LOC100876893 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100876893
Entrez Gene ID	100876893
Full Name	peflin
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012296659.1	XP_012152049.1	peflin isoform X1
XM_012296660.1	XP_012152050.1	peflin isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC100876893 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100876893 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe114594	XM_012296659.1 Latest version!	Megachile rotundata peflin (LOC100876893), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
OMe114595	XM_012296660.1 Latest version!	Megachile rotundata peflin (LOC100876893), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100876893 ( XM_012296659.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012152049.1 Megachile rotundata peflin (LOC100876893), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMe114594
Clone ID Related Accession (Same CDS sequence)	XM_012296659.1
Accession Version	XM_012296659.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	peflin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797877.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATATC CAGGCTCAAT GTATGGTGCA ACTGATCCAC AATCTCAAGT ATCTCCAGAA 
GTGCAACAGT GGTTTGCTGC TGTTGATAGA GACGGTAGTG GAAGAATAAC TGCTACAGAA 
TTAAAAGCTG CCTTAGCAAA TGGACAAGGA GGGACATTTT CAGATACTGC CTGCAAATTA 
ATGATTGGCA TGTTTGACAA AGAAAAGAGT GGTACAATAG ACTTAGTTGA ATTCCAAGCC 
CTTTATAATT ACATCAATGC ATGGTTAGGT GTTTTTCGTG GTTTCGATCA TGATAATTCA 
GGAACTATAC AGGAGAATGA ACTTAATGCA GCTTTAACAC AAATGGGATA TAGACTAAGT 
CCAGAATTTA TTTCATTTCT TATAAAAAAA AGTGATCCTA ATAGTCATTC TTGTATCACT 
ATTGATCAGT TCATTGTGTT ATGTGTTCAG ATTCAAAGAT TCACAGAAGC CTTTCGAGTA 
AGGGACTCTG ACCAAACAGG AACCATTACT ATCTCTTTTG AAGACTTTTT AGGTGTTGCA 
CTTAGCTGTA GTACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012152049.1
CDS	153..710
Translation	MAYPGSMYGATDPQSQVSPEVQQWFAAVDRDGSGRITATELKAALANGQGGTFSDTACKLMIGMFDKEKSGTIDLVEFQALYNYINAWLGVFRGFDHDNSGTIQENELNAALTQMGYRLSPEFISFLIKKSDPNSHSCITIDQFIVLCVQIQRFTEAFRVRDSDQTGTITISFEDFLGVALSCST

Target ORF information:

RefSeq Version	XM_012296659.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata peflin (LOC100876893), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012296659.1

ORF Insert Sequence:

ATGGCATATC CAGGCTCAAT GTATGGTGCA ACTGATCCAC AATCTCAAGT ATCTCCAGAA 
GTGCAACAGT GGTTTGCTGC TGTTGATAGA GACGGTAGTG GAAGAATAAC TGCTACAGAA 
TTAAAAGCTG CCTTAGCAAA TGGACAAGGA GGGACATTTT CAGATACTGC CTGCAAATTA 
ATGATTGGCA TGTTTGACAA AGAAAAGAGT GGTACAATAG ACTTAGTTGA ATTCCAAGCC 
CTTTATAATT ACATCAATGC ATGGTTAGGT GTTTTTCGTG GTTTCGATCA TGATAATTCA 
GGAACTATAC AGGAGAATGA ACTTAATGCA GCTTTAACAC AAATGGGATA TAGACTAAGT 
CCAGAATTTA TTTCATTTCT TATAAAAAAA AGTGATCCTA ATAGTCATTC TTGTATCACT 
ATTGATCAGT TCATTGTGTT ATGTGTTCAG ATTCAAAGAT TCACAGAAGC CTTTCGAGTA 
AGGGACTCTG ACCAAACAGG AACCATTACT ATCTCTTTTG AAGACTTTTT AGGTGTTGCA 
CTTAGCTGTA GTACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100876893 ( XM_012296660.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012152050.1 Megachile rotundata peflin (LOC100876893), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMe114595
Clone ID Related Accession (Same CDS sequence)	XM_012296660.1
Accession Version	XM_012296660.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	peflin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797877.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTCAA TGTATGGTGC AACTGATCCA CAATCTCAAG TATCTCCAGA AGTGCAACAG 
TGGTTTGCTG CTGTTGATAG AGACGGTAGT GGAAGAATAA CTGCTACAGA ATTAAAAGCT 
GCCTTAGCAA ATGGACAAGG AGGGACATTT TCAGATACTG CCTGCAAATT AATGATTGGC 
ATGTTTGACA AAGAAAAGAG TGGTACAATA GACTTAGTTG AATTCCAAGC CCTTTATAAT 
TACATCAATG CATGGTTAGG TGTTTTTCGT GGTTTCGATC ATGATAATTC AGGAACTATA 
CAGGAGAATG AACTTAATGC AGCTTTAACA CAAATGGGAT ATAGACTAAG TCCAGAATTT 
ATTTCATTTC TTATAAAAAA AAGTGATCCT AATAGTCATT CTTGTATCAC TATTGATCAG 
TTCATTGTGT TATGTGTTCA GATTCAAAGA TTCACAGAAG CCTTTCGAGT AAGGGACTCT 
GACCAAACAG GAACCATTAC TATCTCTTTT GAAGACTTTT TAGGTGTTGC ACTTAGCTGT 
AGTACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012152050.1
CDS	154..702
Translation	MGSMYGATDPQSQVSPEVQQWFAAVDRDGSGRITATELKAALANGQGGTFSDTACKLMIGMFDKEKSGTIDLVEFQALYNYINAWLGVFRGFDHDNSGTIQENELNAALTQMGYRLSPEFISFLIKKSDPNSHSCITIDQFIVLCVQIQRFTEAFRVRDSDQTGTITISFEDFLGVALSCST

Target ORF information:

RefSeq Version	XM_012296660.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata peflin (LOC100876893), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012296660.1

ORF Insert Sequence:

ATGGGCTCAA TGTATGGTGC AACTGATCCA CAATCTCAAG TATCTCCAGA AGTGCAACAG 
TGGTTTGCTG CTGTTGATAG AGACGGTAGT GGAAGAATAA CTGCTACAGA ATTAAAAGCT 
GCCTTAGCAA ATGGACAAGG AGGGACATTT TCAGATACTG CCTGCAAATT AATGATTGGC 
ATGTTTGACA AAGAAAAGAG TGGTACAATA GACTTAGTTG AATTCCAAGC CCTTTATAAT 
TACATCAATG CATGGTTAGG TGTTTTTCGT GGTTTCGATC ATGATAATTC AGGAACTATA 
CAGGAGAATG AACTTAATGC AGCTTTAACA CAAATGGGAT ATAGACTAAG TCCAGAATTT 
ATTTCATTTC TTATAAAAAA AAGTGATCCT AATAGTCATT CTTGTATCAC TATTGATCAG 
TTCATTGTGT TATGTGTTCA GATTCAAAGA TTCACAGAAG CCTTTCGAGT AAGGGACTCT 
GACCAAACAG GAACCATTAC TATCTCTTTT GAAGACTTTT TAGGTGTTGC ACTTAGCTGT 
AGTACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.