LOC100877494 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100877494
Entrez Gene ID	100877494
Full Name	uncharacterized LOC100877494
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012297758.1	XP_012153148.1	uncharacterized protein LOC100877494

Pathways from BioSystems
Gene Ontology

The following LOC100877494 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100877494 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe115556	XM_012297758.1 Latest version!	Megachile rotundata uncharacterized LOC100877494 (LOC100877494), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100877494 ( XM_012297758.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012153148.1 Megachile rotundata uncharacterized LOC100877494 (LOC100877494), mRNA.

Price & Availability↑

CloneID	OMe115556
Clone ID Related Accession (Same CDS sequence)	XM_012297758.1
Accession Version	XM_012297758.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	uncharacterized protein LOC100877494
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003799051.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAC TCGGAAGATT CGTAGCTTTC TGGACGATTC TATTTTTCTG CGATTATTTA 
CGGTCGATCG ATGGAAAGTT CGTTCCTGAA CATTTCGGTG AGATCACTTT ATTCTACAGG 
AGATTATTGC TGTCGACGCG ACGGTCAAGT TACTTGGAAT CGTTGATTTT AGAACGAAAG 
TTATTATCGC TTTCGAAGGT AATCGATTAC ATTGGTCGTA GACCCCATCA AATGAACGCC 
GATGTCACAC TGTCTATCAC CATCGTTCAA GTTAACATAG CCAGCACGCT TCTCCACGAA 
CACGCGCTAT ACCTTCGAAG CGAATATCGC GAGATTCTCA CGGGAATTTT ACAAGTTTCC 
GATTCGATTC GACGATACCT GCTCGGTATT ATAATTCCAG GAAACGAGGA CGTTCGACTA 
CTGCACGAGA CGTTGAATAA TCCAGTTTTA TGGATGCGAG CAATACCCTG GCGAAACGGT 
GCCCTAGAAA CATGGAAAGC CAATTGTTCG TTAACTTGGC GAAATATACG AGAGGTGATA 
ATGCAAGGAA CACCCAAAGA AGAAGAAAGC GACCGATGCC TCGCTCAGAT TATTCGGAAT 
AAATCGTATT TCGGTTGCTC GATTCCCGAG CTATGCGTTG AAATATTGAT GGAAGCTGGT 
CTTACCAGAG GATATCCTCT CACGCATAGA TTATTGATCG TTCAAGTTGC CAAAGCGATG 
AAATGCGATC AACCTCTGCC CGTTTCATCC GACGAATTGA TCCTGTATTA TTGTTCGAAG 
ATTCTTCAGG ATCTGATCGA CATCGAGAGA GCCGGATTTC CCTCTCATAC ACCGATGGAT 
TTGATGATGG AAGAAGTTCT TCTCTGCGGT ATGGAAGGGT TCCTCGAGTT CACCGACGAT 
CACTACCAGC GTTTAATAAC TAGCTGGTCT CATCCTAGTG GCTGCTTCAG CTCCTTCGGA 
AACGGATTTT CCAACGACCG GATCCACGTG GCTCGAAGAT CTTCGGCCGA AACCGATTTC 
GGCTGCGACA ACCACGCGAC CGGTCTCGCG GCGGCAGCCC TATCCTTATT TATTCGTAAA 
AACGTGGAGA TCGCGTTCGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012153148.1
CDS	23..1126
Translation	MERLGRFVAFWTILFFCDYLRSIDGKFVPEHFGEITLFYRRLLLSTRRSSYLESLILERKLLSLSKVIDYIGRRPHQMNADVTLSITIVQVNIASTLLHEHALYLRSEYREILTGILQVSDSIRRYLLGIIIPGNEDVRLLHETLNNPVLWMRAIPWRNGALETWKANCSLTWRNIREVIMQGTPKEEESDRCLAQIIRNKSYFGCSIPELCVEILMEAGLTRGYPLTHRLLIVQVAKAMKCDQPLPVSSDELILYYCSKILQDLIDIERAGFPSHTPMDLMMEEVLLCGMEGFLEFTDDHYQRLITSWSHPSGCFSSFGNGFSNDRIHVARRSSAETDFGCDNHATGLAAAALSLFIRKNVEIAFD

Target ORF information:

RefSeq Version	XM_012297758.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata uncharacterized LOC100877494 (LOC100877494), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012297758.1

ORF Insert Sequence:

ATGGAGAGAC TCGGAAGATT CGTAGCTTTC TGGACGATTC TATTTTTCTG CGATTATTTA 
CGGTCGATCG ATGGAAAGTT CGTTCCTGAA CATTTCGGTG AGATCACTTT ATTCTACAGG 
AGATTATTGC TGTCGACGCG ACGGTCAAGT TACTTGGAAT CGTTGATTTT AGAACGAAAG 
TTATTATCGC TTTCGAAGGT AATCGATTAC ATTGGTCGTA GACCCCATCA AATGAACGCC 
GATGTCACAC TGTCTATCAC CATCGTTCAA GTTAACATAG CCAGCACGCT TCTCCACGAA 
CACGCGCTAT ACCTTCGAAG CGAATATCGC GAGATTCTCA CGGGAATTTT ACAAGTTTCC 
GATTCGATTC GACGATACCT GCTCGGTATT ATAATTCCAG GAAACGAGGA CGTTCGACTA 
CTGCACGAGA CGTTGAATAA TCCAGTTTTA TGGATGCGAG CAATACCCTG GCGAAACGGT 
GCCCTAGAAA CATGGAAAGC CAATTGTTCG TTAACTTGGC GAAATATACG AGAGGTGATA 
ATGCAAGGAA CACCCAAAGA AGAAGAAAGC GACCGATGCC TCGCTCAGAT TATTCGGAAT 
AAATCGTATT TCGGTTGCTC GATTCCCGAG CTATGCGTTG AAATATTGAT GGAAGCTGGT 
CTTACCAGAG GATATCCTCT CACGCATAGA TTATTGATCG TTCAAGTTGC CAAAGCGATG 
AAATGCGATC AACCTCTGCC CGTTTCATCC GACGAATTGA TCCTGTATTA TTGTTCGAAG 
ATTCTTCAGG ATCTGATCGA CATCGAGAGA GCCGGATTTC CCTCTCATAC ACCGATGGAT 
TTGATGATGG AAGAAGTTCT TCTCTGCGGT ATGGAAGGGT TCCTCGAGTT CACCGACGAT 
CACTACCAGC GTTTAATAAC TAGCTGGTCT CATCCTAGTG GCTGCTTCAG CTCCTTCGGA 
AACGGATTTT CCAACGACCG GATCCACGTG GCTCGAAGAT CTTCGGCCGA AACCGATTTC 
GGCTGCGACA ACCACGCGAC CGGTCTCGCG GCGGCAGCCC TATCCTTATT TATTCGTAAA 
AACGTGGAGA TCGCGTTCGA TTAA