LOC100874615 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100874615
Entrez Gene ID	100874615
Full Name	probable phospholipid-transporting ATPase VA
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003701519.2	XP_003701567.1	probable phospholipid-transporting ATPase VD isoform X1
XM_012281194.1	XP_012136584.1	probable phospholipid-transporting ATPase VA isoform X4
XM_012281192.1	XP_012136582.1	probable phospholipid-transporting ATPase VA isoform X2
XM_012281195.1	XP_012136585.1	probable phospholipid-transporting ATPase VA isoform X4
XM_012281190.1	XP_012136580.1	probable phospholipid-transporting ATPase VD isoform X1
XM_012281193.1	XP_012136583.1	probable phospholipid-transporting ATPase VA isoform X3

Pathways from BioSystems
Gene Ontology

The following LOC100874615 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100874615 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMe101057	XM_012281190.1 Latest version!	Megachile rotundata probable phospholipid-transporting ATPase VA (LOC100874615), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OMe101057	XM_003701519.2 Latest version!	Megachile rotundata probable phospholipid-transporting ATPase VA (LOC100874615), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OMe101058	XM_012281192.1 Latest version!	Megachile rotundata probable phospholipid-transporting ATPase VA (LOC100874615), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OMe101059	XM_012281193.1 Latest version!	Megachile rotundata probable phospholipid-transporting ATPase VA (LOC100874615), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OMe101060	XM_012281195.1 Latest version!	Megachile rotundata probable phospholipid-transporting ATPase VA (LOC100874615), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OMe101060	XM_012281194.1 Latest version!	Megachile rotundata probable phospholipid-transporting ATPase VA (LOC100874615), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100874615 ( XM_003701519.2 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_003701567.1 Megachile rotundata probable phospholipid-transporting ATPase VA (LOC100874615), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMe101057
Clone ID Related Accession (Same CDS sequence)	XM_003701519.2 , XM_012281190.1
Accession Version	XM_003701519.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4443bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	probable phospholipid-transporting ATPase VD isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797118.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2015 this sequence version replaced XM_003701519.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGAAG CCGGCGCGCA AGAAGCTGCG AGCGAGATCG AGAGGCAGGC GTCGGTTTAC 
GTGTTCCCAG GACCGGGACC GAATCACCAG TCCTATCCCG GTTCGGCGTA CGCGTCGATG 
CAACCACCGA CCGCCTTGAT TCCCGATGTC TCGATCGCGA ACACGACCCC TTTGCCCGCA 
ACGTTCGGCA AGTTCGCGAA GACCGAGGTC GACGGGAACG AGGCGCCAGG GAACAGAATG 
AGACCGTGTC CGAGAACTCA CGCCAGGTCA GCCAGTCACG GAGGAGTATT GGCGGATTGT 
CTGACGAACA CTGGATACCA CGCACAGACG GGATCGTATC TTGGTCCCCT GTCAGCGATA 
GGAAGTGCCA GACCGTCAGC CTTGAAGAAA CCCGGTCACC AAAGGGCATT CAGTCAAGGT 
CAGGTAATCG AGGTACAGGG TCATTCAGTC ATGGGACACA GCCGAGTTGG CTCCAGAACG 
GACTTTATTC TTCCGCCTGG GCACAGAGAG GAATCCAGGC CACCCACTGC TGGAAAGGTG 
CCCTCCTTTC GAGGTCACTC GCGACAAGCG TCCAGATCAG AGTCGATATA CACGATAAGA 
CGTAGCGAAG AACCTCCTTG GTGGAGAAAA CTTTGGGCTC GATGTTTCGG TCCGTTACCC 
GATGAACCTC GCTTAAGGAC AATAGTACCA AATCATTTAA TATCGCCGAA AACACCTCCG 
AGTCAGCATC CTAATGGCAA AAGAGTGAAC AATAGAGTAC GCACAACAAA GTACACCACG 
CTGAGCTTCC TACCTCGCAA TCTTCTCGAA CAATTTCACC GTGTGGCCAA TATATATTTT 
GTGTTTATTG TACTGCTTAA TTGGGTGCCA GCTATAAACG CGTTCGGCAA AGAAGTAGCC 
ATGATACCTG TAATCTTCGT GCTGGGCGTG ACTGCACTAA AAGATTACTT CGAGGATCGC 
CGAAGACTGG CCAGCGATCG ACGCGTCAAT AATTCCACTT GTCGAGTTTA CGTCGGGGAG 
GACGACAGGT ATGCAAAGGT GGCGTGGAAA GATGTAAAGG TCGGTGACCT AGTTCATCTC 
TCCAACAACG AACTCGTGCC AGCCGATGTG TTGCTGTTAC GAAGCAGCGA TCCTCAGGGC 
ATTGCTTACA TCGACACGTG TAATCTCGAC GGTGAAACGA ATCTGAAACA ACGACAAGTC 
GTGAGAGGCT TCGTGGATTT TCAGGACACT TTTCAACCCG CCAAATTCCG ATCAGTAATC 
GAAGTCGATC AGCCGAGCAC CAGAATATAC AGGTTTCATG GAGCCGTCGT TCATCCGAAC 
GGAGGTAGAG TACCTGTTTC GACGGAGAAT CTTTTGCTCA GGGAGTGTTT GTTAAAGAAC 
ACCGATTTTG TCGAGGGAAT CGTGATATAC GCCGGTCACG AAACGAAAGC CTTGCTCAAT 
AACGGTGGAC CTCGGTACAA GTGTTCTCGC CTCGAGAAAC AAATGAACAG AGATGTCGTG 
TGGTGTGTGG TGATTTTGGT GGTGCTGTGC CTGATCGGTG CAATCGGTTG CCGATTTTGG 
CTCTCCAACT ATTCCGGTTT GTCGTTCGTA CCGTTTATTC CCGTTCTACA AGACCCAAAC 
TACGAGAGCA TGCTAACGTT TTGGACGTTC GTGATCATCC TTCAAGTGAT GATACCCTTG 
AGTCTATACG TCACTCTAGA AATGGCGAAA GTCGGCCAGG TTTATCACAT TGGTCACGAT 
GTCGCCCTGT ACGATGCGGA GACGGGACGC TCGGCTGAAT GTCGCGCGCT CAATATCACG 
GAGGAATTAG GTCAGGTGCA GTACATATTT TCTGATAAGA CGGGTACGCT GACGGAAAAC 
AAAATGCTGT TCAGACGATG TGTCGTGGGG GGTCAGGATT ATTCGCACAC GGGGGACGGT 
GAGAATTTGG TACCTTCCTC GCGGCTCAAG GAAGATCTCC TTATAGGTAC ATTTAGACAG 
CATCTACAAG AATTCCTAGT CGTATTAGCG ATATGTAATA CCGTTGTGGT CAATTCTCAA 
CCTCATTACG ACACTATGAA CTCTAGTGGA GTGATCGAGG ACCCTCAGCG GAACGGCGAT 
GAAACTGGAA GGTACACGAG AATGATAGAC TCAAGAAGTT TAACGCCTTC GCCAGTTATC 
CCTTTGTCCA CGCTCTCGCA TCCCACGGAT AGCCTCGACG AAAACGTGTC GTCGATTTCA 
TCGATGGTTG AAATCGAATC TGTTTTAAAT AGCACCTCGA AACTATCGAG TAAACTGCCT 
AGGCCAAAGT TTCTGAATGT GACGACGATA CCTAGTTTAG GATTGTTTGG AAGAAAATTG 
TCTCCGAACG GAGCGCAGAA AAGACAGGGT CAGCCGAGTC CCGTTACCGA TACGAACGGC 
AAGAACGGAG GCGAGCTGTT ACCTACCGCT GCGATTTACG AAGCGGAAAG CCCGGACGAG 
CTCGCCTTGG TGAACGCTGC TTGCGCTTAT GACGTGAAGC TTCTTAAACG AACAGCACGT 
AGCGCGATTG TCTGTTTGCC AGATAAATCT ATCCTTACTT TTGAGATTCT TCATGTTTTA 
CCATTCGATT CTAATAGAAA ATGTATGTCC ATCTTAGTAC GACATCCTCT GACGAACGAG 
CTTGTCTTGT ATAGCAAGGG TGCCGACACG TCTATACTGT CATCCCTAGT TTCTCAAGAA 
GAGAATTCCA TAACGACGAT TAAAGCTCGC CAGCATTTGC AATCTTATGC ACGTCAAGGT 
CTCCGAACCT TAGTGATGGC TAAAAAATCC CTGACTGCTC AGGAATACGA GACTTGGCGA 
CAAGCTCATA CAGAGGCGGA ATTGGCAATG GAAAATCGGG AATTGCGTAT CAAAAATTCG 
TACGCATCTC TTGAATCGCA TTTAACGCTT CTAGGTGCTA CTGGAATCGA AGACAAACTT 
CAAGCAGGTG TGCCTGAAAC AATGGCTACC CTAATGGCAG CAGGGATTGT AATTTGGGTG 
TTAACAGGAG ATAAACCAGA GACAGCAGTT AACATTGCAT ATTCAGCGCG TCTCTTTTCG 
CCAGCGATGC AATTATTGTG GTTGCAAGCA AGGTCCAAAA CCGTTGCAGA AACCTTGATC 
CGTGGATATT TAGAATCTGT TCGAAGAGAA AGTACGGCTC ACAGCAATGC ACGAAGCGGG 
TCGAATAACA GTAGAGATAT TACGATGCTT AATAATCGTG ATCCCGCGGA GAATGATGCT 
CATCGAATAG ACAGTCCATG GCCAAGACAA CGTGCACTCG TTGTAGATGG CAAGACACTG 
ACCGTTATTC TTGATCCACG ATCAGGATTG ATCCAACCAT TTCTTGAATT GACTCAAATG 
TGCTCCAGTG TGCTAGCATG TCGAGCAACG CCTCTTCAGA AGGCGTACAT AGTGAGGGTA 
GTAAAGGAAC AACTGGGAAT GAGAACTCTA GCAATTGGTG ATGGTGCGAA CGACGTTAGT 
ATGATTCAAA CAGCTGATGT GGGAGTGGGT ATCTCAGGAC ACGAAGGTAC GCAAGCTGTC 
ATGGCAGCGG ATTTTGCCGT TTCAAGATTC TCCATGTTGA GTAGACTTCT TTTATTGCAC 
GGACATTGGT GTTACGACCG TTTGTCCAGG ATGATCTTGT ACTTTTTCTA CAAGAATGCT 
ACCTTTGTCT TCCTTGTGTT TTGGTTTCAG TTGTACTGTG GCTTTACTGG ATCAGTTATG 
ATGGACCAAA TTTATCTGAT GTTGTACAAT TTGCTGTTCA CATCCATGCC ACCACTCATA 
TTGGGTGTGT ACGACCGAAT AGCTTCACCC GGTGTTTTGA TGTCTATGCC TCATCTCTAT 
AAAAGAGGAC GGCTTGGACT GGTTTACAAA CCATACTCGT TTTGGTTAAC CATCGTTGAT 
GCTTTGTATC AAAGTATCGT AATTTTTTTT GTAAATGTGG CTGTATACTA TGATTCCACC 
ATCGATATAT GGGAATTTGG AACTGCCGTA ATGACGTGTT GTATCGTTGT TATGTTAACA 
CACGTTGCCA TAGAAATTAG ATCTTGGACC ATAATTCATG TTTTTGCTGT AATGGGATCA 
TTAGGGATAT TCTTTGGATT CTGTTTAATT TATAACGTGG TTTGTGTTAA TTGCATGGGT 
CTGCCATGTT CATATTGGGT AATGGAAGTC GCCATCACCA GATACACATA TTGGCTTACA 
GTAGTGCTTA CCTGTGTTCT AGCATTATTA CCTAGGTTAT TTTATAAAAC GGTTAAATCC 
ACTATATTCC CAGATCTCAT GCAAAATGCT ACGTTTAATG TACAATTAAA AGGTGGTAGC 
CGCAGACAAC GAATCGCGGA AAGAAGAGAA AATAATTTTA TCGCTGGTTG GTCACGTTCT 
ACCCAACATG CTACCATTAG AACTGAAACC AAGAACGATG CATTGACAAC GATTGTTGCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003701567.1
CDS	813..5255
Translation	MSEAGAQEAASEIERQASVYVFPGPGPNHQSYPGSAYASMQPPTALIPDVSIANTTPLPATFGKFAKTEVDGNEAPGNRMRPCPRTHARSASHGGVLADCLTNTGYHAQTGSYLGPLSAIGSARPSALKKPGHQRAFSQGQVIEVQGHSVMGHSRVGSRTDFILPPGHREESRPPTAGKVPSFRGHSRQASRSESIYTIRRSEEPPWWRKLWARCFGPLPDEPRLRTIVPNHLISPKTPPSQHPNGKRVNNRVRTTKYTTLSFLPRNLLEQFHRVANIYFVFIVLLNWVPAINAFGKEVAMIPVIFVLGVTALKDYFEDRRRLASDRRVNNSTCRVYVGEDDRYAKVAWKDVKVGDLVHLSNNELVPADVLLLRSSDPQGIAYIDTCNLDGETNLKQRQVVRGFVDFQDTFQPAKFRSVIEVDQPSTRIYRFHGAVVHPNGGRVPVSTENLLLRECLLKNTDFVEGIVIYAGHETKALLNNGGPRYKCSRLEKQMNRDVVWCVVILVVLCLIGAIGCRFWLSNYSGLSFVPFIPVLQDPNYESMLTFWTFVIILQVMIPLSLYVTLEMAKVGQVYHIGHDVALYDAETGRSAECRALNITEELGQVQYIFSDKTGTLTENKMLFRRCVVGGQDYSHTGDGENLVPSSRLKEDLLIGTFRQHLQEFLVVLAICNTVVVNSQPHYDTMNSSGVIEDPQRNGDETGRYTRMIDSRSLTPSPVIPLSTLSHPTDSLDENVSSISSMVEIESVLNSTSKLSSKLPRPKFLNVTTIPSLGLFGRKLSPNGAQKRQGQPSPVTDTNGKNGGELLPTAAIYEAESPDELALVNAACAYDVKLLKRTARSAIVCLPDKSILTFEILHVLPFDSNRKCMSILVRHPLTNELVLYSKGADTSILSSLVSQEENSITTIKARQHLQSYARQGLRTLVMAKKSLTAQEYETWRQAHTEAELAMENRELRIKNSYASLESHLTLLGATGIEDKLQAGVPETMATLMAAGIVIWVLTGDKPETAVNIAYSARLFSPAMQLLWLQARSKTVAETLIRGYLESVRRESTAHSNARSGSNNSRDITMLNNRDPAENDAHRIDSPWPRQRALVVDGKTLTVILDPRSGLIQPFLELTQMCSSVLACRATPLQKAYIVRVVKEQLGMRTLAIGDGANDVSMIQTADVGVGISGHEGTQAVMAADFAVSRFSMLSRLLLLHGHWCYDRLSRMILYFFYKNATFVFLVFWFQLYCGFTGSVMMDQIYLMLYNLLFTSMPPLILGVYDRIASPGVLMSMPHLYKRGRLGLVYKPYSFWLTIVDALYQSIVIFFVNVAVYYDSTIDIWEFGTAVMTCCIVVMLTHVAIEIRSWTIIHVFAVMGSLGIFFGFCLIYNVVCVNCMGLPCSYWVMEVAITRYTYWLTVVLTCVLALLPRLFYKTVKSTIFPDLMQNATFNVQLKGGSRRQRIAERRENNFIAGWSRSTQHATIRTETKNDALTTIVA

Target ORF information:

RefSeq Version	XM_003701519.2
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata probable phospholipid-transporting ATPase VA (LOC100874615), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003701519.2

ORF Insert Sequence:

ATGTCCGAAG CCGGCGCGCA AGAAGCTGCG AGCGAGATCG AGAGGCAGGC GTCGGTTTAC 
GTGTTCCCAG GACCGGGACC GAATCACCAG TCCTATCCCG GTTCGGCGTA CGCGTCGATG 
CAACCACCGA CCGCCTTGAT TCCCGATGTC TCGATCGCGA ACACGACCCC TTTGCCCGCA 
ACGTTCGGCA AGTTCGCGAA GACCGAGGTC GACGGGAACG AGGCGCCAGG GAACAGAATG 
AGACCGTGTC CGAGAACTCA CGCCAGGTCA GCCAGTCACG GAGGAGTATT GGCGGATTGT 
CTGACGAACA CTGGATACCA CGCACAGACG GGATCGTATC TTGGTCCCCT GTCAGCGATA 
GGAAGTGCCA GACCGTCAGC CTTGAAGAAA CCCGGTCACC AAAGGGCATT CAGTCAAGGT 
CAGGTAATCG AGGTACAGGG TCATTCAGTC ATGGGACACA GCCGAGTTGG CTCCAGAACG 
GACTTTATTC TTCCGCCTGG GCACAGAGAG GAATCCAGGC CACCCACTGC TGGAAAGGTG 
CCCTCCTTTC GAGGTCACTC GCGACAAGCG TCCAGATCAG AGTCGATATA CACGATAAGA 
CGTAGCGAAG AACCTCCTTG GTGGAGAAAA CTTTGGGCTC GATGTTTCGG TCCGTTACCC 
GATGAACCTC GCTTAAGGAC AATAGTACCA AATCATTTAA TATCGCCGAA AACACCTCCG 
AGTCAGCATC CTAATGGCAA AAGAGTGAAC AATAGAGTAC GCACAACAAA GTACACCACG 
CTGAGCTTCC TACCTCGCAA TCTTCTCGAA CAATTTCACC GTGTGGCCAA TATATATTTT 
GTGTTTATTG TACTGCTTAA TTGGGTGCCA GCTATAAACG CGTTCGGCAA AGAAGTAGCC 
ATGATACCTG TAATCTTCGT GCTGGGCGTG ACTGCACTAA AAGATTACTT CGAGGATCGC 
CGAAGACTGG CCAGCGATCG ACGCGTCAAT AATTCCACTT GTCGAGTTTA CGTCGGGGAG 
GACGACAGGT ATGCAAAGGT GGCGTGGAAA GATGTAAAGG TCGGTGACCT AGTTCATCTC 
TCCAACAACG AACTCGTGCC AGCCGATGTG TTGCTGTTAC GAAGCAGCGA TCCTCAGGGC 
ATTGCTTACA TCGACACGTG TAATCTCGAC GGTGAAACGA ATCTGAAACA ACGACAAGTC 
GTGAGAGGCT TCGTGGATTT TCAGGACACT TTTCAACCCG CCAAATTCCG ATCAGTAATC 
GAAGTCGATC AGCCGAGCAC CAGAATATAC AGGTTTCATG GAGCCGTCGT TCATCCGAAC 
GGAGGTAGAG TACCTGTTTC GACGGAGAAT CTTTTGCTCA GGGAGTGTTT GTTAAAGAAC 
ACCGATTTTG TCGAGGGAAT CGTGATATAC GCCGGTCACG AAACGAAAGC CTTGCTCAAT 
AACGGTGGAC CTCGGTACAA GTGTTCTCGC CTCGAGAAAC AAATGAACAG AGATGTCGTG 
TGGTGTGTGG TGATTTTGGT GGTGCTGTGC CTGATCGGTG CAATCGGTTG CCGATTTTGG 
CTCTCCAACT ATTCCGGTTT GTCGTTCGTA CCGTTTATTC CCGTTCTACA AGACCCAAAC 
TACGAGAGCA TGCTAACGTT TTGGACGTTC GTGATCATCC TTCAAGTGAT GATACCCTTG 
AGTCTATACG TCACTCTAGA AATGGCGAAA GTCGGCCAGG TTTATCACAT TGGTCACGAT 
GTCGCCCTGT ACGATGCGGA GACGGGACGC TCGGCTGAAT GTCGCGCGCT CAATATCACG 
GAGGAATTAG GTCAGGTGCA GTACATATTT TCTGATAAGA CGGGTACGCT GACGGAAAAC 
AAAATGCTGT TCAGACGATG TGTCGTGGGG GGTCAGGATT ATTCGCACAC GGGGGACGGT 
GAGAATTTGG TACCTTCCTC GCGGCTCAAG GAAGATCTCC TTATAGGTAC ATTTAGACAG 
CATCTACAAG AATTCCTAGT CGTATTAGCG ATATGTAATA CCGTTGTGGT CAATTCTCAA 
CCTCATTACG ACACTATGAA CTCTAGTGGA GTGATCGAGG ACCCTCAGCG GAACGGCGAT 
GAAACTGGAA GGTACACGAG AATGATAGAC TCAAGAAGTT TAACGCCTTC GCCAGTTATC 
CCTTTGTCCA CGCTCTCGCA TCCCACGGAT AGCCTCGACG AAAACGTGTC GTCGATTTCA 
TCGATGGTTG AAATCGAATC TGTTTTAAAT AGCACCTCGA AACTATCGAG TAAACTGCCT 
AGGCCAAAGT TTCTGAATGT GACGACGATA CCTAGTTTAG GATTGTTTGG AAGAAAATTG 
TCTCCGAACG GAGCGCAGAA AAGACAGGGT CAGCCGAGTC CCGTTACCGA TACGAACGGC 
AAGAACGGAG GCGAGCTGTT ACCTACCGCT GCGATTTACG AAGCGGAAAG CCCGGACGAG 
CTCGCCTTGG TGAACGCTGC TTGCGCTTAT GACGTGAAGC TTCTTAAACG AACAGCACGT 
AGCGCGATTG TCTGTTTGCC AGATAAATCT ATCCTTACTT TTGAGATTCT TCATGTTTTA 
CCATTCGATT CTAATAGAAA ATGTATGTCC ATCTTAGTAC GACATCCTCT GACGAACGAG 
CTTGTCTTGT ATAGCAAGGG TGCCGACACG TCTATACTGT CATCCCTAGT TTCTCAAGAA 
GAGAATTCCA TAACGACGAT TAAAGCTCGC CAGCATTTGC AATCTTATGC ACGTCAAGGT 
CTCCGAACCT TAGTGATGGC TAAAAAATCC CTGACTGCTC AGGAATACGA GACTTGGCGA 
CAAGCTCATA CAGAGGCGGA ATTGGCAATG GAAAATCGGG AATTGCGTAT CAAAAATTCG 
TACGCATCTC TTGAATCGCA TTTAACGCTT CTAGGTGCTA CTGGAATCGA AGACAAACTT 
CAAGCAGGTG TGCCTGAAAC AATGGCTACC CTAATGGCAG CAGGGATTGT AATTTGGGTG 
TTAACAGGAG ATAAACCAGA GACAGCAGTT AACATTGCAT ATTCAGCGCG TCTCTTTTCG 
CCAGCGATGC AATTATTGTG GTTGCAAGCA AGGTCCAAAA CCGTTGCAGA AACCTTGATC 
CGTGGATATT TAGAATCTGT TCGAAGAGAA AGTACGGCTC ACAGCAATGC ACGAAGCGGG 
TCGAATAACA GTAGAGATAT TACGATGCTT AATAATCGTG ATCCCGCGGA GAATGATGCT 
CATCGAATAG ACAGTCCATG GCCAAGACAA CGTGCACTCG TTGTAGATGG CAAGACACTG 
ACCGTTATTC TTGATCCACG ATCAGGATTG ATCCAACCAT TTCTTGAATT GACTCAAATG 
TGCTCCAGTG TGCTAGCATG TCGAGCAACG CCTCTTCAGA AGGCGTACAT AGTGAGGGTA 
GTAAAGGAAC AACTGGGAAT GAGAACTCTA GCAATTGGTG ATGGTGCGAA CGACGTTAGT 
ATGATTCAAA CAGCTGATGT GGGAGTGGGT ATCTCAGGAC ACGAAGGTAC GCAAGCTGTC 
ATGGCAGCGG ATTTTGCCGT TTCAAGATTC TCCATGTTGA GTAGACTTCT TTTATTGCAC 
GGACATTGGT GTTACGACCG TTTGTCCAGG ATGATCTTGT ACTTTTTCTA CAAGAATGCT 
ACCTTTGTCT TCCTTGTGTT TTGGTTTCAG TTGTACTGTG GCTTTACTGG ATCAGTTATG 
ATGGACCAAA TTTATCTGAT GTTGTACAAT TTGCTGTTCA CATCCATGCC ACCACTCATA 
TTGGGTGTGT ACGACCGAAT AGCTTCACCC GGTGTTTTGA TGTCTATGCC TCATCTCTAT 
AAAAGAGGAC GGCTTGGACT GGTTTACAAA CCATACTCGT TTTGGTTAAC CATCGTTGAT 
GCTTTGTATC AAAGTATCGT AATTTTTTTT GTAAATGTGG CTGTATACTA TGATTCCACC 
ATCGATATAT GGGAATTTGG AACTGCCGTA ATGACGTGTT GTATCGTTGT TATGTTAACA 
CACGTTGCCA TAGAAATTAG ATCTTGGACC ATAATTCATG TTTTTGCTGT AATGGGATCA 
TTAGGGATAT TCTTTGGATT CTGTTTAATT TATAACGTGG TTTGTGTTAA TTGCATGGGT 
CTGCCATGTT CATATTGGGT AATGGAAGTC GCCATCACCA GATACACATA TTGGCTTACA 
GTAGTGCTTA CCTGTGTTCT AGCATTATTA CCTAGGTTAT TTTATAAAAC GGTTAAATCC 
ACTATATTCC CAGATCTCAT GCAAAATGCT ACGTTTAATG TACAATTAAA AGGTGGTAGC 
CGCAGACAAC GAATCGCGGA AAGAAGAGAA AATAATTTTA TCGCTGGTTG GTCACGTTCT 
ACCCAACATG CTACCATTAG AACTGAAACC AAGAACGATG CATTGACAAC GATTGTTGCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100874615 ( XM_012281190.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012136580.1 Megachile rotundata probable phospholipid-transporting ATPase VA (LOC100874615), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMe101057
Clone ID Related Accession (Same CDS sequence)	XM_003701519.2 , XM_012281190.1
Accession Version	XM_012281190.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4443bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	probable phospholipid-transporting ATPase VD isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797118.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGAAG CCGGCGCGCA AGAAGCTGCG AGCGAGATCG AGAGGCAGGC GTCGGTTTAC 
GTGTTCCCAG GACCGGGACC GAATCACCAG TCCTATCCCG GTTCGGCGTA CGCGTCGATG 
CAACCACCGA CCGCCTTGAT TCCCGATGTC TCGATCGCGA ACACGACCCC TTTGCCCGCA 
ACGTTCGGCA AGTTCGCGAA GACCGAGGTC GACGGGAACG AGGCGCCAGG GAACAGAATG 
AGACCGTGTC CGAGAACTCA CGCCAGGTCA GCCAGTCACG GAGGAGTATT GGCGGATTGT 
CTGACGAACA CTGGATACCA CGCACAGACG GGATCGTATC TTGGTCCCCT GTCAGCGATA 
GGAAGTGCCA GACCGTCAGC CTTGAAGAAA CCCGGTCACC AAAGGGCATT CAGTCAAGGT 
CAGGTAATCG AGGTACAGGG TCATTCAGTC ATGGGACACA GCCGAGTTGG CTCCAGAACG 
GACTTTATTC TTCCGCCTGG GCACAGAGAG GAATCCAGGC CACCCACTGC TGGAAAGGTG 
CCCTCCTTTC GAGGTCACTC GCGACAAGCG TCCAGATCAG AGTCGATATA CACGATAAGA 
CGTAGCGAAG AACCTCCTTG GTGGAGAAAA CTTTGGGCTC GATGTTTCGG TCCGTTACCC 
GATGAACCTC GCTTAAGGAC AATAGTACCA AATCATTTAA TATCGCCGAA AACACCTCCG 
AGTCAGCATC CTAATGGCAA AAGAGTGAAC AATAGAGTAC GCACAACAAA GTACACCACG 
CTGAGCTTCC TACCTCGCAA TCTTCTCGAA CAATTTCACC GTGTGGCCAA TATATATTTT 
GTGTTTATTG TACTGCTTAA TTGGGTGCCA GCTATAAACG CGTTCGGCAA AGAAGTAGCC 
ATGATACCTG TAATCTTCGT GCTGGGCGTG ACTGCACTAA AAGATTACTT CGAGGATCGC 
CGAAGACTGG CCAGCGATCG ACGCGTCAAT AATTCCACTT GTCGAGTTTA CGTCGGGGAG 
GACGACAGGT ATGCAAAGGT GGCGTGGAAA GATGTAAAGG TCGGTGACCT AGTTCATCTC 
TCCAACAACG AACTCGTGCC AGCCGATGTG TTGCTGTTAC GAAGCAGCGA TCCTCAGGGC 
ATTGCTTACA TCGACACGTG TAATCTCGAC GGTGAAACGA ATCTGAAACA ACGACAAGTC 
GTGAGAGGCT TCGTGGATTT TCAGGACACT TTTCAACCCG CCAAATTCCG ATCAGTAATC 
GAAGTCGATC AGCCGAGCAC CAGAATATAC AGGTTTCATG GAGCCGTCGT TCATCCGAAC 
GGAGGTAGAG TACCTGTTTC GACGGAGAAT CTTTTGCTCA GGGAGTGTTT GTTAAAGAAC 
ACCGATTTTG TCGAGGGAAT CGTGATATAC GCCGGTCACG AAACGAAAGC CTTGCTCAAT 
AACGGTGGAC CTCGGTACAA GTGTTCTCGC CTCGAGAAAC AAATGAACAG AGATGTCGTG 
TGGTGTGTGG TGATTTTGGT GGTGCTGTGC CTGATCGGTG CAATCGGTTG CCGATTTTGG 
CTCTCCAACT ATTCCGGTTT GTCGTTCGTA CCGTTTATTC CCGTTCTACA AGACCCAAAC 
TACGAGAGCA TGCTAACGTT TTGGACGTTC GTGATCATCC TTCAAGTGAT GATACCCTTG 
AGTCTATACG TCACTCTAGA AATGGCGAAA GTCGGCCAGG TTTATCACAT TGGTCACGAT 
GTCGCCCTGT ACGATGCGGA GACGGGACGC TCGGCTGAAT GTCGCGCGCT CAATATCACG 
GAGGAATTAG GTCAGGTGCA GTACATATTT TCTGATAAGA CGGGTACGCT GACGGAAAAC 
AAAATGCTGT TCAGACGATG TGTCGTGGGG GGTCAGGATT ATTCGCACAC GGGGGACGGT 
GAGAATTTGG TACCTTCCTC GCGGCTCAAG GAAGATCTCC TTATAGGTAC ATTTAGACAG 
CATCTACAAG AATTCCTAGT CGTATTAGCG ATATGTAATA CCGTTGTGGT CAATTCTCAA 
CCTCATTACG ACACTATGAA CTCTAGTGGA GTGATCGAGG ACCCTCAGCG GAACGGCGAT 
GAAACTGGAA GGTACACGAG AATGATAGAC TCAAGAAGTT TAACGCCTTC GCCAGTTATC 
CCTTTGTCCA CGCTCTCGCA TCCCACGGAT AGCCTCGACG AAAACGTGTC GTCGATTTCA 
TCGATGGTTG AAATCGAATC TGTTTTAAAT AGCACCTCGA AACTATCGAG TAAACTGCCT 
AGGCCAAAGT TTCTGAATGT GACGACGATA CCTAGTTTAG GATTGTTTGG AAGAAAATTG 
TCTCCGAACG GAGCGCAGAA AAGACAGGGT CAGCCGAGTC CCGTTACCGA TACGAACGGC 
AAGAACGGAG GCGAGCTGTT ACCTACCGCT GCGATTTACG AAGCGGAAAG CCCGGACGAG 
CTCGCCTTGG TGAACGCTGC TTGCGCTTAT GACGTGAAGC TTCTTAAACG AACAGCACGT 
AGCGCGATTG TCTGTTTGCC AGATAAATCT ATCCTTACTT TTGAGATTCT TCATGTTTTA 
CCATTCGATT CTAATAGAAA ATGTATGTCC ATCTTAGTAC GACATCCTCT GACGAACGAG 
CTTGTCTTGT ATAGCAAGGG TGCCGACACG TCTATACTGT CATCCCTAGT TTCTCAAGAA 
GAGAATTCCA TAACGACGAT TAAAGCTCGC CAGCATTTGC AATCTTATGC ACGTCAAGGT 
CTCCGAACCT TAGTGATGGC TAAAAAATCC CTGACTGCTC AGGAATACGA GACTTGGCGA 
CAAGCTCATA CAGAGGCGGA ATTGGCAATG GAAAATCGGG AATTGCGTAT CAAAAATTCG 
TACGCATCTC TTGAATCGCA TTTAACGCTT CTAGGTGCTA CTGGAATCGA AGACAAACTT 
CAAGCAGGTG TGCCTGAAAC AATGGCTACC CTAATGGCAG CAGGGATTGT AATTTGGGTG 
TTAACAGGAG ATAAACCAGA GACAGCAGTT AACATTGCAT ATTCAGCGCG TCTCTTTTCG 
CCAGCGATGC AATTATTGTG GTTGCAAGCA AGGTCCAAAA CCGTTGCAGA AACCTTGATC 
CGTGGATATT TAGAATCTGT TCGAAGAGAA AGTACGGCTC ACAGCAATGC ACGAAGCGGG 
TCGAATAACA GTAGAGATAT TACGATGCTT AATAATCGTG ATCCCGCGGA GAATGATGCT 
CATCGAATAG ACAGTCCATG GCCAAGACAA CGTGCACTCG TTGTAGATGG CAAGACACTG 
ACCGTTATTC TTGATCCACG ATCAGGATTG ATCCAACCAT TTCTTGAATT GACTCAAATG 
TGCTCCAGTG TGCTAGCATG TCGAGCAACG CCTCTTCAGA AGGCGTACAT AGTGAGGGTA 
GTAAAGGAAC AACTGGGAAT GAGAACTCTA GCAATTGGTG ATGGTGCGAA CGACGTTAGT 
ATGATTCAAA CAGCTGATGT GGGAGTGGGT ATCTCAGGAC ACGAAGGTAC GCAAGCTGTC 
ATGGCAGCGG ATTTTGCCGT TTCAAGATTC TCCATGTTGA GTAGACTTCT TTTATTGCAC 
GGACATTGGT GTTACGACCG TTTGTCCAGG ATGATCTTGT ACTTTTTCTA CAAGAATGCT 
ACCTTTGTCT TCCTTGTGTT TTGGTTTCAG TTGTACTGTG GCTTTACTGG ATCAGTTATG 
ATGGACCAAA TTTATCTGAT GTTGTACAAT TTGCTGTTCA CATCCATGCC ACCACTCATA 
TTGGGTGTGT ACGACCGAAT AGCTTCACCC GGTGTTTTGA TGTCTATGCC TCATCTCTAT 
AAAAGAGGAC GGCTTGGACT GGTTTACAAA CCATACTCGT TTTGGTTAAC CATCGTTGAT 
GCTTTGTATC AAAGTATCGT AATTTTTTTT GTAAATGTGG CTGTATACTA TGATTCCACC 
ATCGATATAT GGGAATTTGG AACTGCCGTA ATGACGTGTT GTATCGTTGT TATGTTAACA 
CACGTTGCCA TAGAAATTAG ATCTTGGACC ATAATTCATG TTTTTGCTGT AATGGGATCA 
TTAGGGATAT TCTTTGGATT CTGTTTAATT TATAACGTGG TTTGTGTTAA TTGCATGGGT 
CTGCCATGTT CATATTGGGT AATGGAAGTC GCCATCACCA GATACACATA TTGGCTTACA 
GTAGTGCTTA CCTGTGTTCT AGCATTATTA CCTAGGTTAT TTTATAAAAC GGTTAAATCC 
ACTATATTCC CAGATCTCAT GCAAAATGCT ACGTTTAATG TACAATTAAA AGGTGGTAGC 
CGCAGACAAC GAATCGCGGA AAGAAGAGAA AATAATTTTA TCGCTGGTTG GTCACGTTCT 
ACCCAACATG CTACCATTAG AACTGAAACC AAGAACGATG CATTGACAAC GATTGTTGCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012136580.1
CDS	544..4986
Translation	MSEAGAQEAASEIERQASVYVFPGPGPNHQSYPGSAYASMQPPTALIPDVSIANTTPLPATFGKFAKTEVDGNEAPGNRMRPCPRTHARSASHGGVLADCLTNTGYHAQTGSYLGPLSAIGSARPSALKKPGHQRAFSQGQVIEVQGHSVMGHSRVGSRTDFILPPGHREESRPPTAGKVPSFRGHSRQASRSESIYTIRRSEEPPWWRKLWARCFGPLPDEPRLRTIVPNHLISPKTPPSQHPNGKRVNNRVRTTKYTTLSFLPRNLLEQFHRVANIYFVFIVLLNWVPAINAFGKEVAMIPVIFVLGVTALKDYFEDRRRLASDRRVNNSTCRVYVGEDDRYAKVAWKDVKVGDLVHLSNNELVPADVLLLRSSDPQGIAYIDTCNLDGETNLKQRQVVRGFVDFQDTFQPAKFRSVIEVDQPSTRIYRFHGAVVHPNGGRVPVSTENLLLRECLLKNTDFVEGIVIYAGHETKALLNNGGPRYKCSRLEKQMNRDVVWCVVILVVLCLIGAIGCRFWLSNYSGLSFVPFIPVLQDPNYESMLTFWTFVIILQVMIPLSLYVTLEMAKVGQVYHIGHDVALYDAETGRSAECRALNITEELGQVQYIFSDKTGTLTENKMLFRRCVVGGQDYSHTGDGENLVPSSRLKEDLLIGTFRQHLQEFLVVLAICNTVVVNSQPHYDTMNSSGVIEDPQRNGDETGRYTRMIDSRSLTPSPVIPLSTLSHPTDSLDENVSSISSMVEIESVLNSTSKLSSKLPRPKFLNVTTIPSLGLFGRKLSPNGAQKRQGQPSPVTDTNGKNGGELLPTAAIYEAESPDELALVNAACAYDVKLLKRTARSAIVCLPDKSILTFEILHVLPFDSNRKCMSILVRHPLTNELVLYSKGADTSILSSLVSQEENSITTIKARQHLQSYARQGLRTLVMAKKSLTAQEYETWRQAHTEAELAMENRELRIKNSYASLESHLTLLGATGIEDKLQAGVPETMATLMAAGIVIWVLTGDKPETAVNIAYSARLFSPAMQLLWLQARSKTVAETLIRGYLESVRRESTAHSNARSGSNNSRDITMLNNRDPAENDAHRIDSPWPRQRALVVDGKTLTVILDPRSGLIQPFLELTQMCSSVLACRATPLQKAYIVRVVKEQLGMRTLAIGDGANDVSMIQTADVGVGISGHEGTQAVMAADFAVSRFSMLSRLLLLHGHWCYDRLSRMILYFFYKNATFVFLVFWFQLYCGFTGSVMMDQIYLMLYNLLFTSMPPLILGVYDRIASPGVLMSMPHLYKRGRLGLVYKPYSFWLTIVDALYQSIVIFFVNVAVYYDSTIDIWEFGTAVMTCCIVVMLTHVAIEIRSWTIIHVFAVMGSLGIFFGFCLIYNVVCVNCMGLPCSYWVMEVAITRYTYWLTVVLTCVLALLPRLFYKTVKSTIFPDLMQNATFNVQLKGGSRRQRIAERRENNFIAGWSRSTQHATIRTETKNDALTTIVA

