LOC100877150 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100877150
Entrez Gene ID	100877150
Full Name	glutaminyl-peptide cyclotransferase-like
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003708454.2	XP_003708502.2	glutaminyl-peptide cyclotransferase-like isoform X1
XM_012297754.1	XP_012153144.1	glutaminyl-peptide cyclotransferase-like isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC100877150 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100877150 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe115544	XM_003708454.2 Latest version!	Megachile rotundata glutaminyl-peptide cyclotransferase-like (LOC100877150), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OMe115545	XM_012297754.1 Latest version!	Megachile rotundata glutaminyl-peptide cyclotransferase-like (LOC100877150), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100877150 ( XM_003708454.2 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_003708502.2 Megachile rotundata glutaminyl-peptide cyclotransferase-like (LOC100877150), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMe115544
Clone ID Related Accession (Same CDS sequence)	XM_003708454.2
Accession Version	XM_003708454.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	glutaminyl-peptide cyclotransferase-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003799051.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2015 this sequence version replaced XM_003708454.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTTCG ATTTTCTTTT ACAGATTTTT TTCATTTTCG TCGGGCCTTT CGTAACCGAC 
GCAAGTATTT CGACAACAAT GCGTTTTCAA AATCAAAAGT ACTATCATAT GCCTATTACT 
CTATCGAGAA GTCAGATACT GGAAATATCG GATTTGACAA ATGTCAATCA TATGGGCCAA 
ATTTTAGATA ACATATGTGT AGTGAGAATA GTCGGTACTC CAGAACATAC AAAAGTAAAA 
AATTACATTA AGGAGTCAAT GAACAACCTT AATTGGACGG TAGAATCCGA TATTTTTGAA 
GAACATACGC CAACTTTTGG AATTCTAAAA TTTGAAAATA TAATTGCAAA GTTAAATCCT 
AACGCGCGTA GATATTTAGC ATTGGCTTGC CATTACGACT CCAAATATAC TAGGGAAAGA 
AATTTTATCG GCGCTACCGA TAGTGCAGTT CCCTGTGCTC AAATGATTAA TTTAGCCAAA 
GTTATGCAAA ATCGCTTAAA TTCTATAAGA CACAACGATA TTAGTTTAAT GTTTATATTC 
TTTGACGGAG AAGAAGCTTT TAAAGAATGG GGTCCAAAAG ATTCCATATA CGGAGCAAGA 
CATTTAGCCA AAATTTGGCA TGATAATTAC ACCTCTTACA ACGAAGGAGA AAATATTTCA 
GAATTAGACA AAATTGATAT ATTGGTTCTT TTAGACCTGA TCGGCGCACC GGATCCAATG 
TTTTATAATT ATTTTAGCAA TACCGAAAAG TGGTATTCTT TATTAATGGC TATTGAAAGT 
AATTTAGCAG CTTTAAAAAA ATTTGAATCG TACTCGTACG AGCAACCTAC TCAGAGATAT 
TTTCAACCAC ATTCGATGGA AGCGCATATC GAAGATGATC ATATTCCGTT TTTGCAAAGA 
GACATTCCTA TTTTACACCT TATACCGTAT CCCTTTCCAA AAGTTTGGCA CAAACCAAGC 
GATCATTGTG ATAATATAGA TATAAAAACT ACGGAAAACA TTAATAAGAT TTTGAGGATT 
TTTGTAGCGT CGTATTTACA TATCAGTATG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003708502.2
CDS	60..1112
Translation	MMFDFLLQIFFIFVGPFVTDASISTTMRFQNQKYYHMPITLSRSQILEISDLTNVNHMGQILDNICVVRIVGTPEHTKVKNYIKESMNNLNWTVESDIFEEHTPTFGILKFENIIAKLNPNARRYLALACHYDSKYTRERNFIGATDSAVPCAQMINLAKVMQNRLNSIRHNDISLMFIFFDGEEAFKEWGPKDSIYGARHLAKIWHDNYTSYNEGENISELDKIDILVLLDLIGAPDPMFYNYFSNTEKWYSLLMAIESNLAALKKFESYSYEQPTQRYFQPHSMEAHIEDDHIPFLQRDIPILHLIPYPFPKVWHKPSDHCDNIDIKTTENINKILRIFVASYLHISM

Target ORF information:

RefSeq Version	XM_003708454.2
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata glutaminyl-peptide cyclotransferase-like (LOC100877150), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003708454.2

ORF Insert Sequence:

ATGATGTTCG ATTTTCTTTT ACAGATTTTT TTCATTTTCG TCGGGCCTTT CGTAACCGAC 
GCAAGTATTT CGACAACAAT GCGTTTTCAA AATCAAAAGT ACTATCATAT GCCTATTACT 
CTATCGAGAA GTCAGATACT GGAAATATCG GATTTGACAA ATGTCAATCA TATGGGCCAA 
ATTTTAGATA ACATATGTGT AGTGAGAATA GTCGGTACTC CAGAACATAC AAAAGTAAAA 
AATTACATTA AGGAGTCAAT GAACAACCTT AATTGGACGG TAGAATCCGA TATTTTTGAA 
GAACATACGC CAACTTTTGG AATTCTAAAA TTTGAAAATA TAATTGCAAA GTTAAATCCT 
AACGCGCGTA GATATTTAGC ATTGGCTTGC CATTACGACT CCAAATATAC TAGGGAAAGA 
AATTTTATCG GCGCTACCGA TAGTGCAGTT CCCTGTGCTC AAATGATTAA TTTAGCCAAA 
GTTATGCAAA ATCGCTTAAA TTCTATAAGA CACAACGATA TTAGTTTAAT GTTTATATTC 
TTTGACGGAG AAGAAGCTTT TAAAGAATGG GGTCCAAAAG ATTCCATATA CGGAGCAAGA 
CATTTAGCCA AAATTTGGCA TGATAATTAC ACCTCTTACA ACGAAGGAGA AAATATTTCA 
GAATTAGACA AAATTGATAT ATTGGTTCTT TTAGACCTGA TCGGCGCACC GGATCCAATG 
TTTTATAATT ATTTTAGCAA TACCGAAAAG TGGTATTCTT TATTAATGGC TATTGAAAGT 
AATTTAGCAG CTTTAAAAAA ATTTGAATCG TACTCGTACG AGCAACCTAC TCAGAGATAT 
TTTCAACCAC ATTCGATGGA AGCGCATATC GAAGATGATC ATATTCCGTT TTTGCAAAGA 
GACATTCCTA TTTTACACCT TATACCGTAT CCCTTTCCAA AAGTTTGGCA CAAACCAAGC 
GATCATTGTG ATAATATAGA TATAAAAACT ACGGAAAACA TTAATAAGAT TTTGAGGATT 
TTTGTAGCGT CGTATTTACA TATCAGTATG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100877150 ( XM_012297754.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012153144.1 Megachile rotundata glutaminyl-peptide cyclotransferase-like (LOC100877150), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMe115545
Clone ID Related Accession (Same CDS sequence)	XM_012297754.1
Accession Version	XM_012297754.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	glutaminyl-peptide cyclotransferase-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003799051.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTTCG ATTTTCTTTT ACAGATTTTT TTCATTTTCG TCGGGCCTTT CGTAACCGAC 
GCAAGTATTT CGACAACAAT GCGTTTTCAA AATCAAAAGT ACTATCATAT GCCTATTACT 
CTATCGAGAA GTCAGATACT GGAAATATCG GATTTGACAA ATGTCAATCA TATGGGCCAA 
ATTTTAGATA ACATATGTGT AGTGAGAATA GTCGGTACTC CAGAACATAC AAAAGTAAAA 
AATAACGATA TTAGTTTAAT GTTTATATTC TTTGACGGAG AAGAAGCTTT TAAAGAATGG 
GGTCCAAAAG ATTCCATATA CGGAGCAAGA CATTTAGCCA AAATTTGGCA TGATAATTAC 
ACCTCTTACA ACGAAGGAGA AAATATTTCA GAATTAGACA AAATTGATAT ATTGGTTCTT 
TTAGACCTGA TCGGCGCACC GGATCCAATG TTTTATAATT ATTTTAGCAA TACCGAAAAG 
TGGTATTCTT TATTAATGGC TATTGAAAGT AATTTAGCAG CTTTAAAAAA ATTTGAATCG 
TACTCGTACG AGCAACCTAC TCAGAGATAT TTTCAACCAC ATTCGATGGA AGCGCATATC 
GAAGATGATC ATATTCCGTT TTTGCAAAGA GACATTCCTA TTTTACACCT TATACCGTAT 
CCCTTTCCAA AAGTTTGGCA CAAACCAAGC GATCATTGTG ATAATATAGA TATAAAAACT 
ACGGAAAACA TTAATAAGAT TTTGAGGATT TTTGTAGCGT CGTATTTACA TATCAGTATG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012153144.1
CDS	62..844
Translation	MMFDFLLQIFFIFVGPFVTDASISTTMRFQNQKYYHMPITLSRSQILEISDLTNVNHMGQILDNICVVRIVGTPEHTKVKNNDISLMFIFFDGEEAFKEWGPKDSIYGARHLAKIWHDNYTSYNEGENISELDKIDILVLLDLIGAPDPMFYNYFSNTEKWYSLLMAIESNLAALKKFESYSYEQPTQRYFQPHSMEAHIEDDHIPFLQRDIPILHLIPYPFPKVWHKPSDHCDNIDIKTTENINKILRIFVASYLHISM

Target ORF information:

RefSeq Version	XM_012297754.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata glutaminyl-peptide cyclotransferase-like (LOC100877150), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012297754.1

ORF Insert Sequence:

ATGATGTTCG ATTTTCTTTT ACAGATTTTT TTCATTTTCG TCGGGCCTTT CGTAACCGAC 
GCAAGTATTT CGACAACAAT GCGTTTTCAA AATCAAAAGT ACTATCATAT GCCTATTACT 
CTATCGAGAA GTCAGATACT GGAAATATCG GATTTGACAA ATGTCAATCA TATGGGCCAA 
ATTTTAGATA ACATATGTGT AGTGAGAATA GTCGGTACTC CAGAACATAC AAAAGTAAAA 
AATAACGATA TTAGTTTAAT GTTTATATTC TTTGACGGAG AAGAAGCTTT TAAAGAATGG 
GGTCCAAAAG ATTCCATATA CGGAGCAAGA CATTTAGCCA AAATTTGGCA TGATAATTAC 
ACCTCTTACA ACGAAGGAGA AAATATTTCA GAATTAGACA AAATTGATAT ATTGGTTCTT 
TTAGACCTGA TCGGCGCACC GGATCCAATG TTTTATAATT ATTTTAGCAA TACCGAAAAG 
TGGTATTCTT TATTAATGGC TATTGAAAGT AATTTAGCAG CTTTAAAAAA ATTTGAATCG 
TACTCGTACG AGCAACCTAC TCAGAGATAT TTTCAACCAC ATTCGATGGA AGCGCATATC 
GAAGATGATC ATATTCCGTT TTTGCAAAGA GACATTCCTA TTTTACACCT TATACCGTAT 
CCCTTTCCAA AAGTTTGGCA CAAACCAAGC GATCATTGTG ATAATATAGA TATAAAAACT 
ACGGAAAACA TTAATAAGAT TTTGAGGATT TTTGTAGCGT CGTATTTACA TATCAGTATG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.