LOC100877177 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100877177
Entrez Gene ID	100877177
Full Name	probable multidrug resistance-associated protein lethal(2)03659
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003705819.2	XP_003705867.1	probable multidrug resistance-associated protein lethal(2)03659 isoform X1
XM_012291340.1	XP_012146730.1	probable multidrug resistance-associated protein lethal(2)03659 isoform X1
XM_012291341.1	XP_012146731.1	probable multidrug resistance-associated protein lethal(2)03659 isoform X3
XM_012291338.1	XP_012146728.1	probable multidrug resistance-associated protein lethal(2)03659 isoform X1
XM_012291339.1	XP_012146729.1	probable multidrug resistance-associated protein lethal(2)03659 isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC100877177 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100877177 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMe109917	XM_012291340.1 Latest version!	Megachile rotundata probable multidrug resistance-associated protein lethal(2)03659 (LOC100877177), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OMe109917	XM_012291338.1 Latest version!	Megachile rotundata probable multidrug resistance-associated protein lethal(2)03659 (LOC100877177), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OMe109917	XM_012291339.1 Latest version!	Megachile rotundata probable multidrug resistance-associated protein lethal(2)03659 (LOC100877177), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OMe109917	XM_003705819.2 Latest version!	Megachile rotundata probable multidrug resistance-associated protein lethal(2)03659 (LOC100877177), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OMe109918	XM_012291341.1 Latest version!	Megachile rotundata probable multidrug resistance-associated protein lethal(2)03659 (LOC100877177), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100877177 ( XM_003705819.2 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_003705867.1 Megachile rotundata probable multidrug resistance-associated protein lethal(2)03659 (LOC100877177), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMe109917
Clone ID Related Accession (Same CDS sequence)	XM_003705819.2 , XM_012291340.1 , XM_012291338.1 , XM_012291339.1
Accession Version	XM_003705819.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	probable multidrug resistance-associated protein lethal(2)03659 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797352.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2015 this sequence version replaced XM_003705819.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAA GTGAAAGGAA CCTATTGAAG AATCCGCGTC AAAATGCCAA TCTATTATCT 
ATTCTTACGT TCTGGTGGAT CCTCAAGTTG TTCATTGTCG GTTACAAAAA AGAATTGGAA 
GAAGAGGATT TGTATTGTCC ATTGAGAGAG GACAGGAGCA GTTACTTAGG CGAACGTATT 
GTAAAATATT GGGAGATTGA AGTGAAACGA CACGAGAAGA AGAAGGATAG CCCTAAACCA 
AGTTTATTTA GGGTATTGTA CAAATGCTTC GGTAAAATTG TCATGAACAC TGGACTAGCA 
CTGTTTGTTC TAGAATTCGG CATACGGATC ATACATCCTT TCCTTCTCGC TAGATTACTA 
CGTTACTTCG CTGGGAATAG AAAGGATTGG ACCAGTGATG TACATTACTA TGCAGCAGCA 
TTTTGTTTAC TACCTTTGCT AGATGTTCTG ATTATGCATT GGTCCTTACA AACCTTGACG 
CATGTCGGAA TGAAAATTAG AGTGGCTTGT TGTACCTTGA TTTATAGGAA GATATTGAGA 
CTATCCAATT CTGTCCTTGA AAATGAAACC TCTGCTGGGC AGATGGTTAA TTTCTTGAGT 
AACGACGTTA ATCGTCTCGA TTACTTCGTC TTTAGTATTC ATTATTTATG GATTGGACCT 
CTACAAGTAT TTCTAATAGC CTATTTAATA TATCGTGAGA TAGGCCTGGG AGCTATTACT 
GGGATGATGA CATTTTTATT ATGCATACCA TTACAGTTAT ATTTTGGAAA GAGAGTGTCA 
CGTTTGACTC TTGTATCGGC CCAGAAGACT GACAATAGAT TAAGTTTGAT GAACCAAATT 
ATAGCTGGTG TAGAATTAAT CAAGATGTAC GTTTGGGAGG TACCGTACTC TCTTCTCGTT 
GAAAAAGCTA GACGAAAAGA AGTGGATGTG ATCAAGAAAT ATTCGATTGT CGAGCAAATA 
GGTTTAACGT GTGATATCTA TGTTCCTCGA GTCAGCTTAT TTATTACGAT ATTAACCTAC 
GTTTTAACTG GCAACACCAT AGATGCCGAG AAAGTATTTA TGACTACTGC GTTTTACGCC 
ATTTTACAAA GCTCTATGAC CGTCGGTTTC ACTCTAAGTG TCCATCTGCT AGCTGAAGCC 
ATGGTGTCCG TCAGGCGTCT CGAGAAGTTC ATGATGCACC CCGAAATCAT TCCTCCGCCG 
AAGACGCAGA ATCAAGTAGC CACCCAATCA CTGCCCGTAT ACCTGAAAAA CGTGTCCGCC 
AGGTGGGACG AGTCCAGAGA CTGCACTTTA CAAAACGTAG ATTTATCGAT ACAAGCCGGA 
AGCTTCGTAG CGGTGATCGG ACAAATCGGT TCTGGAAAGA GTAGCCTCCT AAAAGCGATA 
CTCAGTGAGT TACCATTACA AGATGGCATC CTAGAAACCA GCGGTAAAAT AAGTTTCGCC 
GATCAACGAC CTTGGATATT TGCCTCCTCC ATCCGACAGA ACATACTGTT TGGTCAGCCT 
TACAACGAAA AGCGTTACAA CGAGGTGATT AAAGTCTGTC AACTAAAACG CGACATAGAT 
TCGTTTACGC ACAAAGACAG CACGATGGTC GGCGAAAGAG GGATCAATTT AAGTGGCGGT 
CAACGAGCTA GAATTAATTT GGCCCGTGCC ATCTACGCCG AGGCCGACAT ATATCTGTTG 
GATGATCCTC TATCGGCGGT CGACGGACAC GTGGGTAGCC ACATCGTGGA CGAGTGTATC 
TGCGGTTTCC TCAAGGGCAA GACTCGAATT CTAGTCACCC ATCAGATCCA GTACTTGAAA 
CCCGCCGATC AGATCGTCGT GATCAACGGC GGTGCCGTGC AAGCGAAGGG CAGCTTCGAG 
GAGCTTCAGA GGATGAGTCT GGACTTCATG AAGATCTTCA AAGAGGTGGA AGAGAGCAAG 
ACCAAAGAAC CCGAAACCGA GGAGAAGCAA ACGATCGAGG AGACGAAGAA AGAGGAAGAA 
CCGGAAGTAG GCAGTGAACC TGTCGAGGTG GCAGAAACGA GAACCGTAGG GAAAATTTCC 
GCCGCCGTTT TCTTAGCCTA CTGGAAAGCA AGTAAAAACC CGTGTCTGCT CGCTTTCATG 
GTGGTTCTGT TTATTTTGAG TCAAGTAATG GCGAGCGGCG CTGACTACTT GCTCGCGTTC 
TGGGTGAACA CCGAGGTAGC CTCGTGGGTT AATATCGGAA ATGGTACGAT GGCTTTTCAA 
TGGAATACTT TACTATCTCG CGATGGAATT ATTTATATTT ACAGCGCCTT AACGTTCGGC 
TTCGTCTGCG TCTATATCGT CGAAGCGTTC ACGTACTATG GGGTCTGCAT GAGAGCCTCG 
AAAAATTTGC ACGCGGCGAT GTTTCGTAGT ATTGTTCGGG CTACCATGTA CTTTTTCAAC 
ACTAATCCTG CTGGTCGTAT ATTAAACAGG TTTTCCAAAG ACATTGGTGC TATCGATAAG 
AAGCTTCCTT TCACGACGTT CGATGTCACA ATTATGTTCT TAACGTTTAT CGGAACTGTA 
GTAATTGTGG GTACTGTCAG CGCGTGGTTA TTGATACCGA CATTTGTCGC TCTACTCATT 
TTCTACTACA TGCGAGTTAT TTATATTGCA ACTAGTCGTT CTGTTAAACG TATGGAGGGT 
ACAACACGAT CGCCAGTGTT TGATCACGTC GGTGCAACTC TGCAGGGTCT CACGACAATC 
AGAGCGTTCA AAGCTGAAGA AATAGTCACA AAAGATTTCG ACAATCATCA GGACCTTCAC 
ACGTCCACCT GGTTCATATT TATAACCATT TCCCGAGCCT TCGGTCTGTA TATAGAGTGG 
TTCTGTCTGA TCTATGTAGG AATAATCACA ATCGCGTTCT TATTATTCGA GGATCTCGCT 
ATTGCCGGAG ACATAGGTTT GGTGATCACG CAGATCACCG CGGTGACTAA AATTTTGCAA 
TGGGGTATGA GGCAAACTGC CGAGTTAGAA AATCACATGA CTTCCATCGA GAGAGTTTTA 
GAGTACAGTC ATTTAGAGGA AGAACCCTTC TTAGACAGTA TACCGGAAAA GAAACCTCCC 
AAGGAGTGGC CCACTGCGGG CATGGTGGAA TTCAAAAATG TCAGATTAAA GTATGGACCG 
AAGAGTGCTT ACGTGTTGAA GGGTATAAAC TTCGTGGTGA AATCCAAAGA GAAAATTGGT 
GTCGTTGGGA GAACCGGTGC AGGGAAGACC TCATTGATCA GTGCTCTCTT TCGATTGGCA 
TACATAGAAG GAGAAATTAG CATAGACAAT ATACCCACAG ACACAGTTGC GTTGCACGAC 
TTCCGTTCAA AGATCAGTAT TATACCCCAA GAACCGGTGC TGTTTAGTGG TAGTCTTCGA 
AGAAATTTGG ATCCCTTTGA TGAGTATTCT GATAACGATT TATGGAGTGC GTTGCAAGAA 
GTAGAATTGA AGGAGACTAT CGCGGATATG CCAGCAGGTT TAACTAGCAA AGTAGCCGAG 
GAGGGATCAA ATTTTAGCGT TGGACAACGT CAATTATTGT GTCTTGTTAG AGCACTTGTT 
AGGAATAATA AGATTATGGT TCTCGATGAA GCCACTGCCA ATGTTGACTC GCAAACTGAT 
TCCATGATTC AAAAGACTGT TAGGAAGAAG TTTAAGCATT GTACTGTCTT CACTATAGCG 
CATAGATTAA ATACCATTAT GGATAGTGAT AAAATACTTG TGATGGACCA AGGTAACTTA 
ATGGAATTTG ATCATCCTTA TATTCTATTG CAAAAGAAAG GATATTTTTA CAATATGGTA 
CAACAAACAG GTCCTACAAT GGCAAATAAC TTATTTGAAA CAGCAAAGAA GTTTTTTTAC 
AAGAACAACG AAGCACTGTC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003705867.1
CDS	262..4125
Translation	MDKSERNLLKNPRQNANLLSILTFWWILKLFIVGYKKELEEEDLYCPLREDRSSYLGERIVKYWEIEVKRHEKKKDSPKPSLFRVLYKCFGKIVMNTGLALFVLEFGIRIIHPFLLARLLRYFAGNRKDWTSDVHYYAAAFCLLPLLDVLIMHWSLQTLTHVGMKIRVACCTLIYRKILRLSNSVLENETSAGQMVNFLSNDVNRLDYFVFSIHYLWIGPLQVFLIAYLIYREIGLGAITGMMTFLLCIPLQLYFGKRVSRLTLVSAQKTDNRLSLMNQIIAGVELIKMYVWEVPYSLLVEKARRKEVDVIKKYSIVEQIGLTCDIYVPRVSLFITILTYVLTGNTIDAEKVFMTTAFYAILQSSMTVGFTLSVHLLAEAMVSVRRLEKFMMHPEIIPPPKTQNQVATQSLPVYLKNVSARWDESRDCTLQNVDLSIQAGSFVAVIGQIGSGKSSLLKAILSELPLQDGILETSGKISFADQRPWIFASSIRQNILFGQPYNEKRYNEVIKVCQLKRDIDSFTHKDSTMVGERGINLSGGQRARINLARAIYAEADIYLLDDPLSAVDGHVGSHIVDECICGFLKGKTRILVTHQIQYLKPADQIVVINGGAVQAKGSFEELQRMSLDFMKIFKEVEESKTKEPETEEKQTIEETKKEEEPEVGSEPVEVAETRTVGKISAAVFLAYWKASKNPCLLAFMVVLFILSQVMASGADYLLAFWVNTEVASWVNIGNGTMAFQWNTLLSRDGIIYIYSALTFGFVCVYIVEAFTYYGVCMRASKNLHAAMFRSIVRATMYFFNTNPAGRILNRFSKDIGAIDKKLPFTTFDVTIMFLTFIGTVVIVGTVSAWLLIPTFVALLIFYYMRVIYIATSRSVKRMEGTTRSPVFDHVGATLQGLTTIRAFKAEEIVTKDFDNHQDLHTSTWFIFITISRAFGLYIEWFCLIYVGIITIAFLLFEDLAIAGDIGLVITQITAVTKILQWGMRQTAELENHMTSIERVLEYSHLEEEPFLDSIPEKKPPKEWPTAGMVEFKNVRLKYGPKSAYVLKGINFVVKSKEKIGVVGRTGAGKTSLISALFRLAYIEGEISIDNIPTDTVALHDFRSKISIIPQEPVLFSGSLRRNLDPFDEYSDNDLWSALQEVELKETIADMPAGLTSKVAEEGSNFSVGQRQLLCLVRALVRNNKIMVLDEATANVDSQTDSMIQKTVRKKFKHCTVFTIAHRLNTIMDSDKILVMDQGNLMEFDHPYILLQKKGYFYNMVQQTGPTMANNLFETAKKFFYKNNEALS

Target ORF information:

RefSeq Version	XM_003705819.2
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata probable multidrug resistance-associated protein lethal(2)03659 (LOC100877177), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003705819.2

ORF Insert Sequence:

ATGGATAAAA GTGAAAGGAA CCTATTGAAG AATCCGCGTC AAAATGCCAA TCTATTATCT 
ATTCTTACGT TCTGGTGGAT CCTCAAGTTG TTCATTGTCG GTTACAAAAA AGAATTGGAA 
GAAGAGGATT TGTATTGTCC ATTGAGAGAG GACAGGAGCA GTTACTTAGG CGAACGTATT 
GTAAAATATT GGGAGATTGA AGTGAAACGA CACGAGAAGA AGAAGGATAG CCCTAAACCA 
AGTTTATTTA GGGTATTGTA CAAATGCTTC GGTAAAATTG TCATGAACAC TGGACTAGCA 
CTGTTTGTTC TAGAATTCGG CATACGGATC ATACATCCTT TCCTTCTCGC TAGATTACTA 
CGTTACTTCG CTGGGAATAG AAAGGATTGG ACCAGTGATG TACATTACTA TGCAGCAGCA 
TTTTGTTTAC TACCTTTGCT AGATGTTCTG ATTATGCATT GGTCCTTACA AACCTTGACG 
CATGTCGGAA TGAAAATTAG AGTGGCTTGT TGTACCTTGA TTTATAGGAA GATATTGAGA 
CTATCCAATT CTGTCCTTGA AAATGAAACC TCTGCTGGGC AGATGGTTAA TTTCTTGAGT 
AACGACGTTA ATCGTCTCGA TTACTTCGTC TTTAGTATTC ATTATTTATG GATTGGACCT 
CTACAAGTAT TTCTAATAGC CTATTTAATA TATCGTGAGA TAGGCCTGGG AGCTATTACT 
GGGATGATGA CATTTTTATT ATGCATACCA TTACAGTTAT ATTTTGGAAA GAGAGTGTCA 
CGTTTGACTC TTGTATCGGC CCAGAAGACT GACAATAGAT TAAGTTTGAT GAACCAAATT 
ATAGCTGGTG TAGAATTAAT CAAGATGTAC GTTTGGGAGG TACCGTACTC TCTTCTCGTT 
GAAAAAGCTA GACGAAAAGA AGTGGATGTG ATCAAGAAAT ATTCGATTGT CGAGCAAATA 
GGTTTAACGT GTGATATCTA TGTTCCTCGA GTCAGCTTAT TTATTACGAT ATTAACCTAC 
GTTTTAACTG GCAACACCAT AGATGCCGAG AAAGTATTTA TGACTACTGC GTTTTACGCC 
ATTTTACAAA GCTCTATGAC CGTCGGTTTC ACTCTAAGTG TCCATCTGCT AGCTGAAGCC 
ATGGTGTCCG TCAGGCGTCT CGAGAAGTTC ATGATGCACC CCGAAATCAT TCCTCCGCCG 
AAGACGCAGA ATCAAGTAGC CACCCAATCA CTGCCCGTAT ACCTGAAAAA CGTGTCCGCC 
AGGTGGGACG AGTCCAGAGA CTGCACTTTA CAAAACGTAG ATTTATCGAT ACAAGCCGGA 
AGCTTCGTAG CGGTGATCGG ACAAATCGGT TCTGGAAAGA GTAGCCTCCT AAAAGCGATA 
CTCAGTGAGT TACCATTACA AGATGGCATC CTAGAAACCA GCGGTAAAAT AAGTTTCGCC 
GATCAACGAC CTTGGATATT TGCCTCCTCC ATCCGACAGA ACATACTGTT TGGTCAGCCT 
TACAACGAAA AGCGTTACAA CGAGGTGATT AAAGTCTGTC AACTAAAACG CGACATAGAT 
TCGTTTACGC ACAAAGACAG CACGATGGTC GGCGAAAGAG GGATCAATTT AAGTGGCGGT 
CAACGAGCTA GAATTAATTT GGCCCGTGCC ATCTACGCCG AGGCCGACAT ATATCTGTTG 
GATGATCCTC TATCGGCGGT CGACGGACAC GTGGGTAGCC ACATCGTGGA CGAGTGTATC 
TGCGGTTTCC TCAAGGGCAA GACTCGAATT CTAGTCACCC ATCAGATCCA GTACTTGAAA 
CCCGCCGATC AGATCGTCGT GATCAACGGC GGTGCCGTGC AAGCGAAGGG CAGCTTCGAG 
GAGCTTCAGA GGATGAGTCT GGACTTCATG AAGATCTTCA AAGAGGTGGA AGAGAGCAAG 
ACCAAAGAAC CCGAAACCGA GGAGAAGCAA ACGATCGAGG AGACGAAGAA AGAGGAAGAA 
CCGGAAGTAG GCAGTGAACC TGTCGAGGTG GCAGAAACGA GAACCGTAGG GAAAATTTCC 
GCCGCCGTTT TCTTAGCCTA CTGGAAAGCA AGTAAAAACC CGTGTCTGCT CGCTTTCATG 
GTGGTTCTGT TTATTTTGAG TCAAGTAATG GCGAGCGGCG CTGACTACTT GCTCGCGTTC 
TGGGTGAACA CCGAGGTAGC CTCGTGGGTT AATATCGGAA ATGGTACGAT GGCTTTTCAA 
TGGAATACTT TACTATCTCG CGATGGAATT ATTTATATTT ACAGCGCCTT AACGTTCGGC 
TTCGTCTGCG TCTATATCGT CGAAGCGTTC ACGTACTATG GGGTCTGCAT GAGAGCCTCG 
AAAAATTTGC ACGCGGCGAT GTTTCGTAGT ATTGTTCGGG CTACCATGTA CTTTTTCAAC 
ACTAATCCTG CTGGTCGTAT ATTAAACAGG TTTTCCAAAG ACATTGGTGC TATCGATAAG 
AAGCTTCCTT TCACGACGTT CGATGTCACA ATTATGTTCT TAACGTTTAT CGGAACTGTA 
GTAATTGTGG GTACTGTCAG CGCGTGGTTA TTGATACCGA CATTTGTCGC TCTACTCATT 
TTCTACTACA TGCGAGTTAT TTATATTGCA ACTAGTCGTT CTGTTAAACG TATGGAGGGT 
ACAACACGAT CGCCAGTGTT TGATCACGTC GGTGCAACTC TGCAGGGTCT CACGACAATC 
AGAGCGTTCA AAGCTGAAGA AATAGTCACA AAAGATTTCG ACAATCATCA GGACCTTCAC 
ACGTCCACCT GGTTCATATT TATAACCATT TCCCGAGCCT TCGGTCTGTA TATAGAGTGG 
TTCTGTCTGA TCTATGTAGG AATAATCACA ATCGCGTTCT TATTATTCGA GGATCTCGCT 
ATTGCCGGAG ACATAGGTTT GGTGATCACG CAGATCACCG CGGTGACTAA AATTTTGCAA 
TGGGGTATGA GGCAAACTGC CGAGTTAGAA AATCACATGA CTTCCATCGA GAGAGTTTTA 
GAGTACAGTC ATTTAGAGGA AGAACCCTTC TTAGACAGTA TACCGGAAAA GAAACCTCCC 
AAGGAGTGGC CCACTGCGGG CATGGTGGAA TTCAAAAATG TCAGATTAAA GTATGGACCG 
AAGAGTGCTT ACGTGTTGAA GGGTATAAAC TTCGTGGTGA AATCCAAAGA GAAAATTGGT 
GTCGTTGGGA GAACCGGTGC AGGGAAGACC TCATTGATCA GTGCTCTCTT TCGATTGGCA 
TACATAGAAG GAGAAATTAG CATAGACAAT ATACCCACAG ACACAGTTGC GTTGCACGAC 
TTCCGTTCAA AGATCAGTAT TATACCCCAA GAACCGGTGC TGTTTAGTGG TAGTCTTCGA 
AGAAATTTGG ATCCCTTTGA TGAGTATTCT GATAACGATT TATGGAGTGC GTTGCAAGAA 
GTAGAATTGA AGGAGACTAT CGCGGATATG CCAGCAGGTT TAACTAGCAA AGTAGCCGAG 
GAGGGATCAA ATTTTAGCGT TGGACAACGT CAATTATTGT GTCTTGTTAG AGCACTTGTT 
AGGAATAATA AGATTATGGT TCTCGATGAA GCCACTGCCA ATGTTGACTC GCAAACTGAT 
TCCATGATTC AAAAGACTGT TAGGAAGAAG TTTAAGCATT GTACTGTCTT CACTATAGCG 
CATAGATTAA ATACCATTAT GGATAGTGAT AAAATACTTG TGATGGACCA AGGTAACTTA 
ATGGAATTTG ATCATCCTTA TATTCTATTG CAAAAGAAAG GATATTTTTA CAATATGGTA 
CAACAAACAG GTCCTACAAT GGCAAATAAC TTATTTGAAA CAGCAAAGAA GTTTTTTTAC 
AAGAACAACG AAGCACTGTC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100877177 ( XM_012291340.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012146730.1 Megachile rotundata probable multidrug resistance-associated protein lethal(2)03659 (LOC100877177), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMe109917
Clone ID Related Accession (Same CDS sequence)	XM_003705819.2 , XM_012291340.1 , XM_012291338.1 , XM_012291339.1
Accession Version	XM_012291340.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	probable multidrug resistance-associated protein lethal(2)03659 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797352.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAA GTGAAAGGAA CCTATTGAAG AATCCGCGTC AAAATGCCAA TCTATTATCT 
ATTCTTACGT TCTGGTGGAT CCTCAAGTTG TTCATTGTCG GTTACAAAAA AGAATTGGAA 
GAAGAGGATT TGTATTGTCC ATTGAGAGAG GACAGGAGCA GTTACTTAGG CGAACGTATT 
GTAAAATATT GGGAGATTGA AGTGAAACGA CACGAGAAGA AGAAGGATAG CCCTAAACCA 
AGTTTATTTA GGGTATTGTA CAAATGCTTC GGTAAAATTG TCATGAACAC TGGACTAGCA 
CTGTTTGTTC TAGAATTCGG CATACGGATC ATACATCCTT TCCTTCTCGC TAGATTACTA 
CGTTACTTCG CTGGGAATAG AAAGGATTGG ACCAGTGATG TACATTACTA TGCAGCAGCA 
TTTTGTTTAC TACCTTTGCT AGATGTTCTG ATTATGCATT GGTCCTTACA AACCTTGACG 
CATGTCGGAA TGAAAATTAG AGTGGCTTGT TGTACCTTGA TTTATAGGAA GATATTGAGA 
CTATCCAATT CTGTCCTTGA AAATGAAACC TCTGCTGGGC AGATGGTTAA TTTCTTGAGT 
AACGACGTTA ATCGTCTCGA TTACTTCGTC TTTAGTATTC ATTATTTATG GATTGGACCT 
CTACAAGTAT TTCTAATAGC CTATTTAATA TATCGTGAGA TAGGCCTGGG AGCTATTACT 
GGGATGATGA CATTTTTATT ATGCATACCA TTACAGTTAT ATTTTGGAAA GAGAGTGTCA 
CGTTTGACTC TTGTATCGGC CCAGAAGACT GACAATAGAT TAAGTTTGAT GAACCAAATT 
ATAGCTGGTG TAGAATTAAT CAAGATGTAC GTTTGGGAGG TACCGTACTC TCTTCTCGTT 
GAAAAAGCTA GACGAAAAGA AGTGGATGTG ATCAAGAAAT ATTCGATTGT CGAGCAAATA 
GGTTTAACGT GTGATATCTA TGTTCCTCGA GTCAGCTTAT TTATTACGAT ATTAACCTAC 
GTTTTAACTG GCAACACCAT AGATGCCGAG AAAGTATTTA TGACTACTGC GTTTTACGCC 
ATTTTACAAA GCTCTATGAC CGTCGGTTTC ACTCTAAGTG TCCATCTGCT AGCTGAAGCC 
ATGGTGTCCG TCAGGCGTCT CGAGAAGTTC ATGATGCACC CCGAAATCAT TCCTCCGCCG 
AAGACGCAGA ATCAAGTAGC CACCCAATCA CTGCCCGTAT ACCTGAAAAA CGTGTCCGCC 
AGGTGGGACG AGTCCAGAGA CTGCACTTTA CAAAACGTAG ATTTATCGAT ACAAGCCGGA 
AGCTTCGTAG CGGTGATCGG ACAAATCGGT TCTGGAAAGA GTAGCCTCCT AAAAGCGATA 
CTCAGTGAGT TACCATTACA AGATGGCATC CTAGAAACCA GCGGTAAAAT AAGTTTCGCC 
GATCAACGAC CTTGGATATT TGCCTCCTCC ATCCGACAGA ACATACTGTT TGGTCAGCCT 
TACAACGAAA AGCGTTACAA CGAGGTGATT AAAGTCTGTC AACTAAAACG CGACATAGAT 
TCGTTTACGC ACAAAGACAG CACGATGGTC GGCGAAAGAG GGATCAATTT AAGTGGCGGT 
CAACGAGCTA GAATTAATTT GGCCCGTGCC ATCTACGCCG AGGCCGACAT ATATCTGTTG 
GATGATCCTC TATCGGCGGT CGACGGACAC GTGGGTAGCC ACATCGTGGA CGAGTGTATC 
TGCGGTTTCC TCAAGGGCAA GACTCGAATT CTAGTCACCC ATCAGATCCA GTACTTGAAA 
CCCGCCGATC AGATCGTCGT GATCAACGGC GGTGCCGTGC AAGCGAAGGG CAGCTTCGAG 
GAGCTTCAGA GGATGAGTCT GGACTTCATG AAGATCTTCA AAGAGGTGGA AGAGAGCAAG 
ACCAAAGAAC CCGAAACCGA GGAGAAGCAA ACGATCGAGG AGACGAAGAA AGAGGAAGAA 
CCGGAAGTAG GCAGTGAACC TGTCGAGGTG GCAGAAACGA GAACCGTAGG GAAAATTTCC 
GCCGCCGTTT TCTTAGCCTA CTGGAAAGCA AGTAAAAACC CGTGTCTGCT CGCTTTCATG 
GTGGTTCTGT TTATTTTGAG TCAAGTAATG GCGAGCGGCG CTGACTACTT GCTCGCGTTC 
TGGGTGAACA CCGAGGTAGC CTCGTGGGTT AATATCGGAA ATGGTACGAT GGCTTTTCAA 
TGGAATACTT TACTATCTCG CGATGGAATT ATTTATATTT ACAGCGCCTT AACGTTCGGC 
TTCGTCTGCG TCTATATCGT CGAAGCGTTC ACGTACTATG GGGTCTGCAT GAGAGCCTCG 
AAAAATTTGC ACGCGGCGAT GTTTCGTAGT ATTGTTCGGG CTACCATGTA CTTTTTCAAC 
ACTAATCCTG CTGGTCGTAT ATTAAACAGG TTTTCCAAAG ACATTGGTGC TATCGATAAG 
AAGCTTCCTT TCACGACGTT CGATGTCACA ATTATGTTCT TAACGTTTAT CGGAACTGTA 
GTAATTGTGG GTACTGTCAG CGCGTGGTTA TTGATACCGA CATTTGTCGC TCTACTCATT 
TTCTACTACA TGCGAGTTAT TTATATTGCA ACTAGTCGTT CTGTTAAACG TATGGAGGGT 
ACAACACGAT CGCCAGTGTT TGATCACGTC GGTGCAACTC TGCAGGGTCT CACGACAATC 
AGAGCGTTCA AAGCTGAAGA AATAGTCACA AAAGATTTCG ACAATCATCA GGACCTTCAC 
ACGTCCACCT GGTTCATATT TATAACCATT TCCCGAGCCT TCGGTCTGTA TATAGAGTGG 
TTCTGTCTGA TCTATGTAGG AATAATCACA ATCGCGTTCT TATTATTCGA GGATCTCGCT 
ATTGCCGGAG ACATAGGTTT GGTGATCACG CAGATCACCG CGGTGACTAA AATTTTGCAA 
TGGGGTATGA GGCAAACTGC CGAGTTAGAA AATCACATGA CTTCCATCGA GAGAGTTTTA 
GAGTACAGTC ATTTAGAGGA AGAACCCTTC TTAGACAGTA TACCGGAAAA GAAACCTCCC 
AAGGAGTGGC CCACTGCGGG CATGGTGGAA TTCAAAAATG TCAGATTAAA GTATGGACCG 
AAGAGTGCTT ACGTGTTGAA GGGTATAAAC TTCGTGGTGA AATCCAAAGA GAAAATTGGT 
GTCGTTGGGA GAACCGGTGC AGGGAAGACC TCATTGATCA GTGCTCTCTT TCGATTGGCA 
TACATAGAAG GAGAAATTAG CATAGACAAT ATACCCACAG ACACAGTTGC GTTGCACGAC 
TTCCGTTCAA AGATCAGTAT TATACCCCAA GAACCGGTGC TGTTTAGTGG TAGTCTTCGA 
AGAAATTTGG ATCCCTTTGA TGAGTATTCT GATAACGATT TATGGAGTGC GTTGCAAGAA 
GTAGAATTGA AGGAGACTAT CGCGGATATG CCAGCAGGTT TAACTAGCAA AGTAGCCGAG 
GAGGGATCAA ATTTTAGCGT TGGACAACGT CAATTATTGT GTCTTGTTAG AGCACTTGTT 
AGGAATAATA AGATTATGGT TCTCGATGAA GCCACTGCCA ATGTTGACTC GCAAACTGAT 
TCCATGATTC AAAAGACTGT TAGGAAGAAG TTTAAGCATT GTACTGTCTT CACTATAGCG 
CATAGATTAA ATACCATTAT GGATAGTGAT AAAATACTTG TGATGGACCA AGGTAACTTA 
ATGGAATTTG ATCATCCTTA TATTCTATTG CAAAAGAAAG GATATTTTTA CAATATGGTA 
CAACAAACAG GTCCTACAAT GGCAAATAAC TTATTTGAAA CAGCAAAGAA GTTTTTTTAC 
AAGAACAACG AAGCACTGTC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012146730.1
CDS	142..4005
Translation	MDKSERNLLKNPRQNANLLSILTFWWILKLFIVGYKKELEEEDLYCPLREDRSSYLGERIVKYWEIEVKRHEKKKDSPKPSLFRVLYKCFGKIVMNTGLALFVLEFGIRIIHPFLLARLLRYFAGNRKDWTSDVHYYAAAFCLLPLLDVLIMHWSLQTLTHVGMKIRVACCTLIYRKILRLSNSVLENETSAGQMVNFLSNDVNRLDYFVFSIHYLWIGPLQVFLIAYLIYREIGLGAITGMMTFLLCIPLQLYFGKRVSRLTLVSAQKTDNRLSLMNQIIAGVELIKMYVWEVPYSLLVEKARRKEVDVIKKYSIVEQIGLTCDIYVPRVSLFITILTYVLTGNTIDAEKVFMTTAFYAILQSSMTVGFTLSVHLLAEAMVSVRRLEKFMMHPEIIPPPKTQNQVATQSLPVYLKNVSARWDESRDCTLQNVDLSIQAGSFVAVIGQIGSGKSSLLKAILSELPLQDGILETSGKISFADQRPWIFASSIRQNILFGQPYNEKRYNEVIKVCQLKRDIDSFTHKDSTMVGERGINLSGGQRARINLARAIYAEADIYLLDDPLSAVDGHVGSHIVDECICGFLKGKTRILVTHQIQYLKPADQIVVINGGAVQAKGSFEELQRMSLDFMKIFKEVEESKTKEPETEEKQTIEETKKEEEPEVGSEPVEVAETRTVGKISAAVFLAYWKASKNPCLLAFMVVLFILSQVMASGADYLLAFWVNTEVASWVNIGNGTMAFQWNTLLSRDGIIYIYSALTFGFVCVYIVEAFTYYGVCMRASKNLHAAMFRSIVRATMYFFNTNPAGRILNRFSKDIGAIDKKLPFTTFDVTIMFLTFIGTVVIVGTVSAWLLIPTFVALLIFYYMRVIYIATSRSVKRMEGTTRSPVFDHVGATLQGLTTIRAFKAEEIVTKDFDNHQDLHTSTWFIFITISRAFGLYIEWFCLIYVGIITIAFLLFEDLAIAGDIGLVITQITAVTKILQWGMRQTAELENHMTSIERVLEYSHLEEEPFLDSIPEKKPPKEWPTAGMVEFKNVRLKYGPKSAYVLKGINFVVKSKEKIGVVGRTGAGKTSLISALFRLAYIEGEISIDNIPTDTVALHDFRSKISIIPQEPVLFSGSLRRNLDPFDEYSDNDLWSALQEVELKETIADMPAGLTSKVAEEGSNFSVGQRQLLCLVRALVRNNKIMVLDEATANVDSQTDSMIQKTVRKKFKHCTVFTIAHRLNTIMDSDKILVMDQGNLMEFDHPYILLQKKGYFYNMVQQTGPTMANNLFETAKKFFYKNNEALS

Target ORF information:

RefSeq Version	XM_012291340.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata probable multidrug resistance-associated protein lethal(2)03659 (LOC100877177), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012291340.1

ORF Insert Sequence:

ATGGATAAAA GTGAAAGGAA CCTATTGAAG AATCCGCGTC AAAATGCCAA TCTATTATCT 
ATTCTTACGT TCTGGTGGAT CCTCAAGTTG TTCATTGTCG GTTACAAAAA AGAATTGGAA 
GAAGAGGATT TGTATTGTCC ATTGAGAGAG GACAGGAGCA GTTACTTAGG CGAACGTATT 
GTAAAATATT GGGAGATTGA AGTGAAACGA CACGAGAAGA AGAAGGATAG CCCTAAACCA 
AGTTTATTTA GGGTATTGTA CAAATGCTTC GGTAAAATTG TCATGAACAC TGGACTAGCA 
CTGTTTGTTC TAGAATTCGG CATACGGATC ATACATCCTT TCCTTCTCGC TAGATTACTA 
CGTTACTTCG CTGGGAATAG AAAGGATTGG ACCAGTGATG TACATTACTA TGCAGCAGCA 
TTTTGTTTAC TACCTTTGCT AGATGTTCTG ATTATGCATT GGTCCTTACA AACCTTGACG 
CATGTCGGAA TGAAAATTAG AGTGGCTTGT TGTACCTTGA TTTATAGGAA GATATTGAGA 
CTATCCAATT CTGTCCTTGA AAATGAAACC TCTGCTGGGC AGATGGTTAA TTTCTTGAGT 
AACGACGTTA ATCGTCTCGA TTACTTCGTC TTTAGTATTC ATTATTTATG GATTGGACCT 
CTACAAGTAT TTCTAATAGC CTATTTAATA TATCGTGAGA TAGGCCTGGG AGCTATTACT 
GGGATGATGA CATTTTTATT ATGCATACCA TTACAGTTAT ATTTTGGAAA GAGAGTGTCA 
CGTTTGACTC TTGTATCGGC CCAGAAGACT GACAATAGAT TAAGTTTGAT GAACCAAATT 
ATAGCTGGTG TAGAATTAAT CAAGATGTAC GTTTGGGAGG TACCGTACTC TCTTCTCGTT 
GAAAAAGCTA GACGAAAAGA AGTGGATGTG ATCAAGAAAT ATTCGATTGT CGAGCAAATA 
GGTTTAACGT GTGATATCTA TGTTCCTCGA GTCAGCTTAT TTATTACGAT ATTAACCTAC 
GTTTTAACTG GCAACACCAT AGATGCCGAG AAAGTATTTA TGACTACTGC GTTTTACGCC 
ATTTTACAAA GCTCTATGAC CGTCGGTTTC ACTCTAAGTG TCCATCTGCT AGCTGAAGCC 
ATGGTGTCCG TCAGGCGTCT CGAGAAGTTC ATGATGCACC CCGAAATCAT TCCTCCGCCG 
AAGACGCAGA ATCAAGTAGC CACCCAATCA CTGCCCGTAT ACCTGAAAAA CGTGTCCGCC 
AGGTGGGACG AGTCCAGAGA CTGCACTTTA CAAAACGTAG ATTTATCGAT ACAAGCCGGA 
AGCTTCGTAG CGGTGATCGG ACAAATCGGT TCTGGAAAGA GTAGCCTCCT AAAAGCGATA 
CTCAGTGAGT TACCATTACA AGATGGCATC CTAGAAACCA GCGGTAAAAT AAGTTTCGCC 
GATCAACGAC CTTGGATATT TGCCTCCTCC ATCCGACAGA ACATACTGTT TGGTCAGCCT 
TACAACGAAA AGCGTTACAA CGAGGTGATT AAAGTCTGTC AACTAAAACG CGACATAGAT 
TCGTTTACGC ACAAAGACAG CACGATGGTC GGCGAAAGAG GGATCAATTT AAGTGGCGGT 
CAACGAGCTA GAATTAATTT GGCCCGTGCC ATCTACGCCG AGGCCGACAT ATATCTGTTG 
GATGATCCTC TATCGGCGGT CGACGGACAC GTGGGTAGCC ACATCGTGGA CGAGTGTATC 
TGCGGTTTCC TCAAGGGCAA GACTCGAATT CTAGTCACCC ATCAGATCCA GTACTTGAAA 
CCCGCCGATC AGATCGTCGT GATCAACGGC GGTGCCGTGC AAGCGAAGGG CAGCTTCGAG 
GAGCTTCAGA GGATGAGTCT GGACTTCATG AAGATCTTCA AAGAGGTGGA AGAGAGCAAG 
ACCAAAGAAC CCGAAACCGA GGAGAAGCAA ACGATCGAGG AGACGAAGAA AGAGGAAGAA 
CCGGAAGTAG GCAGTGAACC TGTCGAGGTG GCAGAAACGA GAACCGTAGG GAAAATTTCC 
GCCGCCGTTT TCTTAGCCTA CTGGAAAGCA AGTAAAAACC CGTGTCTGCT CGCTTTCATG 
GTGGTTCTGT TTATTTTGAG TCAAGTAATG GCGAGCGGCG CTGACTACTT GCTCGCGTTC 
TGGGTGAACA CCGAGGTAGC CTCGTGGGTT AATATCGGAA ATGGTACGAT GGCTTTTCAA 
TGGAATACTT TACTATCTCG CGATGGAATT ATTTATATTT ACAGCGCCTT AACGTTCGGC 
TTCGTCTGCG TCTATATCGT CGAAGCGTTC ACGTACTATG GGGTCTGCAT GAGAGCCTCG 
AAAAATTTGC ACGCGGCGAT GTTTCGTAGT ATTGTTCGGG CTACCATGTA CTTTTTCAAC 
ACTAATCCTG CTGGTCGTAT ATTAAACAGG TTTTCCAAAG ACATTGGTGC TATCGATAAG 
AAGCTTCCTT TCACGACGTT CGATGTCACA ATTATGTTCT TAACGTTTAT CGGAACTGTA 
GTAATTGTGG GTACTGTCAG CGCGTGGTTA TTGATACCGA CATTTGTCGC TCTACTCATT 
TTCTACTACA TGCGAGTTAT TTATATTGCA ACTAGTCGTT CTGTTAAACG TATGGAGGGT 
ACAACACGAT CGCCAGTGTT TGATCACGTC GGTGCAACTC TGCAGGGTCT CACGACAATC 
AGAGCGTTCA AAGCTGAAGA AATAGTCACA AAAGATTTCG ACAATCATCA GGACCTTCAC 
ACGTCCACCT GGTTCATATT TATAACCATT TCCCGAGCCT TCGGTCTGTA TATAGAGTGG 
TTCTGTCTGA TCTATGTAGG AATAATCACA ATCGCGTTCT TATTATTCGA GGATCTCGCT 
ATTGCCGGAG ACATAGGTTT GGTGATCACG CAGATCACCG CGGTGACTAA AATTTTGCAA 
TGGGGTATGA GGCAAACTGC CGAGTTAGAA AATCACATGA CTTCCATCGA GAGAGTTTTA 
GAGTACAGTC ATTTAGAGGA AGAACCCTTC TTAGACAGTA TACCGGAAAA GAAACCTCCC 
AAGGAGTGGC CCACTGCGGG CATGGTGGAA TTCAAAAATG TCAGATTAAA GTATGGACCG 
AAGAGTGCTT ACGTGTTGAA GGGTATAAAC TTCGTGGTGA AATCCAAAGA GAAAATTGGT 
GTCGTTGGGA GAACCGGTGC AGGGAAGACC TCATTGATCA GTGCTCTCTT TCGATTGGCA 
TACATAGAAG GAGAAATTAG CATAGACAAT ATACCCACAG ACACAGTTGC GTTGCACGAC 
TTCCGTTCAA AGATCAGTAT TATACCCCAA GAACCGGTGC TGTTTAGTGG TAGTCTTCGA 
AGAAATTTGG ATCCCTTTGA TGAGTATTCT GATAACGATT TATGGAGTGC GTTGCAAGAA 
GTAGAATTGA AGGAGACTAT CGCGGATATG CCAGCAGGTT TAACTAGCAA AGTAGCCGAG 
GAGGGATCAA ATTTTAGCGT TGGACAACGT CAATTATTGT GTCTTGTTAG AGCACTTGTT 
AGGAATAATA AGATTATGGT TCTCGATGAA GCCACTGCCA ATGTTGACTC GCAAACTGAT 
TCCATGATTC AAAAGACTGT TAGGAAGAAG TTTAAGCATT GTACTGTCTT CACTATAGCG 
CATAGATTAA ATACCATTAT GGATAGTGAT AAAATACTTG TGATGGACCA AGGTAACTTA 
ATGGAATTTG ATCATCCTTA TATTCTATTG CAAAAGAAAG GATATTTTTA CAATATGGTA 
CAACAAACAG GTCCTACAAT GGCAAATAAC TTATTTGAAA CAGCAAAGAA GTTTTTTTAC 
AAGAACAACG AAGCACTGTC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100877177 ( XM_012291338.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012146728.1 Megachile rotundata probable multidrug resistance-associated protein lethal(2)03659 (LOC100877177), transcript variant X2, mRNA.

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CloneID	OMe109917
Clone ID Related Accession (Same CDS sequence)	XM_003705819.2 , XM_012291340.1 , XM_012291338.1 , XM_012291339.1
Accession Version	XM_012291338.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	probable multidrug resistance-associated protein lethal(2)03659 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797352.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAA GTGAAAGGAA CCTATTGAAG AATCCGCGTC AAAATGCCAA TCTATTATCT 
ATTCTTACGT TCTGGTGGAT CCTCAAGTTG TTCATTGTCG GTTACAAAAA AGAATTGGAA 
GAAGAGGATT TGTATTGTCC ATTGAGAGAG GACAGGAGCA GTTACTTAGG CGAACGTATT 
GTAAAATATT GGGAGATTGA AGTGAAACGA CACGAGAAGA AGAAGGATAG CCCTAAACCA 
AGTTTATTTA GGGTATTGTA CAAATGCTTC GGTAAAATTG TCATGAACAC TGGACTAGCA 
CTGTTTGTTC TAGAATTCGG CATACGGATC ATACATCCTT TCCTTCTCGC TAGATTACTA 
CGTTACTTCG CTGGGAATAG AAAGGATTGG ACCAGTGATG TACATTACTA TGCAGCAGCA 
TTTTGTTTAC TACCTTTGCT AGATGTTCTG ATTATGCATT GGTCCTTACA AACCTTGACG 
CATGTCGGAA TGAAAATTAG AGTGGCTTGT TGTACCTTGA TTTATAGGAA GATATTGAGA 
CTATCCAATT CTGTCCTTGA AAATGAAACC TCTGCTGGGC AGATGGTTAA TTTCTTGAGT 
AACGACGTTA ATCGTCTCGA TTACTTCGTC TTTAGTATTC ATTATTTATG GATTGGACCT 
CTACAAGTAT TTCTAATAGC CTATTTAATA TATCGTGAGA TAGGCCTGGG AGCTATTACT 
GGGATGATGA CATTTTTATT ATGCATACCA TTACAGTTAT ATTTTGGAAA GAGAGTGTCA 
CGTTTGACTC TTGTATCGGC CCAGAAGACT GACAATAGAT TAAGTTTGAT GAACCAAATT 
ATAGCTGGTG TAGAATTAAT CAAGATGTAC GTTTGGGAGG TACCGTACTC TCTTCTCGTT 
GAAAAAGCTA GACGAAAAGA AGTGGATGTG ATCAAGAAAT ATTCGATTGT CGAGCAAATA 
GGTTTAACGT GTGATATCTA TGTTCCTCGA GTCAGCTTAT TTATTACGAT ATTAACCTAC 
GTTTTAACTG GCAACACCAT AGATGCCGAG AAAGTATTTA TGACTACTGC GTTTTACGCC 
ATTTTACAAA GCTCTATGAC CGTCGGTTTC ACTCTAAGTG TCCATCTGCT AGCTGAAGCC 
ATGGTGTCCG TCAGGCGTCT CGAGAAGTTC ATGATGCACC CCGAAATCAT TCCTCCGCCG 
AAGACGCAGA ATCAAGTAGC CACCCAATCA CTGCCCGTAT ACCTGAAAAA CGTGTCCGCC 
AGGTGGGACG AGTCCAGAGA CTGCACTTTA CAAAACGTAG ATTTATCGAT ACAAGCCGGA 
AGCTTCGTAG CGGTGATCGG ACAAATCGGT TCTGGAAAGA GTAGCCTCCT AAAAGCGATA 
CTCAGTGAGT TACCATTACA AGATGGCATC CTAGAAACCA GCGGTAAAAT AAGTTTCGCC 
GATCAACGAC CTTGGATATT TGCCTCCTCC ATCCGACAGA ACATACTGTT TGGTCAGCCT 
TACAACGAAA AGCGTTACAA CGAGGTGATT AAAGTCTGTC AACTAAAACG CGACATAGAT 
TCGTTTACGC ACAAAGACAG CACGATGGTC GGCGAAAGAG GGATCAATTT AAGTGGCGGT 
CAACGAGCTA GAATTAATTT GGCCCGTGCC ATCTACGCCG AGGCCGACAT ATATCTGTTG 
GATGATCCTC TATCGGCGGT CGACGGACAC GTGGGTAGCC ACATCGTGGA CGAGTGTATC 
TGCGGTTTCC TCAAGGGCAA GACTCGAATT CTAGTCACCC ATCAGATCCA GTACTTGAAA 
CCCGCCGATC AGATCGTCGT GATCAACGGC GGTGCCGTGC AAGCGAAGGG CAGCTTCGAG 
GAGCTTCAGA GGATGAGTCT GGACTTCATG AAGATCTTCA AAGAGGTGGA AGAGAGCAAG 
ACCAAAGAAC CCGAAACCGA GGAGAAGCAA ACGATCGAGG AGACGAAGAA AGAGGAAGAA 
CCGGAAGTAG GCAGTGAACC TGTCGAGGTG GCAGAAACGA GAACCGTAGG GAAAATTTCC 
GCCGCCGTTT TCTTAGCCTA CTGGAAAGCA AGTAAAAACC CGTGTCTGCT CGCTTTCATG 
GTGGTTCTGT TTATTTTGAG TCAAGTAATG GCGAGCGGCG CTGACTACTT GCTCGCGTTC 
TGGGTGAACA CCGAGGTAGC CTCGTGGGTT AATATCGGAA ATGGTACGAT GGCTTTTCAA 
TGGAATACTT TACTATCTCG CGATGGAATT ATTTATATTT ACAGCGCCTT AACGTTCGGC 
TTCGTCTGCG TCTATATCGT CGAAGCGTTC ACGTACTATG GGGTCTGCAT GAGAGCCTCG 
AAAAATTTGC ACGCGGCGAT GTTTCGTAGT ATTGTTCGGG CTACCATGTA CTTTTTCAAC 
ACTAATCCTG CTGGTCGTAT ATTAAACAGG TTTTCCAAAG ACATTGGTGC TATCGATAAG 
AAGCTTCCTT TCACGACGTT CGATGTCACA ATTATGTTCT TAACGTTTAT CGGAACTGTA 
GTAATTGTGG GTACTGTCAG CGCGTGGTTA TTGATACCGA CATTTGTCGC TCTACTCATT 
TTCTACTACA TGCGAGTTAT TTATATTGCA ACTAGTCGTT CTGTTAAACG TATGGAGGGT 
ACAACACGAT CGCCAGTGTT TGATCACGTC GGTGCAACTC TGCAGGGTCT CACGACAATC 
AGAGCGTTCA AAGCTGAAGA AATAGTCACA AAAGATTTCG ACAATCATCA GGACCTTCAC 
ACGTCCACCT GGTTCATATT TATAACCATT TCCCGAGCCT TCGGTCTGTA TATAGAGTGG 
TTCTGTCTGA TCTATGTAGG AATAATCACA ATCGCGTTCT TATTATTCGA GGATCTCGCT 
ATTGCCGGAG ACATAGGTTT GGTGATCACG CAGATCACCG CGGTGACTAA AATTTTGCAA 
TGGGGTATGA GGCAAACTGC CGAGTTAGAA AATCACATGA CTTCCATCGA GAGAGTTTTA 
GAGTACAGTC ATTTAGAGGA AGAACCCTTC TTAGACAGTA TACCGGAAAA GAAACCTCCC 
AAGGAGTGGC CCACTGCGGG CATGGTGGAA TTCAAAAATG TCAGATTAAA GTATGGACCG 
AAGAGTGCTT ACGTGTTGAA GGGTATAAAC TTCGTGGTGA AATCCAAAGA GAAAATTGGT 
GTCGTTGGGA GAACCGGTGC AGGGAAGACC TCATTGATCA GTGCTCTCTT TCGATTGGCA 
TACATAGAAG GAGAAATTAG CATAGACAAT ATACCCACAG ACACAGTTGC GTTGCACGAC 
TTCCGTTCAA AGATCAGTAT TATACCCCAA GAACCGGTGC TGTTTAGTGG TAGTCTTCGA 
AGAAATTTGG ATCCCTTTGA TGAGTATTCT GATAACGATT TATGGAGTGC GTTGCAAGAA 
GTAGAATTGA AGGAGACTAT CGCGGATATG CCAGCAGGTT TAACTAGCAA AGTAGCCGAG 
GAGGGATCAA ATTTTAGCGT TGGACAACGT CAATTATTGT GTCTTGTTAG AGCACTTGTT 
AGGAATAATA AGATTATGGT TCTCGATGAA GCCACTGCCA ATGTTGACTC GCAAACTGAT 
TCCATGATTC AAAAGACTGT TAGGAAGAAG TTTAAGCATT GTACTGTCTT CACTATAGCG 
CATAGATTAA ATACCATTAT GGATAGTGAT AAAATACTTG TGATGGACCA AGGTAACTTA 
ATGGAATTTG ATCATCCTTA TATTCTATTG CAAAAGAAAG GATATTTTTA CAATATGGTA 
CAACAAACAG GTCCTACAAT GGCAAATAAC TTATTTGAAA CAGCAAAGAA GTTTTTTTAC 
AAGAACAACG AAGCACTGTC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012146728.1
CDS	405..4268
Translation	MDKSERNLLKNPRQNANLLSILTFWWILKLFIVGYKKELEEEDLYCPLREDRSSYLGERIVKYWEIEVKRHEKKKDSPKPSLFRVLYKCFGKIVMNTGLALFVLEFGIRIIHPFLLARLLRYFAGNRKDWTSDVHYYAAAFCLLPLLDVLIMHWSLQTLTHVGMKIRVACCTLIYRKILRLSNSVLENETSAGQMVNFLSNDVNRLDYFVFSIHYLWIGPLQVFLIAYLIYREIGLGAITGMMTFLLCIPLQLYFGKRVSRLTLVSAQKTDNRLSLMNQIIAGVELIKMYVWEVPYSLLVEKARRKEVDVIKKYSIVEQIGLTCDIYVPRVSLFITILTYVLTGNTIDAEKVFMTTAFYAILQSSMTVGFTLSVHLLAEAMVSVRRLEKFMMHPEIIPPPKTQNQVATQSLPVYLKNVSARWDESRDCTLQNVDLSIQAGSFVAVIGQIGSGKSSLLKAILSELPLQDGILETSGKISFADQRPWIFASSIRQNILFGQPYNEKRYNEVIKVCQLKRDIDSFTHKDSTMVGERGINLSGGQRARINLARAIYAEADIYLLDDPLSAVDGHVGSHIVDECICGFLKGKTRILVTHQIQYLKPADQIVVINGGAVQAKGSFEELQRMSLDFMKIFKEVEESKTKEPETEEKQTIEETKKEEEPEVGSEPVEVAETRTVGKISAAVFLAYWKASKNPCLLAFMVVLFILSQVMASGADYLLAFWVNTEVASWVNIGNGTMAFQWNTLLSRDGIIYIYSALTFGFVCVYIVEAFTYYGVCMRASKNLHAAMFRSIVRATMYFFNTNPAGRILNRFSKDIGAIDKKLPFTTFDVTIMFLTFIGTVVIVGTVSAWLLIPTFVALLIFYYMRVIYIATSRSVKRMEGTTRSPVFDHVGATLQGLTTIRAFKAEEIVTKDFDNHQDLHTSTWFIFITISRAFGLYIEWFCLIYVGIITIAFLLFEDLAIAGDIGLVITQITAVTKILQWGMRQTAELENHMTSIERVLEYSHLEEEPFLDSIPEKKPPKEWPTAGMVEFKNVRLKYGPKSAYVLKGINFVVKSKEKIGVVGRTGAGKTSLISALFRLAYIEGEISIDNIPTDTVALHDFRSKISIIPQEPVLFSGSLRRNLDPFDEYSDNDLWSALQEVELKETIADMPAGLTSKVAEEGSNFSVGQRQLLCLVRALVRNNKIMVLDEATANVDSQTDSMIQKTVRKKFKHCTVFTIAHRLNTIMDSDKILVMDQGNLMEFDHPYILLQKKGYFYNMVQQTGPTMANNLFETAKKFFYKNNEALS

Target ORF information:

RefSeq Version	XM_012291338.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata probable multidrug resistance-associated protein lethal(2)03659 (LOC100877177), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012291338.1

ORF Insert Sequence:

ATGGATAAAA GTGAAAGGAA CCTATTGAAG AATCCGCGTC AAAATGCCAA TCTATTATCT 
ATTCTTACGT TCTGGTGGAT CCTCAAGTTG TTCATTGTCG GTTACAAAAA AGAATTGGAA 
GAAGAGGATT TGTATTGTCC ATTGAGAGAG GACAGGAGCA GTTACTTAGG CGAACGTATT 
GTAAAATATT GGGAGATTGA AGTGAAACGA CACGAGAAGA AGAAGGATAG CCCTAAACCA 
AGTTTATTTA GGGTATTGTA CAAATGCTTC GGTAAAATTG TCATGAACAC TGGACTAGCA 
CTGTTTGTTC TAGAATTCGG CATACGGATC ATACATCCTT TCCTTCTCGC TAGATTACTA 
CGTTACTTCG CTGGGAATAG AAAGGATTGG ACCAGTGATG TACATTACTA TGCAGCAGCA 
TTTTGTTTAC TACCTTTGCT AGATGTTCTG ATTATGCATT GGTCCTTACA AACCTTGACG 
CATGTCGGAA TGAAAATTAG AGTGGCTTGT TGTACCTTGA TTTATAGGAA GATATTGAGA 
CTATCCAATT CTGTCCTTGA AAATGAAACC TCTGCTGGGC AGATGGTTAA TTTCTTGAGT 
AACGACGTTA ATCGTCTCGA TTACTTCGTC TTTAGTATTC ATTATTTATG GATTGGACCT 
CTACAAGTAT TTCTAATAGC CTATTTAATA TATCGTGAGA TAGGCCTGGG AGCTATTACT 
GGGATGATGA CATTTTTATT ATGCATACCA TTACAGTTAT ATTTTGGAAA GAGAGTGTCA 
CGTTTGACTC TTGTATCGGC CCAGAAGACT GACAATAGAT TAAGTTTGAT GAACCAAATT 
ATAGCTGGTG TAGAATTAAT CAAGATGTAC GTTTGGGAGG TACCGTACTC TCTTCTCGTT 
GAAAAAGCTA GACGAAAAGA AGTGGATGTG ATCAAGAAAT ATTCGATTGT CGAGCAAATA 
GGTTTAACGT GTGATATCTA TGTTCCTCGA GTCAGCTTAT TTATTACGAT ATTAACCTAC 
GTTTTAACTG GCAACACCAT AGATGCCGAG AAAGTATTTA TGACTACTGC GTTTTACGCC 
ATTTTACAAA GCTCTATGAC CGTCGGTTTC ACTCTAAGTG TCCATCTGCT AGCTGAAGCC 
ATGGTGTCCG TCAGGCGTCT CGAGAAGTTC ATGATGCACC CCGAAATCAT TCCTCCGCCG 
AAGACGCAGA ATCAAGTAGC CACCCAATCA CTGCCCGTAT ACCTGAAAAA CGTGTCCGCC 
AGGTGGGACG AGTCCAGAGA CTGCACTTTA CAAAACGTAG ATTTATCGAT ACAAGCCGGA 
AGCTTCGTAG CGGTGATCGG ACAAATCGGT TCTGGAAAGA GTAGCCTCCT AAAAGCGATA 
CTCAGTGAGT TACCATTACA AGATGGCATC CTAGAAACCA GCGGTAAAAT AAGTTTCGCC 
GATCAACGAC CTTGGATATT TGCCTCCTCC ATCCGACAGA ACATACTGTT TGGTCAGCCT 
TACAACGAAA AGCGTTACAA CGAGGTGATT AAAGTCTGTC AACTAAAACG CGACATAGAT 
TCGTTTACGC ACAAAGACAG CACGATGGTC GGCGAAAGAG GGATCAATTT AAGTGGCGGT 
CAACGAGCTA GAATTAATTT GGCCCGTGCC ATCTACGCCG AGGCCGACAT ATATCTGTTG 
GATGATCCTC TATCGGCGGT CGACGGACAC GTGGGTAGCC ACATCGTGGA CGAGTGTATC 
TGCGGTTTCC TCAAGGGCAA GACTCGAATT CTAGTCACCC ATCAGATCCA GTACTTGAAA 
CCCGCCGATC AGATCGTCGT GATCAACGGC GGTGCCGTGC AAGCGAAGGG CAGCTTCGAG 
GAGCTTCAGA GGATGAGTCT GGACTTCATG AAGATCTTCA AAGAGGTGGA AGAGAGCAAG 
ACCAAAGAAC CCGAAACCGA GGAGAAGCAA ACGATCGAGG AGACGAAGAA AGAGGAAGAA 
CCGGAAGTAG GCAGTGAACC TGTCGAGGTG GCAGAAACGA GAACCGTAGG GAAAATTTCC 
GCCGCCGTTT TCTTAGCCTA CTGGAAAGCA AGTAAAAACC CGTGTCTGCT CGCTTTCATG 
GTGGTTCTGT TTATTTTGAG TCAAGTAATG GCGAGCGGCG CTGACTACTT GCTCGCGTTC 
TGGGTGAACA CCGAGGTAGC CTCGTGGGTT AATATCGGAA ATGGTACGAT GGCTTTTCAA 
TGGAATACTT TACTATCTCG CGATGGAATT ATTTATATTT ACAGCGCCTT AACGTTCGGC 
TTCGTCTGCG TCTATATCGT CGAAGCGTTC ACGTACTATG GGGTCTGCAT GAGAGCCTCG 
AAAAATTTGC ACGCGGCGAT GTTTCGTAGT ATTGTTCGGG CTACCATGTA CTTTTTCAAC 
ACTAATCCTG CTGGTCGTAT ATTAAACAGG TTTTCCAAAG ACATTGGTGC TATCGATAAG 
AAGCTTCCTT TCACGACGTT CGATGTCACA ATTATGTTCT TAACGTTTAT CGGAACTGTA 
GTAATTGTGG GTACTGTCAG CGCGTGGTTA TTGATACCGA CATTTGTCGC TCTACTCATT 
TTCTACTACA TGCGAGTTAT TTATATTGCA ACTAGTCGTT CTGTTAAACG TATGGAGGGT 
ACAACACGAT CGCCAGTGTT TGATCACGTC GGTGCAACTC TGCAGGGTCT CACGACAATC 
AGAGCGTTCA AAGCTGAAGA AATAGTCACA AAAGATTTCG ACAATCATCA GGACCTTCAC 
ACGTCCACCT GGTTCATATT TATAACCATT TCCCGAGCCT TCGGTCTGTA TATAGAGTGG 
TTCTGTCTGA TCTATGTAGG AATAATCACA ATCGCGTTCT TATTATTCGA GGATCTCGCT 
ATTGCCGGAG ACATAGGTTT GGTGATCACG CAGATCACCG CGGTGACTAA AATTTTGCAA 
TGGGGTATGA GGCAAACTGC CGAGTTAGAA AATCACATGA CTTCCATCGA GAGAGTTTTA 
GAGTACAGTC ATTTAGAGGA AGAACCCTTC TTAGACAGTA TACCGGAAAA GAAACCTCCC 
AAGGAGTGGC CCACTGCGGG CATGGTGGAA TTCAAAAATG TCAGATTAAA GTATGGACCG 
AAGAGTGCTT ACGTGTTGAA GGGTATAAAC TTCGTGGTGA AATCCAAAGA GAAAATTGGT 
GTCGTTGGGA GAACCGGTGC AGGGAAGACC TCATTGATCA GTGCTCTCTT TCGATTGGCA 
TACATAGAAG GAGAAATTAG CATAGACAAT ATACCCACAG ACACAGTTGC GTTGCACGAC 
TTCCGTTCAA AGATCAGTAT TATACCCCAA GAACCGGTGC TGTTTAGTGG TAGTCTTCGA 
AGAAATTTGG ATCCCTTTGA TGAGTATTCT GATAACGATT TATGGAGTGC GTTGCAAGAA 
GTAGAATTGA AGGAGACTAT CGCGGATATG CCAGCAGGTT TAACTAGCAA AGTAGCCGAG 
GAGGGATCAA ATTTTAGCGT TGGACAACGT CAATTATTGT GTCTTGTTAG AGCACTTGTT 
AGGAATAATA AGATTATGGT TCTCGATGAA GCCACTGCCA ATGTTGACTC GCAAACTGAT 
TCCATGATTC AAAAGACTGT TAGGAAGAAG TTTAAGCATT GTACTGTCTT CACTATAGCG 
CATAGATTAA ATACCATTAT GGATAGTGAT AAAATACTTG TGATGGACCA AGGTAACTTA 
ATGGAATTTG ATCATCCTTA TATTCTATTG CAAAAGAAAG GATATTTTTA CAATATGGTA 
CAACAAACAG GTCCTACAAT GGCAAATAAC TTATTTGAAA CAGCAAAGAA GTTTTTTTAC 
AAGAACAACG AAGCACTGTC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100877177 ( XM_012291339.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012146729.1 Megachile rotundata probable multidrug resistance-associated protein lethal(2)03659 (LOC100877177), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMe109917
Clone ID Related Accession (Same CDS sequence)	XM_003705819.2 , XM_012291340.1 , XM_012291338.1 , XM_012291339.1
Accession Version	XM_012291339.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	probable multidrug resistance-associated protein lethal(2)03659 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797352.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAA GTGAAAGGAA CCTATTGAAG AATCCGCGTC AAAATGCCAA TCTATTATCT 
ATTCTTACGT TCTGGTGGAT CCTCAAGTTG TTCATTGTCG GTTACAAAAA AGAATTGGAA 
GAAGAGGATT TGTATTGTCC ATTGAGAGAG GACAGGAGCA GTTACTTAGG CGAACGTATT 
GTAAAATATT GGGAGATTGA AGTGAAACGA CACGAGAAGA AGAAGGATAG CCCTAAACCA 
AGTTTATTTA GGGTATTGTA CAAATGCTTC GGTAAAATTG TCATGAACAC TGGACTAGCA 
CTGTTTGTTC TAGAATTCGG CATACGGATC ATACATCCTT TCCTTCTCGC TAGATTACTA 
CGTTACTTCG CTGGGAATAG AAAGGATTGG ACCAGTGATG TACATTACTA TGCAGCAGCA 
TTTTGTTTAC TACCTTTGCT AGATGTTCTG ATTATGCATT GGTCCTTACA AACCTTGACG 
CATGTCGGAA TGAAAATTAG AGTGGCTTGT TGTACCTTGA TTTATAGGAA GATATTGAGA 
CTATCCAATT CTGTCCTTGA AAATGAAACC TCTGCTGGGC AGATGGTTAA TTTCTTGAGT 
AACGACGTTA ATCGTCTCGA TTACTTCGTC TTTAGTATTC ATTATTTATG GATTGGACCT 
CTACAAGTAT TTCTAATAGC CTATTTAATA TATCGTGAGA TAGGCCTGGG AGCTATTACT 
GGGATGATGA CATTTTTATT ATGCATACCA TTACAGTTAT ATTTTGGAAA GAGAGTGTCA 
CGTTTGACTC TTGTATCGGC CCAGAAGACT GACAATAGAT TAAGTTTGAT GAACCAAATT 
ATAGCTGGTG TAGAATTAAT CAAGATGTAC GTTTGGGAGG TACCGTACTC TCTTCTCGTT 
GAAAAAGCTA GACGAAAAGA AGTGGATGTG ATCAAGAAAT ATTCGATTGT CGAGCAAATA 
GGTTTAACGT GTGATATCTA TGTTCCTCGA GTCAGCTTAT TTATTACGAT ATTAACCTAC 
GTTTTAACTG GCAACACCAT AGATGCCGAG AAAGTATTTA TGACTACTGC GTTTTACGCC 
ATTTTACAAA GCTCTATGAC CGTCGGTTTC ACTCTAAGTG TCCATCTGCT AGCTGAAGCC 
ATGGTGTCCG TCAGGCGTCT CGAGAAGTTC ATGATGCACC CCGAAATCAT TCCTCCGCCG 
AAGACGCAGA ATCAAGTAGC CACCCAATCA CTGCCCGTAT ACCTGAAAAA CGTGTCCGCC 
AGGTGGGACG AGTCCAGAGA CTGCACTTTA CAAAACGTAG ATTTATCGAT ACAAGCCGGA 
AGCTTCGTAG CGGTGATCGG ACAAATCGGT TCTGGAAAGA GTAGCCTCCT AAAAGCGATA 
CTCAGTGAGT TACCATTACA AGATGGCATC CTAGAAACCA GCGGTAAAAT AAGTTTCGCC 
GATCAACGAC CTTGGATATT TGCCTCCTCC ATCCGACAGA ACATACTGTT TGGTCAGCCT 
TACAACGAAA AGCGTTACAA CGAGGTGATT AAAGTCTGTC AACTAAAACG CGACATAGAT 
TCGTTTACGC ACAAAGACAG CACGATGGTC GGCGAAAGAG GGATCAATTT AAGTGGCGGT 
CAACGAGCTA GAATTAATTT GGCCCGTGCC ATCTACGCCG AGGCCGACAT ATATCTGTTG 
GATGATCCTC TATCGGCGGT CGACGGACAC GTGGGTAGCC ACATCGTGGA CGAGTGTATC 
TGCGGTTTCC TCAAGGGCAA GACTCGAATT CTAGTCACCC ATCAGATCCA GTACTTGAAA 
CCCGCCGATC AGATCGTCGT GATCAACGGC GGTGCCGTGC AAGCGAAGGG CAGCTTCGAG 
GAGCTTCAGA GGATGAGTCT GGACTTCATG AAGATCTTCA AAGAGGTGGA AGAGAGCAAG 
ACCAAAGAAC CCGAAACCGA GGAGAAGCAA ACGATCGAGG AGACGAAGAA AGAGGAAGAA 
CCGGAAGTAG GCAGTGAACC TGTCGAGGTG GCAGAAACGA GAACCGTAGG GAAAATTTCC 
GCCGCCGTTT TCTTAGCCTA CTGGAAAGCA AGTAAAAACC CGTGTCTGCT CGCTTTCATG 
GTGGTTCTGT TTATTTTGAG TCAAGTAATG GCGAGCGGCG CTGACTACTT GCTCGCGTTC 
TGGGTGAACA CCGAGGTAGC CTCGTGGGTT AATATCGGAA ATGGTACGAT GGCTTTTCAA 
TGGAATACTT TACTATCTCG CGATGGAATT ATTTATATTT ACAGCGCCTT AACGTTCGGC 
TTCGTCTGCG TCTATATCGT CGAAGCGTTC ACGTACTATG GGGTCTGCAT GAGAGCCTCG 
AAAAATTTGC ACGCGGCGAT GTTTCGTAGT ATTGTTCGGG CTACCATGTA CTTTTTCAAC 
ACTAATCCTG CTGGTCGTAT ATTAAACAGG TTTTCCAAAG ACATTGGTGC TATCGATAAG 
AAGCTTCCTT TCACGACGTT CGATGTCACA ATTATGTTCT TAACGTTTAT CGGAACTGTA 
GTAATTGTGG GTACTGTCAG CGCGTGGTTA TTGATACCGA CATTTGTCGC TCTACTCATT 
TTCTACTACA TGCGAGTTAT TTATATTGCA ACTAGTCGTT CTGTTAAACG TATGGAGGGT 
ACAACACGAT CGCCAGTGTT TGATCACGTC GGTGCAACTC TGCAGGGTCT CACGACAATC 
AGAGCGTTCA AAGCTGAAGA AATAGTCACA AAAGATTTCG ACAATCATCA GGACCTTCAC 
ACGTCCACCT GGTTCATATT TATAACCATT TCCCGAGCCT TCGGTCTGTA TATAGAGTGG 
TTCTGTCTGA TCTATGTAGG AATAATCACA ATCGCGTTCT TATTATTCGA GGATCTCGCT 
ATTGCCGGAG ACATAGGTTT GGTGATCACG CAGATCACCG CGGTGACTAA AATTTTGCAA 
TGGGGTATGA GGCAAACTGC CGAGTTAGAA AATCACATGA CTTCCATCGA GAGAGTTTTA 
GAGTACAGTC ATTTAGAGGA AGAACCCTTC TTAGACAGTA TACCGGAAAA GAAACCTCCC 
AAGGAGTGGC CCACTGCGGG CATGGTGGAA TTCAAAAATG TCAGATTAAA GTATGGACCG 
AAGAGTGCTT ACGTGTTGAA GGGTATAAAC TTCGTGGTGA AATCCAAAGA GAAAATTGGT 
GTCGTTGGGA GAACCGGTGC AGGGAAGACC TCATTGATCA GTGCTCTCTT TCGATTGGCA 
TACATAGAAG GAGAAATTAG CATAGACAAT ATACCCACAG ACACAGTTGC GTTGCACGAC 
TTCCGTTCAA AGATCAGTAT TATACCCCAA GAACCGGTGC TGTTTAGTGG TAGTCTTCGA 
AGAAATTTGG ATCCCTTTGA TGAGTATTCT GATAACGATT TATGGAGTGC GTTGCAAGAA 
GTAGAATTGA AGGAGACTAT CGCGGATATG CCAGCAGGTT TAACTAGCAA AGTAGCCGAG 
GAGGGATCAA ATTTTAGCGT TGGACAACGT CAATTATTGT GTCTTGTTAG AGCACTTGTT 
AGGAATAATA AGATTATGGT TCTCGATGAA GCCACTGCCA ATGTTGACTC GCAAACTGAT 
TCCATGATTC AAAAGACTGT TAGGAAGAAG TTTAAGCATT GTACTGTCTT CACTATAGCG 
CATAGATTAA ATACCATTAT GGATAGTGAT AAAATACTTG TGATGGACCA AGGTAACTTA 
ATGGAATTTG ATCATCCTTA TATTCTATTG CAAAAGAAAG GATATTTTTA CAATATGGTA 
CAACAAACAG GTCCTACAAT GGCAAATAAC TTATTTGAAA CAGCAAAGAA GTTTTTTTAC 
AAGAACAACG AAGCACTGTC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012146729.1
CDS	262..4125
Translation	MDKSERNLLKNPRQNANLLSILTFWWILKLFIVGYKKELEEEDLYCPLREDRSSYLGERIVKYWEIEVKRHEKKKDSPKPSLFRVLYKCFGKIVMNTGLALFVLEFGIRIIHPFLLARLLRYFAGNRKDWTSDVHYYAAAFCLLPLLDVLIMHWSLQTLTHVGMKIRVACCTLIYRKILRLSNSVLENETSAGQMVNFLSNDVNRLDYFVFSIHYLWIGPLQVFLIAYLIYREIGLGAITGMMTFLLCIPLQLYFGKRVSRLTLVSAQKTDNRLSLMNQIIAGVELIKMYVWEVPYSLLVEKARRKEVDVIKKYSIVEQIGLTCDIYVPRVSLFITILTYVLTGNTIDAEKVFMTTAFYAILQSSMTVGFTLSVHLLAEAMVSVRRLEKFMMHPEIIPPPKTQNQVATQSLPVYLKNVSARWDESRDCTLQNVDLSIQAGSFVAVIGQIGSGKSSLLKAILSELPLQDGILETSGKISFADQRPWIFASSIRQNILFGQPYNEKRYNEVIKVCQLKRDIDSFTHKDSTMVGERGINLSGGQRARINLARAIYAEADIYLLDDPLSAVDGHVGSHIVDECICGFLKGKTRILVTHQIQYLKPADQIVVINGGAVQAKGSFEELQRMSLDFMKIFKEVEESKTKEPETEEKQTIEETKKEEEPEVGSEPVEVAETRTVGKISAAVFLAYWKASKNPCLLAFMVVLFILSQVMASGADYLLAFWVNTEVASWVNIGNGTMAFQWNTLLSRDGIIYIYSALTFGFVCVYIVEAFTYYGVCMRASKNLHAAMFRSIVRATMYFFNTNPAGRILNRFSKDIGAIDKKLPFTTFDVTIMFLTFIGTVVIVGTVSAWLLIPTFVALLIFYYMRVIYIATSRSVKRMEGTTRSPVFDHVGATLQGLTTIRAFKAEEIVTKDFDNHQDLHTSTWFIFITISRAFGLYIEWFCLIYVGIITIAFLLFEDLAIAGDIGLVITQITAVTKILQWGMRQTAELENHMTSIERVLEYSHLEEEPFLDSIPEKKPPKEWPTAGMVEFKNVRLKYGPKSAYVLKGINFVVKSKEKIGVVGRTGAGKTSLISALFRLAYIEGEISIDNIPTDTVALHDFRSKISIIPQEPVLFSGSLRRNLDPFDEYSDNDLWSALQEVELKETIADMPAGLTSKVAEEGSNFSVGQRQLLCLVRALVRNNKIMVLDEATANVDSQTDSMIQKTVRKKFKHCTVFTIAHRLNTIMDSDKILVMDQGNLMEFDHPYILLQKKGYFYNMVQQTGPTMANNLFETAKKFFYKNNEALS

Target ORF information:

RefSeq Version	XM_012291339.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata probable multidrug resistance-associated protein lethal(2)03659 (LOC100877177), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012291339.1

ORF Insert Sequence:

ATGGATAAAA GTGAAAGGAA CCTATTGAAG AATCCGCGTC AAAATGCCAA TCTATTATCT 
ATTCTTACGT TCTGGTGGAT CCTCAAGTTG TTCATTGTCG GTTACAAAAA AGAATTGGAA 
GAAGAGGATT TGTATTGTCC ATTGAGAGAG GACAGGAGCA GTTACTTAGG CGAACGTATT 
GTAAAATATT GGGAGATTGA AGTGAAACGA CACGAGAAGA AGAAGGATAG CCCTAAACCA 
AGTTTATTTA GGGTATTGTA CAAATGCTTC GGTAAAATTG TCATGAACAC TGGACTAGCA 
CTGTTTGTTC TAGAATTCGG CATACGGATC ATACATCCTT TCCTTCTCGC TAGATTACTA 
CGTTACTTCG CTGGGAATAG AAAGGATTGG ACCAGTGATG TACATTACTA TGCAGCAGCA 
TTTTGTTTAC TACCTTTGCT AGATGTTCTG ATTATGCATT GGTCCTTACA AACCTTGACG 
CATGTCGGAA TGAAAATTAG AGTGGCTTGT TGTACCTTGA TTTATAGGAA GATATTGAGA 
CTATCCAATT CTGTCCTTGA AAATGAAACC TCTGCTGGGC AGATGGTTAA TTTCTTGAGT 
AACGACGTTA ATCGTCTCGA TTACTTCGTC TTTAGTATTC ATTATTTATG GATTGGACCT 
CTACAAGTAT TTCTAATAGC CTATTTAATA TATCGTGAGA TAGGCCTGGG AGCTATTACT 
GGGATGATGA CATTTTTATT ATGCATACCA TTACAGTTAT ATTTTGGAAA GAGAGTGTCA 
CGTTTGACTC TTGTATCGGC CCAGAAGACT GACAATAGAT TAAGTTTGAT GAACCAAATT 
ATAGCTGGTG TAGAATTAAT CAAGATGTAC GTTTGGGAGG TACCGTACTC TCTTCTCGTT 
GAAAAAGCTA GACGAAAAGA AGTGGATGTG ATCAAGAAAT ATTCGATTGT CGAGCAAATA 
GGTTTAACGT GTGATATCTA TGTTCCTCGA GTCAGCTTAT TTATTACGAT ATTAACCTAC 
GTTTTAACTG GCAACACCAT AGATGCCGAG AAAGTATTTA TGACTACTGC GTTTTACGCC 
ATTTTACAAA GCTCTATGAC CGTCGGTTTC ACTCTAAGTG TCCATCTGCT AGCTGAAGCC 
ATGGTGTCCG TCAGGCGTCT CGAGAAGTTC ATGATGCACC CCGAAATCAT TCCTCCGCCG 
AAGACGCAGA ATCAAGTAGC CACCCAATCA CTGCCCGTAT ACCTGAAAAA CGTGTCCGCC 
AGGTGGGACG AGTCCAGAGA CTGCACTTTA CAAAACGTAG ATTTATCGAT ACAAGCCGGA 
AGCTTCGTAG CGGTGATCGG ACAAATCGGT TCTGGAAAGA GTAGCCTCCT AAAAGCGATA 
CTCAGTGAGT TACCATTACA AGATGGCATC CTAGAAACCA GCGGTAAAAT AAGTTTCGCC 
GATCAACGAC CTTGGATATT TGCCTCCTCC ATCCGACAGA ACATACTGTT TGGTCAGCCT 
TACAACGAAA AGCGTTACAA CGAGGTGATT AAAGTCTGTC AACTAAAACG CGACATAGAT 
TCGTTTACGC ACAAAGACAG CACGATGGTC GGCGAAAGAG GGATCAATTT AAGTGGCGGT 
CAACGAGCTA GAATTAATTT GGCCCGTGCC ATCTACGCCG AGGCCGACAT ATATCTGTTG 
GATGATCCTC TATCGGCGGT CGACGGACAC GTGGGTAGCC ACATCGTGGA CGAGTGTATC 
TGCGGTTTCC TCAAGGGCAA GACTCGAATT CTAGTCACCC ATCAGATCCA GTACTTGAAA 
CCCGCCGATC AGATCGTCGT GATCAACGGC GGTGCCGTGC AAGCGAAGGG CAGCTTCGAG 
GAGCTTCAGA GGATGAGTCT GGACTTCATG AAGATCTTCA AAGAGGTGGA AGAGAGCAAG 
ACCAAAGAAC CCGAAACCGA GGAGAAGCAA ACGATCGAGG AGACGAAGAA AGAGGAAGAA 
CCGGAAGTAG GCAGTGAACC TGTCGAGGTG GCAGAAACGA GAACCGTAGG GAAAATTTCC 
GCCGCCGTTT TCTTAGCCTA CTGGAAAGCA AGTAAAAACC CGTGTCTGCT CGCTTTCATG 
GTGGTTCTGT TTATTTTGAG TCAAGTAATG GCGAGCGGCG CTGACTACTT GCTCGCGTTC 
TGGGTGAACA CCGAGGTAGC CTCGTGGGTT AATATCGGAA ATGGTACGAT GGCTTTTCAA 
TGGAATACTT TACTATCTCG CGATGGAATT ATTTATATTT ACAGCGCCTT AACGTTCGGC 
TTCGTCTGCG TCTATATCGT CGAAGCGTTC ACGTACTATG GGGTCTGCAT GAGAGCCTCG 
AAAAATTTGC ACGCGGCGAT GTTTCGTAGT ATTGTTCGGG CTACCATGTA CTTTTTCAAC 
ACTAATCCTG CTGGTCGTAT ATTAAACAGG TTTTCCAAAG ACATTGGTGC TATCGATAAG 
AAGCTTCCTT TCACGACGTT CGATGTCACA ATTATGTTCT TAACGTTTAT CGGAACTGTA 
GTAATTGTGG GTACTGTCAG CGCGTGGTTA TTGATACCGA CATTTGTCGC TCTACTCATT 
TTCTACTACA TGCGAGTTAT TTATATTGCA ACTAGTCGTT CTGTTAAACG TATGGAGGGT 
ACAACACGAT CGCCAGTGTT TGATCACGTC GGTGCAACTC TGCAGGGTCT CACGACAATC 
AGAGCGTTCA AAGCTGAAGA AATAGTCACA AAAGATTTCG ACAATCATCA GGACCTTCAC 
ACGTCCACCT GGTTCATATT TATAACCATT TCCCGAGCCT TCGGTCTGTA TATAGAGTGG 
TTCTGTCTGA TCTATGTAGG AATAATCACA ATCGCGTTCT TATTATTCGA GGATCTCGCT 
ATTGCCGGAG ACATAGGTTT GGTGATCACG CAGATCACCG CGGTGACTAA AATTTTGCAA 
TGGGGTATGA GGCAAACTGC CGAGTTAGAA AATCACATGA CTTCCATCGA GAGAGTTTTA 
GAGTACAGTC ATTTAGAGGA AGAACCCTTC TTAGACAGTA TACCGGAAAA GAAACCTCCC 
AAGGAGTGGC CCACTGCGGG CATGGTGGAA TTCAAAAATG TCAGATTAAA GTATGGACCG 
AAGAGTGCTT ACGTGTTGAA GGGTATAAAC TTCGTGGTGA AATCCAAAGA GAAAATTGGT 
GTCGTTGGGA GAACCGGTGC AGGGAAGACC TCATTGATCA GTGCTCTCTT TCGATTGGCA 
TACATAGAAG GAGAAATTAG CATAGACAAT ATACCCACAG ACACAGTTGC GTTGCACGAC 
TTCCGTTCAA AGATCAGTAT TATACCCCAA GAACCGGTGC TGTTTAGTGG TAGTCTTCGA 
AGAAATTTGG ATCCCTTTGA TGAGTATTCT GATAACGATT TATGGAGTGC GTTGCAAGAA 
GTAGAATTGA AGGAGACTAT CGCGGATATG CCAGCAGGTT TAACTAGCAA AGTAGCCGAG 
GAGGGATCAA ATTTTAGCGT TGGACAACGT CAATTATTGT GTCTTGTTAG AGCACTTGTT 
AGGAATAATA AGATTATGGT TCTCGATGAA GCCACTGCCA ATGTTGACTC GCAAACTGAT 
TCCATGATTC AAAAGACTGT TAGGAAGAAG TTTAAGCATT GTACTGTCTT CACTATAGCG 
CATAGATTAA ATACCATTAT GGATAGTGAT AAAATACTTG TGATGGACCA AGGTAACTTA 
ATGGAATTTG ATCATCCTTA TATTCTATTG CAAAAGAAAG GATATTTTTA CAATATGGTA 
CAACAAACAG GTCCTACAAT GGCAAATAAC TTATTTGAAA CAGCAAAGAA GTTTTTTTAC 
AAGAACAACG AAGCACTGTC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100877177 ( XM_012291341.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012146731.1 Megachile rotundata probable multidrug resistance-associated protein lethal(2)03659 (LOC100877177), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMe109918
Clone ID Related Accession (Same CDS sequence)	XM_012291341.1
Accession Version	XM_012291341.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	probable multidrug resistance-associated protein lethal(2)03659 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797352.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACACTG GACTAGCACT GTTTGTTCTA GAATTCGGCA TACGGATCAT ACATCCTTTC 
CTTCTCGCTA GATTACTACG TTACTTCGCT GGGAATAGAA AGGATTGGAC CAGTGATGTA 
CATTACTATG CAGCAGCATT TTGTTTACTA CCTTTGCTAG ATGTTCTGAT TATGCATTGG 
TCCTTACAAA CCTTGACGCA TGTCGGAATG AAAATTAGAG TGGCTTGTTG TACCTTGATT 
TATAGGAAGA TATTGAGACT ATCCAATTCT GTCCTTGAAA ATGAAACCTC TGCTGGGCAG 
ATGGTTAATT TCTTGAGTAA CGACGTTAAT CGTCTCGATT ACTTCGTCTT TAGTATTCAT 
TATTTATGGA TTGGACCTCT ACAAGTATTT CTAATAGCCT ATTTAATATA TCGTGAGATA 
GGCCTGGGAG CTATTACTGG GATGATGACA TTTTTATTAT GCATACCATT ACAGTTATAT 
TTTGGAAAGA GAGTGTCACG TTTGACTCTT GTATCGGCCC AGAAGACTGA CAATAGATTA 
AGTTTGATGA ACCAAATTAT AGCTGGTGTA GAATTAATCA AGATGTACGT TTGGGAGGTA 
CCGTACTCTC TTCTCGTTGA AAAAGCTAGA CGAAAAGAAG TGGATGTGAT CAAGAAATAT 
TCGATTGTCG AGCAAATAGG TTTAACGTGT GATATCTATG TTCCTCGAGT CAGCTTATTT 
ATTACGATAT TAACCTACGT TTTAACTGGC AACACCATAG ATGCCGAGAA AGTATTTATG 
ACTACTGCGT TTTACGCCAT TTTACAAAGC TCTATGACCG TCGGTTTCAC TCTAAGTGTC 
CATCTGCTAG CTGAAGCCAT GGTGTCCGTC AGGCGTCTCG AGAAGTTCAT GATGCACCCC 
GAAATCATTC CTCCGCCGAA GACGCAGAAT CAAGTAGCCA CCCAATCACT GCCCGTATAC 
CTGAAAAACG TGTCCGCCAG GTGGGACGAG TCCAGAGACT GCACTTTACA AAACGTAGAT 
TTATCGATAC AAGCCGGAAG CTTCGTAGCG GTGATCGGAC AAATCGGTTC TGGAAAGAGT 
AGCCTCCTAA AAGCGATACT CAGTGAGTTA CCATTACAAG ATGGCATCCT AGAAACCAGC 
GGTAAAATAA GTTTCGCCGA TCAACGACCT TGGATATTTG CCTCCTCCAT CCGACAGAAC 
ATACTGTTTG GTCAGCCTTA CAACGAAAAG CGTTACAACG AGGTGATTAA AGTCTGTCAA 
CTAAAACGCG ACATAGATTC GTTTACGCAC AAAGACAGCA CGATGGTCGG CGAAAGAGGG 
ATCAATTTAA GTGGCGGTCA ACGAGCTAGA ATTAATTTGG CCCGTGCCAT CTACGCCGAG 
GCCGACATAT ATCTGTTGGA TGATCCTCTA TCGGCGGTCG ACGGACACGT GGGTAGCCAC 
ATCGTGGACG AGTGTATCTG CGGTTTCCTC AAGGGCAAGA CTCGAATTCT AGTCACCCAT 
CAGATCCAGT ACTTGAAACC CGCCGATCAG ATCGTCGTGA TCAACGGCGG TGCCGTGCAA 
GCGAAGGGCA GCTTCGAGGA GCTTCAGAGG ATGAGTCTGG ACTTCATGAA GATCTTCAAA 
GAGGTGGAAG AGAGCAAGAC CAAAGAACCC GAAACCGAGG AGAAGCAAAC GATCGAGGAG 
ACGAAGAAAG AGGAAGAACC GGAAGTAGGC AGTGAACCTG TCGAGGTGGC AGAAACGAGA 
ACCGTAGGGA AAATTTCCGC CGCCGTTTTC TTAGCCTACT GGAAAGCAAG TAAAAACCCG 
TGTCTGCTCG CTTTCATGGT GGTTCTGTTT ATTTTGAGTC AAGTAATGGC GAGCGGCGCT 
GACTACTTGC TCGCGTTCTG GGTGAACACC GAGGTAGCCT CGTGGGTTAA TATCGGAAAT 
GGTACGATGG CTTTTCAATG GAATACTTTA CTATCTCGCG ATGGAATTAT TTATATTTAC 
AGCGCCTTAA CGTTCGGCTT CGTCTGCGTC TATATCGTCG AAGCGTTCAC GTACTATGGG 
GTCTGCATGA GAGCCTCGAA AAATTTGCAC GCGGCGATGT TTCGTAGTAT TGTTCGGGCT 
ACCATGTACT TTTTCAACAC TAATCCTGCT GGTCGTATAT TAAACAGGTT TTCCAAAGAC 
ATTGGTGCTA TCGATAAGAA GCTTCCTTTC ACGACGTTCG ATGTCACAAT TATGTTCTTA 
ACGTTTATCG GAACTGTAGT AATTGTGGGT ACTGTCAGCG CGTGGTTATT GATACCGACA 
TTTGTCGCTC TACTCATTTT CTACTACATG CGAGTTATTT ATATTGCAAC TAGTCGTTCT 
GTTAAACGTA TGGAGGGTAC AACACGATCG CCAGTGTTTG ATCACGTCGG TGCAACTCTG 
CAGGGTCTCA CGACAATCAG AGCGTTCAAA GCTGAAGAAA TAGTCACAAA AGATTTCGAC 
AATCATCAGG ACCTTCACAC GTCCACCTGG TTCATATTTA TAACCATTTC CCGAGCCTTC 
GGTCTGTATA TAGAGTGGTT CTGTCTGATC TATGTAGGAA TAATCACAAT CGCGTTCTTA 
TTATTCGAGG ATCTCGCTAT TGCCGGAGAC ATAGGTTTGG TGATCACGCA GATCACCGCG 
GTGACTAAAA TTTTGCAATG GGGTATGAGG CAAACTGCCG AGTTAGAAAA TCACATGACT 
TCCATCGAGA GAGTTTTAGA GTACAGTCAT TTAGAGGAAG AACCCTTCTT AGACAGTATA 
CCGGAAAAGA AACCTCCCAA GGAGTGGCCC ACTGCGGGCA TGGTGGAATT CAAAAATGTC 
AGATTAAAGT ATGGACCGAA GAGTGCTTAC GTGTTGAAGG GTATAAACTT CGTGGTGAAA 
TCCAAAGAGA AAATTGGTGT CGTTGGGAGA ACCGGTGCAG GGAAGACCTC ATTGATCAGT 
GCTCTCTTTC GATTGGCATA CATAGAAGGA GAAATTAGCA TAGACAATAT ACCCACAGAC 
ACAGTTGCGT TGCACGACTT CCGTTCAAAG ATCAGTATTA TACCCCAAGA ACCGGTGCTG 
TTTAGTGGTA GTCTTCGAAG AAATTTGGAT CCCTTTGATG AGTATTCTGA TAACGATTTA 
TGGAGTGCGT TGCAAGAAGT AGAATTGAAG GAGACTATCG CGGATATGCC AGCAGGTTTA 
ACTAGCAAAG TAGCCGAGGA GGGATCAAAT TTTAGCGTTG GACAACGTCA ATTATTGTGT 
CTTGTTAGAG CACTTGTTAG GAATAATAAG ATTATGGTTC TCGATGAAGC CACTGCCAAT 
GTTGACTCGC AAACTGATTC CATGATTCAA AAGACTGTTA GGAAGAAGTT TAAGCATTGT 
ACTGTCTTCA CTATAGCGCA TAGATTAAAT ACCATTATGG ATAGTGATAA AATACTTGTG 
ATGGACCAAG GTAACTTAAT GGAATTTGAT CATCCTTATA TTCTATTGCA AAAGAAAGGA 
TATTTTTACA ATATGGTACA ACAAACAGGT CCTACAATGG CAAATAACTT ATTTGAAACA 
GCAAAGAAGT TTTTTTACAA GAACAACGAA GCACTGTCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012146731.1
CDS	226..3807
Translation	MNTGLALFVLEFGIRIIHPFLLARLLRYFAGNRKDWTSDVHYYAAAFCLLPLLDVLIMHWSLQTLTHVGMKIRVACCTLIYRKILRLSNSVLENETSAGQMVNFLSNDVNRLDYFVFSIHYLWIGPLQVFLIAYLIYREIGLGAITGMMTFLLCIPLQLYFGKRVSRLTLVSAQKTDNRLSLMNQIIAGVELIKMYVWEVPYSLLVEKARRKEVDVIKKYSIVEQIGLTCDIYVPRVSLFITILTYVLTGNTIDAEKVFMTTAFYAILQSSMTVGFTLSVHLLAEAMVSVRRLEKFMMHPEIIPPPKTQNQVATQSLPVYLKNVSARWDESRDCTLQNVDLSIQAGSFVAVIGQIGSGKSSLLKAILSELPLQDGILETSGKISFADQRPWIFASSIRQNILFGQPYNEKRYNEVIKVCQLKRDIDSFTHKDSTMVGERGINLSGGQRARINLARAIYAEADIYLLDDPLSAVDGHVGSHIVDECICGFLKGKTRILVTHQIQYLKPADQIVVINGGAVQAKGSFEELQRMSLDFMKIFKEVEESKTKEPETEEKQTIEETKKEEEPEVGSEPVEVAETRTVGKISAAVFLAYWKASKNPCLLAFMVVLFILSQVMASGADYLLAFWVNTEVASWVNIGNGTMAFQWNTLLSRDGIIYIYSALTFGFVCVYIVEAFTYYGVCMRASKNLHAAMFRSIVRATMYFFNTNPAGRILNRFSKDIGAIDKKLPFTTFDVTIMFLTFIGTVVIVGTVSAWLLIPTFVALLIFYYMRVIYIATSRSVKRMEGTTRSPVFDHVGATLQGLTTIRAFKAEEIVTKDFDNHQDLHTSTWFIFITISRAFGLYIEWFCLIYVGIITIAFLLFEDLAIAGDIGLVITQITAVTKILQWGMRQTAELENHMTSIERVLEYSHLEEEPFLDSIPEKKPPKEWPTAGMVEFKNVRLKYGPKSAYVLKGINFVVKSKEKIGVVGRTGAGKTSLISALFRLAYIEGEISIDNIPTDTVALHDFRSKISIIPQEPVLFSGSLRRNLDPFDEYSDNDLWSALQEVELKETIADMPAGLTSKVAEEGSNFSVGQRQLLCLVRALVRNNKIMVLDEATANVDSQTDSMIQKTVRKKFKHCTVFTIAHRLNTIMDSDKILVMDQGNLMEFDHPYILLQKKGYFYNMVQQTGPTMANNLFETAKKFFYKNNEALS

Target ORF information:

RefSeq Version	XM_012291341.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata probable multidrug resistance-associated protein lethal(2)03659 (LOC100877177), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012291341.1

ORF Insert Sequence:

ATGAACACTG GACTAGCACT GTTTGTTCTA GAATTCGGCA TACGGATCAT ACATCCTTTC 
CTTCTCGCTA GATTACTACG TTACTTCGCT GGGAATAGAA AGGATTGGAC CAGTGATGTA 
CATTACTATG CAGCAGCATT TTGTTTACTA CCTTTGCTAG ATGTTCTGAT TATGCATTGG 
TCCTTACAAA CCTTGACGCA TGTCGGAATG AAAATTAGAG TGGCTTGTTG TACCTTGATT 
TATAGGAAGA TATTGAGACT ATCCAATTCT GTCCTTGAAA ATGAAACCTC TGCTGGGCAG 
ATGGTTAATT TCTTGAGTAA CGACGTTAAT CGTCTCGATT ACTTCGTCTT TAGTATTCAT 
TATTTATGGA TTGGACCTCT ACAAGTATTT CTAATAGCCT ATTTAATATA TCGTGAGATA 
GGCCTGGGAG CTATTACTGG GATGATGACA TTTTTATTAT GCATACCATT ACAGTTATAT 
TTTGGAAAGA GAGTGTCACG TTTGACTCTT GTATCGGCCC AGAAGACTGA CAATAGATTA 
AGTTTGATGA ACCAAATTAT AGCTGGTGTA GAATTAATCA AGATGTACGT TTGGGAGGTA 
CCGTACTCTC TTCTCGTTGA AAAAGCTAGA CGAAAAGAAG TGGATGTGAT CAAGAAATAT 
TCGATTGTCG AGCAAATAGG TTTAACGTGT GATATCTATG TTCCTCGAGT CAGCTTATTT 
ATTACGATAT TAACCTACGT TTTAACTGGC AACACCATAG ATGCCGAGAA AGTATTTATG 
ACTACTGCGT TTTACGCCAT TTTACAAAGC TCTATGACCG TCGGTTTCAC TCTAAGTGTC 
CATCTGCTAG CTGAAGCCAT GGTGTCCGTC AGGCGTCTCG AGAAGTTCAT GATGCACCCC 
GAAATCATTC CTCCGCCGAA GACGCAGAAT CAAGTAGCCA CCCAATCACT GCCCGTATAC 
CTGAAAAACG TGTCCGCCAG GTGGGACGAG TCCAGAGACT GCACTTTACA AAACGTAGAT 
TTATCGATAC AAGCCGGAAG CTTCGTAGCG GTGATCGGAC AAATCGGTTC TGGAAAGAGT 
AGCCTCCTAA AAGCGATACT CAGTGAGTTA CCATTACAAG ATGGCATCCT AGAAACCAGC 
GGTAAAATAA GTTTCGCCGA TCAACGACCT TGGATATTTG CCTCCTCCAT CCGACAGAAC 
ATACTGTTTG GTCAGCCTTA CAACGAAAAG CGTTACAACG AGGTGATTAA AGTCTGTCAA 
CTAAAACGCG ACATAGATTC GTTTACGCAC AAAGACAGCA CGATGGTCGG CGAAAGAGGG 
ATCAATTTAA GTGGCGGTCA ACGAGCTAGA ATTAATTTGG CCCGTGCCAT CTACGCCGAG 
GCCGACATAT ATCTGTTGGA TGATCCTCTA TCGGCGGTCG ACGGACACGT GGGTAGCCAC 
ATCGTGGACG AGTGTATCTG CGGTTTCCTC AAGGGCAAGA CTCGAATTCT AGTCACCCAT 
CAGATCCAGT ACTTGAAACC CGCCGATCAG ATCGTCGTGA TCAACGGCGG TGCCGTGCAA 
GCGAAGGGCA GCTTCGAGGA GCTTCAGAGG ATGAGTCTGG ACTTCATGAA GATCTTCAAA 
GAGGTGGAAG AGAGCAAGAC CAAAGAACCC GAAACCGAGG AGAAGCAAAC GATCGAGGAG 
ACGAAGAAAG AGGAAGAACC GGAAGTAGGC AGTGAACCTG TCGAGGTGGC AGAAACGAGA 
ACCGTAGGGA AAATTTCCGC CGCCGTTTTC TTAGCCTACT GGAAAGCAAG TAAAAACCCG 
TGTCTGCTCG CTTTCATGGT GGTTCTGTTT ATTTTGAGTC AAGTAATGGC GAGCGGCGCT 
GACTACTTGC TCGCGTTCTG GGTGAACACC GAGGTAGCCT CGTGGGTTAA TATCGGAAAT 
GGTACGATGG CTTTTCAATG GAATACTTTA CTATCTCGCG ATGGAATTAT TTATATTTAC 
AGCGCCTTAA CGTTCGGCTT CGTCTGCGTC TATATCGTCG AAGCGTTCAC GTACTATGGG 
GTCTGCATGA GAGCCTCGAA AAATTTGCAC GCGGCGATGT TTCGTAGTAT TGTTCGGGCT 
ACCATGTACT TTTTCAACAC TAATCCTGCT GGTCGTATAT TAAACAGGTT TTCCAAAGAC 
ATTGGTGCTA TCGATAAGAA GCTTCCTTTC ACGACGTTCG ATGTCACAAT TATGTTCTTA 
ACGTTTATCG GAACTGTAGT AATTGTGGGT ACTGTCAGCG CGTGGTTATT GATACCGACA 
TTTGTCGCTC TACTCATTTT CTACTACATG CGAGTTATTT ATATTGCAAC TAGTCGTTCT 
GTTAAACGTA TGGAGGGTAC AACACGATCG CCAGTGTTTG ATCACGTCGG TGCAACTCTG 
CAGGGTCTCA CGACAATCAG AGCGTTCAAA GCTGAAGAAA TAGTCACAAA AGATTTCGAC 
AATCATCAGG ACCTTCACAC GTCCACCTGG TTCATATTTA TAACCATTTC CCGAGCCTTC 
GGTCTGTATA TAGAGTGGTT CTGTCTGATC TATGTAGGAA TAATCACAAT CGCGTTCTTA 
TTATTCGAGG ATCTCGCTAT TGCCGGAGAC ATAGGTTTGG TGATCACGCA GATCACCGCG 
GTGACTAAAA TTTTGCAATG GGGTATGAGG CAAACTGCCG AGTTAGAAAA TCACATGACT 
TCCATCGAGA GAGTTTTAGA GTACAGTCAT TTAGAGGAAG AACCCTTCTT AGACAGTATA 
CCGGAAAAGA AACCTCCCAA GGAGTGGCCC ACTGCGGGCA TGGTGGAATT CAAAAATGTC 
AGATTAAAGT ATGGACCGAA GAGTGCTTAC GTGTTGAAGG GTATAAACTT CGTGGTGAAA 
TCCAAAGAGA AAATTGGTGT CGTTGGGAGA ACCGGTGCAG GGAAGACCTC ATTGATCAGT 
GCTCTCTTTC GATTGGCATA CATAGAAGGA GAAATTAGCA TAGACAATAT ACCCACAGAC 
ACAGTTGCGT TGCACGACTT CCGTTCAAAG ATCAGTATTA TACCCCAAGA ACCGGTGCTG 
TTTAGTGGTA GTCTTCGAAG AAATTTGGAT CCCTTTGATG AGTATTCTGA TAACGATTTA 
TGGAGTGCGT TGCAAGAAGT AGAATTGAAG GAGACTATCG CGGATATGCC AGCAGGTTTA 
ACTAGCAAAG TAGCCGAGGA GGGATCAAAT TTTAGCGTTG GACAACGTCA ATTATTGTGT 
CTTGTTAGAG CACTTGTTAG GAATAATAAG ATTATGGTTC TCGATGAAGC CACTGCCAAT 
GTTGACTCGC AAACTGATTC CATGATTCAA AAGACTGTTA GGAAGAAGTT TAAGCATTGT 
ACTGTCTTCA CTATAGCGCA TAGATTAAAT ACCATTATGG ATAGTGATAA AATACTTGTG 
ATGGACCAAG GTAACTTAAT GGAATTTGAT CATCCTTATA TTCTATTGCA AAAGAAAGGA 
TATTTTTACA ATATGGTACA ACAAACAGGT CCTACAATGG CAAATAACTT ATTTGAAACA 
GCAAAGAAGT TTTTTTACAA GAACAACGAA GCACTGTCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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電話

03-6811-6572

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03-6811-6573
050-3174-7589(受注専用)

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オンライン注文

LOC100877177 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム