LOC100878927 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100878927
Entrez Gene ID	100878927
Full Name	citron Rho-interacting kinase-like
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012285728.1	XP_012141118.1	citron Rho-interacting kinase-like isoform X1
XM_012285729.1	XP_012141119.1	citron Rho-interacting kinase-like isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC100878927 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100878927 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe105025	XM_012285729.1 Latest version!	Megachile rotundata citron Rho-interacting kinase-like (LOC100878927), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OMe105024	XM_012285728.1 Latest version!	Megachile rotundata citron Rho-interacting kinase-like (LOC100878927), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100878927 ( XM_012285729.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012141119.1 Megachile rotundata citron Rho-interacting kinase-like (LOC100878927), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMe105025
Clone ID Related Accession (Same CDS sequence)	XM_012285729.1
Accession Version	XM_012285729.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	citron Rho-interacting kinase-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797177.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTTG AAGACAGAGG GAAAGAATTT CAGGAGCGTT ATAAAAAGGT TTGTGGAAAA 
AGATTGTCTT CCGAATATGA GACTCAAACG AAGGAAAGCA CAAGAAAGAC GGAAACCTCC 
ATGAAAGAAA AAGAAAAGAT GGAAGAAGAA TTAAATGCAG CAAAATGTGA TTTAGCAAGT 
CTTCGAAAAA GTCTAAGTGG TGATAGAAAC ATTCCTGCAT CAGAATCTGA TGATCTTTCC 
AAAGATGTCC CAAAAGAATG TTCGTCTTTC TTTGAGTTTA AATTTCACAA ACATAATAAT 
GCGCAGTATC AAAAATATTC CAAAGAAAAG AGCGAATTGG AAGCAGAGTT AGATCTTAAA 
AAGAAAGAAT TACAGGAGAA GAAACAGGAA ATATTTGAAT TAAAGGAAAG AGTAAAAAGA 
TTAGAAGCAC TTAGTAAGGA AAGAGATATA CTTGAAAAGG AATTAATTAC CACGAAATCA 
GAATTAACTG GGATAAAAAG AACATTAGAA CTAGAACGGC AAGAGAGAAG AGATTTGGAG 
ACTAGAGCTC TCAGTTTGAT TAAAGATGCT AAGCGTAAAT GGGAAAATGC GGAAAAAGAT 
AAAATTGCAC AACTGAATAA ACATATTGAA ATGCAAACTG TAAGAATTAC AGAGTTATGT 
ACTAGTAATA ATGAAATGAG TTCAAGATTA CAAAGGACTG AATGTGAACT CGAAACTGCA 
AATGCAGAAT TGCATAAACT TCGAGTGTTT CAGATGCAAT ATAAAGAGTC GCTAGTTAAA 
ACTAGAGAAT TAAACCGGCA AAGTGCACAA GGAGTTGAAA CTAAATTGAA GGAAATAGCT 
GCAAGAGCAC ATAATCAGTT AGCTGAACTT AGATCGAAAT TAGATTTAGA AACAGCAAAA 
AATACTGACT TGGAAACTAA ACTACGAAAT GAACAAGATT CTAATCATTG CCGTCAATCA 
CGGTTAAATG TAGCTTTAGA GTTTGCACAA AATGAATTAA AGGACTGTCA GGAACAGTTA 
CGTACAATAC AAGCTACAAT ACCTGCCAGA GATGCTGAAA TAGACGTTTT AAAAAAACAA 
TTACAAGAAA GATCAAAACA ATTAGATAGT GCTATAGCAT CAGAACAGAT AGTAGCTACA 
ATGCAAGAGC AATTGGAGCG ATCAAGACTT GAGAATGAAC AATTGAAACA ACAGTTAGAG 
GTAATGAAGT CTGACTTAAG TGAAACAATG ATGAATTTAG AACAAAATGA AAACCTTGCT 
TTAAATTTAG AACAAGCTAC ACAAGATAAA GTTGCGCTCC AAAAACGACT ACAAGATTCT 
CTCGATAAAG AAGAAGAACA TTTGCGAAAA GTCGGTAATT TAGAAGAATT ATTAAGACGC 
TTAGAGCAAA GCGTTACTAA ACTCGAAACA GAAAATGCTA ATCTCAAAAT GGAAAATATA 
GAACCTAGTA TTGGCATGGT GCCAACAAGT GGTGCGATAA AACGTGATAT TCTGTCAGAG 
GAACACATAC AAAAATTAGA ACAGGAACTT CGAACGACAA AAGAGGATTT AAATAAAGAA 
CGTCAAGCCG TGAAACAAGC ACAAATTAAT TTATGGAAGA AAGAAAAAGA ATTATCGGAT 
GCAAATTTAG ATAAGAGAAT TGCAATAAGG TCAAATAAAA AGGCAGAGGA GAAAGTTAAA 
ATATTGCAAG AAGAAAAACA AAAATTATCA ATGAAATTAG ATGACAAAAT AAAAGAAGAA 