Target ORF information:

RefSeq Version	XM_012281190.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata probable phospholipid-transporting ATPase VA (LOC100874615), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012281190.1

ORF Insert Sequence:

ATGTCCGAAG CCGGCGCGCA AGAAGCTGCG AGCGAGATCG AGAGGCAGGC GTCGGTTTAC 
GTGTTCCCAG GACCGGGACC GAATCACCAG TCCTATCCCG GTTCGGCGTA CGCGTCGATG 
CAACCACCGA CCGCCTTGAT TCCCGATGTC TCGATCGCGA ACACGACCCC TTTGCCCGCA 
ACGTTCGGCA AGTTCGCGAA GACCGAGGTC GACGGGAACG AGGCGCCAGG GAACAGAATG 
AGACCGTGTC CGAGAACTCA CGCCAGGTCA GCCAGTCACG GAGGAGTATT GGCGGATTGT 
CTGACGAACA CTGGATACCA CGCACAGACG GGATCGTATC TTGGTCCCCT GTCAGCGATA 
GGAAGTGCCA GACCGTCAGC CTTGAAGAAA CCCGGTCACC AAAGGGCATT CAGTCAAGGT 
CAGGTAATCG AGGTACAGGG TCATTCAGTC ATGGGACACA GCCGAGTTGG CTCCAGAACG 
GACTTTATTC TTCCGCCTGG GCACAGAGAG GAATCCAGGC CACCCACTGC TGGAAAGGTG 
CCCTCCTTTC GAGGTCACTC GCGACAAGCG TCCAGATCAG AGTCGATATA CACGATAAGA 
CGTAGCGAAG AACCTCCTTG GTGGAGAAAA CTTTGGGCTC GATGTTTCGG TCCGTTACCC 
GATGAACCTC GCTTAAGGAC AATAGTACCA AATCATTTAA TATCGCCGAA AACACCTCCG 
AGTCAGCATC CTAATGGCAA AAGAGTGAAC AATAGAGTAC GCACAACAAA GTACACCACG 
CTGAGCTTCC TACCTCGCAA TCTTCTCGAA CAATTTCACC GTGTGGCCAA TATATATTTT 
GTGTTTATTG TACTGCTTAA TTGGGTGCCA GCTATAAACG CGTTCGGCAA AGAAGTAGCC 
ATGATACCTG TAATCTTCGT GCTGGGCGTG ACTGCACTAA AAGATTACTT CGAGGATCGC 
CGAAGACTGG CCAGCGATCG ACGCGTCAAT AATTCCACTT GTCGAGTTTA CGTCGGGGAG 
GACGACAGGT ATGCAAAGGT GGCGTGGAAA GATGTAAAGG TCGGTGACCT AGTTCATCTC 
TCCAACAACG AACTCGTGCC AGCCGATGTG TTGCTGTTAC GAAGCAGCGA TCCTCAGGGC 
ATTGCTTACA TCGACACGTG TAATCTCGAC GGTGAAACGA ATCTGAAACA ACGACAAGTC 
GTGAGAGGCT TCGTGGATTT TCAGGACACT TTTCAACCCG CCAAATTCCG ATCAGTAATC 
GAAGTCGATC AGCCGAGCAC CAGAATATAC AGGTTTCATG GAGCCGTCGT TCATCCGAAC 
GGAGGTAGAG TACCTGTTTC GACGGAGAAT CTTTTGCTCA GGGAGTGTTT GTTAAAGAAC 
ACCGATTTTG TCGAGGGAAT CGTGATATAC GCCGGTCACG AAACGAAAGC CTTGCTCAAT 
AACGGTGGAC CTCGGTACAA GTGTTCTCGC CTCGAGAAAC AAATGAACAG AGATGTCGTG 
TGGTGTGTGG TGATTTTGGT GGTGCTGTGC CTGATCGGTG CAATCGGTTG CCGATTTTGG 
CTCTCCAACT ATTCCGGTTT GTCGTTCGTA CCGTTTATTC CCGTTCTACA AGACCCAAAC 
TACGAGAGCA TGCTAACGTT TTGGACGTTC GTGATCATCC TTCAAGTGAT GATACCCTTG 
AGTCTATACG TCACTCTAGA AATGGCGAAA GTCGGCCAGG TTTATCACAT TGGTCACGAT 
GTCGCCCTGT ACGATGCGGA GACGGGACGC TCGGCTGAAT GTCGCGCGCT CAATATCACG 
GAGGAATTAG GTCAGGTGCA GTACATATTT TCTGATAAGA CGGGTACGCT GACGGAAAAC 
AAAATGCTGT TCAGACGATG TGTCGTGGGG GGTCAGGATT ATTCGCACAC GGGGGACGGT 
GAGAATTTGG TACCTTCCTC GCGGCTCAAG GAAGATCTCC TTATAGGTAC ATTTAGACAG 
CATCTACAAG AATTCCTAGT CGTATTAGCG ATATGTAATA CCGTTGTGGT CAATTCTCAA 
CCTCATTACG ACACTATGAA CTCTAGTGGA GTGATCGAGG ACCCTCAGCG GAACGGCGAT 
GAAACTGGAA GGTACACGAG AATGATAGAC TCAAGAAGTT TAACGCCTTC GCCAGTTATC 
CCTTTGTCCA CGCTCTCGCA TCCCACGGAT AGCCTCGACG AAAACGTGTC GTCGATTTCA 
TCGATGGTTG AAATCGAATC TGTTTTAAAT AGCACCTCGA AACTATCGAG TAAACTGCCT 
AGGCCAAAGT TTCTGAATGT GACGACGATA CCTAGTTTAG GATTGTTTGG AAGAAAATTG 
TCTCCGAACG GAGCGCAGAA AAGACAGGGT CAGCCGAGTC CCGTTACCGA TACGAACGGC 
AAGAACGGAG GCGAGCTGTT ACCTACCGCT GCGATTTACG AAGCGGAAAG CCCGGACGAG 
CTCGCCTTGG TGAACGCTGC TTGCGCTTAT GACGTGAAGC TTCTTAAACG AACAGCACGT 
AGCGCGATTG TCTGTTTGCC AGATAAATCT ATCCTTACTT TTGAGATTCT TCATGTTTTA 
CCATTCGATT CTAATAGAAA ATGTATGTCC ATCTTAGTAC GACATCCTCT GACGAACGAG 
CTTGTCTTGT ATAGCAAGGG TGCCGACACG TCTATACTGT CATCCCTAGT TTCTCAAGAA 
GAGAATTCCA TAACGACGAT TAAAGCTCGC CAGCATTTGC AATCTTATGC ACGTCAAGGT 
CTCCGAACCT TAGTGATGGC TAAAAAATCC CTGACTGCTC AGGAATACGA GACTTGGCGA 
CAAGCTCATA CAGAGGCGGA ATTGGCAATG GAAAATCGGG AATTGCGTAT CAAAAATTCG 
TACGCATCTC TTGAATCGCA TTTAACGCTT CTAGGTGCTA CTGGAATCGA AGACAAACTT 
CAAGCAGGTG TGCCTGAAAC AATGGCTACC CTAATGGCAG CAGGGATTGT AATTTGGGTG 
TTAACAGGAG ATAAACCAGA GACAGCAGTT AACATTGCAT ATTCAGCGCG TCTCTTTTCG 
CCAGCGATGC AATTATTGTG GTTGCAAGCA AGGTCCAAAA CCGTTGCAGA AACCTTGATC 
CGTGGATATT TAGAATCTGT TCGAAGAGAA AGTACGGCTC ACAGCAATGC ACGAAGCGGG 
TCGAATAACA GTAGAGATAT TACGATGCTT AATAATCGTG ATCCCGCGGA GAATGATGCT 
CATCGAATAG ACAGTCCATG GCCAAGACAA CGTGCACTCG TTGTAGATGG CAAGACACTG 
ACCGTTATTC TTGATCCACG ATCAGGATTG ATCCAACCAT TTCTTGAATT GACTCAAATG 
TGCTCCAGTG TGCTAGCATG TCGAGCAACG CCTCTTCAGA AGGCGTACAT AGTGAGGGTA 
GTAAAGGAAC AACTGGGAAT GAGAACTCTA GCAATTGGTG ATGGTGCGAA CGACGTTAGT 
ATGATTCAAA CAGCTGATGT GGGAGTGGGT ATCTCAGGAC ACGAAGGTAC GCAAGCTGTC 
ATGGCAGCGG ATTTTGCCGT TTCAAGATTC TCCATGTTGA GTAGACTTCT TTTATTGCAC 
GGACATTGGT GTTACGACCG TTTGTCCAGG ATGATCTTGT ACTTTTTCTA CAAGAATGCT 
ACCTTTGTCT TCCTTGTGTT TTGGTTTCAG TTGTACTGTG GCTTTACTGG ATCAGTTATG 
ATGGACCAAA TTTATCTGAT GTTGTACAAT TTGCTGTTCA CATCCATGCC ACCACTCATA 
TTGGGTGTGT ACGACCGAAT AGCTTCACCC GGTGTTTTGA TGTCTATGCC TCATCTCTAT 
AAAAGAGGAC GGCTTGGACT GGTTTACAAA CCATACTCGT TTTGGTTAAC CATCGTTGAT 
GCTTTGTATC AAAGTATCGT AATTTTTTTT GTAAATGTGG CTGTATACTA TGATTCCACC 
ATCGATATAT GGGAATTTGG AACTGCCGTA ATGACGTGTT GTATCGTTGT TATGTTAACA 
CACGTTGCCA TAGAAATTAG ATCTTGGACC ATAATTCATG TTTTTGCTGT AATGGGATCA 
TTAGGGATAT TCTTTGGATT CTGTTTAATT TATAACGTGG TTTGTGTTAA TTGCATGGGT 
CTGCCATGTT CATATTGGGT AATGGAAGTC GCCATCACCA GATACACATA TTGGCTTACA 
GTAGTGCTTA CCTGTGTTCT AGCATTATTA CCTAGGTTAT TTTATAAAAC GGTTAAATCC 
ACTATATTCC CAGATCTCAT GCAAAATGCT ACGTTTAATG TACAATTAAA AGGTGGTAGC 
CGCAGACAAC GAATCGCGGA AAGAAGAGAA AATAATTTTA TCGCTGGTTG GTCACGTTCT 
ACCCAACATG CTACCATTAG AACTGAAACC AAGAACGATG CATTGACAAC GATTGTTGCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100874615 ( XM_012281192.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012136582.1 Megachile rotundata probable phospholipid-transporting ATPase VA (LOC100874615), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMe101058
Clone ID Related Accession (Same CDS sequence)	XM_012281192.1
Accession Version	XM_012281192.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	probable phospholipid-transporting ATPase VA isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797118.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGAAG CCGGCGCGCA AGAAGCTGCG AGCGAGATCG AGAGGCAGGC GTCGGTTTAC 
GTGTTCCCAG GACCGGGACC GAATCACCAG TCCTATCCCG GTTCGGCGTA CGCGTCGATG 
CAACCACCGA CCGCCTTGAT TCCCGATGTC TCGATCGCGA ACACGACCCC TTTGCCCGCA 
ACGTTCGGCA AGTTCGCGAA GACCGAGGTC GACGGGAACG AGGCGCCAGG GAACAGAATG 
AGACCGTGTC CGAGAACTCA CGCCAGGTCA GCCAGTCACG GAGGAGTATT GGCGGATTGT 
CTGACGAACA CTGGATACCA CGCACAGACG GGATCGTATC TTGGTCCCCT GTCAGCGATA 
GGAAGTGCCA GACCGTCAGC CTTGAAGAAA CCCGGTCACC AAAGGGCATT CAGTCAAGGT 
CAGGTAATCG AGGTACAGGG TCATTCAGTC ATGGGACACA GCCGAGTTGG CTCCAGAACG 
GACTTTATTC TTCCGCCTGG GCACAGAGAG GAATCCAGGC CACCCACTGC TGGAAAGGTG 
CCCTCCTTTC GAGGTCACTC GCGACAAGCG TCCAGATCAG AGTCGATATA CACGATAAGA 
CGTAGCGAAG AACCTCCTTG GTGGAGAAAA CTTTGGGCTC GATGTTTCGG TCCGTTACCC 
GATGAACCTC GCTTAAGGAC AATAGTACCA AATCATTTAA TATCGCCGAA AACACCTCCG 
AGTCAGCATC CTAATGGCAA AAGAGTGAAC AATAGAGTAC GCACAACAAA GTACACCACG 
CTGAGCTTCC TACCTCGCAA TCTTCTCGAA CAATTTCACC GTGTGGCCAA TATATATTTT 
GTGTTTATTG TACTGCTTAA TTGGGTGCCA GCTATAAACG CGTTCGGCAA AGAAGTAGCC 
ATGATACCTG TAATCTTCGT GCTGGGCGTG ACTGCACTAA AAGATTACTT CGAGGATCGC 
CGAAGACTGG CCAGCGATCG ACGCGTCAAT AATTCCACTT GTCGAGTTTA CGTCGGGGAG 
GACGACAGGT ATGCAAAGGT GGCGTGGAAA GATGTAAAGG TCGGTGACCT AGTTCATCTC 
TCCAACAACG AACTCGTGCC AGCCGATGTG TTGCTGTTAC GAAGCAGCGA TCCTCAGGGC 
ATTGCTTACA TCGACACGTG TAATCTCGAC GGTGAAACGA ATCTGAAACA ACGACAAGTC 
GTGAGAGGCT TCGTGGATTT TCAGGACACT TTTCAACCCG CCAAATTCCG ATCAGTAATC 
GAAGTCGATC AGCCGAGCAC CAGAATATAC AGGTTTCATG GAGCCGTCGT TCATCCGAAC 
GGAGGTAGAG TACCTGTTTC GACGGAGAAT CTTTTGCTCA GGGAGTGTTT GTTAAAGAAC 
ACCGATTTTG TCGAGGGAAT CGTGATATAC GCCGGTCACG AAACGAAAGC CTTGCTCAAT 
AACGGTGGAC CTCGGTACAA GTGTTCTCGC CTCGAGAAAC AAATGAACAG AGATGTCGTG 
TGGTGTGTGG TGATTTTGGT GGTGCTGTGC CTGATCGGTG CAATCGGTTG CCGATTTTGG 
CTCTCCAACT ATTCCGGTTT GTCGTTCGTA CCGTTTATTC CCGTTCTACA AGACCCAAAC 
TACGAGAGCA TGCTAACGTT TTGGACGTTC GTGATCATCC TTCAAGTGAT GATACCCTTG 
AGTCTATACG TCACTCTAGA AATGGCGAAA GTCGGCCAGG TTTATCACAT TGGTCACGAT 
GTCGCCCTGT ACGATGCGGA GACGGGACGC TCGGCTGAAT GTCGCGCGCT CAATATCACG 
GAGGAATTAG GTCAGGTGCA GTACATATTT TCTGATAAGA CGGGTACGCT GACGGAAAAC 
AAAATGCTGT TCAGACGATG TGTCGTGGGG GGTCAGGATT ATTCGCACAC GGGGGACGGT 
GAGAATTTGG TACCTTCCTC GCGGCTCAAG GAAGATCTCC TTATAGGTAC ATTTAGACAG 
CATCTACAAG AATTCCTAGT CGTATTAGCG ATATGTAATA CCGTTGTGGT CAATTCTCAA 
CCTCATTACG ACACTATGAA CTCTAGTGGA GTGATCGAGG ACCCTCAGCG GAACGGCGAT 
GAAACTGGAA GGTACACGAG AATGATAGAC TCAAGAAGTT TAACGCCTTC GCCAGTTATC 
CCTTTGTCCA CGCTCTCGCA TCCCACGGAT AGCCTCGACG AAAACGTGTC GTCGATTTCA 
TCGATGGTTG AAATCGAATC TGTTTTAAAT AGCACCTCGA AACTATCGAG TAAACTGCCT 
AGGCCAAAGT TTCTGAATGT GACGACGATA CCTAGTTTAG GATTGTTTGG AAGAAAATTG 
TCTCCGAACG GAGCGCAGAA AAGACAGGGT CAGCCGAGTC CCGTTACCGA TACGAACGGC 
AAGAACGGAG GCGAGCTGTT ACCTACCGCT GCGATTTACG AAGCGGAAAG CCCGGACGAG 
CTCGCCTTGG TGAACGCTGC TTGCGCTTAT GACGTGAAGC TTCTTAAACG AACAGCACGT 
AGCGCGATTG TCTGTTTGCC AGATAAATCT ATCCTTACTT TTGAGATTCT TCATGTTTTA 
CCATTCGATT CTAATAGAAA ATGTATGTCC ATCTTAGTAC GACATCCTCT GACGAACGAG 
CTTGTCTTGT ATAGCAAGGG TGCCGACACG TCTATACTGT CATCCCTAGT TTCTCAAGAA 
GAGAATTCCA TAACGACGAT TAAAGCTCGC CAGCATTTGC AATCTTATGC ACGTCAAGGT 
CTCCGAACCT TAGTGATGGC TAAAAAATCC CTGACTGCTC AGGAATACGA GACTTGGCGA 
CAAGCTCATA CAGAGGCGGA ATTGGCAATG GAAAATCGGG AATTGCGTAT CAAAAATTCG 
TACGCATCTC TTGAATCGCA TTTAACGCTT CTAGGTGCTA CTGGAATCGA AGACAAACTT 
CAAGCAGGTG TGCCTGAAAC AATGGCTACC CTAATGGCAG CAGGGATTGT AATTTGGGTG 
TTAACAGGAG ATAAACCAGA GACAGCAGTT AACATTGCAT ATTCAGCGCG TCTCTTTTCG 
CCAGCGATGC AATTATTGTG GTTGCAAGCA AGGTCCAAAA CCGTTGCAGA AACCTTGATC 
CGTGGATATT TAGAATCTGT TCGAAGAGAA AGTACGGCTC ACAGCAATGC ACGAAGCGGG 
TCGAATAACA GTAGAGATAT TACGATGCTT AATAATCGTG ATCCCGCGGA GAATGATGCT 
CATCGAATAG ACAGTCCATG GCCAAGACAA CGTGCACTCG TTGTAGATGG CAAGACACTG 
ACCGTTATTC TTGATCCACG ATCAGGATTG ATCCAACCAT TTCTTGAATT GACTCAAATG 
TGCTCCAGTG TGCTAGCATG TCGAGCAACG CCTCTTCAGA AGGCGTACAT AGTGAGGGTA 
GTAAAGGAAC AACTGGGAAT GAGAACTCTA GCAATTGGTG ATGGTGCGAA CGACGTTAGT 
ATGATTCAAA CAGCTGATGT GGGAGTGGGT ATCTCAGGAC ACGAAGGTAC GCAAGCTGTC 
ATGGCAGCGG ATTTTGCCGT TTCAAGATTC TCCATGTTGA GTAGACTTCT TTTATTGCAC 
GGACATTGGT GTTACGACCG TTTGTCCAGG ATGATCTTGT ACTTTTTCTA CAAGAATGCT 
ACCTTTGTCT TCCTTGTGTT TTGGTTTCAG TTGTACTGTG GCTTTACTGG ATCAGTTATG 
ATGGACCAAA TTTATCTGAT GTTGTACAAT TTGCTGTTCA CATCCATGCC ACCACTCATA 
TTGGGTGTGT ACGACCGAAT AGCTTCACCC GGTGTTTTGA TGTCTATGCC TCATCTCTAT 
AAAAGAGGAC GGCTTGGACT GGTTTACAAA CCATACTCGT TTTGGTTAAC CATCGTTGAT 
GCTTTGTATC AAAGTATCGT AATTTTTTTT GTAAATGTGG CTGTATACTA TGATTCCACC 
ATCGATATAT GGGAATTTGG AACTGCCGTA ATGACGTGTT GTATCGTTGT TATGTTAACA 
CACGTTGCCA TAGAAATTAG ATCTTGGACC ATAATTCATG TTTTTGCTGT AATGGGATCA 
TTAGGGATAT TCTTTGGATT CTGTTTAATT TATAACGTGG TTTGTGTTAA TTGCATGGGT 
CTGCCATGTT CATATTGGGT AATGGAAGTC GCCATCACCA GATACACATA TTGGCTTACA 
GTAGTGCTTA CCTGTGTTCT AGCATTATTA CCTAGATCTC ATGCAAAATG CTACGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012136582.1
CDS	544..4803
Translation	MSEAGAQEAASEIERQASVYVFPGPGPNHQSYPGSAYASMQPPTALIPDVSIANTTPLPATFGKFAKTEVDGNEAPGNRMRPCPRTHARSASHGGVLADCLTNTGYHAQTGSYLGPLSAIGSARPSALKKPGHQRAFSQGQVIEVQGHSVMGHSRVGSRTDFILPPGHREESRPPTAGKVPSFRGHSRQASRSESIYTIRRSEEPPWWRKLWARCFGPLPDEPRLRTIVPNHLISPKTPPSQHPNGKRVNNRVRTTKYTTLSFLPRNLLEQFHRVANIYFVFIVLLNWVPAINAFGKEVAMIPVIFVLGVTALKDYFEDRRRLASDRRVNNSTCRVYVGEDDRYAKVAWKDVKVGDLVHLSNNELVPADVLLLRSSDPQGIAYIDTCNLDGETNLKQRQVVRGFVDFQDTFQPAKFRSVIEVDQPSTRIYRFHGAVVHPNGGRVPVSTENLLLRECLLKNTDFVEGIVIYAGHETKALLNNGGPRYKCSRLEKQMNRDVVWCVVILVVLCLIGAIGCRFWLSNYSGLSFVPFIPVLQDPNYESMLTFWTFVIILQVMIPLSLYVTLEMAKVGQVYHIGHDVALYDAETGRSAECRALNITEELGQVQYIFSDKTGTLTENKMLFRRCVVGGQDYSHTGDGENLVPSSRLKEDLLIGTFRQHLQEFLVVLAICNTVVVNSQPHYDTMNSSGVIEDPQRNGDETGRYTRMIDSRSLTPSPVIPLSTLSHPTDSLDENVSSISSMVEIESVLNSTSKLSSKLPRPKFLNVTTIPSLGLFGRKLSPNGAQKRQGQPSPVTDTNGKNGGELLPTAAIYEAESPDELALVNAACAYDVKLLKRTARSAIVCLPDKSILTFEILHVLPFDSNRKCMSILVRHPLTNELVLYSKGADTSILSSLVSQEENSITTIKARQHLQSYARQGLRTLVMAKKSLTAQEYETWRQAHTEAELAMENRELRIKNSYASLESHLTLLGATGIEDKLQAGVPETMATLMAAGIVIWVLTGDKPETAVNIAYSARLFSPAMQLLWLQARSKTVAETLIRGYLESVRRESTAHSNARSGSNNSRDITMLNNRDPAENDAHRIDSPWPRQRALVVDGKTLTVILDPRSGLIQPFLELTQMCSSVLACRATPLQKAYIVRVVKEQLGMRTLAIGDGANDVSMIQTADVGVGISGHEGTQAVMAADFAVSRFSMLSRLLLLHGHWCYDRLSRMILYFFYKNATFVFLVFWFQLYCGFTGSVMMDQIYLMLYNLLFTSMPPLILGVYDRIASPGVLMSMPHLYKRGRLGLVYKPYSFWLTIVDALYQSIVIFFVNVAVYYDSTIDIWEFGTAVMTCCIVVMLTHVAIEIRSWTIIHVFAVMGSLGIFFGFCLIYNVVCVNCMGLPCSYWVMEVAITRYTYWLTVVLTCVLALLPRSHAKCYV

Target ORF information:

RefSeq Version	XM_012281192.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata probable phospholipid-transporting ATPase VA (LOC100874615), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012281192.1

ORF Insert Sequence:

ATGTCCGAAG CCGGCGCGCA AGAAGCTGCG AGCGAGATCG AGAGGCAGGC GTCGGTTTAC 
GTGTTCCCAG GACCGGGACC GAATCACCAG TCCTATCCCG GTTCGGCGTA CGCGTCGATG 
CAACCACCGA CCGCCTTGAT TCCCGATGTC TCGATCGCGA ACACGACCCC TTTGCCCGCA 
ACGTTCGGCA AGTTCGCGAA GACCGAGGTC GACGGGAACG AGGCGCCAGG GAACAGAATG 
AGACCGTGTC CGAGAACTCA CGCCAGGTCA GCCAGTCACG GAGGAGTATT GGCGGATTGT 
CTGACGAACA CTGGATACCA CGCACAGACG GGATCGTATC TTGGTCCCCT GTCAGCGATA 
GGAAGTGCCA GACCGTCAGC CTTGAAGAAA CCCGGTCACC AAAGGGCATT CAGTCAAGGT 
CAGGTAATCG AGGTACAGGG TCATTCAGTC ATGGGACACA GCCGAGTTGG CTCCAGAACG 
GACTTTATTC TTCCGCCTGG GCACAGAGAG GAATCCAGGC CACCCACTGC TGGAAAGGTG 
CCCTCCTTTC GAGGTCACTC GCGACAAGCG TCCAGATCAG AGTCGATATA CACGATAAGA 
CGTAGCGAAG AACCTCCTTG GTGGAGAAAA CTTTGGGCTC GATGTTTCGG TCCGTTACCC 
GATGAACCTC GCTTAAGGAC AATAGTACCA AATCATTTAA TATCGCCGAA AACACCTCCG 
AGTCAGCATC CTAATGGCAA AAGAGTGAAC AATAGAGTAC GCACAACAAA GTACACCACG 
CTGAGCTTCC TACCTCGCAA TCTTCTCGAA CAATTTCACC GTGTGGCCAA TATATATTTT 
GTGTTTATTG TACTGCTTAA TTGGGTGCCA GCTATAAACG CGTTCGGCAA AGAAGTAGCC 
ATGATACCTG TAATCTTCGT GCTGGGCGTG ACTGCACTAA AAGATTACTT CGAGGATCGC 
CGAAGACTGG CCAGCGATCG ACGCGTCAAT AATTCCACTT GTCGAGTTTA CGTCGGGGAG 
GACGACAGGT ATGCAAAGGT GGCGTGGAAA GATGTAAAGG TCGGTGACCT AGTTCATCTC 
TCCAACAACG AACTCGTGCC AGCCGATGTG TTGCTGTTAC GAAGCAGCGA TCCTCAGGGC 
ATTGCTTACA TCGACACGTG TAATCTCGAC GGTGAAACGA ATCTGAAACA ACGACAAGTC 
GTGAGAGGCT TCGTGGATTT TCAGGACACT TTTCAACCCG CCAAATTCCG ATCAGTAATC 
GAAGTCGATC AGCCGAGCAC CAGAATATAC AGGTTTCATG GAGCCGTCGT TCATCCGAAC 
GGAGGTAGAG TACCTGTTTC GACGGAGAAT CTTTTGCTCA GGGAGTGTTT GTTAAAGAAC 
ACCGATTTTG TCGAGGGAAT CGTGATATAC GCCGGTCACG AAACGAAAGC CTTGCTCAAT 
AACGGTGGAC CTCGGTACAA GTGTTCTCGC CTCGAGAAAC AAATGAACAG AGATGTCGTG 
TGGTGTGTGG TGATTTTGGT GGTGCTGTGC CTGATCGGTG CAATCGGTTG CCGATTTTGG 
CTCTCCAACT ATTCCGGTTT GTCGTTCGTA CCGTTTATTC CCGTTCTACA AGACCCAAAC 
TACGAGAGCA TGCTAACGTT TTGGACGTTC GTGATCATCC TTCAAGTGAT GATACCCTTG 
AGTCTATACG TCACTCTAGA AATGGCGAAA GTCGGCCAGG TTTATCACAT TGGTCACGAT 
GTCGCCCTGT ACGATGCGGA GACGGGACGC TCGGCTGAAT GTCGCGCGCT CAATATCACG 
GAGGAATTAG GTCAGGTGCA GTACATATTT TCTGATAAGA CGGGTACGCT GACGGAAAAC 
AAAATGCTGT TCAGACGATG TGTCGTGGGG GGTCAGGATT ATTCGCACAC GGGGGACGGT 
GAGAATTTGG TACCTTCCTC GCGGCTCAAG GAAGATCTCC TTATAGGTAC ATTTAGACAG 
CATCTACAAG AATTCCTAGT CGTATTAGCG ATATGTAATA CCGTTGTGGT CAATTCTCAA 
CCTCATTACG ACACTATGAA CTCTAGTGGA GTGATCGAGG ACCCTCAGCG GAACGGCGAT 
GAAACTGGAA GGTACACGAG AATGATAGAC TCAAGAAGTT TAACGCCTTC GCCAGTTATC 
CCTTTGTCCA CGCTCTCGCA TCCCACGGAT AGCCTCGACG AAAACGTGTC GTCGATTTCA 
TCGATGGTTG AAATCGAATC TGTTTTAAAT AGCACCTCGA AACTATCGAG TAAACTGCCT 
AGGCCAAAGT TTCTGAATGT GACGACGATA CCTAGTTTAG GATTGTTTGG AAGAAAATTG 
TCTCCGAACG GAGCGCAGAA AAGACAGGGT CAGCCGAGTC CCGTTACCGA TACGAACGGC 
AAGAACGGAG GCGAGCTGTT ACCTACCGCT GCGATTTACG AAGCGGAAAG CCCGGACGAG 
CTCGCCTTGG TGAACGCTGC TTGCGCTTAT GACGTGAAGC TTCTTAAACG AACAGCACGT 
AGCGCGATTG TCTGTTTGCC AGATAAATCT ATCCTTACTT TTGAGATTCT TCATGTTTTA 
CCATTCGATT CTAATAGAAA ATGTATGTCC ATCTTAGTAC GACATCCTCT GACGAACGAG 
CTTGTCTTGT ATAGCAAGGG TGCCGACACG TCTATACTGT CATCCCTAGT TTCTCAAGAA 
GAGAATTCCA TAACGACGAT TAAAGCTCGC CAGCATTTGC AATCTTATGC ACGTCAAGGT 
CTCCGAACCT TAGTGATGGC TAAAAAATCC CTGACTGCTC AGGAATACGA GACTTGGCGA 
CAAGCTCATA CAGAGGCGGA ATTGGCAATG GAAAATCGGG AATTGCGTAT CAAAAATTCG 
TACGCATCTC TTGAATCGCA TTTAACGCTT CTAGGTGCTA CTGGAATCGA AGACAAACTT 
CAAGCAGGTG TGCCTGAAAC AATGGCTACC CTAATGGCAG CAGGGATTGT AATTTGGGTG 
TTAACAGGAG ATAAACCAGA GACAGCAGTT AACATTGCAT ATTCAGCGCG TCTCTTTTCG 
CCAGCGATGC AATTATTGTG GTTGCAAGCA AGGTCCAAAA CCGTTGCAGA AACCTTGATC 
CGTGGATATT TAGAATCTGT TCGAAGAGAA AGTACGGCTC ACAGCAATGC ACGAAGCGGG 
TCGAATAACA GTAGAGATAT TACGATGCTT AATAATCGTG ATCCCGCGGA GAATGATGCT 
CATCGAATAG ACAGTCCATG GCCAAGACAA CGTGCACTCG TTGTAGATGG CAAGACACTG 
ACCGTTATTC TTGATCCACG ATCAGGATTG ATCCAACCAT TTCTTGAATT GACTCAAATG 
TGCTCCAGTG TGCTAGCATG TCGAGCAACG CCTCTTCAGA AGGCGTACAT AGTGAGGGTA 
GTAAAGGAAC AACTGGGAAT GAGAACTCTA GCAATTGGTG ATGGTGCGAA CGACGTTAGT 
ATGATTCAAA CAGCTGATGT GGGAGTGGGT ATCTCAGGAC ACGAAGGTAC GCAAGCTGTC 
ATGGCAGCGG ATTTTGCCGT TTCAAGATTC TCCATGTTGA GTAGACTTCT TTTATTGCAC 
GGACATTGGT GTTACGACCG TTTGTCCAGG ATGATCTTGT ACTTTTTCTA CAAGAATGCT 
ACCTTTGTCT TCCTTGTGTT TTGGTTTCAG TTGTACTGTG GCTTTACTGG ATCAGTTATG 
ATGGACCAAA TTTATCTGAT GTTGTACAAT TTGCTGTTCA CATCCATGCC ACCACTCATA 
TTGGGTGTGT ACGACCGAAT AGCTTCACCC GGTGTTTTGA TGTCTATGCC TCATCTCTAT 
AAAAGAGGAC GGCTTGGACT GGTTTACAAA CCATACTCGT TTTGGTTAAC CATCGTTGAT 
GCTTTGTATC AAAGTATCGT AATTTTTTTT GTAAATGTGG CTGTATACTA TGATTCCACC 
ATCGATATAT GGGAATTTGG AACTGCCGTA ATGACGTGTT GTATCGTTGT TATGTTAACA 
CACGTTGCCA TAGAAATTAG ATCTTGGACC ATAATTCATG TTTTTGCTGT AATGGGATCA 
TTAGGGATAT TCTTTGGATT CTGTTTAATT TATAACGTGG TTTGTGTTAA TTGCATGGGT 
CTGCCATGTT CATATTGGGT AATGGAAGTC GCCATCACCA GATACACATA TTGGCTTACA 
GTAGTGCTTA CCTGTGTTCT AGCATTATTA CCTAGATCTC ATGCAAAATG CTACGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100874615 ( XM_012281193.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012136583.1 Megachile rotundata probable phospholipid-transporting ATPase VA (LOC100874615), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMe101059
Clone ID Related Accession (Same CDS sequence)	XM_012281193.1
Accession Version	XM_012281193.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3924bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	probable phospholipid-transporting ATPase VA isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797118.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGAAG CCGGCGCGCA AGAAGCTGCG AGCGAGATCG AGAGGCAGGC GTCGGTTTAC 
GTGTTCCCAG GACCGGGACC GAATCACCAG TCCTATCCCG GTTCGGCGTA CGCGTCGATG 
CAACCACCGA CCGCCTTGAT TCCCGATGTC TCGATCGCGA ACACGACCCC TTTGCCCGCA 
ACGTTCGGCA AGTTCGCGAA GACCGAGGTC GACGGGAACG AGGCGCCAGG GAACAGAATG 
AGACCGTGTC CGAGAACTCA CGCCAGGTCA GCCAGTCACG GAGGAGTATT GGCGGATTGT 
CTGACGAACA CTGGATACCA CGCACAGACG GGATCGTATC TTGGTCCCCT GTCAGCGATA 
GGAAGTGCCA GACCGTCAGC CTTGAAGAAA CCCGGTCACC AAAGGGCATT CAGTCAAGGT 
CAGGTAATCG AGGTACAGGG TCATTCAGTC ATGGGACACA GCCGAGTTGG CTCCAGAACG 
GACTTTATTC TTCCGCCTGG GCACAGAGAG GAATCCAGGC CACCCACTGC TGGAAAGGTG 
CCCTCCTTTC GAGGTCACTC GCGACAAGCG TCCAGATCAG AGTCGATATA CACGATAAGA 
CGTAGCGAAG AACCTCCTTG GTGGAGAAAA CTTTGGGCTC GATGTTTCGG TCCGTTACCC 
GATGAACCTC GCTTAAGGAC AATAGTACCA AATCATTTAA TATCGCCGAA AACACCTCCG 
AGTCAGCATC CTAATGGCAA AAGAGTGAAC AATAGAGTAC GCACAACAAA GTACACCACG 
CTGAGCTTCC TACCTCGCAA TCTTCTCGAA CAATTTCACC GTGTGGCCAA TATATATTTT 
GTGTTTATTG TACTGCTTAA TTGGGTGCCA GCTATAAACG CGTTCGGCAA AGAAGTAGCC 
ATGATACCTG TAATCTTCGT GCTGGGCGTG ACTGCACTAA AAGATTACTT CGAGGATCGC 
CGAAGACTGG CCAGCGATCG ACGCGTCAAT AATTCCACTT GTCGAGTTTA CGTCGGGGAG 
GACGACAGGT ATGCAAAGGT GGCGTGGAAA GATGTAAAGG TCGGTGACCT AGTTCATCTC 
TCCAACAACG AACTCGTGCC AGCCGATGTG TTGCTGTTAC GAAGCAGCGA TCCTCAGGGC 
ATTGCTTACA TCGACACGTG TAATCTCGAC GGTGAAACGA ATCTGAAACA ACGACAAGTC 
GTGAGAGGCT TCGTGGATTT TCAGGACACT TTTCAACCCG CCAAATTCCG ATCAGTAATC 
GAAGTCGATC AGCCGAGCAC CAGAATATAC AGGTTTCATG GAGCCGTCGT TCATCCGAAC 
GGAGGTAGAG TACCTGTTTC GACGGAGAAT CTTTTGCTCA GGGAGTGTTT GTTAAAGAAC 
ACCGATTTTG TCGAGGGAAT CGTGATATAC GCCGGTCACG AAACGAAAGC CTTGCTCAAT 
AACGGTGGAC CTCGGTACAA GTGTTCTCGC CTCGAGAAAC AAATGAACAG AGATGTCGTG 
TGGTGTGTGG TGATTTTGGT GGTGCTGTGC CTGATCGGTG CAATCGGTTG CCGATTTTGG 
CTCTCCAACT ATTCCGGTTT GTCGTTCGTA CCGTTTATTC CCGTTCTACA AGACCCAAAC 
TACGAGAGCA TGCTAACGTT TTGGACGTTC GTGATCATCC TTCAAGTGAT GATACCCTTG 
AGTCTATACG TCACTCTAGA AATGGCGAAA GTCGGCCAGG TTTATCACAT TGGTCACGAT 
GTCGCCCTGT ACGATGCGGA GACGGGACGC TCGGCTGAAT GTCGCGCGCT CAATATCACG 
GAGGAATTAG GTCAGGTGCA GTACATATTT TCTGATAAGA CGGGTACGCT GACGGAAAAC 
AAAATGCTGT TCAGACGATG TGTCGTGGGG GGTCAGGATT ATTCGCACAC GGGGGACGGT 
GAGAATTTGG TACCTTCCTC GCGGCTCAAG GAAGATCTCC TTATAGGTAC ATTTAGACAG 
CATCTACAAG AATTCCTAGT CGTATTAGCG ATATGTAATA CCGTTGTGGT CAATTCTCAA 
CCTCATTACG ACACTATGAA CTCTAGTGGA GTGATCGAGG ACCCTCAGCG GAACGGCGAT 
GAAACTGGAA GGTACACGAG AATGATAGAC TCAAGAAGTT TAACGCCTTC GCCAGTTATC 
CCTTTGTCCA CGCTCTCGCA TCCCACGGAT AGCCTCGACG AAAACGTGTC GTCGATTTCA 
TCGATGGTTG AAATCGAATC TGTTTTAAAT AGCACCTCGA AACTATCGAG TAAACTGCCT 
AGGCCAAAGT TTCTGAATGT GACGACGATA CCTAGTTTAG GATTGTTTGG AAGAAAATTG 
TCTCCGAACG GAGCGCAGAA AAGACAGGGT CAGCCGAGTC CCGTTACCGA TACGAACGGC 
AAGAACGGAG GCGAGCTGTT ACCTACCGCT GCGATTTACG AAGCGGAAAG CCCGGACGAG 
CTCGCCTTGG TGAACGCTGC TTGCGCTTAT GACGTGAAGC TTCTTAAACG AACAGCACGT 
AGCGCGATTG TCTGTTTGCC AGATAAATCT ATCCTTACTT TTGAGATTCT TCATGTTTTA 
CCATTCGATT CTAATAGAAA ATGTATGTCC ATCTTAGTAC GACATCCTCT GACGAACGAG 
CTTGTCTTGT ATAGCAAGGG TGCCGACACG TCTATACTGT CATCCCTAGT TTCTCAAGAA 
GAGAATTCCA TAACGACGAT TAAAGCTCGC CAGCATTTGC AATCTTATGC ACGTCAAGGT 
CTCCGAACCT TAGTGATGGC TAAAAAATCC CTGACTGCTC AGGAATACGA GACTTGGCGA 
CAAGCTCATA CAGAGGCGGA ATTGGCAATG GAAAATCGGG AATTGCGTAT CAAAAATTCG 
TACGCATCTC TTGAATCGCA TTTAACGCTT CTAGGTGCTA CTGGAATCGA AGACAAACTT 
CAAGCAGGTG TGCCTGAAAC AATGGCTACC CTAATGGCAG CAGGGATTGT AATTTGGGTG 
TTAACAGGAG ATAAACCAGA GACAGCAGTT AACATTGCAT ATTCAGCGCG TCTCTTTTCG 
CCAGCGATGC AATTATTGTG GTTGCAAGCA AGGTCCAAAA CCGTTGCAGA AACCTTGATC 
CGTGGATATT TAGAATCTGT TCGAAGAGAA AGTACGGCTC ACAGCAATGC ACGAAGCGGG 
TCGAATAACA GTAGAGATAT TACGATGCTT AATAATCGTG ATCCCGCGGA GAATGATGCT 
CATCGAATAG ACAGTCCATG GCCAAGACAA CGTGCACTCG TTGTAGATGG CAAGACACTG 
ACCGTTATTC TTGATCCACG ATCAGGATTG ATCCAACCAT TTCTTGAATT GACTCAAATG 
TGCTCCAGTG TGCTAGCATG TCGAGCAACG CCTCTTCAGA AGGCGTACAT AGTGAGGGTA 
GTAAAGGAAC AACTGGGAAT GAGAACTCTA GCAATTGGTG ATGGTGCGAA CGACGTTAGT 
ATGATTCAAA CAGCTGATGT GGGAGTGGGT ATCTCAGGAC ACGAAGGTAC GCAAGCTGTC 
ATGGCAGCGG ATTTTGCCGT TTCAAGATTC TCCATGTTGA GTAGACTTCT TTTATTGCAC 
GGACATTGGT GTTACGACCG TTTGTCCAGG ATGATCTTGT ACTTTTTCTA CAAGAATGCT 
ACCTTTGTCT TCCTTGTGTT TTGGTTTCAG TTGTACTGTG GCTTTACTGG ATCAGTTATG 
ATGGACCAAA TTTATCTGAT GTTGTACAAT TTGCTGTTCA CATCCATGCC ACCACTCATA 
TTGGGTGTGT ACGACCGAAT AGCTTCACCC GGTGTTTTGA TGTCTATGCC TCATCTCTAT 
AAAAGAGGAC GGCTTGGACT GGTTTACAAA CCATACTCGT TTTGGTTAAC CATCGTTGAT 
GCTTTGTATC AAAGTATACT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012136583.1
CDS	544..4467
Translation	MSEAGAQEAASEIERQASVYVFPGPGPNHQSYPGSAYASMQPPTALIPDVSIANTTPLPATFGKFAKTEVDGNEAPGNRMRPCPRTHARSASHGGVLADCLTNTGYHAQTGSYLGPLSAIGSARPSALKKPGHQRAFSQGQVIEVQGHSVMGHSRVGSRTDFILPPGHREESRPPTAGKVPSFRGHSRQASRSESIYTIRRSEEPPWWRKLWARCFGPLPDEPRLRTIVPNHLISPKTPPSQHPNGKRVNNRVRTTKYTTLSFLPRNLLEQFHRVANIYFVFIVLLNWVPAINAFGKEVAMIPVIFVLGVTALKDYFEDRRRLASDRRVNNSTCRVYVGEDDRYAKVAWKDVKVGDLVHLSNNELVPADVLLLRSSDPQGIAYIDTCNLDGETNLKQRQVVRGFVDFQDTFQPAKFRSVIEVDQPSTRIYRFHGAVVHPNGGRVPVSTENLLLRECLLKNTDFVEGIVIYAGHETKALLNNGGPRYKCSRLEKQMNRDVVWCVVILVVLCLIGAIGCRFWLSNYSGLSFVPFIPVLQDPNYESMLTFWTFVIILQVMIPLSLYVTLEMAKVGQVYHIGHDVALYDAETGRSAECRALNITEELGQVQYIFSDKTGTLTENKMLFRRCVVGGQDYSHTGDGENLVPSSRLKEDLLIGTFRQHLQEFLVVLAICNTVVVNSQPHYDTMNSSGVIEDPQRNGDETGRYTRMIDSRSLTPSPVIPLSTLSHPTDSLDENVSSISSMVEIESVLNSTSKLSSKLPRPKFLNVTTIPSLGLFGRKLSPNGAQKRQGQPSPVTDTNGKNGGELLPTAAIYEAESPDELALVNAACAYDVKLLKRTARSAIVCLPDKSILTFEILHVLPFDSNRKCMSILVRHPLTNELVLYSKGADTSILSSLVSQEENSITTIKARQHLQSYARQGLRTLVMAKKSLTAQEYETWRQAHTEAELAMENRELRIKNSYASLESHLTLLGATGIEDKLQAGVPETMATLMAAGIVIWVLTGDKPETAVNIAYSARLFSPAMQLLWLQARSKTVAETLIRGYLESVRRESTAHSNARSGSNNSRDITMLNNRDPAENDAHRIDSPWPRQRALVVDGKTLTVILDPRSGLIQPFLELTQMCSSVLACRATPLQKAYIVRVVKEQLGMRTLAIGDGANDVSMIQTADVGVGISGHEGTQAVMAADFAVSRFSMLSRLLLLHGHWCYDRLSRMILYFFYKNATFVFLVFWFQLYCGFTGSVMMDQIYLMLYNLLFTSMPPLILGVYDRIASPGVLMSMPHLYKRGRLGLVYKPYSFWLTIVDALYQSIL

