LOC100877353 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100877353
Entrez Gene ID	100877353
Full Name	uncharacterized LOC100877353
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012279512.1	XP_012134902.1	uncharacterized protein LOC100877353
XM_003700637.2	XP_003700685.1	uncharacterized protein LOC100877353

Pathways from BioSystems
Gene Ontology

The following LOC100877353 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100877353 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe99548	XM_003700637.2 Latest version!	Megachile rotundata uncharacterized LOC100877353 (LOC100877353), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OMe99548	XM_012279512.1 Latest version!	Megachile rotundata uncharacterized LOC100877353 (LOC100877353), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100877353 ( XM_012279512.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012134902.1 Megachile rotundata uncharacterized LOC100877353 (LOC100877353), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMe99548
Clone ID Related Accession (Same CDS sequence)	XM_012279512.1 , XM_003700637.2
Accession Version	XM_012279512.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	uncharacterized protein LOC100877353
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797099.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGACCA TGGGTGTCAC CGTCTTCTGC AACAGGGTGC AGATCAACGG CAACGATCAG 
GAGCAGCTGA TGCTGCTGCG GACGTTGCAG GACAACGAGA GCAACATAAT CGGCAATCAG 
CCTCATCTGA TAGTCGCAAA GAACAACAAC AACAACGGTA ATACGAACCC GTCGATCGTT 
TTACCGCCCT ACGATCCATC CAGACTGAAG AAACACGGGA AGAACGGTCA GCCGCCGCCA 
GTCGCCGTTG CCCGAAGGAA TGCCCGCGAG CGTAATCGCG TCAAGCAGGT GAACAATGGG 
TTCGCGACTT TGAGACAACA CATCCCGAGC CACATCGCAG CTGGCTACGG TGACAGAGGC 
AAGAAACTGT CGAAAGTGGA AACCCTGCGA ATGGCTGTGG AGTACATCAG AGGTCTTCAG 
AGGCTGCTGG CCGAAGCTGA CGGGATCGAA TACGACGCCA ACGCCGGTGC CGGGCAATGT 
ATGCCCTCAC CTACCAGCAG TGTCGTTTCG TCGAGTCACA ACGGATCCGG GGAGCGACTT 
ATCCTCGAGG ACGACGTTCT CGGCGCTGAA GAGGAGGACG GCGAACAACT GGAGGAGGAC 
GATGATAATG GGAAGCAGAA AACTACGTCT AGATTGTCGC CGAATCGAAC TACCGTCGCA 
TCGCCACACG ATTATTACGC GTCCTCGGTG GGCGACGAAG AGAATCTCGA ACCGAGAACC 
GTATCGAACC CGCAGAATTT TTCACGACTG TCCCCGAACT CGGTGGCATC ACCACCGTCG 
GAGTATTACG GGCAAAACGA GGAGAACCTG GAACCTCAGA TCCTCTCGCC ATATAGTGGC 
GCCGGGGACA GTGAAGAGGG TGCAGCCACT GTTTATGCGG CGAGCCCGGA AACCTTTTCT 
GGGGCTCTAT ATTATAAGCA GGAGATCACT GAATCCGGTG AATTCATGGA CGTCGTCAGC 
TGGTGGGAAC AAGAGCAGGG CAGGCTGGTT CATCAGCAAC ACACCCAACG ACTTACGCAC 
GTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012134902.1
CDS	1096..2121
Translation	MTTMGVTVFCNRVQINGNDQEQLMLLRTLQDNESNIIGNQPHLIVAKNNNNNGNTNPSIVLPPYDPSRLKKHGKNGQPPPVAVARRNARERNRVKQVNNGFATLRQHIPSHIAAGYGDRGKKLSKVETLRMAVEYIRGLQRLLAEADGIEYDANAGAGQCMPSPTSSVVSSSHNGSGERLILEDDVLGAEEEDGEQLEEDDDNGKQKTTSRLSPNRTTVASPHDYYASSVGDEENLEPRTVSNPQNFSRLSPNSVASPPSEYYGQNEENLEPQILSPYSGAGDSEEGAATVYAASPETFSGALYYKQEITESGEFMDVVSWWEQEQGRLVHQQHTQRLTHV

Target ORF information:

RefSeq Version	XM_012279512.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata uncharacterized LOC100877353 (LOC100877353), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012279512.1