GAAGAGAAAT CCAAAAAATT GCTTAAAGAA TTAGACAGCG CAAAAGCATC ATTAAACGAT 
ATAACAAAAG AATCTTCTAG AAACAGAATG CAAGCTGATT CTGCTCAACG AGCGCTAACT 
CAAGCTAATC ATCAGATAGA AGAACTTCAA TCTTCAAGTG CTTTGCTGCG CAGAGAATTA 
GACGCTGCTC GAAAACAAGT AAGGGTAAGT CAAGATAGAC TGGAAAGTTT AAATGCTGAG 
AATAAGCGTT TGTCACAATC AATTACAAAA CATAACGAAG AAAAGAGTGC ATTGGAATCT 
AGAATAGAAA AGTTAGAACA AGAAATCAAA GGTTACGAAT TAAATACTGA ACTTCTAAAA 
GAAACATGTA CAGTTCTTGA AGAGCAACTT ACTGATTACG AAAGATTAAC TAGCGATCAT 
GAAACGCGAG AAAATATATT GATTCAAGAT AAAATGAAAT TGCAAAAAGA TTTAGAAGCA 
ACTGAAGCTA AACTTCGCGA AGCACGTGCT GCGCAAAATG AAGAAAAATC TATGAGATTA 
GCTGCGGAAC GTAACATCGA AAGACTTGAA TCGGAGACGA GCGATATAGA AAGTGAAAAG 
AACGGTTTAC TTGCTCAAAG AGATCAGTAT AGAAAGCTTG TTCAAGAATT GAGTGCACAG 
GTTGATGAAT TATCAAGTAA GTGCGGTGAA TTGGAATGTG ATCTTTCTGA AATGAGACGT 
AATTTAGAAG TGACCAAAGC TGAAACGAGA GTTGTCAAGG AAGAAAGTAG CCAACACTTA 
ACAAGAGTGC ACGAATTGAA AGAAGCAAAC ACCATGCTTA TGAACGACTT GCAGAGTAGC 
GTAGATCAGG GACAGGAGCT TCGAATGAGA ATTTCCGAAT TGGAAAGCAT CTTGGAAGAA 
ATGCGGCAAT TCTATCAAGA AAGAGAAGTA AAAGCTGAAA GTACAAGGCA ACAGCAGACA 
AAACTGATCG ATTATTTGCA GTTCAAATTA GAAGAATGTA GCAAAAAGAA GAAAACAGTA 
TGCAATAAAA TACTTGGTAC TAAACAAAAA GAAAATGTTC CGCCCTGTGG TACTGGTATG 
CCAGTTGGGT ATCGCGAATT AGAGAATCAG TTAACTAAAG AACGTGCTAA AGTTAAGACG 
TTAACTGATC AATTATTAGC GCTTAAAGCT ATGCATGCTT CAACCTCTGC CCCTACGTCT 
CCTTCAGCAC AGAGTATCAA GAATACAAAT TATATTTCTG AACAATCGGT CGACTCGTTA 
TCCAAACGAT TATCGCCCCA AAGAATTGGA CATAATATTC CACATCGTTT CGATGTCAGT 
TTACCTATGA GGGCTGGGAA ATGCTCCGCG TGTTTAGATC CTATTCAGTT CGGTAAACGT 
GCGGCTATTT GCAGTGAATG CGCAGTAATG ACGCACTTAA AATGTGCTGT TACTGTTCCC 
GCCACTTGCG GTTTGCCGGG CGGTTTCGCT AAACAATTCG GCAAAAGTTG GAGGAATAGC 
AATGACAGCT TGTCATCTTT ATCTGGAAGT GTTCAAACGT TAAATATAGA TCAACCGGAT 
AAACCTGATA CGGATATATG CAACGCACAA GATAAAGGTA ATAATGTATC AATGGAAAGT 
TGGGTCAAAG TTCCAGAGCG GTCAAAAAGC TGTTGGGAAA GAAAGTACCT TAGATTAGAA 
GGATCATGTT TATGTACTTA CGAGCACCAA CCATCTTCTG GGATGGCACC CATAAATCGT 
TTAGATTTGG TCGAAAAAGA TGGATTTAAT GTACTAGAAA ACGTACATCA ACCCGATGTA 
CAAGGAACAG CAAAGTCGGA TATACCATTT ATCTTTAGGA TAGAATCTAA TTCTTCCACG 
ACCTGTTGGC CTACATCGCG TTTAGATGTA ATGACTTTAA GTCAGACGGA TAAGAAAAAT 
TGGTTGAAAG CTTTGAAGTC TATTACTAGT CAAAATTATT CGAGTAACGC TTATAAAAGT 
AAAAAATATG AAATTGTATT AAGACTAGAA AAGAATCAGT TGGATTTGAA TTGTGCTGTA 
CATCTACCCG AAGATAATGT GTTGTTATTA GGCGCCGAAG AAGGACTCTT TTCATATTGC 
GGTGCTAAAT CACGAATGCT TACAGCGATT CGTGGAGTAA AGAGGGTTCA CCAATTAACG 
TTGCATCCTC ATTTGGGAAT AGCTTTAATG ATAGCCGGCG AAAATAGGCA ATTGGTATCT 
TGTAATCTGA GACAATTGAA GAGTAACGCA GTAGCTGCTG AGTGTTCGAG GCCAGCAATT 
AATACAAACC CAGTTTTAAC TGGCACTGAT AGTTGTCATT TATATCAGTT ACAAAGAGAT 
ATGCTTTGCG CCGCTACAGC AACTCACGTC ATATTACTTA AATGGTACAC CGAGGAAGAA 
GGAGGAGAAT TTGTTGGTGT ACGCGAACTT GAAACATCTG AGCCATGCAG CTGTGCCATA 
TTTACTCAAA ATGTTCTGGT AGTAGGATGC AACAAGTTCT TCCAAATCGA TCTGAGAAGC 
TATTGTGTCG ATGGATACAA GATGACGAAA GCAGTTCAGA GTTCAGTTTT ACATCAAGCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012141119.1
CDS	505..4827