Target ORF information:

RefSeq Version	XM_012281193.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata probable phospholipid-transporting ATPase VA (LOC100874615), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012281193.1

ORF Insert Sequence:

ATGTCCGAAG CCGGCGCGCA AGAAGCTGCG AGCGAGATCG AGAGGCAGGC GTCGGTTTAC 
GTGTTCCCAG GACCGGGACC GAATCACCAG TCCTATCCCG GTTCGGCGTA CGCGTCGATG 
CAACCACCGA CCGCCTTGAT TCCCGATGTC TCGATCGCGA ACACGACCCC TTTGCCCGCA 
ACGTTCGGCA AGTTCGCGAA GACCGAGGTC GACGGGAACG AGGCGCCAGG GAACAGAATG 
AGACCGTGTC CGAGAACTCA CGCCAGGTCA GCCAGTCACG GAGGAGTATT GGCGGATTGT 
CTGACGAACA CTGGATACCA CGCACAGACG GGATCGTATC TTGGTCCCCT GTCAGCGATA 
GGAAGTGCCA GACCGTCAGC CTTGAAGAAA CCCGGTCACC AAAGGGCATT CAGTCAAGGT 
CAGGTAATCG AGGTACAGGG TCATTCAGTC ATGGGACACA GCCGAGTTGG CTCCAGAACG 
GACTTTATTC TTCCGCCTGG GCACAGAGAG GAATCCAGGC CACCCACTGC TGGAAAGGTG 
CCCTCCTTTC GAGGTCACTC GCGACAAGCG TCCAGATCAG AGTCGATATA CACGATAAGA 
CGTAGCGAAG AACCTCCTTG GTGGAGAAAA CTTTGGGCTC GATGTTTCGG TCCGTTACCC 
GATGAACCTC GCTTAAGGAC AATAGTACCA AATCATTTAA TATCGCCGAA AACACCTCCG 
AGTCAGCATC CTAATGGCAA AAGAGTGAAC AATAGAGTAC GCACAACAAA GTACACCACG 
CTGAGCTTCC TACCTCGCAA TCTTCTCGAA CAATTTCACC GTGTGGCCAA TATATATTTT 
GTGTTTATTG TACTGCTTAA TTGGGTGCCA GCTATAAACG CGTTCGGCAA AGAAGTAGCC 
ATGATACCTG TAATCTTCGT GCTGGGCGTG ACTGCACTAA AAGATTACTT CGAGGATCGC 
CGAAGACTGG CCAGCGATCG ACGCGTCAAT AATTCCACTT GTCGAGTTTA CGTCGGGGAG 
GACGACAGGT ATGCAAAGGT GGCGTGGAAA GATGTAAAGG TCGGTGACCT AGTTCATCTC 
TCCAACAACG AACTCGTGCC AGCCGATGTG TTGCTGTTAC GAAGCAGCGA TCCTCAGGGC 
ATTGCTTACA TCGACACGTG TAATCTCGAC GGTGAAACGA ATCTGAAACA ACGACAAGTC 
GTGAGAGGCT TCGTGGATTT TCAGGACACT TTTCAACCCG CCAAATTCCG ATCAGTAATC 
GAAGTCGATC AGCCGAGCAC CAGAATATAC AGGTTTCATG GAGCCGTCGT TCATCCGAAC 
GGAGGTAGAG TACCTGTTTC GACGGAGAAT CTTTTGCTCA GGGAGTGTTT GTTAAAGAAC 
ACCGATTTTG TCGAGGGAAT CGTGATATAC GCCGGTCACG AAACGAAAGC CTTGCTCAAT 
AACGGTGGAC CTCGGTACAA GTGTTCTCGC CTCGAGAAAC AAATGAACAG AGATGTCGTG 
TGGTGTGTGG TGATTTTGGT GGTGCTGTGC CTGATCGGTG CAATCGGTTG CCGATTTTGG 
CTCTCCAACT ATTCCGGTTT GTCGTTCGTA CCGTTTATTC CCGTTCTACA AGACCCAAAC 
TACGAGAGCA TGCTAACGTT TTGGACGTTC GTGATCATCC TTCAAGTGAT GATACCCTTG 
AGTCTATACG TCACTCTAGA AATGGCGAAA GTCGGCCAGG TTTATCACAT TGGTCACGAT 
GTCGCCCTGT ACGATGCGGA GACGGGACGC TCGGCTGAAT GTCGCGCGCT CAATATCACG 
GAGGAATTAG GTCAGGTGCA GTACATATTT TCTGATAAGA CGGGTACGCT GACGGAAAAC 
AAAATGCTGT TCAGACGATG TGTCGTGGGG GGTCAGGATT ATTCGCACAC GGGGGACGGT 
GAGAATTTGG TACCTTCCTC GCGGCTCAAG GAAGATCTCC TTATAGGTAC ATTTAGACAG 
CATCTACAAG AATTCCTAGT CGTATTAGCG ATATGTAATA CCGTTGTGGT CAATTCTCAA 
CCTCATTACG ACACTATGAA CTCTAGTGGA GTGATCGAGG ACCCTCAGCG GAACGGCGAT 
GAAACTGGAA GGTACACGAG AATGATAGAC TCAAGAAGTT TAACGCCTTC GCCAGTTATC 
CCTTTGTCCA CGCTCTCGCA TCCCACGGAT AGCCTCGACG AAAACGTGTC GTCGATTTCA 
TCGATGGTTG AAATCGAATC TGTTTTAAAT AGCACCTCGA AACTATCGAG TAAACTGCCT 
AGGCCAAAGT TTCTGAATGT GACGACGATA CCTAGTTTAG GATTGTTTGG AAGAAAATTG 
TCTCCGAACG GAGCGCAGAA AAGACAGGGT CAGCCGAGTC CCGTTACCGA TACGAACGGC 
AAGAACGGAG GCGAGCTGTT ACCTACCGCT GCGATTTACG AAGCGGAAAG CCCGGACGAG 
CTCGCCTTGG TGAACGCTGC TTGCGCTTAT GACGTGAAGC TTCTTAAACG AACAGCACGT 
AGCGCGATTG TCTGTTTGCC AGATAAATCT ATCCTTACTT TTGAGATTCT TCATGTTTTA 
CCATTCGATT CTAATAGAAA ATGTATGTCC ATCTTAGTAC GACATCCTCT GACGAACGAG 
CTTGTCTTGT ATAGCAAGGG TGCCGACACG TCTATACTGT CATCCCTAGT TTCTCAAGAA 
GAGAATTCCA TAACGACGAT TAAAGCTCGC CAGCATTTGC AATCTTATGC ACGTCAAGGT 
CTCCGAACCT TAGTGATGGC TAAAAAATCC CTGACTGCTC AGGAATACGA GACTTGGCGA 
CAAGCTCATA CAGAGGCGGA ATTGGCAATG GAAAATCGGG AATTGCGTAT CAAAAATTCG 
TACGCATCTC TTGAATCGCA TTTAACGCTT CTAGGTGCTA CTGGAATCGA AGACAAACTT 
CAAGCAGGTG TGCCTGAAAC AATGGCTACC CTAATGGCAG CAGGGATTGT AATTTGGGTG 
TTAACAGGAG ATAAACCAGA GACAGCAGTT AACATTGCAT ATTCAGCGCG TCTCTTTTCG 
CCAGCGATGC AATTATTGTG GTTGCAAGCA AGGTCCAAAA CCGTTGCAGA AACCTTGATC 
CGTGGATATT TAGAATCTGT TCGAAGAGAA AGTACGGCTC ACAGCAATGC ACGAAGCGGG 
TCGAATAACA GTAGAGATAT TACGATGCTT AATAATCGTG ATCCCGCGGA GAATGATGCT 
CATCGAATAG ACAGTCCATG GCCAAGACAA CGTGCACTCG TTGTAGATGG CAAGACACTG 
ACCGTTATTC TTGATCCACG ATCAGGATTG ATCCAACCAT TTCTTGAATT GACTCAAATG 
TGCTCCAGTG TGCTAGCATG TCGAGCAACG CCTCTTCAGA AGGCGTACAT AGTGAGGGTA 
GTAAAGGAAC AACTGGGAAT GAGAACTCTA GCAATTGGTG ATGGTGCGAA CGACGTTAGT 
ATGATTCAAA CAGCTGATGT GGGAGTGGGT ATCTCAGGAC ACGAAGGTAC GCAAGCTGTC 
ATGGCAGCGG ATTTTGCCGT TTCAAGATTC TCCATGTTGA GTAGACTTCT TTTATTGCAC 
GGACATTGGT GTTACGACCG TTTGTCCAGG ATGATCTTGT ACTTTTTCTA CAAGAATGCT 
ACCTTTGTCT TCCTTGTGTT TTGGTTTCAG TTGTACTGTG GCTTTACTGG ATCAGTTATG 
ATGGACCAAA TTTATCTGAT GTTGTACAAT TTGCTGTTCA CATCCATGCC ACCACTCATA 
TTGGGTGTGT ACGACCGAAT AGCTTCACCC GGTGTTTTGA TGTCTATGCC TCATCTCTAT 
AAAAGAGGAC GGCTTGGACT GGTTTACAAA CCATACTCGT TTTGGTTAAC CATCGTTGAT 
GCTTTGTATC AAAGTATACT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100874615 ( XM_012281194.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012136584.1 Megachile rotundata probable phospholipid-transporting ATPase VA (LOC100874615), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMe101060
Clone ID Related Accession (Same CDS sequence)	XM_012281194.1 , XM_012281195.1
Accession Version	XM_012281194.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	probable phospholipid-transporting ATPase VA isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797118.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGAAG CCGGCGCGCA AGAAGCTGCG AGCGAGATCG AGAGGCAGGC GTCGGTTTAC 
GTGTTCCCAG GACCGGGACC GAATCACCAG TCCTATCCCG GTTCGGCGTA CGCGTCGATG 
CAACCACCGA CCGCCTTGAT TCCCGATGTC TCGATCGCGA ACACGACCCC TTTGCCCGCA 
ACGTTCGGCA AGTTCGCGAA GACCGAGGTC GACGGGAACG AGGCGCCAGG GAACAGAATG 
AGACCGTGTC CGAGAACTCA CGCCAGGTCA GCCAGTCACG GAGGAGTATT GGCGGATTGT 
CTGACGAACA CTGGATACCA CGCACAGACG GGATCGTATC TTGGTCCCCT GTCAGCGATA 
GGAAGTGCCA GACCGTCAGC CTTGAAGAAA CCCGGTCACC AAAGGGCATT CAGTCAAGGT 
CAGGTAATCG AGGTACAGGG TCATTCAGTC ATGGGACACA GCCGAGTTGG CTCCAGAACG 
GACTTTATTC TTCCGCCTGG GCACAGAGAG GAATCCAGGC CACCCACTGC TGGAAAGGTG 
CCCTCCTTTC GAGGTCACTC GCGACAAGCG TCCAGATCAG AGTCGATATA CACGATAAGA 
CGTAGCGAAG AACCTCCTTG GTGGAGAAAA CTTTGGGCTC GATGTTTCGG TCCGTTACCC 
GATGAACCTC GCTTAAGGAC AATAGTACCA AATCATTTAA TATCGCCGAA AACACCTCCG 
AGTCAGCATC CTAATGGCAA AAGAGTGAAC AATAGAGTAC GCACAACAAA GTACACCACG 
CTGAGCTTCC TACCTCGCAA TCTTCTCGAA CAATTTCACC GTGTGGCCAA TATATATTTT 
GTGTTTATTG TACTGCTTAA TTGGGTGCCA GCTATAAACG CGTTCGGCAA AGAAGTAGCC 
ATGATACCTG TAATCTTCGT GCTGGGCGTG ACTGCACTAA AAGATTACTT CGAGGATCGC 
CGAAGACTGG CCAGCGATCG ACGCGTCAAT AATTCCACTT GTCGAGTTTA CGTCGGGGAG 
GACGACAGGT ATGCAAAGGT GGCGTGGAAA GATGTAAAGG TCGGTGACCT AGTTCATCTC 
TCCAACAACG AACTCGTGCC AGCCGATGTG TTGCTGTTAC GAAGCAGCGA TCCTCAGGGC 
ATTGCTTACA TCGACACGTG TAATCTCGAC GGTGAAACGA ATCTGAAACA ACGACAAGTC 
GTGAGAGGCT TCGTGGATTT TCAGGACACT TTTCAACCCG CCAAATTCCG ATCAGTAATC 
GAAGTCGATC AGCCGAGCAC CAGAATATAC AGGTTTCATG GAGCCGTCGT TCATCCGAAC 
GGAGGTAGAG TACCTGTTTC GACGGAGAAT CTTTTGCTCA GGGAGTGTTT GTTAAAGAAC 
ACCGATTTTG TCGAGGGAAT CGTGATATAC GCCGGTCACG AAACGAAAGC CTTGCTCAAT 
AACGGTGGAC CTCGGTACAA GTGTTCTCGC CTCGAGAAAC AAATGAACAG AGATGTCGTG 
TGGTGTGTGG TGATTTTGGT GGTGCTGTGC CTGATCGGTG CAATCGGTTG CCGATTTTGG 
CTCTCCAACT ATTCCGGTTT GTCGTTCGTA CCGTTTATTC CCGTTCTACA AGACCCAAAC 
TACGAGAGCA TGCTAACGTT TTGGACGTTC GTGATCATCC TTCAAGTGAT GATACCCTTG 
AGTCTATACG TCACTCTAGA AATGGCGAAA GTCGGCCAGG TTTATCACAT TGGTCACGAT 
GTCGCCCTGT ACGATGCGGA GACGGGACGC TCGGCTGAAT GTCGCGCGCT CAATATCACG 
GAGGAATTAG GTCAGGTGCA GTACATATTT TCTGATAAGA CGGGTACGCT GACGGAAAAC 
AAAATGCTGT TCAGACGATG TGTCGTGGGG GGTCAGGATT ATTCGCACAC GGGGGACGGT 
GAGAATTTGG TACCTTCCTC GCGGCTCAAG GAAGATCTCC TTATAGGTAC ATTTAGACAG 
CATCTACAAG AATTCCTAGT CGTATTAGCG ATATGTAATA CCGTTGTGGT CAATTCTCAA 
CCTCATTACG ACACTATGAA CTCTAGTGGA GTGATCGAGG ACCCTCAGCG GAACGGCGAT 
GAAACTGGAA GGTACACGAG AATGATAGAC TCAAGAAGTT TAACGCCTTC GCCAGTTATC 
CCTTTGTCCA CGCTCTCGCA TCCCACGGAT AGCCTCGACG AAAACGTGTC GTCGATTTCA 
TCGATGGTTG AAATCGAATC TGTTTTAAAT AGCACCTCGA AACTATCGAG TAAACTGCCT 
AGGCCAAAGT TTCTGAATGT GACGACGATA CCTAGTTTAG GATTGTTTGG AAGAAAATTG 
TCTCCGAACG GAGCGCAGAA AAGACAGGGT CAGCCGAGTC CCGTTACCGA TACGAACGGC 
AAGAACGGAG GCGAGCTGTT ACCTACCGCT GCGATTTACG AAGCGGAAAG CCCGGACGAG 
CTCGCCTTGG TGAACGCTGC TTGCGCTTAT GACGTGAAGC TTCTTAAACG AACAGCACGT 
AGCGCGATTG TCTGTTTGCC AGATAAATCT ATCCTTACTT TTGAGATTCT TCATGTTTTA 
CCATTCGATT CTAATAGAAA ATGTATGTCC ATCTTAGTAC GACATCCTCT GACGAACGAG 
CTTGTCTTGT ATAGCAAGGG TGCCGACACG TCTATACTGT CATCCCTAGT TTCTCAAGAA 
GAGAATTCCA TAACGACGAT TAAAGCTCGC CAGCATTTGC AATCTTATGC ACGTCAAGGT 
CTCCGAACCT TAGTGATGGC TAAAAAATCC CTGACTGCTC AGGAATACGA GACTTGGCGA 
CAAGCTCATA CAGAGGCGGA ATTGGCAATG GAAAATCGGG AATTGCGTAT CAAAAATTCG 
TACGCATCTC TTGAATCGCA TTTAACGCTT CTAGGTGCTA CTGGAATCGA AGACAAACTT 
CAAGCAGGTG TGCCTGAAAC AATGGCTACC CTAATGGCAG CAGGGATTGT AATTTGGGTG 
TTAACAGGAG ATAAACCAGA GACAGCAGTT AACATTGCAT ATTCAGCGCG TCTCTTTTCG 
CCAGCGATGC AATTATTGTG GTTGCAAGCA AGGTCCAAAA CCGTTGCAGA AACCTTGATC 
CGTGGATATT TAGAATCTGT TCGAAGAGAA AGTACGGCTC ACAGCAATGC ACGAAGCGGG 
TCGAATAACA GTAGAGATAT TACGATGCTT AATAATCGTG ATCCCGCGGA GAATGATGCT 
CATCGAATAG ACAGTCCATG GCCAAGACAA CGTGCACTCG TTGTAGATGG CAAGACACTG 
ACCGTTATTC TTGATCCACG ATCAGGATTG ATCCAACCAT TTCTTGAATT GACTCAAATG 
TGCTCCAGTG TGCTAGCATG TCGAGCAACG CCTCTTCAGA AGGCGTACAT AGTGAGGGTA 
GTAAAGGAAC AACTGGGAAT GAGAACTCTA GCAATTGGTG ATGGTGCGAA CGACGTTAGT 
ATGATTCAAA CAGCTGATGT GGGAGTGGGT ATCTCAGGAC ACGAAGTTGT ACTGTGGCTT 
TACTGGATCA GTTATGATGG ACCAAATTTA TCTGATGTTG TACAATTTGC TGTTCACATC 
CATGCCACCA CTCATATTGG GTGTGTACGA CCGAATAGCT TCACCCGGTG TTTTGATGTC 
TATGCCTCAT CTCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012136584.1
CDS	544..4221
Translation	MSEAGAQEAASEIERQASVYVFPGPGPNHQSYPGSAYASMQPPTALIPDVSIANTTPLPATFGKFAKTEVDGNEAPGNRMRPCPRTHARSASHGGVLADCLTNTGYHAQTGSYLGPLSAIGSARPSALKKPGHQRAFSQGQVIEVQGHSVMGHSRVGSRTDFILPPGHREESRPPTAGKVPSFRGHSRQASRSESIYTIRRSEEPPWWRKLWARCFGPLPDEPRLRTIVPNHLISPKTPPSQHPNGKRVNNRVRTTKYTTLSFLPRNLLEQFHRVANIYFVFIVLLNWVPAINAFGKEVAMIPVIFVLGVTALKDYFEDRRRLASDRRVNNSTCRVYVGEDDRYAKVAWKDVKVGDLVHLSNNELVPADVLLLRSSDPQGIAYIDTCNLDGETNLKQRQVVRGFVDFQDTFQPAKFRSVIEVDQPSTRIYRFHGAVVHPNGGRVPVSTENLLLRECLLKNTDFVEGIVIYAGHETKALLNNGGPRYKCSRLEKQMNRDVVWCVVILVVLCLIGAIGCRFWLSNYSGLSFVPFIPVLQDPNYESMLTFWTFVIILQVMIPLSLYVTLEMAKVGQVYHIGHDVALYDAETGRSAECRALNITEELGQVQYIFSDKTGTLTENKMLFRRCVVGGQDYSHTGDGENLVPSSRLKEDLLIGTFRQHLQEFLVVLAICNTVVVNSQPHYDTMNSSGVIEDPQRNGDETGRYTRMIDSRSLTPSPVIPLSTLSHPTDSLDENVSSISSMVEIESVLNSTSKLSSKLPRPKFLNVTTIPSLGLFGRKLSPNGAQKRQGQPSPVTDTNGKNGGELLPTAAIYEAESPDELALVNAACAYDVKLLKRTARSAIVCLPDKSILTFEILHVLPFDSNRKCMSILVRHPLTNELVLYSKGADTSILSSLVSQEENSITTIKARQHLQSYARQGLRTLVMAKKSLTAQEYETWRQAHTEAELAMENRELRIKNSYASLESHLTLLGATGIEDKLQAGVPETMATLMAAGIVIWVLTGDKPETAVNIAYSARLFSPAMQLLWLQARSKTVAETLIRGYLESVRRESTAHSNARSGSNNSRDITMLNNRDPAENDAHRIDSPWPRQRALVVDGKTLTVILDPRSGLIQPFLELTQMCSSVLACRATPLQKAYIVRVVKEQLGMRTLAIGDGANDVSMIQTADVGVGISGHEVVLWLYWISYDGPNLSDVVQFAVHIHATTHIGCVRPNSFTRCFDVYASSL

Target ORF information:

RefSeq Version	XM_012281194.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata probable phospholipid-transporting ATPase VA (LOC100874615), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012281194.1

ORF Insert Sequence:

ATGTCCGAAG CCGGCGCGCA AGAAGCTGCG AGCGAGATCG AGAGGCAGGC GTCGGTTTAC 
GTGTTCCCAG GACCGGGACC GAATCACCAG TCCTATCCCG GTTCGGCGTA CGCGTCGATG 
CAACCACCGA CCGCCTTGAT TCCCGATGTC TCGATCGCGA ACACGACCCC TTTGCCCGCA 
ACGTTCGGCA AGTTCGCGAA GACCGAGGTC GACGGGAACG AGGCGCCAGG GAACAGAATG 
AGACCGTGTC CGAGAACTCA CGCCAGGTCA GCCAGTCACG GAGGAGTATT GGCGGATTGT 
CTGACGAACA CTGGATACCA CGCACAGACG GGATCGTATC TTGGTCCCCT GTCAGCGATA 
GGAAGTGCCA GACCGTCAGC CTTGAAGAAA CCCGGTCACC AAAGGGCATT CAGTCAAGGT 
CAGGTAATCG AGGTACAGGG TCATTCAGTC ATGGGACACA GCCGAGTTGG CTCCAGAACG 
GACTTTATTC TTCCGCCTGG GCACAGAGAG GAATCCAGGC CACCCACTGC TGGAAAGGTG 
CCCTCCTTTC GAGGTCACTC GCGACAAGCG TCCAGATCAG AGTCGATATA CACGATAAGA 
CGTAGCGAAG AACCTCCTTG GTGGAGAAAA CTTTGGGCTC GATGTTTCGG TCCGTTACCC 
GATGAACCTC GCTTAAGGAC AATAGTACCA AATCATTTAA TATCGCCGAA AACACCTCCG 
AGTCAGCATC CTAATGGCAA AAGAGTGAAC AATAGAGTAC GCACAACAAA GTACACCACG 
CTGAGCTTCC TACCTCGCAA TCTTCTCGAA CAATTTCACC GTGTGGCCAA TATATATTTT 
GTGTTTATTG TACTGCTTAA TTGGGTGCCA GCTATAAACG CGTTCGGCAA AGAAGTAGCC 
ATGATACCTG TAATCTTCGT GCTGGGCGTG ACTGCACTAA AAGATTACTT CGAGGATCGC 
CGAAGACTGG CCAGCGATCG ACGCGTCAAT AATTCCACTT GTCGAGTTTA CGTCGGGGAG 
GACGACAGGT ATGCAAAGGT GGCGTGGAAA GATGTAAAGG TCGGTGACCT AGTTCATCTC 
TCCAACAACG AACTCGTGCC AGCCGATGTG TTGCTGTTAC GAAGCAGCGA TCCTCAGGGC 
ATTGCTTACA TCGACACGTG TAATCTCGAC GGTGAAACGA ATCTGAAACA ACGACAAGTC 
GTGAGAGGCT TCGTGGATTT TCAGGACACT TTTCAACCCG CCAAATTCCG ATCAGTAATC 
GAAGTCGATC AGCCGAGCAC CAGAATATAC AGGTTTCATG GAGCCGTCGT TCATCCGAAC 
GGAGGTAGAG TACCTGTTTC GACGGAGAAT CTTTTGCTCA GGGAGTGTTT GTTAAAGAAC 
ACCGATTTTG TCGAGGGAAT CGTGATATAC GCCGGTCACG AAACGAAAGC CTTGCTCAAT 
AACGGTGGAC CTCGGTACAA GTGTTCTCGC CTCGAGAAAC AAATGAACAG AGATGTCGTG 
TGGTGTGTGG TGATTTTGGT GGTGCTGTGC CTGATCGGTG CAATCGGTTG CCGATTTTGG 
CTCTCCAACT ATTCCGGTTT GTCGTTCGTA CCGTTTATTC CCGTTCTACA AGACCCAAAC 
TACGAGAGCA TGCTAACGTT TTGGACGTTC GTGATCATCC TTCAAGTGAT GATACCCTTG 
AGTCTATACG TCACTCTAGA AATGGCGAAA GTCGGCCAGG TTTATCACAT TGGTCACGAT 
GTCGCCCTGT ACGATGCGGA GACGGGACGC TCGGCTGAAT GTCGCGCGCT CAATATCACG 
GAGGAATTAG GTCAGGTGCA GTACATATTT TCTGATAAGA CGGGTACGCT GACGGAAAAC 
AAAATGCTGT TCAGACGATG TGTCGTGGGG GGTCAGGATT ATTCGCACAC GGGGGACGGT 
GAGAATTTGG TACCTTCCTC GCGGCTCAAG GAAGATCTCC TTATAGGTAC ATTTAGACAG 
CATCTACAAG AATTCCTAGT CGTATTAGCG ATATGTAATA CCGTTGTGGT CAATTCTCAA 
CCTCATTACG ACACTATGAA CTCTAGTGGA GTGATCGAGG ACCCTCAGCG GAACGGCGAT 
GAAACTGGAA GGTACACGAG AATGATAGAC TCAAGAAGTT TAACGCCTTC GCCAGTTATC 
CCTTTGTCCA CGCTCTCGCA TCCCACGGAT AGCCTCGACG AAAACGTGTC GTCGATTTCA 
TCGATGGTTG AAATCGAATC TGTTTTAAAT AGCACCTCGA AACTATCGAG TAAACTGCCT 
AGGCCAAAGT TTCTGAATGT GACGACGATA CCTAGTTTAG GATTGTTTGG AAGAAAATTG 
TCTCCGAACG GAGCGCAGAA AAGACAGGGT CAGCCGAGTC CCGTTACCGA TACGAACGGC 
AAGAACGGAG GCGAGCTGTT ACCTACCGCT GCGATTTACG AAGCGGAAAG CCCGGACGAG 
CTCGCCTTGG TGAACGCTGC TTGCGCTTAT GACGTGAAGC TTCTTAAACG AACAGCACGT 
AGCGCGATTG TCTGTTTGCC AGATAAATCT ATCCTTACTT TTGAGATTCT TCATGTTTTA 
CCATTCGATT CTAATAGAAA ATGTATGTCC ATCTTAGTAC GACATCCTCT GACGAACGAG 
CTTGTCTTGT ATAGCAAGGG TGCCGACACG TCTATACTGT CATCCCTAGT TTCTCAAGAA 
GAGAATTCCA TAACGACGAT TAAAGCTCGC CAGCATTTGC AATCTTATGC ACGTCAAGGT 
CTCCGAACCT TAGTGATGGC TAAAAAATCC CTGACTGCTC AGGAATACGA GACTTGGCGA 
CAAGCTCATA CAGAGGCGGA ATTGGCAATG GAAAATCGGG AATTGCGTAT CAAAAATTCG 
TACGCATCTC TTGAATCGCA TTTAACGCTT CTAGGTGCTA CTGGAATCGA AGACAAACTT 
CAAGCAGGTG TGCCTGAAAC AATGGCTACC CTAATGGCAG CAGGGATTGT AATTTGGGTG 
TTAACAGGAG ATAAACCAGA GACAGCAGTT AACATTGCAT ATTCAGCGCG TCTCTTTTCG 
CCAGCGATGC AATTATTGTG GTTGCAAGCA AGGTCCAAAA CCGTTGCAGA AACCTTGATC 
CGTGGATATT TAGAATCTGT TCGAAGAGAA AGTACGGCTC ACAGCAATGC ACGAAGCGGG 
TCGAATAACA GTAGAGATAT TACGATGCTT AATAATCGTG ATCCCGCGGA GAATGATGCT 
CATCGAATAG ACAGTCCATG GCCAAGACAA CGTGCACTCG TTGTAGATGG CAAGACACTG 
ACCGTTATTC TTGATCCACG ATCAGGATTG ATCCAACCAT TTCTTGAATT GACTCAAATG 
TGCTCCAGTG TGCTAGCATG TCGAGCAACG CCTCTTCAGA AGGCGTACAT AGTGAGGGTA 
GTAAAGGAAC AACTGGGAAT GAGAACTCTA GCAATTGGTG ATGGTGCGAA CGACGTTAGT 
ATGATTCAAA CAGCTGATGT GGGAGTGGGT ATCTCAGGAC ACGAAGTTGT ACTGTGGCTT 
TACTGGATCA GTTATGATGG ACCAAATTTA TCTGATGTTG TACAATTTGC TGTTCACATC 
CATGCCACCA CTCATATTGG GTGTGTACGA CCGAATAGCT TCACCCGGTG TTTTGATGTC 
TATGCCTCAT CTCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100874615 ( XM_012281195.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012136585.1 Megachile rotundata probable phospholipid-transporting ATPase VA (LOC100874615), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMe101060
Clone ID Related Accession (Same CDS sequence)	XM_012281194.1 , XM_012281195.1
Accession Version	XM_012281195.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	probable phospholipid-transporting ATPase VA isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797118.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGAAG CCGGCGCGCA AGAAGCTGCG AGCGAGATCG AGAGGCAGGC GTCGGTTTAC 
GTGTTCCCAG GACCGGGACC GAATCACCAG TCCTATCCCG GTTCGGCGTA CGCGTCGATG 
CAACCACCGA CCGCCTTGAT TCCCGATGTC TCGATCGCGA ACACGACCCC TTTGCCCGCA 
ACGTTCGGCA AGTTCGCGAA GACCGAGGTC GACGGGAACG AGGCGCCAGG GAACAGAATG 
AGACCGTGTC CGAGAACTCA CGCCAGGTCA GCCAGTCACG GAGGAGTATT GGCGGATTGT 
CTGACGAACA CTGGATACCA CGCACAGACG GGATCGTATC TTGGTCCCCT GTCAGCGATA 
GGAAGTGCCA GACCGTCAGC CTTGAAGAAA CCCGGTCACC AAAGGGCATT CAGTCAAGGT 
CAGGTAATCG AGGTACAGGG TCATTCAGTC ATGGGACACA GCCGAGTTGG CTCCAGAACG 
GACTTTATTC TTCCGCCTGG GCACAGAGAG GAATCCAGGC CACCCACTGC TGGAAAGGTG 
CCCTCCTTTC GAGGTCACTC GCGACAAGCG TCCAGATCAG AGTCGATATA CACGATAAGA 
CGTAGCGAAG AACCTCCTTG GTGGAGAAAA CTTTGGGCTC GATGTTTCGG TCCGTTACCC 
GATGAACCTC GCTTAAGGAC AATAGTACCA AATCATTTAA TATCGCCGAA AACACCTCCG 
AGTCAGCATC CTAATGGCAA AAGAGTGAAC AATAGAGTAC GCACAACAAA GTACACCACG 
CTGAGCTTCC TACCTCGCAA TCTTCTCGAA CAATTTCACC GTGTGGCCAA TATATATTTT 
GTGTTTATTG TACTGCTTAA TTGGGTGCCA GCTATAAACG CGTTCGGCAA AGAAGTAGCC 
ATGATACCTG TAATCTTCGT GCTGGGCGTG ACTGCACTAA AAGATTACTT CGAGGATCGC 
CGAAGACTGG CCAGCGATCG ACGCGTCAAT AATTCCACTT GTCGAGTTTA CGTCGGGGAG 
GACGACAGGT ATGCAAAGGT GGCGTGGAAA GATGTAAAGG TCGGTGACCT AGTTCATCTC 
TCCAACAACG AACTCGTGCC AGCCGATGTG TTGCTGTTAC GAAGCAGCGA TCCTCAGGGC 
ATTGCTTACA TCGACACGTG TAATCTCGAC GGTGAAACGA ATCTGAAACA ACGACAAGTC 
GTGAGAGGCT TCGTGGATTT TCAGGACACT TTTCAACCCG CCAAATTCCG ATCAGTAATC 
GAAGTCGATC AGCCGAGCAC CAGAATATAC AGGTTTCATG GAGCCGTCGT TCATCCGAAC 
GGAGGTAGAG TACCTGTTTC GACGGAGAAT CTTTTGCTCA GGGAGTGTTT GTTAAAGAAC 
ACCGATTTTG TCGAGGGAAT CGTGATATAC GCCGGTCACG AAACGAAAGC CTTGCTCAAT 
AACGGTGGAC CTCGGTACAA GTGTTCTCGC CTCGAGAAAC AAATGAACAG AGATGTCGTG 
TGGTGTGTGG TGATTTTGGT GGTGCTGTGC CTGATCGGTG CAATCGGTTG CCGATTTTGG 
CTCTCCAACT ATTCCGGTTT GTCGTTCGTA CCGTTTATTC CCGTTCTACA AGACCCAAAC 
TACGAGAGCA TGCTAACGTT TTGGACGTTC GTGATCATCC TTCAAGTGAT GATACCCTTG 
AGTCTATACG TCACTCTAGA AATGGCGAAA GTCGGCCAGG TTTATCACAT TGGTCACGAT 
GTCGCCCTGT ACGATGCGGA GACGGGACGC TCGGCTGAAT GTCGCGCGCT CAATATCACG 
GAGGAATTAG GTCAGGTGCA GTACATATTT TCTGATAAGA CGGGTACGCT GACGGAAAAC 
AAAATGCTGT TCAGACGATG TGTCGTGGGG GGTCAGGATT ATTCGCACAC GGGGGACGGT 
GAGAATTTGG TACCTTCCTC GCGGCTCAAG GAAGATCTCC TTATAGGTAC ATTTAGACAG 
CATCTACAAG AATTCCTAGT CGTATTAGCG ATATGTAATA CCGTTGTGGT CAATTCTCAA 
CCTCATTACG ACACTATGAA CTCTAGTGGA GTGATCGAGG ACCCTCAGCG GAACGGCGAT 
GAAACTGGAA GGTACACGAG AATGATAGAC TCAAGAAGTT TAACGCCTTC GCCAGTTATC 
CCTTTGTCCA CGCTCTCGCA TCCCACGGAT AGCCTCGACG AAAACGTGTC GTCGATTTCA 
TCGATGGTTG AAATCGAATC TGTTTTAAAT AGCACCTCGA AACTATCGAG TAAACTGCCT 
AGGCCAAAGT TTCTGAATGT GACGACGATA CCTAGTTTAG GATTGTTTGG AAGAAAATTG 
TCTCCGAACG GAGCGCAGAA AAGACAGGGT CAGCCGAGTC CCGTTACCGA TACGAACGGC 
AAGAACGGAG GCGAGCTGTT ACCTACCGCT GCGATTTACG AAGCGGAAAG CCCGGACGAG 
CTCGCCTTGG TGAACGCTGC TTGCGCTTAT GACGTGAAGC TTCTTAAACG AACAGCACGT 
AGCGCGATTG TCTGTTTGCC AGATAAATCT ATCCTTACTT TTGAGATTCT TCATGTTTTA 
CCATTCGATT CTAATAGAAA ATGTATGTCC ATCTTAGTAC GACATCCTCT GACGAACGAG 
CTTGTCTTGT ATAGCAAGGG TGCCGACACG TCTATACTGT CATCCCTAGT TTCTCAAGAA 
GAGAATTCCA TAACGACGAT TAAAGCTCGC CAGCATTTGC AATCTTATGC ACGTCAAGGT 
CTCCGAACCT TAGTGATGGC TAAAAAATCC CTGACTGCTC AGGAATACGA GACTTGGCGA 
CAAGCTCATA CAGAGGCGGA ATTGGCAATG GAAAATCGGG AATTGCGTAT CAAAAATTCG 
TACGCATCTC TTGAATCGCA TTTAACGCTT CTAGGTGCTA CTGGAATCGA AGACAAACTT 
CAAGCAGGTG TGCCTGAAAC AATGGCTACC CTAATGGCAG CAGGGATTGT AATTTGGGTG 
TTAACAGGAG ATAAACCAGA GACAGCAGTT AACATTGCAT ATTCAGCGCG TCTCTTTTCG 
CCAGCGATGC AATTATTGTG GTTGCAAGCA AGGTCCAAAA CCGTTGCAGA AACCTTGATC 
CGTGGATATT TAGAATCTGT TCGAAGAGAA AGTACGGCTC ACAGCAATGC ACGAAGCGGG 
TCGAATAACA GTAGAGATAT TACGATGCTT AATAATCGTG ATCCCGCGGA GAATGATGCT 
CATCGAATAG ACAGTCCATG GCCAAGACAA CGTGCACTCG TTGTAGATGG CAAGACACTG 
ACCGTTATTC TTGATCCACG ATCAGGATTG ATCCAACCAT TTCTTGAATT GACTCAAATG 
TGCTCCAGTG TGCTAGCATG TCGAGCAACG CCTCTTCAGA AGGCGTACAT AGTGAGGGTA 
GTAAAGGAAC AACTGGGAAT GAGAACTCTA GCAATTGGTG ATGGTGCGAA CGACGTTAGT 
ATGATTCAAA CAGCTGATGT GGGAGTGGGT ATCTCAGGAC ACGAAGTTGT ACTGTGGCTT 
TACTGGATCA GTTATGATGG ACCAAATTTA TCTGATGTTG TACAATTTGC TGTTCACATC 
CATGCCACCA CTCATATTGG GTGTGTACGA CCGAATAGCT TCACCCGGTG TTTTGATGTC 
TATGCCTCAT CTCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012136585.1
CDS	544..4221
Translation	MSEAGAQEAASEIERQASVYVFPGPGPNHQSYPGSAYASMQPPTALIPDVSIANTTPLPATFGKFAKTEVDGNEAPGNRMRPCPRTHARSASHGGVLADCLTNTGYHAQTGSYLGPLSAIGSARPSALKKPGHQRAFSQGQVIEVQGHSVMGHSRVGSRTDFILPPGHREESRPPTAGKVPSFRGHSRQASRSESIYTIRRSEEPPWWRKLWARCFGPLPDEPRLRTIVPNHLISPKTPPSQHPNGKRVNNRVRTTKYTTLSFLPRNLLEQFHRVANIYFVFIVLLNWVPAINAFGKEVAMIPVIFVLGVTALKDYFEDRRRLASDRRVNNSTCRVYVGEDDRYAKVAWKDVKVGDLVHLSNNELVPADVLLLRSSDPQGIAYIDTCNLDGETNLKQRQVVRGFVDFQDTFQPAKFRSVIEVDQPSTRIYRFHGAVVHPNGGRVPVSTENLLLRECLLKNTDFVEGIVIYAGHETKALLNNGGPRYKCSRLEKQMNRDVVWCVVILVVLCLIGAIGCRFWLSNYSGLSFVPFIPVLQDPNYESMLTFWTFVIILQVMIPLSLYVTLEMAKVGQVYHIGHDVALYDAETGRSAECRALNITEELGQVQYIFSDKTGTLTENKMLFRRCVVGGQDYSHTGDGENLVPSSRLKEDLLIGTFRQHLQEFLVVLAICNTVVVNSQPHYDTMNSSGVIEDPQRNGDETGRYTRMIDSRSLTPSPVIPLSTLSHPTDSLDENVSSISSMVEIESVLNSTSKLSSKLPRPKFLNVTTIPSLGLFGRKLSPNGAQKRQGQPSPVTDTNGKNGGELLPTAAIYEAESPDELALVNAACAYDVKLLKRTARSAIVCLPDKSILTFEILHVLPFDSNRKCMSILVRHPLTNELVLYSKGADTSILSSLVSQEENSITTIKARQHLQSYARQGLRTLVMAKKSLTAQEYETWRQAHTEAELAMENRELRIKNSYASLESHLTLLGATGIEDKLQAGVPETMATLMAAGIVIWVLTGDKPETAVNIAYSARLFSPAMQLLWLQARSKTVAETLIRGYLESVRRESTAHSNARSGSNNSRDITMLNNRDPAENDAHRIDSPWPRQRALVVDGKTLTVILDPRSGLIQPFLELTQMCSSVLACRATPLQKAYIVRVVKEQLGMRTLAIGDGANDVSMIQTADVGVGISGHEVVLWLYWISYDGPNLSDVVQFAVHIHATTHIGCVRPNSFTRCFDVYASSL

Target ORF information:

RefSeq Version	XM_012281195.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata probable phospholipid-transporting ATPase VA (LOC100874615), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012281195.1

ORF Insert Sequence:

ATGTCCGAAG CCGGCGCGCA AGAAGCTGCG AGCGAGATCG AGAGGCAGGC GTCGGTTTAC 
GTGTTCCCAG GACCGGGACC GAATCACCAG TCCTATCCCG GTTCGGCGTA CGCGTCGATG 
CAACCACCGA CCGCCTTGAT TCCCGATGTC TCGATCGCGA ACACGACCCC TTTGCCCGCA 
ACGTTCGGCA AGTTCGCGAA GACCGAGGTC GACGGGAACG AGGCGCCAGG GAACAGAATG 
AGACCGTGTC CGAGAACTCA CGCCAGGTCA GCCAGTCACG GAGGAGTATT GGCGGATTGT 
CTGACGAACA CTGGATACCA CGCACAGACG GGATCGTATC TTGGTCCCCT GTCAGCGATA 
GGAAGTGCCA GACCGTCAGC CTTGAAGAAA CCCGGTCACC AAAGGGCATT CAGTCAAGGT 
CAGGTAATCG AGGTACAGGG TCATTCAGTC ATGGGACACA GCCGAGTTGG CTCCAGAACG 
GACTTTATTC TTCCGCCTGG GCACAGAGAG GAATCCAGGC CACCCACTGC TGGAAAGGTG 
CCCTCCTTTC GAGGTCACTC GCGACAAGCG TCCAGATCAG AGTCGATATA CACGATAAGA 
CGTAGCGAAG AACCTCCTTG GTGGAGAAAA CTTTGGGCTC GATGTTTCGG TCCGTTACCC 
GATGAACCTC GCTTAAGGAC AATAGTACCA AATCATTTAA TATCGCCGAA AACACCTCCG 
AGTCAGCATC CTAATGGCAA AAGAGTGAAC AATAGAGTAC GCACAACAAA GTACACCACG 
CTGAGCTTCC TACCTCGCAA TCTTCTCGAA CAATTTCACC GTGTGGCCAA TATATATTTT 
GTGTTTATTG TACTGCTTAA TTGGGTGCCA GCTATAAACG CGTTCGGCAA AGAAGTAGCC 
ATGATACCTG TAATCTTCGT GCTGGGCGTG ACTGCACTAA AAGATTACTT CGAGGATCGC 
CGAAGACTGG CCAGCGATCG ACGCGTCAAT AATTCCACTT GTCGAGTTTA CGTCGGGGAG 
GACGACAGGT ATGCAAAGGT GGCGTGGAAA GATGTAAAGG TCGGTGACCT AGTTCATCTC 
TCCAACAACG AACTCGTGCC AGCCGATGTG TTGCTGTTAC GAAGCAGCGA TCCTCAGGGC 
ATTGCTTACA TCGACACGTG TAATCTCGAC GGTGAAACGA ATCTGAAACA ACGACAAGTC 
GTGAGAGGCT TCGTGGATTT TCAGGACACT TTTCAACCCG CCAAATTCCG ATCAGTAATC 
GAAGTCGATC AGCCGAGCAC CAGAATATAC AGGTTTCATG GAGCCGTCGT TCATCCGAAC 
GGAGGTAGAG TACCTGTTTC GACGGAGAAT CTTTTGCTCA GGGAGTGTTT GTTAAAGAAC 
ACCGATTTTG TCGAGGGAAT CGTGATATAC GCCGGTCACG AAACGAAAGC CTTGCTCAAT 
AACGGTGGAC CTCGGTACAA GTGTTCTCGC CTCGAGAAAC AAATGAACAG AGATGTCGTG 
TGGTGTGTGG TGATTTTGGT GGTGCTGTGC CTGATCGGTG CAATCGGTTG CCGATTTTGG 
CTCTCCAACT ATTCCGGTTT GTCGTTCGTA CCGTTTATTC CCGTTCTACA AGACCCAAAC 
TACGAGAGCA TGCTAACGTT TTGGACGTTC GTGATCATCC TTCAAGTGAT GATACCCTTG 
AGTCTATACG TCACTCTAGA AATGGCGAAA GTCGGCCAGG TTTATCACAT TGGTCACGAT 
GTCGCCCTGT ACGATGCGGA GACGGGACGC TCGGCTGAAT GTCGCGCGCT CAATATCACG 
GAGGAATTAG GTCAGGTGCA GTACATATTT TCTGATAAGA CGGGTACGCT GACGGAAAAC 
AAAATGCTGT TCAGACGATG TGTCGTGGGG GGTCAGGATT ATTCGCACAC GGGGGACGGT 
GAGAATTTGG TACCTTCCTC GCGGCTCAAG GAAGATCTCC TTATAGGTAC ATTTAGACAG 
CATCTACAAG AATTCCTAGT CGTATTAGCG ATATGTAATA CCGTTGTGGT CAATTCTCAA 
CCTCATTACG ACACTATGAA CTCTAGTGGA GTGATCGAGG ACCCTCAGCG GAACGGCGAT 
GAAACTGGAA GGTACACGAG AATGATAGAC TCAAGAAGTT TAACGCCTTC GCCAGTTATC 
CCTTTGTCCA CGCTCTCGCA TCCCACGGAT AGCCTCGACG AAAACGTGTC GTCGATTTCA 
TCGATGGTTG AAATCGAATC TGTTTTAAAT AGCACCTCGA AACTATCGAG TAAACTGCCT 
AGGCCAAAGT TTCTGAATGT GACGACGATA CCTAGTTTAG GATTGTTTGG AAGAAAATTG 
TCTCCGAACG GAGCGCAGAA AAGACAGGGT CAGCCGAGTC CCGTTACCGA TACGAACGGC 
AAGAACGGAG GCGAGCTGTT ACCTACCGCT GCGATTTACG AAGCGGAAAG CCCGGACGAG 
CTCGCCTTGG TGAACGCTGC TTGCGCTTAT GACGTGAAGC TTCTTAAACG AACAGCACGT 
AGCGCGATTG TCTGTTTGCC AGATAAATCT ATCCTTACTT TTGAGATTCT TCATGTTTTA 
CCATTCGATT CTAATAGAAA ATGTATGTCC ATCTTAGTAC GACATCCTCT GACGAACGAG 
CTTGTCTTGT ATAGCAAGGG TGCCGACACG TCTATACTGT CATCCCTAGT TTCTCAAGAA 
GAGAATTCCA TAACGACGAT TAAAGCTCGC CAGCATTTGC AATCTTATGC ACGTCAAGGT 
CTCCGAACCT TAGTGATGGC TAAAAAATCC CTGACTGCTC AGGAATACGA GACTTGGCGA 
CAAGCTCATA CAGAGGCGGA ATTGGCAATG GAAAATCGGG AATTGCGTAT CAAAAATTCG 
TACGCATCTC TTGAATCGCA TTTAACGCTT CTAGGTGCTA CTGGAATCGA AGACAAACTT 
CAAGCAGGTG TGCCTGAAAC AATGGCTACC CTAATGGCAG CAGGGATTGT AATTTGGGTG 
TTAACAGGAG ATAAACCAGA GACAGCAGTT AACATTGCAT ATTCAGCGCG TCTCTTTTCG 
CCAGCGATGC AATTATTGTG GTTGCAAGCA AGGTCCAAAA CCGTTGCAGA AACCTTGATC 
CGTGGATATT TAGAATCTGT TCGAAGAGAA AGTACGGCTC ACAGCAATGC ACGAAGCGGG 
TCGAATAACA GTAGAGATAT TACGATGCTT AATAATCGTG ATCCCGCGGA GAATGATGCT 
CATCGAATAG ACAGTCCATG GCCAAGACAA CGTGCACTCG TTGTAGATGG CAAGACACTG 
ACCGTTATTC TTGATCCACG ATCAGGATTG ATCCAACCAT TTCTTGAATT GACTCAAATG 
TGCTCCAGTG TGCTAGCATG TCGAGCAACG CCTCTTCAGA AGGCGTACAT AGTGAGGGTA 
GTAAAGGAAC AACTGGGAAT GAGAACTCTA GCAATTGGTG ATGGTGCGAA CGACGTTAGT 
ATGATTCAAA CAGCTGATGT GGGAGTGGGT ATCTCAGGAC ACGAAGTTGT ACTGTGGCTT 
TACTGGATCA GTTATGATGG ACCAAATTTA TCTGATGTTG TACAATTTGC TGTTCACATC 
CATGCCACCA CTCATATTGG GTGTGTACGA CCGAATAGCT TCACCCGGTG TTTTGATGTC 
TATGCCTCAT CTCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

[email protected]

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

LOC100874615 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム