LOC100878636 gene cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

mRNA	Protein	Name
XM_003703721.2	XP_003703769.1	tyrosine-protein kinase Abl isoform X1
XM_012286435.1	XP_012141825.1	tyrosine-protein kinase Abl isoform X3
XM_012286439.1	XP_012141829.1	tyrosine-protein kinase Abl isoform X6
XM_012286434.1	XP_012141824.1	tyrosine-protein kinase Abl isoform X2
XM_012286437.1	XP_012141827.1	tyrosine-protein kinase Abl isoform X5
XM_012286440.1	XP_012141830.1	tyrosine-protein kinase Abl isoform X7
XM_012286436.1	XP_012141826.1	tyrosine-protein kinase Abl isoform X4
XM_012286441.1	XP_012141831.1	tyrosine-protein kinase Abl isoform X8
XM_012286442.1	XP_012141832.1	tyrosine-protein kinase Abl isoform X9

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMe105642	XM_003703721.2 Latest version!	Megachile rotundata tyrosine-protein kinase Abl (LOC100878636), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OMe105643	XM_012286434.1 Latest version!	Megachile rotundata tyrosine-protein kinase Abl (LOC100878636), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OMe105644	XM_012286435.1 Latest version!	Megachile rotundata tyrosine-protein kinase Abl (LOC100878636), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OMe105645	XM_012286436.1 Latest version!	Megachile rotundata tyrosine-protein kinase Abl (LOC100878636), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OMe105646	XM_012286437.1 Latest version!	Megachile rotundata tyrosine-protein kinase Abl (LOC100878636), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OMe105647	XM_012286439.1 Latest version!	Megachile rotundata tyrosine-protein kinase Abl (LOC100878636), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OMe105648	XM_012286440.1 Latest version!	Megachile rotundata tyrosine-protein kinase Abl (LOC100878636), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OMe105649	XM_012286441.1 Latest version!	Megachile rotundata tyrosine-protein kinase Abl (LOC100878636), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OMe105650	XM_012286442.1 Latest version!	Megachile rotundata tyrosine-protein kinase Abl (LOC100878636), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

LOC100878636 ( XM_003703721.2 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_003703769.1 Megachile rotundata tyrosine-protein kinase Abl (LOC100878636), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMe105642
Clone ID Related Accession (Same CDS sequence)	XM_003703721.2
Accession Version	XM_003703721.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	tyrosine-protein kinase Abl isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797180.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2015 this sequence version replaced XM_003703721.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGCTC AACAGACTAA GGAAAGAATT GTTCCTGCCG GCTCTACTGC GCGACAGACT 
CGCAAACAGC CAAGGAACCT CAAGGAGTCT CGTCTTGTTG GTTCCAATAT ATTTACAGAA 
CACAGCGAAG CTCTCTTACA AAGCAGACCG CTACCTCACA TTCCGGCATT ACCAGATGGC 
GATCCTCCTA GTGGCTCTGC TATCCAACCA ATTTCACAAC AAGTGAATAT TCAACAACAT 
GCTGGTGTTT CATCTACAGG GCTTTTGGAA GCTGCAAACA GGTGGACAAG TAAAGAAAAC 
TTATTAGCAC AGGAGGAAGA TGATCCACAA CTATTTGTGG CTCTATATGA TTTTCAAGCA 
GGTGGAGAAA ACCAACTTAG TCTTAAAAAG GGAGAGCAAG TTCGTATTCT AAGTTATAAT 
AAAAGTGGAG AATGGTGTGA AGCTCATTCA AGTACTGGTC AAGTGGGGTG GGTTCCTTCA 
AATTATGTCA CCCCTGTAAA TTCTTTGGAA AAACATTCTT GGTATCATGG AAGGATATCC 
AGAAATGCTG CTGAATATCT CCTAAGTTCT GGTATAAATG GTAGCTTTCT GGTTCGTGAA 
TCAGAAAGCA GTCCAGGTCA ACGCAGTATT TCTTTGAGAT ATGAGGGTAG AGTCTATCAT 
TATAGGATTA ATGAGGATAG TGAAGGAAAG ATGTTTGTCA CAACAGAAAG CAAATTTAAT 
ACTCTGGCAG AATTGGTACA TCATCATTCT ATGCTTGCTG ATGGTTTAAT CACACAGCTA 
CTTTATCCTG CACCAAAGCA TAACAAACCA ACTGTTTTTC CACTTAGTCC AGAACCAGAT 
GAATGGGAGA TTAATAGGAC AGATATAGTT ATGAGGCATA AGTTAGGTGG AGGACAATAT 
GGAGATGTCT ATGAAGCAGT ATGGAAAAGA TATAACATGA CTGTGGCTGT AAAAACATTA 
AAAGAAGACA CAATGGCCCT AAAGGACTTT TTGGAGGAAG CTGCAATCAT GAAGGAGATG 
AAGCATAGGA ATTTAGTTCA GCTACTGGGT GTATGTACTC GAGAACCACC TTTCTACATC 
ATTACAGAGT TTATGAGCAA AGGAAATCTG CTAGACTATT TACGGAACGA GAGTAAACAT 
CAAATCAATG CTGTCGTTTT GATGCACATG GCCACACAGA TTGCTAGTGG AATGAGTTAT 
TTGGAGAGTA GAAATTTTAT ACACAGAGAT CTAGCAGCTC GAAACTGCCT AGTGGGTGAA 
AACCATCTGG TAAAGGTCGC AGACTTTGGC TTGGCCCGGT TGATGAGAGA TGATACGTAT 
ACGGCTCACG CTGGAGCAAA GTTTCCAATA AAATGGACTG CTCCAGAAGG ATTAGCTTAC 
AATAAATTCT CTACGAAGTC TGATGTGTGG GCATTTGGAA TTTTACTTTG GGAGATAGCA 
ACCTATGGCA TGTCCCCATA CCCTGGTGTT GATTTGACGG ATGTGTATCA CATGTTGGAG 
AAAGGCTACC GAATGGAATG TCCACCTGGA TGTCCACCGA AGGTATATGA ACTGATGCGT 
CAATGCTGGC AGTGGGCTGC TGCTGACCGG CCTACCTTCA AAGAGATTCA TCATTCTCTT 
GAAAATATGT TCCAAGAATC CAGTATTACC GAGGAAGTTG AAAAACAACT GCAAGGTGGC 
GGCGATATCC CTTTACTTTC ATATAAAAAA TCTCAAACTG GTAGTACTGG AAACATACAT 
GGGCTTGTTC TAGTCTCCGA GCCATTACCC TCTTCAGAAA CTACTAGCTC GGTAACAAAA 
CTGAGTACGT TTACGGGTGG TCTATCGAGT AAAAATAACA GCAATATCGT CCAAATGAGG 
CGTTCTACGA ATAAGAAAGG AAAACAAGCT CCGGCACCTC CAAAGAGAAC GAGCCTGCTG 
TCGTCGTGCA GTTCGTTTCG CGATTCTGCC TACCAAGAGC AAGATACCCA AAATACTGAA 
ACAAACACCA TGACTCTCGA CGATGCCACG GATCTAAATG GTATTGATAA GATTCTCGAA 
GGTATTGCGC GAGATCTCGC GACGATGGCT CAAGGAACGA TTGCTGCAGG AGGCTGCGAA 
GTCGAGGAGG ATGGAGAGGG TTCTCAAGGT ACGGCGGAAC CAAATTTTAT CCCTCAACCT 
TCGACGTCCC CGGAGCCTAT ACCTGGACTA GTAGCCTGTT CTCAGAAACA AATCAAACCA 
CGACCTTATC CATCAAAGGA ACCGTTACCA CAGAAGCTGG TACAAGTGGG AGCATTAGAA 
GTACAGAACG TAAAAAGAGC CATTAATCGT TATGGCACAC TACCAAAAGG TGCTAGGATC 
GGAGCATACC TAGAATCTTT GCGTCAAAGT GGTATGCCAT CGAATCAAGA ATCTACAACG 
GTGGTTTCTT CCATTGCACC TAGTTCAGTA GAACAACATG AGACTTCTAT GGACAATTCG 
CAACATAGAT CTCTCTCTCC TCGTCAAAGC AATCTACGAA ATCAACCGCA AATGACTCGC 
AGTAATTCCT CGAGCGGTGT TGTCAATACC TACCAACCTC CGAATTCCCC TCGTGGCCGA 
GTAGTTACTG TAAGAAAGAA CAATCAGTCT GATGGTGTTG GTCTGAGAAC ATTTCGAGTT 
TCAAGTAACT CGAACTTCAG AACTGCAAGT CCATCAAGAT CCGTTCAACC ATCTTTAGCG 
GATCTAGAGT TTCCGCCCCC ACCCGCAGAT TTGCCTCCTC CCCCTGACGA AACTTTCTCT 
GGTCAAGAAC AAGCTGAACT TCCGCCTCCC GTTTCTTGTA CCGAGCTATC TCAGATAAGA 
AATTCTCCTC TTTCTATAAG AAAAGCAAAG AGCACGGATT GGCGCACGAA AGAAGAAGAT 
AATGAACAGG AAGACAGGAA CGACGTTAGT AACATAGAGC CTTCCGTCAA AGAAGCTTGT 
TCGAGGTTTG GAGTTAATTT AAGGCGCAGA GAAACTCAAG ATAATTCTTG TAGAAACTCT 
GATAATAAAA GAACGGGATT TAAATCCAGA ATGGAGACAG TAGAGCCTGC TGCACCGCCA 
GAGGAAGCAC CACCTCCCCC ACCGCCACCT CCGCCGCCAC TTTCTACGAA TACACCCCCC 
GATAGCTTCG AACGTAAACC AGGCATGAAA GAAATGTTAG AATTAAAATT GATCAATGAA 
ATTAAACAGA GCGCGGAAAC AAAACATGGT ACGACTACGA AAAAGACTGG AGCGACAAGC 
AATACTCCAT CCGCACCTTT AGATCCAGCG TCGCAGCTAC TCTCGGAACT TTGTGCTAGT 
TTTAATATGG AGTCTGGACA GAGACACGCC CAAAACGAGT ACGCTGTATC AACTTTAAAA 
ACCAATGACG TAAATCAAGA ACAACAACAG CAACAAAGTC ATAACGCTCA TAAGGATTCG 
TCGGTGTCCT CTCCAGTGAC CGAGTCTCTC CTGTCCAGTG GAAATGTTGG ATTCAAGCTG 
AAAAGAGTTG ACAAGCGGAA TAATCCGCAA AAAGAAGAAA CATCCGATGG ACAAATAATT 
GATTTTAAAG CGAGACTTCG AAAAGTTGAG AACGCAGAAA AAGAAAAATC CTTGGAAGAG 
AAAAGTACCG TCACTGAACA GTCTTCAGAA TCAGAAGAGC AACAAGATGA CAAACGTAGA 
AGCACTGGTA GTATCAGTAG TTTAAAGAAG CTTTGGGAGA ACAAAGAATC TTGTGACAGT 
CAGCCTCATA GTCCAAAACT CAATGTCAGA GGAAGTGGTG GTAAACAAGA AATGATAGAC 
CAGACAGAAG ACTCACCGGA GGATCATAGT GGAGCTTCGA CACGTAGTCA GAGTTCCGGT 
AGCAAAAGTG ACGCTAGACT ATGGCCACCG CCCGAACCTG AAAAACCGGC AGTTCCAGCA 
AAGCCATTAA AACCACTATG TTCATCAACG AAGCACTTTG GTTCTTCGAT TTACGCAACG 
CCAAACTGTA CGAAATCTCA GCACGCTGAG GACGACCTAA ACAAACAGAA TACAGATTCA 
AAGACTGCGA AACAGGCAGT ATTGGAGCTT TCGACGTTGA TTGAGAGCAG CATATTAAAC 
TTGAAAAGTA GTCCAACAAT TGTAATGACA AGTTGGCTTC AGCTCTCTGA CAAGGTAGGG 
CTACTACACG GAATGTGCAC GAATCTCGCA GACAGTGGTA TCGCACCACA TGCGCGGTTT 
CAATTTCGCG AACTTCTTGC AAGATTAGAA CTTCAGGCAC GGCAGCTTCG TGCAGCCGGT 
ATTCGGAACG TTGCAGAGAA TACAAGGCTT CTCACTGATC TTCAAAACAC GATAAAGGAT 
GTTGTTAATG CTGTGCAAAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003703769.1
CDS	278..4621
Translation	MGAQQTKERIVPAGSTARQTRKQPRNLKESRLVGSNIFTEHSEALLQSRPLPHIPALPDGDPPSGSAIQPISQQVNIQQHAGVSSTGLLEAANRWTSKENLLAQEEDDPQLFVALYDFQAGGENQLSLKKGEQVRILSYNKSGEWCEAHSSTGQVGWVPSNYVTPVNSLEKHSWYHGRISRNAAEYLLSSGINGSFLVRESESSPGQRSISLRYEGRVYHYRINEDSEGKMFVTTESKFNTLAELVHHHSMLADGLITQLLYPAPKHNKPTVFPLSPEPDEWEINRTDIVMRHKLGGGQYGDVYEAVWKRYNMTVAVKTLKEDTMALKDFLEEAAIMKEMKHRNLVQLLGVCTREPPFYIITEFMSKGNLLDYLRNESKHQINAVVLMHMATQIASGMSYLESRNFIHRDLAARNCLVGENHLVKVADFGLARLMRDDTYTAHAGAKFPIKWTAPEGLAYNKFSTKSDVWAFGILLWEIATYGMSPYPGVDLTDVYHMLEKGYRMECPPGCPPKVYELMRQCWQWAAADRPTFKEIHHSLENMFQESSITEEVEKQLQGGGDIPLLSYKKSQTGSTGNIHGLVLVSEPLPSSETTSSVTKLSTFTGGLSSKNNSNIVQMRRSTNKKGKQAPAPPKRTSLLSSCSSFRDSAYQEQDTQNTETNTMTLDDATDLNGIDKILEGIARDLATMAQGTIAAGGCEVEEDGEGSQGTAEPNFIPQPSTSPEPIPGLVACSQKQIKPRPYPSKEPLPQKLVQVGALEVQNVKRAINRYGTLPKGARIGAYLESLRQSGMPSNQESTTVVSSIAPSSVEQHETSMDNSQHRSLSPRQSNLRNQPQMTRSNSSSGVVNTYQPPNSPRGRVVTVRKNNQSDGVGLRTFRVSSNSNFRTASPSRSVQPSLADLEFPPPPADLPPPPDETFSGQEQAELPPPVSCTELSQIRNSPLSIRKAKSTDWRTKEEDNEQEDRNDVSNIEPSVKEACSRFGVNLRRRETQDNSCRNSDNKRTGFKSRMETVEPAAPPEEAPPPPPPPPPPLSTNTPPDSFERKPGMKEMLELKLINEIKQSAETKHGTTTKKTGATSNTPSAPLDPASQLLSELCASFNMESGQRHAQNEYAVSTLKTNDVNQEQQQQQSHNAHKDSSVSSPVTESLLSSGNVGFKLKRVDKRNNPQKEETSDGQIIDFKARLRKVENAEKEKSLEEKSTVTEQSSESEEQQDDKRRSTGSISSLKKLWENKESCDSQPHSPKLNVRGSGGKQEMIDQTEDSPEDHSGASTRSQSSGSKSDARLWPPPEPEKPAVPAKPLKPLCSSTKHFGSSIYATPNCTKSQHAEDDLNKQNTDSKTAKQAVLELSTLIESSILNLKSSPTIVMTSWLQLSDKVGLLHGMCTNLADSGIAPHARFQFRELLARLELQARQLRAAGIRNVAENTRLLTDLQNTIKDVVNAVQR

Target ORF information:

RefSeq Version	XM_003703721.2
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata tyrosine-protein kinase Abl (LOC100878636), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003703721.2

ORF Insert Sequence:

