usp28 cDNA ORF clone, Maylandia zebra(zebra mbuna)

Gene
Clones
gRNAs

Summary

Gene Symbol	usp28
Entrez Gene ID	101469367
Full Name	ubiquitin specific peptidase 28
Gene Type	protein-coding
Organism	Maylandia zebra(zebra mbuna)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023153119.1	XP_023008887.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X3
XM_004573473.3	XP_004573530.1	ubiquitin carboxyl-terminal hydrolase 28
XM_023153118.1	XP_023008886.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X2
XM_004573473.5	XP_004573530.2	ubiquitin carboxyl-terminal hydrolase 28 isoform X1
XM_004573473.4	XP_004573530.1	ubiquitin carboxyl-terminal hydrolase 28 isoform X1
XM_023153119.2	XP_023008887.2	ubiquitin carboxyl-terminal hydrolase 28 isoform X3
XM_023153118.2	XP_023008886.2	ubiquitin carboxyl-terminal hydrolase 28 isoform X2

Pathways from BioSystems
Gene Ontology

The following usp28 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the usp28 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMa06379	XM_004573473.4 Latest version!	Maylandia zebra ubiquitin specific peptidase 28 (usp28), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OMa313080	XM_023153118.1 Latest version!	Maylandia zebra ubiquitin specific peptidase 28 (usp28), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OMa313081	XM_023153119.1 Latest version!	Maylandia zebra ubiquitin specific peptidase 28 (usp28), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OMa359731	XM_004573473.5 Latest version!	Maylandia zebra ubiquitin specific peptidase 28 (usp28), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMa359732	XM_023153118.2 Latest version!	Maylandia zebra ubiquitin specific peptidase 28 (usp28), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMa359733	XM_023153119.2 Latest version!	Maylandia zebra ubiquitin specific peptidase 28 (usp28), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OMa06379	XM_004573473.3	Maylandia zebra ubiquitin specific peptidase 28 (usp28), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

usp28 ( XM_004573473.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004573530.1 Maylandia zebra ubiquitin specific peptidase 28 (usp28), mRNA.

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CloneID	OMa06379
Clone ID Related Accession (Same CDS sequence)	XM_004573473.3 , XM_004573473.4
Accession Version	XM_004573473.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-10-15
Organism	Maylandia zebra(Zebra mbuna)
Product	ubiquitin carboxyl-terminal hydrolase 28
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014444930.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 16, 2015 this sequence version replaced XM_004573473.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Maylandia zebra Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTCGT CTGCAATGAG AGCCGATCAG TCTGAAAATG GAGAAAACTC AACCAACTCG 
CAGAGTGAAA TGCTGATCAG CCAGCTGAGG GAGATCACTG GCATCCAGGA CCAACAGATC 
CTCTACAGAG CCCTGAATGT CAGTCAGGGG GACATTGGAC ATGCTGTTGG ACTGCTGACA 
ACCCAGACTG CAGAGGTTCA GGACCCTGGA GAACCACAGG AGTCAGGGAC ATCTGGAGAG 
GCCTGGGAGG GCCAGAAAGG GCTTCCTAAA GATGAACTGC AGACCGCTAT AGAACTCAGC 
CTGCAGGAGT CCCACAAAGC CCAGGAAGAG GAGCGAGAAT TTCATAGGGC TCTGGAAGCC 
AGTGCAGAGG AGAATGCTGC GAGAATAAAG AGGAAGAGAT GTGAAGCCCA GAGTGAAATG 
TGTAGCCCTG CAGAGTGGAT CCGTCAGGAA GATTGGCCTG TGGGCATTCG TAATGTAGGA 
AACACCTGCT GGTTCAGTGC TGTCATCCAG TCACTCTTCC ACCTGCCTGT GTTCAGGAGA 
CTGGTTCTCA ACTACCATCT GTCTGAGAAA ATACTGGAGA AGTGTAAAAG CCATTCAGAC 
AAGAGGAACA TAGCCTTCAT GCAGGAGCTG CGCTGCCTGT TTGCCCTCAT GGTGGGCTCT 
ACCCGCAGGT TTGTGGATCC TTCTGCTGCT GTGGAGTTAT TGCGTGACGC CTTCAGGACC 
AATGAGGCCC AACAGGATGT CAGTGAGTTT TCCCACAAAC TGCTTGACTG GCTCGAGGAT 
GCATTTCAGC TGGCTGCCAG TGGAAAAAAT GCAGAAGATA AACAACATAA CCCCATGGTC 
CAGCTCTTCT ATGGTACCTT TGTGACAGAA AGAAGACACG AGGGTAAGAT GGTATATAAT 
ATTGAGCAGT TTGGCCAGTA CCCCCTGCAA GTCAATGGCT TCAACAATCT TGATGAATGC 
TTAGAAGGTG CTATGGTAGA GAAGGAGATT GAGTCGGTTC ATTCAGAGCA TCATGTCACA 
TCTGGACGTG AGAGATGGTT CAAAAAGCTA CCACCAGTCT TGACATTTGA ACTTTCGAGA 
TTTGAATTCA ACACCCAGCT TGGGCGCCCT GAGAAGATAC ATAAGAAACT GGAGTTTCCT 
CAAATCATTT ATATGGACAG ATATCTTCAC AAAAACATTG AGCAGACCCA TGAGAGGAGA 
GGAGAAGTAA AGAAACTAAA GGAGCAACTG GCAGTCCTTC AACAGAAACT TGAGTGTTAT 
AAAAACTATG GCTCTGGACC TACAAAGTAC CCTCTAGCTG ACATGCTCCA GTTTGTGCTG 
GAGTTTGCTA CCACCAAGCC GACTAGCGTT TCCCCAGCTG AAGACTCGAG ACAGGCCGCA 
TCGTCACCGA CTCCTCCAAG TGCCCCCCTC AGTGAAGCCA TTGCCAAAGA AAGCAGTGAT 
CCCGGAGAAT CAGTTTCATC AGAGAGCCTG GGTTCCAGTG TTTCCAGTTG CCAGAGGACC 
CCCATATACA AGCCCTTCAC TCAGTGCAGA CAACCTATTG ACTGCCCACC ACACCCTGCC 
CCCCACAGCA TCACAGAAGA AGAGCTGCAC TTTGCTAAAA CCTGTCTTCA GCGTTGGAGG 
ACTGAAGTTG AGAATGACAT AAATGAACTA AAGGCCAGCA TTGACAGGCT CACTCAGACG 
CTCGAGGGCA TGTACTCAGA CAACAGTCTC TGCCAGGCCC CCTACAGACT CCATGCAGTG 
CTTGTCCATG AAGGCCAGGC ATCAGCTGGT CATTACTGGG CTTACATCTA TGACCATGCT 
AACCAGCGCT GGCTGAAGTA CAATGACATA AGTGTCACTG AGTCATCGTG GGATGAGCTG 
GAGCGAGACT CCTTTGGAGG CATGACCAAC GCAAGTGCCT ACTGCCTAAT GTACATTGAC 
GACAGGCTAC CTCAGCTCAT CACAGAAGAC ACAGATGATG AGACGGGCCA GGTGCTCCAT 
GGCATGGACT CTCTGCCACC CATCCTCAGG CGCTTTGTCC ACGAAGATAA CCGCTGGTTC 
CAGCAGGAGC TCAGCGAATG GGAGGAGCAG TTCTGCCAGA CAGCAACCCC GCAGGAAGAG 
TCCACCGCCT CTGCAGAGCC CACAAGTCCC AGTCTGGATA ACATACAGCA GTCACCTGTT 
GAGCCAGCAC CCCAGTCAGG ATCCACTACT GAAGAGGTGG ATGAAGGAGT TGCTCCAGAG 
CCACAGCCAG ACCAAGAAGC AGAGAAAGAG CCAGAACCTG ATCTCAGAAA AGAGCCAGAA 
GCGCAAACAG ATTTATCTCC CCCACCACCA GAGGGCCCTG GCTGCCAGCC AGGTGATACC 
AGCACTCCAA CAGTCACTGA CACCACGGGG GTCAGCCAGA GCTCTGATTC AGAGGAGAAG 
GAGCCGCAGA ATCAGATCCC TGGCCCTGAT GTAGAGCTTT GCGATCAGAC ACCATCTGAC 
ATCCACGTAG AGAAGGATAA CCCAGAGGTG GCCGGCCTCG TCTCTGAAAC TGAAGAAGAG 
CCCCAGGCTT CTGGAGAGGC TCCTGAACCT ATCGCAGAGC AAAGCAATCA GGAGCAGCAG 
CAGCAGCAGC AGGAGGTTCC AGCCAGACCA CGACAGACTG AGAATGAGGT CTCAGAAGTG 
GAGATCCCCA ATGTGGGCCG GATCATGGTG AGGGCGGATG CTGACGGATA CAATGAAGAG 
ATGATGCTAA CTCCAGCTAT GCAGGGTGTC ATTCTAGCCA TAGCCAAGGC CAGACAAATT 
TTTGATAAGG AGGGTCCTGA GGCTGGCCTC ATCAAGGCTT TCCATGAAGA GTATTCGCGC 
TTGTATGAGC TCTCTGAAGA GGAGACCACA CCACAGGAGG ATGCACGTCT CCAGCACGCT 
CTGGTCTACT TCTTCCAGAA CAAGGCACCC AAGCGTGTCA TTGAGAGGAC GCTGCTTGAG 
CAGTTTACTG ACCGCAACCT CAGTTTTGAT GAGAGAGCCA TCAGTATCAT GAGGGAAGCC 
CGGGCCAAAC TTCGTCTCAT TAAGCCAGAA GACATGGACA TGGAAGAATA CATGCAGTGG 
CATGACGATT ACAGGCTGTT CAGGACGGTC TTTGTCTATC TGTTGACTGG GCTGGAGCAT 
TACCAGCACG GGAAGATGCG TGATGCACTG AATTACTTGG CCCACGCCTA TGAGACCAAC 
AGCACCCTGC TGCGAAACGG AGAGAAACGT GGTGTGGACA AGTCTCTCAT TGCTGTTTAC 
AGGAGGAAGT GTCTCACTGC ACTAAATGAG AGCGCATCGC AGCTATTTTG CAGCGGTGAG 
GAGGCCAAAG TAGAAGAGGG TATTGCCATC ATGGATGAAG CCGTCATCCC CTGCCTTCAC 
CTGATGAACC GGGACTCGAC TTTACCCCAG GAAGACCGAG ATGCAATGGA GGCCATCCGC 
AGCCACTGGT GCTGCTGCCT GGGCCGAGAC ATGGATCAGT CATTGCAGGT GAAGCTCGGC 
GAGTTGCTTC CCAGAGTTCT GGACGGTTCA GCTGACACAG TAGTGCTTAA AGACCCACCC 
AAAGTCCACG TCACCCAGGC CTACGACCTG TGCAGTCGCC TGGCTGCTGT TATGGAATCC 
ATCCACAACA CATCAGTGGT CATTGTGAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004573530.1
CDS	107..3739
Translation	MFSSAMRADQSENGENSTNSQSEMLISQLREITGIQDQQILYRALNVSQGDIGHAVGLLTTQTAEVQDPGEPQESGTSGEAWEGQKGLPKDELQTAIELSLQESHKAQEEEREFHRALEASAEENAARIKRKRCEAQSEMCSPAEWIRQEDWPVGIRNVGNTCWFSAVIQSLFHLPVFRRLVLNYHLSEKILEKCKSHSDKRNIAFMQELRCLFALMVGSTRRFVDPSAAVELLRDAFRTNEAQQDVSEFSHKLLDWLEDAFQLAASGKNAEDKQHNPMVQLFYGTFVTERRHEGKMVYNIEQFGQYPLQVNGFNNLDECLEGAMVEKEIESVHSEHHVTSGRERWFKKLPPVLTFELSRFEFNTQLGRPEKIHKKLEFPQIIYMDRYLHKNIEQTHERRGEVKKLKEQLAVLQQKLECYKNYGSGPTKYPLADMLQFVLEFATTKPTSVSPAEDSRQAASSPTPPSAPLSEAIAKESSDPGESVSSESLGSSVSSCQRTPIYKPFTQCRQPIDCPPHPAPHSITEEELHFAKTCLQRWRTEVENDINELKASIDRLTQTLEGMYSDNSLCQAPYRLHAVLVHEGQASAGHYWAYIYDHANQRWLKYNDISVTESSWDELERDSFGGMTNASAYCLMYIDDRLPQLITEDTDDETGQVLHGMDSLPPILRRFVHEDNRWFQQELSEWEEQFCQTATPQEESTASAEPTSPSLDNIQQSPVEPAPQSGSTTEEVDEGVAPEPQPDQEAEKEPEPDLRKEPEAQTDLSPPPPEGPGCQPGDTSTPTVTDTTGVSQSSDSEEKEPQNQIPGPDVELCDQTPSDIHVEKDNPEVAGLVSETEEEPQASGEAPEPIAEQSNQEQQQQQQEVPARPRQTENEVSEVEIPNVGRIMVRADADGYNEEMMLTPAMQGVILAIAKARQIFDKEGPEAGLIKAFHEEYSRLYELSEEETTPQEDARLQHALVYFFQNKAPKRVIERTLLEQFTDRNLSFDERAISIMREARAKLRLIKPEDMDMEEYMQWHDDYRLFRTVFVYLLTGLEHYQHGKMRDALNYLAHAYETNSTLLRNGEKRGVDKSLIAVYRRKCLTALNESASQLFCSGEEAKVEEGIAIMDEAVIPCLHLMNRDSTLPQEDRDAMEAIRSHWCCCLGRDMDQSLQVKLGELLPRVLDGSADTVVLKDPPKVHVTQAYDLCSRLAAVMESIHNTSVVIVK

Target ORF information:

RefSeq Version	XM_004573473.3
Organism	Maylandia zebra(Zebra mbuna)
Definition	Maylandia zebra ubiquitin specific peptidase 28 (usp28), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004573473.3

ORF Insert Sequence:

