LOC100876117 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100876117
Entrez Gene ID	100876117
Full Name	nucleolar GTP-binding protein 2
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012298432.1	XP_012153822.1	nucleolar GTP-binding protein 2

Pathways from BioSystems
Gene Ontology

The following LOC100876117 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100876117 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe98523	XM_012298432.1 Latest version!	Megachile rotundata nucleolar GTP-binding protein 2 (LOC100876117), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100876117 ( XM_012298432.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012153822.1 Megachile rotundata nucleolar GTP-binding protein 2 (LOC100876117), mRNA.

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CloneID	OMe98523
Clone ID Related Accession (Same CDS sequence)	XM_012298432.1
Accession Version	XM_012298432.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2118bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	nucleolar GTP-binding protein 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797090.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAGT CAAGAGCTAC AACTAAAGCT CCTCGTAAAG AGGGATTTAA TCGTTCTGGG 
CATAGCATGA ATCCTGAGCG CCCTACAGAA GGATTAAAAG GTGTAGCTAA AGTTCGGAGC 
AAAGCAACAA TCAAGAGATT ACAAATGTAT CGTAATCAAA AACCAAAACG TGATCGTACG 
GGTAAAATAA TTAGCCCAGC ACCATTCCAA GGTTGGCAAC CATCTGGTAC TATGTCTCGT 
ATAGAACCTT CGCATAGATG GTTTGGTAAT TCCAGAGTCA TTTCCCAGAA TGCTTTGCAA 
AAATTTCAAG ATGAATTAGG GAAAGCCATG AAAGATCCAT ATAATGTTAT TATGAAACCT 
ACACAAATGC CAGTAACTTT ACTTCAGCAA AAAGCTATGA ATGCAAGAGT ACACTTACTG 
GATACAGAAA GTTTTGAAAG TGTATTTGGT CCAAAAAAGT TACGTAAAAA ACCAAACTTA 
ACGATAGCTT CATATAATGA ATTACAAAAA TTAGCAGAAG AAAAGGAAGA AACATATAAC 
ATGGAAAAAG ATACAAAAGA CATTGATCTT GTTCGACCAG ATACAGGTAT AAAAGATATG 
CAGAGAGAAT GGATCATGTC AGCAGGACAA AGTAAAAGAA TTTGGAATGA ATTATATAAA 
GTTATAGATT CTTCTGATGT TATTTTACAA GTATTGGATG CCAGAGATCC TTTGGGCACA 
AGATCTCCAC CAGTAGAGAA ATATTTAAAG ACAGAAAAAC CTCATAAACA TTTAATGTTT 
ATTTTAAATA AGGTGGATCT TGTTCCAAAT TGGGTCACGC AAAGATGGGT TGCAATTTTA 
AGTGCAGAAT ATCCAACAGT AGCATTTCAT GCTTCAATGA CGCATCCATT TGGAAAAGGT 
TCCTTAATAA ATTTATTACG TCAATTTGCG AAATTACATA TGGATAAAAA ACAAATCAGT 
GTAGGTTTTA TAGGATATCC TAATACAGGG AAAAGTTCTG TTATAAATAC TTTGAGATCG 
AAAAAAGTAT GCAAAGTTGC GCCGATTGCC GGTGAAACAA AAGTATGGCA ATATATTACT 
TTAATGCGCC GTATTTACTT AATAGACTGT CCAGGAGTAG TTTATCCATC AGCGGAAACT 
GATGCAGAAA AAGTTTTGAA AGGTGTAGTT AGGGTAGAAC TTGTAAAAAA CCCTGAGGAT 
TATATTGAAG AAGTATTGTC ACGAGTAAAA CCAGATTATA TTCGCAAAAC TTATAAAATC 
ATGGAATGGA ATGATCACAT TGATTTTTTG GAGAAGTTGG CACGTAGAAC TGGAAAATTA 
TTGAAGAAAG GAGAACCAGA TATCACCATT GTGTCACGAA TGGTATTGAA TGATTGGCAA 
CGTGGTAAGC TACCATTTTA TGTCTCTCCG CCTGGTTTTG AAGTACCACT AACAAAAACA 
ACAGTGAATG AATCATCCAC TGAAAATAAT AATATTGAAC AAGAAAATGA AACTGAAACA 
AACGAAAATG AAACTGTACT AAATAAAGAA GTACCAAAAC CTGAATTATC TGTAATACAA 
GATTTTCGAA AAATAAGAGT TGGTTTACCA TATTCTGGTG ATGATATTCA AAATGAATTA 
CAGATTGAAG ACAATAAATC TATGAGTAAC TCAATTGAAA TTAATTCTAT TTCATCAGAC 
TTGGATGATA ATGATATTAG TGAAATTCAA GATGAAGCAA ATGTAAATTT TAAAGAAACA 
TTGTCAACAG AAAATAATGA ATGCAATAAA AATGATAAGG AAAATGACGA ATATTCTAAT 
GATAAAACTC ATCTTCCACA AGAAAAAGAT ACTTTAATGC AAAATGCATA TGATGTAATA 
GAAGAAGAAT ATGATTCTAG TGATAGTGAA ATGTTGGATA AAAATGCTGT ATCTAGTAGT 
GGTAAATTCA AAATTTCTTC TACATCTAAT GAAGTGGAAC ATATGACAGA AAAAGTTGGA 
AAAAAATTAA CAAGTAAACA AAGGCGAGCT CTAGAAAGAG CTAGTAAAAG GAAGAAAATT 
GGTAGCAATT TTTATGAAAT TACTAATGTG AAGAATAGGA ACAGGAACAG AAGAGTTCCT 
AAAATTAAAC GAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012153822.1
CDS	205..2322
Translation	MAKSRATTKAPRKEGFNRSGHSMNPERPTEGLKGVAKVRSKATIKRLQMYRNQKPKRDRTGKIISPAPFQGWQPSGTMSRIEPSHRWFGNSRVISQNALQKFQDELGKAMKDPYNVIMKPTQMPVTLLQQKAMNARVHLLDTESFESVFGPKKLRKKPNLTIASYNELQKLAEEKEETYNMEKDTKDIDLVRPDTGIKDMQREWIMSAGQSKRIWNELYKVIDSSDVILQVLDARDPLGTRSPPVEKYLKTEKPHKHLMFILNKVDLVPNWVTQRWVAILSAEYPTVAFHASMTHPFGKGSLINLLRQFAKLHMDKKQISVGFIGYPNTGKSSVINTLRSKKVCKVAPIAGETKVWQYITLMRRIYLIDCPGVVYPSAETDAEKVLKGVVRVELVKNPEDYIEEVLSRVKPDYIRKTYKIMEWNDHIDFLEKLARRTGKLLKKGEPDITIVSRMVLNDWQRGKLPFYVSPPGFEVPLTKTTVNESSTENNNIEQENETETNENETVLNKEVPKPELSVIQDFRKIRVGLPYSGDDIQNELQIEDNKSMSNSIEINSISSDLDDNDISEIQDEANVNFKETLSTENNECNKNDKENDEYSNDKTHLPQEKDTLMQNAYDVIEEEYDSSDSEMLDKNAVSSSGKFKISSTSNEVEHMTEKVGKKLTSKQRRALERASKRKKIGSNFYEITNVKNRNRNRRVPKIKRK

Target ORF information:

RefSeq Version	XM_012298432.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata nucleolar GTP-binding protein 2 (LOC100876117), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012298432.1

ORF Insert Sequence:

ATGGCAAAGT CAAGAGCTAC AACTAAAGCT CCTCGTAAAG AGGGATTTAA TCGTTCTGGG 
CATAGCATGA ATCCTGAGCG CCCTACAGAA GGATTAAAAG GTGTAGCTAA AGTTCGGAGC 
AAAGCAACAA TCAAGAGATT ACAAATGTAT CGTAATCAAA AACCAAAACG TGATCGTACG 
GGTAAAATAA TTAGCCCAGC ACCATTCCAA GGTTGGCAAC CATCTGGTAC TATGTCTCGT 
ATAGAACCTT CGCATAGATG GTTTGGTAAT TCCAGAGTCA TTTCCCAGAA TGCTTTGCAA 
AAATTTCAAG ATGAATTAGG GAAAGCCATG AAAGATCCAT ATAATGTTAT TATGAAACCT 
ACACAAATGC CAGTAACTTT ACTTCAGCAA AAAGCTATGA ATGCAAGAGT ACACTTACTG 
GATACAGAAA GTTTTGAAAG TGTATTTGGT CCAAAAAAGT TACGTAAAAA ACCAAACTTA 
ACGATAGCTT CATATAATGA ATTACAAAAA TTAGCAGAAG AAAAGGAAGA AACATATAAC 
ATGGAAAAAG ATACAAAAGA CATTGATCTT GTTCGACCAG ATACAGGTAT AAAAGATATG 
CAGAGAGAAT GGATCATGTC AGCAGGACAA AGTAAAAGAA TTTGGAATGA ATTATATAAA 
GTTATAGATT CTTCTGATGT TATTTTACAA GTATTGGATG CCAGAGATCC TTTGGGCACA 
AGATCTCCAC CAGTAGAGAA ATATTTAAAG ACAGAAAAAC CTCATAAACA TTTAATGTTT 
ATTTTAAATA AGGTGGATCT TGTTCCAAAT TGGGTCACGC AAAGATGGGT TGCAATTTTA 
AGTGCAGAAT ATCCAACAGT AGCATTTCAT GCTTCAATGA CGCATCCATT TGGAAAAGGT 
TCCTTAATAA ATTTATTACG TCAATTTGCG AAATTACATA TGGATAAAAA ACAAATCAGT 
GTAGGTTTTA TAGGATATCC TAATACAGGG AAAAGTTCTG TTATAAATAC TTTGAGATCG 
AAAAAAGTAT GCAAAGTTGC GCCGATTGCC GGTGAAACAA AAGTATGGCA ATATATTACT 
TTAATGCGCC GTATTTACTT AATAGACTGT CCAGGAGTAG TTTATCCATC AGCGGAAACT 
GATGCAGAAA AAGTTTTGAA AGGTGTAGTT AGGGTAGAAC TTGTAAAAAA CCCTGAGGAT 
TATATTGAAG AAGTATTGTC ACGAGTAAAA CCAGATTATA TTCGCAAAAC TTATAAAATC 
ATGGAATGGA ATGATCACAT TGATTTTTTG GAGAAGTTGG CACGTAGAAC TGGAAAATTA 
TTGAAGAAAG GAGAACCAGA TATCACCATT GTGTCACGAA TGGTATTGAA TGATTGGCAA 
CGTGGTAAGC TACCATTTTA TGTCTCTCCG CCTGGTTTTG AAGTACCACT AACAAAAACA 
ACAGTGAATG AATCATCCAC TGAAAATAAT AATATTGAAC AAGAAAATGA AACTGAAACA 
AACGAAAATG AAACTGTACT AAATAAAGAA GTACCAAAAC CTGAATTATC TGTAATACAA 
GATTTTCGAA AAATAAGAGT TGGTTTACCA TATTCTGGTG ATGATATTCA AAATGAATTA 
CAGATTGAAG ACAATAAATC TATGAGTAAC TCAATTGAAA TTAATTCTAT TTCATCAGAC 
TTGGATGATA ATGATATTAG TGAAATTCAA GATGAAGCAA ATGTAAATTT TAAAGAAACA 
TTGTCAACAG AAAATAATGA ATGCAATAAA AATGATAAGG AAAATGACGA ATATTCTAAT 
GATAAAACTC ATCTTCCACA AGAAAAAGAT ACTTTAATGC AAAATGCATA TGATGTAATA 
GAAGAAGAAT ATGATTCTAG TGATAGTGAA ATGTTGGATA AAAATGCTGT ATCTAGTAGT 
GGTAAATTCA AAATTTCTTC TACATCTAAT GAAGTGGAAC ATATGACAGA AAAAGTTGGA 
AAAAAATTAA CAAGTAAACA AAGGCGAGCT CTAGAAAGAG CTAGTAAAAG GAAGAAAATT 
GGTAGCAATT TTTATGAAAT TACTAATGTG AAGAATAGGA ACAGGAACAG AAGAGTTCCT 
AAAATTAAAC GAAAATAG