LOC100877885 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100877885
Entrez Gene ID	100877885
Full Name	protein-L-isoaspartate(D-aspartate) O-methyltransferase-like
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012286707.1	XP_012142097.1	protein-L-isoaspartate(D-aspartate) O-methyltransferase-like

Pathways from BioSystems
Gene Ontology

The following LOC100877885 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100877885 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe105891	XM_012286707.1 Latest version!	Megachile rotundata protein-L-isoaspartate(D-aspartate) O-methyltransferase-like (LOC100877885), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100877885 ( XM_012286707.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012142097.1 Megachile rotundata protein-L-isoaspartate(D-aspartate) O-methyltransferase-like (LOC100877885), mRNA.

Price & Availability↑

CloneID	OMe105891
Clone ID Related Accession (Same CDS sequence)	XM_012286707.1
Accession Version	XM_012286707.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	protein-L-isoaspartate(D-aspartate) O-methyltransferase-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797182.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTTT TACTGACATA TGTAGATAAA GTAGCGATTG AATATAACGA TGTAATTAGT 
TTATTATTTT CCGTATTTGC ATATGAAATG GCCTGGTCTG GAAGATTTCA TGGGAAAAGT 
AACCATGATC TTATACAAAA TCTTAAAAGA TCAAGAGTAA TTAAATCTGA ACGAGTTTAT 
GAAGCAATGT CTTCTGTAGA CAGAGGAAAT TATACTCATC CCAGCTATGC ATATGTAGAT 
TCTCCGCAAT CAATTGGTTA TGGTGCTACT ATAAGTGCTC CTCATATGCA TGCATATGCT 
TTGGAAATAC TTGAAGATAA ATTACGTGAT GGTGCAAGAG CATTAGATGT TGGTTCTGGA 
TCTGGATATT TGACAGCATG CATGGCAATG ATGTTAGGTC CAAATGGGTT GGCTATTGGT 
ATTGATCATA TTCCTGAACT TAAATCATTT GCTATTTCCA ACATTCAAAG GGATAATCCA 
GAGCTTCTTA AAAGTGGTCG TGTTGAATTA GTTGTTGGAG ATGGAAGATT AGGATATCCT 
GAGAAGGCAC CATACAATGC TATTCATGTA GGAGCAGCTG CTAAAGAAAT GCCACAAGCT 
TTGATTGATC AACTAGCTCC TGGAGGACGT TTAGTATTAC CAATGGGTCC AGAAAATTCA 
GATCAAACAT TAGTACAAGT TGATAAGACA ATGGATGGAA AAATTCTGAA AAAATCTCTT 
ACAAGTGTTG TTTTTGTCCC ATTAACTAGT AAAAATAAGC AACATCCATC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012142097.1
CDS	79..852
Translation	MEVLLTYVDKVAIEYNDVISLLFSVFAYEMAWSGRFHGKSNHDLIQNLKRSRVIKSERVYEAMSSVDRGNYTHPSYAYVDSPQSIGYGATISAPHMHAYALEILEDKLRDGARALDVGSGSGYLTACMAMMLGPNGLAIGIDHIPELKSFAISNIQRDNPELLKSGRVELVVGDGRLGYPEKAPYNAIHVGAAAKEMPQALIDQLAPGGRLVLPMGPENSDQTLVQVDKTMDGKILKKSLTSVVFVPLTSKNKQHPS

Target ORF information:

RefSeq Version	XM_012286707.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata protein-L-isoaspartate(D-aspartate) O-methyltransferase-like (LOC100877885), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012286707.1

ORF Insert Sequence:

ATGGAAGTTT TACTGACATA TGTAGATAAA GTAGCGATTG AATATAACGA TGTAATTAGT 
TTATTATTTT CCGTATTTGC ATATGAAATG GCCTGGTCTG GAAGATTTCA TGGGAAAAGT 
AACCATGATC TTATACAAAA TCTTAAAAGA TCAAGAGTAA TTAAATCTGA ACGAGTTTAT 
GAAGCAATGT CTTCTGTAGA CAGAGGAAAT TATACTCATC CCAGCTATGC ATATGTAGAT 
TCTCCGCAAT CAATTGGTTA TGGTGCTACT ATAAGTGCTC CTCATATGCA TGCATATGCT 
TTGGAAATAC TTGAAGATAA ATTACGTGAT GGTGCAAGAG CATTAGATGT TGGTTCTGGA 
TCTGGATATT TGACAGCATG CATGGCAATG ATGTTAGGTC CAAATGGGTT GGCTATTGGT 
ATTGATCATA TTCCTGAACT TAAATCATTT GCTATTTCCA ACATTCAAAG GGATAATCCA 
GAGCTTCTTA AAAGTGGTCG TGTTGAATTA GTTGTTGGAG ATGGAAGATT AGGATATCCT 
GAGAAGGCAC CATACAATGC TATTCATGTA GGAGCAGCTG CTAAAGAAAT GCCACAAGCT 
TTGATTGATC AACTAGCTCC TGGAGGACGT TTAGTATTAC CAATGGGTCC AGAAAATTCA 
GATCAAACAT TAGTACAAGT TGATAAGACA ATGGATGGAA AAATTCTGAA AAAATCTCTT 
ACAAGTGTTG TTTTTGTCCC ATTAACTAGT AAAAATAAGC AACATCCATC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.