LOC100876742 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100876742
Entrez Gene ID	100876742
Full Name	AFG3-like protein 2
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012295494.1	XP_012150884.1	AFG3-like protein 2
XM_012295493.1	XP_012150883.1	AFG3-like protein 2
XM_012295495.1	XP_012150885.1	AFG3-like protein 2

Pathways from BioSystems
Gene Ontology

The following LOC100876742 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100876742 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMe113592	XM_012295493.1 Latest version!	Megachile rotundata AFG3-like protein 2 (LOC100876742), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OMe113592	XM_012295495.1 Latest version!	Megachile rotundata AFG3-like protein 2 (LOC100876742), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OMe113592	XM_012295494.1 Latest version!	Megachile rotundata AFG3-like protein 2 (LOC100876742), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100876742 ( XM_012295494.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012150884.1 Megachile rotundata AFG3-like protein 2 (LOC100876742), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMe113592
Clone ID Related Accession (Same CDS sequence)	XM_012295494.1 , XM_012295493.1 , XM_012295495.1
Accession Version	XM_012295494.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	AFG3-like protein 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797588.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACACC AGCTGTTATC TTCAACACGT AAATTAGAAA GATTTGTTTT AAATTCCATT 
TACAAAAATG CCAATATATT TCAGTTAAGG AGATACTTAT CATTGATTAG AAATGGCAAT 
GAAACAACTA CCATTGAACG GTTACAAAAA CAGTGGAAAT TGTGGAGTAA ACTTCCAAAA 
GGATTTGAAG AATTCTATAA ATCACCTGGA AGTAATACCA CTAAAACAAC TGAAAAAGTT 
GCACAGAAAG TTAAAAATGG ATCATCTAAA GAAGCAGCTG GTTCAAAATC ATCTGTACCT 
CCAGCTCAGA AACCATCTCT TGGTTCTTTA GGCTCTGAAA AACAGGATTT TAAACGAGAC 
TTTCAATGGG ACTTTAAATG GGCTGGAGGC AATAGTCCAT TTCCGAGCAA TAATAATGAA 
AAGATGCTTA TTATGGGTTT TATATTCTCA GTTACTGCTT ATTTATACAT GAATTATAGA 
ACAAATGCTA CAACAGAAAT CACATGGAGA GAATTCATAA CCAATTATTT AAACAAAGGA 
ATTGTGAATA AGGTAGAAGT TATTAATAAA CAGTGGGTTC GTGTAAAGTT AATACCTGGG 
CATGCAGAGG AAGGAAAAGA CGTTTTGTGG TTTAATATTG GAAGTGTGGA GACATTTGAA 
AGGAATTTGG AGAATGCACA ACTTGAGCTA AATGTAGATG TACAAAATTA TTTACCAGTT 
CATTATAAAG ATGAATTGGA GCTGCAGCAT GTTGTAAGAA TGCTTCCACA ATTAATAGTA 
TGGGGATTGT TGTTCGTATT ATTCCGAAAA TCAGTGGCAT CATTTGGCAA AGGAAGAAAA 
GGACTATTTG GTTCAGTGAT GGAATCAACT GCAAAATTAA TTAACTCTAA AGACATTGGT 
GTTCGATTTA AGGATGTTGC TGGTTGTGAA GAAGCAAAAC TTGAAATTAT GGAGTTTGTG 
AATTTTTTAA AAAATCCACA ACAATATATA GAACTTGGTG CTAAAATTCC CAAAGGCGCC 
ATGTTGACGG GACCACCTGG TACAGGCAAA ACGTTGTTAG CTAAGGCTAC AGCTGGTGAA 
GCAAATGTTC CTTTCATTAG TGTGTCAGGT TCTGAATTTC TGGAAATGTT TGTCGGTGTT 
GGTCCTTCGA GAGTTCGTGA CATGTTTGCA ATGGCACGAA AGCATGCTCC ATGCATATTA 
TTTATTGATG AAATCGATGC CGTTGGAAGG AAAAGAGGTG GTCGAAATTT CGGTGGTCAC 
TCGGAACAAG AAAATACACT TAATCAACTT TTAGTGGAAA TGGATGGATT TAATACAACT 
ACTAACGTAG TTGTGTTAGC GGCAACTAAT AGGATAGACA TTTTAGACAA AGCATTATTA 
CGTCCTGGAA GATTTGACAG ACAAATTTAC GTATCTGCGC CAGACATTAA GGGAAGAGCT 
TCTATTTTTA AAGTTCACTT AGCACCTTTA AAGGCAACGA TAGATAAAGA TCAGTTAGCT 
AGAAAAATGG CTTCCTTAAC ACCTGGTTTT ACCGGAGCCG ATATCGCCAA TGTATGTAAT 
GAAGCGGCTT TAATAGCGGC AAGAGACTTG AACGATAACA TACAATTAAA ACATTTCGAA 
CAAGCCATTG AAAGAGTTAT TGCTGGTATG GAAAAAAAGA CAAATGTCCT ACAGCCTGAA 
GAGAAAAAAA CAGTTGCATA TCATGAGGCC GGTCATGCGG TAGCAGGCTG GTTTTTAGAA 
TATGCAGATC CACTTTTAAA GGTTTCCATA ATTCCACGCG GTAAAGGTTT AGGCTACGCT 
CAGTATTTAC CACATGAACA ATATTTATAT ACAAAAGAGC AGTTGTTTGA TAGAATGTGT 
ATGGCACTCG GAGGACGGGT ATCCGAAGAA ATCTTTTTCG GTCGCATAAC GACAGGAGCA 
CAAGATGATT TACAGAAAGT AACATCAAGT GCATATGCTC AGGTTATTCA GTATGGTATG 
AATGAAAAAG TTGGTAATGT TAGTTTTCAA ATGCCACAAC AAGGTGATAT GACATTCGAT 
AAGCCATATT CTGAACATAC TGCACAACTC ATCGATACCG AAGTTCGTGC GTTAATTGAA 
AGAGCACATA CTCGAACACG TGAGCTACTC ACGGAACAGA AGGAAAATGT AACTAAGGTT 
GCTGAACGAT TATTAAAACA AGAAATTTTA AGTAGAGAGG ACATGATAGA ATTGCTTGGA 
CCTAGGCCGT TCCCTGAAAA ATCAACGTAT GAACAATTTG TCGAAGGAAC AGGTTCATTC 
GAGGAAGATA CCACTTTACC AAAAGGTTTA CAAGAATGGA ATAGATCGAG GGATGCGGAA 
AAGAGTGAAA AAGAAGTACC TGCAACGACG CAAGCAGTTT CAGATAGCAG TTCTCAAAAT 
CAACCGTCGC AGCGATTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012150884.1
CDS	322..2742
Translation	MAHQLLSSTRKLERFVLNSIYKNANIFQLRRYLSLIRNGNETTTIERLQKQWKLWSKLPKGFEEFYKSPGSNTTKTTEKVAQKVKNGSSKEAAGSKSSVPPAQKPSLGSLGSEKQDFKRDFQWDFKWAGGNSPFPSNNNEKMLIMGFIFSVTAYLYMNYRTNATTEITWREFITNYLNKGIVNKVEVINKQWVRVKLIPGHAEEGKDVLWFNIGSVETFERNLENAQLELNVDVQNYLPVHYKDELELQHVVRMLPQLIVWGLLFVLFRKSVASFGKGRKGLFGSVMESTAKLINSKDIGVRFKDVAGCEEAKLEIMEFVNFLKNPQQYIELGAKIPKGAMLTGPPGTGKTLLAKATAGEANVPFISVSGSEFLEMFVGVGPSRVRDMFAMARKHAPCILFIDEIDAVGRKRGGRNFGGHSEQENTLNQLLVEMDGFNTTTNVVVLAATNRIDILDKALLRPGRFDRQIYVSAPDIKGRASIFKVHLAPLKATIDKDQLARKMASLTPGFTGADIANVCNEAALIAARDLNDNIQLKHFEQAIERVIAGMEKKTNVLQPEEKKTVAYHEAGHAVAGWFLEYADPLLKVSIIPRGKGLGYAQYLPHEQYLYTKEQLFDRMCMALGGRVSEEIFFGRITTGAQDDLQKVTSSAYAQVIQYGMNEKVGNVSFQMPQQGDMTFDKPYSEHTAQLIDTEVRALIERAHTRTRELLTEQKENVTKVAERLLKQEILSREDMIELLGPRPFPEKSTYEQFVEGTGSFEEDTTLPKGLQEWNRSRDAEKSEKEVPATTQAVSDSSSQNQPSQRL

Target ORF information:

RefSeq Version	XM_012295494.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata AFG3-like protein 2 (LOC100876742), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012295494.1

ORF Insert Sequence:

ATGGCACACC AGCTGTTATC TTCAACACGT AAATTAGAAA GATTTGTTTT AAATTCCATT 
TACAAAAATG CCAATATATT TCAGTTAAGG AGATACTTAT CATTGATTAG AAATGGCAAT 
GAAACAACTA CCATTGAACG GTTACAAAAA CAGTGGAAAT TGTGGAGTAA ACTTCCAAAA 
GGATTTGAAG AATTCTATAA ATCACCTGGA AGTAATACCA CTAAAACAAC TGAAAAAGTT 
GCACAGAAAG TTAAAAATGG ATCATCTAAA GAAGCAGCTG GTTCAAAATC ATCTGTACCT 
CCAGCTCAGA AACCATCTCT TGGTTCTTTA GGCTCTGAAA AACAGGATTT TAAACGAGAC 
TTTCAATGGG ACTTTAAATG GGCTGGAGGC AATAGTCCAT TTCCGAGCAA TAATAATGAA 
AAGATGCTTA TTATGGGTTT TATATTCTCA GTTACTGCTT ATTTATACAT GAATTATAGA 
ACAAATGCTA CAACAGAAAT CACATGGAGA GAATTCATAA CCAATTATTT AAACAAAGGA 
ATTGTGAATA AGGTAGAAGT TATTAATAAA CAGTGGGTTC GTGTAAAGTT AATACCTGGG 
CATGCAGAGG AAGGAAAAGA CGTTTTGTGG TTTAATATTG GAAGTGTGGA GACATTTGAA 
AGGAATTTGG AGAATGCACA ACTTGAGCTA AATGTAGATG TACAAAATTA TTTACCAGTT 
CATTATAAAG ATGAATTGGA GCTGCAGCAT GTTGTAAGAA TGCTTCCACA ATTAATAGTA 
TGGGGATTGT TGTTCGTATT ATTCCGAAAA TCAGTGGCAT CATTTGGCAA AGGAAGAAAA 
GGACTATTTG GTTCAGTGAT GGAATCAACT GCAAAATTAA TTAACTCTAA AGACATTGGT 
GTTCGATTTA AGGATGTTGC TGGTTGTGAA GAAGCAAAAC TTGAAATTAT GGAGTTTGTG 
AATTTTTTAA AAAATCCACA ACAATATATA GAACTTGGTG CTAAAATTCC CAAAGGCGCC 
ATGTTGACGG GACCACCTGG TACAGGCAAA ACGTTGTTAG CTAAGGCTAC AGCTGGTGAA 
GCAAATGTTC CTTTCATTAG TGTGTCAGGT TCTGAATTTC TGGAAATGTT TGTCGGTGTT 
GGTCCTTCGA GAGTTCGTGA CATGTTTGCA ATGGCACGAA AGCATGCTCC ATGCATATTA 
TTTATTGATG AAATCGATGC CGTTGGAAGG AAAAGAGGTG GTCGAAATTT CGGTGGTCAC 
TCGGAACAAG AAAATACACT TAATCAACTT TTAGTGGAAA TGGATGGATT TAATACAACT 
ACTAACGTAG TTGTGTTAGC GGCAACTAAT AGGATAGACA TTTTAGACAA AGCATTATTA 
CGTCCTGGAA GATTTGACAG ACAAATTTAC GTATCTGCGC CAGACATTAA GGGAAGAGCT 
TCTATTTTTA AAGTTCACTT AGCACCTTTA AAGGCAACGA TAGATAAAGA TCAGTTAGCT 
AGAAAAATGG CTTCCTTAAC ACCTGGTTTT ACCGGAGCCG ATATCGCCAA TGTATGTAAT 
GAAGCGGCTT TAATAGCGGC AAGAGACTTG AACGATAACA TACAATTAAA ACATTTCGAA 
CAAGCCATTG AAAGAGTTAT TGCTGGTATG GAAAAAAAGA CAAATGTCCT ACAGCCTGAA 
GAGAAAAAAA CAGTTGCATA TCATGAGGCC GGTCATGCGG TAGCAGGCTG GTTTTTAGAA 
TATGCAGATC CACTTTTAAA GGTTTCCATA ATTCCACGCG GTAAAGGTTT AGGCTACGCT 
CAGTATTTAC CACATGAACA ATATTTATAT ACAAAAGAGC AGTTGTTTGA TAGAATGTGT 
ATGGCACTCG GAGGACGGGT ATCCGAAGAA ATCTTTTTCG GTCGCATAAC GACAGGAGCA 
CAAGATGATT TACAGAAAGT AACATCAAGT GCATATGCTC AGGTTATTCA GTATGGTATG 
AATGAAAAAG TTGGTAATGT TAGTTTTCAA ATGCCACAAC AAGGTGATAT GACATTCGAT 
AAGCCATATT CTGAACATAC TGCACAACTC ATCGATACCG AAGTTCGTGC GTTAATTGAA 
AGAGCACATA CTCGAACACG TGAGCTACTC ACGGAACAGA AGGAAAATGT AACTAAGGTT 
GCTGAACGAT TATTAAAACA AGAAATTTTA AGTAGAGAGG ACATGATAGA ATTGCTTGGA 
CCTAGGCCGT TCCCTGAAAA ATCAACGTAT GAACAATTTG TCGAAGGAAC AGGTTCATTC 
GAGGAAGATA CCACTTTACC AAAAGGTTTA CAAGAATGGA ATAGATCGAG GGATGCGGAA 
AAGAGTGAAA AAGAAGTACC TGCAACGACG CAAGCAGTTT CAGATAGCAG TTCTCAAAAT 
CAACCGTCGC AGCGATTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100876742 ( XM_012295493.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012150883.1 Megachile rotundata AFG3-like protein 2 (LOC100876742), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMe113592
Clone ID Related Accession (Same CDS sequence)	XM_012295494.1 , XM_012295493.1 , XM_012295495.1
Accession Version	XM_012295493.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	AFG3-like protein 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797588.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACACC AGCTGTTATC TTCAACACGT AAATTAGAAA GATTTGTTTT AAATTCCATT 
TACAAAAATG CCAATATATT TCAGTTAAGG AGATACTTAT CATTGATTAG AAATGGCAAT 
GAAACAACTA CCATTGAACG GTTACAAAAA CAGTGGAAAT TGTGGAGTAA ACTTCCAAAA 
GGATTTGAAG AATTCTATAA ATCACCTGGA AGTAATACCA CTAAAACAAC TGAAAAAGTT 
GCACAGAAAG TTAAAAATGG ATCATCTAAA GAAGCAGCTG GTTCAAAATC ATCTGTACCT 
CCAGCTCAGA AACCATCTCT TGGTTCTTTA GGCTCTGAAA AACAGGATTT TAAACGAGAC 
TTTCAATGGG ACTTTAAATG GGCTGGAGGC AATAGTCCAT TTCCGAGCAA TAATAATGAA 
AAGATGCTTA TTATGGGTTT TATATTCTCA GTTACTGCTT ATTTATACAT GAATTATAGA 
ACAAATGCTA CAACAGAAAT CACATGGAGA GAATTCATAA CCAATTATTT AAACAAAGGA 
ATTGTGAATA AGGTAGAAGT TATTAATAAA CAGTGGGTTC GTGTAAAGTT AATACCTGGG 
CATGCAGAGG AAGGAAAAGA CGTTTTGTGG TTTAATATTG GAAGTGTGGA GACATTTGAA 
AGGAATTTGG AGAATGCACA ACTTGAGCTA AATGTAGATG TACAAAATTA TTTACCAGTT 
CATTATAAAG ATGAATTGGA GCTGCAGCAT GTTGTAAGAA TGCTTCCACA ATTAATAGTA 
TGGGGATTGT TGTTCGTATT ATTCCGAAAA TCAGTGGCAT CATTTGGCAA AGGAAGAAAA 
GGACTATTTG GTTCAGTGAT GGAATCAACT GCAAAATTAA TTAACTCTAA AGACATTGGT 
GTTCGATTTA AGGATGTTGC TGGTTGTGAA GAAGCAAAAC TTGAAATTAT GGAGTTTGTG 
AATTTTTTAA AAAATCCACA ACAATATATA GAACTTGGTG CTAAAATTCC CAAAGGCGCC 
ATGTTGACGG GACCACCTGG TACAGGCAAA ACGTTGTTAG CTAAGGCTAC AGCTGGTGAA 
GCAAATGTTC CTTTCATTAG TGTGTCAGGT TCTGAATTTC TGGAAATGTT TGTCGGTGTT 
GGTCCTTCGA GAGTTCGTGA CATGTTTGCA ATGGCACGAA AGCATGCTCC ATGCATATTA 
TTTATTGATG AAATCGATGC CGTTGGAAGG AAAAGAGGTG GTCGAAATTT CGGTGGTCAC 
TCGGAACAAG AAAATACACT TAATCAACTT TTAGTGGAAA TGGATGGATT TAATACAACT 
ACTAACGTAG TTGTGTTAGC GGCAACTAAT AGGATAGACA TTTTAGACAA AGCATTATTA 
CGTCCTGGAA GATTTGACAG ACAAATTTAC GTATCTGCGC CAGACATTAA GGGAAGAGCT 
TCTATTTTTA AAGTTCACTT AGCACCTTTA AAGGCAACGA TAGATAAAGA TCAGTTAGCT 
AGAAAAATGG CTTCCTTAAC ACCTGGTTTT ACCGGAGCCG ATATCGCCAA TGTATGTAAT 
GAAGCGGCTT TAATAGCGGC AAGAGACTTG AACGATAACA TACAATTAAA ACATTTCGAA 
CAAGCCATTG AAAGAGTTAT TGCTGGTATG GAAAAAAAGA CAAATGTCCT ACAGCCTGAA 
GAGAAAAAAA CAGTTGCATA TCATGAGGCC GGTCATGCGG TAGCAGGCTG GTTTTTAGAA 
TATGCAGATC CACTTTTAAA GGTTTCCATA ATTCCACGCG GTAAAGGTTT AGGCTACGCT 
CAGTATTTAC CACATGAACA ATATTTATAT ACAAAAGAGC AGTTGTTTGA TAGAATGTGT 
ATGGCACTCG GAGGACGGGT ATCCGAAGAA ATCTTTTTCG GTCGCATAAC GACAGGAGCA 
CAAGATGATT TACAGAAAGT AACATCAAGT GCATATGCTC AGGTTATTCA GTATGGTATG 
AATGAAAAAG TTGGTAATGT TAGTTTTCAA ATGCCACAAC AAGGTGATAT GACATTCGAT 
AAGCCATATT CTGAACATAC TGCACAACTC ATCGATACCG AAGTTCGTGC GTTAATTGAA 
AGAGCACATA CTCGAACACG TGAGCTACTC ACGGAACAGA AGGAAAATGT AACTAAGGTT 
GCTGAACGAT TATTAAAACA AGAAATTTTA AGTAGAGAGG ACATGATAGA ATTGCTTGGA 
CCTAGGCCGT TCCCTGAAAA ATCAACGTAT GAACAATTTG TCGAAGGAAC AGGTTCATTC 
GAGGAAGATA CCACTTTACC AAAAGGTTTA CAAGAATGGA ATAGATCGAG GGATGCGGAA 
AAGAGTGAAA AAGAAGTACC TGCAACGACG CAAGCAGTTT CAGATAGCAG TTCTCAAAAT 
CAACCGTCGC AGCGATTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012150883.1
CDS	322..2742
Translation	MAHQLLSSTRKLERFVLNSIYKNANIFQLRRYLSLIRNGNETTTIERLQKQWKLWSKLPKGFEEFYKSPGSNTTKTTEKVAQKVKNGSSKEAAGSKSSVPPAQKPSLGSLGSEKQDFKRDFQWDFKWAGGNSPFPSNNNEKMLIMGFIFSVTAYLYMNYRTNATTEITWREFITNYLNKGIVNKVEVINKQWVRVKLIPGHAEEGKDVLWFNIGSVETFERNLENAQLELNVDVQNYLPVHYKDELELQHVVRMLPQLIVWGLLFVLFRKSVASFGKGRKGLFGSVMESTAKLINSKDIGVRFKDVAGCEEAKLEIMEFVNFLKNPQQYIELGAKIPKGAMLTGPPGTGKTLLAKATAGEANVPFISVSGSEFLEMFVGVGPSRVRDMFAMARKHAPCILFIDEIDAVGRKRGGRNFGGHSEQENTLNQLLVEMDGFNTTTNVVVLAATNRIDILDKALLRPGRFDRQIYVSAPDIKGRASIFKVHLAPLKATIDKDQLARKMASLTPGFTGADIANVCNEAALIAARDLNDNIQLKHFEQAIERVIAGMEKKTNVLQPEEKKTVAYHEAGHAVAGWFLEYADPLLKVSIIPRGKGLGYAQYLPHEQYLYTKEQLFDRMCMALGGRVSEEIFFGRITTGAQDDLQKVTSSAYAQVIQYGMNEKVGNVSFQMPQQGDMTFDKPYSEHTAQLIDTEVRALIERAHTRTRELLTEQKENVTKVAERLLKQEILSREDMIELLGPRPFPEKSTYEQFVEGTGSFEEDTTLPKGLQEWNRSRDAEKSEKEVPATTQAVSDSSSQNQPSQRL

Target ORF information:

RefSeq Version	XM_012295493.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata AFG3-like protein 2 (LOC100876742), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012295493.1

ORF Insert Sequence:

ATGGCACACC AGCTGTTATC TTCAACACGT AAATTAGAAA GATTTGTTTT AAATTCCATT 
TACAAAAATG CCAATATATT TCAGTTAAGG AGATACTTAT CATTGATTAG AAATGGCAAT 
GAAACAACTA CCATTGAACG GTTACAAAAA CAGTGGAAAT TGTGGAGTAA ACTTCCAAAA 
GGATTTGAAG AATTCTATAA ATCACCTGGA AGTAATACCA CTAAAACAAC TGAAAAAGTT 
GCACAGAAAG TTAAAAATGG ATCATCTAAA GAAGCAGCTG GTTCAAAATC ATCTGTACCT 
CCAGCTCAGA AACCATCTCT TGGTTCTTTA GGCTCTGAAA AACAGGATTT TAAACGAGAC 
TTTCAATGGG ACTTTAAATG GGCTGGAGGC AATAGTCCAT TTCCGAGCAA TAATAATGAA 
AAGATGCTTA TTATGGGTTT TATATTCTCA GTTACTGCTT ATTTATACAT GAATTATAGA 
ACAAATGCTA CAACAGAAAT CACATGGAGA GAATTCATAA CCAATTATTT AAACAAAGGA 
ATTGTGAATA AGGTAGAAGT TATTAATAAA CAGTGGGTTC GTGTAAAGTT AATACCTGGG 
CATGCAGAGG AAGGAAAAGA CGTTTTGTGG TTTAATATTG GAAGTGTGGA GACATTTGAA 
AGGAATTTGG AGAATGCACA ACTTGAGCTA AATGTAGATG TACAAAATTA TTTACCAGTT 
CATTATAAAG ATGAATTGGA GCTGCAGCAT GTTGTAAGAA TGCTTCCACA ATTAATAGTA 
TGGGGATTGT TGTTCGTATT ATTCCGAAAA TCAGTGGCAT CATTTGGCAA AGGAAGAAAA 
GGACTATTTG GTTCAGTGAT GGAATCAACT GCAAAATTAA TTAACTCTAA AGACATTGGT 
GTTCGATTTA AGGATGTTGC TGGTTGTGAA GAAGCAAAAC TTGAAATTAT GGAGTTTGTG 
AATTTTTTAA AAAATCCACA ACAATATATA GAACTTGGTG CTAAAATTCC CAAAGGCGCC 
ATGTTGACGG GACCACCTGG TACAGGCAAA ACGTTGTTAG CTAAGGCTAC AGCTGGTGAA 
GCAAATGTTC CTTTCATTAG TGTGTCAGGT TCTGAATTTC TGGAAATGTT TGTCGGTGTT 
GGTCCTTCGA GAGTTCGTGA CATGTTTGCA ATGGCACGAA AGCATGCTCC ATGCATATTA 
TTTATTGATG AAATCGATGC CGTTGGAAGG AAAAGAGGTG GTCGAAATTT CGGTGGTCAC 
TCGGAACAAG AAAATACACT TAATCAACTT TTAGTGGAAA TGGATGGATT TAATACAACT 
ACTAACGTAG TTGTGTTAGC GGCAACTAAT AGGATAGACA TTTTAGACAA AGCATTATTA 
CGTCCTGGAA GATTTGACAG ACAAATTTAC GTATCTGCGC CAGACATTAA GGGAAGAGCT 
TCTATTTTTA AAGTTCACTT AGCACCTTTA AAGGCAACGA TAGATAAAGA TCAGTTAGCT 
AGAAAAATGG CTTCCTTAAC ACCTGGTTTT ACCGGAGCCG ATATCGCCAA TGTATGTAAT 
GAAGCGGCTT TAATAGCGGC AAGAGACTTG AACGATAACA TACAATTAAA ACATTTCGAA 
CAAGCCATTG AAAGAGTTAT TGCTGGTATG GAAAAAAAGA CAAATGTCCT ACAGCCTGAA 
GAGAAAAAAA CAGTTGCATA TCATGAGGCC GGTCATGCGG TAGCAGGCTG GTTTTTAGAA 
TATGCAGATC CACTTTTAAA GGTTTCCATA ATTCCACGCG GTAAAGGTTT AGGCTACGCT 
CAGTATTTAC CACATGAACA ATATTTATAT ACAAAAGAGC AGTTGTTTGA TAGAATGTGT 
ATGGCACTCG GAGGACGGGT ATCCGAAGAA ATCTTTTTCG GTCGCATAAC GACAGGAGCA 
CAAGATGATT TACAGAAAGT AACATCAAGT GCATATGCTC AGGTTATTCA GTATGGTATG 
AATGAAAAAG TTGGTAATGT TAGTTTTCAA ATGCCACAAC AAGGTGATAT GACATTCGAT 
AAGCCATATT CTGAACATAC TGCACAACTC ATCGATACCG AAGTTCGTGC GTTAATTGAA 
AGAGCACATA CTCGAACACG TGAGCTACTC ACGGAACAGA AGGAAAATGT AACTAAGGTT 
GCTGAACGAT TATTAAAACA AGAAATTTTA AGTAGAGAGG ACATGATAGA ATTGCTTGGA 
CCTAGGCCGT TCCCTGAAAA ATCAACGTAT GAACAATTTG TCGAAGGAAC AGGTTCATTC 
GAGGAAGATA CCACTTTACC AAAAGGTTTA CAAGAATGGA ATAGATCGAG GGATGCGGAA 
AAGAGTGAAA AAGAAGTACC TGCAACGACG CAAGCAGTTT CAGATAGCAG TTCTCAAAAT 
CAACCGTCGC AGCGATTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100876742 ( XM_012295495.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012150885.1 Megachile rotundata AFG3-like protein 2 (LOC100876742), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMe113592
Clone ID Related Accession (Same CDS sequence)	XM_012295494.1 , XM_012295493.1 , XM_012295495.1
Accession Version	XM_012295495.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	AFG3-like protein 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797588.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACACC AGCTGTTATC TTCAACACGT AAATTAGAAA GATTTGTTTT AAATTCCATT 
TACAAAAATG CCAATATATT TCAGTTAAGG AGATACTTAT CATTGATTAG AAATGGCAAT 
GAAACAACTA CCATTGAACG GTTACAAAAA CAGTGGAAAT TGTGGAGTAA ACTTCCAAAA 
GGATTTGAAG AATTCTATAA ATCACCTGGA AGTAATACCA CTAAAACAAC TGAAAAAGTT 
GCACAGAAAG TTAAAAATGG ATCATCTAAA GAAGCAGCTG GTTCAAAATC ATCTGTACCT 
CCAGCTCAGA AACCATCTCT TGGTTCTTTA GGCTCTGAAA AACAGGATTT TAAACGAGAC 
TTTCAATGGG ACTTTAAATG GGCTGGAGGC AATAGTCCAT TTCCGAGCAA TAATAATGAA 
AAGATGCTTA TTATGGGTTT TATATTCTCA GTTACTGCTT ATTTATACAT GAATTATAGA 
ACAAATGCTA CAACAGAAAT CACATGGAGA GAATTCATAA CCAATTATTT AAACAAAGGA 
ATTGTGAATA AGGTAGAAGT TATTAATAAA CAGTGGGTTC GTGTAAAGTT AATACCTGGG 
CATGCAGAGG AAGGAAAAGA CGTTTTGTGG TTTAATATTG GAAGTGTGGA GACATTTGAA 
AGGAATTTGG AGAATGCACA ACTTGAGCTA AATGTAGATG TACAAAATTA TTTACCAGTT 
CATTATAAAG ATGAATTGGA GCTGCAGCAT GTTGTAAGAA TGCTTCCACA ATTAATAGTA 
TGGGGATTGT TGTTCGTATT ATTCCGAAAA TCAGTGGCAT CATTTGGCAA AGGAAGAAAA 
GGACTATTTG GTTCAGTGAT GGAATCAACT GCAAAATTAA TTAACTCTAA AGACATTGGT 
GTTCGATTTA AGGATGTTGC TGGTTGTGAA GAAGCAAAAC TTGAAATTAT GGAGTTTGTG 
AATTTTTTAA AAAATCCACA ACAATATATA GAACTTGGTG CTAAAATTCC CAAAGGCGCC 
ATGTTGACGG GACCACCTGG TACAGGCAAA ACGTTGTTAG CTAAGGCTAC AGCTGGTGAA 
GCAAATGTTC CTTTCATTAG TGTGTCAGGT TCTGAATTTC TGGAAATGTT TGTCGGTGTT 
GGTCCTTCGA GAGTTCGTGA CATGTTTGCA ATGGCACGAA AGCATGCTCC ATGCATATTA 
TTTATTGATG AAATCGATGC CGTTGGAAGG AAAAGAGGTG GTCGAAATTT CGGTGGTCAC 
TCGGAACAAG AAAATACACT TAATCAACTT TTAGTGGAAA TGGATGGATT TAATACAACT 
ACTAACGTAG TTGTGTTAGC GGCAACTAAT AGGATAGACA TTTTAGACAA AGCATTATTA 
CGTCCTGGAA GATTTGACAG ACAAATTTAC GTATCTGCGC CAGACATTAA GGGAAGAGCT 
TCTATTTTTA AAGTTCACTT AGCACCTTTA AAGGCAACGA TAGATAAAGA TCAGTTAGCT 
AGAAAAATGG CTTCCTTAAC ACCTGGTTTT ACCGGAGCCG ATATCGCCAA TGTATGTAAT 
GAAGCGGCTT TAATAGCGGC AAGAGACTTG AACGATAACA TACAATTAAA ACATTTCGAA 
CAAGCCATTG AAAGAGTTAT TGCTGGTATG GAAAAAAAGA CAAATGTCCT ACAGCCTGAA 
GAGAAAAAAA CAGTTGCATA TCATGAGGCC GGTCATGCGG TAGCAGGCTG GTTTTTAGAA 
TATGCAGATC CACTTTTAAA GGTTTCCATA ATTCCACGCG GTAAAGGTTT AGGCTACGCT 
CAGTATTTAC CACATGAACA ATATTTATAT ACAAAAGAGC AGTTGTTTGA TAGAATGTGT 
ATGGCACTCG GAGGACGGGT ATCCGAAGAA ATCTTTTTCG GTCGCATAAC GACAGGAGCA 
CAAGATGATT TACAGAAAGT AACATCAAGT GCATATGCTC AGGTTATTCA GTATGGTATG 
AATGAAAAAG TTGGTAATGT TAGTTTTCAA ATGCCACAAC AAGGTGATAT GACATTCGAT 
AAGCCATATT CTGAACATAC TGCACAACTC ATCGATACCG AAGTTCGTGC GTTAATTGAA 
AGAGCACATA CTCGAACACG TGAGCTACTC ACGGAACAGA AGGAAAATGT AACTAAGGTT 
GCTGAACGAT TATTAAAACA AGAAATTTTA AGTAGAGAGG ACATGATAGA ATTGCTTGGA 
CCTAGGCCGT TCCCTGAAAA ATCAACGTAT GAACAATTTG TCGAAGGAAC AGGTTCATTC 
GAGGAAGATA CCACTTTACC AAAAGGTTTA CAAGAATGGA ATAGATCGAG GGATGCGGAA 
AAGAGTGAAA AAGAAGTACC TGCAACGACG CAAGCAGTTT CAGATAGCAG TTCTCAAAAT 
CAACCGTCGC AGCGATTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012150885.1
CDS	322..2742
Translation	MAHQLLSSTRKLERFVLNSIYKNANIFQLRRYLSLIRNGNETTTIERLQKQWKLWSKLPKGFEEFYKSPGSNTTKTTEKVAQKVKNGSSKEAAGSKSSVPPAQKPSLGSLGSEKQDFKRDFQWDFKWAGGNSPFPSNNNEKMLIMGFIFSVTAYLYMNYRTNATTEITWREFITNYLNKGIVNKVEVINKQWVRVKLIPGHAEEGKDVLWFNIGSVETFERNLENAQLELNVDVQNYLPVHYKDELELQHVVRMLPQLIVWGLLFVLFRKSVASFGKGRKGLFGSVMESTAKLINSKDIGVRFKDVAGCEEAKLEIMEFVNFLKNPQQYIELGAKIPKGAMLTGPPGTGKTLLAKATAGEANVPFISVSGSEFLEMFVGVGPSRVRDMFAMARKHAPCILFIDEIDAVGRKRGGRNFGGHSEQENTLNQLLVEMDGFNTTTNVVVLAATNRIDILDKALLRPGRFDRQIYVSAPDIKGRASIFKVHLAPLKATIDKDQLARKMASLTPGFTGADIANVCNEAALIAARDLNDNIQLKHFEQAIERVIAGMEKKTNVLQPEEKKTVAYHEAGHAVAGWFLEYADPLLKVSIIPRGKGLGYAQYLPHEQYLYTKEQLFDRMCMALGGRVSEEIFFGRITTGAQDDLQKVTSSAYAQVIQYGMNEKVGNVSFQMPQQGDMTFDKPYSEHTAQLIDTEVRALIERAHTRTRELLTEQKENVTKVAERLLKQEILSREDMIELLGPRPFPEKSTYEQFVEGTGSFEEDTTLPKGLQEWNRSRDAEKSEKEVPATTQAVSDSSSQNQPSQRL

Target ORF information:

RefSeq Version	XM_012295495.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata AFG3-like protein 2 (LOC100876742), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012295495.1

ORF Insert Sequence:

ATGGCACACC AGCTGTTATC TTCAACACGT AAATTAGAAA GATTTGTTTT AAATTCCATT 
TACAAAAATG CCAATATATT TCAGTTAAGG AGATACTTAT CATTGATTAG AAATGGCAAT 
GAAACAACTA CCATTGAACG GTTACAAAAA CAGTGGAAAT TGTGGAGTAA ACTTCCAAAA 
GGATTTGAAG AATTCTATAA ATCACCTGGA AGTAATACCA CTAAAACAAC TGAAAAAGTT 
GCACAGAAAG TTAAAAATGG ATCATCTAAA GAAGCAGCTG GTTCAAAATC ATCTGTACCT 
CCAGCTCAGA AACCATCTCT TGGTTCTTTA GGCTCTGAAA AACAGGATTT TAAACGAGAC 
TTTCAATGGG ACTTTAAATG GGCTGGAGGC AATAGTCCAT TTCCGAGCAA TAATAATGAA 
AAGATGCTTA TTATGGGTTT TATATTCTCA GTTACTGCTT ATTTATACAT GAATTATAGA 
ACAAATGCTA CAACAGAAAT CACATGGAGA GAATTCATAA CCAATTATTT AAACAAAGGA 
ATTGTGAATA AGGTAGAAGT TATTAATAAA CAGTGGGTTC GTGTAAAGTT AATACCTGGG 
CATGCAGAGG AAGGAAAAGA CGTTTTGTGG TTTAATATTG GAAGTGTGGA GACATTTGAA 
AGGAATTTGG AGAATGCACA ACTTGAGCTA AATGTAGATG TACAAAATTA TTTACCAGTT 
CATTATAAAG ATGAATTGGA GCTGCAGCAT GTTGTAAGAA TGCTTCCACA ATTAATAGTA 
TGGGGATTGT TGTTCGTATT ATTCCGAAAA TCAGTGGCAT CATTTGGCAA AGGAAGAAAA 
GGACTATTTG GTTCAGTGAT GGAATCAACT GCAAAATTAA TTAACTCTAA AGACATTGGT 
GTTCGATTTA AGGATGTTGC TGGTTGTGAA GAAGCAAAAC TTGAAATTAT GGAGTTTGTG 
AATTTTTTAA AAAATCCACA ACAATATATA GAACTTGGTG CTAAAATTCC CAAAGGCGCC 
ATGTTGACGG GACCACCTGG TACAGGCAAA ACGTTGTTAG CTAAGGCTAC AGCTGGTGAA 
GCAAATGTTC CTTTCATTAG TGTGTCAGGT TCTGAATTTC TGGAAATGTT TGTCGGTGTT 
GGTCCTTCGA GAGTTCGTGA CATGTTTGCA ATGGCACGAA AGCATGCTCC ATGCATATTA 
TTTATTGATG AAATCGATGC CGTTGGAAGG AAAAGAGGTG GTCGAAATTT CGGTGGTCAC 
TCGGAACAAG AAAATACACT TAATCAACTT TTAGTGGAAA TGGATGGATT TAATACAACT 
ACTAACGTAG TTGTGTTAGC GGCAACTAAT AGGATAGACA TTTTAGACAA AGCATTATTA 
CGTCCTGGAA GATTTGACAG ACAAATTTAC GTATCTGCGC CAGACATTAA GGGAAGAGCT 
TCTATTTTTA AAGTTCACTT AGCACCTTTA AAGGCAACGA TAGATAAAGA TCAGTTAGCT 
AGAAAAATGG CTTCCTTAAC ACCTGGTTTT ACCGGAGCCG ATATCGCCAA TGTATGTAAT 
GAAGCGGCTT TAATAGCGGC AAGAGACTTG AACGATAACA TACAATTAAA ACATTTCGAA 
CAAGCCATTG AAAGAGTTAT TGCTGGTATG GAAAAAAAGA CAAATGTCCT ACAGCCTGAA 
GAGAAAAAAA CAGTTGCATA TCATGAGGCC GGTCATGCGG TAGCAGGCTG GTTTTTAGAA 
TATGCAGATC CACTTTTAAA GGTTTCCATA ATTCCACGCG GTAAAGGTTT AGGCTACGCT 
CAGTATTTAC CACATGAACA ATATTTATAT ACAAAAGAGC AGTTGTTTGA TAGAATGTGT 
ATGGCACTCG GAGGACGGGT ATCCGAAGAA ATCTTTTTCG GTCGCATAAC GACAGGAGCA 
CAAGATGATT TACAGAAAGT AACATCAAGT GCATATGCTC AGGTTATTCA GTATGGTATG 
AATGAAAAAG TTGGTAATGT TAGTTTTCAA ATGCCACAAC AAGGTGATAT GACATTCGAT 
AAGCCATATT CTGAACATAC TGCACAACTC ATCGATACCG AAGTTCGTGC GTTAATTGAA 
AGAGCACATA CTCGAACACG TGAGCTACTC ACGGAACAGA AGGAAAATGT AACTAAGGTT 
GCTGAACGAT TATTAAAACA AGAAATTTTA AGTAGAGAGG ACATGATAGA ATTGCTTGGA 
CCTAGGCCGT TCCCTGAAAA ATCAACGTAT GAACAATTTG TCGAAGGAAC AGGTTCATTC 
GAGGAAGATA CCACTTTACC AAAAGGTTTA CAAGAATGGA ATAGATCGAG GGATGCGGAA 
AAGAGTGAAA AAGAAGTACC TGCAACGACG CAAGCAGTTT CAGATAGCAG TTCTCAAAAT 
CAACCGTCGC AGCGATTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

[email protected]

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

LOC100876742 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム