LOC25501716 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC25501716
Entrez Gene ID	25501716
Full Name	RecQ family ATP-dependent DNA helicase
Gene Type	protein-coding
Organism	Medicago truncatula(barrel medic)

mRNA and Protein(s)

mRNA	Protein	Name
XM_013591073.1	XP_013446527.1	RecQ family ATP-dependent DNA helicase
XM_013591073.2	XP_013446527.1	ATP-dependent DNA helicase Q-like 5

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
Tang H, Krishnakumar V, Bidwell S, Rosen B, Chan A, Zhou S, Gentzbittel L, Childs KL, Yandell M, Gundlach H, Mayer KF, Schwartz DC, Town CD
BMC genomics15312(2014 Apr)

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Young ND, Debell? F, Oldroyd GE, Geurts R, Cannon SB, Udvardi MK, Benedito VA, Mayer KF, Gouzy J, Schoof H, Van de Peer Y, Proost S, Cook DR, Meyers BC, Spannagl M, Cheung F, De Mita S, Krishnakumar V, Gundlach H, Zhou S, Mudge J, Bharti AK, Murray JD, Naoumkina MA, Rosen B, Silverstein KA, Tang H, Rombauts S, Zhao PX, Zhou P, Barbe V, Bardou P, Bechner M, Bellec A, Berger A, Berg?s H, Bidwell S, Bisseling T, Choisne N, Couloux A, Denny R, Deshpande S, Dai X, Doyle JJ, Dudez AM, Farmer AD, Fouteau S, Franken C, Gibelin C, Gish J, Goldstein S, Gonz?lez AJ, Green PJ, Hallab A, Hartog M, Hua A, Humphray SJ, Jeong DH, Jing Y, J?cker A, Kenton SM, Kim DJ, Klee K, Lai H, Lang C, Lin S, Macmil SL, Magdelenat G, Matthews L, McCorrison J, Monaghan EL, Mun JH, Najar FZ, Nicholson C, Noirot C, O'Bleness M, Paule CR, Poulain J, Prion F, Qin B, Qu C, Retzel EF, Riddle C, Sallet E, Samain S, Samson N, Sanders I, Saurat O, Scarpelli C, Schiex T, Segurens B, Severin AJ, Sherrier DJ, Shi R, Sims S, Singer SR, Sinharoy S, Sterck L, Viollet A, Wang BB, Wang K, Wang M, Wang X, Warfsmann J, Weissenbach J, White DD, White JD, Wiley GB, Wincker P, Xing Y, Yang L, Yao Z, Ying F, Zhai J, Zhou L, Zuber A, D?nari? J, Dixon RA, May GD, Schwartz DC, Rogers J, Qu?tier F, Town CD, Roe BA
Nature480(7378)520-4(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC25501716 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC25501716 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMe166424	XM_013591073.2 Latest version!	Medicago truncatula ATP-dependent DNA helicase Q-like 5 (LOC25501716), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
OMe166424	XM_013591073.1	Medicago truncatula RecQ family ATP-dependent DNA helicase mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC25501716 ( XM_013591073.1 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_013446527.1 Medicago truncatula RecQ family ATP-dependent DNA helicase mRNA.

Price & Availability↑

CloneID	OMe166424
Clone ID Related Accession (Same CDS sequence)	XM_013591073.1 , XM_013591073.2
Accession Version	XM_013591073.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2754bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-24
Organism	Medicago truncatula(barrel medic)
Product	RecQ family ATP-dependent DNA helicase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_016414). ##Genome-Annotation-Data-START## Annotation Provider :: JCVI Annotation Version :: Mt4.0v2 Annotation Pipeline :: JCVI Eukaryotic Genome Annotation Pipeline Annotation Method :: EVidenceModeler (EVM), PASA Features Annotated :: Gene; mRNA; CDS; tRNA URL :: http://www.jcvi.org/medicago ##Genome-Annotation-Data-END##

ATGCAGTTTG ATTCCGATTC AGATTCCGAC GGTTCTCACA TTTCCTCAAC TCCACCAAGA 
CAACAACCAA ATTCACCACC ACCACCAAAA CCACCTCTTC CAGTTTCAAA CAAAAAATCC 
AAAACCAAAA CCAAAACCTC AAAATCCAAT AAACCCCTTT CTAAACCCCC CGAAATTCTT 
CCCGAACAAG AATCTTTCCC CTTCCCTTCC ATCCCCTCAC TTCCTTTCAA CATCCGACAA 
CGAACGTCTG ACGCTCCTTC AACTTCATCT TCTCACTCTA TCGAAACCCT CCCTGCCGGA 
TTCTTCTCCA AATCAATTTC ATTCTCCAAA ATTCGTAAAC CCTTACTCAA CCTAGAAACA 
TCCGAAGCCG AACCGGTTGT TGATTCTGCA AAAACTGTAG CGAAATCTGT GAAGAAACAT 
CCGAATTTGA TAGGTGCTAG TGTGGTAACG CCGATAGTGA AGGCACTAAA AGGCGCTGGA 
GAAGGAAATT TTGTGAAATT GAATCTCAAT GGTAAGAGGA AAAAGTTTTT GAATAAAGGA 
TGGAAGAAAA ATGGAAAATT TGGTAGTGGT AAAAGGTATT TTAGAAATAG TAATGGTAGT 
AATAGTGGTA GAAGTAGTAG AAGTAAACGT ATAAAATCGG AATACAAAGA AGACGATGAT 
GATGATGAGG GGGATGGTAT GGGTGTTGAG AATGCAAAGC AACAGGAAAA GAAGAATGGT 
TGGGAAAATG AATGCGAAGT GTTGGAGGAG GCTGTGTTAG CAGCAAGAGA TGATGCTTCT 
GATGAGAATT TGGTGAAGTT GTTGAAATTG ATTCATGGTT TTGATTCTTT TCGAGAGGGG 
CAGCTTGAGG CGATTAAGAA TGTGCTTGCT GGGAAATCGA CGATGCTTAT TTTGCCGACT 
GGTGCTGGGA AATCGTTGTG TTATCAATTG AGTGCGTTGA TTTTGCCTGG GATTACGCTT 
GTTGTTAGTC CTTTGGTAGC TTTGATGATT GATCAGCTTC GACAATTGCC TCCGCTCATT 
TCGGGCGCTC TTCTAAGCAG TGCTCAGACA TTTGAAGAAT CCTCTGAAAC GCTTAACCAG 
CTTCGTCAAG GCACAATAAA GGTGCTTTTT GTTTCACCAG AGCGATTCTT GAATGAAGAA 
TTTCTGTCTG TTATCTCTGG TGGGTTAGCC GTCTCACTTG TTGTTATTGA TGAAGCACAT 
TGTATATCTG AATGGTCTCA CAATTTCCGA CCTTCATTCA TGAGACTTAG AGCATCACTG 
CTTCATAAGA GTCTTAATGT AGGTTCTATC CTTGCAATGA CAGCAACTGC CACAACCACG 
ACTTTAGATG CCATTATGTC TGCCCTGGAT ATTCCTCGCA CAAATCTTAT TCAGAATGCA 
CACTTAAGGG ACAATTTGTG TTTGTCTGTG TCTTTGATAA AAAATAGAAT GAAAGACCTA 
CTGGTGCTTA TGAAGTCTTC TCCTTTTGCG GAAGTTAAAA GCATCATAGT ATACTGCAAA 
TTTCAGTCTG AAACTGATCA AATTAGCAGA TATTTGAATG ATAACAATAT CAAGGCAAAG 
AGTTATCATA GTGGTATTTC TGCAAAGGAA CGTGGCTACG TACAGGAGTT GTTTGGTTCA 
AACAAGATCA GAGTGGTTGT TGCAACTGTG GCATTTGGCA TGGGACTAGA TAAAAGAGAT 
GTTGGAGCAG TAATTCATTA CAGTTTACCT GGAAGTCTGG AAGAGTATGT ACAGGAGATA 
GGACGTGCCG GAAGGGATGG GAGGTTGTCC TATTGTCACC TATTTTATGA TGATGAGATG 
TATTTCAAAC TTCGTAGTCT AATGTACAGT GAAGGAATAG ATGAATATGC AGTGAATAAA 
TTTCTGTCTG AAGTGTTTCC TGCTGACAAA AGCTCCTGTG GGAAAATATG TTCGTTAATC 
AAAGAACCTG CTTCACGCAG GTTTGATATG AAAGAAGAGG TGATTCTCAC AGTTTTGACA 
CGCTTGGAGC TGGGTGACGT GCAGTACTTA CATTTACTTC CACAGACAAA TGCAACGTGT 
GTTCTGAATT TTCATATGAC TCCGGCTGTG TCCCTTGCCC AAAAGGTTTC TGCTATTGCT 
GCAATTCTGA AAAGATCTGA AAATAAGCAC GGACAATATA CTTTTGATAT TCCAACTGTG 
GCAAATGACA TGGGGGTCAC ACCAGTTGAA TTAACAAATC AGTTGTATGC CCTAAAGTTG 
AAGGGAGAAA TAACTTATGA AATGAAGGAC ATGGCCTACT GTTATAGGAT TCTTGAAGCC 
CCAGCAGACT TTTTTTCTCT ATCAGCAGAT ATTACACGAT GGTTATCGGA AGTTGAATGT 
TGTAAGGTAC GAAAAATGGA TGCAATGTTT AATGCTGCAT ATTTTGCAGT AAATCTATGT 
GATAAGATGA GTGGTTGCAG TAGTTCTGAT CACACTTCAT GCTTGCAAAG GATAATATTG 
GATTACTTTG CTGGAGTTGA TAATGCTGAT TTCTGCAAGA AAATTGATCA AAGCAGCCGT 
TTCCTGCGGG CAGATATAAA GGTTTTTCTA CAGAGCAATT CACATGCTCG CTTTACACCA 
AGAGCTGTTG CGAGGGTAAT GCATGGAATT GCAAGCCCAG CCTACCCTTA TACAATATGG 
GGTAAAACCC ATTTCTGGGG AAGGTATACC AAGATAGACT TTAACGTTGT GATGGAAGCA 
GCTAAGGAAG AACTTAAAAC ATTTATTGGA AAAGATTACT CTAGCAATTT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013446527.1
CDS	13..2766
Translation	MQFDSDSDSDGSHISSTPPRQQPNSPPPPKPPLPVSNKKSKTKTKTSKSNKPLSKPPEILPEQESFPFPSIPSLPFNIRQRTSDAPSTSSSHSIETLPAGFFSKSISFSKIRKPLLNLETSEAEPVVDSAKTVAKSVKKHPNLIGASVVTPIVKALKGAGEGNFVKLNLNGKRKKFLNKGWKKNGKFGSGKRYFRNSNGSNSGRSSRSKRIKSEYKEDDDDDEGDGMGVENAKQQEKKNGWENECEVLEEAVLAARDDASDENLVKLLKLIHGFDSFREGQLEAIKNVLAGKSTMLILPTGAGKSLCYQLSALILPGITLVVSPLVALMIDQLRQLPPLISGALLSSAQTFEESSETLNQLRQGTIKVLFVSPERFLNEEFLSVISGGLAVSLVVIDEAHCISEWSHNFRPSFMRLRASLLHKSLNVGSILAMTATATTTTLDAIMSALDIPRTNLIQNAHLRDNLCLSVSLIKNRMKDLLVLMKSSPFAEVKSIIVYCKFQSETDQISRYLNDNNIKAKSYHSGISAKERGYVQELFGSNKIRVVVATVAFGMGLDKRDVGAVIHYSLPGSLEEYVQEIGRAGRDGRLSYCHLFYDDEMYFKLRSLMYSEGIDEYAVNKFLSEVFPADKSSCGKICSLIKEPASRRFDMKEEVILTVLTRLELGDVQYLHLLPQTNATCVLNFHMTPAVSLAQKVSAIAAILKRSENKHGQYTFDIPTVANDMGVTPVELTNQLYALKLKGEITYEMKDMAYCYRILEAPADFFSLSADITRWLSEVECCKVRKMDAMFNAAYFAVNLCDKMSGCSSSDHTSCLQRIILDYFAGVDNADFCKKIDQSSRFLRADIKVFLQSNSHARFTPRAVARVMHGIASPAYPYTIWGKTHFWGRYTKIDFNVVMEAAKEELKTFIGKDYSSNL

Target ORF information:

RefSeq Version	XM_013591073.1
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula RecQ family ATP-dependent DNA helicase mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013591073.1

ORF Insert Sequence:

ATGCAGTTTG ATTCCGATTC AGATTCCGAC GGTTCTCACA TTTCCTCAAC TCCACCAAGA 
CAACAACCAA ATTCACCACC ACCACCAAAA CCACCTCTTC CAGTTTCAAA CAAAAAATCC 
AAAACCAAAA CCAAAACCTC AAAATCCAAT AAACCCCTTT CTAAACCCCC CGAAATTCTT 
CCCGAACAAG AATCTTTCCC CTTCCCTTCC ATCCCCTCAC TTCCTTTCAA CATCCGACAA 
CGAACGTCTG ACGCTCCTTC AACTTCATCT TCTCACTCTA TCGAAACCCT CCCTGCCGGA 
TTCTTCTCCA AATCAATTTC ATTCTCCAAA ATTCGTAAAC CCTTACTCAA CCTAGAAACA 
TCCGAAGCCG AACCGGTTGT TGATTCTGCA AAAACTGTAG CGAAATCTGT GAAGAAACAT 
CCGAATTTGA TAGGTGCTAG TGTGGTAACG CCGATAGTGA AGGCACTAAA AGGCGCTGGA 
GAAGGAAATT TTGTGAAATT GAATCTCAAT GGTAAGAGGA AAAAGTTTTT GAATAAAGGA 
TGGAAGAAAA ATGGAAAATT TGGTAGTGGT AAAAGGTATT TTAGAAATAG TAATGGTAGT 
AATAGTGGTA GAAGTAGTAG AAGTAAACGT ATAAAATCGG AATACAAAGA AGACGATGAT 
GATGATGAGG GGGATGGTAT GGGTGTTGAG AATGCAAAGC AACAGGAAAA GAAGAATGGT 
TGGGAAAATG AATGCGAAGT GTTGGAGGAG GCTGTGTTAG CAGCAAGAGA TGATGCTTCT 
GATGAGAATT TGGTGAAGTT GTTGAAATTG ATTCATGGTT TTGATTCTTT TCGAGAGGGG 
CAGCTTGAGG CGATTAAGAA TGTGCTTGCT GGGAAATCGA CGATGCTTAT TTTGCCGACT 
GGTGCTGGGA AATCGTTGTG TTATCAATTG AGTGCGTTGA TTTTGCCTGG GATTACGCTT 
GTTGTTAGTC CTTTGGTAGC TTTGATGATT GATCAGCTTC GACAATTGCC TCCGCTCATT 
TCGGGCGCTC TTCTAAGCAG TGCTCAGACA TTTGAAGAAT CCTCTGAAAC GCTTAACCAG 
CTTCGTCAAG GCACAATAAA GGTGCTTTTT GTTTCACCAG AGCGATTCTT GAATGAAGAA 
TTTCTGTCTG TTATCTCTGG TGGGTTAGCC GTCTCACTTG TTGTTATTGA TGAAGCACAT 
TGTATATCTG AATGGTCTCA CAATTTCCGA CCTTCATTCA TGAGACTTAG AGCATCACTG 
CTTCATAAGA GTCTTAATGT AGGTTCTATC CTTGCAATGA CAGCAACTGC CACAACCACG 
ACTTTAGATG CCATTATGTC TGCCCTGGAT ATTCCTCGCA CAAATCTTAT TCAGAATGCA 
CACTTAAGGG ACAATTTGTG TTTGTCTGTG TCTTTGATAA AAAATAGAAT GAAAGACCTA 
CTGGTGCTTA TGAAGTCTTC TCCTTTTGCG GAAGTTAAAA GCATCATAGT ATACTGCAAA 
TTTCAGTCTG AAACTGATCA AATTAGCAGA TATTTGAATG ATAACAATAT CAAGGCAAAG 
AGTTATCATA GTGGTATTTC TGCAAAGGAA CGTGGCTACG TACAGGAGTT GTTTGGTTCA 
AACAAGATCA GAGTGGTTGT TGCAACTGTG GCATTTGGCA TGGGACTAGA TAAAAGAGAT 
GTTGGAGCAG TAATTCATTA CAGTTTACCT GGAAGTCTGG AAGAGTATGT ACAGGAGATA 
GGACGTGCCG GAAGGGATGG GAGGTTGTCC TATTGTCACC TATTTTATGA TGATGAGATG 
TATTTCAAAC TTCGTAGTCT AATGTACAGT GAAGGAATAG ATGAATATGC AGTGAATAAA 
TTTCTGTCTG AAGTGTTTCC TGCTGACAAA AGCTCCTGTG GGAAAATATG TTCGTTAATC 
AAAGAACCTG CTTCACGCAG GTTTGATATG AAAGAAGAGG TGATTCTCAC AGTTTTGACA 
CGCTTGGAGC TGGGTGACGT GCAGTACTTA CATTTACTTC CACAGACAAA TGCAACGTGT 
GTTCTGAATT TTCATATGAC TCCGGCTGTG TCCCTTGCCC AAAAGGTTTC TGCTATTGCT 
GCAATTCTGA AAAGATCTGA AAATAAGCAC GGACAATATA CTTTTGATAT TCCAACTGTG 
GCAAATGACA TGGGGGTCAC ACCAGTTGAA TTAACAAATC AGTTGTATGC CCTAAAGTTG 
AAGGGAGAAA TAACTTATGA AATGAAGGAC ATGGCCTACT GTTATAGGAT TCTTGAAGCC 
CCAGCAGACT TTTTTTCTCT ATCAGCAGAT ATTACACGAT GGTTATCGGA AGTTGAATGT 
TGTAAGGTAC GAAAAATGGA TGCAATGTTT AATGCTGCAT ATTTTGCAGT AAATCTATGT 
GATAAGATGA GTGGTTGCAG TAGTTCTGAT CACACTTCAT GCTTGCAAAG GATAATATTG 
GATTACTTTG CTGGAGTTGA TAATGCTGAT TTCTGCAAGA AAATTGATCA AAGCAGCCGT 
TTCCTGCGGG CAGATATAAA GGTTTTTCTA CAGAGCAATT CACATGCTCG CTTTACACCA 
AGAGCTGTTG CGAGGGTAAT GCATGGAATT GCAAGCCCAG CCTACCCTTA TACAATATGG 
GGTAAAACCC ATTTCTGGGG AAGGTATACC AAGATAGACT TTAACGTTGT GATGGAAGCA 
GCTAAGGAAG AACTTAAAAC ATTTATTGGA AAAGATTACT CTAGCAATTT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC25501716 ( XM_013591073.2 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_013446527.1 Medicago truncatula ATP-dependent DNA helicase Q-like 5 (LOC25501716), mRNA.

Price & Availability↑

CloneID	OMe166424
Clone ID Related Accession (Same CDS sequence)	XM_013591073.1 , XM_013591073.2
Accession Version	XM_013591073.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2754bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-22
Organism	Medicago truncatula(barrel medic)
Product	ATP-dependent DNA helicase Q-like 5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016414.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2018 this sequence version replaced XM_013591073.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Medicago truncatula Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTTTG ATTCCGATTC AGATTCCGAC GGTTCTCACA TTTCCTCAAC TCCACCAAGA 
CAACAACCAA ATTCACCACC ACCACCAAAA CCACCTCTTC CAGTTTCAAA CAAAAAATCC 
AAAACCAAAA CCAAAACCTC AAAATCCAAT AAACCCCTTT CTAAACCCCC CGAAATTCTT 
CCCGAACAAG AATCTTTCCC CTTCCCTTCC ATCCCCTCAC TTCCTTTCAA CATCCGACAA 
CGAACGTCTG ACGCTCCTTC AACTTCATCT TCTCACTCTA TCGAAACCCT CCCTGCCGGA 
TTCTTCTCCA AATCAATTTC ATTCTCCAAA ATTCGTAAAC CCTTACTCAA CCTAGAAACA 
TCCGAAGCCG AACCGGTTGT TGATTCTGCA AAAACTGTAG CGAAATCTGT GAAGAAACAT 
CCGAATTTGA TAGGTGCTAG TGTGGTAACG CCGATAGTGA AGGCACTAAA AGGCGCTGGA 
GAAGGAAATT TTGTGAAATT GAATCTCAAT GGTAAGAGGA AAAAGTTTTT GAATAAAGGA 
TGGAAGAAAA ATGGAAAATT TGGTAGTGGT AAAAGGTATT TTAGAAATAG TAATGGTAGT 
AATAGTGGTA GAAGTAGTAG AAGTAAACGT ATAAAATCGG AATACAAAGA AGACGATGAT 
GATGATGAGG GGGATGGTAT GGGTGTTGAG AATGCAAAGC AACAGGAAAA GAAGAATGGT 
TGGGAAAATG AATGCGAAGT GTTGGAGGAG GCTGTGTTAG CAGCAAGAGA TGATGCTTCT 
GATGAGAATT TGGTGAAGTT GTTGAAATTG ATTCATGGTT TTGATTCTTT TCGAGAGGGG 
CAGCTTGAGG CGATTAAGAA TGTGCTTGCT GGGAAATCGA CGATGCTTAT TTTGCCGACT 
GGTGCTGGGA AATCGTTGTG TTATCAATTG AGTGCGTTGA TTTTGCCTGG GATTACGCTT 
GTTGTTAGTC CTTTGGTAGC TTTGATGATT GATCAGCTTC GACAATTGCC TCCGCTCATT 
TCGGGCGCTC TTCTAAGCAG TGCTCAGACA TTTGAAGAAT CCTCTGAAAC GCTTAACCAG 
CTTCGTCAAG GCACAATAAA GGTGCTTTTT GTTTCACCAG AGCGATTCTT GAATGAAGAA 
TTTCTGTCTG TTATCTCTGG TGGGTTAGCC GTCTCACTTG TTGTTATTGA TGAAGCACAT 
TGTATATCTG AATGGTCTCA CAATTTCCGA CCTTCATTCA TGAGACTTAG AGCATCACTG 
CTTCATAAGA GTCTTAATGT AGGTTCTATC CTTGCAATGA CAGCAACTGC CACAACCACG 
ACTTTAGATG CCATTATGTC TGCCCTGGAT ATTCCTCGCA CAAATCTTAT TCAGAATGCA 
CACTTAAGGG ACAATTTGTG TTTGTCTGTG TCTTTGATAA AAAATAGAAT GAAAGACCTA 
CTGGTGCTTA TGAAGTCTTC TCCTTTTGCG GAAGTTAAAA GCATCATAGT ATACTGCAAA 
TTTCAGTCTG AAACTGATCA AATTAGCAGA TATTTGAATG ATAACAATAT CAAGGCAAAG 
AGTTATCATA GTGGTATTTC TGCAAAGGAA CGTGGCTACG TACAGGAGTT GTTTGGTTCA 
AACAAGATCA GAGTGGTTGT TGCAACTGTG GCATTTGGCA TGGGACTAGA TAAAAGAGAT 
GTTGGAGCAG TAATTCATTA CAGTTTACCT GGAAGTCTGG AAGAGTATGT ACAGGAGATA 
GGACGTGCCG GAAGGGATGG GAGGTTGTCC TATTGTCACC TATTTTATGA TGATGAGATG 
TATTTCAAAC TTCGTAGTCT AATGTACAGT GAAGGAATAG ATGAATATGC AGTGAATAAA 
TTTCTGTCTG AAGTGTTTCC TGCTGACAAA AGCTCCTGTG GGAAAATATG TTCGTTAATC 
AAAGAACCTG CTTCACGCAG GTTTGATATG AAAGAAGAGG TGATTCTCAC AGTTTTGACA 
CGCTTGGAGC TGGGTGACGT GCAGTACTTA CATTTACTTC CACAGACAAA TGCAACGTGT 
GTTCTGAATT TTCATATGAC TCCGGCTGTG TCCCTTGCCC AAAAGGTTTC TGCTATTGCT 
GCAATTCTGA AAAGATCTGA AAATAAGCAC GGACAATATA CTTTTGATAT TCCAACTGTG 
GCAAATGACA TGGGGGTCAC ACCAGTTGAA TTAACAAATC AGTTGTATGC CCTAAAGTTG 
AAGGGAGAAA TAACTTATGA AATGAAGGAC ATGGCCTACT GTTATAGGAT TCTTGAAGCC 
CCAGCAGACT TTTTTTCTCT ATCAGCAGAT ATTACACGAT GGTTATCGGA AGTTGAATGT 
TGTAAGGTAC GAAAAATGGA TGCAATGTTT AATGCTGCAT ATTTTGCAGT AAATCTATGT 
GATAAGATGA GTGGTTGCAG TAGTTCTGAT CACACTTCAT GCTTGCAAAG GATAATATTG 
GATTACTTTG CTGGAGTTGA TAATGCTGAT TTCTGCAAGA AAATTGATCA AAGCAGCCGT 
TTCCTGCGGG CAGATATAAA GGTTTTTCTA CAGAGCAATT CACATGCTCG CTTTACACCA 
AGAGCTGTTG CGAGGGTAAT GCATGGAATT GCAAGCCCAG CCTACCCTTA TACAATATGG 
GGTAAAACCC ATTTCTGGGG AAGGTATACC AAGATAGACT TTAACGTTGT GATGGAAGCA 
GCTAAGGAAG AACTTAAAAC ATTTATTGGA AAAGATTACT CTAGCAATTT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013446527.1
CDS	50..2803
Translation	MQFDSDSDSDGSHISSTPPRQQPNSPPPPKPPLPVSNKKSKTKTKTSKSNKPLSKPPEILPEQESFPFPSIPSLPFNIRQRTSDAPSTSSSHSIETLPAGFFSKSISFSKIRKPLLNLETSEAEPVVDSAKTVAKSVKKHPNLIGASVVTPIVKALKGAGEGNFVKLNLNGKRKKFLNKGWKKNGKFGSGKRYFRNSNGSNSGRSSRSKRIKSEYKEDDDDDEGDGMGVENAKQQEKKNGWENECEVLEEAVLAARDDASDENLVKLLKLIHGFDSFREGQLEAIKNVLAGKSTMLILPTGAGKSLCYQLSALILPGITLVVSPLVALMIDQLRQLPPLISGALLSSAQTFEESSETLNQLRQGTIKVLFVSPERFLNEEFLSVISGGLAVSLVVIDEAHCISEWSHNFRPSFMRLRASLLHKSLNVGSILAMTATATTTTLDAIMSALDIPRTNLIQNAHLRDNLCLSVSLIKNRMKDLLVLMKSSPFAEVKSIIVYCKFQSETDQISRYLNDNNIKAKSYHSGISAKERGYVQELFGSNKIRVVVATVAFGMGLDKRDVGAVIHYSLPGSLEEYVQEIGRAGRDGRLSYCHLFYDDEMYFKLRSLMYSEGIDEYAVNKFLSEVFPADKSSCGKICSLIKEPASRRFDMKEEVILTVLTRLELGDVQYLHLLPQTNATCVLNFHMTPAVSLAQKVSAIAAILKRSENKHGQYTFDIPTVANDMGVTPVELTNQLYALKLKGEITYEMKDMAYCYRILEAPADFFSLSADITRWLSEVECCKVRKMDAMFNAAYFAVNLCDKMSGCSSSDHTSCLQRIILDYFAGVDNADFCKKIDQSSRFLRADIKVFLQSNSHARFTPRAVARVMHGIASPAYPYTIWGKTHFWGRYTKIDFNVVMEAAKEELKTFIGKDYSSNL

Target ORF information:

RefSeq Version	XM_013591073.2
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula ATP-dependent DNA helicase Q-like 5 (LOC25501716), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013591073.2

ORF Insert Sequence:

ATGCAGTTTG ATTCCGATTC AGATTCCGAC GGTTCTCACA TTTCCTCAAC TCCACCAAGA 
CAACAACCAA ATTCACCACC ACCACCAAAA CCACCTCTTC CAGTTTCAAA CAAAAAATCC 
AAAACCAAAA CCAAAACCTC AAAATCCAAT AAACCCCTTT CTAAACCCCC CGAAATTCTT 
CCCGAACAAG AATCTTTCCC CTTCCCTTCC ATCCCCTCAC TTCCTTTCAA CATCCGACAA 
CGAACGTCTG ACGCTCCTTC AACTTCATCT TCTCACTCTA TCGAAACCCT CCCTGCCGGA 
TTCTTCTCCA AATCAATTTC ATTCTCCAAA ATTCGTAAAC CCTTACTCAA CCTAGAAACA 
TCCGAAGCCG AACCGGTTGT TGATTCTGCA AAAACTGTAG CGAAATCTGT GAAGAAACAT 
CCGAATTTGA TAGGTGCTAG TGTGGTAACG CCGATAGTGA AGGCACTAAA AGGCGCTGGA 
GAAGGAAATT TTGTGAAATT GAATCTCAAT GGTAAGAGGA AAAAGTTTTT GAATAAAGGA 
TGGAAGAAAA ATGGAAAATT TGGTAGTGGT AAAAGGTATT TTAGAAATAG TAATGGTAGT 
AATAGTGGTA GAAGTAGTAG AAGTAAACGT ATAAAATCGG AATACAAAGA AGACGATGAT 
GATGATGAGG GGGATGGTAT GGGTGTTGAG AATGCAAAGC AACAGGAAAA GAAGAATGGT 
TGGGAAAATG AATGCGAAGT GTTGGAGGAG GCTGTGTTAG CAGCAAGAGA TGATGCTTCT 
GATGAGAATT TGGTGAAGTT GTTGAAATTG ATTCATGGTT TTGATTCTTT TCGAGAGGGG 
CAGCTTGAGG CGATTAAGAA TGTGCTTGCT GGGAAATCGA CGATGCTTAT TTTGCCGACT 
GGTGCTGGGA AATCGTTGTG TTATCAATTG AGTGCGTTGA TTTTGCCTGG GATTACGCTT 
GTTGTTAGTC CTTTGGTAGC TTTGATGATT GATCAGCTTC GACAATTGCC TCCGCTCATT 
TCGGGCGCTC TTCTAAGCAG TGCTCAGACA TTTGAAGAAT CCTCTGAAAC GCTTAACCAG 
CTTCGTCAAG GCACAATAAA GGTGCTTTTT GTTTCACCAG AGCGATTCTT GAATGAAGAA 
TTTCTGTCTG TTATCTCTGG TGGGTTAGCC GTCTCACTTG TTGTTATTGA TGAAGCACAT 
TGTATATCTG AATGGTCTCA CAATTTCCGA CCTTCATTCA TGAGACTTAG AGCATCACTG 
CTTCATAAGA GTCTTAATGT AGGTTCTATC CTTGCAATGA CAGCAACTGC CACAACCACG 
ACTTTAGATG CCATTATGTC TGCCCTGGAT ATTCCTCGCA CAAATCTTAT TCAGAATGCA 
CACTTAAGGG ACAATTTGTG TTTGTCTGTG TCTTTGATAA AAAATAGAAT GAAAGACCTA 
CTGGTGCTTA TGAAGTCTTC TCCTTTTGCG GAAGTTAAAA GCATCATAGT ATACTGCAAA 
TTTCAGTCTG AAACTGATCA AATTAGCAGA TATTTGAATG ATAACAATAT CAAGGCAAAG 
AGTTATCATA GTGGTATTTC TGCAAAGGAA CGTGGCTACG TACAGGAGTT GTTTGGTTCA 
AACAAGATCA GAGTGGTTGT TGCAACTGTG GCATTTGGCA TGGGACTAGA TAAAAGAGAT 
GTTGGAGCAG TAATTCATTA CAGTTTACCT GGAAGTCTGG AAGAGTATGT ACAGGAGATA 
GGACGTGCCG GAAGGGATGG GAGGTTGTCC TATTGTCACC TATTTTATGA TGATGAGATG 
TATTTCAAAC TTCGTAGTCT AATGTACAGT GAAGGAATAG ATGAATATGC AGTGAATAAA 
TTTCTGTCTG AAGTGTTTCC TGCTGACAAA AGCTCCTGTG GGAAAATATG TTCGTTAATC 
AAAGAACCTG CTTCACGCAG GTTTGATATG AAAGAAGAGG TGATTCTCAC AGTTTTGACA 
CGCTTGGAGC TGGGTGACGT GCAGTACTTA CATTTACTTC CACAGACAAA TGCAACGTGT 
GTTCTGAATT TTCATATGAC TCCGGCTGTG TCCCTTGCCC AAAAGGTTTC TGCTATTGCT 
GCAATTCTGA AAAGATCTGA AAATAAGCAC GGACAATATA CTTTTGATAT TCCAACTGTG 
GCAAATGACA TGGGGGTCAC ACCAGTTGAA TTAACAAATC AGTTGTATGC CCTAAAGTTG 
AAGGGAGAAA TAACTTATGA AATGAAGGAC ATGGCCTACT GTTATAGGAT TCTTGAAGCC 
CCAGCAGACT TTTTTTCTCT ATCAGCAGAT ATTACACGAT GGTTATCGGA AGTTGAATGT 
TGTAAGGTAC GAAAAATGGA TGCAATGTTT AATGCTGCAT ATTTTGCAGT AAATCTATGT 
GATAAGATGA GTGGTTGCAG TAGTTCTGAT CACACTTCAT GCTTGCAAAG GATAATATTG 
GATTACTTTG CTGGAGTTGA TAATGCTGAT TTCTGCAAGA AAATTGATCA AAGCAGCCGT 
TTCCTGCGGG CAGATATAAA GGTTTTTCTA CAGAGCAATT CACATGCTCG CTTTACACCA 
AGAGCTGTTG CGAGGGTAAT GCATGGAATT GCAAGCCCAG CCTACCCTTA TACAATATGG 
GGTAAAACCC ATTTCTGGGG AAGGTATACC AAGATAGACT TTAACGTTGT GATGGAAGCA 
GCTAAGGAAG AACTTAAAAC ATTTATTGGA AAAGATTACT CTAGCAATTT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
BMC genomics15312(2014 Apr)
Tang H,Krishnakumar V,Bidwell S,Rosen B,Chan A,Zhou S,Gentzbittel L,Childs KL,Yandell M,Gundlach H,Mayer KF,Schwartz DC,Town CD

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Nature480(7378)520-4(2011 Nov)
Young ND,Debell? F,Oldroyd GE,Geurts R,Cannon SB,Udvardi MK,Benedito VA,Mayer KF,Gouzy J,Schoof H,Van de Peer Y,Proost S,Cook DR,Meyers BC,Spannagl M,Cheung F,De Mita S,Krishnakumar V,Gundlach H,Zhou S,Mudge J,Bharti AK,Murray JD,Naoumkina MA,Rosen B,Silverstein KA,Tang H,Rombauts S,Zhao PX,Zhou P,Barbe V,Bardou P,Bechner M,Bellec A,Berger A,Berg?s H,Bidwell S,Bisseling T,Choisne N,Couloux A,Denny R,Deshpande S,Dai X,Doyle JJ,Dudez AM,Farmer AD,Fouteau S,Franken C,Gibelin C,Gish J,Goldstein S,Gonz?lez AJ,Green PJ,Hallab A,Hartog M,Hua A,Humphray SJ,Jeong DH,Jing Y,J?cker A,Kenton SM,Kim DJ,Klee K,Lai H,Lang C,Lin S,Macmil SL,Magdelenat G,Matthews L,McCorrison J,Monaghan EL,Mun JH,Najar FZ,Nicholson C,Noirot C,O'Bleness M,Paule CR,Poulain J,Prion F,Qin B,Qu C,Retzel EF,Riddle C,Sallet E,Samain S,Samson N,Sanders I,Saurat O,Scarpelli C,Schiex T,Segurens B,Severin AJ,Sherrier DJ,Shi R,Sims S,Singer SR,Sinharoy S,Sterck L,Viollet A,Wang BB,Wang K,Wang M,Wang X,Warfsmann J,Weissenbach J,White DD,White JD,Wiley GB,Wincker P,Xing Y,Yang L,Yao Z,Ying F,Zhai J,Zhou L,Zuber A,D?nari? J,Dixon RA,May GD,Schwartz DC,Rogers J,Qu?tier F,Town CD,Roe BA

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LOC25501716 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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