Translation	MDFEDRGKEFQERYKKVCGKRLSSEYETQTKESTRKTETSMKEKEKMEEELNAAKCDLASLRKSLSGDRNIPASESDDLSKDVPKECSSFFEFKFHKHNNAQYQKYSKEKSELEAELDLKKKELQEKKQEIFELKERVKRLEALSKERDILEKELITTKSELTGIKRTLELERQERRDLETRALSLIKDAKRKWENAEKDKIAQLNKHIEMQTVRITELCTSNNEMSSRLQRTECELETANAELHKLRVFQMQYKESLVKTRELNRQSAQGVETKLKEIAARAHNQLAELRSKLDLETAKNTDLETKLRNEQDSNHCRQSRLNVALEFAQNELKDCQEQLRTIQATIPARDAEIDVLKKQLQERSKQLDSAIASEQIVATMQEQLERSRLENEQLKQQLEVMKSDLSETMMNLEQNENLALNLEQATQDKVALQKRLQDSLDKEEEHLRKVGNLEELLRRLEQSVTKLETENANLKMENIEPSIGMVPTSGAIKRDILSEEHIQKLEQELRTTKEDLNKERQAVKQAQINLWKKEKELSDANLDKRIAIRSNKKAEEKVKILQEEKQKLSMKLDDKIKEEEEKSKKLLKELDSAKASLNDITKESSRNRMQADSAQRALTQANHQIEELQSSSALLRRELDAARKQVRVSQDRLESLNAENKRLSQSITKHNEEKSALESRIEKLEQEIKGYELNTELLKETCTVLEEQLTDYERLTSDHETRENILIQDKMKLQKDLEATEAKLREARAAQNEEKSMRLAAERNIERLESETSDIESEKNGLLAQRDQYRKLVQELSAQVDELSSKCGELECDLSEMRRNLEVTKAETRVVKEESSQHLTRVHELKEANTMLMNDLQSSVDQGQELRMRISELESILEEMRQFYQEREVKAESTRQQQTKLIDYLQFKLEECSKKKKTVCNKILGTKQKENVPPCGTGMPVGYRELENQLTKERAKVKTLTDQLLALKAMHASTSAPTSPSAQSIKNTNYISEQSVDSLSKRLSPQRIGHNIPHRFDVSLPMRAGKCSACLDPIQFGKRAAICSECAVMTHLKCAVTVPATCGLPGGFAKQFGKSWRNSNDSLSSLSGSVQTLNIDQPDKPDTDICNAQDKGNNVSMESWVKVPERSKSCWERKYLRLEGSCLCTYEHQPSSGMAPINRLDLVEKDGFNVLENVHQPDVQGTAKSDIPFIFRIESNSSTTCWPTSRLDVMTLSQTDKKNWLKALKSITSQNYSSNAYKSKKYEIVLRLEKNQLDLNCAVHLPEDNVLLLGAEEGLFSYCGAKSRMLTAIRGVKRVHQLTLHPHLGIALMIAGENRQLVSCNLRQLKSNAVAAECSRPAINTNPVLTGTDSCHLYQLQRDMLCAATATHVILLKWYTEEEGGEFVGVRELETSEPCSCAIFTQNVLVVGCNKFFQIDLRSYCVDGYKMTKAVQSSVLHQA

Target ORF information:

RefSeq Version	XM_012285729.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata citron Rho-interacting kinase-like (LOC100878927), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012285729.1

ORF Insert Sequence:

ATGGATTTTG AAGACAGAGG GAAAGAATTT CAGGAGCGTT ATAAAAAGGT TTGTGGAAAA 
AGATTGTCTT CCGAATATGA GACTCAAACG AAGGAAAGCA CAAGAAAGAC GGAAACCTCC 
ATGAAAGAAA AAGAAAAGAT GGAAGAAGAA TTAAATGCAG CAAAATGTGA TTTAGCAAGT 
CTTCGAAAAA GTCTAAGTGG TGATAGAAAC ATTCCTGCAT CAGAATCTGA TGATCTTTCC 
AAAGATGTCC CAAAAGAATG TTCGTCTTTC TTTGAGTTTA AATTTCACAA ACATAATAAT 
GCGCAGTATC AAAAATATTC CAAAGAAAAG AGCGAATTGG AAGCAGAGTT AGATCTTAAA 
AAGAAAGAAT TACAGGAGAA GAAACAGGAA ATATTTGAAT TAAAGGAAAG AGTAAAAAGA 
TTAGAAGCAC TTAGTAAGGA AAGAGATATA CTTGAAAAGG AATTAATTAC CACGAAATCA 
GAATTAACTG GGATAAAAAG AACATTAGAA CTAGAACGGC AAGAGAGAAG AGATTTGGAG 
ACTAGAGCTC TCAGTTTGAT TAAAGATGCT AAGCGTAAAT GGGAAAATGC GGAAAAAGAT 
AAAATTGCAC AACTGAATAA ACATATTGAA ATGCAAACTG TAAGAATTAC AGAGTTATGT 
ACTAGTAATA ATGAAATGAG TTCAAGATTA CAAAGGACTG AATGTGAACT CGAAACTGCA 
AATGCAGAAT TGCATAAACT TCGAGTGTTT CAGATGCAAT ATAAAGAGTC GCTAGTTAAA 
ACTAGAGAAT TAAACCGGCA AAGTGCACAA GGAGTTGAAA CTAAATTGAA GGAAATAGCT 
GCAAGAGCAC ATAATCAGTT AGCTGAACTT AGATCGAAAT TAGATTTAGA AACAGCAAAA 
AATACTGACT TGGAAACTAA ACTACGAAAT GAACAAGATT CTAATCATTG CCGTCAATCA 
CGGTTAAATG TAGCTTTAGA GTTTGCACAA AATGAATTAA AGGACTGTCA GGAACAGTTA 
CGTACAATAC AAGCTACAAT ACCTGCCAGA GATGCTGAAA TAGACGTTTT AAAAAAACAA 
TTACAAGAAA GATCAAAACA ATTAGATAGT GCTATAGCAT CAGAACAGAT AGTAGCTACA 
ATGCAAGAGC AATTGGAGCG ATCAAGACTT GAGAATGAAC AATTGAAACA ACAGTTAGAG 
GTAATGAAGT CTGACTTAAG TGAAACAATG ATGAATTTAG AACAAAATGA AAACCTTGCT 
TTAAATTTAG AACAAGCTAC ACAAGATAAA GTTGCGCTCC AAAAACGACT ACAAGATTCT 
CTCGATAAAG AAGAAGAACA TTTGCGAAAA GTCGGTAATT TAGAAGAATT ATTAAGACGC 
TTAGAGCAAA GCGTTACTAA ACTCGAAACA GAAAATGCTA ATCTCAAAAT GGAAAATATA 
GAACCTAGTA TTGGCATGGT GCCAACAAGT GGTGCGATAA AACGTGATAT TCTGTCAGAG 
GAACACATAC AAAAATTAGA ACAGGAACTT CGAACGACAA AAGAGGATTT AAATAAAGAA 
CGTCAAGCCG TGAAACAAGC ACAAATTAAT TTATGGAAGA AAGAAAAAGA ATTATCGGAT 
GCAAATTTAG ATAAGAGAAT TGCAATAAGG TCAAATAAAA AGGCAGAGGA GAAAGTTAAA 
ATATTGCAAG AAGAAAAACA AAAATTATCA ATGAAATTAG ATGACAAAAT AAAAGAAGAA 
GAAGAGAAAT CCAAAAAATT GCTTAAAGAA TTAGACAGCG CAAAAGCATC ATTAAACGAT 
ATAACAAAAG AATCTTCTAG AAACAGAATG CAAGCTGATT CTGCTCAACG AGCGCTAACT 
CAAGCTAATC ATCAGATAGA AGAACTTCAA TCTTCAAGTG CTTTGCTGCG CAGAGAATTA 
GACGCTGCTC GAAAACAAGT AAGGGTAAGT CAAGATAGAC TGGAAAGTTT AAATGCTGAG 
AATAAGCGTT TGTCACAATC AATTACAAAA CATAACGAAG AAAAGAGTGC ATTGGAATCT 
AGAATAGAAA AGTTAGAACA AGAAATCAAA GGTTACGAAT TAAATACTGA ACTTCTAAAA 
GAAACATGTA CAGTTCTTGA AGAGCAACTT ACTGATTACG AAAGATTAAC TAGCGATCAT 
GAAACGCGAG AAAATATATT GATTCAAGAT AAAATGAAAT TGCAAAAAGA TTTAGAAGCA 
ACTGAAGCTA AACTTCGCGA AGCACGTGCT GCGCAAAATG AAGAAAAATC TATGAGATTA 
GCTGCGGAAC GTAACATCGA AAGACTTGAA TCGGAGACGA GCGATATAGA AAGTGAAAAG 
AACGGTTTAC TTGCTCAAAG AGATCAGTAT AGAAAGCTTG TTCAAGAATT GAGTGCACAG 
GTTGATGAAT TATCAAGTAA GTGCGGTGAA TTGGAATGTG ATCTTTCTGA AATGAGACGT 
AATTTAGAAG TGACCAAAGC TGAAACGAGA GTTGTCAAGG AAGAAAGTAG CCAACACTTA 
ACAAGAGTGC ACGAATTGAA AGAAGCAAAC ACCATGCTTA TGAACGACTT GCAGAGTAGC 
GTAGATCAGG GACAGGAGCT TCGAATGAGA ATTTCCGAAT TGGAAAGCAT CTTGGAAGAA 
ATGCGGCAAT TCTATCAAGA AAGAGAAGTA AAAGCTGAAA GTACAAGGCA ACAGCAGACA 
AAACTGATCG ATTATTTGCA GTTCAAATTA GAAGAATGTA GCAAAAAGAA GAAAACAGTA 
TGCAATAAAA TACTTGGTAC TAAACAAAAA GAAAATGTTC CGCCCTGTGG TACTGGTATG 
CCAGTTGGGT ATCGCGAATT AGAGAATCAG TTAACTAAAG AACGTGCTAA AGTTAAGACG 
TTAACTGATC AATTATTAGC GCTTAAAGCT ATGCATGCTT CAACCTCTGC CCCTACGTCT 
CCTTCAGCAC AGAGTATCAA GAATACAAAT TATATTTCTG AACAATCGGT CGACTCGTTA 
TCCAAACGAT TATCGCCCCA AAGAATTGGA CATAATATTC CACATCGTTT CGATGTCAGT 
TTACCTATGA GGGCTGGGAA ATGCTCCGCG TGTTTAGATC CTATTCAGTT CGGTAAACGT 
GCGGCTATTT GCAGTGAATG CGCAGTAATG ACGCACTTAA AATGTGCTGT TACTGTTCCC 
GCCACTTGCG GTTTGCCGGG CGGTTTCGCT AAACAATTCG GCAAAAGTTG GAGGAATAGC 
AATGACAGCT TGTCATCTTT ATCTGGAAGT GTTCAAACGT TAAATATAGA TCAACCGGAT 
AAACCTGATA CGGATATATG CAACGCACAA GATAAAGGTA ATAATGTATC AATGGAAAGT 
TGGGTCAAAG TTCCAGAGCG GTCAAAAAGC TGTTGGGAAA GAAAGTACCT TAGATTAGAA 
GGATCATGTT TATGTACTTA CGAGCACCAA CCATCTTCTG GGATGGCACC CATAAATCGT 
TTAGATTTGG TCGAAAAAGA TGGATTTAAT GTACTAGAAA ACGTACATCA ACCCGATGTA 
CAAGGAACAG CAAAGTCGGA TATACCATTT ATCTTTAGGA TAGAATCTAA TTCTTCCACG 
ACCTGTTGGC CTACATCGCG TTTAGATGTA ATGACTTTAA GTCAGACGGA TAAGAAAAAT 
TGGTTGAAAG CTTTGAAGTC TATTACTAGT CAAAATTATT CGAGTAACGC TTATAAAAGT 
AAAAAATATG AAATTGTATT AAGACTAGAA AAGAATCAGT TGGATTTGAA TTGTGCTGTA 
CATCTACCCG AAGATAATGT GTTGTTATTA GGCGCCGAAG AAGGACTCTT TTCATATTGC 
GGTGCTAAAT CACGAATGCT TACAGCGATT CGTGGAGTAA AGAGGGTTCA CCAATTAACG 
TTGCATCCTC ATTTGGGAAT AGCTTTAATG ATAGCCGGCG AAAATAGGCA ATTGGTATCT 
TGTAATCTGA GACAATTGAA GAGTAACGCA GTAGCTGCTG AGTGTTCGAG GCCAGCAATT 
AATACAAACC CAGTTTTAAC TGGCACTGAT AGTTGTCATT TATATCAGTT ACAAAGAGAT 
ATGCTTTGCG CCGCTACAGC AACTCACGTC ATATTACTTA AATGGTACAC CGAGGAAGAA 
GGAGGAGAAT TTGTTGGTGT ACGCGAACTT GAAACATCTG AGCCATGCAG CTGTGCCATA 
TTTACTCAAA ATGTTCTGGT AGTAGGATGC AACAAGTTCT TCCAAATCGA TCTGAGAAGC 
TATTGTGTCG ATGGATACAA GATGACGAAA GCAGTTCAGA GTTCAGTTTT ACATCAAGCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100878927 ( XM_012285728.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012141118.1 Megachile rotundata citron Rho-interacting kinase-like (LOC100878927), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMe105024
Clone ID Related Accession (Same CDS sequence)	XM_012285728.1
Accession Version	XM_012285728.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	citron Rho-interacting kinase-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797177.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTTG AAGACAGAGG GAAAGAATTT CAGGAGCGTT ATAAAAAGGT TTGTGGAAAA 
AGATTGTCTT CCGAATATGA GACTCAAACG AAGGAAAGCA CAAGAAAGAC GGAAACCTCC 
ATGAAAGAAA AAGAAAAGAT GGAAGAAGAA TTAAATGCAG CAAAATGTGA TTTAGCAAGT 
CTTCGAAAAA GTCTAAGTGG TGATAGAAAC ATTCCTGCAT CAGAATCTGA TGATCTTTCC 
AAAGATGTCC CAAAAGAATG TTCGTCTTTC TTTGAGTTTA AATTTCACAA ACATAATAAT 
GCGCAGTATC AAAAATATTC CAAAGAAAAG AGCGAATTGG AAGCAGAGTT AGATCTTAAA 
AAGAAAGAAT TACAGGAGAA GAAACAGGAA ATATTTGAAT TAAAGGAAAG AGTAAAAAGA 
TTAGAAGCAC TTAGTAAGGA AAGAGATATA CTTGAAAAGG AATTAATTAC CACGAAATCA 
GAATTAACTG GGATAAAAAG AACATTAGAA CTAGAACGGC AAGAGAGAAG AGATTTGGAG 
ACTAGAGCTC TCAGTTTGAT TAAAGATGCT AAGCGTAAAT GGGAAAATGC GGAAAAAGAT 
AAAATTGCAC AACTGAATAA ACATATTGAA ATGCAAACTG TAAGAATTAC AGAGTTATGT 
ACTAGTAATA ATGAAATGAG TTCAAGATTA CAAAGGACTG AATGTGAACT CGAAACTGCA 
AATGCAGAAT TGCATAAACT TCGAGTGTTT CAGATGCAAT ATAAAGAGTC GCTAGTTAAA 
ACTAGAGAAT TAAACCGGCA AAGTGCACAA GGAGTTGAAA CTAAATTGAA GGAAATAGCT 
GCAAGAGCAC ATAATCAGTT AGCTGAACTT AGATCGAAAT TAGATTTAGA AACAGCAAAA 
AATACTGACT TGGAAACTAA ACTACGAAAT GAACAAGATT CTAATCATTG CCGTCAATCA 
CGGTTAAATG TAGCTTTAGA GTTTGCACAA AATGAATTAA AGGACTGTCA GGAACAGTTA 
CGTACAATAC AAGCTACAAT ACCTGCCAGA GATGCTGAAA TAGACGTTTT AAAAAAACAA 
TTACAAGAAA GATCAAAACA ATTAGATAGT GCTATAGCAT CAGAACAGAT AGTAGCTACA 
ATGCAAGAGC AATTGGAGCG ATCAAGACTT GAGAATGAAC AATTGAAACA ACAGTTAGAG 
GTAATGAAGT CTGACTTAAG TGAAACAATG ATGAATTTAG AACAAAATGA AAACCTTGCT 
TTAAATTTAG AACAAGCTAC ACAAGATAAA GTTGCGCTCC AAAAACGACT ACAAGATTCT 
CTCGATAAAG AAGAAGAACA TTTGCGAAAA GTCGGTAATT TAGAAGAATT ATTAAGACGC 
TTAGAGCAAA GCGTTACTAA ACTCGAAACA GAAAATGCTA ATCTCAAAAT GGAAAATATA 
GAACCTAGTA TTGGCATGGT GCCAACAAGT GGTGCGATAA AACGTGATAT TCTGTCAGAG 
GAACACATAC AAAAATTAGA ACAGGAACTT CGAACGACAA AAGAGGATTT AAATAAAGAA 
CGTCAAGCCG TGAAACAAGC ACAAATTAAT TTATGGAAGA AAGAAAAAGA ATTATCGGAT 
GCAAATTTAG ATAAGAGAAT TGCAATAAGG TCAAATAAAA AGGCAGAGGA GAAAGTTAAA 
ATATTGCAAG AAGAAAAACA AAAATTATCA ATGAAATTAG ATGACAAAAT AAAAGAAGAA 
GAAGAGAAAT CCAAAAAATT GCTTAAAGAA TTAGACAGCG CAAAAGCATC ATTAAACGAT 
ATAACAAAAG AATCTTCTAG AAACAGAATG CAAGCTGATT CTGCTCAACG AGCGCTAACT 
CAAGCTAATC ATCAGATAGA AGAACTTCAA TCTTCAAGTG CTTTGCTGCG CAGAGAATTA 
GACGCTGCTC GAAAACAAGT AAGGGTAAGT CAAGATAGAC TGGAAAGTTT AAATGCTGAG 
AATAAGCGTT TGTCACAATC AATTACAAAA CATAACGAAG AAAAGAGTGC ATTGGAATCT 
AGAATAGAAA AGTTAGAACA AGAAATCAAA GGTTACGAAT TAAATACTGA ACTTCTAAAA 
GAAACATGTA CAGTTCTTGA AGAGCAACTT ACTGATTACG AAAGATTAAC TAGCGATCAT 
GAAACGCGAG AAAATATATT GATTCAAGAT AAAATGAAAT TGCAAAAAGA TTTAGAAGCA 
ACTGAAGCTA AACTTCGCGA AGCACGTGCT GCGCAAAATG AAGAAAAATC TATGAGATTA 
GCTGCGGAAC GTAACATCGA AAGACTTGAA TCGGAGACGA GCGATATAGA AAGTGAAAAG 
AACGGTTTAC TTGCTCAAAG AGATCAGTAT AGAAAGCTTG TTCAAGAATT GAGTGCACAG 
GTTGATGAAT TATCAAGTAA GTGCGGTGAA TTGGAATGTG ATCTTTCTGA AATGAGACGT 
AATTTAGAAG TGACCAAAGC TGAAACGAGA GTTGTCAAGG AAGAAAGTAG CCAACACTTA 
ACAAGAGTGC ACGAATTGAA AGAAGCAAAC ACCATGCTTA TGAACGACTT GCAGAGTAGC 
GTAGATCAGG GACAGGAGCT TCGAATGAGA ATTTCCGAAT TGGAAAGCAT CTTGGAAGAA 
ATGCGGCAAT TCTATCAAGA AAGAGAAGTA AAAGCTGAAA GTACAAGGCA ACAGCAGACA 
AAACTGATCG ATTATTTGCA GTTCAAATTA GAAGAATGTA GCAAAAAGAA GAAAACAGTA 
TGCAATAAAA TACTTGGTAC TAAACAAAAA GAAAATGTTC CGCCCTGTGG TACTGGTATG 
CCAGTTGGGT ATCGCGAATT AGAGAATCAG TTAACTAAAG AACGTGCTAA AGTTAAGACG 
TTAACTGATC AATTATTAGC GCTTAAAGCT ATGCATGCTT CAACCTCTGC CCCTACGTCT 
CCTTCAGCAC AGAGTATCAA GAATACAAAT TATATTTCTG AACAATCGGT CGACTCGTTA 
TCCAAACGAT TATCGCCCCA AAGAATTGGA CATAATATTC CACATCGTTT CGATGTCAGT 
TTACCTATGA GGGCTGGGAA ATGCTCCGCG TGTTTAGATC CTATTCAGTT CGGTAAACGT 
GCGGCTATTT GCAGTGAATG CGCAGTAATG ACGCACTTAA AATGTGCTGT TACTGTTCCC 
GCCACTTGCG GTTTGCCGGG CGGTTTCGCT AAACAATTCG GCAAAAGTTG GAGGAATAGC 
AATGACAGCT TGTCATCTTT ATCTGGAAGT GTTCAAACGT TAAATATAGA TCAACCGGAT 
AAACCTGATA CGGATATATG CAACGCACAA GATAAAGGTA ATAATGTATC AATGGAAAGT 
TGGGTCAAAG TTCCAGAGCG GTCAAAAAGC TGTTGGGAAA GAAAGTACCT TAGATTAGAA 
GGATCATGTT TATGTACTTA CGAGCACCAA CCATCTTCTG GGATGGCACC CATAAATCGT 
TTAGATTTGG TCGAAAAAGA TGGATTTAAT GTACTAGAAA ACGTACATCA ACCCGATGTA 
CAAGGAACAG CAAAGTCGGA TATACCATTT ATCTTTAGGA TAGAATCTAA TTCTTCCACG 
ACCTGTTGGC CTACATCGCG TTTAGATGTA ATGACTTTAA GTCAGACGGA TAAGAAAAAT 
TGGTTGAAAG CTTTGAAGTC TATTACTAGT CAAAATTATT CGAGTAACGC TTATAAAAGT 
AAAAAATATG AAATTGTATT AAGACTAGAA AAGAATCAGT TGGATTTGAA TTGTGCTGTA 
CATCTACCCG AAGATAATGT GTTGTTATTA GGCGCCGAAG AAGGACTCTT TTCATATTGC 
GGTGCTAAAT CACGAATGCT TACAGCGATT CGTGGAGTAA AGAGGGTTCA CCAATTAACG 
TTGCATCCTC ATTTGGGAAT AGCTTTAATG ATAGCCGGCG AAAATAGGCA ATTGGTATCT 
TGTAATCTGA GACAATTGAA GAGTAACGCA GTAGCTGCTG AGTGTTCGAG GCCAGCAATT 
AATACAAACC CAGTTTTAAC TGGCACTGAT AGTTGTCATT TATATCAGTT ACAAAGAGAT 
ATGCTTTGCG CCGCTACAGC AACTCACGTC ATATTACTTA AATGGTACAC CGAGGAAGAA 
GGAGGAGAAT TTGTTGGTGT ACGCGAACTT GAAACATCTG AGCCATGCAG CTGTGCCATA 
TTTACTCAAA ATGTTCTGGT AGTAGGATGC AACAAGTTCT TCCAAATCGA TCTGAGAAGC 
TATTGTGTCG ATGAGTTTCC AGAAGCAGAT GATAGTTCAG TTAAAGCTGC GCTATCAGGA 
GTAGCTAAAT TAGGTATCTT TCCCGTGTGT GTTTTGAACG TTTCTCTTGT ACCTGGAAAA 
ATAGAGCTTT TACTTTGTTA TAACGAGTTT GGAATGTTTG TGAATGAAAC CGGTCAAAGA 
ACTCGATGCA TCGATCCAAT GTGGAACCAT TTACCTTTTG CTTTTGCATT TCGTAAACCA 
TACTTGTTTA TTATTCACTT TTCATCCGTG GAAATTGTAA AACTCGATCA AGATGCATAC 
ACCTCACCGA CAAAAAATCC AGAACGAGTT TTAATCGAAT TATCTAGTCC TCGTTATTTA 
GGAATGTCTG AATCAAAAGG AATTTACATA GCAACCATTA ATTCTTTCTT AGAATTGCTA 
CAAATAGAAG GTCCTTCTAA TATACCAGAC AGCAATGGCA GTCTTACGAG TTTAGAAACC 
TTGATACAAG ATGACGAAAG CAGTTCAGAG TTCAGTTTTA CATCAAGCTT AATGGAAGAT 
TTAGACGGAC AAGGGAAGAG AGTACATTTC GCTGGTGTTA CTAAGTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012141118.1
CDS	446..5296
Translation	MDFEDRGKEFQERYKKVCGKRLSSEYETQTKESTRKTETSMKEKEKMEEELNAAKCDLASLRKSLSGDRNIPASESDDLSKDVPKECSSFFEFKFHKHNNAQYQKYSKEKSELEAELDLKKKELQEKKQEIFELKERVKRLEALSKERDILEKELITTKSELTGIKRTLELERQERRDLETRALSLIKDAKRKWENAEKDKIAQLNKHIEMQTVRITELCTSNNEMSSRLQRTECELETANAELHKLRVFQMQYKESLVKTRELNRQSAQGVETKLKEIAARAHNQLAELRSKLDLETAKNTDLETKLRNEQDSNHCRQSRLNVALEFAQNELKDCQEQLRTIQATIPARDAEIDVLKKQLQERSKQLDSAIASEQIVATMQEQLERSRLENEQLKQQLEVMKSDLSETMMNLEQNENLALNLEQATQDKVALQKRLQDSLDKEEEHLRKVGNLEELLRRLEQSVTKLETENANLKMENIEPSIGMVPTSGAIKRDILSEEHIQKLEQELRTTKEDLNKERQAVKQAQINLWKKEKELSDANLDKRIAIRSNKKAEEKVKILQEEKQKLSMKLDDKIKEEEEKSKKLLKELDSAKASLNDITKESSRNRMQADSAQRALTQANHQIEELQSSSALLRRELDAARKQVRVSQDRLESLNAENKRLSQSITKHNEEKSALESRIEKLEQEIKGYELNTELLKETCTVLEEQLTDYERLTSDHETRENILIQDKMKLQKDLEATEAKLREARAAQNEEKSMRLAAERNIERLESETSDIESEKNGLLAQRDQYRKLVQELSAQVDELSSKCGELECDLSEMRRNLEVTKAETRVVKEESSQHLTRVHELKEANTMLMNDLQSSVDQGQELRMRISELESILEEMRQFYQEREVKAESTRQQQTKLIDYLQFKLEECSKKKKTVCNKILGTKQKENVPPCGTGMPVGYRELENQLTKERAKVKTLTDQLLALKAMHASTSAPTSPSAQSIKNTNYISEQSVDSLSKRLSPQRIGHNIPHRFDVSLPMRAGKCSACLDPIQFGKRAAICSECAVMTHLKCAVTVPATCGLPGGFAKQFGKSWRNSNDSLSSLSGSVQTLNIDQPDKPDTDICNAQDKGNNVSMESWVKVPERSKSCWERKYLRLEGSCLCTYEHQPSSGMAPINRLDLVEKDGFNVLENVHQPDVQGTAKSDIPFIFRIESNSSTTCWPTSRLDVMTLSQTDKKNWLKALKSITSQNYSSNAYKSKKYEIVLRLEKNQLDLNCAVHLPEDNVLLLGAEEGLFSYCGAKSRMLTAIRGVKRVHQLTLHPHLGIALMIAGENRQLVSCNLRQLKSNAVAAECSRPAINTNPVLTGTDSCHLYQLQRDMLCAATATHVILLKWYTEEEGGEFVGVRELETSEPCSCAIFTQNVLVVGCNKFFQIDLRSYCVDEFPEADDSSVKAALSGVAKLGIFPVCVLNVSLVPGKIELLLCYNEFGMFVNETGQRTRCIDPMWNHLPFAFAFRKPYLFIIHFSSVEIVKLDQDAYTSPTKNPERVLIELSSPRYLGMSESKGIYIATINSFLELLQIEGPSNIPDSNGSLTSLETLIQDDESSSEFSFTSSLMEDLDGQGKRVHFAGVTKY

Target ORF information:

RefSeq Version	XM_012285728.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata citron Rho-interacting kinase-like (LOC100878927), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012285728.1

ORF Insert Sequence:

ATGGATTTTG AAGACAGAGG GAAAGAATTT CAGGAGCGTT ATAAAAAGGT TTGTGGAAAA 
AGATTGTCTT CCGAATATGA GACTCAAACG AAGGAAAGCA CAAGAAAGAC GGAAACCTCC 
ATGAAAGAAA AAGAAAAGAT GGAAGAAGAA TTAAATGCAG CAAAATGTGA TTTAGCAAGT 
CTTCGAAAAA GTCTAAGTGG TGATAGAAAC ATTCCTGCAT CAGAATCTGA TGATCTTTCC 
AAAGATGTCC CAAAAGAATG TTCGTCTTTC TTTGAGTTTA AATTTCACAA ACATAATAAT 
GCGCAGTATC AAAAATATTC CAAAGAAAAG AGCGAATTGG AAGCAGAGTT AGATCTTAAA 
AAGAAAGAAT TACAGGAGAA GAAACAGGAA ATATTTGAAT TAAAGGAAAG AGTAAAAAGA 
TTAGAAGCAC TTAGTAAGGA AAGAGATATA CTTGAAAAGG AATTAATTAC CACGAAATCA 
GAATTAACTG GGATAAAAAG AACATTAGAA CTAGAACGGC AAGAGAGAAG AGATTTGGAG 
ACTAGAGCTC TCAGTTTGAT TAAAGATGCT AAGCGTAAAT GGGAAAATGC GGAAAAAGAT 
AAAATTGCAC AACTGAATAA ACATATTGAA ATGCAAACTG TAAGAATTAC AGAGTTATGT 
ACTAGTAATA ATGAAATGAG TTCAAGATTA CAAAGGACTG AATGTGAACT CGAAACTGCA 
AATGCAGAAT TGCATAAACT TCGAGTGTTT CAGATGCAAT ATAAAGAGTC GCTAGTTAAA 
ACTAGAGAAT TAAACCGGCA AAGTGCACAA GGAGTTGAAA CTAAATTGAA GGAAATAGCT 
GCAAGAGCAC ATAATCAGTT AGCTGAACTT AGATCGAAAT TAGATTTAGA AACAGCAAAA 
AATACTGACT TGGAAACTAA ACTACGAAAT GAACAAGATT CTAATCATTG CCGTCAATCA 
CGGTTAAATG TAGCTTTAGA GTTTGCACAA AATGAATTAA AGGACTGTCA GGAACAGTTA 
CGTACAATAC AAGCTACAAT ACCTGCCAGA GATGCTGAAA TAGACGTTTT AAAAAAACAA 
TTACAAGAAA GATCAAAACA ATTAGATAGT GCTATAGCAT CAGAACAGAT AGTAGCTACA 
ATGCAAGAGC AATTGGAGCG ATCAAGACTT GAGAATGAAC AATTGAAACA ACAGTTAGAG 
GTAATGAAGT CTGACTTAAG TGAAACAATG ATGAATTTAG AACAAAATGA AAACCTTGCT 
TTAAATTTAG AACAAGCTAC ACAAGATAAA GTTGCGCTCC AAAAACGACT ACAAGATTCT 
CTCGATAAAG AAGAAGAACA TTTGCGAAAA GTCGGTAATT TAGAAGAATT ATTAAGACGC 
TTAGAGCAAA GCGTTACTAA ACTCGAAACA GAAAATGCTA ATCTCAAAAT GGAAAATATA 
GAACCTAGTA TTGGCATGGT GCCAACAAGT GGTGCGATAA AACGTGATAT TCTGTCAGAG 
GAACACATAC AAAAATTAGA ACAGGAACTT CGAACGACAA AAGAGGATTT AAATAAAGAA 
CGTCAAGCCG TGAAACAAGC ACAAATTAAT TTATGGAAGA AAGAAAAAGA ATTATCGGAT 
GCAAATTTAG ATAAGAGAAT TGCAATAAGG TCAAATAAAA AGGCAGAGGA GAAAGTTAAA 
ATATTGCAAG AAGAAAAACA AAAATTATCA ATGAAATTAG ATGACAAAAT AAAAGAAGAA 
GAAGAGAAAT CCAAAAAATT GCTTAAAGAA TTAGACAGCG CAAAAGCATC ATTAAACGAT 
ATAACAAAAG AATCTTCTAG AAACAGAATG CAAGCTGATT CTGCTCAACG AGCGCTAACT 
CAAGCTAATC ATCAGATAGA AGAACTTCAA TCTTCAAGTG CTTTGCTGCG CAGAGAATTA 
GACGCTGCTC GAAAACAAGT AAGGGTAAGT CAAGATAGAC TGGAAAGTTT AAATGCTGAG 
AATAAGCGTT TGTCACAATC AATTACAAAA CATAACGAAG AAAAGAGTGC ATTGGAATCT 
AGAATAGAAA AGTTAGAACA AGAAATCAAA GGTTACGAAT TAAATACTGA ACTTCTAAAA 
GAAACATGTA CAGTTCTTGA AGAGCAACTT ACTGATTACG AAAGATTAAC TAGCGATCAT 
GAAACGCGAG AAAATATATT GATTCAAGAT AAAATGAAAT TGCAAAAAGA TTTAGAAGCA 
ACTGAAGCTA AACTTCGCGA AGCACGTGCT GCGCAAAATG AAGAAAAATC TATGAGATTA 
GCTGCGGAAC GTAACATCGA AAGACTTGAA TCGGAGACGA GCGATATAGA AAGTGAAAAG 
AACGGTTTAC TTGCTCAAAG AGATCAGTAT AGAAAGCTTG TTCAAGAATT GAGTGCACAG 
GTTGATGAAT TATCAAGTAA GTGCGGTGAA TTGGAATGTG ATCTTTCTGA AATGAGACGT 
AATTTAGAAG TGACCAAAGC TGAAACGAGA GTTGTCAAGG AAGAAAGTAG CCAACACTTA 
ACAAGAGTGC ACGAATTGAA AGAAGCAAAC ACCATGCTTA TGAACGACTT GCAGAGTAGC 
GTAGATCAGG GACAGGAGCT TCGAATGAGA ATTTCCGAAT TGGAAAGCAT CTTGGAAGAA 
ATGCGGCAAT TCTATCAAGA AAGAGAAGTA AAAGCTGAAA GTACAAGGCA ACAGCAGACA 
AAACTGATCG ATTATTTGCA GTTCAAATTA GAAGAATGTA GCAAAAAGAA GAAAACAGTA 
TGCAATAAAA TACTTGGTAC TAAACAAAAA GAAAATGTTC CGCCCTGTGG TACTGGTATG 
CCAGTTGGGT ATCGCGAATT AGAGAATCAG TTAACTAAAG AACGTGCTAA AGTTAAGACG 
TTAACTGATC AATTATTAGC GCTTAAAGCT ATGCATGCTT CAACCTCTGC CCCTACGTCT 
CCTTCAGCAC AGAGTATCAA GAATACAAAT TATATTTCTG AACAATCGGT CGACTCGTTA 
TCCAAACGAT TATCGCCCCA AAGAATTGGA CATAATATTC CACATCGTTT CGATGTCAGT 
TTACCTATGA GGGCTGGGAA ATGCTCCGCG TGTTTAGATC CTATTCAGTT CGGTAAACGT 
GCGGCTATTT GCAGTGAATG CGCAGTAATG ACGCACTTAA AATGTGCTGT TACTGTTCCC 
GCCACTTGCG GTTTGCCGGG CGGTTTCGCT AAACAATTCG GCAAAAGTTG GAGGAATAGC 
AATGACAGCT TGTCATCTTT ATCTGGAAGT GTTCAAACGT TAAATATAGA TCAACCGGAT 
AAACCTGATA CGGATATATG CAACGCACAA GATAAAGGTA ATAATGTATC AATGGAAAGT 
TGGGTCAAAG TTCCAGAGCG GTCAAAAAGC TGTTGGGAAA GAAAGTACCT TAGATTAGAA 
GGATCATGTT TATGTACTTA CGAGCACCAA CCATCTTCTG GGATGGCACC CATAAATCGT 
TTAGATTTGG TCGAAAAAGA TGGATTTAAT GTACTAGAAA ACGTACATCA ACCCGATGTA 
CAAGGAACAG CAAAGTCGGA TATACCATTT ATCTTTAGGA TAGAATCTAA TTCTTCCACG 
ACCTGTTGGC CTACATCGCG TTTAGATGTA ATGACTTTAA GTCAGACGGA TAAGAAAAAT 
TGGTTGAAAG CTTTGAAGTC TATTACTAGT CAAAATTATT CGAGTAACGC TTATAAAAGT 
AAAAAATATG AAATTGTATT AAGACTAGAA AAGAATCAGT TGGATTTGAA TTGTGCTGTA 
CATCTACCCG AAGATAATGT GTTGTTATTA GGCGCCGAAG AAGGACTCTT TTCATATTGC 
GGTGCTAAAT CACGAATGCT TACAGCGATT CGTGGAGTAA AGAGGGTTCA CCAATTAACG 
TTGCATCCTC ATTTGGGAAT AGCTTTAATG ATAGCCGGCG AAAATAGGCA ATTGGTATCT 
TGTAATCTGA GACAATTGAA GAGTAACGCA GTAGCTGCTG AGTGTTCGAG GCCAGCAATT 
AATACAAACC CAGTTTTAAC TGGCACTGAT AGTTGTCATT TATATCAGTT ACAAAGAGAT 
ATGCTTTGCG CCGCTACAGC AACTCACGTC ATATTACTTA AATGGTACAC CGAGGAAGAA 
GGAGGAGAAT TTGTTGGTGT ACGCGAACTT GAAACATCTG AGCCATGCAG CTGTGCCATA 
TTTACTCAAA ATGTTCTGGT AGTAGGATGC AACAAGTTCT TCCAAATCGA TCTGAGAAGC 
TATTGTGTCG ATGAGTTTCC AGAAGCAGAT GATAGTTCAG TTAAAGCTGC GCTATCAGGA 
GTAGCTAAAT TAGGTATCTT TCCCGTGTGT GTTTTGAACG TTTCTCTTGT ACCTGGAAAA 
ATAGAGCTTT TACTTTGTTA TAACGAGTTT GGAATGTTTG TGAATGAAAC CGGTCAAAGA 
ACTCGATGCA TCGATCCAAT GTGGAACCAT TTACCTTTTG CTTTTGCATT TCGTAAACCA 
TACTTGTTTA TTATTCACTT TTCATCCGTG GAAATTGTAA AACTCGATCA AGATGCATAC 
ACCTCACCGA CAAAAAATCC AGAACGAGTT TTAATCGAAT TATCTAGTCC TCGTTATTTA 
GGAATGTCTG AATCAAAAGG AATTTACATA GCAACCATTA ATTCTTTCTT AGAATTGCTA 
CAAATAGAAG GTCCTTCTAA TATACCAGAC AGCAATGGCA GTCTTACGAG TTTAGAAACC 
TTGATACAAG ATGACGAAAG CAGTTCAGAG TTCAGTTTTA CATCAAGCTT AATGGAAGAT 
TTAGACGGAC AAGGGAAGAG AGTACATTTC GCTGGTGTTA CTAAGTACTG A