT TCCGAATGAA TGCTTCTAGT TGGCCAATGT TTCTTTTGAA AACATTGAAT 
GGTGCAGAAA TGGCCCCTGC AAAAGTGTTC AAACGTGAAC CAAAAACACC ACGTTCTAAC 
TTGTTTGAAG TTGGACATAA ATTAGAAGCC ATAGATAAAA AGAATCCGCA ACTTATATGT 
ACGGCGACTG TTGGTGCTGT TAAAGATGAT ATGATTCACA TTACATTTGA TGGATGGAGA 
GGAGCATTTG ATTATTGGTG TCGTTTTGAC TCCAGAGATA TTTTTCCTGC CGGTTGGTGC 
TTTAAAAGTG GTCATCCATT ACAACCACCA AGACAGAAAT CAACTGGGCC TAATAGATTT 
AAGTCAAGAA CTAGTAATAT CTTACCAGTA ATGGCAGTAT CTGGTGCGGG CACTAATGGA 
GAACCGGCCG TTGCATTGGT GAGTCCTGCT GGTTCCAGTG CTCCACCGCA ACCAGCCACA 
GAACCCGATA CTAGTACAGC TAATACCAAA TCAGATACTA TAGAGAATAG TATGCAGTTG 
GATAATTCTA CACTATCATC ACCAACAGCT GCAAAGCAAC CACGAAAATC CGTTCCAGAA 
CTTGAAGCAG CAACTTCTAC TACCCAATCT GAAACTACCA CAAACCGTAC ACCTTCTGCA 
GAACCAGCAG AATGGACAAT TGAAGATGTA ATACATTACA TCGGTGTTAC CGATCCTGCA 
TTAGGACAGC ATGCAGATTT ATTTAGAAAA CATGAAATTG ACGGAAAAGC TTTATTACTT 
TTGAATTCTG ACATGATGAT GAAATACATG GGACTGAAAC TTGGGCCAGC GCTTAAAATA 
TGCAACTTGG TAAATCGTAT TAAAGGCAGA AGGCATATAC TTCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003706451.1
CDS	181..1848
Translation	MSSTQSKMRGPGRPPKSKNSCTWCGETKQPLKYVLPTQHGKKEFCSETCLSEFRKAYVRGACVQCDNVIRGTPVRLEQKDGPTKDFCSSFCLNKHQKREGQVDMKKNNRDHSSPAPSLTPSGLISGNNIPSTTQSFTSTNHTNVSHPPSTSTGPFQYETYQTFDWDLYLKETNSQAAPIECFKQHEVPPTNEFKMGMKLEALDPRNLTSTCIATVVGVLGPRLRLRLDGSDNKNDFWRLVDSNEIHPIGHCEKSGGMLQPPLGFRMNASSWPMFLLKTLNGAEMAPAKVFKREPKTPRSNLFEVGHKLEAIDKKNPQLICTATVGAVKDDMIHITFDGWRGAFDYWCRFDSRDIFPAGWCFKSGHPLQPPRQKSTGPNRFKSRTSNILPVMAVSGAGTNGEPAVALVSPAGSSAPPQPATEPDTSTANTKSDTIENSMQLDNSTLSSPTAAKQPRKSVPELEAATSTTQSETTTNRTPSAEPAEWTIEDVIHYIGVTDPALGQHADLFRKHEIDGKALLLLNSDMMMKYMGLKLGPALKICNLVNRIKGRRHILL

Target ORF information:

RefSeq Version	XM_003706403.2
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata polycomb protein Scm (LOC100879158), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003706403.2

ORF Insert Sequence:

ATGTCGTCTA CACAAAGCAA AATGCGAGGT CCAGGAAGAC CACCAAAATC AAAAAATTCC 
TGTACATGGT GTGGCGAAAC TAAACAGCCT TTAAAGTATG TCCTTCCAAC GCAGCATGGA 
AAAAAGGAAT TTTGTTCAGA AACATGTTTG TCTGAATTTC GCAAAGCTTA TGTACGAGGA 
GCTTGCGTTC AATGTGACAA CGTTATCAGA GGCACACCAG TGAGATTAGA ACAGAAGGAT 
GGACCCACAA AAGACTTTTG TTCTTCGTTT TGTTTGAATA AACATCAGAA GAGAGAAGGG 
CAAGTGGATA TGAAGAAAAA TAATAGGGAC CATTCATCAC CTGCGCCATC TCTTACGCCA 
TCCGGATTAA TAAGTGGAAA TAATATTCCA TCTACAACTC AATCATTTAC AAGTACAAAT 
CACACAAATG TATCTCATCC TCCGTCAACT TCTACCGGCC CATTTCAATA TGAAACATAC 
CAAACTTTTG ATTGGGATCT GTATTTGAAA GAAACAAACA GTCAAGCAGC ACCAATTGAA 
TGTTTTAAAC AGCATGAAGT TCCACCAACT AACGAGTTTA AAATGGGTAT GAAGTTAGAA 
GCATTAGACC CTCGTAATTT AACATCAACA TGTATTGCAA CTGTAGTTGG TGTCCTTGGT 
CCTCGTTTAA GATTAAGGTT GGATGGCTCA GACAACAAAA ATGATTTCTG GCGTTTGGTT 
GACAGTAATG AAATTCATCC AATTGGACAT TGTGAAAAAT CTGGTGGAAT GTTGCAACCT 
CCATTAGGCT TCCGAATGAA TGCTTCTAGT TGGCCAATGT TTCTTTTGAA AACATTGAAT 
GGTGCAGAAA TGGCCCCTGC AAAAGTGTTC AAACGTGAAC CAAAAACACC ACGTTCTAAC 
TTGTTTGAAG TTGGACATAA ATTAGAAGCC ATAGATAAAA AGAATCCGCA ACTTATATGT 
ACGGCGACTG TTGGTGCTGT TAAAGATGAT ATGATTCACA TTACATTTGA TGGATGGAGA 
GGAGCATTTG ATTATTGGTG TCGTTTTGAC TCCAGAGATA TTTTTCCTGC CGGTTGGTGC 
TTTAAAAGTG GTCATCCATT ACAACCACCA AGACAGAAAT CAACTGGGCC TAATAGATTT 
AAGTCAAGAA CTAGTAATAT CTTACCAGTA ATGGCAGTAT CTGGTGCGGG CACTAATGGA 
GAACCGGCCG TTGCATTGGT GAGTCCTGCT GGTTCCAGTG CTCCACCGCA ACCAGCCACA 
GAACCCGATA CTAGTACAGC TAATACCAAA TCAGATACTA TAGAGAATAG TATGCAGTTG 
GATAATTCTA CACTATCATC ACCAACAGCT GCAAAGCAAC CACGAAAATC CGTTCCAGAA 
CTTGAAGCAG CAACTTCTAC TACCCAATCT GAAACTACCA CAAACCGTAC ACCTTCTGCA 
GAACCAGCAG AATGGACAAT TGAAGATGTA ATACATTACA TCGGTGTTAC CGATCCTGCA 
TTAGGACAGC ATGCAGATTT ATTTAGAAAA CATGAAATTG ACGGAAAAGC TTTATTACTT 
TTGAATTCTG ACATGATGAT GAAATACATG GGACTGAAAC TTGGGCCAGC GCTTAAAATA 
TGCAACTTGG TAAATCGTAT TAAAGGCAGA AGGCATATAC TTCTATGA