ORF Insert Sequence:

ATGACGACCA TGGGTGTCAC CGTCTTCTGC AACAGGGTGC AGATCAACGG CAACGATCAG 
GAGCAGCTGA TGCTGCTGCG GACGTTGCAG GACAACGAGA GCAACATAAT CGGCAATCAG 
CCTCATCTGA TAGTCGCAAA GAACAACAAC AACAACGGTA ATACGAACCC GTCGATCGTT 
TTACCGCCCT ACGATCCATC CAGACTGAAG AAACACGGGA AGAACGGTCA GCCGCCGCCA 
GTCGCCGTTG CCCGAAGGAA TGCCCGCGAG CGTAATCGCG TCAAGCAGGT GAACAATGGG 
TTCGCGACTT TGAGACAACA CATCCCGAGC CACATCGCAG CTGGCTACGG TGACAGAGGC 
AAGAAACTGT CGAAAGTGGA AACCCTGCGA ATGGCTGTGG AGTACATCAG AGGTCTTCAG 
AGGCTGCTGG CCGAAGCTGA CGGGATCGAA TACGACGCCA ACGCCGGTGC CGGGCAATGT 
ATGCCCTCAC CTACCAGCAG TGTCGTTTCG TCGAGTCACA ACGGATCCGG GGAGCGACTT 
ATCCTCGAGG ACGACGTTCT CGGCGCTGAA GAGGAGGACG GCGAACAACT GGAGGAGGAC 
GATGATAATG GGAAGCAGAA AACTACGTCT AGATTGTCGC CGAATCGAAC TACCGTCGCA 
TCGCCACACG ATTATTACGC GTCCTCGGTG GGCGACGAAG AGAATCTCGA ACCGAGAACC 
GTATCGAACC CGCAGAATTT TTCACGACTG TCCCCGAACT CGGTGGCATC ACCACCGTCG 
GAGTATTACG GGCAAAACGA GGAGAACCTG GAACCTCAGA TCCTCTCGCC ATATAGTGGC 
GCCGGGGACA GTGAAGAGGG TGCAGCCACT GTTTATGCGG CGAGCCCGGA AACCTTTTCT 
GGGGCTCTAT ATTATAAGCA GGAGATCACT GAATCCGGTG AATTCATGGA CGTCGTCAGC 
TGGTGGGAAC AAGAGCAGGG CAGGCTGGTT CATCAGCAAC ACACCCAACG ACTTACGCAC 
GTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100877353 ( XM_003700637.2 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_003700685.1 Megachile rotundata uncharacterized LOC100877353 (LOC100877353), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMe99548
Clone ID Related Accession (Same CDS sequence)	XM_012279512.1 , XM_003700637.2
Accession Version	XM_003700637.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	uncharacterized protein LOC100877353
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797099.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2015 this sequence version replaced XM_003700637.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGACCA TGGGTGTCAC CGTCTTCTGC AACAGGGTGC AGATCAACGG CAACGATCAG 
GAGCAGCTGA TGCTGCTGCG GACGTTGCAG GACAACGAGA GCAACATAAT CGGCAATCAG 
CCTCATCTGA TAGTCGCAAA GAACAACAAC AACAACGGTA ATACGAACCC GTCGATCGTT 
TTACCGCCCT ACGATCCATC CAGACTGAAG AAACACGGGA AGAACGGTCA GCCGCCGCCA 
GTCGCCGTTG CCCGAAGGAA TGCCCGCGAG CGTAATCGCG TCAAGCAGGT GAACAATGGG 
TTCGCGACTT TGAGACAACA CATCCCGAGC CACATCGCAG CTGGCTACGG TGACAGAGGC 
AAGAAACTGT CGAAAGTGGA AACCCTGCGA ATGGCTGTGG AGTACATCAG AGGTCTTCAG 
AGGCTGCTGG CCGAAGCTGA CGGGATCGAA TACGACGCCA ACGCCGGTGC CGGGCAATGT 
ATGCCCTCAC CTACCAGCAG TGTCGTTTCG TCGAGTCACA ACGGATCCGG GGAGCGACTT 
ATCCTCGAGG ACGACGTTCT CGGCGCTGAA GAGGAGGACG GCGAACAACT GGAGGAGGAC 
GATGATAATG GGAAGCAGAA AACTACGTCT AGATTGTCGC CGAATCGAAC TACCGTCGCA 
TCGCCACACG ATTATTACGC GTCCTCGGTG GGCGACGAAG AGAATCTCGA ACCGAGAACC 
GTATCGAACC CGCAGAATTT TTCACGACTG TCCCCGAACT CGGTGGCATC ACCACCGTCG 
GAGTATTACG GGCAAAACGA GGAGAACCTG GAACCTCAGA TCCTCTCGCC ATATAGTGGC 
GCCGGGGACA GTGAAGAGGG TGCAGCCACT GTTTATGCGG CGAGCCCGGA AACCTTTTCT 
GGGGCTCTAT ATTATAAGCA GGAGATCACT GAATCCGGTG AATTCATGGA CGTCGTCAGC 
TGGTGGGAAC AAGAGCAGGG CAGGCTGGTT CATCAGCAAC ACACCCAACG ACTTACGCAC 
GTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003700685.1
CDS	1673..2698
Translation	MTTMGVTVFCNRVQINGNDQEQLMLLRTLQDNESNIIGNQPHLIVAKNNNNNGNTNPSIVLPPYDPSRLKKHGKNGQPPPVAVARRNARERNRVKQVNNGFATLRQHIPSHIAAGYGDRGKKLSKVETLRMAVEYIRGLQRLLAEADGIEYDANAGAGQCMPSPTSSVVSSSHNGSGERLILEDDVLGAEEEDGEQLEEDDDNGKQKTTSRLSPNRTTVASPHDYYASSVGDEENLEPRTVSNPQNFSRLSPNSVASPPSEYYGQNEENLEPQILSPYSGAGDSEEGAATVYAASPETFSGALYYKQEITESGEFMDVVSWWEQEQGRLVHQQHTQRLTHV

Target ORF information:

RefSeq Version	XM_003700637.2
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata uncharacterized LOC100877353 (LOC100877353), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003700637.2

ORF Insert Sequence:

ATGACGACCA TGGGTGTCAC CGTCTTCTGC AACAGGGTGC AGATCAACGG CAACGATCAG 
GAGCAGCTGA TGCTGCTGCG GACGTTGCAG GACAACGAGA GCAACATAAT CGGCAATCAG 
CCTCATCTGA TAGTCGCAAA GAACAACAAC AACAACGGTA ATACGAACCC GTCGATCGTT 
TTACCGCCCT ACGATCCATC CAGACTGAAG AAACACGGGA AGAACGGTCA GCCGCCGCCA 
GTCGCCGTTG CCCGAAGGAA TGCCCGCGAG CGTAATCGCG TCAAGCAGGT GAACAATGGG 
TTCGCGACTT TGAGACAACA CATCCCGAGC CACATCGCAG CTGGCTACGG TGACAGAGGC 
AAGAAACTGT CGAAAGTGGA AACCCTGCGA ATGGCTGTGG AGTACATCAG AGGTCTTCAG 
AGGCTGCTGG CCGAAGCTGA CGGGATCGAA TACGACGCCA ACGCCGGTGC CGGGCAATGT 
ATGCCCTCAC CTACCAGCAG TGTCGTTTCG TCGAGTCACA ACGGATCCGG GGAGCGACTT 
ATCCTCGAGG ACGACGTTCT CGGCGCTGAA GAGGAGGACG GCGAACAACT GGAGGAGGAC 
GATGATAATG GGAAGCAGAA AACTACGTCT AGATTGTCGC CGAATCGAAC TACCGTCGCA 
TCGCCACACG ATTATTACGC GTCCTCGGTG GGCGACGAAG AGAATCTCGA ACCGAGAACC 
GTATCGAACC CGCAGAATTT TTCACGACTG TCCCCGAACT CGGTGGCATC ACCACCGTCG 
GAGTATTACG GGCAAAACGA GGAGAACCTG GAACCTCAGA TCCTCTCGCC ATATAGTGGC 
GCCGGGGACA GTGAAGAGGG TGCAGCCACT GTTTATGCGG CGAGCCCGGA AACCTTTTCT 
GGGGCTCTAT ATTATAAGCA GGAGATCACT GAATCCGGTG AATTCATGGA CGTCGTCAGC 
TGGTGGGAAC AAGAGCAGGG CAGGCTGGTT CATCAGCAAC ACACCCAACG ACTTACGCAC 
GTGTAA