ATGGGTGCTC AACAGACTAA GGAAAGAATT GTTCCTGCCG GCTCTACTGC GCGACAGACT 
CGCAAACAGC CAAGGAACCT CAAGGAGTCT CGTCTTGTTG GTTCCAATAT ATTTACAGAA 
CACAGCGAAG CTCTCTTACA AAGCAGACCG CTACCTCACA TTCCGGCATT ACCAGATGGC 
GATCCTCCTA GTGGCTCTGC TATCCAACCA ATTTCACAAC AAGTGAATAT TCAACAACAT 
GCTGGTGTTT CATCTACAGG GCTTTTGGAA GCTGCAAACA GGTGGACAAG TAAAGAAAAC 
TTATTAGCAC AGGAGGAAGA TGATCCACAA CTATTTGTGG CTCTATATGA TTTTCAAGCA 
GGTGGAGAAA ACCAACTTAG TCTTAAAAAG GGAGAGCAAG TTCGTATTCT AAGTTATAAT 
AAAAGTGGAG AATGGTGTGA AGCTCATTCA AGTACTGGTC AAGTGGGGTG GGTTCCTTCA 
AATTATGTCA CCCCTGTAAA TTCTTTGGAA AAACATTCTT GGTATCATGG AAGGATATCC 
AGAAATGCTG CTGAATATCT CCTAAGTTCT GGTATAAATG GTAGCTTTCT GGTTCGTGAA 
TCAGAAAGCA GTCCAGGTCA ACGCAGTATT TCTTTGAGAT ATGAGGGTAG AGTCTATCAT 
TATAGGATTA ATGAGGATAG TGAAGGAAAG ATGTTTGTCA CAACAGAAAG CAAATTTAAT 
ACTCTGGCAG AATTGGTACA TCATCATTCT ATGCTTGCTG ATGGTTTAAT CACACAGCTA 
CTTTATCCTG CACCAAAGCA TAACAAACCA ACTGTTTTTC CACTTAGTCC AGAACCAGAT 
GAATGGGAGA TTAATAGGAC AGATATAGTT ATGAGGCATA AGTTAGGTGG AGGACAATAT 
GGAGATGTCT ATGAAGCAGT ATGGAAAAGA TATAACATGA CTGTGGCTGT AAAAACATTA 
AAAGAAGACA CAATGGCCCT AAAGGACTTT TTGGAGGAAG CTGCAATCAT GAAGGAGATG 
AAGCATAGGA ATTTAGTTCA GCTACTGGGT GTATGTACTC GAGAACCACC TTTCTACATC 
ATTACAGAGT TTATGAGCAA AGGAAATCTG CTAGACTATT TACGGAACGA GAGTAAACAT 
CAAATCAATG CTGTCGTTTT GATGCACATG GCCACACAGA TTGCTAGTGG AATGAGTTAT 
TTGGAGAGTA GAAATTTTAT ACACAGAGAT CTAGCAGCTC GAAACTGCCT AGTGGGTGAA 
AACCATCTGG TAAAGGTCGC AGACTTTGGC TTGGCCCGGT TGATGAGAGA TGATACGTAT 
ACGGCTCACG CTGGAGCAAA GTTTCCAATA AAATGGACTG CTCCAGAAGG ATTAGCTTAC 
AATAAATTCT CTACGAAGTC TGATGTGTGG GCATTTGGAA TTTTACTTTG GGAGATAGCA 
ACCTATGGCA TGTCCCCATA CCCTGGTGTT GATTTGACGG ATGTGTATCA CATGTTGGAG 
AAAGGCTACC GAATGGAATG TCCACCTGGA TGTCCACCGA AGGTATATGA ACTGATGCGT 
CAATGCTGGC AGTGGGCTGC TGCTGACCGG CCTACCTTCA AAGAGATTCA TCATTCTCTT 
GAAAATATGT TCCAAGAATC CAGTATTACC GAGGAAGTTG AAAAACAACT GCAAGGTGGC 
GGCGATATCC CTTTACTTTC ATATAAAAAA TCTCAAACTG GTAGTACTGG AAACATACAT 
GGGCTTGTTC TAGTCTCCGA GCCATTACCC TCTTCAGAAA CTACTAGCTC GGTAACAAAA 
CTGAGTACGT TTACGGGTGG TCTATCGAGT AAAAATAACA GCAATATCGT CCAAATGAGG 
CGTTCTACGA ATAAGAAAGG AAAACAAGCT CCGGCACCTC CAAAGAGAAC GAGCCTGCTG 
TCGTCGTGCA GTTCGTTTCG CGATTCTGCC TACCAAGAGC AAGATACCCA AAATACTGAA 
ACAAACACCA TGACTCTCGA CGATGCCACG GATCTAAATG GTATTGATAA GATTCTCGAA 
GGTATTGCGC GAGATCTCGC GACGATGGCT CAAGGAACGA TTGCTGCAGG AGGCTGCGAA 
GTCGAGGAGG ATGGAGAGGG TTCTCAAGGT ACGGCGGAAC CAAATTTTAT CCCTCAACCT 
TCGACGTCCC CGGAGCCTAT ACCTGGACTA GTAGCCTGTT CTCAGAAACA AATCAAACCA 
CGACCTTATC CATCAAAGGA ACCGTTACCA CAGAAGCTGG TACAAGTGGG AGCATTAGAA 
GTACAGAACG TAAAAAGAGC CATTAATCGT TATGGCACAC TACCAAAAGG TGCTAGGATC 
GGAGCATACC TAGAATCTTT GCGTCAAAGT GGTATGCCAT CGAATCAAGA ATCTACAACG 
GTGGTTTCTT CCATTGCACC TAGTTCAGTA GAACAACATG AGACTTCTAT GGACAATTCG 
CAACATAGAT CTCTCTCTCC TCGTCAAAGC AATCTACGAA ATCAACCGCA AATGACTCGC 
AGTAATTCCT CGAGCGGTGT TGTCAATACC TACCAACCTC CGAATTCCCC TCGTGGCCGA 
GTAGTTACTG TAAGAAAGAA CAATCAGTCT GATGGTGTTG GTCTGAGAAC ATTTCGAGTT 
TCAAGTAACT CGAACTTCAG AACTGCAAGT CCATCAAGAT CCGTTCAACC ATCTTTAGCG 
GATCTAGAGT TTCCGCCCCC ACCCGCAGAT TTGCCTCCTC CCCCTGACGA AACTTTCTCT 
GGTCAAGAAC AAGCTGAACT TCCGCCTCCC GTTTCTTGTA CCGAGCTATC TCAGATAAGA 
AATTCTCCTC TTTCTATAAG AAAAGCAAAG AGCACGGATT GGCGCACGAA AGAAGAAGAT 
AATGAACAGG AAGACAGGAA CGACGTTAGT AACATAGAGC CTTCCGTCAA AGAAGCTTGT 
TCGAGGTTTG GAGTTAATTT AAGGCGCAGA GAAACTCAAG ATAATTCTTG TAGAAACTCT 
GATAATAAAA GAACGGGATT TAAATCCAGA ATGGAGACAG TAGAGCCTGC TGCACCGCCA 
GAGGAAGCAC CACCTCCCCC ACCGCCACCT CCGCCGCCAC TTTCTACGAA TACACCCCCC 
GATAGCTTCG AACGTAAACC AGGCATGAAA GAAATGTTAG AATTAAAATT GATCAATGAA 
ATTAAACAGA GCGCGGAAAC AAAACATGGT ACGACTACGA AAAAGACTGG AGCGACAAGC 
AATACTCCAT CCGCACCTTT AGATCCAGCG TCGCAGCTAC TCTCGGAACT TTGTGCTAGT 
TTTAATATGG AGTCTGGACA GAGACACGCC CAAAACGAGT ACGCTGTATC AACTTTAAAA 
ACCAATGACG TAAATCAAGA ACAACAACAG CAACAAAGTC ATAACGCTCA TAAGGATTCG 
TCGGTGTCCT CTCCAGTGAC CGAGTCTCTC CTGTCCAGTG GAAATGTTGG ATTCAAGCTG 
AAAAGAGTTG ACAAGCGGAA TAATCCGCAA AAAGAAGAAA CATCCGATGG ACAAATAATT 
GATTTTAAAG CGAGACTTCG AAAAGTTGAG AACGCAGAAA AAGAAAAATC CTTGGAAGAG 
AAAAGTACCG TCACTGAACA GTCTTCAGAA TCAGAAGAGC AACAAGATGA CAAACGTAGA 
AGCACTGGTA GTATCAGTAG TTTAAAGAAG CTTTGGGAGA ACAAAGAATC TTGTGACAGT 
CAGCCTCATA GTCCAAAACT CAATGTCAGA GGAAGTGGTG GTAAACAAGA AATGATAGAC 
CAGACAGAAG ACTCACCGGA GGATCATAGT GGAGCTTCGA CACGTAGTCA GAGTTCCGGT 
AGCAAAAGTG ACGCTAGACT ATGGCCACCG CCCGAACCTG AAAAACCGGC AGTTCCAGCA 
AAGCCATTAA AACCACTATG TTCATCAACG AAGCACTTTG GTTCTTCGAT TTACGCAACG 
CCAAACTGTA CGAAATCTCA GCACGCTGAG GACGACCTAA ACAAACAGAA TACAGATTCA 
AAGACTGCGA AACAGGCAGT ATTGGAGCTT TCGACGTTGA TTGAGAGCAG CATATTAAAC 
TTGAAAAGTA GTCCAACAAT TGTAATGACA AGTTGGCTTC AGCTCTCTGA CAAGGTAGGG 
CTACTACACG GAATGTGCAC GAATCTCGCA GACAGTGGTA TCGCACCACA TGCGCGGTTT 
CAATTTCGCG AACTTCTTGC AAGATTAGAA CTTCAGGCAC GGCAGCTTCG TGCAGCCGGT 
ATTCGGAACG TTGCAGAGAA TACAAGGCTT CTCACTGATC TTCAAAACAC GATAAAGGAT 
GTTGTTAATG CTGTGCAAAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100878636 ( XM_012286434.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012141824.1 Megachile rotundata tyrosine-protein kinase Abl (LOC100878636), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMe105643
Clone ID Related Accession (Same CDS sequence)	XM_012286434.1
Accession Version	XM_012286434.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4341bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	tyrosine-protein kinase Abl isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797180.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGCTC AACAGACTAA GGAAAGAATT GTTCCTGCCG GCTCTACTGC GCGACAGACT 
CGCAAACAGC CAAGGAACCT CAAGGAGTCT CGTCTTGTTG GTTCCAATAT ATTTACAGAA 
CACAGCGCTC TCTTACAAAG CAGACCGCTA CCTCACATTC CGGCATTACC AGATGGCGAT 
CCTCCTAGTG GCTCTGCTAT CCAACCAATT TCACAACAAG TGAATATTCA ACAACATGCT 
GGTGTTTCAT CTACAGGGCT TTTGGAAGCT GCAAACAGGT GGACAAGTAA AGAAAACTTA 
TTAGCACAGG AGGAAGATGA TCCACAACTA TTTGTGGCTC TATATGATTT TCAAGCAGGT 
GGAGAAAACC AACTTAGTCT TAAAAAGGGA GAGCAAGTTC GTATTCTAAG TTATAATAAA 
AGTGGAGAAT GGTGTGAAGC TCATTCAAGT ACTGGTCAAG TGGGGTGGGT TCCTTCAAAT 
TATGTCACCC CTGTAAATTC TTTGGAAAAA CATTCTTGGT ATCATGGAAG GATATCCAGA 
AATGCTGCTG AATATCTCCT AAGTTCTGGT ATAAATGGTA GCTTTCTGGT TCGTGAATCA 
GAAAGCAGTC CAGGTCAACG CAGTATTTCT TTGAGATATG AGGGTAGAGT CTATCATTAT 
AGGATTAATG AGGATAGTGA AGGAAAGATG TTTGTCACAA CAGAAAGCAA ATTTAATACT 
CTGGCAGAAT TGGTACATCA TCATTCTATG CTTGCTGATG GTTTAATCAC ACAGCTACTT 
TATCCTGCAC CAAAGCATAA CAAACCAACT GTTTTTCCAC TTAGTCCAGA ACCAGATGAA 
TGGGAGATTA ATAGGACAGA TATAGTTATG AGGCATAAGT TAGGTGGAGG ACAATATGGA 
GATGTCTATG AAGCAGTATG GAAAAGATAT AACATGACTG TGGCTGTAAA AACATTAAAA 
GAAGACACAA TGGCCCTAAA GGACTTTTTG GAGGAAGCTG CAATCATGAA GGAGATGAAG 
CATAGGAATT TAGTTCAGCT ACTGGGTGTA TGTACTCGAG AACCACCTTT CTACATCATT 
ACAGAGTTTA TGAGCAAAGG AAATCTGCTA GACTATTTAC GGAACGAGAG TAAACATCAA 
ATCAATGCTG TCGTTTTGAT GCACATGGCC ACACAGATTG CTAGTGGAAT GAGTTATTTG 
GAGAGTAGAA ATTTTATACA CAGAGATCTA GCAGCTCGAA ACTGCCTAGT GGGTGAAAAC 
CATCTGGTAA AGGTCGCAGA CTTTGGCTTG GCCCGGTTGA TGAGAGATGA TACGTATACG 
GCTCACGCTG GAGCAAAGTT TCCAATAAAA TGGACTGCTC CAGAAGGATT AGCTTACAAT 
AAATTCTCTA CGAAGTCTGA TGTGTGGGCA TTTGGAATTT TACTTTGGGA GATAGCAACC 
TATGGCATGT CCCCATACCC TGGTGTTGAT TTGACGGATG TGTATCACAT GTTGGAGAAA 
GGCTACCGAA TGGAATGTCC ACCTGGATGT CCACCGAAGG TATATGAACT GATGCGTCAA 
TGCTGGCAGT GGGCTGCTGC TGACCGGCCT ACCTTCAAAG AGATTCATCA TTCTCTTGAA 
AATATGTTCC AAGAATCCAG TATTACCGAG GAAGTTGAAA AACAACTGCA AGGTGGCGGC 
GATATCCCTT TACTTTCATA TAAAAAATCT CAAACTGGTA GTACTGGAAA CATACATGGG 
CTTGTTCTAG TCTCCGAGCC ATTACCCTCT TCAGAAACTA CTAGCTCGGT AACAAAACTG 
AGTACGTTTA CGGGTGGTCT ATCGAGTAAA AATAACAGCA ATATCGTCCA AATGAGGCGT 
TCTACGAATA AGAAAGGAAA ACAAGCTCCG GCACCTCCAA AGAGAACGAG CCTGCTGTCG 
TCGTGCAGTT CGTTTCGCGA TTCTGCCTAC CAAGAGCAAG ATACCCAAAA TACTGAAACA 
AACACCATGA CTCTCGACGA TGCCACGGAT CTAAATGGTA TTGATAAGAT TCTCGAAGGT 
ATTGCGCGAG ATCTCGCGAC GATGGCTCAA GGAACGATTG CTGCAGGAGG CTGCGAAGTC 
GAGGAGGATG GAGAGGGTTC TCAAGGTACG GCGGAACCAA ATTTTATCCC TCAACCTTCG 
ACGTCCCCGG AGCCTATACC TGGACTAGTA GCCTGTTCTC AGAAACAAAT CAAACCACGA 
CCTTATCCAT CAAAGGAACC GTTACCACAG AAGCTGGTAC AAGTGGGAGC ATTAGAAGTA 
CAGAACGTAA AAAGAGCCAT TAATCGTTAT GGCACACTAC CAAAAGGTGC TAGGATCGGA 
GCATACCTAG AATCTTTGCG TCAAAGTGGT ATGCCATCGA ATCAAGAATC TACAACGGTG 
GTTTCTTCCA TTGCACCTAG TTCAGTAGAA CAACATGAGA CTTCTATGGA CAATTCGCAA 
CATAGATCTC TCTCTCCTCG TCAAAGCAAT CTACGAAATC AACCGCAAAT GACTCGCAGT 
AATTCCTCGA GCGGTGTTGT CAATACCTAC CAACCTCCGA ATTCCCCTCG TGGCCGAGTA 
GTTACTGTAA GAAAGAACAA TCAGTCTGAT GGTGTTGGTC TGAGAACATT TCGAGTTTCA 
AGTAACTCGA ACTTCAGAAC TGCAAGTCCA TCAAGATCCG TTCAACCATC TTTAGCGGAT 
CTAGAGTTTC CGCCCCCACC CGCAGATTTG CCTCCTCCCC CTGACGAAAC TTTCTCTGGT 
CAAGAACAAG CTGAACTTCC GCCTCCCGTT TCTTGTACCG AGCTATCTCA GATAAGAAAT 
TCTCCTCTTT CTATAAGAAA AGCAAAGAGC ACGGATTGGC GCACGAAAGA AGAAGATAAT 
GAACAGGAAG ACAGGAACGA CGTTAGTAAC ATAGAGCCTT CCGTCAAAGA AGCTTGTTCG 
AGGTTTGGAG TTAATTTAAG GCGCAGAGAA ACTCAAGATA ATTCTTGTAG AAACTCTGAT 
AATAAAAGAA CGGGATTTAA ATCCAGAATG GAGACAGTAG AGCCTGCTGC ACCGCCAGAG 
GAAGCACCAC CTCCCCCACC GCCACCTCCG CCGCCACTTT CTACGAATAC ACCCCCCGAT 
AGCTTCGAAC GTAAACCAGG CATGAAAGAA ATGTTAGAAT TAAAATTGAT CAATGAAATT 
AAACAGAGCG CGGAAACAAA ACATGGTACG ACTACGAAAA AGACTGGAGC GACAAGCAAT 
ACTCCATCCG CACCTTTAGA TCCAGCGTCG CAGCTACTCT CGGAACTTTG TGCTAGTTTT 
AATATGGAGT CTGGACAGAG ACACGCCCAA AACGAGTACG CTGTATCAAC TTTAAAAACC 
AATGACGTAA ATCAAGAACA ACAACAGCAA CAAAGTCATA ACGCTCATAA GGATTCGTCG 
GTGTCCTCTC CAGTGACCGA GTCTCTCCTG TCCAGTGGAA ATGTTGGATT CAAGCTGAAA 
AGAGTTGACA AGCGGAATAA TCCGCAAAAA GAAGAAACAT CCGATGGACA AATAATTGAT 
TTTAAAGCGA GACTTCGAAA AGTTGAGAAC GCAGAAAAAG AAAAATCCTT GGAAGAGAAA 
AGTACCGTCA CTGAACAGTC TTCAGAATCA GAAGAGCAAC AAGATGACAA ACGTAGAAGC 
ACTGGTAGTA TCAGTAGTTT AAAGAAGCTT TGGGAGAACA AAGAATCTTG TGACAGTCAG 
CCTCATAGTC CAAAACTCAA TGTCAGAGGA AGTGGTGGTA AACAAGAAAT GATAGACCAG 
ACAGAAGACT CACCGGAGGA TCATAGTGGA GCTTCGACAC GTAGTCAGAG TTCCGGTAGC 
AAAAGTGACG CTAGACTATG GCCACCGCCC GAACCTGAAA AACCGGCAGT TCCAGCAAAG 
CCATTAAAAC CACTATGTTC ATCAACGAAG CACTTTGGTT CTTCGATTTA CGCAACGCCA 
AACTGTACGA AATCTCAGCA CGCTGAGGAC GACCTAAACA AACAGAATAC AGATTCAAAG 
ACTGCGAAAC AGGCAGTATT GGAGCTTTCG ACGTTGATTG AGAGCAGCAT ATTAAACTTG 
AAAAGTAGTC CAACAATTGT AATGACAAGT TGGCTTCAGC TCTCTGACAA GGTAGGGCTA 
CTACACGGAA TGTGCACGAA TCTCGCAGAC AGTGGTATCG CACCACATGC GCGGTTTCAA 
TTTCGCGAAC TTCTTGCAAG ATTAGAACTT CAGGCACGGC AGCTTCGTGC AGCCGGTATT 
CGGAACGTTG CAGAGAATAC AAGGCTTCTC ACTGATCTTC AAAACACGAT AAAGGATGTT 
GTTAATGCTG TGCAAAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012141824.1
CDS	278..4618
Translation	MGAQQTKERIVPAGSTARQTRKQPRNLKESRLVGSNIFTEHSALLQSRPLPHIPALPDGDPPSGSAIQPISQQVNIQQHAGVSSTGLLEAANRWTSKENLLAQEEDDPQLFVALYDFQAGGENQLSLKKGEQVRILSYNKSGEWCEAHSSTGQVGWVPSNYVTPVNSLEKHSWYHGRISRNAAEYLLSSGINGSFLVRESESSPGQRSISLRYEGRVYHYRINEDSEGKMFVTTESKFNTLAELVHHHSMLADGLITQLLYPAPKHNKPTVFPLSPEPDEWEINRTDIVMRHKLGGGQYGDVYEAVWKRYNMTVAVKTLKEDTMALKDFLEEAAIMKEMKHRNLVQLLGVCTREPPFYIITEFMSKGNLLDYLRNESKHQINAVVLMHMATQIASGMSYLESRNFIHRDLAARNCLVGENHLVKVADFGLARLMRDDTYTAHAGAKFPIKWTAPEGLAYNKFSTKSDVWAFGILLWEIATYGMSPYPGVDLTDVYHMLEKGYRMECPPGCPPKVYELMRQCWQWAAADRPTFKEIHHSLENMFQESSITEEVEKQLQGGGDIPLLSYKKSQTGSTGNIHGLVLVSEPLPSSETTSSVTKLSTFTGGLSSKNNSNIVQMRRSTNKKGKQAPAPPKRTSLLSSCSSFRDSAYQEQDTQNTETNTMTLDDATDLNGIDKILEGIARDLATMAQGTIAAGGCEVEEDGEGSQGTAEPNFIPQPSTSPEPIPGLVACSQKQIKPRPYPSKEPLPQKLVQVGALEVQNVKRAINRYGTLPKGARIGAYLESLRQSGMPSNQESTTVVSSIAPSSVEQHETSMDNSQHRSLSPRQSNLRNQPQMTRSNSSSGVVNTYQPPNSPRGRVVTVRKNNQSDGVGLRTFRVSSNSNFRTASPSRSVQPSLADLEFPPPPADLPPPPDETFSGQEQAELPPPVSCTELSQIRNSPLSIRKAKSTDWRTKEEDNEQEDRNDVSNIEPSVKEACSRFGVNLRRRETQDNSCRNSDNKRTGFKSRMETVEPAAPPEEAPPPPPPPPPPLSTNTPPDSFERKPGMKEMLELKLINEIKQSAETKHGTTTKKTGATSNTPSAPLDPASQLLSELCASFNMESGQRHAQNEYAVSTLKTNDVNQEQQQQQSHNAHKDSSVSSPVTESLLSSGNVGFKLKRVDKRNNPQKEETSDGQIIDFKARLRKVENAEKEKSLEEKSTVTEQSSESEEQQDDKRRSTGSISSLKKLWENKESCDSQPHSPKLNVRGSGGKQEMIDQTEDSPEDHSGASTRSQSSGSKSDARLWPPPEPEKPAVPAKPLKPLCSSTKHFGSSIYATPNCTKSQHAEDDLNKQNTDSKTAKQAVLELSTLIESSILNLKSSPTIVMTSWLQLSDKVGLLHGMCTNLADSGIAPHARFQFRELLARLELQARQLRAAGIRNVAENTRLLTDLQNTIKDVVNAVQR

Target ORF information:

RefSeq Version	XM_012286434.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata tyrosine-protein kinase Abl (LOC100878636), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012286434.1

ORF Insert Sequence:

ATGGGTGCTC AACAGACTAA GGAAAGAATT GTTCCTGCCG GCTCTACTGC GCGACAGACT 
CGCAAACAGC CAAGGAACCT CAAGGAGTCT CGTCTTGTTG GTTCCAATAT ATTTACAGAA 
CACAGCGCTC TCTTACAAAG CAGACCGCTA CCTCACATTC CGGCATTACC AGATGGCGAT 
CCTCCTAGTG GCTCTGCTAT CCAACCAATT TCACAACAAG TGAATATTCA ACAACATGCT 
GGTGTTTCAT CTACAGGGCT TTTGGAAGCT GCAAACAGGT GGACAAGTAA AGAAAACTTA 
TTAGCACAGG AGGAAGATGA TCCACAACTA TTTGTGGCTC TATATGATTT TCAAGCAGGT 
GGAGAAAACC AACTTAGTCT TAAAAAGGGA GAGCAAGTTC GTATTCTAAG TTATAATAAA 
AGTGGAGAAT GGTGTGAAGC TCATTCAAGT ACTGGTCAAG TGGGGTGGGT TCCTTCAAAT 
TATGTCACCC CTGTAAATTC TTTGGAAAAA CATTCTTGGT ATCATGGAAG GATATCCAGA 
AATGCTGCTG AATATCTCCT AAGTTCTGGT ATAAATGGTA GCTTTCTGGT TCGTGAATCA 
GAAAGCAGTC CAGGTCAACG CAGTATTTCT TTGAGATATG AGGGTAGAGT CTATCATTAT 
AGGATTAATG AGGATAGTGA AGGAAAGATG TTTGTCACAA CAGAAAGCAA ATTTAATACT 
CTGGCAGAAT TGGTACATCA TCATTCTATG CTTGCTGATG GTTTAATCAC ACAGCTACTT 
TATCCTGCAC CAAAGCATAA CAAACCAACT GTTTTTCCAC TTAGTCCAGA ACCAGATGAA 
TGGGAGATTA ATAGGACAGA TATAGTTATG AGGCATAAGT TAGGTGGAGG ACAATATGGA 
GATGTCTATG AAGCAGTATG GAAAAGATAT AACATGACTG TGGCTGTAAA AACATTAAAA 
GAAGACACAA TGGCCCTAAA GGACTTTTTG GAGGAAGCTG CAATCATGAA GGAGATGAAG 
CATAGGAATT TAGTTCAGCT ACTGGGTGTA TGTACTCGAG AACCACCTTT CTACATCATT 
ACAGAGTTTA TGAGCAAAGG AAATCTGCTA GACTATTTAC GGAACGAGAG TAAACATCAA 
ATCAATGCTG TCGTTTTGAT GCACATGGCC ACACAGATTG CTAGTGGAAT GAGTTATTTG 
GAGAGTAGAA ATTTTATACA CAGAGATCTA GCAGCTCGAA ACTGCCTAGT GGGTGAAAAC 
CATCTGGTAA AGGTCGCAGA CTTTGGCTTG GCCCGGTTGA TGAGAGATGA TACGTATACG 
GCTCACGCTG GAGCAAAGTT TCCAATAAAA TGGACTGCTC CAGAAGGATT AGCTTACAAT 
AAATTCTCTA CGAAGTCTGA TGTGTGGGCA TTTGGAATTT TACTTTGGGA GATAGCAACC 
TATGGCATGT CCCCATACCC TGGTGTTGAT TTGACGGATG TGTATCACAT GTTGGAGAAA 
GGCTACCGAA TGGAATGTCC ACCTGGATGT CCACCGAAGG TATATGAACT GATGCGTCAA 
TGCTGGCAGT GGGCTGCTGC TGACCGGCCT ACCTTCAAAG AGATTCATCA TTCTCTTGAA 
AATATGTTCC AAGAATCCAG TATTACCGAG GAAGTTGAAA AACAACTGCA AGGTGGCGGC 
GATATCCCTT TACTTTCATA TAAAAAATCT CAAACTGGTA GTACTGGAAA CATACATGGG 
CTTGTTCTAG TCTCCGAGCC ATTACCCTCT TCAGAAACTA CTAGCTCGGT AACAAAACTG 
AGTACGTTTA CGGGTGGTCT ATCGAGTAAA AATAACAGCA ATATCGTCCA AATGAGGCGT 
TCTACGAATA AGAAAGGAAA ACAAGCTCCG GCACCTCCAA AGAGAACGAG CCTGCTGTCG 
TCGTGCAGTT CGTTTCGCGA TTCTGCCTAC CAAGAGCAAG ATACCCAAAA TACTGAAACA 
AACACCATGA CTCTCGACGA TGCCACGGAT CTAAATGGTA TTGATAAGAT TCTCGAAGGT 
ATTGCGCGAG ATCTCGCGAC GATGGCTCAA GGAACGATTG CTGCAGGAGG CTGCGAAGTC 
GAGGAGGATG GAGAGGGTTC TCAAGGTACG GCGGAACCAA ATTTTATCCC TCAACCTTCG 
ACGTCCCCGG AGCCTATACC TGGACTAGTA GCCTGTTCTC AGAAACAAAT CAAACCACGA 
CCTTATCCAT CAAAGGAACC GTTACCACAG AAGCTGGTAC AAGTGGGAGC ATTAGAAGTA 
CAGAACGTAA AAAGAGCCAT TAATCGTTAT GGCACACTAC CAAAAGGTGC TAGGATCGGA 
GCATACCTAG AATCTTTGCG TCAAAGTGGT ATGCCATCGA ATCAAGAATC TACAACGGTG 
GTTTCTTCCA TTGCACCTAG TTCAGTAGAA CAACATGAGA CTTCTATGGA CAATTCGCAA 
CATAGATCTC TCTCTCCTCG TCAAAGCAAT CTACGAAATC AACCGCAAAT GACTCGCAGT 
AATTCCTCGA GCGGTGTTGT CAATACCTAC CAACCTCCGA ATTCCCCTCG TGGCCGAGTA 
GTTACTGTAA GAAAGAACAA TCAGTCTGAT GGTGTTGGTC TGAGAACATT TCGAGTTTCA 
AGTAACTCGA ACTTCAGAAC TGCAAGTCCA TCAAGATCCG TTCAACCATC TTTAGCGGAT 
CTAGAGTTTC CGCCCCCACC CGCAGATTTG CCTCCTCCCC CTGACGAAAC TTTCTCTGGT 
CAAGAACAAG CTGAACTTCC GCCTCCCGTT TCTTGTACCG AGCTATCTCA GATAAGAAAT 
TCTCCTCTTT CTATAAGAAA AGCAAAGAGC ACGGATTGGC GCACGAAAGA AGAAGATAAT 
GAACAGGAAG ACAGGAACGA CGTTAGTAAC ATAGAGCCTT CCGTCAAAGA AGCTTGTTCG 
AGGTTTGGAG TTAATTTAAG GCGCAGAGAA ACTCAAGATA ATTCTTGTAG AAACTCTGAT 
AATAAAAGAA CGGGATTTAA ATCCAGAATG GAGACAGTAG AGCCTGCTGC ACCGCCAGAG 
GAAGCACCAC CTCCCCCACC GCCACCTCCG CCGCCACTTT CTACGAATAC ACCCCCCGAT 
AGCTTCGAAC GTAAACCAGG CATGAAAGAA ATGTTAGAAT TAAAATTGAT CAATGAAATT 
AAACAGAGCG CGGAAACAAA ACATGGTACG ACTACGAAAA AGACTGGAGC GACAAGCAAT 
ACTCCATCCG CACCTTTAGA TCCAGCGTCG CAGCTACTCT CGGAACTTTG TGCTAGTTTT 
AATATGGAGT CTGGACAGAG ACACGCCCAA AACGAGTACG CTGTATCAAC TTTAAAAACC 
AATGACGTAA ATCAAGAACA ACAACAGCAA CAAAGTCATA ACGCTCATAA GGATTCGTCG 
GTGTCCTCTC CAGTGACCGA GTCTCTCCTG TCCAGTGGAA ATGTTGGATT CAAGCTGAAA 
AGAGTTGACA AGCGGAATAA TCCGCAAAAA GAAGAAACAT CCGATGGACA AATAATTGAT 
TTTAAAGCGA GACTTCGAAA AGTTGAGAAC GCAGAAAAAG AAAAATCCTT GGAAGAGAAA 
AGTACCGTCA CTGAACAGTC TTCAGAATCA GAAGAGCAAC AAGATGACAA ACGTAGAAGC 
ACTGGTAGTA TCAGTAGTTT AAAGAAGCTT TGGGAGAACA AAGAATCTTG TGACAGTCAG 
CCTCATAGTC CAAAACTCAA TGTCAGAGGA AGTGGTGGTA AACAAGAAAT GATAGACCAG 
ACAGAAGACT CACCGGAGGA TCATAGTGGA GCTTCGACAC GTAGTCAGAG TTCCGGTAGC 
AAAAGTGACG CTAGACTATG GCCACCGCCC GAACCTGAAA AACCGGCAGT TCCAGCAAAG 
CCATTAAAAC CACTATGTTC ATCAACGAAG CACTTTGGTT CTTCGATTTA CGCAACGCCA 
AACTGTACGA AATCTCAGCA CGCTGAGGAC GACCTAAACA AACAGAATAC AGATTCAAAG 
ACTGCGAAAC AGGCAGTATT GGAGCTTTCG ACGTTGATTG AGAGCAGCAT ATTAAACTTG 
AAAAGTAGTC CAACAATTGT AATGACAAGT TGGCTTCAGC TCTCTGACAA GGTAGGGCTA 
CTACACGGAA TGTGCACGAA TCTCGCAGAC AGTGGTATCG CACCACATGC GCGGTTTCAA 
TTTCGCGAAC TTCTTGCAAG ATTAGAACTT CAGGCACGGC AGCTTCGTGC AGCCGGTATT 
CGGAACGTTG CAGAGAATAC AAGGCTTCTC ACTGATCTTC AAAACACGAT AAAGGATGTT 
GTTAATGCTG TGCAAAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100878636 ( XM_012286435.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012141825.1 Megachile rotundata tyrosine-protein kinase Abl (LOC100878636), transcript variant X3, mRNA.

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CloneID	OMe105644
Clone ID Related Accession (Same CDS sequence)	XM_012286435.1
Accession Version	XM_012286435.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	tyrosine-protein kinase Abl isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797180.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGCTC AACAGACTAA GGAAAGAATT GTTCCTGCCG GCTCTACTGC GCGACAGACT 
CGCAAACAGC CAAGGAACCT CAAGGAGTCT CGTCTTGTTG GTTCCAATAT ATTTACAGAA 
CACAGCGAAG CTCTCTTACA AAGCAGACCG CTACCTCACA TTCCGGCATT ACCAGATGGC 
GATCCTCCTA GTGGCTCTGC TATCCAACCA ATTTCACAAC AAGTGAATAT TCAACAACAT 
GCTGGTGTTT CATCTACAGG GCTTTTGGAA GCTGCAAACA GGTGGACAAG TAAAGAAAAC 
TTATTAGCAC AGGAGGAAGA TGATCCACAA CTATTTGTGG CTCTATATGA TTTTCAAGCA 
GGTGGAGAAA ACCAACTTAG TCTTAAAAAG GGAGAGCAAG TTCGTATTCT AAGTTATAAT 
AAAAGTGGAG AATGGTGTGA AGCTCATTCA AGTACTGGTC AAGTGGGGTG GGTTCCTTCA 
AATTATGTCA CCCCTGTAAA TTCTTTGGAA AAACATTCTT GGTATCATGG AAGGATATCC 
AGAAATGCTG CTGAATATCT CCTAAGTTCT GGTATAAATG GTAGCTTTCT GGTTCGTGAA 
TCAGAAAGCA GTCCAGGTCA ACGCAGTATT TCTTTGAGAT ATGAGGGTAG AGTCTATCAT 
TATAGGATTA ATGAGGATAG TGAAGGAAAG ATGTTTGTCA CAACAGAAAG CAAATTTAAT 
ACTCTGGCAG AATTGGTACA TCATCATTCT ATGCTTGCTG ATGGTTTAAT CACACAGCTA 
CTTTATCCTG CACCAAAGCA TAACAAACCA ACTGTTTTTC CACTTAGTCC AGAACCAGAT 
GAATGGGAGA TTAATAGGAC AGATATAGTT ATGAGGCATA AGTTAGGTGG AGGACAATAT 
GGAGATGTCT ATGAAGCAGT ATGGAAAAGA TATAACATGA CTGTGGCTGT AAAAACATTA 
AAAGAAGACA CAATGGCCCT AAAGGACTTT TTGGAGGAAG CTGCAATCAT GAAGGAGATG 
AAGCATAGGA ATTTAGTTCA GCTACTGGGT GTATGTACTC GAGAACCACC TTTCTACATC 
ATTACAGAGT TTATGAGCAA AGGAAATCTG CTAGACTATT TACGGAACGA GAGTAAACAT 
CAAATCAATG CTGTCGTTTT GATGCACATG GCCACACAGA TTGCTAGTGG AATGAGTTAT 
TTGGAGAGTA GAAATTTTAT ACACAGAGAT CTAGCAGCTC GAAACTGCCT AGTGGGTGAA 
AACCATCTGG TAAAGGTCGC AGACTTTGGC TTGGCCCGGT TGATGAGAGA TGATACGTAT 
ACGGCTCACG CTGGAGCAAA GTTTCCAATA AAATGGACTG CTCCAGAAGG ATTAGCTTAC 
AATAAATTCT CTACGAAGTC TGATGTGTGG GCATTTGGAA TTTTACTTTG GGAGATAGCA 
ACCTATGGCA TGTCCCCATA CCCTGGTGTT GATTTGACGG ATGTGTATCA CATGTTGGAG 
AAAGGCTACC GAATGGAATG TCCACCTGGA TGTCCACCGA AGGTATATGA ACTGATGCGT 
CAATGCTGGC AGTGGGCTGC TGCTGACCGG CCTACCTTCA AAGAGATTCA TCATTCTCTT 
GAAAATATGT TCCAAGAATC CAGTATTACC GAGGAAGTTG AAAAACAACT GCAAGGTGGC 
GGCGATATCC CTTTACTTTC ATATAAAAAA TCTCAAACTG GTAGTACTGG AAACATACAT 
GGGCTTGTTC TAGTCTCCGA GCCATTACCC TCTTCAGAAA CTACTAGCTC GGTAACAAAA 
CTGAGTACGT TTACGGGTGG TCTATCGAGT AAAAATAACA GCAATATCGT CCAAATGAGG 
CGTTCTACGA ATAAGAAAGG AAAACAAGCT CCGGCACCTC CAAAGAGAAC GAGTTCGTTT 
CGCGATTCTG CCTACCAAGA GCAAGATACC CAAAATACTG AAACAAACAC CATGACTCTC 
GACGATGCCA CGGATCTAAA TGGTATTGAT AAGATTCTCG AAGGTATTGC GCGAGATCTC 
GCGACGATGG CTCAAGGAAC GATTGCTGCA GGAGGCTGCG AAGTCGAGGA GGATGGAGAG 
GGTTCTCAAG GTACGGCGGA ACCAAATTTT ATCCCTCAAC CTTCGACGTC CCCGGAGCCT 
ATACCTGGAC TAGTAGCCTG TTCTCAGAAA CAAATCAAAC CACGACCTTA TCCATCAAAG 
GAACCGTTAC CACAGAAGCT GGTACAAGTG GGAGCATTAG AAGTACAGAA CGTAAAAAGA 
GCCATTAATC GTTATGGCAC ACTACCAAAA GGTGCTAGGA TCGGAGCATA CCTAGAATCT 
TTGCGTCAAA GTGGTATGCC ATCGAATCAA GAATCTACAA CGGTGGTTTC TTCCATTGCA 
CCTAGTTCAG TAGAACAACA TGAGACTTCT ATGGACAATT CGCAACATAG ATCTCTCTCT 
CCTCGTCAAA GCAATCTACG AAATCAACCG CAAATGACTC GCAGTAATTC CTCGAGCGGT 
GTTGTCAATA CCTACCAACC TCCGAATTCC CCTCGTGGCC GAGTAGTTAC TGTAAGAAAG 
AACAATCAGT CTGATGGTGT TGGTCTGAGA ACATTTCGAG TTTCAAGTAA CTCGAACTTC 
AGAACTGCAA GTCCATCAAG ATCCGTTCAA CCATCTTTAG CGGATCTAGA GTTTCCGCCC 
CCACCCGCAG ATTTGCCTCC TCCCCCTGAC GAAACTTTCT CTGGTCAAGA ACAAGCTGAA 
CTTCCGCCTC CCGTTTCTTG TACCGAGCTA TCTCAGATAA GAAATTCTCC TCTTTCTATA 
AGAAAAGCAA AGAGCACGGA TTGGCGCACG AAAGAAGAAG ATAATGAACA GGAAGACAGG 
AACGACGTTA GTAACATAGA GCCTTCCGTC AAAGAAGCTT GTTCGAGGTT TGGAGTTAAT 
TTAAGGCGCA GAGAAACTCA AGATAATTCT TGTAGAAACT CTGATAATAA AAGAACGGGA 
TTTAAATCCA GAATGGAGAC AGTAGAGCCT GCTGCACCGC CAGAGGAAGC ACCACCTCCC 
CCACCGCCAC CTCCGCCGCC ACTTTCTACG AATACACCCC CCGATAGCTT CGAACGTAAA 
CCAGGCATGA AAGAAATGTT AGAATTAAAA TTGATCAATG AAATTAAACA GAGCGCGGAA 
ACAAAACATG GTACGACTAC GAAAAAGACT GGAGCGACAA GCAATACTCC ATCCGCACCT 
TTAGATCCAG CGTCGCAGCT ACTCTCGGAA CTTTGTGCTA GTTTTAATAT GGAGTCTGGA 
CAGAGACACG CCCAAAACGA GTACGCTGTA TCAACTTTAA AAACCAATGA CGTAAATCAA 
GAACAACAAC AGCAACAAAG TCATAACGCT CATAAGGATT CGTCGGTGTC CTCTCCAGTG 
ACCGAGTCTC TCCTGTCCAG TGGAAATGTT GGATTCAAGC TGAAAAGAGT TGACAAGCGG 
AATAATCCGC AAAAAGAAGA AACATCCGAT GGACAAATAA TTGATTTTAA AGCGAGACTT 
CGAAAAGTTG AGAACGCAGA AAAAGAAAAA TCCTTGGAAG AGAAAAGTAC CGTCACTGAA 
CAGTCTTCAG AATCAGAAGA GCAACAAGAT GACAAACGTA GAAGCACTGG TAGTATCAGT 
AGTTTAAAGA AGCTTTGGGA GAACAAAGAA TCTTGTGACA GTCAGCCTCA TAGTCCAAAA 
CTCAATGTCA GAGGAAGTGG TGGTAAACAA GAAATGATAG ACCAGACAGA AGACTCACCG 
GAGGATCATA GTGGAGCTTC GACACGTAGT CAGAGTTCCG GTAGCAAAAG TGACGCTAGA 
CTATGGCCAC CGCCCGAACC TGAAAAACCG GCAGTTCCAG CAAAGCCATT AAAACCACTA 
TGTTCATCAA CGAAGCACTT TGGTTCTTCG ATTTACGCAA CGCCAAACTG TACGAAATCT 
CAGCACGCTG AGGACGACCT AAACAAACAG AATACAGATT CAAAGACTGC GAAACAGGCA 
GTATTGGAGC TTTCGACGTT GATTGAGAGC AGCATATTAA ACTTGAAAAG TAGTCCAACA 
ATTGTAATGA CAAGTTGGCT TCAGCTCTCT GACAAGGTAG GGCTACTACA CGGAATGTGC 
ACGAATCTCG CAGACAGTGG TATCGCACCA CATGCGCGGT TTCAATTTCG CGAACTTCTT 
GCAAGATTAG AACTTCAGGC ACGGCAGCTT CGTGCAGCCG GTATTCGGAA CGTTGCAGAG 
AATACAAGGC TTCTCACTGA TCTTCAAAAC ACGATAAAGG ATGTTGTTAA TGCTGTGCAA 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012141825.1
CDS	278..4603
Translation	MGAQQTKERIVPAGSTARQTRKQPRNLKESRLVGSNIFTEHSEALLQSRPLPHIPALPDGDPPSGSAIQPISQQVNIQQHAGVSSTGLLEAANRWTSKENLLAQEEDDPQLFVALYDFQAGGENQLSLKKGEQVRILSYNKSGEWCEAHSSTGQVGWVPSNYVTPVNSLEKHSWYHGRISRNAAEYLLSSGINGSFLVRESESSPGQRSISLRYEGRVYHYRINEDSEGKMFVTTESKFNTLAELVHHHSMLADGLITQLLYPAPKHNKPTVFPLSPEPDEWEINRTDIVMRHKLGGGQYGDVYEAVWKRYNMTVAVKTLKEDTMALKDFLEEAAIMKEMKHRNLVQLLGVCTREPPFYIITEFMSKGNLLDYLRNESKHQINAVVLMHMATQIASGMSYLESRNFIHRDLAARNCLVGENHLVKVADFGLARLMRDDTYTAHAGAKFPIKWTAPEGLAYNKFSTKSDVWAFGILLWEIATYGMSPYPGVDLTDVYHMLEKGYRMECPPGCPPKVYELMRQCWQWAAADRPTFKEIHHSLENMFQESSITEEVEKQLQGGGDIPLLSYKKSQTGSTGNIHGLVLVSEPLPSSETTSSVTKLSTFTGGLSSKNNSNIVQMRRSTNKKGKQAPAPPKRTSSFRDSAYQEQDTQNTETNTMTLDDATDLNGIDKILEGIARDLATMAQGTIAAGGCEVEEDGEGSQGTAEPNFIPQPSTSPEPIPGLVACSQKQIKPRPYPSKEPLPQKLVQVGALEVQNVKRAINRYGTLPKGARIGAYLESLRQSGMPSNQESTTVVSSIAPSSVEQHETSMDNSQHRSLSPRQSNLRNQPQMTRSNSSSGVVNTYQPPNSPRGRVVTVRKNNQSDGVGLRTFRVSSNSNFRTASPSRSVQPSLADLEFPPPPADLPPPPDETFSGQEQAELPPPVSCTELSQIRNSPLSIRKAKSTDWRTKEEDNEQEDRNDVSNIEPSVKEACSRFGVNLRRRETQDNSCRNSDNKRTGFKSRMETVEPAAPPEEAPPPPPPPPPPLSTNTPPDSFERKPGMKEMLELKLINEIKQSAETKHGTTTKKTGATSNTPSAPLDPASQLLSELCASFNMESGQRHAQNEYAVSTLKTNDVNQEQQQQQSHNAHKDSSVSSPVTESLLSSGNVGFKLKRVDKRNNPQKEETSDGQIIDFKARLRKVENAEKEKSLEEKSTVTEQSSESEEQQDDKRRSTGSISSLKKLWENKESCDSQPHSPKLNVRGSGGKQEMIDQTEDSPEDHSGASTRSQSSGSKSDARLWPPPEPEKPAVPAKPLKPLCSSTKHFGSSIYATPNCTKSQHAEDDLNKQNTDSKTAKQAVLELSTLIESSILNLKSSPTIVMTSWLQLSDKVGLLHGMCTNLADSGIAPHARFQFRELLARLELQARQLRAAGIRNVAENTRLLTDLQNTIKDVVNAVQR

Target ORF information:

RefSeq Version	XM_012286435.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata tyrosine-protein kinase Abl (LOC100878636), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012286435.1

ORF Insert Sequence:

ATGGGTGCTC AACAGACTAA GGAAAGAATT GTTCCTGCCG GCTCTACTGC GCGACAGACT 
CGCAAACAGC CAAGGAACCT CAAGGAGTCT CGTCTTGTTG GTTCCAATAT ATTTACAGAA 
CACAGCGAAG CTCTCTTACA AAGCAGACCG CTACCTCACA TTCCGGCATT ACCAGATGGC 
GATCCTCCTA GTGGCTCTGC TATCCAACCA ATTTCACAAC AAGTGAATAT TCAACAACAT 
GCTGGTGTTT CATCTACAGG GCTTTTGGAA GCTGCAAACA GGTGGACAAG TAAAGAAAAC 
TTATTAGCAC AGGAGGAAGA TGATCCACAA CTATTTGTGG CTCTATATGA TTTTCAAGCA 
GGTGGAGAAA ACCAACTTAG TCTTAAAAAG GGAGAGCAAG TTCGTATTCT AAGTTATAAT 
AAAAGTGGAG AATGGTGTGA AGCTCATTCA AGTACTGGTC AAGTGGGGTG GGTTCCTTCA 
AATTATGTCA CCCCTGTAAA TTCTTTGGAA AAACATTCTT GGTATCATGG AAGGATATCC 
AGAAATGCTG CTGAATATCT CCTAAGTTCT GGTATAAATG GTAGCTTTCT GGTTCGTGAA 
TCAGAAAGCA GTCCAGGTCA ACGCAGTATT TCTTTGAGAT ATGAGGGTAG AGTCTATCAT 
TATAGGATTA ATGAGGATAG TGAAGGAAAG ATGTTTGTCA CAACAGAAAG CAAATTTAAT 
ACTCTGGCAG AATTGGTACA TCATCATTCT ATGCTTGCTG ATGGTTTAAT CACACAGCTA 
CTTTATCCTG CACCAAAGCA TAACAAACCA ACTGTTTTTC CACTTAGTCC AGAACCAGAT 
GAATGGGAGA TTAATAGGAC AGATATAGTT ATGAGGCATA AGTTAGGTGG AGGACAATAT 
GGAGATGTCT ATGAAGCAGT ATGGAAAAGA TATAACATGA CTGTGGCTGT AAAAACATTA 
AAAGAAGACA CAATGGCCCT AAAGGACTTT TTGGAGGAAG CTGCAATCAT GAAGGAGATG 
AAGCATAGGA ATTTAGTTCA GCTACTGGGT GTATGTACTC GAGAACCACC TTTCTACATC 
ATTACAGAGT TTATGAGCAA AGGAAATCTG CTAGACTATT TACGGAACGA GAGTAAACAT 
CAAATCAATG CTGTCGTTTT GATGCACATG GCCACACAGA TTGCTAGTGG AATGAGTTAT 
TTGGAGAGTA GAAATTTTAT ACACAGAGAT CTAGCAGCTC GAAACTGCCT AGTGGGTGAA 
AACCATCTGG TAAAGGTCGC AGACTTTGGC TTGGCCCGGT TGATGAGAGA TGATACGTAT 
ACGGCTCACG CTGGAGCAAA GTTTCCAATA AAATGGACTG CTCCAGAAGG ATTAGCTTAC 
AATAAATTCT CTACGAAGTC TGATGTGTGG GCATTTGGAA TTTTACTTTG GGAGATAGCA 
ACCTATGGCA TGTCCCCATA CCCTGGTGTT GATTTGACGG ATGTGTATCA CATGTTGGAG 
AAAGGCTACC GAATGGAATG TCCACCTGGA TGTCCACCGA AGGTATATGA ACTGATGCGT 
CAATGCTGGC AGTGGGCTGC TGCTGACCGG CCTACCTTCA AAGAGATTCA TCATTCTCTT 
GAAAATATGT TCCAAGAATC CAGTATTACC GAGGAAGTTG AAAAACAACT GCAAGGTGGC 
GGCGATATCC CTTTACTTTC ATATAAAAAA TCTCAAACTG GTAGTACTGG AAACATACAT 
GGGCTTGTTC TAGTCTCCGA GCCATTACCC TCTTCAGAAA CTACTAGCTC GGTAACAAAA 
CTGAGTACGT TTACGGGTGG TCTATCGAGT AAAAATAACA GCAATATCGT CCAAATGAGG 
CGTTCTACGA ATAAGAAAGG AAAACAAGCT CCGGCACCTC CAAAGAGAAC GAGTTCGTTT 
CGCGATTCTG CCTACCAAGA GCAAGATACC CAAAATACTG AAACAAACAC CATGACTCTC 
GACGATGCCA CGGATCTAAA TGGTATTGAT AAGATTCTCG AAGGTATTGC GCGAGATCTC 
GCGACGATGG CTCAAGGAAC GATTGCTGCA GGAGGCTGCG AAGTCGAGGA GGATGGAGAG 
GGTTCTCAAG GTACGGCGGA ACCAAATTTT ATCCCTCAAC CTTCGACGTC CCCGGAGCCT 
ATACCTGGAC TAGTAGCCTG TTCTCAGAAA CAAATCAAAC CACGACCTTA TCCATCAAAG 
GAACCGTTAC CACAGAAGCT GGTACAAGTG GGAGCATTAG AAGTACAGAA CGTAAAAAGA 
GCCATTAATC GTTATGGCAC ACTACCAAAA GGTGCTAGGA TCGGAGCATA CCTAGAATCT 
TTGCGTCAAA GTGGTATGCC ATCGAATCAA GAATCTACAA CGGTGGTTTC TTCCATTGCA 
CCTAGTTCAG TAGAACAACA TGAGACTTCT ATGGACAATT CGCAACATAG ATCTCTCTCT 
CCTCGTCAAA GCAATCTACG AAATCAACCG CAAATGACTC GCAGTAATTC CTCGAGCGGT 
GTTGTCAATA CCTACCAACC TCCGAATTCC CCTCGTGGCC GAGTAGTTAC TGTAAGAAAG 
AACAATCAGT CTGATGGTGT TGGTCTGAGA ACATTTCGAG TTTCAAGTAA CTCGAACTTC 
AGAACTGCAA GTCCATCAAG ATCCGTTCAA CCATCTTTAG CGGATCTAGA GTTTCCGCCC 
CCACCCGCAG ATTTGCCTCC TCCCCCTGAC GAAACTTTCT CTGGTCAAGA ACAAGCTGAA 
CTTCCGCCTC CCGTTTCTTG TACCGAGCTA TCTCAGATAA GAAATTCTCC TCTTTCTATA 
AGAAAAGCAA AGAGCACGGA TTGGCGCACG AAAGAAGAAG ATAATGAACA GGAAGACAGG 
AACGACGTTA GTAACATAGA GCCTTCCGTC AAAGAAGCTT GTTCGAGGTT TGGAGTTAAT 
TTAAGGCGCA GAGAAACTCA AGATAATTCT TGTAGAAACT CTGATAATAA AAGAACGGGA 
TTTAAATCCA GAATGGAGAC AGTAGAGCCT GCTGCACCGC CAGAGGAAGC ACCACCTCCC 
CCACCGCCAC CTCCGCCGCC ACTTTCTACG AATACACCCC CCGATAGCTT CGAACGTAAA 
CCAGGCATGA AAGAAATGTT AGAATTAAAA TTGATCAATG AAATTAAACA GAGCGCGGAA 
ACAAAACATG GTACGACTAC GAAAAAGACT GGAGCGACAA GCAATACTCC ATCCGCACCT 
TTAGATCCAG CGTCGCAGCT ACTCTCGGAA CTTTGTGCTA GTTTTAATAT GGAGTCTGGA 
CAGAGACACG CCCAAAACGA GTACGCTGTA TCAACTTTAA AAACCAATGA CGTAAATCAA 
GAACAACAAC AGCAACAAAG TCATAACGCT CATAAGGATT CGTCGGTGTC CTCTCCAGTG 
ACCGAGTCTC TCCTGTCCAG TGGAAATGTT GGATTCAAGC TGAAAAGAGT TGACAAGCGG 
AATAATCCGC AAAAAGAAGA AACATCCGAT GGACAAATAA TTGATTTTAA AGCGAGACTT 
CGAAAAGTTG AGAACGCAGA AAAAGAAAAA TCCTTGGAAG AGAAAAGTAC CGTCACTGAA 
CAGTCTTCAG AATCAGAAGA GCAACAAGAT GACAAACGTA GAAGCACTGG TAGTATCAGT 
AGTTTAAAGA AGCTTTGGGA GAACAAAGAA TCTTGTGACA GTCAGCCTCA TAGTCCAAAA 
CTCAATGTCA GAGGAAGTGG TGGTAAACAA GAAATGATAG ACCAGACAGA AGACTCACCG 
GAGGATCATA GTGGAGCTTC GACACGTAGT CAGAGTTCCG GTAGCAAAAG TGACGCTAGA 
CTATGGCCAC CGCCCGAACC TGAAAAACCG GCAGTTCCAG CAAAGCCATT AAAACCACTA 
TGTTCATCAA CGAAGCACTT TGGTTCTTCG ATTTACGCAA CGCCAAACTG TACGAAATCT 
CAGCACGCTG AGGACGACCT AAACAAACAG AATACAGATT CAAAGACTGC GAAACAGGCA 
GTATTGGAGC TTTCGACGTT GATTGAGAGC AGCATATTAA ACTTGAAAAG TAGTCCAACA 
ATTGTAATGA CAAGTTGGCT TCAGCTCTCT GACAAGGTAG GGCTACTACA CGGAATGTGC 
ACGAATCTCG CAGACAGTGG TATCGCACCA CATGCGCGGT TTCAATTTCG CGAACTTCTT 
GCAAGATTAG AACTTCAGGC ACGGCAGCTT CGTGCAGCCG GTATTCGGAA CGTTGCAGAG 
AATACAAGGC TTCTCACTGA TCTTCAAAAC ACGATAAAGG ATGTTGTTAA TGCTGTGCAA 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100878636 ( XM_012286436.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012141826.1 Megachile rotundata tyrosine-protein kinase Abl (LOC100878636), transcript variant X4, mRNA.

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CloneID	OMe105645
Clone ID Related Accession (Same CDS sequence)	XM_012286436.1
Accession Version	XM_012286436.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	tyrosine-protein kinase Abl isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797180.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGCTC AACAGACTAA GGAAAGAATT GTTCCTGCCG GCTCTACTGC GCGACAGACT 
CGCAAACAGC CAAGGAACCT CAAGGAGTCT CGTCTTGTTG GTTCCAATAT ATTTACAGAA 
CACAGCGAAG CTCTCTTACA AAGCAGACCG CTACCTCACA TTCCGGCATT ACCAGATGGC 
GATCCTCCTA GTGGCTCTGC TATCCAACCA ATTTCACAAC AAGTGAATAT TCAACAACAT 
GCTGGTGTTT CATCTACAGG GCTTTTGGAA GCTGCAAACA GGTGGACAAG TAAAGAAAAC 
TTATTAGCAC AGGAGGAAGA TGATCCACAA CTATTTGTGG CTCTATATGA TTTTCAAGCA 
GGTGGAGAAA ACCAACTTAG TCTTAAAAAG GGAGAGCAAG TTCGTATTCT AAGTTATAAT 
AAAAGTGGAG AATGGTGTGA AGCTCATTCA AGTACTGGTC AAGTGGGGTG GGTTCCTTCA 
AATTATGTCA CCCCTGTAAA TTCTTTGGAA AAACATTCTT GGTATCATGG AAGGATATCC 
AGAAATGCTG CTGAATATCT CCTAAGTTCT GGTATAAATG GTAGCTTTCT GGTTCGTGAA 
TCAGAAAGCA GTCCAGGTCA ACGCAGTATT TCTTTGAGAT ATGAGGGTAG AGTCTATCAT 
TATAGGATTA ATGAGGATAG TGAAGGAAAG ATGTTTGTCA CAACAGAAAG CAAATTTAAT 
ACTCTGGCAG AATTGGTACA TCATCATTCT ATGCTTGCTG ATGGTTTAAT CACACAGCTA 
CTTTATCCTG CACCAAAGCA TAACAAACCA ACTGTTTTTC CACTTAGTCC AGAACCAGAT 
GAATGGGAGA TTAATAGGAC AGATATAGTT ATGAGGCATA AGTTAGGTGG AGGACAATAT 
GGAGATGTCT ATGAAGCAGT ATGGAAAAGA TATAACATGA CTGTGGCTGT AAAAACATTA 
AAAGAAGACA CAATGGCCCT AAAGGACTTT TTGGAGGAAG CTGCAATCAT GAAGGAGATG 
AAGCATAGGA ATTTAGTTCA GCTACTGGGT GTATGTACTC GAGAACCACC TTTCTACATC 
ATTACAGAGT TTATGAGCAA AGGAAATCTG CTAGACTATT TACGGAACGA GAGTAAACAT 
CAAATCAATG CTGTCGTTTT GATGCACATG GCCACACAGA TTGCTAGTGG AATGAGTTAT 
TTGGAGAGTA GAAATTTTAT ACACAGAGAT CTAGCAGCTC GAAACTGCCT AGTGGGTGAA 
AACCATCTGG TAAAGGTCGC AGACTTTGGC TTGGCCCGGT TGATGAGAGA TGATACGTAT 
ACGGCTCACG CTGGAGCAAA GTTTCCAATA AAATGGACTG CTCCAGAAGG ATTAGCTTAC 
AATAAATTCT CTACGAAGTC TGATGTGTGG GCATTTGGAA TTTTACTTTG GGAGATAGCA 
ACCTATGGCA TGTCCCCATA CCCTGGTGTT GATTTGACGG ATGTGTATCA CATGTTGGAG 
AAAGGCTACC GAATGGAATG TCCACCTGGA TGTCCACCGA AGGTATATGA ACTGATGCGT 
CAATGCTGGC AGTGGGCTGC TGCTGACCGG CCTACCTTCA AAGAGATTCA TCATTCTCTT 
GAAAATATGT TCCAAGAATC CAGTATTACC GAGGAAGTTG AAAAACAACT GCAAGGTGGC 
GGCGATATCC CTTTACTTTC ATATAAAAAA TCTCAAACTG GTAGTACTGG AAACATACAT 
GGGCTTGTTC TAGTCTCCGA GCCATTACCC TCTTCAGAAA CTACTAGCTC GGTAACAAAA 
CTGAGTACGT TTACGGGTGG TCTATCGAGT AAAAATAACA GCAATATCGT CCAAATGAGG 
CGTTCTACGA ATAAGAAAGG AAAACAAGCT CCGGCACCTC CAAAGAGAAC GAGCCTGCTG 
TCGTCGTGCA GTTCGTTTCG CGATTCTGCC TACCAAGAGC AAGATACCCA AAATACTGAA 
ACAAACACCA TGACTCTCGA CGATGCCACG GATCTAAATG GTATTGCGCG AGATCTCGCG 
ACGATGGCTC AAGGAACGAT TGCTGCAGGA GGCTGCGAAG TCGAGGAGGA TGGAGAGGGT 
TCTCAAGGTA CGGCGGAACC AAATTTTATC CCTCAACCTT CGACGTCCCC GGAGCCTATA 
CCTGGACTAG TAGCCTGTTC TCAGAAACAA ATCAAACCAC GACCTTATCC ATCAAAGGAA 
CCGTTACCAC AGAAGCTGGT ACAAGTGGGA GCATTAGAAG TACAGAACGT AAAAAGAGCC 
ATTAATCGTT ATGGCACACT ACCAAAAGGT GCTAGGATCG GAGCATACCT AGAATCTTTG 
CGTCAAAGTG GTATGCCATC GAATCAAGAA TCTACAACGG TGGTTTCTTC CATTGCACCT 
AGTTCAGTAG AACAACATGA GACTTCTATG GACAATTCGC AACATAGATC TCTCTCTCCT 
CGTCAAAGCA ATCTACGAAA TCAACCGCAA ATGACTCGCA GTAATTCCTC GAGCGGTGTT 
GTCAATACCT ACCAACCTCC GAATTCCCCT CGTGGCCGAG TAGTTACTGT AAGAAAGAAC 
AATCAGTCTG ATGGTGTTGG TCTGAGAACA TTTCGAGTTT CAAGTAACTC GAACTTCAGA 
ACTGCAAGTC CATCAAGATC CGTTCAACCA TCTTTAGCGG ATCTAGAGTT TCCGCCCCCA 
CCCGCAGATT TGCCTCCTCC CCCTGACGAA ACTTTCTCTG GTCAAGAACA AGCTGAACTT 
CCGCCTCCCG TTTCTTGTAC CGAGCTATCT CAGATAAGAA ATTCTCCTCT TTCTATAAGA 
AAAGCAAAGA GCACGGATTG GCGCACGAAA GAAGAAGATA ATGAACAGGA AGACAGGAAC 
GACGTTAGTA ACATAGAGCC TTCCGTCAAA GAAGCTTGTT CGAGGTTTGG AGTTAATTTA 
AGGCGCAGAG AAACTCAAGA TAATTCTTGT AGAAACTCTG ATAATAAAAG AACGGGATTT 
AAATCCAGAA TGGAGACAGT AGAGCCTGCT GCACCGCCAG AGGAAGCACC ACCTCCCCCA 
CCGCCACCTC CGCCGCCACT TTCTACGAAT ACACCCCCCG ATAGCTTCGA ACGTAAACCA 
GGCATGAAAG AAATGTTAGA ATTAAAATTG ATCAATGAAA TTAAACAGAG CGCGGAAACA 
AAACATGGTA CGACTACGAA AAAGACTGGA GCGACAAGCA ATACTCCATC CGCACCTTTA 
GATCCAGCGT CGCAGCTACT CTCGGAACTT TGTGCTAGTT TTAATATGGA GTCTGGACAG 
AGACACGCCC AAAACGAGTA CGCTGTATCA ACTTTAAAAA CCAATGACGT AAATCAAGAA 
CAACAACAGC AACAAAGTCA TAACGCTCAT AAGGATTCGT CGGTGTCCTC TCCAGTGACC 
GAGTCTCTCC TGTCCAGTGG AAATGTTGGA TTCAAGCTGA AAAGAGTTGA CAAGCGGAAT 
AATCCGCAAA AAGAAGAAAC ATCCGATGGA CAAATAATTG ATTTTAAAGC GAGACTTCGA 
AAAGTTGAGA ACGCAGAAAA AGAAAAATCC TTGGAAGAGA AAAGTACCGT CACTGAACAG 
TCTTCAGAAT CAGAAGAGCA ACAAGATGAC AAACGTAGAA GCACTGGTAG TATCAGTAGT 
TTAAAGAAGC TTTGGGAGAA CAAAGAATCT TGTGACAGTC AGCCTCATAG TCCAAAACTC 
AATGTCAGAG GAAGTGGTGG TAAACAAGAA ATGATAGACC AGACAGAAGA CTCACCGGAG 
GATCATAGTG GAGCTTCGAC ACGTAGTCAG AGTTCCGGTA GCAAAAGTGA CGCTAGACTA 
TGGCCACCGC CCGAACCTGA AAAACCGGCA GTTCCAGCAA AGCCATTAAA ACCACTATGT 
TCATCAACGA AGCACTTTGG TTCTTCGATT TACGCAACGC CAAACTGTAC GAAATCTCAG 
CACGCTGAGG ACGACCTAAA CAAACAGAAT ACAGATTCAA AGACTGCGAA ACAGGCAGTA 
TTGGAGCTTT CGACGTTGAT TGAGAGCAGC ATATTAAACT TGAAAAGTAG TCCAACAATT 
GTAATGACAA GTTGGCTTCA GCTCTCTGAC AAGGTAGGGC TACTACACGG AATGTGCACG 
AATCTCGCAG ACAGTGGTAT CGCACCACAT GCGCGGTTTC AATTTCGCGA ACTTCTTGCA 
AGATTAGAAC TTCAGGCACG GCAGCTTCGT GCAGCCGGTA TTCGGAACGT TGCAGAGAAT 
ACAAGGCTTC TCACTGATCT TCAAAACACG ATAAAGGATG TTGTTAATGC TGTGCAAAGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012141826.1
CDS	278..4600
Translation	MGAQQTKERIVPAGSTARQTRKQPRNLKESRLVGSNIFTEHSEALLQSRPLPHIPALPDGDPPSGSAIQPISQQVNIQQHAGVSSTGLLEAANRWTSKENLLAQEEDDPQLFVALYDFQAGGENQLSLKKGEQVRILSYNKSGEWCEAHSSTGQVGWVPSNYVTPVNSLEKHSWYHGRISRNAAEYLLSSGINGSFLVRESESSPGQRSISLRYEGRVYHYRINEDSEGKMFVTTESKFNTLAELVHHHSMLADGLITQLLYPAPKHNKPTVFPLSPEPDEWEINRTDIVMRHKLGGGQYGDVYEAVWKRYNMTVAVKTLKEDTMALKDFLEEAAIMKEMKHRNLVQLLGVCTREPPFYIITEFMSKGNLLDYLRNESKHQINAVVLMHMATQIASGMSYLESRNFIHRDLAARNCLVGENHLVKVADFGLARLMRDDTYTAHAGAKFPIKWTAPEGLAYNKFSTKSDVWAFGILLWEIATYGMSPYPGVDLTDVYHMLEKGYRMECPPGCPPKVYELMRQCWQWAAADRPTFKEIHHSLENMFQESSITEEVEKQLQGGGDIPLLSYKKSQTGSTGNIHGLVLVSEPLPSSETTSSVTKLSTFTGGLSSKNNSNIVQMRRSTNKKGKQAPAPPKRTSLLSSCSSFRDSAYQEQDTQNTETNTMTLDDATDLNGIARDLATMAQGTIAAGGCEVEEDGEGSQGTAEPNFIPQPSTSPEPIPGLVACSQKQIKPRPYPSKEPLPQKLVQVGALEVQNVKRAINRYGTLPKGARIGAYLESLRQSGMPSNQESTTVVSSIAPSSVEQHETSMDNSQHRSLSPRQSNLRNQPQMTRSNSSSGVVNTYQPPNSPRGRVVTVRKNNQSDGVGLRTFRVSSNSNFRTASPSRSVQPSLADLEFPPPPADLPPPPDETFSGQEQAELPPPVSCTELSQIRNSPLSIRKAKSTDWRTKEEDNEQEDRNDVSNIEPSVKEACSRFGVNLRRRETQDNSCRNSDNKRTGFKSRMETVEPAAPPEEAPPPPPPPPPPLSTNTPPDSFERKPGMKEMLELKLINEIKQSAETKHGTTTKKTGATSNTPSAPLDPASQLLSELCASFNMESGQRHAQNEYAVSTLKTNDVNQEQQQQQSHNAHKDSSVSSPVTESLLSSGNVGFKLKRVDKRNNPQKEETSDGQIIDFKARLRKVENAEKEKSLEEKSTVTEQSSESEEQQDDKRRSTGSISSLKKLWENKESCDSQPHSPKLNVRGSGGKQEMIDQTEDSPEDHSGASTRSQSSGSKSDARLWPPPEPEKPAVPAKPLKPLCSSTKHFGSSIYATPNCTKSQHAEDDLNKQNTDSKTAKQAVLELSTLIESSILNLKSSPTIVMTSWLQLSDKVGLLHGMCTNLADSGIAPHARFQFRELLARLELQARQLRAAGIRNVAENTRLLTDLQNTIKDVVNAVQR

Target ORF information:

RefSeq Version	XM_012286436.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata tyrosine-protein kinase Abl (LOC100878636), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012286436.1

ORF Insert Sequence:

ATGGGTGCTC AACAGACTAA GGAAAGAATT GTTCCTGCCG GCTCTACTGC GCGACAGACT 
CGCAAACAGC CAAGGAACCT CAAGGAGTCT CGTCTTGTTG GTTCCAATAT ATTTACAGAA 
CACAGCGAAG CTCTCTTACA AAGCAGACCG CTACCTCACA TTCCGGCATT ACCAGATGGC 
GATCCTCCTA GTGGCTCTGC TATCCAACCA ATTTCACAAC AAGTGAATAT TCAACAACAT 
GCTGGTGTTT CATCTACAGG GCTTTTGGAA GCTGCAAACA GGTGGACAAG TAAAGAAAAC 
TTATTAGCAC AGGAGGAAGA TGATCCACAA CTATTTGTGG CTCTATATGA TTTTCAAGCA 
GGTGGAGAAA ACCAACTTAG TCTTAAAAAG GGAGAGCAAG TTCGTATTCT AAGTTATAAT 
AAAAGTGGAG AATGGTGTGA AGCTCATTCA AGTACTGGTC AAGTGGGGTG GGTTCCTTCA 
AATTATGTCA CCCCTGTAAA TTCTTTGGAA AAACATTCTT GGTATCATGG AAGGATATCC 
AGAAATGCTG CTGAATATCT CCTAAGTTCT GGTATAAATG GTAGCTTTCT GGTTCGTGAA 
TCAGAAAGCA GTCCAGGTCA ACGCAGTATT TCTTTGAGAT ATGAGGGTAG AGTCTATCAT 
TATAGGATTA ATGAGGATAG TGAAGGAAAG ATGTTTGTCA CAACAGAAAG CAAATTTAAT 
ACTCTGGCAG AATTGGTACA TCATCATTCT ATGCTTGCTG ATGGTTTAAT CACACAGCTA 
CTTTATCCTG CACCAAAGCA TAACAAACCA ACTGTTTTTC CACTTAGTCC AGAACCAGAT 
GAATGGGAGA TTAATAGGAC AGATATAGTT ATGAGGCATA AGTTAGGTGG AGGACAATAT 
GGAGATGTCT ATGAAGCAGT ATGGAAAAGA TATAACATGA CTGTGGCTGT AAAAACATTA 
AAAGAAGACA CAATGGCCCT AAAGGACTTT TTGGAGGAAG CTGCAATCAT GAAGGAGATG 
AAGCATAGGA ATTTAGTTCA GCTACTGGGT GTATGTACTC GAGAACCACC TTTCTACATC 
ATTACAGAGT TTATGAGCAA AGGAAATCTG CTAGACTATT TACGGAACGA GAGTAAACAT 
CAAATCAATG CTGTCGTTTT GATGCACATG GCCACACAGA TTGCTAGTGG AATGAGTTAT 
TTGGAGAGTA GAAATTTTAT ACACAGAGAT CTAGCAGCTC GAAACTGCCT AGTGGGTGAA 
AACCATCTGG TAAAGGTCGC AGACTTTGGC TTGGCCCGGT TGATGAGAGA TGATACGTAT 
ACGGCTCACG CTGGAGCAAA GTTTCCAATA AAATGGACTG CTCCAGAAGG ATTAGCTTAC 
AATAAATTCT CTACGAAGTC TGATGTGTGG GCATTTGGAA TTTTACTTTG GGAGATAGCA 
ACCTATGGCA TGTCCCCATA CCCTGGTGTT GATTTGACGG ATGTGTATCA CATGTTGGAG 
AAAGGCTACC GAATGGAATG TCCACCTGGA TGTCCACCGA AGGTATATGA ACTGATGCGT 
CAATGCTGGC AGTGGGCTGC TGCTGACCGG CCTACCTTCA AAGAGATTCA TCATTCTCTT 
GAAAATATGT TCCAAGAATC CAGTATTACC GAGGAAGTTG AAAAACAACT GCAAGGTGGC 
GGCGATATCC CTTTACTTTC ATATAAAAAA TCTCAAACTG GTAGTACTGG AAACATACAT 
GGGCTTGTTC TAGTCTCCGA GCCATTACCC TCTTCAGAAA CTACTAGCTC GGTAACAAAA 
CTGAGTACGT TTACGGGTGG TCTATCGAGT AAAAATAACA GCAATATCGT CCAAATGAGG 
CGTTCTACGA ATAAGAAAGG AAAACAAGCT CCGGCACCTC CAAAGAGAAC GAGCCTGCTG 
TCGTCGTGCA GTTCGTTTCG CGATTCTGCC TACCAAGAGC AAGATACCCA AAATACTGAA 
ACAAACACCA TGACTCTCGA CGATGCCACG GATCTAAATG GTATTGCGCG AGATCTCGCG 
ACGATGGCTC AAGGAACGAT TGCTGCAGGA GGCTGCGAAG TCGAGGAGGA TGGAGAGGGT 
TCTCAAGGTA CGGCGGAACC AAATTTTATC CCTCAACCTT CGACGTCCCC GGAGCCTATA 
CCTGGACTAG TAGCCTGTTC TCAGAAACAA ATCAAACCAC GACCTTATCC ATCAAAGGAA 
CCGTTACCAC AGAAGCTGGT ACAAGTGGGA GCATTAGAAG TACAGAACGT AAAAAGAGCC 
ATTAATCGTT ATGGCACACT ACCAAAAGGT GCTAGGATCG GAGCATACCT AGAATCTTTG 
CGTCAAAGTG GTATGCCATC GAATCAAGAA TCTACAACGG TGGTTTCTTC CATTGCACCT 
AGTTCAGTAG AACAACATGA GACTTCTATG GACAATTCGC AACATAGATC TCTCTCTCCT 
CGTCAAAGCA ATCTACGAAA TCAACCGCAA ATGACTCGCA GTAATTCCTC GAGCGGTGTT 
GTCAATACCT ACCAACCTCC GAATTCCCCT CGTGGCCGAG TAGTTACTGT AAGAAAGAAC 
AATCAGTCTG ATGGTGTTGG TCTGAGAACA TTTCGAGTTT CAAGTAACTC GAACTTCAGA 
ACTGCAAGTC CATCAAGATC CGTTCAACCA TCTTTAGCGG ATCTAGAGTT TCCGCCCCCA 
CCCGCAGATT TGCCTCCTCC CCCTGACGAA ACTTTCTCTG GTCAAGAACA AGCTGAACTT 
CCGCCTCCCG TTTCTTGTAC CGAGCTATCT CAGATAAGAA ATTCTCCTCT TTCTATAAGA 
AAAGCAAAGA GCACGGATTG GCGCACGAAA GAAGAAGATA ATGAACAGGA AGACAGGAAC 
GACGTTAGTA ACATAGAGCC TTCCGTCAAA GAAGCTTGTT CGAGGTTTGG AGTTAATTTA 
AGGCGCAGAG AAACTCAAGA TAATTCTTGT AGAAACTCTG ATAATAAAAG AACGGGATTT 
AAATCCAGAA TGGAGACAGT AGAGCCTGCT GCACCGCCAG AGGAAGCACC ACCTCCCCCA 
CCGCCACCTC CGCCGCCACT TTCTACGAAT ACACCCCCCG ATAGCTTCGA ACGTAAACCA 
GGCATGAAAG AAATGTTAGA ATTAAAATTG ATCAATGAAA TTAAACAGAG CGCGGAAACA 
AAACATGGTA CGACTACGAA AAAGACTGGA GCGACAAGCA ATACTCCATC CGCACCTTTA 
GATCCAGCGT CGCAGCTACT CTCGGAACTT TGTGCTAGTT TTAATATGGA GTCTGGACAG 
AGACACGCCC AAAACGAGTA CGCTGTATCA ACTTTAAAAA CCAATGACGT AAATCAAGAA 
CAACAACAGC AACAAAGTCA TAACGCTCAT AAGGATTCGT CGGTGTCCTC TCCAGTGACC 
GAGTCTCTCC TGTCCAGTGG AAATGTTGGA TTCAAGCTGA AAAGAGTTGA CAAGCGGAAT 
AATCCGCAAA AAGAAGAAAC ATCCGATGGA CAAATAATTG ATTTTAAAGC GAGACTTCGA 
AAAGTTGAGA ACGCAGAAAA AGAAAAATCC TTGGAAGAGA AAAGTACCGT CACTGAACAG 
TCTTCAGAAT CAGAAGAGCA ACAAGATGAC AAACGTAGAA GCACTGGTAG TATCAGTAGT 
TTAAAGAAGC TTTGGGAGAA CAAAGAATCT TGTGACAGTC AGCCTCATAG TCCAAAACTC 
AATGTCAGAG GAAGTGGTGG TAAACAAGAA ATGATAGACC AGACAGAAGA CTCACCGGAG 
GATCATAGTG GAGCTTCGAC ACGTAGTCAG AGTTCCGGTA GCAAAAGTGA CGCTAGACTA 
TGGCCACCGC CCGAACCTGA AAAACCGGCA GTTCCAGCAA AGCCATTAAA ACCACTATGT 
TCATCAACGA AGCACTTTGG TTCTTCGATT TACGCAACGC CAAACTGTAC GAAATCTCAG 
CACGCTGAGG ACGACCTAAA CAAACAGAAT ACAGATTCAA AGACTGCGAA ACAGGCAGTA 
TTGGAGCTTT CGACGTTGAT TGAGAGCAGC ATATTAAACT TGAAAAGTAG TCCAACAATT 
GTAATGACAA GTTGGCTTCA GCTCTCTGAC AAGGTAGGGC TACTACACGG AATGTGCACG 
AATCTCGCAG ACAGTGGTAT CGCACCACAT GCGCGGTTTC AATTTCGCGA ACTTCTTGCA 
AGATTAGAAC TTCAGGCACG GCAGCTTCGT GCAGCCGGTA TTCGGAACGT TGCAGAGAAT 
ACAAGGCTTC TCACTGATCT TCAAAACACG ATAAAGGATG TTGTTAATGC TGTGCAAAGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100878636 ( XM_012286437.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012141827.1 Megachile rotundata tyrosine-protein kinase Abl (LOC100878636), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMe105646
Clone ID Related Accession (Same CDS sequence)	XM_012286437.1
Accession Version	XM_012286437.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	tyrosine-protein kinase Abl isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797180.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGCTC AACAGACTAA GGAAAGAATT GTTCCTGCCG GCTCTACTGC GCGACAGACT 
CGCAAACAGC CAAGGAACCT CAAGGAGTCT CGTCTTGTTG GTTCCAATAT ATTTACAGAA 
CACAGCGAAG CTCTCTTACA AAGCAGACCG CTACCTCACA TTCCGGCATT ACCAGATGGC 
GATCCTCCTA GTGGCTCTGC TATCCAACCA ATTTCACAAC AAGTGAATAT TCAACAACAT 
GCTGGTGTTT CATCTACAGG GCTTTTGGAA GCTGCAAACA GGTGGACAAG TAAAGAAAAC 
TTATTAGCAC AGGAGGAAGA TGATCCACAA CTATTTGTGG CTCTATATGA TTTTCAAGCA 
GGTGGAGAAA ACCAACTTAG TCTTAAAAAG GGAGAGCAAG TTCGTATTCT AAGTTATAAT 
AAAAGTGGAG AATGGTGTGA AGCTCATTCA AGTACTGGTC AAGTGGGGTG GGTTCCTTCA 
AATTATGTCA CCCCTGTAAA TTCTTTGGAA AAACATTCTT GGTATCATGG AAGGATATCC 
AGAAATGCTG CTGAATATCT CCTAAGTTCT GGTATAAATG GTAGCTTTCT GGTTCGTGAA 
TCAGAAAGCA GTCCAGGTCA ACGCAGTATT TCTTTGAGAT ATGAGGGTAG AGTCTATCAT 
TATAGGATTA ATGAGGATAG TGAAGGAAAG ATGTTTGTCA CAACAGAAAG CAAATTTAAT 
ACTCTGGCAG AATTGGTACA TCATCATTCT ATGCTTGCTG ATGGTTTAAT CACACAGCTA 
CTTTATCCTG CACCAAAGCA TAACAAACCA ACTGTTTTTC CACTTAGTCC AGAACCAGAT 
GAATGGGAGA TTAATAGGAC AGATATAGTT ATGAGGCATA AGTTAGGTGG AGGACAATAT 
GGAGATGTCT ATGAAGCAGT ATGGAAAAGA TATAACATGA CTGTGGCTGT AAAAACATTA 
AAAGAAGACA CAATGGCCCT AAAGGACTTT TTGGAGGAAG CTGCAATCAT GAAGGAGATG 
AAGCATAGGA ATTTAGTTCA GCTACTGGGT GTATGTACTC GAGAACCACC TTTCTACATC 
ATTACAGAGT TTATGAGCAA AGGAAATCTG CTAGACTATT TACGGAACGA GAGTAAACAT 
CAAATCAATG CTGTCGTTTT GATGCACATG GCCACACAGA TTGCTAGTGG AATGAGTTAT 
TTGGAGAGTA GAAATTTTAT ACACAGAGAT CTAGCAGCTC GAAACTGCCT AGTGGGTGAA 
AACCATCTGG TAAAGGTCGC AGACTTTGGC TTGGCCCGGT TGATGAGAGA TGATACGTAT 
ACGGCTCACG CTGGAGCAAA GTTTCCAATA AAATGGACTG CTCCAGAAGG ATTAGCTTAC 
AATAAATTCT CTACGAAGTC TGATGTGTGG GCATTTGGAA TTTTACTTTG GGAGATAGCA 
ACCTATGGCA TGTCCCCATA CCCTGGTGTT GATTTGACGG ATGTGTATCA CATGTTGGAG 
AAAGGCTACC GAATGGAATG TCCACCTGGA TGTCCACCGA AGGTATATGA ACTGATGCGT 
CAATGCTGGC AGTGGGCTGC TGCTGACCGG CCTACCTTCA AAGAGATTCA TCATTCTCTT 
GAAAATATGT TCCAAGAATC CAGTATTACC GAGGAAGTTG AAAAACAACT GCAAGGTGGC 
GGCGATATCC CTTTACTTTC ATATAAAAAA TCTCAAACTG GTAGTACTGG AAACATACAT 
GGGCTTGTTC TAGTCTCCGA GCCATTACCC TCTTCAGAAA CTACTAGCTC GGTAACAAAA 
CTGAGTACGT TTACGGGTGG TCTATCGAGT AAAAATAACA GCAATATCGT CCAAATGAGG 
CGTTCTACGA ATAAGAAAGG AAAACAAGCT CCGGCACCTC CAAAGAGAAC GAGCCTGCTG 
TCGTCGTGCA GTTCGTTTCG CGATTCTGCC TACCAAGAGC AAGATACCCA AAATACTGAA 
ACAAACACCA TGACTCTCGA CGATGCCACG GATCTAAATG GTATTGATAA GATTCTCGAA 
GGAGGCTGCG AAGTCGAGGA GGATGGAGAG GGTTCTCAAG GTACGGCGGA ACCAAATTTT 
ATCCCTCAAC CTTCGACGTC CCCGGAGCCT ATACCTGGAC TAGTAGCCTG TTCTCAGAAA 
CAAATCAAAC CACGACCTTA TCCATCAAAG GAACCGTTAC CACAGAAGCT GGTACAAGTG 
GGAGCATTAG AAGTACAGAA CGTAAAAAGA GCCATTAATC GTTATGGCAC ACTACCAAAA 
GGTGCTAGGA TCGGAGCATA CCTAGAATCT TTGCGTCAAA GTGGTATGCC ATCGAATCAA 
GAATCTACAA CGGTGGTTTC TTCCATTGCA CCTAGTTCAG TAGAACAACA TGAGACTTCT 
ATGGACAATT CGCAACATAG ATCTCTCTCT CCTCGTCAAA GCAATCTACG AAATCAACCG 
CAAATGACTC GCAGTAATTC CTCGAGCGGT GTTGTCAATA CCTACCAACC TCCGAATTCC 
CCTCGTGGCC GAGTAGTTAC TGTAAGAAAG AACAATCAGT CTGATGGTGT TGGTCTGAGA 
ACATTTCGAG TTTCAAGTAA CTCGAACTTC AGAACTGCAA GTCCATCAAG ATCCGTTCAA 
CCATCTTTAG CGGATCTAGA GTTTCCGCCC CCACCCGCAG ATTTGCCTCC TCCCCCTGAC 
GAAACTTTCT CTGGTCAAGA ACAAGCTGAA CTTCCGCCTC CCGTTTCTTG TACCGAGCTA 
TCTCAGATAA GAAATTCTCC TCTTTCTATA AGAAAAGCAA AGAGCACGGA TTGGCGCACG 
AAAGAAGAAG ATAATGAACA GGAAGACAGG AACGACGTTA GTAACATAGA GCCTTCCGTC 
AAAGAAGCTT GTTCGAGGTT TGGAGTTAAT TTAAGGCGCA GAGAAACTCA AGATAATTCT 
TGTAGAAACT CTGATAATAA AAGAACGGGA TTTAAATCCA GAATGGAGAC AGTAGAGCCT 
GCTGCACCGC CAGAGGAAGC ACCACCTCCC CCACCGCCAC CTCCGCCGCC ACTTTCTACG 
AATACACCCC CCGATAGCTT CGAACGTAAA CCAGGCATGA AAGAAATGTT AGAATTAAAA 
TTGATCAATG AAATTAAACA GAGCGCGGAA ACAAAACATG GTACGACTAC GAAAAAGACT 
GGAGCGACAA GCAATACTCC ATCCGCACCT TTAGATCCAG CGTCGCAGCT ACTCTCGGAA 
CTTTGTGCTA GTTTTAATAT GGAGTCTGGA CAGAGACACG CCCAAAACGA GTACGCTGTA 
TCAACTTTAA AAACCAATGA CGTAAATCAA GAACAACAAC AGCAACAAAG TCATAACGCT 
CATAAGGATT CGTCGGTGTC CTCTCCAGTG ACCGAGTCTC TCCTGTCCAG TGGAAATGTT 
GGATTCAAGC TGAAAAGAGT TGACAAGCGG AATAATCCGC AAAAAGAAGA AACATCCGAT 
GGACAAATAA TTGATTTTAA AGCGAGACTT CGAAAAGTTG AGAACGCAGA AAAAGAAAAA 
TCCTTGGAAG AGAAAAGTAC CGTCACTGAA CAGTCTTCAG AATCAGAAGA GCAACAAGAT 
GACAAACGTA GAAGCACTGG TAGTATCAGT AGTTTAAAGA AGCTTTGGGA GAACAAAGAA 
TCTTGTGACA GTCAGCCTCA TAGTCCAAAA CTCAATGTCA GAGGAAGTGG TGGTAAACAA 
GAAATGATAG ACCAGACAGA AGACTCACCG GAGGATCATA GTGGAGCTTC GACACGTAGT 
CAGAGTTCCG GTAGCAAAAG TGACGCTAGA CTATGGCCAC CGCCCGAACC TGAAAAACCG 
GCAGTTCCAG CAAAGCCATT AAAACCACTA TGTTCATCAA CGAAGCACTT TGGTTCTTCG 
ATTTACGCAA CGCCAAACTG TACGAAATCT CAGCACGCTG AGGACGACCT AAACAAACAG 
AATACAGATT CAAAGACTGC GAAACAGGCA GTATTGGAGC TTTCGACGTT GATTGAGAGC 
AGCATATTAA ACTTGAAAAG TAGTCCAACA ATTGTAATGA CAAGTTGGCT TCAGCTCTCT 
GACAAGGTAG GGCTACTACA CGGAATGTGC ACGAATCTCG CAGACAGTGG TATCGCACCA 
CATGCGCGGT TTCAATTTCG CGAACTTCTT GCAAGATTAG AACTTCAGGC ACGGCAGCTT 
CGTGCAGCCG GTATTCGGAA CGTTGCAGAG AATACAAGGC TTCTCACTGA TCTTCAAAAC 
ACGATAAAGG ATGTTGTTAA TGCTGTGCAA AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012141827.1
CDS	278..4573
Translation	MGAQQTKERIVPAGSTARQTRKQPRNLKESRLVGSNIFTEHSEALLQSRPLPHIPALPDGDPPSGSAIQPISQQVNIQQHAGVSSTGLLEAANRWTSKENLLAQEEDDPQLFVALYDFQAGGENQLSLKKGEQVRILSYNKSGEWCEAHSSTGQVGWVPSNYVTPVNSLEKHSWYHGRISRNAAEYLLSSGINGSFLVRESESSPGQRSISLRYEGRVYHYRINEDSEGKMFVTTESKFNTLAELVHHHSMLADGLITQLLYPAPKHNKPTVFPLSPEPDEWEINRTDIVMRHKLGGGQYGDVYEAVWKRYNMTVAVKTLKEDTMALKDFLEEAAIMKEMKHRNLVQLLGVCTREPPFYIITEFMSKGNLLDYLRNESKHQINAVVLMHMATQIASGMSYLESRNFIHRDLAARNCLVGENHLVKVADFGLARLMRDDTYTAHAGAKFPIKWTAPEGLAYNKFSTKSDVWAFGILLWEIATYGMSPYPGVDLTDVYHMLEKGYRMECPPGCPPKVYELMRQCWQWAAADRPTFKEIHHSLENMFQESSITEEVEKQLQGGGDIPLLSYKKSQTGSTGNIHGLVLVSEPLPSSETTSSVTKLSTFTGGLSSKNNSNIVQMRRSTNKKGKQAPAPPKRTSLLSSCSSFRDSAYQEQDTQNTETNTMTLDDATDLNGIDKILEGGCEVEEDGEGSQGTAEPNFIPQPSTSPEPIPGLVACSQKQIKPRPYPSKEPLPQKLVQVGALEVQNVKRAINRYGTLPKGARIGAYLESLRQSGMPSNQESTTVVSSIAPSSVEQHETSMDNSQHRSLSPRQSNLRNQPQMTRSNSSSGVVNTYQPPNSPRGRVVTVRKNNQSDGVGLRTFRVSSNSNFRTASPSRSVQPSLADLEFPPPPADLPPPPDETFSGQEQAELPPPVSCTELSQIRNSPLSIRKAKSTDWRTKEEDNEQEDRNDVSNIEPSVKEACSRFGVNLRRRETQDNSCRNSDNKRTGFKSRMETVEPAAPPEEAPPPPPPPPPPLSTNTPPDSFERKPGMKEMLELKLINEIKQSAETKHGTTTKKTGATSNTPSAPLDPASQLLSELCASFNMESGQRHAQNEYAVSTLKTNDVNQEQQQQQSHNAHKDSSVSSPVTESLLSSGNVGFKLKRVDKRNNPQKEETSDGQIIDFKARLRKVENAEKEKSLEEKSTVTEQSSESEEQQDDKRRSTGSISSLKKLWENKESCDSQPHSPKLNVRGSGGKQEMIDQTEDSPEDHSGASTRSQSSGSKSDARLWPPPEPEKPAVPAKPLKPLCSSTKHFGSSIYATPNCTKSQHAEDDLNKQNTDSKTAKQAVLELSTLIESSILNLKSSPTIVMTSWLQLSDKVGLLHGMCTNLADSGIAPHARFQFRELLARLELQARQLRAAGIRNVAENTRLLTDLQNTIKDVVNAVQR

Target ORF information:

RefSeq Version	XM_012286437.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata tyrosine-protein kinase Abl (LOC100878636), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012286437.1

ORF Insert Sequence:

ATGGGTGCTC AACAGACTAA GGAAAGAATT GTTCCTGCCG GCTCTACTGC GCGACAGACT 
CGCAAACAGC CAAGGAACCT CAAGGAGTCT CGTCTTGTTG GTTCCAATAT ATTTACAGAA 
CACAGCGAAG CTCTCTTACA AAGCAGACCG CTACCTCACA TTCCGGCATT ACCAGATGGC 
GATCCTCCTA GTGGCTCTGC TATCCAACCA ATTTCACAAC AAGTGAATAT TCAACAACAT 
GCTGGTGTTT CATCTACAGG GCTTTTGGAA GCTGCAAACA GGTGGACAAG TAAAGAAAAC 
TTATTAGCAC AGGAGGAAGA TGATCCACAA CTATTTGTGG CTCTATATGA TTTTCAAGCA 
GGTGGAGAAA ACCAACTTAG TCTTAAAAAG GGAGAGCAAG TTCGTATTCT AAGTTATAAT 
AAAAGTGGAG AATGGTGTGA AGCTCATTCA AGTACTGGTC AAGTGGGGTG GGTTCCTTCA 
AATTATGTCA CCCCTGTAAA TTCTTTGGAA AAACATTCTT GGTATCATGG AAGGATATCC 
AGAAATGCTG CTGAATATCT CCTAAGTTCT GGTATAAATG GTAGCTTTCT GGTTCGTGAA 
TCAGAAAGCA GTCCAGGTCA ACGCAGTATT TCTTTGAGAT ATGAGGGTAG AGTCTATCAT 
TATAGGATTA ATGAGGATAG TGAAGGAAAG ATGTTTGTCA CAACAGAAAG CAAATTTAAT 
ACTCTGGCAG AATTGGTACA TCATCATTCT ATGCTTGCTG ATGGTTTAAT CACACAGCTA 
CTTTATCCTG CACCAAAGCA TAACAAACCA ACTGTTTTTC CACTTAGTCC AGAACCAGAT 
GAATGGGAGA TTAATAGGAC AGATATAGTT ATGAGGCATA AGTTAGGTGG AGGACAATAT 
GGAGATGTCT ATGAAGCAGT ATGGAAAAGA TATAACATGA CTGTGGCTGT AAAAACATTA 
AAAGAAGACA CAATGGCCCT AAAGGACTTT TTGGAGGAAG CTGCAATCAT GAAGGAGATG 
AAGCATAGGA ATTTAGTTCA GCTACTGGGT GTATGTACTC GAGAACCACC TTTCTACATC 
ATTACAGAGT TTATGAGCAA AGGAAATCTG CTAGACTATT TACGGAACGA GAGTAAACAT 
CAAATCAATG CTGTCGTTTT GATGCACATG GCCACACAGA TTGCTAGTGG AATGAGTTAT 
TTGGAGAGTA GAAATTTTAT ACACAGAGAT CTAGCAGCTC GAAACTGCCT AGTGGGTGAA 
AACCATCTGG TAAAGGTCGC AGACTTTGGC TTGGCCCGGT TGATGAGAGA TGATACGTAT 
ACGGCTCACG CTGGAGCAAA GTTTCCAATA AAATGGACTG CTCCAGAAGG ATTAGCTTAC 
AATAAATTCT CTACGAAGTC TGATGTGTGG GCATTTGGAA TTTTACTTTG GGAGATAGCA 
ACCTATGGCA TGTCCCCATA CCCTGGTGTT GATTTGACGG ATGTGTATCA CATGTTGGAG 
AAAGGCTACC GAATGGAATG TCCACCTGGA TGTCCACCGA AGGTATATGA ACTGATGCGT 
CAATGCTGGC AGTGGGCTGC TGCTGACCGG CCTACCTTCA AAGAGATTCA TCATTCTCTT 
GAAAATATGT TCCAAGAATC CAGTATTACC GAGGAAGTTG AAAAACAACT GCAAGGTGGC 
GGCGATATCC CTTTACTTTC ATATAAAAAA TCTCAAACTG GTAGTACTGG AAACATACAT 
GGGCTTGTTC TAGTCTCCGA GCCATTACCC TCTTCAGAAA CTACTAGCTC GGTAACAAAA 
CTGAGTACGT TTACGGGTGG TCTATCGAGT AAAAATAACA GCAATATCGT CCAAATGAGG 
CGTTCTACGA ATAAGAAAGG AAAACAAGCT CCGGCACCTC CAAAGAGAAC GAGCCTGCTG 
TCGTCGTGCA GTTCGTTTCG CGATTCTGCC TACCAAGAGC AAGATACCCA AAATACTGAA 
ACAAACACCA TGACTCTCGA CGATGCCACG GATCTAAATG GTATTGATAA GATTCTCGAA 
GGAGGCTGCG AAGTCGAGGA GGATGGAGAG GGTTCTCAAG GTACGGCGGA ACCAAATTTT 
ATCCCTCAAC CTTCGACGTC CCCGGAGCCT ATACCTGGAC TAGTAGCCTG TTCTCAGAAA 
CAAATCAAAC CACGACCTTA TCCATCAAAG GAACCGTTAC CACAGAAGCT GGTACAAGTG 
GGAGCATTAG AAGTACAGAA CGTAAAAAGA GCCATTAATC GTTATGGCAC ACTACCAAAA 
GGTGCTAGGA TCGGAGCATA CCTAGAATCT TTGCGTCAAA GTGGTATGCC ATCGAATCAA 
GAATCTACAA CGGTGGTTTC TTCCATTGCA CCTAGTTCAG TAGAACAACA TGAGACTTCT 
ATGGACAATT CGCAACATAG ATCTCTCTCT CCTCGTCAAA GCAATCTACG AAATCAACCG 
CAAATGACTC GCAGTAATTC CTCGAGCGGT GTTGTCAATA CCTACCAACC TCCGAATTCC 
CCTCGTGGCC GAGTAGTTAC TGTAAGAAAG AACAATCAGT CTGATGGTGT TGGTCTGAGA 
ACATTTCGAG TTTCAAGTAA CTCGAACTTC AGAACTGCAA GTCCATCAAG ATCCGTTCAA 
CCATCTTTAG CGGATCTAGA GTTTCCGCCC CCACCCGCAG ATTTGCCTCC TCCCCCTGAC 
GAAACTTTCT CTGGTCAAGA ACAAGCTGAA CTTCCGCCTC CCGTTTCTTG TACCGAGCTA 
TCTCAGATAA GAAATTCTCC TCTTTCTATA AGAAAAGCAA AGAGCACGGA TTGGCGCACG 
AAAGAAGAAG ATAATGAACA GGAAGACAGG AACGACGTTA GTAACATAGA GCCTTCCGTC 
AAAGAAGCTT GTTCGAGGTT TGGAGTTAAT TTAAGGCGCA GAGAAACTCA AGATAATTCT 
TGTAGAAACT CTGATAATAA AAGAACGGGA TTTAAATCCA GAATGGAGAC AGTAGAGCCT 
GCTGCACCGC CAGAGGAAGC ACCACCTCCC CCACCGCCAC CTCCGCCGCC ACTTTCTACG 
AATACACCCC CCGATAGCTT CGAACGTAAA CCAGGCATGA AAGAAATGTT AGAATTAAAA 
TTGATCAATG AAATTAAACA GAGCGCGGAA ACAAAACATG GTACGACTAC GAAAAAGACT 
GGAGCGACAA GCAATACTCC ATCCGCACCT TTAGATCCAG CGTCGCAGCT ACTCTCGGAA 
CTTTGTGCTA GTTTTAATAT GGAGTCTGGA CAGAGACACG CCCAAAACGA GTACGCTGTA 
TCAACTTTAA AAACCAATGA CGTAAATCAA GAACAACAAC AGCAACAAAG TCATAACGCT 
CATAAGGATT CGTCGGTGTC CTCTCCAGTG ACCGAGTCTC TCCTGTCCAG TGGAAATGTT 
GGATTCAAGC TGAAAAGAGT TGACAAGCGG AATAATCCGC AAAAAGAAGA AACATCCGAT 
GGACAAATAA TTGATTTTAA AGCGAGACTT CGAAAAGTTG AGAACGCAGA AAAAGAAAAA 
TCCTTGGAAG AGAAAAGTAC CGTCACTGAA CAGTCTTCAG AATCAGAAGA GCAACAAGAT 
GACAAACGTA GAAGCACTGG TAGTATCAGT AGTTTAAAGA AGCTTTGGGA GAACAAAGAA 
TCTTGTGACA GTCAGCCTCA TAGTCCAAAA CTCAATGTCA GAGGAAGTGG TGGTAAACAA 
GAAATGATAG ACCAGACAGA AGACTCACCG GAGGATCATA GTGGAGCTTC GACACGTAGT 
CAGAGTTCCG GTAGCAAAAG TGACGCTAGA CTATGGCCAC CGCCCGAACC TGAAAAACCG 
GCAGTTCCAG CAAAGCCATT AAAACCACTA TGTTCATCAA CGAAGCACTT TGGTTCTTCG 
ATTTACGCAA CGCCAAACTG TACGAAATCT CAGCACGCTG AGGACGACCT AAACAAACAG 
AATACAGATT CAAAGACTGC GAAACAGGCA GTATTGGAGC TTTCGACGTT GATTGAGAGC 
AGCATATTAA ACTTGAAAAG TAGTCCAACA ATTGTAATGA CAAGTTGGCT TCAGCTCTCT 
GACAAGGTAG GGCTACTACA CGGAATGTGC ACGAATCTCG CAGACAGTGG TATCGCACCA 
CATGCGCGGT TTCAATTTCG CGAACTTCTT GCAAGATTAG AACTTCAGGC ACGGCAGCTT 
CGTGCAGCCG GTATTCGGAA CGTTGCAGAG AATACAAGGC TTCTCACTGA TCTTCAAAAC 
ACGATAAAGG ATGTTGTTAA TGCTGTGCAA AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100878636 ( XM_012286439.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012141829.1 Megachile rotundata tyrosine-protein kinase Abl (LOC100878636), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMe105647
Clone ID Related Accession (Same CDS sequence)	XM_012286439.1
Accession Version	XM_012286439.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	tyrosine-protein kinase Abl isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797180.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGCTC AACAGACTAA GGAAAGAATT GTTCCTGCCG GCTCTACTGC GCGACAGACT 
CGCAAACAGC CAAGGAACCT CAAGGAGTCT CGTCTTGTTG GTTCCAATAT ATTTACAGAA 
CACAGCGAAG CTCTCTTACA AAGCAGACCG CTACCTCACA TTCCGGCATT ACCAGATGGC 
GATCCTCCTA GTGGCTCTGC TATCCAACCA ATTTCACAAC AAGTGAATAT TCAACAACAT 
GCTGGTGTTT CATCTACAGG GCTTTTGGAA GCTGCAAACA GGTGGACAAG TAAAGAAAAC 
TTATTAGCAC AGGAGGAAGA TGATCCACAA CTATTTGTGG CTCTATATGA TTTTCAAGCA 
GGTGGAGAAA ACCAACTTAG TCTTAAAAAG GGAGAGCAAG TTCGTATTCT AAGTTATAAT 
AAAAGTGGAG AATGGTGTGA AGCTCATTCA AGTACTGGTC AAGTGGGGTG GGTTCCTTCA 
AATTATGTCA CCCCTGTAAA TTCTTTGGAA AAACATTCTT GGTATCATGG AAGGATATCC 
AGAAATGCTG CTGAATATCT CCTAAGTTCT GGTATAAATG GTAGCTTTCT GGTTCGTGAA 
TCAGAAAGCA GTCCAGGTCA ACGCAGTATT TCTTTGAGAT ATGAGGGTAG AGTCTATCAT 
TATAGGATTA ATGAGGATAG TGAAGGAAAG ATGTTTGTCA CAACAGAAAG CAAATTTAAT 
ACTCTGGCAG AATTGGTACA TCATCATTCT ATGCTTGCTG ATGGTTTAAT CACACAGCTA 
CTTTATCCTG CACCAAAGCA TAACAAACCA ACTGTTTTTC CACTTAGTCC AGAACCAGAT 
GAATGGGAGA TTAATAGGAC AGATATAGTT ATGAGGCATA AGTTAGGTGG AGGACAATAT 
GGAGATGTCT ATGAAGCAGT ATGGAAAAGA TATAACATGA CTGTGGCTGT AAAAACATTA 
AAAGAAGACA CAATGGCCCT AAAGGACTTT TTGGAGGAAG CTGCAATCAT GAAGGAGATG 
AAGCATAGGA ATTTAGTTCA GCTACTGGGT GTATGTACTC GAGAACCACC TTTCTACATC 
ATTACAGAGT TTATGAGCAA AGGAAATCTG CTAGACTATT TACGGAACGA GAGTAAACAT 
CAAATCAATG CTGTCGTTTT GATGCACATG GCCACACAGA TTGCTAGTGG AATGAGTTAT 
TTGGAGAGTA GAAATTTTAT ACACAGAGAT CTAGCAGCTC GAAACTGCCT AGTGGGTGAA 
AACCATCTGG TAAAGGTCGC AGACTTTGGC TTGGCCCGGT TGATGAGAGA TGATACGTAT 
ACGGCTCACG CTGGAGCAAA GTTTCCAATA AAATGGACTG CTCCAGAAGG ATTAGCTTAC 
AATAAATTCT CTACGAAGTC TGATGTGTGG GCATTTGGAA TTTTACTTTG GGAGATAGCA 
ACCTATGGCA TGTCCCCATA CCCTGGTGTT GATTTGACGG ATGTGTATCA CATGTTGGAG 
AAAGGCTACC GAATGGAATG TCCACCTGGA TGTCCACCGA AGGTATATGA ACTGATGCGT 
CAATGCTGGC AGTGGGCTGC TGCTGACCGG CCTACCTTCA AAGAGATTCA TCATTCTCTT 
GAAAATATGT TCCAAGAATC CAGTATTACC GAGGAAGTTG AAAAACAACT GCAAGGTGGC 
GGCGATATCC CTTTACTTTC ATATAAAAAA TCTCAAACTG GTAGTACTGG AAACATACAT 
GGGCTTGTTC TAGTCTCCGA GCCATTACCC TCTTCAGAAA CTACTAGCTC GGTAACAAAA 
CTGAGTACGT TTACGGGTGG TCTATCGAGT AAAAATAACA GCAATATCGT CCAAATGAGG 
CGTTCTACGA ATAAGAAAGG AAAACAAGCT CCGGCACCTC CAAAGAGAAC GAGCCTGCTG 
TCGTCGTGCA GTTCGTTTCG CGATTCTGCC TACCAAGAGC AAGATACCCA AAATACTGAA 
ACAAACACCA TGACTCTCGA CGATGCCACG GATCTAAATG GAGGCTGCGA AGTCGAGGAG 
GATGGAGAGG GTTCTCAAGG TACGGCGGAA CCAAATTTTA TCCCTCAACC TTCGACGTCC 
CCGGAGCCTA TACCTGGACT AGTAGCCTGT TCTCAGAAAC AAATCAAACC ACGACCTTAT 
CCATCAAAGG AACCGTTACC ACAGAAGCTG GTACAAGTGG GAGCATTAGA AGTACAGAAC 
GTAAAAAGAG CCATTAATCG TTATGGCACA CTACCAAAAG GTGCTAGGAT CGGAGCATAC 
CTAGAATCTT TGCGTCAAAG TGGTATGCCA TCGAATCAAG AATCTACAAC GGTGGTTTCT 
TCCATTGCAC CTAGTTCAGT AGAACAACAT GAGACTTCTA TGGACAATTC GCAACATAGA 
TCTCTCTCTC CTCGTCAAAG CAATCTACGA AATCAACCGC AAATGACTCG CAGTAATTCC 
TCGAGCGGTG TTGTCAATAC CTACCAACCT CCGAATTCCC CTCGTGGCCG AGTAGTTACT 
GTAAGAAAGA ACAATCAGTC TGATGGTGTT GGTCTGAGAA CATTTCGAGT TTCAAGTAAC 
TCGAACTTCA GAACTGCAAG TCCATCAAGA TCCGTTCAAC CATCTTTAGC GGATCTAGAG 
TTTCCGCCCC CACCCGCAGA TTTGCCTCCT CCCCCTGACG AAACTTTCTC TGGTCAAGAA 
CAAGCTGAAC TTCCGCCTCC CGTTTCTTGT ACCGAGCTAT CTCAGATAAG AAATTCTCCT 
CTTTCTATAA GAAAAGCAAA GAGCACGGAT TGGCGCACGA AAGAAGAAGA TAATGAACAG 
GAAGACAGGA ACGACGTTAG TAACATAGAG CCTTCCGTCA AAGAAGCTTG TTCGAGGTTT 
GGAGTTAATT TAAGGCGCAG AGAAACTCAA GATAATTCTT GTAGAAACTC TGATAATAAA 
AGAACGGGAT TTAAATCCAG AATGGAGACA GTAGAGCCTG CTGCACCGCC AGAGGAAGCA 
CCACCTCCCC CACCGCCACC TCCGCCGCCA CTTTCTACGA ATACACCCCC CGATAGCTTC 
GAACGTAAAC CAGGCATGAA AGAAATGTTA GAATTAAAAT TGATCAATGA AATTAAACAG 
AGCGCGGAAA CAAAACATGG TACGACTACG AAAAAGACTG GAGCGACAAG CAATACTCCA 
TCCGCACCTT TAGATCCAGC GTCGCAGCTA CTCTCGGAAC TTTGTGCTAG TTTTAATATG 
GAGTCTGGAC AGAGACACGC CCAAAACGAG TACGCTGTAT CAACTTTAAA AACCAATGAC 
GTAAATCAAG AACAACAACA GCAACAAAGT CATAACGCTC ATAAGGATTC GTCGGTGTCC 
TCTCCAGTGA CCGAGTCTCT CCTGTCCAGT GGAAATGTTG GATTCAAGCT GAAAAGAGTT 
GACAAGCGGA ATAATCCGCA AAAAGAAGAA ACATCCGATG GACAAATAAT TGATTTTAAA 
GCGAGACTTC GAAAAGTTGA GAACGCAGAA AAAGAAAAAT CCTTGGAAGA GAAAAGTACC 
GTCACTGAAC AGTCTTCAGA ATCAGAAGAG CAACAAGATG ACAAACGTAG AAGCACTGGT 
AGTATCAGTA GTTTAAAGAA GCTTTGGGAG AACAAAGAAT CTTGTGACAG TCAGCCTCAT 
AGTCCAAAAC TCAATGTCAG AGGAAGTGGT GGTAAACAAG AAATGATAGA CCAGACAGAA 
GACTCACCGG AGGATCATAG TGGAGCTTCG ACACGTAGTC AGAGTTCCGG TAGCAAAAGT 
GACGCTAGAC TATGGCCACC GCCCGAACCT GAAAAACCGG CAGTTCCAGC AAAGCCATTA 
AAACCACTAT GTTCATCAAC GAAGCACTTT GGTTCTTCGA TTTACGCAAC GCCAAACTGT 
ACGAAATCTC AGCACGCTGA GGACGACCTA AACAAACAGA ATACAGATTC AAAGACTGCG 
AAACAGGCAG TATTGGAGCT TTCGACGTTG ATTGAGAGCA GCATATTAAA CTTGAAAAGT 
AGTCCAACAA TTGTAATGAC AAGTTGGCTT CAGCTCTCTG ACAAGGTAGG GCTACTACAC 
GGAATGTGCA CGAATCTCGC AGACAGTGGT ATCGCACCAC ATGCGCGGTT TCAATTTCGC 
GAACTTCTTG CAAGATTAGA ACTTCAGGCA CGGCAGCTTC GTGCAGCCGG TATTCGGAAC 
GTTGCAGAGA ATACAAGGCT TCTCACTGAT CTTCAAAACA CGATAAAGGA TGTTGTTAAT 
GCTGTGCAAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012141829.1
CDS	278..4552
Translation	MGAQQTKERIVPAGSTARQTRKQPRNLKESRLVGSNIFTEHSEALLQSRPLPHIPALPDGDPPSGSAIQPISQQVNIQQHAGVSSTGLLEAANRWTSKENLLAQEEDDPQLFVALYDFQAGGENQLSLKKGEQVRILSYNKSGEWCEAHSSTGQVGWVPSNYVTPVNSLEKHSWYHGRISRNAAEYLLSSGINGSFLVRESESSPGQRSISLRYEGRVYHYRINEDSEGKMFVTTESKFNTLAELVHHHSMLADGLITQLLYPAPKHNKPTVFPLSPEPDEWEINRTDIVMRHKLGGGQYGDVYEAVWKRYNMTVAVKTLKEDTMALKDFLEEAAIMKEMKHRNLVQLLGVCTREPPFYIITEFMSKGNLLDYLRNESKHQINAVVLMHMATQIASGMSYLESRNFIHRDLAARNCLVGENHLVKVADFGLARLMRDDTYTAHAGAKFPIKWTAPEGLAYNKFSTKSDVWAFGILLWEIATYGMSPYPGVDLTDVYHMLEKGYRMECPPGCPPKVYELMRQCWQWAAADRPTFKEIHHSLENMFQESSITEEVEKQLQGGGDIPLLSYKKSQTGSTGNIHGLVLVSEPLPSSETTSSVTKLSTFTGGLSSKNNSNIVQMRRSTNKKGKQAPAPPKRTSLLSSCSSFRDSAYQEQDTQNTETNTMTLDDATDLNGGCEVEEDGEGSQGTAEPNFIPQPSTSPEPIPGLVACSQKQIKPRPYPSKEPLPQKLVQVGALEVQNVKRAINRYGTLPKGARIGAYLESLRQSGMPSNQESTTVVSSIAPSSVEQHETSMDNSQHRSLSPRQSNLRNQPQMTRSNSSSGVVNTYQPPNSPRGRVVTVRKNNQSDGVGLRTFRVSSNSNFRTASPSRSVQPSLADLEFPPPPADLPPPPDETFSGQEQAELPPPVSCTELSQIRNSPLSIRKAKSTDWRTKEEDNEQEDRNDVSNIEPSVKEACSRFGVNLRRRETQDNSCRNSDNKRTGFKSRMETVEPAAPPEEAPPPPPPPPPPLSTNTPPDSFERKPGMKEMLELKLINEIKQSAETKHGTTTKKTGATSNTPSAPLDPASQLLSELCASFNMESGQRHAQNEYAVSTLKTNDVNQEQQQQQSHNAHKDSSVSSPVTESLLSSGNVGFKLKRVDKRNNPQKEETSDGQIIDFKARLRKVENAEKEKSLEEKSTVTEQSSESEEQQDDKRRSTGSISSLKKLWENKESCDSQPHSPKLNVRGSGGKQEMIDQTEDSPEDHSGASTRSQSSGSKSDARLWPPPEPEKPAVPAKPLKPLCSSTKHFGSSIYATPNCTKSQHAEDDLNKQNTDSKTAKQAVLELSTLIESSILNLKSSPTIVMTSWLQLSDKVGLLHGMCTNLADSGIAPHARFQFRELLARLELQARQLRAAGIRNVAENTRLLTDLQNTIKDVVNAVQR

Target ORF information:

RefSeq Version	XM_012286439.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata tyrosine-protein kinase Abl (LOC100878636), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012286439.1

ORF Insert Sequence:

ATGGGTGCTC AACAGACTAA GGAAAGAATT GTTCCTGCCG GCTCTACTGC GCGACAGACT 
CGCAAACAGC CAAGGAACCT CAAGGAGTCT CGTCTTGTTG GTTCCAATAT ATTTACAGAA 
CACAGCGAAG CTCTCTTACA AAGCAGACCG CTACCTCACA TTCCGGCATT ACCAGATGGC 
GATCCTCCTA GTGGCTCTGC TATCCAACCA ATTTCACAAC AAGTGAATAT TCAACAACAT 
GCTGGTGTTT CATCTACAGG GCTTTTGGAA GCTGCAAACA GGTGGACAAG TAAAGAAAAC 
TTATTAGCAC AGGAGGAAGA TGATCCACAA CTATTTGTGG CTCTATATGA TTTTCAAGCA 
GGTGGAGAAA ACCAACTTAG TCTTAAAAAG GGAGAGCAAG TTCGTATTCT AAGTTATAAT 
AAAAGTGGAG AATGGTGTGA AGCTCATTCA AGTACTGGTC AAGTGGGGTG GGTTCCTTCA 
AATTATGTCA CCCCTGTAAA TTCTTTGGAA AAACATTCTT GGTATCATGG AAGGATATCC 
AGAAATGCTG CTGAATATCT CCTAAGTTCT GGTATAAATG GTAGCTTTCT GGTTCGTGAA 
TCAGAAAGCA GTCCAGGTCA ACGCAGTATT TCTTTGAGAT ATGAGGGTAG AGTCTATCAT 
TATAGGATTA ATGAGGATAG TGAAGGAAAG ATGTTTGTCA CAACAGAAAG CAAATTTAAT 
ACTCTGGCAG AATTGGTACA TCATCATTCT ATGCTTGCTG ATGGTTTAAT CACACAGCTA 
CTTTATCCTG CACCAAAGCA TAACAAACCA ACTGTTTTTC CACTTAGTCC AGAACCAGAT 
GAATGGGAGA TTAATAGGAC AGATATAGTT ATGAGGCATA AGTTAGGTGG AGGACAATAT 
GGAGATGTCT ATGAAGCAGT ATGGAAAAGA TATAACATGA CTGTGGCTGT AAAAACATTA 
AAAGAAGACA CAATGGCCCT AAAGGACTTT TTGGAGGAAG CTGCAATCAT GAAGGAGATG 
AAGCATAGGA ATTTAGTTCA GCTACTGGGT GTATGTACTC GAGAACCACC TTTCTACATC 
ATTACAGAGT TTATGAGCAA AGGAAATCTG CTAGACTATT TACGGAACGA GAGTAAACAT 
CAAATCAATG CTGTCGTTTT GATGCACATG GCCACACAGA TTGCTAGTGG AATGAGTTAT 
TTGGAGAGTA GAAATTTTAT ACACAGAGAT CTAGCAGCTC GAAACTGCCT AGTGGGTGAA 
AACCATCTGG TAAAGGTCGC AGACTTTGGC TTGGCCCGGT TGATGAGAGA TGATACGTAT 
ACGGCTCACG CTGGAGCAAA GTTTCCAATA AAATGGACTG CTCCAGAAGG ATTAGCTTAC 
AATAAATTCT CTACGAAGTC TGATGTGTGG GCATTTGGAA TTTTACTTTG GGAGATAGCA 
ACCTATGGCA TGTCCCCATA CCCTGGTGTT GATTTGACGG ATGTGTATCA CATGTTGGAG 
AAAGGCTACC GAATGGAATG TCCACCTGGA TGTCCACCGA AGGTATATGA ACTGATGCGT 
CAATGCTGGC AGTGGGCTGC TGCTGACCGG CCTACCTTCA AAGAGATTCA TCATTCTCTT 
GAAAATATGT TCCAAGAATC CAGTATTACC GAGGAAGTTG AAAAACAACT GCAAGGTGGC 
GGCGATATCC CTTTACTTTC ATATAAAAAA TCTCAAACTG GTAGTACTGG AAACATACAT 
GGGCTTGTTC TAGTCTCCGA GCCATTACCC TCTTCAGAAA CTACTAGCTC GGTAACAAAA 
CTGAGTACGT TTACGGGTGG TCTATCGAGT AAAAATAACA GCAATATCGT CCAAATGAGG 
CGTTCTACGA ATAAGAAAGG AAAACAAGCT CCGGCACCTC CAAAGAGAAC GAGCCTGCTG 
TCGTCGTGCA GTTCGTTTCG CGATTCTGCC TACCAAGAGC AAGATACCCA AAATACTGAA 
ACAAACACCA TGACTCTCGA CGATGCCACG GATCTAAATG GAGGCTGCGA AGTCGAGGAG 
GATGGAGAGG GTTCTCAAGG TACGGCGGAA CCAAATTTTA TCCCTCAACC TTCGACGTCC 
CCGGAGCCTA TACCTGGACT AGTAGCCTGT TCTCAGAAAC AAATCAAACC ACGACCTTAT 
CCATCAAAGG AACCGTTACC ACAGAAGCTG GTACAAGTGG GAGCATTAGA AGTACAGAAC 
GTAAAAAGAG CCATTAATCG TTATGGCACA CTACCAAAAG GTGCTAGGAT CGGAGCATAC 
CTAGAATCTT TGCGTCAAAG TGGTATGCCA TCGAATCAAG AATCTACAAC GGTGGTTTCT 
TCCATTGCAC CTAGTTCAGT AGAACAACAT GAGACTTCTA TGGACAATTC GCAACATAGA 
TCTCTCTCTC CTCGTCAAAG CAATCTACGA AATCAACCGC AAATGACTCG CAGTAATTCC 
TCGAGCGGTG TTGTCAATAC CTACCAACCT CCGAATTCCC CTCGTGGCCG AGTAGTTACT 
GTAAGAAAGA ACAATCAGTC TGATGGTGTT GGTCTGAGAA CATTTCGAGT TTCAAGTAAC 
TCGAACTTCA GAACTGCAAG TCCATCAAGA TCCGTTCAAC CATCTTTAGC GGATCTAGAG 
TTTCCGCCCC CACCCGCAGA TTTGCCTCCT CCCCCTGACG AAACTTTCTC TGGTCAAGAA 
CAAGCTGAAC TTCCGCCTCC CGTTTCTTGT ACCGAGCTAT CTCAGATAAG AAATTCTCCT 
CTTTCTATAA GAAAAGCAAA GAGCACGGAT TGGCGCACGA AAGAAGAAGA TAATGAACAG 
GAAGACAGGA ACGACGTTAG TAACATAGAG CCTTCCGTCA AAGAAGCTTG TTCGAGGTTT 
GGAGTTAATT TAAGGCGCAG AGAAACTCAA GATAATTCTT GTAGAAACTC TGATAATAAA 
AGAACGGGAT TTAAATCCAG AATGGAGACA GTAGAGCCTG CTGCACCGCC AGAGGAAGCA 
CCACCTCCCC CACCGCCACC TCCGCCGCCA CTTTCTACGA ATACACCCCC CGATAGCTTC 
GAACGTAAAC CAGGCATGAA AGAAATGTTA GAATTAAAAT TGATCAATGA AATTAAACAG 
AGCGCGGAAA CAAAACATGG TACGACTACG AAAAAGACTG GAGCGACAAG CAATACTCCA 
TCCGCACCTT TAGATCCAGC GTCGCAGCTA CTCTCGGAAC TTTGTGCTAG TTTTAATATG 
GAGTCTGGAC AGAGACACGC CCAAAACGAG TACGCTGTAT CAACTTTAAA AACCAATGAC 
GTAAATCAAG AACAACAACA GCAACAAAGT CATAACGCTC ATAAGGATTC GTCGGTGTCC 
TCTCCAGTGA CCGAGTCTCT CCTGTCCAGT GGAAATGTTG GATTCAAGCT GAAAAGAGTT 
GACAAGCGGA ATAATCCGCA AAAAGAAGAA ACATCCGATG GACAAATAAT TGATTTTAAA 
GCGAGACTTC GAAAAGTTGA GAACGCAGAA AAAGAAAAAT CCTTGGAAGA GAAAAGTACC 
GTCACTGAAC AGTCTTCAGA ATCAGAAGAG CAACAAGATG ACAAACGTAG AAGCACTGGT 
AGTATCAGTA GTTTAAAGAA GCTTTGGGAG AACAAAGAAT CTTGTGACAG TCAGCCTCAT 
AGTCCAAAAC TCAATGTCAG AGGAAGTGGT GGTAAACAAG AAATGATAGA CCAGACAGAA 
GACTCACCGG AGGATCATAG TGGAGCTTCG ACACGTAGTC AGAGTTCCGG TAGCAAAAGT 
GACGCTAGAC TATGGCCACC GCCCGAACCT GAAAAACCGG CAGTTCCAGC AAAGCCATTA 
AAACCACTAT GTTCATCAAC GAAGCACTTT GGTTCTTCGA TTTACGCAAC GCCAAACTGT 
ACGAAATCTC AGCACGCTGA GGACGACCTA AACAAACAGA ATACAGATTC AAAGACTGCG 
AAACAGGCAG TATTGGAGCT TTCGACGTTG ATTGAGAGCA GCATATTAAA CTTGAAAAGT 
AGTCCAACAA TTGTAATGAC AAGTTGGCTT CAGCTCTCTG ACAAGGTAGG GCTACTACAC 
GGAATGTGCA CGAATCTCGC AGACAGTGGT ATCGCACCAC ATGCGCGGTT TCAATTTCGC 
GAACTTCTTG CAAGATTAGA ACTTCAGGCA CGGCAGCTTC GTGCAGCCGG TATTCGGAAC 
GTTGCAGAGA ATACAAGGCT TCTCACTGAT CTTCAAAACA CGATAAAGGA TGTTGTTAAT 
GCTGTGCAAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100878636 ( XM_012286440.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012141830.1 Megachile rotundata tyrosine-protein kinase Abl (LOC100878636), transcript variant X7, mRNA.

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CloneID	OMe105648
Clone ID Related Accession (Same CDS sequence)	XM_012286440.1
Accession Version	XM_012286440.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4224bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	tyrosine-protein kinase Abl isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797180.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGAAG CTCTCTTACA AAGCAGACCG CTACCTCACA TTCCGGCATT ACCAGATGGC 
GATCCTCCTA GTGGCTCTGC TATCCAACCA ATTTCACAAC AAGTGAATAT TCAACAACAT 
GCTGGTGTTT CATCTACAGG GCTTTTGGAA GCTGCAAACA GGTGGACAAG TAAAGAAAAC 
TTATTAGCAC AGGAGGAAGA TGATCCACAA CTATTTGTGG CTCTATATGA TTTTCAAGCA 
GGTGGAGAAA ACCAACTTAG TCTTAAAAAG GGAGAGCAAG TTCGTATTCT AAGTTATAAT 
AAAAGTGGAG AATGGTGTGA AGCTCATTCA AGTACTGGTC AAGTGGGGTG GGTTCCTTCA 
AATTATGTCA CCCCTGTAAA TTCTTTGGAA AAACATTCTT GGTATCATGG AAGGATATCC 
AGAAATGCTG CTGAATATCT CCTAAGTTCT GGTATAAATG GTAGCTTTCT GGTTCGTGAA 
TCAGAAAGCA GTCCAGGTCA ACGCAGTATT TCTTTGAGAT ATGAGGGTAG AGTCTATCAT 
TATAGGATTA ATGAGGATAG TGAAGGAAAG ATGTTTGTCA CAACAGAAAG CAAATTTAAT 
ACTCTGGCAG AATTGGTACA TCATCATTCT ATGCTTGCTG ATGGTTTAAT CACACAGCTA 
CTTTATCCTG CACCAAAGCA TAACAAACCA ACTGTTTTTC CACTTAGTCC AGAACCAGAT 
GAATGGGAGA TTAATAGGAC AGATATAGTT ATGAGGCATA AGTTAGGTGG AGGACAATAT 
GGAGATGTCT ATGAAGCAGT ATGGAAAAGA TATAACATGA CTGTGGCTGT AAAAACATTA 
AAAGAAGACA CAATGGCCCT AAAGGACTTT TTGGAGGAAG CTGCAATCAT GAAGGAGATG 
AAGCATAGGA ATTTAGTTCA GCTACTGGGT GTATGTACTC GAGAACCACC TTTCTACATC 
ATTACAGAGT TTATGAGCAA AGGAAATCTG CTAGACTATT TACGGAACGA GAGTAAACAT 
CAAATCAATG CTGTCGTTTT GATGCACATG GCCACACAGA TTGCTAGTGG AATGAGTTAT 
TTGGAGAGTA GAAATTTTAT ACACAGAGAT CTAGCAGCTC GAAACTGCCT AGTGGGTGAA 
AACCATCTGG TAAAGGTCGC AGACTTTGGC TTGGCCCGGT TGATGAGAGA TGATACGTAT 
ACGGCTCACG CTGGAGCAAA GTTTCCAATA AAATGGACTG CTCCAGAAGG ATTAGCTTAC 
AATAAATTCT CTACGAAGTC TGATGTGTGG GCATTTGGAA TTTTACTTTG GGAGATAGCA 
ACCTATGGCA TGTCCCCATA CCCTGGTGTT GATTTGACGG ATGTGTATCA CATGTTGGAG 
AAAGGCTACC GAATGGAATG TCCACCTGGA TGTCCACCGA AGGTATATGA ACTGATGCGT 
CAATGCTGGC AGTGGGCTGC TGCTGACCGG CCTACCTTCA AAGAGATTCA TCATTCTCTT 
GAAAATATGT TCCAAGAATC CAGTATTACC GAGGAAGTTG AAAAACAACT GCAAGGTGGC 
GGCGATATCC CTTTACTTTC ATATAAAAAA TCTCAAACTG GTAGTACTGG AAACATACAT 
GGGCTTGTTC TAGTCTCCGA GCCATTACCC TCTTCAGAAA CTACTAGCTC GGTAACAAAA 
CTGAGTACGT TTACGGGTGG TCTATCGAGT AAAAATAACA GCAATATCGT CCAAATGAGG 
CGTTCTACGA ATAAGAAAGG AAAACAAGCT CCGGCACCTC CAAAGAGAAC GAGCCTGCTG 
TCGTCGTGCA GTTCGTTTCG CGATTCTGCC TACCAAGAGC AAGATACCCA AAATACTGAA 
ACAAACACCA TGACTCTCGA CGATGCCACG GATCTAAATG GTATTGATAA GATTCTCGAA 
GGTATTGCGC GAGATCTCGC GACGATGGCT CAAGGAACGA TTGCTGCAGG AGGCTGCGAA 
GTCGAGGAGG ATGGAGAGGG TTCTCAAGGT ACGGCGGAAC CAAATTTTAT CCCTCAACCT 
TCGACGTCCC CGGAGCCTAT ACCTGGACTA GTAGCCTGTT CTCAGAAACA AATCAAACCA 
CGACCTTATC CATCAAAGGA ACCGTTACCA CAGAAGCTGG TACAAGTGGG AGCATTAGAA 
GTACAGAACG TAAAAAGAGC CATTAATCGT TATGGCACAC TACCAAAAGG TGCTAGGATC 
GGAGCATACC TAGAATCTTT GCGTCAAAGT GGTATGCCAT CGAATCAAGA ATCTACAACG 
GTGGTTTCTT CCATTGCACC TAGTTCAGTA GAACAACATG AGACTTCTAT GGACAATTCG 
CAACATAGAT CTCTCTCTCC TCGTCAAAGC AATCTACGAA ATCAACCGCA AATGACTCGC 
AGTAATTCCT CGAGCGGTGT TGTCAATACC TACCAACCTC CGAATTCCCC TCGTGGCCGA 
GTAGTTACTG TAAGAAAGAA CAATCAGTCT GATGGTGTTG GTCTGAGAAC ATTTCGAGTT 
TCAAGTAACT CGAACTTCAG AACTGCAAGT CCATCAAGAT CCGTTCAACC ATCTTTAGCG 
GATCTAGAGT TTCCGCCCCC ACCCGCAGAT TTGCCTCCTC CCCCTGACGA AACTTTCTCT 
GGTCAAGAAC AAGCTGAACT TCCGCCTCCC GTTTCTTGTA CCGAGCTATC TCAGATAAGA 
AATTCTCCTC TTTCTATAAG AAAAGCAAAG AGCACGGATT GGCGCACGAA AGAAGAAGAT 
AATGAACAGG AAGACAGGAA CGACGTTAGT AACATAGAGC CTTCCGTCAA AGAAGCTTGT 
TCGAGGTTTG GAGTTAATTT AAGGCGCAGA GAAACTCAAG ATAATTCTTG TAGAAACTCT 
GATAATAAAA GAACGGGATT TAAATCCAGA ATGGAGACAG TAGAGCCTGC TGCACCGCCA 
GAGGAAGCAC CACCTCCCCC ACCGCCACCT CCGCCGCCAC TTTCTACGAA TACACCCCCC 
GATAGCTTCG AACGTAAACC AGGCATGAAA GAAATGTTAG AATTAAAATT GATCAATGAA 
ATTAAACAGA GCGCGGAAAC AAAACATGGT ACGACTACGA AAAAGACTGG AGCGACAAGC 
AATACTCCAT CCGCACCTTT AGATCCAGCG TCGCAGCTAC TCTCGGAACT TTGTGCTAGT 
TTTAATATGG AGTCTGGACA GAGACACGCC CAAAACGAGT ACGCTGTATC AACTTTAAAA 
ACCAATGACG TAAATCAAGA ACAACAACAG CAACAAAGTC ATAACGCTCA TAAGGATTCG 
TCGGTGTCCT CTCCAGTGAC CGAGTCTCTC CTGTCCAGTG GAAATGTTGG ATTCAAGCTG 
AAAAGAGTTG ACAAGCGGAA TAATCCGCAA AAAGAAGAAA CATCCGATGG ACAAATAATT 
GATTTTAAAG CGAGACTTCG AAAAGTTGAG AACGCAGAAA AAGAAAAATC CTTGGAAGAG 
AAAAGTACCG TCACTGAACA GTCTTCAGAA TCAGAAGAGC AACAAGATGA CAAACGTAGA 
AGCACTGGTA GTATCAGTAG TTTAAAGAAG CTTTGGGAGA ACAAAGAATC TTGTGACAGT 
CAGCCTCATA GTCCAAAACT CAATGTCAGA GGAAGTGGTG GTAAACAAGA AATGATAGAC 
CAGACAGAAG ACTCACCGGA GGATCATAGT GGAGCTTCGA CACGTAGTCA GAGTTCCGGT 
AGCAAAAGTG ACGCTAGACT ATGGCCACCG CCCGAACCTG AAAAACCGGC AGTTCCAGCA 
AAGCCATTAA AACCACTATG TTCATCAACG AAGCACTTTG GTTCTTCGAT TTACGCAACG 
CCAAACTGTA CGAAATCTCA GCACGCTGAG GACGACCTAA ACAAACAGAA TACAGATTCA 
AAGACTGCGA AACAGGCAGT ATTGGAGCTT TCGACGTTGA TTGAGAGCAG CATATTAAAC 
TTGAAAAGTA GTCCAACAAT TGTAATGACA AGTTGGCTTC AGCTCTCTGA CAAGGTAGGG 
CTACTACACG GAATGTGCAC GAATCTCGCA GACAGTGGTA TCGCACCACA TGCGCGGTTT 
CAATTTCGCG AACTTCTTGC AAGATTAGAA CTTCAGGCAC GGCAGCTTCG TGCAGCCGGT 
ATTCGGAACG TTGCAGAGAA TACAAGGCTT CTCACTGATC TTCAAAACAC GATAAAGGAT 
GTTGTTAATG CTGTGCAAAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012141830.1
CDS	297..4520
Translation	MNEALLQSRPLPHIPALPDGDPPSGSAIQPISQQVNIQQHAGVSSTGLLEAANRWTSKENLLAQEEDDPQLFVALYDFQAGGENQLSLKKGEQVRILSYNKSGEWCEAHSSTGQVGWVPSNYVTPVNSLEKHSWYHGRISRNAAEYLLSSGINGSFLVRESESSPGQRSISLRYEGRVYHYRINEDSEGKMFVTTESKFNTLAELVHHHSMLADGLITQLLYPAPKHNKPTVFPLSPEPDEWEINRTDIVMRHKLGGGQYGDVYEAVWKRYNMTVAVKTLKEDTMALKDFLEEAAIMKEMKHRNLVQLLGVCTREPPFYIITEFMSKGNLLDYLRNESKHQINAVVLMHMATQIASGMSYLESRNFIHRDLAARNCLVGENHLVKVADFGLARLMRDDTYTAHAGAKFPIKWTAPEGLAYNKFSTKSDVWAFGILLWEIATYGMSPYPGVDLTDVYHMLEKGYRMECPPGCPPKVYELMRQCWQWAAADRPTFKEIHHSLENMFQESSITEEVEKQLQGGGDIPLLSYKKSQTGSTGNIHGLVLVSEPLPSSETTSSVTKLSTFTGGLSSKNNSNIVQMRRSTNKKGKQAPAPPKRTSLLSSCSSFRDSAYQEQDTQNTETNTMTLDDATDLNGIDKILEGIARDLATMAQGTIAAGGCEVEEDGEGSQGTAEPNFIPQPSTSPEPIPGLVACSQKQIKPRPYPSKEPLPQKLVQVGALEVQNVKRAINRYGTLPKGARIGAYLESLRQSGMPSNQESTTVVSSIAPSSVEQHETSMDNSQHRSLSPRQSNLRNQPQMTRSNSSSGVVNTYQPPNSPRGRVVTVRKNNQSDGVGLRTFRVSSNSNFRTASPSRSVQPSLADLEFPPPPADLPPPPDETFSGQEQAELPPPVSCTELSQIRNSPLSIRKAKSTDWRTKEEDNEQEDRNDVSNIEPSVKEACSRFGVNLRRRETQDNSCRNSDNKRTGFKSRMETVEPAAPPEEAPPPPPPPPPPLSTNTPPDSFERKPGMKEMLELKLINEIKQSAETKHGTTTKKTGATSNTPSAPLDPASQLLSELCASFNMESGQRHAQNEYAVSTLKTNDVNQEQQQQQSHNAHKDSSVSSPVTESLLSSGNVGFKLKRVDKRNNPQKEETSDGQIIDFKARLRKVENAEKEKSLEEKSTVTEQSSESEEQQDDKRRSTGSISSLKKLWENKESCDSQPHSPKLNVRGSGGKQEMIDQTEDSPEDHSGASTRSQSSGSKSDARLWPPPEPEKPAVPAKPLKPLCSSTKHFGSSIYATPNCTKSQHAEDDLNKQNTDSKTAKQAVLELSTLIESSILNLKSSPTIVMTSWLQLSDKVGLLHGMCTNLADSGIAPHARFQFRELLARLELQARQLRAAGIRNVAENTRLLTDLQNTIKDVVNAVQR

Target ORF information:

RefSeq Version	XM_012286440.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata tyrosine-protein kinase Abl (LOC100878636), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012286440.1

ORF Insert Sequence:

ATGAATGAAG CTCTCTTACA AAGCAGACCG CTACCTCACA TTCCGGCATT ACCAGATGGC 
GATCCTCCTA GTGGCTCTGC TATCCAACCA ATTTCACAAC AAGTGAATAT TCAACAACAT 
GCTGGTGTTT CATCTACAGG GCTTTTGGAA GCTGCAAACA GGTGGACAAG TAAAGAAAAC 
TTATTAGCAC AGGAGGAAGA TGATCCACAA CTATTTGTGG CTCTATATGA TTTTCAAGCA 
GGTGGAGAAA ACCAACTTAG TCTTAAAAAG GGAGAGCAAG TTCGTATTCT AAGTTATAAT 
AAAAGTGGAG AATGGTGTGA AGCTCATTCA AGTACTGGTC AAGTGGGGTG GGTTCCTTCA 
AATTATGTCA CCCCTGTAAA TTCTTTGGAA AAACATTCTT GGTATCATGG AAGGATATCC 
AGAAATGCTG CTGAATATCT CCTAAGTTCT GGTATAAATG GTAGCTTTCT GGTTCGTGAA 
TCAGAAAGCA GTCCAGGTCA ACGCAGTATT TCTTTGAGAT ATGAGGGTAG AGTCTATCAT 
TATAGGATTA ATGAGGATAG TGAAGGAAAG ATGTTTGTCA CAACAGAAAG CAAATTTAAT 
ACTCTGGCAG AATTGGTACA TCATCATTCT ATGCTTGCTG ATGGTTTAAT CACACAGCTA 
CTTTATCCTG CACCAAAGCA TAACAAACCA ACTGTTTTTC CACTTAGTCC AGAACCAGAT 
GAATGGGAGA TTAATAGGAC AGATATAGTT ATGAGGCATA AGTTAGGTGG AGGACAATAT 
GGAGATGTCT ATGAAGCAGT ATGGAAAAGA TATAACATGA CTGTGGCTGT AAAAACATTA 
AAAGAAGACA CAATGGCCCT AAAGGACTTT TTGGAGGAAG CTGCAATCAT GAAGGAGATG 
AAGCATAGGA ATTTAGTTCA GCTACTGGGT GTATGTACTC GAGAACCACC TTTCTACATC 
ATTACAGAGT TTATGAGCAA AGGAAATCTG CTAGACTATT TACGGAACGA GAGTAAACAT 
CAAATCAATG CTGTCGTTTT GATGCACATG GCCACACAGA TTGCTAGTGG AATGAGTTAT 
TTGGAGAGTA GAAATTTTAT ACACAGAGAT CTAGCAGCTC GAAACTGCCT AGTGGGTGAA 
AACCATCTGG TAAAGGTCGC AGACTTTGGC TTGGCCCGGT TGATGAGAGA TGATACGTAT 
ACGGCTCACG CTGGAGCAAA GTTTCCAATA AAATGGACTG CTCCAGAAGG ATTAGCTTAC 
AATAAATTCT CTACGAAGTC TGATGTGTGG GCATTTGGAA TTTTACTTTG GGAGATAGCA 
ACCTATGGCA TGTCCCCATA CCCTGGTGTT GATTTGACGG ATGTGTATCA CATGTTGGAG 
AAAGGCTACC GAATGGAATG TCCACCTGGA TGTCCACCGA AGGTATATGA ACTGATGCGT 
CAATGCTGGC AGTGGGCTGC TGCTGACCGG CCTACCTTCA AAGAGATTCA TCATTCTCTT 
GAAAATATGT TCCAAGAATC CAGTATTACC GAGGAAGTTG AAAAACAACT GCAAGGTGGC 
GGCGATATCC CTTTACTTTC ATATAAAAAA TCTCAAACTG GTAGTACTGG AAACATACAT 
GGGCTTGTTC TAGTCTCCGA GCCATTACCC TCTTCAGAAA CTACTAGCTC GGTAACAAAA 
CTGAGTACGT TTACGGGTGG TCTATCGAGT AAAAATAACA GCAATATCGT CCAAATGAGG 
CGTTCTACGA ATAAGAAAGG AAAACAAGCT CCGGCACCTC CAAAGAGAAC GAGCCTGCTG 
TCGTCGTGCA GTTCGTTTCG CGATTCTGCC TACCAAGAGC AAGATACCCA AAATACTGAA 
ACAAACACCA TGACTCTCGA CGATGCCACG GATCTAAATG GTATTGATAA GATTCTCGAA 
GGTATTGCGC GAGATCTCGC GACGATGGCT CAAGGAACGA TTGCTGCAGG AGGCTGCGAA 
GTCGAGGAGG ATGGAGAGGG TTCTCAAGGT ACGGCGGAAC CAAATTTTAT CCCTCAACCT 
TCGACGTCCC CGGAGCCTAT ACCTGGACTA GTAGCCTGTT CTCAGAAACA AATCAAACCA 
CGACCTTATC CATCAAAGGA ACCGTTACCA CAGAAGCTGG TACAAGTGGG AGCATTAGAA 
GTACAGAACG TAAAAAGAGC CATTAATCGT TATGGCACAC TACCAAAAGG TGCTAGGATC 
GGAGCATACC TAGAATCTTT GCGTCAAAGT GGTATGCCAT CGAATCAAGA ATCTACAACG 
GTGGTTTCTT CCATTGCACC TAGTTCAGTA GAACAACATG AGACTTCTAT GGACAATTCG 
CAACATAGAT CTCTCTCTCC TCGTCAAAGC AATCTACGAA ATCAACCGCA AATGACTCGC 
AGTAATTCCT CGAGCGGTGT TGTCAATACC TACCAACCTC CGAATTCCCC TCGTGGCCGA 
GTAGTTACTG TAAGAAAGAA CAATCAGTCT GATGGTGTTG GTCTGAGAAC ATTTCGAGTT 
TCAAGTAACT CGAACTTCAG AACTGCAAGT CCATCAAGAT CCGTTCAACC ATCTTTAGCG 
GATCTAGAGT TTCCGCCCCC ACCCGCAGAT TTGCCTCCTC CCCCTGACGA AACTTTCTCT 
GGTCAAGAAC AAGCTGAACT TCCGCCTCCC GTTTCTTGTA CCGAGCTATC TCAGATAAGA 
AATTCTCCTC TTTCTATAAG AAAAGCAAAG AGCACGGATT GGCGCACGAA AGAAGAAGAT 
AATGAACAGG AAGACAGGAA CGACGTTAGT AACATAGAGC CTTCCGTCAA AGAAGCTTGT 
TCGAGGTTTG GAGTTAATTT AAGGCGCAGA GAAACTCAAG ATAATTCTTG TAGAAACTCT 
GATAATAAAA GAACGGGATT TAAATCCAGA ATGGAGACAG TAGAGCCTGC TGCACCGCCA 
GAGGAAGCAC CACCTCCCCC ACCGCCACCT CCGCCGCCAC TTTCTACGAA TACACCCCCC 
GATAGCTTCG AACGTAAACC AGGCATGAAA GAAATGTTAG AATTAAAATT GATCAATGAA 
ATTAAACAGA GCGCGGAAAC AAAACATGGT ACGACTACGA AAAAGACTGG AGCGACAAGC 
AATACTCCAT CCGCACCTTT AGATCCAGCG TCGCAGCTAC TCTCGGAACT TTGTGCTAGT 
TTTAATATGG AGTCTGGACA GAGACACGCC CAAAACGAGT ACGCTGTATC AACTTTAAAA 
ACCAATGACG TAAATCAAGA ACAACAACAG CAACAAAGTC ATAACGCTCA TAAGGATTCG 
TCGGTGTCCT CTCCAGTGAC CGAGTCTCTC CTGTCCAGTG GAAATGTTGG ATTCAAGCTG 
AAAAGAGTTG ACAAGCGGAA TAATCCGCAA AAAGAAGAAA CATCCGATGG ACAAATAATT 
GATTTTAAAG CGAGACTTCG AAAAGTTGAG AACGCAGAAA AAGAAAAATC CTTGGAAGAG 
AAAAGTACCG TCACTGAACA GTCTTCAGAA TCAGAAGAGC AACAAGATGA CAAACGTAGA 
AGCACTGGTA GTATCAGTAG TTTAAAGAAG CTTTGGGAGA ACAAAGAATC TTGTGACAGT 
CAGCCTCATA GTCCAAAACT CAATGTCAGA GGAAGTGGTG GTAAACAAGA AATGATAGAC 
CAGACAGAAG ACTCACCGGA GGATCATAGT GGAGCTTCGA CACGTAGTCA GAGTTCCGGT 
AGCAAAAGTG ACGCTAGACT ATGGCCACCG CCCGAACCTG AAAAACCGGC AGTTCCAGCA 
AAGCCATTAA AACCACTATG TTCATCAACG AAGCACTTTG GTTCTTCGAT TTACGCAACG 
CCAAACTGTA CGAAATCTCA GCACGCTGAG GACGACCTAA ACAAACAGAA TACAGATTCA 
AAGACTGCGA AACAGGCAGT ATTGGAGCTT TCGACGTTGA TTGAGAGCAG CATATTAAAC 
TTGAAAAGTA GTCCAACAAT TGTAATGACA AGTTGGCTTC AGCTCTCTGA CAAGGTAGGG 
CTACTACACG GAATGTGCAC GAATCTCGCA GACAGTGGTA TCGCACCACA TGCGCGGTTT 
CAATTTCGCG AACTTCTTGC AAGATTAGAA CTTCAGGCAC GGCAGCTTCG TGCAGCCGGT 
ATTCGGAACG TTGCAGAGAA TACAAGGCTT CTCACTGATC TTCAAAACAC GATAAAGGAT 
GTTGTTAATG CTGTGCAAAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100878636 ( XM_012286441.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012141831.1 Megachile rotundata tyrosine-protein kinase Abl (LOC100878636), transcript variant X8, mRNA.

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CloneID	OMe105649
Clone ID Related Accession (Same CDS sequence)	XM_012286441.1
Accession Version	XM_012286441.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	tyrosine-protein kinase Abl isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797180.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGCTC TCTTACAAAG CAGACCGCTA CCTCACATTC CGGCATTACC AGATGGCGAT 
CCTCCTAGTG GCTCTGCTAT CCAACCAATT TCACAACAAG TGAATATTCA ACAACATGCT 
GGTGTTTCAT CTACAGGGCT TTTGGAAGCT GCAAACAGGT GGACAAGTAA AGAAAACTTA 
TTAGCACAGG AGGAAGATGA TCCACAACTA TTTGTGGCTC TATATGATTT TCAAGCAGGT 
GGAGAAAACC AACTTAGTCT TAAAAAGGGA GAGCAAGTTC GTATTCTAAG TTATAATAAA 
AGTGGAGAAT GGTGTGAAGC TCATTCAAGT ACTGGTCAAG TGGGGTGGGT TCCTTCAAAT 
TATGTCACCC CTGTAAATTC TTTGGAAAAA CATTCTTGGT ATCATGGAAG GATATCCAGA 
AATGCTGCTG AATATCTCCT AAGTTCTGGT ATAAATGGTA GCTTTCTGGT TCGTGAATCA 
GAAAGCAGTC CAGGTCAACG CAGTATTTCT TTGAGATATG AGGGTAGAGT CTATCATTAT 
AGGATTAATG AGGATAGTGA AGGAAAGATG TTTGTCACAA CAGAAAGCAA ATTTAATACT 
CTGGCAGAAT TGGTACATCA TCATTCTATG CTTGCTGATG GTTTAATCAC ACAGCTACTT 
TATCCTGCAC CAAAGCATAA CAAACCAACT GTTTTTCCAC TTAGTCCAGA ACCAGATGAA 
TGGGAGATTA ATAGGACAGA TATAGTTATG AGGCATAAGT TAGGTGGAGG ACAATATGGA 
GATGTCTATG AAGCAGTATG GAAAAGATAT AACATGACTG TGGCTGTAAA AACATTAAAA 
GAAGACACAA TGGCCCTAAA GGACTTTTTG GAGGAAGCTG CAATCATGAA GGAGATGAAG 
CATAGGAATT TAGTTCAGCT ACTGGGTGTA TGTACTCGAG AACCACCTTT CTACATCATT 
ACAGAGTTTA TGAGCAAAGG AAATCTGCTA GACTATTTAC GGAACGAGAG TAAACATCAA 
ATCAATGCTG TCGTTTTGAT GCACATGGCC ACACAGATTG CTAGTGGAAT GAGTTATTTG 
GAGAGTAGAA ATTTTATACA CAGAGATCTA GCAGCTCGAA ACTGCCTAGT GGGTGAAAAC 
CATCTGGTAA AGGTCGCAGA CTTTGGCTTG GCCCGGTTGA TGAGAGATGA TACGTATACG 
GCTCACGCTG GAGCAAAGTT TCCAATAAAA TGGACTGCTC CAGAAGGATT AGCTTACAAT 
AAATTCTCTA CGAAGTCTGA TGTGTGGGCA TTTGGAATTT TACTTTGGGA GATAGCAACC 
TATGGCATGT CCCCATACCC TGGTGTTGAT TTGACGGATG TGTATCACAT GTTGGAGAAA 
GGCTACCGAA TGGAATGTCC ACCTGGATGT CCACCGAAGG TATATGAACT GATGCGTCAA 
TGCTGGCAGT GGGCTGCTGC TGACCGGCCT ACCTTCAAAG AGATTCATCA TTCTCTTGAA 
AATATGTTCC AAGAATCCAG TATTACCGAG GAAGTTGAAA AACAACTGCA AGGTGGCGGC 
GATATCCCTT TACTTTCATA TAAAAAATCT CAAACTGGTA GTACTGGAAA CATACATGGG 
CTTGTTCTAG TCTCCGAGCC ATTACCCTCT TCAGAAACTA CTAGCTCGGT AACAAAACTG 
AGTACGTTTA CGGGTGGTCT ATCGAGTAAA AATAACAGCA ATATCGTCCA AATGAGGCGT 
TCTACGAATA AGAAAGGAAA ACAAGCTCCG GCACCTCCAA AGAGAACGAG CCTGCTGTCG 
TCGTGCAGTT CGTTTCGCGA TTCTGCCTAC CAAGAGCAAG ATACCCAAAA TACTGAAACA 
AACACCATGA CTCTCGACGA TGCCACGGAT CTAAATGGTA TTGATAAGAT TCTCGAAGGT 
ATTGCGCGAG ATCTCGCGAC GATGGCTCAA GGAACGATTG CTGCAGGAGG CTGCGAAGTC 
GAGGAGGATG GAGAGGGTTC TCAAGGTACG GCGGAACCAA ATTTTATCCC TCAACCTTCG 
ACGTCCCCGG AGCCTATACC TGGACTAGTA GCCTGTTCTC AGAAACAAAT CAAACCACGA 
CCTTATCCAT CAAAGGAACC GTTACCACAG AAGCTGGTAC AAGTGGGAGC ATTAGAAGTA 
CAGAACGTAA AAAGAGCCAT TAATCGTTAT GGCACACTAC CAAAAGGTGC TAGGATCGGA 
GCATACCTAG AATCTTTGCG TCAAAGTGGT ATGCCATCGA ATCAAGAATC TACAACGGTG 
GTTTCTTCCA TTGCACCTAG TTCAGTAGAA CAACATGAGA CTTCTATGGA CAATTCGCAA 
CATAGATCTC TCTCTCCTCG TCAAAGCAAT CTACGAAATC AACCGCAAAT GACTCGCAGT 
AATTCCTCGA GCGGTGTTGT CAATACCTAC CAACCTCCGA ATTCCCCTCG TGGCCGAGTA 
GTTACTGTAA GAAAGAACAA TCAGTCTGAT GGTGTTGGTC TGAGAACATT TCGAGTTTCA 
AGTAACTCGA ACTTCAGAAC TGCAAGTCCA TCAAGATCCG TTCAACCATC TTTAGCGGAT 
CTAGAGTTTC CGCCCCCACC CGCAGATTTG CCTCCTCCCC CTGACGAAAC TTTCTCTGGT 
CAAGAACAAG CTGAACTTCC GCCTCCCGTT TCTTGTACCG AGCTATCTCA GATAAGAAAT 
TCTCCTCTTT CTATAAGAAA AGCAAAGAGC ACGGATTGGC GCACGAAAGA AGAAGATAAT 
GAACAGGAAG ACAGGAACGA CGTTAGTAAC ATAGAGCCTT CCGTCAAAGA AGCTTGTTCG 
AGGTTTGGAG TTAATTTAAG GCGCAGAGAA ACTCAAGATA ATTCTTGTAG AAACTCTGAT 
AATAAAAGAA CGGGATTTAA ATCCAGAATG GAGACAGTAG AGCCTGCTGC ACCGCCAGAG 
GAAGCACCAC CTCCCCCACC GCCACCTCCG CCGCCACTTT CTACGAATAC ACCCCCCGAT 
AGCTTCGAAC GTAAACCAGG CATGAAAGAA ATGTTAGAAT TAAAATTGAT CAATGAAATT 
AAACAGAGCG CGGAAACAAA ACATGGTACG ACTACGAAAA AGACTGGAGC GACAAGCAAT 
ACTCCATCCG CACCTTTAGA TCCAGCGTCG CAGCTACTCT CGGAACTTTG TGCTAGTTTT 
AATATGGAGT CTGGACAGAG ACACGCCCAA AACGAGTACG CTGTATCAAC TTTAAAAACC 
AATGACGTAA ATCAAGAACA ACAACAGCAA CAAAGTCATA ACGCTCATAA GGATTCGTCG 
GTGTCCTCTC CAGTGACCGA GTCTCTCCTG TCCAGTGGAA ATGTTGGATT CAAGCTGAAA 
AGAGTTGACA AGCGGAATAA TCCGCAAAAA GAAGAAACAT CCGATGGACA AATAATTGAT 
TTTAAAGCGA GACTTCGAAA AGTTGAGAAC GCAGAAAAAG AAAAATCCTT GGAAGAGAAA 
AGTACCGTCA CTGAACAGTC TTCAGAATCA GAAGAGCAAC AAGATGACAA ACGTAGAAGC 
ACTGGTAGTA TCAGTAGTTT AAAGAAGCTT TGGGAGAACA AAGAATCTTG TGACAGTCAG 
CCTCATAGTC CAAAACTCAA TGTCAGAGGA AGTGGTGGTA AACAAGAAAT GATAGACCAG 
ACAGAAGACT CACCGGAGGA TCATAGTGGA GCTTCGACAC GTAGTCAGAG TTCCGGTAGC 
AAAAGTGACG CTAGACTATG GCCACCGCCC GAACCTGAAA AACCGGCAGT TCCAGCAAAG 
CCATTAAAAC CACTATGTTC ATCAACGAAG CACTTTGGTT CTTCGATTTA CGCAACGCCA 
AACTGTACGA AATCTCAGCA CGCTGAGGAC GACCTAAACA AACAGAATAC AGATTCAAAG 
ACTGCGAAAC AGGCAGTATT GGAGCTTTCG ACGTTGATTG AGAGCAGCAT ATTAAACTTG 
AAAAGTAGTC CAACAATTGT AATGACAAGT TGGCTTCAGC TCTCTGACAA GGTAGGGCTA 
CTACACGGAA TGTGCACGAA TCTCGCAGAC AGTGGTATCG CACCACATGC GCGGTTTCAA 
TTTCGCGAAC TTCTTGCAAG ATTAGAACTT CAGGCACGGC AGCTTCGTGC AGCCGGTATT 
CGGAACGTTG CAGAGAATAC AAGGCTTCTC ACTGATCTTC AAAACACGAT AAAGGATGTT 
GTTAATGCTG TGCAAAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012141831.1
CDS	297..4517
Translation	MNALLQSRPLPHIPALPDGDPPSGSAIQPISQQVNIQQHAGVSSTGLLEAANRWTSKENLLAQEEDDPQLFVALYDFQAGGENQLSLKKGEQVRILSYNKSGEWCEAHSSTGQVGWVPSNYVTPVNSLEKHSWYHGRISRNAAEYLLSSGINGSFLVRESESSPGQRSISLRYEGRVYHYRINEDSEGKMFVTTESKFNTLAELVHHHSMLADGLITQLLYPAPKHNKPTVFPLSPEPDEWEINRTDIVMRHKLGGGQYGDVYEAVWKRYNMTVAVKTLKEDTMALKDFLEEAAIMKEMKHRNLVQLLGVCTREPPFYIITEFMSKGNLLDYLRNESKHQINAVVLMHMATQIASGMSYLESRNFIHRDLAARNCLVGENHLVKVADFGLARLMRDDTYTAHAGAKFPIKWTAPEGLAYNKFSTKSDVWAFGILLWEIATYGMSPYPGVDLTDVYHMLEKGYRMECPPGCPPKVYELMRQCWQWAAADRPTFKEIHHSLENMFQESSITEEVEKQLQGGGDIPLLSYKKSQTGSTGNIHGLVLVSEPLPSSETTSSVTKLSTFTGGLSSKNNSNIVQMRRSTNKKGKQAPAPPKRTSLLSSCSSFRDSAYQEQDTQNTETNTMTLDDATDLNGIDKILEGIARDLATMAQGTIAAGGCEVEEDGEGSQGTAEPNFIPQPSTSPEPIPGLVACSQKQIKPRPYPSKEPLPQKLVQVGALEVQNVKRAINRYGTLPKGARIGAYLESLRQSGMPSNQESTTVVSSIAPSSVEQHETSMDNSQHRSLSPRQSNLRNQPQMTRSNSSSGVVNTYQPPNSPRGRVVTVRKNNQSDGVGLRTFRVSSNSNFRTASPSRSVQPSLADLEFPPPPADLPPPPDETFSGQEQAELPPPVSCTELSQIRNSPLSIRKAKSTDWRTKEEDNEQEDRNDVSNIEPSVKEACSRFGVNLRRRETQDNSCRNSDNKRTGFKSRMETVEPAAPPEEAPPPPPPPPPPLSTNTPPDSFERKPGMKEMLELKLINEIKQSAETKHGTTTKKTGATSNTPSAPLDPASQLLSELCASFNMESGQRHAQNEYAVSTLKTNDVNQEQQQQQSHNAHKDSSVSSPVTESLLSSGNVGFKLKRVDKRNNPQKEETSDGQIIDFKARLRKVENAEKEKSLEEKSTVTEQSSESEEQQDDKRRSTGSISSLKKLWENKESCDSQPHSPKLNVRGSGGKQEMIDQTEDSPEDHSGASTRSQSSGSKSDARLWPPPEPEKPAVPAKPLKPLCSSTKHFGSSIYATPNCTKSQHAEDDLNKQNTDSKTAKQAVLELSTLIESSILNLKSSPTIVMTSWLQLSDKVGLLHGMCTNLADSGIAPHARFQFRELLARLELQARQLRAAGIRNVAENTRLLTDLQNTIKDVVNAVQR

Target ORF information:

RefSeq Version	XM_012286441.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata tyrosine-protein kinase Abl (LOC100878636), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012286441.1

ORF Insert Sequence:

ATGAATGCTC TCTTACAAAG CAGACCGCTA CCTCACATTC CGGCATTACC AGATGGCGAT 
CCTCCTAGTG GCTCTGCTAT CCAACCAATT TCACAACAAG TGAATATTCA ACAACATGCT 
GGTGTTTCAT CTACAGGGCT TTTGGAAGCT GCAAACAGGT GGACAAGTAA AGAAAACTTA 
TTAGCACAGG AGGAAGATGA TCCACAACTA TTTGTGGCTC TATATGATTT TCAAGCAGGT 
GGAGAAAACC AACTTAGTCT TAAAAAGGGA GAGCAAGTTC GTATTCTAAG TTATAATAAA 
AGTGGAGAAT GGTGTGAAGC TCATTCAAGT ACTGGTCAAG TGGGGTGGGT TCCTTCAAAT 
TATGTCACCC CTGTAAATTC TTTGGAAAAA CATTCTTGGT ATCATGGAAG GATATCCAGA 
AATGCTGCTG AATATCTCCT AAGTTCTGGT ATAAATGGTA GCTTTCTGGT TCGTGAATCA 
GAAAGCAGTC CAGGTCAACG CAGTATTTCT TTGAGATATG AGGGTAGAGT CTATCATTAT 
AGGATTAATG AGGATAGTGA AGGAAAGATG TTTGTCACAA CAGAAAGCAA ATTTAATACT 
CTGGCAGAAT TGGTACATCA TCATTCTATG CTTGCTGATG GTTTAATCAC ACAGCTACTT 
TATCCTGCAC CAAAGCATAA CAAACCAACT GTTTTTCCAC TTAGTCCAGA ACCAGATGAA 
TGGGAGATTA ATAGGACAGA TATAGTTATG AGGCATAAGT TAGGTGGAGG ACAATATGGA 
GATGTCTATG AAGCAGTATG GAAAAGATAT AACATGACTG TGGCTGTAAA AACATTAAAA 
GAAGACACAA TGGCCCTAAA GGACTTTTTG GAGGAAGCTG CAATCATGAA GGAGATGAAG 
CATAGGAATT TAGTTCAGCT ACTGGGTGTA TGTACTCGAG AACCACCTTT CTACATCATT 
ACAGAGTTTA TGAGCAAAGG AAATCTGCTA GACTATTTAC GGAACGAGAG TAAACATCAA 
ATCAATGCTG TCGTTTTGAT GCACATGGCC ACACAGATTG CTAGTGGAAT GAGTTATTTG 
GAGAGTAGAA ATTTTATACA CAGAGATCTA GCAGCTCGAA ACTGCCTAGT GGGTGAAAAC 
CATCTGGTAA AGGTCGCAGA CTTTGGCTTG GCCCGGTTGA TGAGAGATGA TACGTATACG 
GCTCACGCTG GAGCAAAGTT TCCAATAAAA TGGACTGCTC CAGAAGGATT AGCTTACAAT 
AAATTCTCTA CGAAGTCTGA TGTGTGGGCA TTTGGAATTT TACTTTGGGA GATAGCAACC 
TATGGCATGT CCCCATACCC TGGTGTTGAT TTGACGGATG TGTATCACAT GTTGGAGAAA 
GGCTACCGAA TGGAATGTCC ACCTGGATGT CCACCGAAGG TATATGAACT GATGCGTCAA 
TGCTGGCAGT GGGCTGCTGC TGACCGGCCT ACCTTCAAAG AGATTCATCA TTCTCTTGAA 
AATATGTTCC AAGAATCCAG TATTACCGAG GAAGTTGAAA AACAACTGCA AGGTGGCGGC 
GATATCCCTT TACTTTCATA TAAAAAATCT CAAACTGGTA GTACTGGAAA CATACATGGG 
CTTGTTCTAG TCTCCGAGCC ATTACCCTCT TCAGAAACTA CTAGCTCGGT AACAAAACTG 
AGTACGTTTA CGGGTGGTCT ATCGAGTAAA AATAACAGCA ATATCGTCCA AATGAGGCGT 
TCTACGAATA AGAAAGGAAA ACAAGCTCCG GCACCTCCAA AGAGAACGAG CCTGCTGTCG 
TCGTGCAGTT CGTTTCGCGA TTCTGCCTAC CAAGAGCAAG ATACCCAAAA TACTGAAACA 
AACACCATGA CTCTCGACGA TGCCACGGAT CTAAATGGTA TTGATAAGAT TCTCGAAGGT 
ATTGCGCGAG ATCTCGCGAC GATGGCTCAA GGAACGATTG CTGCAGGAGG CTGCGAAGTC 
GAGGAGGATG GAGAGGGTTC TCAAGGTACG GCGGAACCAA ATTTTATCCC TCAACCTTCG 
ACGTCCCCGG AGCCTATACC TGGACTAGTA GCCTGTTCTC AGAAACAAAT CAAACCACGA 
CCTTATCCAT CAAAGGAACC GTTACCACAG AAGCTGGTAC AAGTGGGAGC ATTAGAAGTA 
CAGAACGTAA AAAGAGCCAT TAATCGTTAT GGCACACTAC CAAAAGGTGC TAGGATCGGA 
GCATACCTAG AATCTTTGCG TCAAAGTGGT ATGCCATCGA ATCAAGAATC TACAACGGTG 
GTTTCTTCCA TTGCACCTAG TTCAGTAGAA CAACATGAGA CTTCTATGGA CAATTCGCAA 
CATAGATCTC TCTCTCCTCG TCAAAGCAAT CTACGAAATC AACCGCAAAT GACTCGCAGT 
AATTCCTCGA GCGGTGTTGT CAATACCTAC CAACCTCCGA ATTCCCCTCG TGGCCGAGTA 
GTTACTGTAA GAAAGAACAA TCAGTCTGAT GGTGTTGGTC TGAGAACATT TCGAGTTTCA 
AGTAACTCGA ACTTCAGAAC TGCAAGTCCA TCAAGATCCG TTCAACCATC TTTAGCGGAT 
CTAGAGTTTC CGCCCCCACC CGCAGATTTG CCTCCTCCCC CTGACGAAAC TTTCTCTGGT 
CAAGAACAAG CTGAACTTCC GCCTCCCGTT TCTTGTACCG AGCTATCTCA GATAAGAAAT 
TCTCCTCTTT CTATAAGAAA AGCAAAGAGC ACGGATTGGC GCACGAAAGA AGAAGATAAT 
GAACAGGAAG ACAGGAACGA CGTTAGTAAC ATAGAGCCTT CCGTCAAAGA AGCTTGTTCG 
AGGTTTGGAG TTAATTTAAG GCGCAGAGAA ACTCAAGATA ATTCTTGTAG AAACTCTGAT 
AATAAAAGAA CGGGATTTAA ATCCAGAATG GAGACAGTAG AGCCTGCTGC ACCGCCAGAG 
GAAGCACCAC CTCCCCCACC GCCACCTCCG CCGCCACTTT CTACGAATAC ACCCCCCGAT 
AGCTTCGAAC GTAAACCAGG CATGAAAGAA ATGTTAGAAT TAAAATTGAT CAATGAAATT 
AAACAGAGCG CGGAAACAAA ACATGGTACG ACTACGAAAA AGACTGGAGC GACAAGCAAT 
ACTCCATCCG CACCTTTAGA TCCAGCGTCG CAGCTACTCT CGGAACTTTG TGCTAGTTTT 
AATATGGAGT CTGGACAGAG ACACGCCCAA AACGAGTACG CTGTATCAAC TTTAAAAACC 
AATGACGTAA ATCAAGAACA ACAACAGCAA CAAAGTCATA ACGCTCATAA GGATTCGTCG 
GTGTCCTCTC CAGTGACCGA GTCTCTCCTG TCCAGTGGAA ATGTTGGATT CAAGCTGAAA 
AGAGTTGACA AGCGGAATAA TCCGCAAAAA GAAGAAACAT CCGATGGACA AATAATTGAT 
TTTAAAGCGA GACTTCGAAA AGTTGAGAAC GCAGAAAAAG AAAAATCCTT GGAAGAGAAA 
AGTACCGTCA CTGAACAGTC TTCAGAATCA GAAGAGCAAC AAGATGACAA ACGTAGAAGC 
ACTGGTAGTA TCAGTAGTTT AAAGAAGCTT TGGGAGAACA AAGAATCTTG TGACAGTCAG 
CCTCATAGTC CAAAACTCAA TGTCAGAGGA AGTGGTGGTA AACAAGAAAT GATAGACCAG 
ACAGAAGACT CACCGGAGGA TCATAGTGGA GCTTCGACAC GTAGTCAGAG TTCCGGTAGC 
AAAAGTGACG CTAGACTATG GCCACCGCCC GAACCTGAAA AACCGGCAGT TCCAGCAAAG 
CCATTAAAAC CACTATGTTC ATCAACGAAG CACTTTGGTT CTTCGATTTA CGCAACGCCA 
AACTGTACGA AATCTCAGCA CGCTGAGGAC GACCTAAACA AACAGAATAC AGATTCAAAG 
ACTGCGAAAC AGGCAGTATT GGAGCTTTCG ACGTTGATTG AGAGCAGCAT ATTAAACTTG 
AAAAGTAGTC CAACAATTGT AATGACAAGT TGGCTTCAGC TCTCTGACAA GGTAGGGCTA 
CTACACGGAA TGTGCACGAA TCTCGCAGAC AGTGGTATCG CACCACATGC GCGGTTTCAA 
TTTCGCGAAC TTCTTGCAAG ATTAGAACTT CAGGCACGGC AGCTTCGTGC AGCCGGTATT 
CGGAACGTTG CAGAGAATAC AAGGCTTCTC ACTGATCTTC AAAACACGAT AAAGGATGTT 
GTTAATGCTG TGCAAAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100878636 ( XM_012286442.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012141832.1 Megachile rotundata tyrosine-protein kinase Abl (LOC100878636), transcript variant X9, mRNA.

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CloneID	OMe105650
Clone ID Related Accession (Same CDS sequence)	XM_012286442.1
Accession Version	XM_012286442.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3924bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	tyrosine-protein kinase Abl isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797180.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGCTC AACAGACTAA GGAAAGAATT GTTCCTGCCG GCTCTACTGC GCGACAGACT 
CGCAAACAGC CAAGGAACCT CAAGGAGTCT CGTCTTGTTG GTTCCAATAT ATTTACAGAA 
CACAGCGAAG CTCTCTTACA AAGCAGACCG CTACCTCACA TTCCGGCATT ACCAGATGGC 
GATCCTCCTA GTGGCTCTGC TATCCAACCA ATTTCACAAC AAGTGAATAT TCAACAACAT 
GCTGGTGTTT CATCTACAGG GCTTTTGGAA GCTGCAAACA GGTGGACAAG TAAAGAAAAC 
TTATTAGCAC AGGAGGAAGA TGATCCACAA CTATTTGTGG CTCTATATGA TTTTCAAGCA 
GGTGGAGAAA ACCAACTTAG TCTTAAAAAG GGAGAGCAAG TTCGTATTCT AAGTTATAAT 
AAAAGTGGAG AATGGTGTGA AGCTCATTCA AGTACTGGTC AAGTGGGGTG GGTTCCTTCA 
AATTATGTCA CCCCTGTAAA TTCTTTGGAA AAACATTCTT GGTATCATGG AAGGATATCC 
AGAAATGCTG CTGAATATCT CCTAAGTTCT GGTATAAATG GTAGCTTTCT GGTTCGTGAA 
TCAGAAAGCA GTCCAGGTCA ACGCAGTATT TCTTTGAGAT ATGAGGGTAG AGTCTATCAT 
TATAGGATTA ATGAGGATAG TGAAGGAAAG ATGTTTGTCA CAACAGAAAG CAAATTTAAT 
ACTCTGGCAG AATTGGTACA TCATCATTCT ATGCTTGCTG ATGGTTTAAT CACACAGCTA 
CTTTATCCTG CACCAAAGCA TAACAAACCA ACTGTTTTTC CACTTAGTCC AGAACCAGAT 
GAATGGGAGA TTAATAGGAC AGATATAGTT ATGAGGCATA AGTTAGGTGG AGGACAATAT 
GGAGATGTCT ATGAAGCAGT ATGGAAAAGA TATAACATGA CTGTGGCTGT AAAAACATTA 
AAAGAAGACA CAATGGCCCT AAAGGACTTT TTGGAGGAAG CTGCAATCAT GAAGGAGATG 
AAGCATAGGA ATTTAGTTCA GCTACTGGGT GTATGTACTC GAGAACCACC TTTCTACATC 
ATTACAGAGT TTATGAGCAA AGGAAATCTG CTAGACTATT TACGGAACGA GAGTAAACAT 
CAAATCAATG CTGTCGTTTT GATGCACATG GCCACACAGA TTGCTAGTGG AATGAGTTAT 
TTGGAGAGTA GAAATTTTAT ACACAGAGAT CTAGCAGCTC GAAACTGCCT AGTGGGTGAA 
AACCATCTGG TAAAGGTCGC AGACTTTGGC TTGGCCCGGT TGATGAGAGA TGATACGTAT 
ACGGCTCACG CTGGAGCAAA GTTTCCAATA AAATGGACTG CTCCAGAAGG ATTAGCTTAC 
AATAAATTCT CTACGAAGTC TGATGTGTGG GCATTTGGAA TTTTACTTTG GGAGATAGCA 
ACCTATGGCA TGTCCCCATA CCCTGGTGTT GATTTGACGG ATGTGTATCA CATGTTGGAG 
AAAGGCTACC GAATGGAATG TCCACCTGGA TGTCCACCGA AGGTATATGA ACTGATGCGT 
CAATGCTGGC AGTGGGCTGC TGCTGACCGG CCTACCTTCA AAGAGATTCA TCATTCTCTT 
GAAAATATGT TCCAAGAATC CAGTATTACC GAGGAAGTTG AAAAACAACT GCAAGGTGGC 
GGCGATATCC CTTTACTTTC ATATAAAAAA TCTCAAACTG GTAGTACTGG AAACATACAT 
GGGCTTGTTC TAGTCTCCGA GCCATTACCC TCTTCAGAAA CTACTAGCTC GGTAACAAAA 
CTGAGTACGT TTACGGGTGG TCTATCGAGT AAAAATAACA GCAATATCGT CCAAATGAGG 
CGTTCTACGA ATAAGAAAGG AAAACAAGCT CCGGCACCTC CAAAGAGAAC GAGCCTGCTG 
TCGTCGTGCA GTTCGTTTCG CGATTCTGCC TACCAAGAGC AAGATACCCA AAATACTGAA 
ACAAACACCA TGACTCTCGA CGATGCCACG GATCTAAATG GTATTGATAA GATTCTCGAA 
GGTATTGCGC GAGATCTCGC GACGATGGCT CAAGGAACGA TTGCTGCAGG AGGCTGCGAA 
GTCGAGGAGG ATGGAGAGGG TTCTCAAGGT ACGGCGGAAC CAAATTTTAT CCCTCAACCT 
TCGACGTCCC CGGAGCCTAT ACCTGGACTA GTAGCCTGTT CTCAGAAACA AATCAAACCA 
CGACCTTATC CATCAAAGGA ACCGTTACCA CAGAAGCTGG TACAAGTGGG AGCATTAGAA 
GTACAGAACG TAAAAAGAGC CATTAATCGT TATGGCACAC TACCAAAAGG TGCTAGGATC 
GGAGCATACC TAGAATCTTT GCGTCAAAGT GGTATGCCAT CGAATCAAGA ATCTACAACG 
GTGGTTTCTT CCATTGCACC TAGTTCAGTA GAACAACATG AGACTTCTAT GGACAATTCG 
CAACATAGAT CTCTCTCTCC TCGTCAAAGC AATCTACGAA ATCAACCGCA AATGACTCGC 
AGTAATTCCT CGAGCGGTGT TGTCAATACC TACCAACCTC CGAATTCCCC TCGTGGCCGA 
GTAGTTACTG TAAGAAAGAA CAATCAGTCT GATGGTGTTG GTCTGAGAAC ATTTCGAGTT 
TCAAGTAACT CGAACTTCAG AACTGCAAGT CCATCAAGAT CCGTTCAACC ATCTTTAGCG 
GATCTAGAGT TTCCGCCCCC ACCCGCAGAT TTGCCTCCTC CCCCTGACGA AACTTTCTCT 
GGTCAAGAAC AAGCTGAACT TCCGCCTCCC GTTTCTTGTA CCGAGCTATC TCAGATAAGA 
AATTCTCCTC TTTCTATAAG AAAAGCAAAG AGCACGGATT GGCGCACGAA AGAAGAAGAT 
AATGAACAGG AAGACAGGAA CGACGTTAGT AACATAGAGC CTTCCGTCAA AGAAGCTTGT 
TCGAGGTTTG GAGTTAATTT AAGGCGCAGA GAAACTCAAG ATAATTCTTG TAGAAACTCT 
GATAATAAAA GAACGGGATT TAAATCCAGA ATGGAGACAG TAGAGCCTGC TGCACCGCCA 
GAGGAAGCAC CACCTCCCCC ACCGCCACCT CCGCCGCCAC TTTCTACGAA TACACCCCCC 
GATAGCTTCG AACGTAAACC AGGCATGAAA GAAATGTTAG AATTAAAATT GATCAATGAA 
ATTAAACAGA GCGCGGAAAC AAAACATGGT ACGACTACGA AAAAGACTGG AGCGACAAGC 
AATACTCCAT CCGCACCTTT AGATCCAGCG TCGCAGCTAC TCTCGGAACT TTGTGCTAGT 
TTTAATATGG AGTCTGGACA GAGACACGCC CAAAACGAGT ACGCTGTATC AACTTTAAAA 
ACCAATGACG TAAATCAAGA ACAACAACAG CAACAAAGTC ATAACGCTCA TAAGGATTCG 
TCGGTGTCCT CTCCAGTGAC CGAGTCTCTC CTGTCCAGTG GAAATGTTGG ATTCAAGCTG 
AAAAGAGTTG ACAAGCGGAA TAATCCGCAA AAAGAAGAAA CATCCGATGG ACAAATAATT 
GATTTTAAAG CGAGACTTCG AAAAGTTGAG AACGCAGAAA AAGAAAAATC CTTGGAAGAG 
AAAAGTACCG TCACTGAACA GTCTTCAGAA TCAGAAGAGC AACAAGATGA CAAACGTAGA 
AGCACTGGTA GTATCAGTAG TTTAAAGAAG CTTTGGGAGA ACAAAGAATC TTGTGACAGT 
CAGCCTCATA GTCCAAAACT CAATGTCAGA GGAAGTGGTG GTAAACAAGA AATGATAGAC 
CAGACAGAAG ACTCACCGGA GGATCATAGT GGAGCTTCGA CACGTAGTCA GAGATTCGTG 
TTTGTAGTTC CGGTAGCAAA AGTGACGCTA GACTATGGCC ACCGCCCGAA CCTGAAAAAC 
CGGCAGTTCC AGCAAAGCCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012141832.1
CDS	279..4202
Translation	MGAQQTKERIVPAGSTARQTRKQPRNLKESRLVGSNIFTEHSEALLQSRPLPHIPALPDGDPPSGSAIQPISQQVNIQQHAGVSSTGLLEAANRWTSKENLLAQEEDDPQLFVALYDFQAGGENQLSLKKGEQVRILSYNKSGEWCEAHSSTGQVGWVPSNYVTPVNSLEKHSWYHGRISRNAAEYLLSSGINGSFLVRESESSPGQRSISLRYEGRVYHYRINEDSEGKMFVTTESKFNTLAELVHHHSMLADGLITQLLYPAPKHNKPTVFPLSPEPDEWEINRTDIVMRHKLGGGQYGDVYEAVWKRYNMTVAVKTLKEDTMALKDFLEEAAIMKEMKHRNLVQLLGVCTREPPFYIITEFMSKGNLLDYLRNESKHQINAVVLMHMATQIASGMSYLESRNFIHRDLAARNCLVGENHLVKVADFGLARLMRDDTYTAHAGAKFPIKWTAPEGLAYNKFSTKSDVWAFGILLWEIATYGMSPYPGVDLTDVYHMLEKGYRMECPPGCPPKVYELMRQCWQWAAADRPTFKEIHHSLENMFQESSITEEVEKQLQGGGDIPLLSYKKSQTGSTGNIHGLVLVSEPLPSSETTSSVTKLSTFTGGLSSKNNSNIVQMRRSTNKKGKQAPAPPKRTSLLSSCSSFRDSAYQEQDTQNTETNTMTLDDATDLNGIDKILEGIARDLATMAQGTIAAGGCEVEEDGEGSQGTAEPNFIPQPSTSPEPIPGLVACSQKQIKPRPYPSKEPLPQKLVQVGALEVQNVKRAINRYGTLPKGARIGAYLESLRQSGMPSNQESTTVVSSIAPSSVEQHETSMDNSQHRSLSPRQSNLRNQPQMTRSNSSSGVVNTYQPPNSPRGRVVTVRKNNQSDGVGLRTFRVSSNSNFRTASPSRSVQPSLADLEFPPPPADLPPPPDETFSGQEQAELPPPVSCTELSQIRNSPLSIRKAKSTDWRTKEEDNEQEDRNDVSNIEPSVKEACSRFGVNLRRRETQDNSCRNSDNKRTGFKSRMETVEPAAPPEEAPPPPPPPPPPLSTNTPPDSFERKPGMKEMLELKLINEIKQSAETKHGTTTKKTGATSNTPSAPLDPASQLLSELCASFNMESGQRHAQNEYAVSTLKTNDVNQEQQQQQSHNAHKDSSVSSPVTESLLSSGNVGFKLKRVDKRNNPQKEETSDGQIIDFKARLRKVENAEKEKSLEEKSTVTEQSSESEEQQDDKRRSTGSISSLKKLWENKESCDSQPHSPKLNVRGSGGKQEMIDQTEDSPEDHSGASTRSQRFVFVVPVAKVTLDYGHRPNLKNRQFQQSH

Target ORF information:

RefSeq Version	XM_012286442.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata tyrosine-protein kinase Abl (LOC100878636), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012286442.1

ORF Insert Sequence:

ATGGGTGCTC AACAGACTAA GGAAAGAATT GTTCCTGCCG GCTCTACTGC GCGACAGACT 
CGCAAACAGC CAAGGAACCT CAAGGAGTCT CGTCTTGTTG GTTCCAATAT ATTTACAGAA 
CACAGCGAAG CTCTCTTACA AAGCAGACCG CTACCTCACA TTCCGGCATT ACCAGATGGC 
GATCCTCCTA GTGGCTCTGC TATCCAACCA ATTTCACAAC AAGTGAATAT TCAACAACAT 
GCTGGTGTTT CATCTACAGG GCTTTTGGAA GCTGCAAACA GGTGGACAAG TAAAGAAAAC 
TTATTAGCAC AGGAGGAAGA TGATCCACAA CTATTTGTGG CTCTATATGA TTTTCAAGCA 
GGTGGAGAAA ACCAACTTAG TCTTAAAAAG GGAGAGCAAG TTCGTATTCT AAGTTATAAT 
AAAAGTGGAG AATGGTGTGA AGCTCATTCA AGTACTGGTC AAGTGGGGTG GGTTCCTTCA 
AATTATGTCA CCCCTGTAAA TTCTTTGGAA AAACATTCTT GGTATCATGG AAGGATATCC 
AGAAATGCTG CTGAATATCT CCTAAGTTCT GGTATAAATG GTAGCTTTCT GGTTCGTGAA 
TCAGAAAGCA GTCCAGGTCA ACGCAGTATT TCTTTGAGAT ATGAGGGTAG AGTCTATCAT 
TATAGGATTA ATGAGGATAG TGAAGGAAAG ATGTTTGTCA CAACAGAAAG CAAATTTAAT 
ACTCTGGCAG AATTGGTACA TCATCATTCT ATGCTTGCTG ATGGTTTAAT CACACAGCTA 
CTTTATCCTG CACCAAAGCA TAACAAACCA ACTGTTTTTC CACTTAGTCC AGAACCAGAT 
GAATGGGAGA TTAATAGGAC AGATATAGTT ATGAGGCATA AGTTAGGTGG AGGACAATAT 
GGAGATGTCT ATGAAGCAGT ATGGAAAAGA TATAACATGA CTGTGGCTGT AAAAACATTA 
AAAGAAGACA CAATGGCCCT AAAGGACTTT TTGGAGGAAG CTGCAATCAT GAAGGAGATG 
AAGCATAGGA ATTTAGTTCA GCTACTGGGT GTATGTACTC GAGAACCACC TTTCTACATC 
ATTACAGAGT TTATGAGCAA AGGAAATCTG CTAGACTATT TACGGAACGA GAGTAAACAT 
CAAATCAATG CTGTCGTTTT GATGCACATG GCCACACAGA TTGCTAGTGG AATGAGTTAT 
TTGGAGAGTA GAAATTTTAT ACACAGAGAT CTAGCAGCTC GAAACTGCCT AGTGGGTGAA 
AACCATCTGG TAAAGGTCGC AGACTTTGGC TTGGCCCGGT TGATGAGAGA TGATACGTAT 
ACGGCTCACG CTGGAGCAAA GTTTCCAATA AAATGGACTG CTCCAGAAGG ATTAGCTTAC 
AATAAATTCT CTACGAAGTC TGATGTGTGG GCATTTGGAA TTTTACTTTG GGAGATAGCA 
ACCTATGGCA TGTCCCCATA CCCTGGTGTT GATTTGACGG ATGTGTATCA CATGTTGGAG 
AAAGGCTACC GAATGGAATG TCCACCTGGA TGTCCACCGA AGGTATATGA ACTGATGCGT 
CAATGCTGGC AGTGGGCTGC TGCTGACCGG CCTACCTTCA AAGAGATTCA TCATTCTCTT 
GAAAATATGT TCCAAGAATC CAGTATTACC GAGGAAGTTG AAAAACAACT GCAAGGTGGC 
GGCGATATCC CTTTACTTTC ATATAAAAAA TCTCAAACTG GTAGTACTGG AAACATACAT 
GGGCTTGTTC TAGTCTCCGA GCCATTACCC TCTTCAGAAA CTACTAGCTC GGTAACAAAA 
CTGAGTACGT TTACGGGTGG TCTATCGAGT AAAAATAACA GCAATATCGT CCAAATGAGG 
CGTTCTACGA ATAAGAAAGG AAAACAAGCT CCGGCACCTC CAAAGAGAAC GAGCCTGCTG 
TCGTCGTGCA GTTCGTTTCG CGATTCTGCC TACCAAGAGC AAGATACCCA AAATACTGAA 
ACAAACACCA TGACTCTCGA CGATGCCACG GATCTAAATG GTATTGATAA GATTCTCGAA 
GGTATTGCGC GAGATCTCGC GACGATGGCT CAAGGAACGA TTGCTGCAGG AGGCTGCGAA 
GTCGAGGAGG ATGGAGAGGG TTCTCAAGGT ACGGCGGAAC CAAATTTTAT CCCTCAACCT 
TCGACGTCCC CGGAGCCTAT ACCTGGACTA GTAGCCTGTT CTCAGAAACA AATCAAACCA 
CGACCTTATC CATCAAAGGA ACCGTTACCA CAGAAGCTGG TACAAGTGGG AGCATTAGAA 
GTACAGAACG TAAAAAGAGC CATTAATCGT TATGGCACAC TACCAAAAGG TGCTAGGATC 
GGAGCATACC TAGAATCTTT GCGTCAAAGT GGTATGCCAT CGAATCAAGA ATCTACAACG 
GTGGTTTCTT CCATTGCACC TAGTTCAGTA GAACAACATG AGACTTCTAT GGACAATTCG 
CAACATAGAT CTCTCTCTCC TCGTCAAAGC AATCTACGAA ATCAACCGCA AATGACTCGC 
AGTAATTCCT CGAGCGGTGT TGTCAATACC TACCAACCTC CGAATTCCCC TCGTGGCCGA 
GTAGTTACTG TAAGAAAGAA CAATCAGTCT GATGGTGTTG GTCTGAGAAC ATTTCGAGTT 
TCAAGTAACT CGAACTTCAG AACTGCAAGT CCATCAAGAT CCGTTCAACC ATCTTTAGCG 
GATCTAGAGT TTCCGCCCCC ACCCGCAGAT TTGCCTCCTC CCCCTGACGA AACTTTCTCT 
GGTCAAGAAC AAGCTGAACT TCCGCCTCCC GTTTCTTGTA CCGAGCTATC TCAGATAAGA 
AATTCTCCTC TTTCTATAAG AAAAGCAAAG AGCACGGATT GGCGCACGAA AGAAGAAGAT 
AATGAACAGG AAGACAGGAA CGACGTTAGT AACATAGAGC CTTCCGTCAA AGAAGCTTGT 
TCGAGGTTTG GAGTTAATTT AAGGCGCAGA GAAACTCAAG ATAATTCTTG TAGAAACTCT 
GATAATAAAA GAACGGGATT TAAATCCAGA ATGGAGACAG TAGAGCCTGC TGCACCGCCA 
GAGGAAGCAC CACCTCCCCC ACCGCCACCT CCGCCGCCAC TTTCTACGAA TACACCCCCC 
GATAGCTTCG AACGTAAACC AGGCATGAAA GAAATGTTAG AATTAAAATT GATCAATGAA 
ATTAAACAGA GCGCGGAAAC AAAACATGGT ACGACTACGA AAAAGACTGG AGCGACAAGC 
AATACTCCAT CCGCACCTTT AGATCCAGCG TCGCAGCTAC TCTCGGAACT TTGTGCTAGT 
TTTAATATGG AGTCTGGACA GAGACACGCC CAAAACGAGT ACGCTGTATC AACTTTAAAA 
ACCAATGACG TAAATCAAGA ACAACAACAG CAACAAAGTC ATAACGCTCA TAAGGATTCG 
TCGGTGTCCT CTCCAGTGAC CGAGTCTCTC CTGTCCAGTG GAAATGTTGG ATTCAAGCTG 
AAAAGAGTTG ACAAGCGGAA TAATCCGCAA AAAGAAGAAA CATCCGATGG ACAAATAATT 
GATTTTAAAG CGAGACTTCG AAAAGTTGAG AACGCAGAAA AAGAAAAATC CTTGGAAGAG 
AAAAGTACCG TCACTGAACA GTCTTCAGAA TCAGAAGAGC AACAAGATGA CAAACGTAGA 
AGCACTGGTA GTATCAGTAG TTTAAAGAAG CTTTGGGAGA ACAAAGAATC TTGTGACAGT 
CAGCCTCATA GTCCAAAACT CAATGTCAGA GGAAGTGGTG GTAAACAAGA AATGATAGAC 
CAGACAGAAG ACTCACCGGA GGATCATAGT GGAGCTTCGA CACGTAGTCA GAGATTCGTG 
TTTGTAGTTC CGGTAGCAAA AGTGACGCTA GACTATGGCC ACCGCCCGAA CCTGAAAAAC 
CGGCAGTTCC AGCAAAGCCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

LOC100878636 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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