ATGTTTTCGT CTGCAATGAG AGCCGATCAG TCTGAAAATG GAGAAAACTC AACCAACTCG 
CAGAGTGAAA TGCTGATCAG CCAGCTGAGG GAGATCACTG GCATCCAGGA CCAACAGATC 
CTCTACAGAG CCCTGAATGT CAGTCAGGGG GACATTGGAC ATGCTGTTGG ACTGCTGACA 
ACCCAGACTG CAGAGGTTCA GGACCCTGGA GAACCACAGG AGTCAGGGAC ATCTGGAGAG 
GCCTGGGAGG GCCAGAAAGG GCTTCCTAAA GATGAACTGC AGACCGCTAT AGAACTCAGC 
CTGCAGGAGT CCCACAAAGC CCAGGAAGAG GAGCGAGAAT TTCATAGGGC TCTGGAAGCC 
AGTGCAGAGG AGAATGCTGC GAGAATAAAG AGGAAGAGAT GTGAAGCCCA GAGTGAAATG 
TGTAGCCCTG CAGAGTGGAT CCGTCAGGAA GATTGGCCTG TGGGCATTCG TAATGTAGGA 
AACACCTGCT GGTTCAGTGC TGTCATCCAG TCACTCTTCC ACCTGCCTGT GTTCAGGAGA 
CTGGTTCTCA ACTACCATCT GTCTGAGAAA ATACTGGAGA AGTGTAAAAG CCATTCAGAC 
AAGAGGAACA TAGCCTTCAT GCAGGAGCTG CGCTGCCTGT TTGCCCTCAT GGTGGGCTCT 
ACCCGCAGGT TTGTGGATCC TTCTGCTGCT GTGGAGTTAT TGCGTGACGC CTTCAGGACC 
AATGAGGCCC AACAGGATGT CAGTGAGTTT TCCCACAAAC TGCTTGACTG GCTCGAGGAT 
GCATTTCAGC TGGCTGCCAG TGGAAAAAAT GCAGAAGATA AACAACATAA CCCCATGGTC 
CAGCTCTTCT ATGGTACCTT TGTGACAGAA AGAAGACACG AGGGTAAGAT GGTATATAAT 
ATTGAGCAGT TTGGCCAGTA CCCCCTGCAA GTCAATGGCT TCAACAATCT TGATGAATGC 
TTAGAAGGTG CTATGGTAGA GAAGGAGATT GAGTCGGTTC ATTCAGAGCA TCATGTCACA 
TCTGGACGTG AGAGATGGTT CAAAAAGCTA CCACCAGTCT TGACATTTGA ACTTTCGAGA 
TTTGAATTCA ACACCCAGCT TGGGCGCCCT GAGAAGATAC ATAAGAAACT GGAGTTTCCT 
CAAATCATTT ATATGGACAG ATATCTTCAC AAAAACATTG AGCAGACCCA TGAGAGGAGA 
GGAGAAGTAA AGAAACTAAA GGAGCAACTG GCAGTCCTTC AACAGAAACT TGAGTGTTAT 
AAAAACTATG GCTCTGGACC TACAAAGTAC CCTCTAGCTG ACATGCTCCA GTTTGTGCTG 
GAGTTTGCTA CCACCAAGCC GACTAGCGTT TCCCCAGCTG AAGACTCGAG ACAGGCCGCA 
TCGTCACCGA CTCCTCCAAG TGCCCCCCTC AGTGAAGCCA TTGCCAAAGA AAGCAGTGAT 
CCCGGAGAAT CAGTTTCATC AGAGAGCCTG GGTTCCAGTG TTTCCAGTTG CCAGAGGACC 
CCCATATACA AGCCCTTCAC TCAGTGCAGA CAACCTATTG ACTGCCCACC ACACCCTGCC 
CCCCACAGCA TCACAGAAGA AGAGCTGCAC TTTGCTAAAA CCTGTCTTCA GCGTTGGAGG 
ACTGAAGTTG AGAATGACAT AAATGAACTA AAGGCCAGCA TTGACAGGCT CACTCAGACG 
CTCGAGGGCA TGTACTCAGA CAACAGTCTC TGCCAGGCCC CCTACAGACT CCATGCAGTG 
CTTGTCCATG AAGGCCAGGC ATCAGCTGGT CATTACTGGG CTTACATCTA TGACCATGCT 
AACCAGCGCT GGCTGAAGTA CAATGACATA AGTGTCACTG AGTCATCGTG GGATGAGCTG 
GAGCGAGACT CCTTTGGAGG CATGACCAAC GCAAGTGCCT ACTGCCTAAT GTACATTGAC 
GACAGGCTAC CTCAGCTCAT CACAGAAGAC ACAGATGATG AGACGGGCCA GGTGCTCCAT 
GGCATGGACT CTCTGCCACC CATCCTCAGG CGCTTTGTCC ACGAAGATAA CCGCTGGTTC 
CAGCAGGAGC TCAGCGAATG GGAGGAGCAG TTCTGCCAGA CAGCAACCCC GCAGGAAGAG 
TCCACCGCCT CTGCAGAGCC CACAAGTCCC AGTCTGGATA ACATACAGCA GTCACCTGTT 
GAGCCAGCAC CCCAGTCAGG ATCCACTACT GAAGAGGTGG ATGAAGGAGT TGCTCCAGAG 
CCACAGCCAG ACCAAGAAGC AGAGAAAGAG CCAGAACCTG ATCTCAGAAA AGAGCCAGAA 
GCGCAAACAG ATTTATCTCC CCCACCACCA GAGGGCCCTG GCTGCCAGCC AGGTGATACC 
AGCACTCCAA CAGTCACTGA CACCACGGGG GTCAGCCAGA GCTCTGATTC AGAGGAGAAG 
GAGCCGCAGA ATCAGATCCC TGGCCCTGAT GTAGAGCTTT GCGATCAGAC ACCATCTGAC 
ATCCACGTAG AGAAGGATAA CCCAGAGGTG GCCGGCCTCG TCTCTGAAAC TGAAGAAGAG 
CCCCAGGCTT CTGGAGAGGC TCCTGAACCT ATCGCAGAGC AAAGCAATCA GGAGCAGCAG 
CAGCAGCAGC AGGAGGTTCC AGCCAGACCA CGACAGACTG AGAATGAGGT CTCAGAAGTG 
GAGATCCCCA ATGTGGGCCG GATCATGGTG AGGGCGGATG CTGACGGATA CAATGAAGAG 
ATGATGCTAA CTCCAGCTAT GCAGGGTGTC ATTCTAGCCA TAGCCAAGGC CAGACAAATT 
TTTGATAAGG AGGGTCCTGA GGCTGGCCTC ATCAAGGCTT TCCATGAAGA GTATTCGCGC 
TTGTATGAGC TCTCTGAAGA GGAGACCACA CCACAGGAGG ATGCACGTCT CCAGCACGCT 
CTGGTCTACT TCTTCCAGAA CAAGGCACCC AAGCGTGTCA TTGAGAGGAC GCTGCTTGAG 
CAGTTTACTG ACCGCAACCT CAGTTTTGAT GAGAGAGCCA TCAGTATCAT GAGGGAAGCC 
CGGGCCAAAC TTCGTCTCAT TAAGCCAGAA GACATGGACA TGGAAGAATA CATGCAGTGG 
CATGACGATT ACAGGCTGTT CAGGACGGTC TTTGTCTATC TGTTGACTGG GCTGGAGCAT 
TACCAGCACG GGAAGATGCG TGATGCACTG AATTACTTGG CCCACGCCTA TGAGACCAAC 
AGCACCCTGC TGCGAAACGG AGAGAAACGT GGTGTGGACA AGTCTCTCAT TGCTGTTTAC 
AGGAGGAAGT GTCTCACTGC ACTAAATGAG AGCGCATCGC AGCTATTTTG CAGCGGTGAG 
GAGGCCAAAG TAGAAGAGGG TATTGCCATC ATGGATGAAG CCGTCATCCC CTGCCTTCAC 
CTGATGAACC GGGACTCGAC TTTACCCCAG GAAGACCGAG ATGCAATGGA GGCCATCCGC 
AGCCACTGGT GCTGCTGCCT GGGCCGAGAC ATGGATCAGT CATTGCAGGT GAAGCTCGGC 
GAGTTGCTTC CCAGAGTTCT GGACGGTTCA GCTGACACAG TAGTGCTTAA AGACCCACCC 
AAAGTCCACG TCACCCAGGC CTACGACCTG TGCAGTCGCC TGGCTGCTGT TATGGAATCC 
ATCCACAACA CATCAGTGGT CATTGTGAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp28 ( XM_004573473.4 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004573530.1 Maylandia zebra ubiquitin specific peptidase 28 (usp28), transcript variant X1, mRNA.

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CloneID	OMa06379
Clone ID Related Accession (Same CDS sequence)	XM_004573473.3 , XM_004573473.4
Accession Version	XM_004573473.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-29
Organism	Maylandia zebra(zebra mbuna)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014444930.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 30, 2017 this sequence version replaced XM_004573473.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTCGT CTGCAATGAG AGCCGATCAG TCTGAAAATG GAGAAAACTC AACCAACTCG 
CAGAGTGAAA TGCTGATCAG CCAGCTGAGG GAGATCACTG GCATCCAGGA CCAACAGATC 
CTCTACAGAG CCCTGAATGT CAGTCAGGGG GACATTGGAC ATGCTGTTGG ACTGCTGACA 
ACCCAGACTG CAGAGGTTCA GGACCCTGGA GAACCACAGG AGTCAGGGAC ATCTGGAGAG 
GCCTGGGAGG GCCAGAAAGG GCTTCCTAAA GATGAACTGC AGACCGCTAT AGAACTCAGC 
CTGCAGGAGT CCCACAAAGC CCAGGAAGAG GAGCGAGAAT TTCATAGGGC TCTGGAAGCC 
AGTGCAGAGG AGAATGCTGC GAGAATAAAG AGGAAGAGAT GTGAAGCCCA GAGTGAAATG 
TGTAGCCCTG CAGAGTGGAT CCGTCAGGAA GATTGGCCTG TGGGCATTCG TAATGTAGGA 
AACACCTGCT GGTTCAGTGC TGTCATCCAG TCACTCTTCC ACCTGCCTGT GTTCAGGAGA 
CTGGTTCTCA ACTACCATCT GTCTGAGAAA ATACTGGAGA AGTGTAAAAG CCATTCAGAC 
AAGAGGAACA TAGCCTTCAT GCAGGAGCTG CGCTGCCTGT TTGCCCTCAT GGTGGGCTCT 
ACCCGCAGGT TTGTGGATCC TTCTGCTGCT GTGGAGTTAT TGCGTGACGC CTTCAGGACC 
AATGAGGCCC AACAGGATGT CAGTGAGTTT TCCCACAAAC TGCTTGACTG GCTCGAGGAT 
GCATTTCAGC TGGCTGCCAG TGGAAAAAAT GCAGAAGATA AACAACATAA CCCCATGGTC 
CAGCTCTTCT ATGGTACCTT TGTGACAGAA AGAAGACACG AGGGTAAGAT GGTATATAAT 
ATTGAGCAGT TTGGCCAGTA CCCCCTGCAA GTCAATGGCT TCAACAATCT TGATGAATGC 
TTAGAAGGTG CTATGGTAGA GAAGGAGATT GAGTCGGTTC ATTCAGAGCA TCATGTCACA 
TCTGGACGTG AGAGATGGTT CAAAAAGCTA CCACCAGTCT TGACATTTGA ACTTTCGAGA 
TTTGAATTCA ACACCCAGCT TGGGCGCCCT GAGAAGATAC ATAAGAAACT GGAGTTTCCT 
CAAATCATTT ATATGGACAG ATATCTTCAC AAAAACATTG AGCAGACCCA TGAGAGGAGA 
GGAGAAGTAA AGAAACTAAA GGAGCAACTG GCAGTCCTTC AACAGAAACT TGAGTGTTAT 
AAAAACTATG GCTCTGGACC TACAAAGTAC CCTCTAGCTG ACATGCTCCA GTTTGTGCTG 
GAGTTTGCTA CCACCAAGCC GACTAGCGTT TCCCCAGCTG AAGACTCGAG ACAGGCCGCA 
TCGTCACCGA CTCCTCCAAG TGCCCCCCTC AGTGAAGCCA TTGCCAAAGA AAGCAGTGAT 
CCCGGAGAAT CAGTTTCATC AGAGAGCCTG GGTTCCAGTG TTTCCAGTTG CCAGAGGACC 
CCCATATACA AGCCCTTCAC TCAGTGCAGA CAACCTATTG ACTGCCCACC ACACCCTGCC 
CCCCACAGCA TCACAGAAGA AGAGCTGCAC TTTGCTAAAA CCTGTCTTCA GCGTTGGAGG 
ACTGAAGTTG AGAATGACAT AAATGAACTA AAGGCCAGCA TTGACAGGCT CACTCAGACG 
CTCGAGGGCA TGTACTCAGA CAACAGTCTC TGCCAGGCCC CCTACAGACT CCATGCAGTG 
CTTGTCCATG AAGGCCAGGC ATCAGCTGGT CATTACTGGG CTTACATCTA TGACCATGCT 
AACCAGCGCT GGCTGAAGTA CAATGACATA AGTGTCACTG AGTCATCGTG GGATGAGCTG 
GAGCGAGACT CCTTTGGAGG CATGACCAAC GCAAGTGCCT ACTGCCTAAT GTACATTGAC 
GACAGGCTAC CTCAGCTCAT CACAGAAGAC ACAGATGATG AGACGGGCCA GGTGCTCCAT 
GGCATGGACT CTCTGCCACC CATCCTCAGG CGCTTTGTCC ACGAAGATAA CCGCTGGTTC 
CAGCAGGAGC TCAGCGAATG GGAGGAGCAG TTCTGCCAGA CAGCAACCCC GCAGGAAGAG 
TCCACCGCCT CTGCAGAGCC CACAAGTCCC AGTCTGGATA ACATACAGCA GTCACCTGTT 
GAGCCAGCAC CCCAGTCAGG ATCCACTACT GAAGAGGTGG ATGAAGGAGT TGCTCCAGAG 
CCACAGCCAG ACCAAGAAGC AGAGAAAGAG CCAGAACCTG ATCTCAGAAA AGAGCCAGAA 
GCGCAAACAG ATTTATCTCC CCCACCACCA GAGGGCCCTG GCTGCCAGCC AGGTGATACC 
AGCACTCCAA CAGTCACTGA CACCACGGGG GTCAGCCAGA GCTCTGATTC AGAGGAGAAG 
GAGCCGCAGA ATCAGATCCC TGGCCCTGAT GTAGAGCTTT GCGATCAGAC ACCATCTGAC 
ATCCACGTAG AGAAGGATAA CCCAGAGGTG GCCGGCCTCG TCTCTGAAAC TGAAGAAGAG 
CCCCAGGCTT CTGGAGAGGC TCCTGAACCT ATCGCAGAGC AAAGCAATCA GGAGCAGCAG 
CAGCAGCAGC AGGAGGTTCC AGCCAGACCA CGACAGACTG AGAATGAGGT CTCAGAAGTG 
GAGATCCCCA ATGTGGGCCG GATCATGGTG AGGGCGGATG CTGACGGATA CAATGAAGAG 
ATGATGCTAA CTCCAGCTAT GCAGGGTGTC ATTCTAGCCA TAGCCAAGGC CAGACAAATT 
TTTGATAAGG AGGGTCCTGA GGCTGGCCTC ATCAAGGCTT TCCATGAAGA GTATTCGCGC 
TTGTATGAGC TCTCTGAAGA GGAGACCACA CCACAGGAGG ATGCACGTCT CCAGCACGCT 
CTGGTCTACT TCTTCCAGAA CAAGGCACCC AAGCGTGTCA TTGAGAGGAC GCTGCTTGAG 
CAGTTTACTG ACCGCAACCT CAGTTTTGAT GAGAGAGCCA TCAGTATCAT GAGGGAAGCC 
CGGGCCAAAC TTCGTCTCAT TAAGCCAGAA GACATGGACA TGGAAGAATA CATGCAGTGG 
CATGACGATT ACAGGCTGTT CAGGACGGTC TTTGTCTATC TGTTGACTGG GCTGGAGCAT 
TACCAGCACG GGAAGATGCG TGATGCACTG AATTACTTGG CCCACGCCTA TGAGACCAAC 
AGCACCCTGC TGCGAAACGG AGAGAAACGT GGTGTGGACA AGTCTCTCAT TGCTGTTTAC 
AGGAGGAAGT GTCTCACTGC ACTAAATGAG AGCGCATCGC AGCTATTTTG CAGCGGTGAG 
GAGGCCAAAG TAGAAGAGGG TATTGCCATC ATGGATGAAG CCGTCATCCC CTGCCTTCAC 
CTGATGAACC GGGACTCGAC TTTACCCCAG GAAGACCGAG ATGCAATGGA GGCCATCCGC 
AGCCACTGGT GCTGCTGCCT GGGCCGAGAC ATGGATCAGT CATTGCAGGT GAAGCTCGGC 
GAGTTGCTTC CCAGAGTTCT GGACGGTTCA GCTGACACAG TAGTGCTTAA AGACCCACCC 
AAAGTCCACG TCACCCAGGC CTACGACCTG TGCAGTCGCC TGGCTGCTGT TATGGAATCC 
ATCCACAACA CATCAGTGGT CATTGTGAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004573530.1
CDS	89..3721
Translation	MFSSAMRADQSENGENSTNSQSEMLISQLREITGIQDQQILYRALNVSQGDIGHAVGLLTTQTAEVQDPGEPQESGTSGEAWEGQKGLPKDELQTAIELSLQESHKAQEEEREFHRALEASAEENAARIKRKRCEAQSEMCSPAEWIRQEDWPVGIRNVGNTCWFSAVIQSLFHLPVFRRLVLNYHLSEKILEKCKSHSDKRNIAFMQELRCLFALMVGSTRRFVDPSAAVELLRDAFRTNEAQQDVSEFSHKLLDWLEDAFQLAASGKNAEDKQHNPMVQLFYGTFVTERRHEGKMVYNIEQFGQYPLQVNGFNNLDECLEGAMVEKEIESVHSEHHVTSGRERWFKKLPPVLTFELSRFEFNTQLGRPEKIHKKLEFPQIIYMDRYLHKNIEQTHERRGEVKKLKEQLAVLQQKLECYKNYGSGPTKYPLADMLQFVLEFATTKPTSVSPAEDSRQAASSPTPPSAPLSEAIAKESSDPGESVSSESLGSSVSSCQRTPIYKPFTQCRQPIDCPPHPAPHSITEEELHFAKTCLQRWRTEVENDINELKASIDRLTQTLEGMYSDNSLCQAPYRLHAVLVHEGQASAGHYWAYIYDHANQRWLKYNDISVTESSWDELERDSFGGMTNASAYCLMYIDDRLPQLITEDTDDETGQVLHGMDSLPPILRRFVHEDNRWFQQELSEWEEQFCQTATPQEESTASAEPTSPSLDNIQQSPVEPAPQSGSTTEEVDEGVAPEPQPDQEAEKEPEPDLRKEPEAQTDLSPPPPEGPGCQPGDTSTPTVTDTTGVSQSSDSEEKEPQNQIPGPDVELCDQTPSDIHVEKDNPEVAGLVSETEEEPQASGEAPEPIAEQSNQEQQQQQQEVPARPRQTENEVSEVEIPNVGRIMVRADADGYNEEMMLTPAMQGVILAIAKARQIFDKEGPEAGLIKAFHEEYSRLYELSEEETTPQEDARLQHALVYFFQNKAPKRVIERTLLEQFTDRNLSFDERAISIMREARAKLRLIKPEDMDMEEYMQWHDDYRLFRTVFVYLLTGLEHYQHGKMRDALNYLAHAYETNSTLLRNGEKRGVDKSLIAVYRRKCLTALNESASQLFCSGEEAKVEEGIAIMDEAVIPCLHLMNRDSTLPQEDRDAMEAIRSHWCCCLGRDMDQSLQVKLGELLPRVLDGSADTVVLKDPPKVHVTQAYDLCSRLAAVMESIHNTSVVIVK

Target ORF information:

RefSeq Version	XM_004573473.4
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra ubiquitin specific peptidase 28 (usp28), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004573473.4

ORF Insert Sequence:

ATGTTTTCGT CTGCAATGAG AGCCGATCAG TCTGAAAATG GAGAAAACTC AACCAACTCG 
CAGAGTGAAA TGCTGATCAG CCAGCTGAGG GAGATCACTG GCATCCAGGA CCAACAGATC 
CTCTACAGAG CCCTGAATGT CAGTCAGGGG GACATTGGAC ATGCTGTTGG ACTGCTGACA 
ACCCAGACTG CAGAGGTTCA GGACCCTGGA GAACCACAGG AGTCAGGGAC ATCTGGAGAG 
GCCTGGGAGG GCCAGAAAGG GCTTCCTAAA GATGAACTGC AGACCGCTAT AGAACTCAGC 
CTGCAGGAGT CCCACAAAGC CCAGGAAGAG GAGCGAGAAT TTCATAGGGC TCTGGAAGCC 
AGTGCAGAGG AGAATGCTGC GAGAATAAAG AGGAAGAGAT GTGAAGCCCA GAGTGAAATG 
TGTAGCCCTG CAGAGTGGAT CCGTCAGGAA GATTGGCCTG TGGGCATTCG TAATGTAGGA 
AACACCTGCT GGTTCAGTGC TGTCATCCAG TCACTCTTCC ACCTGCCTGT GTTCAGGAGA 
CTGGTTCTCA ACTACCATCT GTCTGAGAAA ATACTGGAGA AGTGTAAAAG CCATTCAGAC 
AAGAGGAACA TAGCCTTCAT GCAGGAGCTG CGCTGCCTGT TTGCCCTCAT GGTGGGCTCT 
ACCCGCAGGT TTGTGGATCC TTCTGCTGCT GTGGAGTTAT TGCGTGACGC CTTCAGGACC 
AATGAGGCCC AACAGGATGT CAGTGAGTTT TCCCACAAAC TGCTTGACTG GCTCGAGGAT 
GCATTTCAGC TGGCTGCCAG TGGAAAAAAT GCAGAAGATA AACAACATAA CCCCATGGTC 
CAGCTCTTCT ATGGTACCTT TGTGACAGAA AGAAGACACG AGGGTAAGAT GGTATATAAT 
ATTGAGCAGT TTGGCCAGTA CCCCCTGCAA GTCAATGGCT TCAACAATCT TGATGAATGC 
TTAGAAGGTG CTATGGTAGA GAAGGAGATT GAGTCGGTTC ATTCAGAGCA TCATGTCACA 
TCTGGACGTG AGAGATGGTT CAAAAAGCTA CCACCAGTCT TGACATTTGA ACTTTCGAGA 
TTTGAATTCA ACACCCAGCT TGGGCGCCCT GAGAAGATAC ATAAGAAACT GGAGTTTCCT 
CAAATCATTT ATATGGACAG ATATCTTCAC AAAAACATTG AGCAGACCCA TGAGAGGAGA 
GGAGAAGTAA AGAAACTAAA GGAGCAACTG GCAGTCCTTC AACAGAAACT TGAGTGTTAT 
AAAAACTATG GCTCTGGACC TACAAAGTAC CCTCTAGCTG ACATGCTCCA GTTTGTGCTG 
GAGTTTGCTA CCACCAAGCC GACTAGCGTT TCCCCAGCTG AAGACTCGAG ACAGGCCGCA 
TCGTCACCGA CTCCTCCAAG TGCCCCCCTC AGTGAAGCCA TTGCCAAAGA AAGCAGTGAT 
CCCGGAGAAT CAGTTTCATC AGAGAGCCTG GGTTCCAGTG TTTCCAGTTG CCAGAGGACC 
CCCATATACA AGCCCTTCAC TCAGTGCAGA CAACCTATTG ACTGCCCACC ACACCCTGCC 
CCCCACAGCA TCACAGAAGA AGAGCTGCAC TTTGCTAAAA CCTGTCTTCA GCGTTGGAGG 
ACTGAAGTTG AGAATGACAT AAATGAACTA AAGGCCAGCA TTGACAGGCT CACTCAGACG 
CTCGAGGGCA TGTACTCAGA CAACAGTCTC TGCCAGGCCC CCTACAGACT CCATGCAGTG 
CTTGTCCATG AAGGCCAGGC ATCAGCTGGT CATTACTGGG CTTACATCTA TGACCATGCT 
AACCAGCGCT GGCTGAAGTA CAATGACATA AGTGTCACTG AGTCATCGTG GGATGAGCTG 
GAGCGAGACT CCTTTGGAGG CATGACCAAC GCAAGTGCCT ACTGCCTAAT GTACATTGAC 
GACAGGCTAC CTCAGCTCAT CACAGAAGAC ACAGATGATG AGACGGGCCA GGTGCTCCAT 
GGCATGGACT CTCTGCCACC CATCCTCAGG CGCTTTGTCC ACGAAGATAA CCGCTGGTTC 
CAGCAGGAGC TCAGCGAATG GGAGGAGCAG TTCTGCCAGA CAGCAACCCC GCAGGAAGAG 
TCCACCGCCT CTGCAGAGCC CACAAGTCCC AGTCTGGATA ACATACAGCA GTCACCTGTT 
GAGCCAGCAC CCCAGTCAGG ATCCACTACT GAAGAGGTGG ATGAAGGAGT TGCTCCAGAG 
CCACAGCCAG ACCAAGAAGC AGAGAAAGAG CCAGAACCTG ATCTCAGAAA AGAGCCAGAA 
GCGCAAACAG ATTTATCTCC CCCACCACCA GAGGGCCCTG GCTGCCAGCC AGGTGATACC 
AGCACTCCAA CAGTCACTGA CACCACGGGG GTCAGCCAGA GCTCTGATTC AGAGGAGAAG 
GAGCCGCAGA ATCAGATCCC TGGCCCTGAT GTAGAGCTTT GCGATCAGAC ACCATCTGAC 
ATCCACGTAG AGAAGGATAA CCCAGAGGTG GCCGGCCTCG TCTCTGAAAC TGAAGAAGAG 
CCCCAGGCTT CTGGAGAGGC TCCTGAACCT ATCGCAGAGC AAAGCAATCA GGAGCAGCAG 
CAGCAGCAGC AGGAGGTTCC AGCCAGACCA CGACAGACTG AGAATGAGGT CTCAGAAGTG 
GAGATCCCCA ATGTGGGCCG GATCATGGTG AGGGCGGATG CTGACGGATA CAATGAAGAG 
ATGATGCTAA CTCCAGCTAT GCAGGGTGTC ATTCTAGCCA TAGCCAAGGC CAGACAAATT 
TTTGATAAGG AGGGTCCTGA GGCTGGCCTC ATCAAGGCTT TCCATGAAGA GTATTCGCGC 
TTGTATGAGC TCTCTGAAGA GGAGACCACA CCACAGGAGG ATGCACGTCT CCAGCACGCT 
CTGGTCTACT TCTTCCAGAA CAAGGCACCC AAGCGTGTCA TTGAGAGGAC GCTGCTTGAG 
CAGTTTACTG ACCGCAACCT CAGTTTTGAT GAGAGAGCCA TCAGTATCAT GAGGGAAGCC 
CGGGCCAAAC TTCGTCTCAT TAAGCCAGAA GACATGGACA TGGAAGAATA CATGCAGTGG 
CATGACGATT ACAGGCTGTT CAGGACGGTC TTTGTCTATC TGTTGACTGG GCTGGAGCAT 
TACCAGCACG GGAAGATGCG TGATGCACTG AATTACTTGG CCCACGCCTA TGAGACCAAC 
AGCACCCTGC TGCGAAACGG AGAGAAACGT GGTGTGGACA AGTCTCTCAT TGCTGTTTAC 
AGGAGGAAGT GTCTCACTGC ACTAAATGAG AGCGCATCGC AGCTATTTTG CAGCGGTGAG 
GAGGCCAAAG TAGAAGAGGG TATTGCCATC ATGGATGAAG CCGTCATCCC CTGCCTTCAC 
CTGATGAACC GGGACTCGAC TTTACCCCAG GAAGACCGAG ATGCAATGGA GGCCATCCGC 
AGCCACTGGT GCTGCTGCCT GGGCCGAGAC ATGGATCAGT CATTGCAGGT GAAGCTCGGC 
GAGTTGCTTC CCAGAGTTCT GGACGGTTCA GCTGACACAG TAGTGCTTAA AGACCCACCC 
AAAGTCCACG TCACCCAGGC CTACGACCTG TGCAGTCGCC TGGCTGCTGT TATGGAATCC 
ATCCACAACA CATCAGTGGT CATTGTGAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp28 ( XM_023153118.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_023008886.1 Maylandia zebra ubiquitin specific peptidase 28 (usp28), transcript variant X2, mRNA.

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CloneID	OMa313080
Clone ID Related Accession (Same CDS sequence)	XM_023153118.1
Accession Version	XM_023153118.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-29
Organism	Maylandia zebra(zebra mbuna)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014444930.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTCGT CTGCAATGAG AGCCGATCAG TCTGAAAATG GAGAAAACTC AACCAACTCG 
AGTGAAATGC TGATCAGCCA GCTGAGGGAG ATCACTGGCA TCCAGGACCA ACAGATCCTC 
TACAGAGCCC TGAATGTCAG TCAGGGGGAC ATTGGACATG CTGTTGGACT GCTGACAACC 
CAGACTGCAG AGGTTCAGGA CCCTGGAGAA CCACAGGAGT CAGGGACATC TGGAGAGGCC 
TGGGAGGGCC AGAAAGGGCT TCCTAAAGAT GAACTGCAGA CCGCTATAGA ACTCAGCCTG 
CAGGAGTCCC ACAAAGCCCA GGAAGAGGAG CGAGAATTTC ATAGGGCTCT GGAAGCCAGT 
GCAGAGGAGA ATGCTGCGAG AATAAAGAGG AAGAGATGTG AAGCCCAGAG TGAAATGTGT 
AGCCCTGCAG AGTGGATCCG TCAGGAAGAT TGGCCTGTGG GCATTCGTAA TGTAGGAAAC 
ACCTGCTGGT TCAGTGCTGT CATCCAGTCA CTCTTCCACC TGCCTGTGTT CAGGAGACTG 
GTTCTCAACT ACCATCTGTC TGAGAAAATA CTGGAGAAGT GTAAAAGCCA TTCAGACAAG 
AGGAACATAG CCTTCATGCA GGAGCTGCGC TGCCTGTTTG CCCTCATGGT GGGCTCTACC 
CGCAGGTTTG TGGATCCTTC TGCTGCTGTG GAGTTATTGC GTGACGCCTT CAGGACCAAT 
GAGGCCCAAC AGGATGTCAG TGAGTTTTCC CACAAACTGC TTGACTGGCT CGAGGATGCA 
TTTCAGCTGG CTGCCAGTGG AAAAAATGCA GAAGATAAAC AACATAACCC CATGGTCCAG 
CTCTTCTATG GTACCTTTGT GACAGAAAGA AGACACGAGG GTAAGATGGT ATATAATATT 
GAGCAGTTTG GCCAGTACCC CCTGCAAGTC AATGGCTTCA ACAATCTTGA TGAATGCTTA 
GAAGGTGCTA TGGTAGAGAA GGAGATTGAG TCGGTTCATT CAGAGCATCA TGTCACATCT 
GGACGTGAGA GATGGTTCAA AAAGCTACCA CCAGTCTTGA CATTTGAACT TTCGAGATTT 
GAATTCAACA CCCAGCTTGG GCGCCCTGAG AAGATACATA AGAAACTGGA GTTTCCTCAA 
ATCATTTATA TGGACAGATA TCTTCACAAA AACATTGAGC AGACCCATGA GAGGAGAGGA 
GAAGTAAAGA AACTAAAGGA GCAACTGGCA GTCCTTCAAC AGAAACTTGA GTGTTATAAA 
AACTATGGCT CTGGACCTAC AAAGTACCCT CTAGCTGACA TGCTCCAGTT TGTGCTGGAG 
TTTGCTACCA CCAAGCCGAC TAGCGTTTCC CCAGCTGAAG ACTCGAGACA GGCCGCATCG 
TCACCGACTC CTCCAAGTGC CCCCCTCAGT GAAGCCATTG CCAAAGAAAG CAGTGATCCC 
GGAGAATCAG TTTCATCAGA GAGCCTGGGT TCCAGTGTTT CCAGTTGCCA GAGGACCCCC 
ATATACAAGC CCTTCACTCA GTGCAGACAA CCTATTGACT GCCCACCACA CCCTGCCCCC 
CACAGCATCA CAGAAGAAGA GCTGCACTTT GCTAAAACCT GTCTTCAGCG TTGGAGGACT 
GAAGTTGAGA ATGACATAAA TGAACTAAAG GCCAGCATTG ACAGGCTCAC TCAGACGCTC 
GAGGGCATGT ACTCAGACAA CAGTCTCTGC CAGGCCCCCT ACAGACTCCA TGCAGTGCTT 
GTCCATGAAG GCCAGGCATC AGCTGGTCAT TACTGGGCTT ACATCTATGA CCATGCTAAC 
CAGCGCTGGC TGAAGTACAA TGACATAAGT GTCACTGAGT CATCGTGGGA TGAGCTGGAG 
CGAGACTCCT TTGGAGGCAT GACCAACGCA AGTGCCTACT GCCTAATGTA CATTGACGAC 
AGGCTACCTC AGCTCATCAC AGAAGACACA GATGATGAGA CGGGCCAGGT GCTCCATGGC 
ATGGACTCTC TGCCACCCAT CCTCAGGCGC TTTGTCCACG AAGATAACCG CTGGTTCCAG 
CAGGAGCTCA GCGAATGGGA GGAGCAGTTC TGCCAGACAG CAACCCCGCA GGAAGAGTCC 
ACCGCCTCTG CAGAGCCCAC AAGTCCCAGT CTGGATAACA TACAGCAGTC ACCTGTTGAG 
CCAGCACCCC AGTCAGGATC CACTACTGAA GAGGTGGATG AAGGAGTTGC TCCAGAGCCA 
CAGCCAGACC AAGAAGCAGA GAAAGAGCCA GAACCTGATC TCAGAAAAGA GCCAGAAGCG 
CAAACAGATT TATCTCCCCC ACCACCAGAG GGCCCTGGCT GCCAGCCAGG TGATACCAGC 
ACTCCAACAG TCACTGACAC CACGGGGGTC AGCCAGAGCT CTGATTCAGA GGAGAAGGAG 
CCGCAGAATC AGATCCCTGG CCCTGATGTA GAGCTTTGCG ATCAGACACC ATCTGACATC 
CACGTAGAGA AGGATAACCC AGAGGTGGCC GGCCTCGTCT CTGAAACTGA AGAAGAGCCC 
CAGGCTTCTG GAGAGGCTCC TGAACCTATC GCAGAGCAAA GCAATCAGGA GCAGCAGCAG 
CAGCAGCAGG AGGTTCCAGC CAGACCACGA CAGACTGAGA ATGAGGTCTC AGAAGTGGAG 
ATCCCCAATG TGGGCCGGAT CATGGTGAGG GCGGATGCTG ACGGATACAA TGAAGAGATG 
ATGCTAACTC CAGCTATGCA GGGTGTCATT CTAGCCATAG CCAAGGCCAG ACAAATTTTT 
GATAAGGAGG GTCCTGAGGC TGGCCTCATC AAGGCTTTCC ATGAAGAGTA TTCGCGCTTG 
TATGAGCTCT CTGAAGAGGA GACCACACCA CAGGAGGATG CACGTCTCCA GCACGCTCTG 
GTCTACTTCT TCCAGAACAA GGCACCCAAG CGTGTCATTG AGAGGACGCT GCTTGAGCAG 
TTTACTGACC GCAACCTCAG TTTTGATGAG AGAGCCATCA GTATCATGAG GGAAGCCCGG 
GCCAAACTTC GTCTCATTAA GCCAGAAGAC ATGGACATGG AAGAATACAT GCAGTGGCAT 
GACGATTACA GGCTGTTCAG GACGGTCTTT GTCTATCTGT TGACTGGGCT GGAGCATTAC 
CAGCACGGGA AGATGCGTGA TGCACTGAAT TACTTGGCCC ACGCCTATGA GACCAACAGC 
ACCCTGCTGC GAAACGGAGA GAAACGTGGT GTGGACAAGT CTCTCATTGC TGTTTACAGG 
AGGAAGTGTC TCACTGCACT AAATGAGAGC GCATCGCAGC TATTTTGCAG CGGTGAGGAG 
GCCAAAGTAG AAGAGGGTAT TGCCATCATG GATGAAGCCG TCATCCCCTG CCTTCACCTG 
ATGAACCGGG ACTCGACTTT ACCCCAGGAA GACCGAGATG CAATGGAGGC CATCCGCAGC 
CACTGGTGCT GCTGCCTGGG CCGAGACATG GATCAGTCAT TGCAGGTGAA GCTCGGCGAG 
TTGCTTCCCA GAGTTCTGGA CGGTTCAGCT GACACAGTAG TGCTTAAAGA CCCACCCAAA 
GTCCACGTCA CCCAGGCCTA CGACCTGTGC AGTCGCCTGG CTGCTGTTAT GGAATCCATC 
CACAACACAT CAGTGGTCAT TGTGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023008886.1
CDS	89..3718
Translation	MFSSAMRADQSENGENSTNSSEMLISQLREITGIQDQQILYRALNVSQGDIGHAVGLLTTQTAEVQDPGEPQESGTSGEAWEGQKGLPKDELQTAIELSLQESHKAQEEEREFHRALEASAEENAARIKRKRCEAQSEMCSPAEWIRQEDWPVGIRNVGNTCWFSAVIQSLFHLPVFRRLVLNYHLSEKILEKCKSHSDKRNIAFMQELRCLFALMVGSTRRFVDPSAAVELLRDAFRTNEAQQDVSEFSHKLLDWLEDAFQLAASGKNAEDKQHNPMVQLFYGTFVTERRHEGKMVYNIEQFGQYPLQVNGFNNLDECLEGAMVEKEIESVHSEHHVTSGRERWFKKLPPVLTFELSRFEFNTQLGRPEKIHKKLEFPQIIYMDRYLHKNIEQTHERRGEVKKLKEQLAVLQQKLECYKNYGSGPTKYPLADMLQFVLEFATTKPTSVSPAEDSRQAASSPTPPSAPLSEAIAKESSDPGESVSSESLGSSVSSCQRTPIYKPFTQCRQPIDCPPHPAPHSITEEELHFAKTCLQRWRTEVENDINELKASIDRLTQTLEGMYSDNSLCQAPYRLHAVLVHEGQASAGHYWAYIYDHANQRWLKYNDISVTESSWDELERDSFGGMTNASAYCLMYIDDRLPQLITEDTDDETGQVLHGMDSLPPILRRFVHEDNRWFQQELSEWEEQFCQTATPQEESTASAEPTSPSLDNIQQSPVEPAPQSGSTTEEVDEGVAPEPQPDQEAEKEPEPDLRKEPEAQTDLSPPPPEGPGCQPGDTSTPTVTDTTGVSQSSDSEEKEPQNQIPGPDVELCDQTPSDIHVEKDNPEVAGLVSETEEEPQASGEAPEPIAEQSNQEQQQQQQEVPARPRQTENEVSEVEIPNVGRIMVRADADGYNEEMMLTPAMQGVILAIAKARQIFDKEGPEAGLIKAFHEEYSRLYELSEEETTPQEDARLQHALVYFFQNKAPKRVIERTLLEQFTDRNLSFDERAISIMREARAKLRLIKPEDMDMEEYMQWHDDYRLFRTVFVYLLTGLEHYQHGKMRDALNYLAHAYETNSTLLRNGEKRGVDKSLIAVYRRKCLTALNESASQLFCSGEEAKVEEGIAIMDEAVIPCLHLMNRDSTLPQEDRDAMEAIRSHWCCCLGRDMDQSLQVKLGELLPRVLDGSADTVVLKDPPKVHVTQAYDLCSRLAAVMESIHNTSVVIVK

Target ORF information:

RefSeq Version	XM_023153118.1
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra ubiquitin specific peptidase 28 (usp28), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023153118.1

ORF Insert Sequence:

ATGTTTTCGT CTGCAATGAG AGCCGATCAG TCTGAAAATG GAGAAAACTC AACCAACTCG 
AGTGAAATGC TGATCAGCCA GCTGAGGGAG ATCACTGGCA TCCAGGACCA ACAGATCCTC 
TACAGAGCCC TGAATGTCAG TCAGGGGGAC ATTGGACATG CTGTTGGACT GCTGACAACC 
CAGACTGCAG AGGTTCAGGA CCCTGGAGAA CCACAGGAGT CAGGGACATC TGGAGAGGCC 
TGGGAGGGCC AGAAAGGGCT TCCTAAAGAT GAACTGCAGA CCGCTATAGA ACTCAGCCTG 
CAGGAGTCCC ACAAAGCCCA GGAAGAGGAG CGAGAATTTC ATAGGGCTCT GGAAGCCAGT 
GCAGAGGAGA ATGCTGCGAG AATAAAGAGG AAGAGATGTG AAGCCCAGAG TGAAATGTGT 
AGCCCTGCAG AGTGGATCCG TCAGGAAGAT TGGCCTGTGG GCATTCGTAA TGTAGGAAAC 
ACCTGCTGGT TCAGTGCTGT CATCCAGTCA CTCTTCCACC TGCCTGTGTT CAGGAGACTG 
GTTCTCAACT ACCATCTGTC TGAGAAAATA CTGGAGAAGT GTAAAAGCCA TTCAGACAAG 
AGGAACATAG CCTTCATGCA GGAGCTGCGC TGCCTGTTTG CCCTCATGGT GGGCTCTACC 
CGCAGGTTTG TGGATCCTTC TGCTGCTGTG GAGTTATTGC GTGACGCCTT CAGGACCAAT 
GAGGCCCAAC AGGATGTCAG TGAGTTTTCC CACAAACTGC TTGACTGGCT CGAGGATGCA 
TTTCAGCTGG CTGCCAGTGG AAAAAATGCA GAAGATAAAC AACATAACCC CATGGTCCAG 
CTCTTCTATG GTACCTTTGT GACAGAAAGA AGACACGAGG GTAAGATGGT ATATAATATT 
GAGCAGTTTG GCCAGTACCC CCTGCAAGTC AATGGCTTCA ACAATCTTGA TGAATGCTTA 
GAAGGTGCTA TGGTAGAGAA GGAGATTGAG TCGGTTCATT CAGAGCATCA TGTCACATCT 
GGACGTGAGA GATGGTTCAA AAAGCTACCA CCAGTCTTGA CATTTGAACT TTCGAGATTT 
GAATTCAACA CCCAGCTTGG GCGCCCTGAG AAGATACATA AGAAACTGGA GTTTCCTCAA 
ATCATTTATA TGGACAGATA TCTTCACAAA AACATTGAGC AGACCCATGA GAGGAGAGGA 
GAAGTAAAGA AACTAAAGGA GCAACTGGCA GTCCTTCAAC AGAAACTTGA GTGTTATAAA 
AACTATGGCT CTGGACCTAC AAAGTACCCT CTAGCTGACA TGCTCCAGTT TGTGCTGGAG 
TTTGCTACCA CCAAGCCGAC TAGCGTTTCC CCAGCTGAAG ACTCGAGACA GGCCGCATCG 
TCACCGACTC CTCCAAGTGC CCCCCTCAGT GAAGCCATTG CCAAAGAAAG CAGTGATCCC 
GGAGAATCAG TTTCATCAGA GAGCCTGGGT TCCAGTGTTT CCAGTTGCCA GAGGACCCCC 
ATATACAAGC CCTTCACTCA GTGCAGACAA CCTATTGACT GCCCACCACA CCCTGCCCCC 
CACAGCATCA CAGAAGAAGA GCTGCACTTT GCTAAAACCT GTCTTCAGCG TTGGAGGACT 
GAAGTTGAGA ATGACATAAA TGAACTAAAG GCCAGCATTG ACAGGCTCAC TCAGACGCTC 
GAGGGCATGT ACTCAGACAA CAGTCTCTGC CAGGCCCCCT ACAGACTCCA TGCAGTGCTT 
GTCCATGAAG GCCAGGCATC AGCTGGTCAT TACTGGGCTT ACATCTATGA CCATGCTAAC 
CAGCGCTGGC TGAAGTACAA TGACATAAGT GTCACTGAGT CATCGTGGGA TGAGCTGGAG 
CGAGACTCCT TTGGAGGCAT GACCAACGCA AGTGCCTACT GCCTAATGTA CATTGACGAC 
AGGCTACCTC AGCTCATCAC AGAAGACACA GATGATGAGA CGGGCCAGGT GCTCCATGGC 
ATGGACTCTC TGCCACCCAT CCTCAGGCGC TTTGTCCACG AAGATAACCG CTGGTTCCAG 
CAGGAGCTCA GCGAATGGGA GGAGCAGTTC TGCCAGACAG CAACCCCGCA GGAAGAGTCC 
ACCGCCTCTG CAGAGCCCAC AAGTCCCAGT CTGGATAACA TACAGCAGTC ACCTGTTGAG 
CCAGCACCCC AGTCAGGATC CACTACTGAA GAGGTGGATG AAGGAGTTGC TCCAGAGCCA 
CAGCCAGACC AAGAAGCAGA GAAAGAGCCA GAACCTGATC TCAGAAAAGA GCCAGAAGCG 
CAAACAGATT TATCTCCCCC ACCACCAGAG GGCCCTGGCT GCCAGCCAGG TGATACCAGC 
ACTCCAACAG TCACTGACAC CACGGGGGTC AGCCAGAGCT CTGATTCAGA GGAGAAGGAG 
CCGCAGAATC AGATCCCTGG CCCTGATGTA GAGCTTTGCG ATCAGACACC ATCTGACATC 
CACGTAGAGA AGGATAACCC AGAGGTGGCC GGCCTCGTCT CTGAAACTGA AGAAGAGCCC 
CAGGCTTCTG GAGAGGCTCC TGAACCTATC GCAGAGCAAA GCAATCAGGA GCAGCAGCAG 
CAGCAGCAGG AGGTTCCAGC CAGACCACGA CAGACTGAGA ATGAGGTCTC AGAAGTGGAG 
ATCCCCAATG TGGGCCGGAT CATGGTGAGG GCGGATGCTG ACGGATACAA TGAAGAGATG 
ATGCTAACTC CAGCTATGCA GGGTGTCATT CTAGCCATAG CCAAGGCCAG ACAAATTTTT 
GATAAGGAGG GTCCTGAGGC TGGCCTCATC AAGGCTTTCC ATGAAGAGTA TTCGCGCTTG 
TATGAGCTCT CTGAAGAGGA GACCACACCA CAGGAGGATG CACGTCTCCA GCACGCTCTG 
GTCTACTTCT TCCAGAACAA GGCACCCAAG CGTGTCATTG AGAGGACGCT GCTTGAGCAG 
TTTACTGACC GCAACCTCAG TTTTGATGAG AGAGCCATCA GTATCATGAG GGAAGCCCGG 
GCCAAACTTC GTCTCATTAA GCCAGAAGAC ATGGACATGG AAGAATACAT GCAGTGGCAT 
GACGATTACA GGCTGTTCAG GACGGTCTTT GTCTATCTGT TGACTGGGCT GGAGCATTAC 
CAGCACGGGA AGATGCGTGA TGCACTGAAT TACTTGGCCC ACGCCTATGA GACCAACAGC 
ACCCTGCTGC GAAACGGAGA GAAACGTGGT GTGGACAAGT CTCTCATTGC TGTTTACAGG 
AGGAAGTGTC TCACTGCACT AAATGAGAGC GCATCGCAGC TATTTTGCAG CGGTGAGGAG 
GCCAAAGTAG AAGAGGGTAT TGCCATCATG GATGAAGCCG TCATCCCCTG CCTTCACCTG 
ATGAACCGGG ACTCGACTTT ACCCCAGGAA GACCGAGATG CAATGGAGGC CATCCGCAGC 
CACTGGTGCT GCTGCCTGGG CCGAGACATG GATCAGTCAT TGCAGGTGAA GCTCGGCGAG 
TTGCTTCCCA GAGTTCTGGA CGGTTCAGCT GACACAGTAG TGCTTAAAGA CCCACCCAAA 
GTCCACGTCA CCCAGGCCTA CGACCTGTGC AGTCGCCTGG CTGCTGTTAT GGAATCCATC 
CACAACACAT CAGTGGTCAT TGTGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp28 ( XM_023153119.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_023008887.1 Maylandia zebra ubiquitin specific peptidase 28 (usp28), transcript variant X3, mRNA.

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CloneID	OMa313081
Clone ID Related Accession (Same CDS sequence)	XM_023153119.1
Accession Version	XM_023153119.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-29
Organism	Maylandia zebra(zebra mbuna)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014444930.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTCGT CTGCAATGAG AGCCGATCAG TCTGAAAATG GAGAAAACTC AACCAACTCG 
CAGAGTGAAA TGCTGATCAG CCAGCTGAGG GAGATCACTG GCATCCAGGA CCAACAGATC 
CTCTACAGAG CCCTGAATGT CAGTCAGGGG GACATTGGAC ATGCTGTTGG ACTGCTGACA 
ACCCAGACTG CAGAGGTTCA GGACCCTGGA GAACCACAGG AGTCAGGGAC ATCTGGAGAG 
GCCTGGGAGG GCCAGAAAGG GCTTCCTAAA GATGAACTGC AGACCGCTAT AGAACTCAGC 
CTGCAGGAGT CCCACAAAGC CCAGGAAGAG GAGCGAGAAT TTCATAGGGC TCTGGAAGCC 
AGTGCAGAGG AGAATGCTGC GAGAATAAAG AGGAAGAGAT GTGAAGCCCA GAGTGAAATG 
TGTAGCCCTG CAGAGTGGAT CCGTCAGGAA GATTGGCCTG TGGGCATTCG TAATGTAGGA 
AACACCTGCT GGTTCAGTGC TGTCATCCAG TCACTCTTCC ACCTGCCTGT GTTCAGGAGA 
CTGGTTCTCA ACTACCATCT GTCTGAGAAA ATACTGGAGA AGTGTAAAAG CCATTCAGAC 
AAGAGGAACA TAGCCTTCAT GCAGGAGCTG CGCTGCCTGT TTGCCCTCAT GGTGGGCTCT 
ACCCGCAGGT TTGTGGATCC TTCTGCTGCT GTGGAGTTAT TGCGTGACGC CTTCAGGACC 
AATGAGGCCC AACAGGATGT CAGTGAGTTT TCCCACAAAC TGCTTGACTG GCTCGAGGAT 
GCATTTCAGC TGGCTGCCAG TGGAAAAAAT GCAGAAGATA AACAACATAA CCCCATGGTC 
CAGCTCTTCT ATGGTACCTT TGTGACAGAA AGAAGACACG AGGGTAAGAT GGTATATAAT 
ATTGAGCAGT TTGGCCAGTA CCCCCTGCAA GTCAATGGCT TCAACAATCT TGATGAATGC 
TTAGAAGGTG CTATGGTAGA GAAGGAGATT GAGTCGGTTC ATTCAGAGCA TCATGTCACA 
TCTGGACGTG AGAGATGGTT CAAAAAGCTA CCACCAGTCT TGACATTTGA ACTTTCGAGA 
TTTGAATTCA ACACCCAGCT TGGGCGCCCT GAGAAGATAC ATAAGAAACT GGAGTTTCCT 
CAAATCATTT ATATGGACAG ATATCTTCAC AAAAACATTG AGCAGACCCA TGAGAGGAGA 
GGAGAAGTAA AGAAACTAAA GGAGCAACTG GCAGTCCTTC AACAGAAACT TGAGTGTTAT 
AAAAACTATG GCTCTGGACC TACAAAGTAC CCTCTAGCTG ACATGCTCCA GTTTGTGCTG 
GAGTTTGCTA CCACCAAGCC GACTAGCGTT TCCCCAGCTG AAGACTCGAG ACAGGCCGCA 
TCGTCACCGA CTCCTCCAAG TGCCCCCCTC AGTGAAGCCA TTGCCAAAGA AAGCAGTGAT 
CCCGGAGAAT CAGTTTCATC AGAGAGCCTG GGTTCCAGTG TTTCCAGTTG CCAGAGGACC 
CCCATATACA AGCCCTTCAC TCAGTGCAGA CAACCTATTG ACTGCCCACC ACACCCTGCC 
CCCCACAGCA TCACAGAAGA AGAGCTGCAC TTTGCTAAAA CCTGTCTTCA GCGTTGGAGG 
ACTGAAGTTG AGAATGACAT AAATGAACTA AAGGCCAGCA TTGACAGGCT CACTCAGACG 
CTCGAGGGCA TGTACTCAGA CAACAGTCTC TGCCAGGCCC CCTACAGACT CCATGCAGTG 
CTTGTCCATG AAGGCCAGGC ATCAGCTGGT CATTACTGGG CTTACATCTA TGACCATGCT 
AACCAGCGCT GGCTGAAGTA CAATGACATA AGTGTCACTG AGTCATCGTG GGATGAGCTG 
GAGCGAGACT CCTTTGGAGG CATGACCAAC GCAAGTGCCT ACTGCCTAAT GTACATTGAC 
GACAGGCTAC CTCAGCTCAT CACAGACACA GATGATGAGA CGGGCCAGGT GCTCCATGGC 
ATGGACTCTC TGCCACCCAT CCTCAGGCGC TTTGTCCACG AAGATAACCG CTGGTTCCAG 
CAGGAGCTCA GCGAATGGGA GGAGCAGTTC TGCCAGACAG CAACCCCGCA GGAAGAGTCC 
ACCGCCTCTG CAGAGCCCAC AAGTCCCAGT CTGGATAACA TACAGCAGTC ACCTGTTGAG 
CCAGCACCCC AGTCAGGATC CACTACTGAA GAGGTGGATG AAGGAGTTGC TCCAGAGCCA 
CAGCCAGACC AAGAAGCAGA GAAAGAGCCA GAACCTGATC TCAGAAAAGA GCCAGAAGCG 
CAAACAGATT TATCTCCCCC ACCACCAGAG GGCCCTGGCT GCCAGCCAGG TGATACCAGC 
ACTCCAACAG TCACTGACAC CACGGGGGTC AGCCAGAGCT CTGATTCAGA GGAGAAGGAG 
CCGCAGAATC AGATCCCTGG CCCTGATGTA GAGCTTTGCG ATCAGACACC ATCTGACATC 
CACGTAGAGA AGGATAACCC AGAGGTGGCC GGCCTCGTCT CTGAAACTGA AGAAGAGCCC 
CAGGCTTCTG GAGAGGCTCC TGAACCTATC GCAGAGCAAA GCAATCAGGA GCAGCAGCAG 
CAGCAGCAGG AGGTTCCAGC CAGACCACGA CAGACTGAGA ATGAGGTCTC AGAAGTGGAG 
ATCCCCAATG TGGGCCGGAT CATGGTGAGG GCGGATGCTG ACGGATACAA TGAAGAGATG 
ATGCTAACTC CAGCTATGCA GGGTGTCATT CTAGCCATAG CCAAGGCCAG ACAAATTTTT 
GATAAGGAGG GTCCTGAGGC TGGCCTCATC AAGGCTTTCC ATGAAGAGTA TTCGCGCTTG 
TATGAGCTCT CTGAAGAGGA GACCACACCA CAGGAGGATG CACGTCTCCA GCACGCTCTG 
GTCTACTTCT TCCAGAACAA GGCACCCAAG CGTGTCATTG AGAGGACGCT GCTTGAGCAG 
TTTACTGACC GCAACCTCAG TTTTGATGAG AGAGCCATCA GTATCATGAG GGAAGCCCGG 
GCCAAACTTC GTCTCATTAA GCCAGAAGAC ATGGACATGG AAGAATACAT GCAGTGGCAT 
GACGATTACA GGCTGTTCAG GACGGTCTTT GTCTATCTGT TGACTGGGCT GGAGCATTAC 
CAGCACGGGA AGATGCGTGA TGCACTGAAT TACTTGGCCC ACGCCTATGA GACCAACAGC 
ACCCTGCTGC GAAACGGAGA GAAACGTGGT GTGGACAAGT CTCTCATTGC TGTTTACAGG 
AGGAAGTGTC TCACTGCACT AAATGAGAGC GCATCGCAGC TATTTTGCAG CGGTGAGGAG 
GCCAAAGTAG AAGAGGGTAT TGCCATCATG GATGAAGCCG TCATCCCCTG CCTTCACCTG 
ATGAACCGGG ACTCGACTTT ACCCCAGGAA GACCGAGATG CAATGGAGGC CATCCGCAGC 
CACTGGTGCT GCTGCCTGGG CCGAGACATG GATCAGTCAT TGCAGGTGAA GCTCGGCGAG 
TTGCTTCCCA GAGTTCTGGA CGGTTCAGCT GACACAGTAG TGCTTAAAGA CCCACCCAAA 
GTCCACGTCA CCCAGGCCTA CGACCTGTGC AGTCGCCTGG CTGCTGTTAT GGAATCCATC 
CACAACACAT CAGTGGTCAT TGTGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023008887.1
CDS	89..3718
Translation	MFSSAMRADQSENGENSTNSQSEMLISQLREITGIQDQQILYRALNVSQGDIGHAVGLLTTQTAEVQDPGEPQESGTSGEAWEGQKGLPKDELQTAIELSLQESHKAQEEEREFHRALEASAEENAARIKRKRCEAQSEMCSPAEWIRQEDWPVGIRNVGNTCWFSAVIQSLFHLPVFRRLVLNYHLSEKILEKCKSHSDKRNIAFMQELRCLFALMVGSTRRFVDPSAAVELLRDAFRTNEAQQDVSEFSHKLLDWLEDAFQLAASGKNAEDKQHNPMVQLFYGTFVTERRHEGKMVYNIEQFGQYPLQVNGFNNLDECLEGAMVEKEIESVHSEHHVTSGRERWFKKLPPVLTFELSRFEFNTQLGRPEKIHKKLEFPQIIYMDRYLHKNIEQTHERRGEVKKLKEQLAVLQQKLECYKNYGSGPTKYPLADMLQFVLEFATTKPTSVSPAEDSRQAASSPTPPSAPLSEAIAKESSDPGESVSSESLGSSVSSCQRTPIYKPFTQCRQPIDCPPHPAPHSITEEELHFAKTCLQRWRTEVENDINELKASIDRLTQTLEGMYSDNSLCQAPYRLHAVLVHEGQASAGHYWAYIYDHANQRWLKYNDISVTESSWDELERDSFGGMTNASAYCLMYIDDRLPQLITDTDDETGQVLHGMDSLPPILRRFVHEDNRWFQQELSEWEEQFCQTATPQEESTASAEPTSPSLDNIQQSPVEPAPQSGSTTEEVDEGVAPEPQPDQEAEKEPEPDLRKEPEAQTDLSPPPPEGPGCQPGDTSTPTVTDTTGVSQSSDSEEKEPQNQIPGPDVELCDQTPSDIHVEKDNPEVAGLVSETEEEPQASGEAPEPIAEQSNQEQQQQQQEVPARPRQTENEVSEVEIPNVGRIMVRADADGYNEEMMLTPAMQGVILAIAKARQIFDKEGPEAGLIKAFHEEYSRLYELSEEETTPQEDARLQHALVYFFQNKAPKRVIERTLLEQFTDRNLSFDERAISIMREARAKLRLIKPEDMDMEEYMQWHDDYRLFRTVFVYLLTGLEHYQHGKMRDALNYLAHAYETNSTLLRNGEKRGVDKSLIAVYRRKCLTALNESASQLFCSGEEAKVEEGIAIMDEAVIPCLHLMNRDSTLPQEDRDAMEAIRSHWCCCLGRDMDQSLQVKLGELLPRVLDGSADTVVLKDPPKVHVTQAYDLCSRLAAVMESIHNTSVVIVK

Target ORF information:

RefSeq Version	XM_023153119.1
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra ubiquitin specific peptidase 28 (usp28), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023153119.1

ORF Insert Sequence:

ATGTTTTCGT CTGCAATGAG AGCCGATCAG TCTGAAAATG GAGAAAACTC AACCAACTCG 
CAGAGTGAAA TGCTGATCAG CCAGCTGAGG GAGATCACTG GCATCCAGGA CCAACAGATC 
CTCTACAGAG CCCTGAATGT CAGTCAGGGG GACATTGGAC ATGCTGTTGG ACTGCTGACA 
ACCCAGACTG CAGAGGTTCA GGACCCTGGA GAACCACAGG AGTCAGGGAC ATCTGGAGAG 
GCCTGGGAGG GCCAGAAAGG GCTTCCTAAA GATGAACTGC AGACCGCTAT AGAACTCAGC 
CTGCAGGAGT CCCACAAAGC CCAGGAAGAG GAGCGAGAAT TTCATAGGGC TCTGGAAGCC 
AGTGCAGAGG AGAATGCTGC GAGAATAAAG AGGAAGAGAT GTGAAGCCCA GAGTGAAATG 
TGTAGCCCTG CAGAGTGGAT CCGTCAGGAA GATTGGCCTG TGGGCATTCG TAATGTAGGA 
AACACCTGCT GGTTCAGTGC TGTCATCCAG TCACTCTTCC ACCTGCCTGT GTTCAGGAGA 
CTGGTTCTCA ACTACCATCT GTCTGAGAAA ATACTGGAGA AGTGTAAAAG CCATTCAGAC 
AAGAGGAACA TAGCCTTCAT GCAGGAGCTG CGCTGCCTGT TTGCCCTCAT GGTGGGCTCT 
ACCCGCAGGT TTGTGGATCC TTCTGCTGCT GTGGAGTTAT TGCGTGACGC CTTCAGGACC 
AATGAGGCCC AACAGGATGT CAGTGAGTTT TCCCACAAAC TGCTTGACTG GCTCGAGGAT 
GCATTTCAGC TGGCTGCCAG TGGAAAAAAT GCAGAAGATA AACAACATAA CCCCATGGTC 
CAGCTCTTCT ATGGTACCTT TGTGACAGAA AGAAGACACG AGGGTAAGAT GGTATATAAT 
ATTGAGCAGT TTGGCCAGTA CCCCCTGCAA GTCAATGGCT TCAACAATCT TGATGAATGC 
TTAGAAGGTG CTATGGTAGA GAAGGAGATT GAGTCGGTTC ATTCAGAGCA TCATGTCACA 
TCTGGACGTG AGAGATGGTT CAAAAAGCTA CCACCAGTCT TGACATTTGA ACTTTCGAGA 
TTTGAATTCA ACACCCAGCT TGGGCGCCCT GAGAAGATAC ATAAGAAACT GGAGTTTCCT 
CAAATCATTT ATATGGACAG ATATCTTCAC AAAAACATTG AGCAGACCCA TGAGAGGAGA 
GGAGAAGTAA AGAAACTAAA GGAGCAACTG GCAGTCCTTC AACAGAAACT TGAGTGTTAT 
AAAAACTATG GCTCTGGACC TACAAAGTAC CCTCTAGCTG ACATGCTCCA GTTTGTGCTG 
GAGTTTGCTA CCACCAAGCC GACTAGCGTT TCCCCAGCTG AAGACTCGAG ACAGGCCGCA 
TCGTCACCGA CTCCTCCAAG TGCCCCCCTC AGTGAAGCCA TTGCCAAAGA AAGCAGTGAT 
CCCGGAGAAT CAGTTTCATC AGAGAGCCTG GGTTCCAGTG TTTCCAGTTG CCAGAGGACC 
CCCATATACA AGCCCTTCAC TCAGTGCAGA CAACCTATTG ACTGCCCACC ACACCCTGCC 
CCCCACAGCA TCACAGAAGA AGAGCTGCAC TTTGCTAAAA CCTGTCTTCA GCGTTGGAGG 
ACTGAAGTTG AGAATGACAT AAATGAACTA AAGGCCAGCA TTGACAGGCT CACTCAGACG 
CTCGAGGGCA TGTACTCAGA CAACAGTCTC TGCCAGGCCC CCTACAGACT CCATGCAGTG 
CTTGTCCATG AAGGCCAGGC ATCAGCTGGT CATTACTGGG CTTACATCTA TGACCATGCT 
AACCAGCGCT GGCTGAAGTA CAATGACATA AGTGTCACTG AGTCATCGTG GGATGAGCTG 
GAGCGAGACT CCTTTGGAGG CATGACCAAC GCAAGTGCCT ACTGCCTAAT GTACATTGAC 
GACAGGCTAC CTCAGCTCAT CACAGACACA GATGATGAGA CGGGCCAGGT GCTCCATGGC 
ATGGACTCTC TGCCACCCAT CCTCAGGCGC TTTGTCCACG AAGATAACCG CTGGTTCCAG 
CAGGAGCTCA GCGAATGGGA GGAGCAGTTC TGCCAGACAG CAACCCCGCA GGAAGAGTCC 
ACCGCCTCTG CAGAGCCCAC AAGTCCCAGT CTGGATAACA TACAGCAGTC ACCTGTTGAG 
CCAGCACCCC AGTCAGGATC CACTACTGAA GAGGTGGATG AAGGAGTTGC TCCAGAGCCA 
CAGCCAGACC AAGAAGCAGA GAAAGAGCCA GAACCTGATC TCAGAAAAGA GCCAGAAGCG 
CAAACAGATT TATCTCCCCC ACCACCAGAG GGCCCTGGCT GCCAGCCAGG TGATACCAGC 
ACTCCAACAG TCACTGACAC CACGGGGGTC AGCCAGAGCT CTGATTCAGA GGAGAAGGAG 
CCGCAGAATC AGATCCCTGG CCCTGATGTA GAGCTTTGCG ATCAGACACC ATCTGACATC 
CACGTAGAGA AGGATAACCC AGAGGTGGCC GGCCTCGTCT CTGAAACTGA AGAAGAGCCC 
CAGGCTTCTG GAGAGGCTCC TGAACCTATC GCAGAGCAAA GCAATCAGGA GCAGCAGCAG 
CAGCAGCAGG AGGTTCCAGC CAGACCACGA CAGACTGAGA ATGAGGTCTC AGAAGTGGAG 
ATCCCCAATG TGGGCCGGAT CATGGTGAGG GCGGATGCTG ACGGATACAA TGAAGAGATG 
ATGCTAACTC CAGCTATGCA GGGTGTCATT CTAGCCATAG CCAAGGCCAG ACAAATTTTT 
GATAAGGAGG GTCCTGAGGC TGGCCTCATC AAGGCTTTCC ATGAAGAGTA TTCGCGCTTG 
TATGAGCTCT CTGAAGAGGA GACCACACCA CAGGAGGATG CACGTCTCCA GCACGCTCTG 
GTCTACTTCT TCCAGAACAA GGCACCCAAG CGTGTCATTG AGAGGACGCT GCTTGAGCAG 
TTTACTGACC GCAACCTCAG TTTTGATGAG AGAGCCATCA GTATCATGAG GGAAGCCCGG 
GCCAAACTTC GTCTCATTAA GCCAGAAGAC ATGGACATGG AAGAATACAT GCAGTGGCAT 
GACGATTACA GGCTGTTCAG GACGGTCTTT GTCTATCTGT TGACTGGGCT GGAGCATTAC 
CAGCACGGGA AGATGCGTGA TGCACTGAAT TACTTGGCCC ACGCCTATGA GACCAACAGC 
ACCCTGCTGC GAAACGGAGA GAAACGTGGT GTGGACAAGT CTCTCATTGC TGTTTACAGG 
AGGAAGTGTC TCACTGCACT AAATGAGAGC GCATCGCAGC TATTTTGCAG CGGTGAGGAG 
GCCAAAGTAG AAGAGGGTAT TGCCATCATG GATGAAGCCG TCATCCCCTG CCTTCACCTG 
ATGAACCGGG ACTCGACTTT ACCCCAGGAA GACCGAGATG CAATGGAGGC CATCCGCAGC 
CACTGGTGCT GCTGCCTGGG CCGAGACATG GATCAGTCAT TGCAGGTGAA GCTCGGCGAG 
TTGCTTCCCA GAGTTCTGGA CGGTTCAGCT GACACAGTAG TGCTTAAAGA CCCACCCAAA 
GTCCACGTCA CCCAGGCCTA CGACCTGTGC AGTCGCCTGG CTGCTGTTAT GGAATCCATC 
CACAACACAT CAGTGGTCAT TGTGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp28 ( XM_004573473.5 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004573530.2 Maylandia zebra ubiquitin specific peptidase 28 (usp28), transcript variant X1, mRNA.

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CloneID	OMa359731
Clone ID Related Accession (Same CDS sequence)	XM_004573473.5
Accession Version	XM_004573473.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-23
Organism	Maylandia zebra(zebra mbuna)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036789.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_004573473.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTCGT CTGCAATGAG AGCCGATCAG TCTGAAAATG GAGAAAACTC AACCAACTCG 
CAGAGTGAAA TGCTGATCAG CCAGCTGAGG GAGATCACTG GCATCCAGGA CCAACAGATC 
CTCTACAGAG CCCTGAATGT CAGTCAGGGG GACATTGGAC ATGCTGTTGG ACTGCTGACA 
ACCCAGACTG CAGAGGTTCA GGACCCTGGA GAACCACAGG AGTCAGGGAC ATCTGGAGAG 
GCCTGGGAGG GCCAGAAAGG GCTTCCTAAA GATGAACTGC AGACCGCTAT AGAACTCAGC 
CTGCAGGAGT CCCACAAAGC CCAGGAAGAG GAGCGAGAAT TTCATAGGGC TCTGGAAGCC 
AGTGCAGAGG AGAATGCTGC GAGAATAAAG AGGAAGAGAT GTGAAGCCCA GAGTGAAATG 
TGTAGCCCTG CAGAGTGGAT CCGTCAGGAA GATTGGCCTG TGGGCATTCG TAATGTAGGA 
AACACCTGCT GGTTCAGTGC TGTCATCCAG TCACTCTTCC ACCTGCCTGT GTTCAGGAGA 
CTGGTTCTCA ACTACCATCT GTCTGAGAAA ATACTGGAGA AGTGTAAAAG CCATTCAGAC 
AAGAGGAACA TAGCCTTCAT GCAGGAGCTG CGCTGCCTGT TTGCCCTCAT GGTGGGCTCT 
ACCCGCAGGT TTGTGGATCC TTCTGCTGCT GTGGAGTTAT TGCGTGACGC CTTCAGGACC 
AATGAGGCCC AACAGGATGT CAGTGAGTTT TCCCACAAAC TGCTTGACTG GCTCGAGGAT 
GCATTTCAGC TGGCTGCCAG TGGAAAAAAT GCAGAAGATA AACAACATAA CCCCATGGTC 
CAGCTCTTCT ATGGTACCTT TGTGACAGAA AGAAGACACG AGGGTAAGAT GGTATATAAT 
ATTGAGCAGT TTGGCCAGTA CCCCCTGCAA GTCAATGGCT TCAACAATCT TGATGAATGC 
TTAGAAGGTG CTATGGTAGA GAAGGAGATT GAGTCGGTTC ATTCAGAGCA TCATGTCACA 
TCTGGACGTG AGAGATGGTT CAAAAAGCTA CCACCAGTCT TGACATTTGA ACTTTCGAGA 
TTTGAATTCA ACACCCAGCT TGGGCGCCCT GAGAAGATAC ATAAGAAACT GGAGTTTCCT 
CAAATCATTT ATATGGACAG ATATCTTCAC AAAAACATTG AGCAGACCCA TGAGAGGAGA 
GGAGAAGTAA AGAAACTAAA GGAGCAACTG GCAGTCCTTC AACAGAAACT TGAGTGTTAT 
AAAAACTATG GCTCTGGACC TACAAAGTAC CCTCTAGCTG ACATGCTCCA GTTTGTGCTG 
GAGTTTGCTA CCACCAAGCC GACTAGCGTT TCCCCAGCTG AAGACTCGAG ACAGGCCGCA 
TCGTCACCGA CTCCTCCAAG TGCCCCCCTC AGTGAAGCCA TTGCCAAAGA AAGCAGTGAT 
CCCGGAGAAT CAGTTTCATC AGAGAGCCTG GGTTCCAGTG TTTCCAGTTG CCAGAGGACC 
CCCATATACA AGCCCTTCAC TCAGTGCAGA CAACCTATTG ACTGCCCACC ACACCCTGCC 
CCCCACAGCA TCACAGAAGA AGAGCTGCAC TTTGCTAAAA CCTGTCTTCA GCGTTGGAGG 
ACTGAAGTTG AGAATGACAT AAATGAACTA AAGGCCAGCA TTGACAGGCT CACTCAGACG 
CTCGAGGGCA TGTACTCAGA CAACAGTCTC TGCCAGGCCC CCTACAGACT CCATGCAGTG 
CTTGTCCATG AAGGCCAGGC ATCAGCTGGT CATTACTGGG CTTACATCTA TGACCATGCT 
AACCAGCGCT GGCTGAAGTA CAATGACATA AGTGTCACTG AGTCATCGTG GGATGAGCTG 
GAGCGGGACT CCTTTGGAGG CATGACCAAC GCAAGTGCCT ACTGCCTAAT GTACATTGAC 
GACAGGCTAC CTCAGCTCAT CACAGAAGAC ACAGATGATG AGACGGGCCA GGTGCTCCAT 
GGCATGGACT CTCTGCCACC CATCCTCAGG CGCTTTGTCC ACGAAGATAA CCGCTGGTTC 
CAGCAGGAGC TCAGCGAATG GGAGGAGCAG TTCTGCCAGA CAGCAACCCC GCAGGAAGAG 
TCCACCGCCT CTGCAGAGCC CACAAGTCCC AGTCTGGATA ACATACAGCA GTCACCTGTT 
GAGCCAGCAC CCCAGTCAGG ATCCACTACT GAAGAGGTGG ATGAAGGAGT TGCTCCAGAG 
CCACAGCCAG ACCAAGAAGC AGAGAAAGAG CCAGAACCTG ATCTCAGAAA AGAGCCAGAA 
GCGCAAACAG ATTTATCTCC CCCACCACCA GAGGGCCCTG GCTGCCAGCC AGGTGATACC 
AGCACTCCAA CAGTCACTGA CACCACGGGG GTCAGCCAGA GCTCTGATTC AGAGGAGAAG 
GAGCCGCAGA ATCAGATCCC TGGCCCTGAT GTAGAGCTTT GCGATCAGAC ACCATCTGAC 
ATCCACGTAG AGAAGGATAA CCCAGAGGTG GCCGGCCTCG TCTCTGAAAC TGAAGAAGAG 
CCCCAGGCTT CTGGAGAGGC TCCTGAACCT ATCGCAGAGC AAAGCAATCA GGAGCAGCAG 
CAGCAGCAGG AGGTTCCAGC CAGACCACGA CAGACTGAGA ATGAGGTCTC AGAAGTGGAG 
ATCCCCAATG TGGGCCGGAT CATGGTGAGG GCGGATGCTG ACGGATACAA TGAAGAGATG 
ATGCTAACTC CAGCTATGCA GGGTGTCATT CTAGCCATAG CCAAGGCCAG ACAAATTTTT 
GATAAGGAGG GTCCTGAGGC TGGCCTCATC AAGGCTTTCC ATGAAGAGTA TTCGCGCTTG 
TATGAGCTCT CTGAAGAGGA GACCACACCA CAGGAGGATG CACGTCTCCA GCACGCTCTG 
GTCTACTTCT TCCAGAACAA GGCACCCAAG CGTGTCATTG AGAGGACGCT GCTTGAGCAG 
TTTACTGACC GCAACCTCAG TTTTGATGAG AGAGCCATCA GTATCATGAG GGAAGCCCGG 
GCCAAACTTC GTCTCATTAA GCCAGAAGAC ATGGACATGG AAGAATACAT GCAGTGGCAT 
GACGATTACA GGCTGTTCAG GACGGTCTTT GTCTATCTGT TGACTGGGCT GGAGCATTAC 
CAGCACGGGA AGATGCGTGA TGCACTGAAT TACTTGGCCC ACGCCTATGA GACCAACAGC 
ACCCTGCTGC GAAACGGAGA GAAACGTGGT GTGGACAAGT CTCTCATTGC TGTTTACAGG 
AGGAAGTGTC TCACTGCACT AAATGAGAGC GCATCGCAGC TATTTTGCAG CGGTGAGGAG 
GCCAAAGTAG AAGAGGGTAT TGCCATCATG GATGAAGCCG TCATCCCCTG CCTTCACCTG 
ATGAACCGGG ACTCGACTTT ACCCCAGGAA GACCGAGATG CAATGGAGGC CATCCGCAGC 
CACTGGTGCT GCTGCCTGGG CCGAGACATG GATCAGTCAT TGCAGGTGAA GCTCGGCGAG 
TTGCTTCCCA GAGTTCTGGA CGGTTCAGCT GACACAGTAG TGCTTAAAGA CCCTCCCAAA 
GTCCACGTCA CCCAGGCCTA CGACCTGTGC AGTCGCCTGG CTGCTGTTAT GGAATCCATC 
CACAACACAT CAGTGGTCAT TGTGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004573530.2
CDS	89..3718
Translation	MFSSAMRADQSENGENSTNSQSEMLISQLREITGIQDQQILYRALNVSQGDIGHAVGLLTTQTAEVQDPGEPQESGTSGEAWEGQKGLPKDELQTAIELSLQESHKAQEEEREFHRALEASAEENAARIKRKRCEAQSEMCSPAEWIRQEDWPVGIRNVGNTCWFSAVIQSLFHLPVFRRLVLNYHLSEKILEKCKSHSDKRNIAFMQELRCLFALMVGSTRRFVDPSAAVELLRDAFRTNEAQQDVSEFSHKLLDWLEDAFQLAASGKNAEDKQHNPMVQLFYGTFVTERRHEGKMVYNIEQFGQYPLQVNGFNNLDECLEGAMVEKEIESVHSEHHVTSGRERWFKKLPPVLTFELSRFEFNTQLGRPEKIHKKLEFPQIIYMDRYLHKNIEQTHERRGEVKKLKEQLAVLQQKLECYKNYGSGPTKYPLADMLQFVLEFATTKPTSVSPAEDSRQAASSPTPPSAPLSEAIAKESSDPGESVSSESLGSSVSSCQRTPIYKPFTQCRQPIDCPPHPAPHSITEEELHFAKTCLQRWRTEVENDINELKASIDRLTQTLEGMYSDNSLCQAPYRLHAVLVHEGQASAGHYWAYIYDHANQRWLKYNDISVTESSWDELERDSFGGMTNASAYCLMYIDDRLPQLITEDTDDETGQVLHGMDSLPPILRRFVHEDNRWFQQELSEWEEQFCQTATPQEESTASAEPTSPSLDNIQQSPVEPAPQSGSTTEEVDEGVAPEPQPDQEAEKEPEPDLRKEPEAQTDLSPPPPEGPGCQPGDTSTPTVTDTTGVSQSSDSEEKEPQNQIPGPDVELCDQTPSDIHVEKDNPEVAGLVSETEEEPQASGEAPEPIAEQSNQEQQQQQEVPARPRQTENEVSEVEIPNVGRIMVRADADGYNEEMMLTPAMQGVILAIAKARQIFDKEGPEAGLIKAFHEEYSRLYELSEEETTPQEDARLQHALVYFFQNKAPKRVIERTLLEQFTDRNLSFDERAISIMREARAKLRLIKPEDMDMEEYMQWHDDYRLFRTVFVYLLTGLEHYQHGKMRDALNYLAHAYETNSTLLRNGEKRGVDKSLIAVYRRKCLTALNESASQLFCSGEEAKVEEGIAIMDEAVIPCLHLMNRDSTLPQEDRDAMEAIRSHWCCCLGRDMDQSLQVKLGELLPRVLDGSADTVVLKDPPKVHVTQAYDLCSRLAAVMESIHNTSVVIVK

Target ORF information:

RefSeq Version	XM_004573473.5
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra ubiquitin specific peptidase 28 (usp28), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004573473.5

ORF Insert Sequence:

ATGTTTTCGT CTGCAATGAG AGCCGATCAG TCTGAAAATG GAGAAAACTC AACCAACTCG 
CAGAGTGAAA TGCTGATCAG CCAGCTGAGG GAGATCACTG GCATCCAGGA CCAACAGATC 
CTCTACAGAG CCCTGAATGT CAGTCAGGGG GACATTGGAC ATGCTGTTGG ACTGCTGACA 
ACCCAGACTG CAGAGGTTCA GGACCCTGGA GAACCACAGG AGTCAGGGAC ATCTGGAGAG 
GCCTGGGAGG GCCAGAAAGG GCTTCCTAAA GATGAACTGC AGACCGCTAT AGAACTCAGC 
CTGCAGGAGT CCCACAAAGC CCAGGAAGAG GAGCGAGAAT TTCATAGGGC TCTGGAAGCC 
AGTGCAGAGG AGAATGCTGC GAGAATAAAG AGGAAGAGAT GTGAAGCCCA GAGTGAAATG 
TGTAGCCCTG CAGAGTGGAT CCGTCAGGAA GATTGGCCTG TGGGCATTCG TAATGTAGGA 
AACACCTGCT GGTTCAGTGC TGTCATCCAG TCACTCTTCC ACCTGCCTGT GTTCAGGAGA 
CTGGTTCTCA ACTACCATCT GTCTGAGAAA ATACTGGAGA AGTGTAAAAG CCATTCAGAC 
AAGAGGAACA TAGCCTTCAT GCAGGAGCTG CGCTGCCTGT TTGCCCTCAT GGTGGGCTCT 
ACCCGCAGGT TTGTGGATCC TTCTGCTGCT GTGGAGTTAT TGCGTGACGC CTTCAGGACC 
AATGAGGCCC AACAGGATGT CAGTGAGTTT TCCCACAAAC TGCTTGACTG GCTCGAGGAT 
GCATTTCAGC TGGCTGCCAG TGGAAAAAAT GCAGAAGATA AACAACATAA CCCCATGGTC 
CAGCTCTTCT ATGGTACCTT TGTGACAGAA AGAAGACACG AGGGTAAGAT GGTATATAAT 
ATTGAGCAGT TTGGCCAGTA CCCCCTGCAA GTCAATGGCT TCAACAATCT TGATGAATGC 
TTAGAAGGTG CTATGGTAGA GAAGGAGATT GAGTCGGTTC ATTCAGAGCA TCATGTCACA 
TCTGGACGTG AGAGATGGTT CAAAAAGCTA CCACCAGTCT TGACATTTGA ACTTTCGAGA 
TTTGAATTCA ACACCCAGCT TGGGCGCCCT GAGAAGATAC ATAAGAAACT GGAGTTTCCT 
CAAATCATTT ATATGGACAG ATATCTTCAC AAAAACATTG AGCAGACCCA TGAGAGGAGA 
GGAGAAGTAA AGAAACTAAA GGAGCAACTG GCAGTCCTTC AACAGAAACT TGAGTGTTAT 
AAAAACTATG GCTCTGGACC TACAAAGTAC CCTCTAGCTG ACATGCTCCA GTTTGTGCTG 
GAGTTTGCTA CCACCAAGCC GACTAGCGTT TCCCCAGCTG AAGACTCGAG ACAGGCCGCA 
TCGTCACCGA CTCCTCCAAG TGCCCCCCTC AGTGAAGCCA TTGCCAAAGA AAGCAGTGAT 
CCCGGAGAAT CAGTTTCATC AGAGAGCCTG GGTTCCAGTG TTTCCAGTTG CCAGAGGACC 
CCCATATACA AGCCCTTCAC TCAGTGCAGA CAACCTATTG ACTGCCCACC ACACCCTGCC 
CCCCACAGCA TCACAGAAGA AGAGCTGCAC TTTGCTAAAA CCTGTCTTCA GCGTTGGAGG 
ACTGAAGTTG AGAATGACAT AAATGAACTA AAGGCCAGCA TTGACAGGCT CACTCAGACG 
CTCGAGGGCA TGTACTCAGA CAACAGTCTC TGCCAGGCCC CCTACAGACT CCATGCAGTG 
CTTGTCCATG AAGGCCAGGC ATCAGCTGGT CATTACTGGG CTTACATCTA TGACCATGCT 
AACCAGCGCT GGCTGAAGTA CAATGACATA AGTGTCACTG AGTCATCGTG GGATGAGCTG 
GAGCGGGACT CCTTTGGAGG CATGACCAAC GCAAGTGCCT ACTGCCTAAT GTACATTGAC 
GACAGGCTAC CTCAGCTCAT CACAGAAGAC ACAGATGATG AGACGGGCCA GGTGCTCCAT 
GGCATGGACT CTCTGCCACC CATCCTCAGG CGCTTTGTCC ACGAAGATAA CCGCTGGTTC 
CAGCAGGAGC TCAGCGAATG GGAGGAGCAG TTCTGCCAGA CAGCAACCCC GCAGGAAGAG 
TCCACCGCCT CTGCAGAGCC CACAAGTCCC AGTCTGGATA ACATACAGCA GTCACCTGTT 
GAGCCAGCAC CCCAGTCAGG ATCCACTACT GAAGAGGTGG ATGAAGGAGT TGCTCCAGAG 
CCACAGCCAG ACCAAGAAGC AGAGAAAGAG CCAGAACCTG ATCTCAGAAA AGAGCCAGAA 
GCGCAAACAG ATTTATCTCC CCCACCACCA GAGGGCCCTG GCTGCCAGCC AGGTGATACC 
AGCACTCCAA CAGTCACTGA CACCACGGGG GTCAGCCAGA GCTCTGATTC AGAGGAGAAG 
GAGCCGCAGA ATCAGATCCC TGGCCCTGAT GTAGAGCTTT GCGATCAGAC ACCATCTGAC 
ATCCACGTAG AGAAGGATAA CCCAGAGGTG GCCGGCCTCG TCTCTGAAAC TGAAGAAGAG 
CCCCAGGCTT CTGGAGAGGC TCCTGAACCT ATCGCAGAGC AAAGCAATCA GGAGCAGCAG 
CAGCAGCAGG AGGTTCCAGC CAGACCACGA CAGACTGAGA ATGAGGTCTC AGAAGTGGAG 
ATCCCCAATG TGGGCCGGAT CATGGTGAGG GCGGATGCTG ACGGATACAA TGAAGAGATG 
ATGCTAACTC CAGCTATGCA GGGTGTCATT CTAGCCATAG CCAAGGCCAG ACAAATTTTT 
GATAAGGAGG GTCCTGAGGC TGGCCTCATC AAGGCTTTCC ATGAAGAGTA TTCGCGCTTG 
TATGAGCTCT CTGAAGAGGA GACCACACCA CAGGAGGATG CACGTCTCCA GCACGCTCTG 
GTCTACTTCT TCCAGAACAA GGCACCCAAG CGTGTCATTG AGAGGACGCT GCTTGAGCAG 
TTTACTGACC GCAACCTCAG TTTTGATGAG AGAGCCATCA GTATCATGAG GGAAGCCCGG 
GCCAAACTTC GTCTCATTAA GCCAGAAGAC ATGGACATGG AAGAATACAT GCAGTGGCAT 
GACGATTACA GGCTGTTCAG GACGGTCTTT GTCTATCTGT TGACTGGGCT GGAGCATTAC 
CAGCACGGGA AGATGCGTGA TGCACTGAAT TACTTGGCCC ACGCCTATGA GACCAACAGC 
ACCCTGCTGC GAAACGGAGA GAAACGTGGT GTGGACAAGT CTCTCATTGC TGTTTACAGG 
AGGAAGTGTC TCACTGCACT AAATGAGAGC GCATCGCAGC TATTTTGCAG CGGTGAGGAG 
GCCAAAGTAG AAGAGGGTAT TGCCATCATG GATGAAGCCG TCATCCCCTG CCTTCACCTG 
ATGAACCGGG ACTCGACTTT ACCCCAGGAA GACCGAGATG CAATGGAGGC CATCCGCAGC 
CACTGGTGCT GCTGCCTGGG CCGAGACATG GATCAGTCAT TGCAGGTGAA GCTCGGCGAG 
TTGCTTCCCA GAGTTCTGGA CGGTTCAGCT GACACAGTAG TGCTTAAAGA CCCTCCCAAA 
GTCCACGTCA CCCAGGCCTA CGACCTGTGC AGTCGCCTGG CTGCTGTTAT GGAATCCATC 
CACAACACAT CAGTGGTCAT TGTGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp28 ( XM_023153118.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_023008886.2 Maylandia zebra ubiquitin specific peptidase 28 (usp28), transcript variant X2, mRNA.

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CloneID	OMa359732
Clone ID Related Accession (Same CDS sequence)	XM_023153118.2
Accession Version	XM_023153118.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-23
Organism	Maylandia zebra(zebra mbuna)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036789.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_023153118.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTCGT CTGCAATGAG AGCCGATCAG TCTGAAAATG GAGAAAACTC AACCAACTCG 
AGTGAAATGC TGATCAGCCA GCTGAGGGAG ATCACTGGCA TCCAGGACCA ACAGATCCTC 
TACAGAGCCC TGAATGTCAG TCAGGGGGAC ATTGGACATG CTGTTGGACT GCTGACAACC 
CAGACTGCAG AGGTTCAGGA CCCTGGAGAA CCACAGGAGT CAGGGACATC TGGAGAGGCC 
TGGGAGGGCC AGAAAGGGCT TCCTAAAGAT GAACTGCAGA CCGCTATAGA ACTCAGCCTG 
CAGGAGTCCC ACAAAGCCCA GGAAGAGGAG CGAGAATTTC ATAGGGCTCT GGAAGCCAGT 
GCAGAGGAGA ATGCTGCGAG AATAAAGAGG AAGAGATGTG AAGCCCAGAG TGAAATGTGT 
AGCCCTGCAG AGTGGATCCG TCAGGAAGAT TGGCCTGTGG GCATTCGTAA TGTAGGAAAC 
ACCTGCTGGT TCAGTGCTGT CATCCAGTCA CTCTTCCACC TGCCTGTGTT CAGGAGACTG 
GTTCTCAACT ACCATCTGTC TGAGAAAATA CTGGAGAAGT GTAAAAGCCA TTCAGACAAG 
AGGAACATAG CCTTCATGCA GGAGCTGCGC TGCCTGTTTG CCCTCATGGT GGGCTCTACC 
CGCAGGTTTG TGGATCCTTC TGCTGCTGTG GAGTTATTGC GTGACGCCTT CAGGACCAAT 
GAGGCCCAAC AGGATGTCAG TGAGTTTTCC CACAAACTGC TTGACTGGCT CGAGGATGCA 
TTTCAGCTGG CTGCCAGTGG AAAAAATGCA GAAGATAAAC AACATAACCC CATGGTCCAG 
CTCTTCTATG GTACCTTTGT GACAGAAAGA AGACACGAGG GTAAGATGGT ATATAATATT 
GAGCAGTTTG GCCAGTACCC CCTGCAAGTC AATGGCTTCA ACAATCTTGA TGAATGCTTA 
GAAGGTGCTA TGGTAGAGAA GGAGATTGAG TCGGTTCATT CAGAGCATCA TGTCACATCT 
GGACGTGAGA GATGGTTCAA AAAGCTACCA CCAGTCTTGA CATTTGAACT TTCGAGATTT 
GAATTCAACA CCCAGCTTGG GCGCCCTGAG AAGATACATA AGAAACTGGA GTTTCCTCAA 
ATCATTTATA TGGACAGATA TCTTCACAAA AACATTGAGC AGACCCATGA GAGGAGAGGA 
GAAGTAAAGA AACTAAAGGA GCAACTGGCA GTCCTTCAAC AGAAACTTGA GTGTTATAAA 
AACTATGGCT CTGGACCTAC AAAGTACCCT CTAGCTGACA TGCTCCAGTT TGTGCTGGAG 
TTTGCTACCA CCAAGCCGAC TAGCGTTTCC CCAGCTGAAG ACTCGAGACA GGCCGCATCG 
TCACCGACTC CTCCAAGTGC CCCCCTCAGT GAAGCCATTG CCAAAGAAAG CAGTGATCCC 
GGAGAATCAG TTTCATCAGA GAGCCTGGGT TCCAGTGTTT CCAGTTGCCA GAGGACCCCC 
ATATACAAGC CCTTCACTCA GTGCAGACAA CCTATTGACT GCCCACCACA CCCTGCCCCC 
CACAGCATCA CAGAAGAAGA GCTGCACTTT GCTAAAACCT GTCTTCAGCG TTGGAGGACT 
GAAGTTGAGA ATGACATAAA TGAACTAAAG GCCAGCATTG ACAGGCTCAC TCAGACGCTC 
GAGGGCATGT ACTCAGACAA CAGTCTCTGC CAGGCCCCCT ACAGACTCCA TGCAGTGCTT 
GTCCATGAAG GCCAGGCATC AGCTGGTCAT TACTGGGCTT ACATCTATGA CCATGCTAAC 
CAGCGCTGGC TGAAGTACAA TGACATAAGT GTCACTGAGT CATCGTGGGA TGAGCTGGAG 
CGGGACTCCT TTGGAGGCAT GACCAACGCA AGTGCCTACT GCCTAATGTA CATTGACGAC 
AGGCTACCTC AGCTCATCAC AGAAGACACA GATGATGAGA CGGGCCAGGT GCTCCATGGC 
ATGGACTCTC TGCCACCCAT CCTCAGGCGC TTTGTCCACG AAGATAACCG CTGGTTCCAG 
CAGGAGCTCA GCGAATGGGA GGAGCAGTTC TGCCAGACAG CAACCCCGCA GGAAGAGTCC 
ACCGCCTCTG CAGAGCCCAC AAGTCCCAGT CTGGATAACA TACAGCAGTC ACCTGTTGAG 
CCAGCACCCC AGTCAGGATC CACTACTGAA GAGGTGGATG AAGGAGTTGC TCCAGAGCCA 
CAGCCAGACC AAGAAGCAGA GAAAGAGCCA GAACCTGATC TCAGAAAAGA GCCAGAAGCG 
CAAACAGATT TATCTCCCCC ACCACCAGAG GGCCCTGGCT GCCAGCCAGG TGATACCAGC 
ACTCCAACAG TCACTGACAC CACGGGGGTC AGCCAGAGCT CTGATTCAGA GGAGAAGGAG 
CCGCAGAATC AGATCCCTGG CCCTGATGTA GAGCTTTGCG ATCAGACACC ATCTGACATC 
CACGTAGAGA AGGATAACCC AGAGGTGGCC GGCCTCGTCT CTGAAACTGA AGAAGAGCCC 
CAGGCTTCTG GAGAGGCTCC TGAACCTATC GCAGAGCAAA GCAATCAGGA GCAGCAGCAG 
CAGCAGGAGG TTCCAGCCAG ACCACGACAG ACTGAGAATG AGGTCTCAGA AGTGGAGATC 
CCCAATGTGG GCCGGATCAT GGTGAGGGCG GATGCTGACG GATACAATGA AGAGATGATG 
CTAACTCCAG CTATGCAGGG TGTCATTCTA GCCATAGCCA AGGCCAGACA AATTTTTGAT 
AAGGAGGGTC CTGAGGCTGG CCTCATCAAG GCTTTCCATG AAGAGTATTC GCGCTTGTAT 
GAGCTCTCTG AAGAGGAGAC CACACCACAG GAGGATGCAC GTCTCCAGCA CGCTCTGGTC 
TACTTCTTCC AGAACAAGGC ACCCAAGCGT GTCATTGAGA GGACGCTGCT TGAGCAGTTT 
ACTGACCGCA ACCTCAGTTT TGATGAGAGA GCCATCAGTA TCATGAGGGA AGCCCGGGCC 
AAACTTCGTC TCATTAAGCC AGAAGACATG GACATGGAAG AATACATGCA GTGGCATGAC 
GATTACAGGC TGTTCAGGAC GGTCTTTGTC TATCTGTTGA CTGGGCTGGA GCATTACCAG 
CACGGGAAGA TGCGTGATGC ACTGAATTAC TTGGCCCACG CCTATGAGAC CAACAGCACC 
CTGCTGCGAA ACGGAGAGAA ACGTGGTGTG GACAAGTCTC TCATTGCTGT TTACAGGAGG 
AAGTGTCTCA CTGCACTAAA TGAGAGCGCA TCGCAGCTAT TTTGCAGCGG TGAGGAGGCC 
AAAGTAGAAG AGGGTATTGC CATCATGGAT GAAGCCGTCA TCCCCTGCCT TCACCTGATG 
AACCGGGACT CGACTTTACC CCAGGAAGAC CGAGATGCAA TGGAGGCCAT CCGCAGCCAC 
TGGTGCTGCT GCCTGGGCCG AGACATGGAT CAGTCATTGC AGGTGAAGCT CGGCGAGTTG 
CTTCCCAGAG TTCTGGACGG TTCAGCTGAC ACAGTAGTGC TTAAAGACCC TCCCAAAGTC 
CACGTCACCC AGGCCTACGA CCTGTGCAGT CGCCTGGCTG CTGTTATGGA ATCCATCCAC 
AACACATCAG TGGTCATTGT GAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023008886.2
CDS	89..3715
Translation	MFSSAMRADQSENGENSTNSSEMLISQLREITGIQDQQILYRALNVSQGDIGHAVGLLTTQTAEVQDPGEPQESGTSGEAWEGQKGLPKDELQTAIELSLQESHKAQEEEREFHRALEASAEENAARIKRKRCEAQSEMCSPAEWIRQEDWPVGIRNVGNTCWFSAVIQSLFHLPVFRRLVLNYHLSEKILEKCKSHSDKRNIAFMQELRCLFALMVGSTRRFVDPSAAVELLRDAFRTNEAQQDVSEFSHKLLDWLEDAFQLAASGKNAEDKQHNPMVQLFYGTFVTERRHEGKMVYNIEQFGQYPLQVNGFNNLDECLEGAMVEKEIESVHSEHHVTSGRERWFKKLPPVLTFELSRFEFNTQLGRPEKIHKKLEFPQIIYMDRYLHKNIEQTHERRGEVKKLKEQLAVLQQKLECYKNYGSGPTKYPLADMLQFVLEFATTKPTSVSPAEDSRQAASSPTPPSAPLSEAIAKESSDPGESVSSESLGSSVSSCQRTPIYKPFTQCRQPIDCPPHPAPHSITEEELHFAKTCLQRWRTEVENDINELKASIDRLTQTLEGMYSDNSLCQAPYRLHAVLVHEGQASAGHYWAYIYDHANQRWLKYNDISVTESSWDELERDSFGGMTNASAYCLMYIDDRLPQLITEDTDDETGQVLHGMDSLPPILRRFVHEDNRWFQQELSEWEEQFCQTATPQEESTASAEPTSPSLDNIQQSPVEPAPQSGSTTEEVDEGVAPEPQPDQEAEKEPEPDLRKEPEAQTDLSPPPPEGPGCQPGDTSTPTVTDTTGVSQSSDSEEKEPQNQIPGPDVELCDQTPSDIHVEKDNPEVAGLVSETEEEPQASGEAPEPIAEQSNQEQQQQQEVPARPRQTENEVSEVEIPNVGRIMVRADADGYNEEMMLTPAMQGVILAIAKARQIFDKEGPEAGLIKAFHEEYSRLYELSEEETTPQEDARLQHALVYFFQNKAPKRVIERTLLEQFTDRNLSFDERAISIMREARAKLRLIKPEDMDMEEYMQWHDDYRLFRTVFVYLLTGLEHYQHGKMRDALNYLAHAYETNSTLLRNGEKRGVDKSLIAVYRRKCLTALNESASQLFCSGEEAKVEEGIAIMDEAVIPCLHLMNRDSTLPQEDRDAMEAIRSHWCCCLGRDMDQSLQVKLGELLPRVLDGSADTVVLKDPPKVHVTQAYDLCSRLAAVMESIHNTSVVIVK

Target ORF information:

RefSeq Version	XM_023153118.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra ubiquitin specific peptidase 28 (usp28), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023153118.2

ORF Insert Sequence:

ATGTTTTCGT CTGCAATGAG AGCCGATCAG TCTGAAAATG GAGAAAACTC AACCAACTCG 
AGTGAAATGC TGATCAGCCA GCTGAGGGAG ATCACTGGCA TCCAGGACCA ACAGATCCTC 
TACAGAGCCC TGAATGTCAG TCAGGGGGAC ATTGGACATG CTGTTGGACT GCTGACAACC 
CAGACTGCAG AGGTTCAGGA CCCTGGAGAA CCACAGGAGT CAGGGACATC TGGAGAGGCC 
TGGGAGGGCC AGAAAGGGCT TCCTAAAGAT GAACTGCAGA CCGCTATAGA ACTCAGCCTG 
CAGGAGTCCC ACAAAGCCCA GGAAGAGGAG CGAGAATTTC ATAGGGCTCT GGAAGCCAGT 
GCAGAGGAGA ATGCTGCGAG AATAAAGAGG AAGAGATGTG AAGCCCAGAG TGAAATGTGT 
AGCCCTGCAG AGTGGATCCG TCAGGAAGAT TGGCCTGTGG GCATTCGTAA TGTAGGAAAC 
ACCTGCTGGT TCAGTGCTGT CATCCAGTCA CTCTTCCACC TGCCTGTGTT CAGGAGACTG 
GTTCTCAACT ACCATCTGTC TGAGAAAATA CTGGAGAAGT GTAAAAGCCA TTCAGACAAG 
AGGAACATAG CCTTCATGCA GGAGCTGCGC TGCCTGTTTG CCCTCATGGT GGGCTCTACC 
CGCAGGTTTG TGGATCCTTC TGCTGCTGTG GAGTTATTGC GTGACGCCTT CAGGACCAAT 
GAGGCCCAAC AGGATGTCAG TGAGTTTTCC CACAAACTGC TTGACTGGCT CGAGGATGCA 
TTTCAGCTGG CTGCCAGTGG AAAAAATGCA GAAGATAAAC AACATAACCC CATGGTCCAG 
CTCTTCTATG GTACCTTTGT GACAGAAAGA AGACACGAGG GTAAGATGGT ATATAATATT 
GAGCAGTTTG GCCAGTACCC CCTGCAAGTC AATGGCTTCA ACAATCTTGA TGAATGCTTA 
GAAGGTGCTA TGGTAGAGAA GGAGATTGAG TCGGTTCATT CAGAGCATCA TGTCACATCT 
GGACGTGAGA GATGGTTCAA AAAGCTACCA CCAGTCTTGA CATTTGAACT TTCGAGATTT 
GAATTCAACA CCCAGCTTGG GCGCCCTGAG AAGATACATA AGAAACTGGA GTTTCCTCAA 
ATCATTTATA TGGACAGATA TCTTCACAAA AACATTGAGC AGACCCATGA GAGGAGAGGA 
GAAGTAAAGA AACTAAAGGA GCAACTGGCA GTCCTTCAAC AGAAACTTGA GTGTTATAAA 
AACTATGGCT CTGGACCTAC AAAGTACCCT CTAGCTGACA TGCTCCAGTT TGTGCTGGAG 
TTTGCTACCA CCAAGCCGAC TAGCGTTTCC CCAGCTGAAG ACTCGAGACA GGCCGCATCG 
TCACCGACTC CTCCAAGTGC CCCCCTCAGT GAAGCCATTG CCAAAGAAAG CAGTGATCCC 
GGAGAATCAG TTTCATCAGA GAGCCTGGGT TCCAGTGTTT CCAGTTGCCA GAGGACCCCC 
ATATACAAGC CCTTCACTCA GTGCAGACAA CCTATTGACT GCCCACCACA CCCTGCCCCC 
CACAGCATCA CAGAAGAAGA GCTGCACTTT GCTAAAACCT GTCTTCAGCG TTGGAGGACT 
GAAGTTGAGA ATGACATAAA TGAACTAAAG GCCAGCATTG ACAGGCTCAC TCAGACGCTC 
GAGGGCATGT ACTCAGACAA CAGTCTCTGC CAGGCCCCCT ACAGACTCCA TGCAGTGCTT 
GTCCATGAAG GCCAGGCATC AGCTGGTCAT TACTGGGCTT ACATCTATGA CCATGCTAAC 
CAGCGCTGGC TGAAGTACAA TGACATAAGT GTCACTGAGT CATCGTGGGA TGAGCTGGAG 
CGGGACTCCT TTGGAGGCAT GACCAACGCA AGTGCCTACT GCCTAATGTA CATTGACGAC 
AGGCTACCTC AGCTCATCAC AGAAGACACA GATGATGAGA CGGGCCAGGT GCTCCATGGC 
ATGGACTCTC TGCCACCCAT CCTCAGGCGC TTTGTCCACG AAGATAACCG CTGGTTCCAG 
CAGGAGCTCA GCGAATGGGA GGAGCAGTTC TGCCAGACAG CAACCCCGCA GGAAGAGTCC 
ACCGCCTCTG CAGAGCCCAC AAGTCCCAGT CTGGATAACA TACAGCAGTC ACCTGTTGAG 
CCAGCACCCC AGTCAGGATC CACTACTGAA GAGGTGGATG AAGGAGTTGC TCCAGAGCCA 
CAGCCAGACC AAGAAGCAGA GAAAGAGCCA GAACCTGATC TCAGAAAAGA GCCAGAAGCG 
CAAACAGATT TATCTCCCCC ACCACCAGAG GGCCCTGGCT GCCAGCCAGG TGATACCAGC 
ACTCCAACAG TCACTGACAC CACGGGGGTC AGCCAGAGCT CTGATTCAGA GGAGAAGGAG 
CCGCAGAATC AGATCCCTGG CCCTGATGTA GAGCTTTGCG ATCAGACACC ATCTGACATC 
CACGTAGAGA AGGATAACCC AGAGGTGGCC GGCCTCGTCT CTGAAACTGA AGAAGAGCCC 
CAGGCTTCTG GAGAGGCTCC TGAACCTATC GCAGAGCAAA GCAATCAGGA GCAGCAGCAG 
CAGCAGGAGG TTCCAGCCAG ACCACGACAG ACTGAGAATG AGGTCTCAGA AGTGGAGATC 
CCCAATGTGG GCCGGATCAT GGTGAGGGCG GATGCTGACG GATACAATGA AGAGATGATG 
CTAACTCCAG CTATGCAGGG TGTCATTCTA GCCATAGCCA AGGCCAGACA AATTTTTGAT 
AAGGAGGGTC CTGAGGCTGG CCTCATCAAG GCTTTCCATG AAGAGTATTC GCGCTTGTAT 
GAGCTCTCTG AAGAGGAGAC CACACCACAG GAGGATGCAC GTCTCCAGCA CGCTCTGGTC 
TACTTCTTCC AGAACAAGGC ACCCAAGCGT GTCATTGAGA GGACGCTGCT TGAGCAGTTT 
ACTGACCGCA ACCTCAGTTT TGATGAGAGA GCCATCAGTA TCATGAGGGA AGCCCGGGCC 
AAACTTCGTC TCATTAAGCC AGAAGACATG GACATGGAAG AATACATGCA GTGGCATGAC 
GATTACAGGC TGTTCAGGAC GGTCTTTGTC TATCTGTTGA CTGGGCTGGA GCATTACCAG 
CACGGGAAGA TGCGTGATGC ACTGAATTAC TTGGCCCACG CCTATGAGAC CAACAGCACC 
CTGCTGCGAA ACGGAGAGAA ACGTGGTGTG GACAAGTCTC TCATTGCTGT TTACAGGAGG 
AAGTGTCTCA CTGCACTAAA TGAGAGCGCA TCGCAGCTAT TTTGCAGCGG TGAGGAGGCC 
AAAGTAGAAG AGGGTATTGC CATCATGGAT GAAGCCGTCA TCCCCTGCCT TCACCTGATG 
AACCGGGACT CGACTTTACC CCAGGAAGAC CGAGATGCAA TGGAGGCCAT CCGCAGCCAC 
TGGTGCTGCT GCCTGGGCCG AGACATGGAT CAGTCATTGC AGGTGAAGCT CGGCGAGTTG 
CTTCCCAGAG TTCTGGACGG TTCAGCTGAC ACAGTAGTGC TTAAAGACCC TCCCAAAGTC 
CACGTCACCC AGGCCTACGA CCTGTGCAGT CGCCTGGCTG CTGTTATGGA ATCCATCCAC 
AACACATCAG TGGTCATTGT GAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp28 ( XM_023153119.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_023008887.2 Maylandia zebra ubiquitin specific peptidase 28 (usp28), transcript variant X3, mRNA.

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CloneID	OMa359733
Clone ID Related Accession (Same CDS sequence)	XM_023153119.2
Accession Version	XM_023153119.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-23
Organism	Maylandia zebra(zebra mbuna)
Product	ubiquitin carboxyl-terminal hydrolase 28 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036789.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_023153119.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTCGT CTGCAATGAG AGCCGATCAG TCTGAAAATG GAGAAAACTC AACCAACTCG 
CAGAGTGAAA TGCTGATCAG CCAGCTGAGG GAGATCACTG GCATCCAGGA CCAACAGATC 
CTCTACAGAG CCCTGAATGT CAGTCAGGGG GACATTGGAC ATGCTGTTGG ACTGCTGACA 
ACCCAGACTG CAGAGGTTCA GGACCCTGGA GAACCACAGG AGTCAGGGAC ATCTGGAGAG 
GCCTGGGAGG GCCAGAAAGG GCTTCCTAAA GATGAACTGC AGACCGCTAT AGAACTCAGC 
CTGCAGGAGT CCCACAAAGC CCAGGAAGAG GAGCGAGAAT TTCATAGGGC TCTGGAAGCC 
AGTGCAGAGG AGAATGCTGC GAGAATAAAG AGGAAGAGAT GTGAAGCCCA GAGTGAAATG 
TGTAGCCCTG CAGAGTGGAT CCGTCAGGAA GATTGGCCTG TGGGCATTCG TAATGTAGGA 
AACACCTGCT GGTTCAGTGC TGTCATCCAG TCACTCTTCC ACCTGCCTGT GTTCAGGAGA 
CTGGTTCTCA ACTACCATCT GTCTGAGAAA ATACTGGAGA AGTGTAAAAG CCATTCAGAC 
AAGAGGAACA TAGCCTTCAT GCAGGAGCTG CGCTGCCTGT TTGCCCTCAT GGTGGGCTCT 
ACCCGCAGGT TTGTGGATCC TTCTGCTGCT GTGGAGTTAT TGCGTGACGC CTTCAGGACC 
AATGAGGCCC AACAGGATGT CAGTGAGTTT TCCCACAAAC TGCTTGACTG GCTCGAGGAT 
GCATTTCAGC TGGCTGCCAG TGGAAAAAAT GCAGAAGATA AACAACATAA CCCCATGGTC 
CAGCTCTTCT ATGGTACCTT TGTGACAGAA AGAAGACACG AGGGTAAGAT GGTATATAAT 
ATTGAGCAGT TTGGCCAGTA CCCCCTGCAA GTCAATGGCT TCAACAATCT TGATGAATGC 
TTAGAAGGTG CTATGGTAGA GAAGGAGATT GAGTCGGTTC ATTCAGAGCA TCATGTCACA 
TCTGGACGTG AGAGATGGTT CAAAAAGCTA CCACCAGTCT TGACATTTGA ACTTTCGAGA 
TTTGAATTCA ACACCCAGCT TGGGCGCCCT GAGAAGATAC ATAAGAAACT GGAGTTTCCT 
CAAATCATTT ATATGGACAG ATATCTTCAC AAAAACATTG AGCAGACCCA TGAGAGGAGA 
GGAGAAGTAA AGAAACTAAA GGAGCAACTG GCAGTCCTTC AACAGAAACT TGAGTGTTAT 
AAAAACTATG GCTCTGGACC TACAAAGTAC CCTCTAGCTG ACATGCTCCA GTTTGTGCTG 
GAGTTTGCTA CCACCAAGCC GACTAGCGTT TCCCCAGCTG AAGACTCGAG ACAGGCCGCA 
TCGTCACCGA CTCCTCCAAG TGCCCCCCTC AGTGAAGCCA TTGCCAAAGA AAGCAGTGAT 
CCCGGAGAAT CAGTTTCATC AGAGAGCCTG GGTTCCAGTG TTTCCAGTTG CCAGAGGACC 
CCCATATACA AGCCCTTCAC TCAGTGCAGA CAACCTATTG ACTGCCCACC ACACCCTGCC 
CCCCACAGCA TCACAGAAGA AGAGCTGCAC TTTGCTAAAA CCTGTCTTCA GCGTTGGAGG 
ACTGAAGTTG AGAATGACAT AAATGAACTA AAGGCCAGCA TTGACAGGCT CACTCAGACG 
CTCGAGGGCA TGTACTCAGA CAACAGTCTC TGCCAGGCCC CCTACAGACT CCATGCAGTG 
CTTGTCCATG AAGGCCAGGC ATCAGCTGGT CATTACTGGG CTTACATCTA TGACCATGCT 
AACCAGCGCT GGCTGAAGTA CAATGACATA AGTGTCACTG AGTCATCGTG GGATGAGCTG 
GAGCGGGACT CCTTTGGAGG CATGACCAAC GCAAGTGCCT ACTGCCTAAT GTACATTGAC 
GACAGGCTAC CTCAGCTCAT CACAGACACA GATGATGAGA CGGGCCAGGT GCTCCATGGC 
ATGGACTCTC TGCCACCCAT CCTCAGGCGC TTTGTCCACG AAGATAACCG CTGGTTCCAG 
CAGGAGCTCA GCGAATGGGA GGAGCAGTTC TGCCAGACAG CAACCCCGCA GGAAGAGTCC 
ACCGCCTCTG CAGAGCCCAC AAGTCCCAGT CTGGATAACA TACAGCAGTC ACCTGTTGAG 
CCAGCACCCC AGTCAGGATC CACTACTGAA GAGGTGGATG AAGGAGTTGC TCCAGAGCCA 
CAGCCAGACC AAGAAGCAGA GAAAGAGCCA GAACCTGATC TCAGAAAAGA GCCAGAAGCG 
CAAACAGATT TATCTCCCCC ACCACCAGAG GGCCCTGGCT GCCAGCCAGG TGATACCAGC 
ACTCCAACAG TCACTGACAC CACGGGGGTC AGCCAGAGCT CTGATTCAGA GGAGAAGGAG 
CCGCAGAATC AGATCCCTGG CCCTGATGTA GAGCTTTGCG ATCAGACACC ATCTGACATC 
CACGTAGAGA AGGATAACCC AGAGGTGGCC GGCCTCGTCT CTGAAACTGA AGAAGAGCCC 
CAGGCTTCTG GAGAGGCTCC TGAACCTATC GCAGAGCAAA GCAATCAGGA GCAGCAGCAG 
CAGCAGGAGG TTCCAGCCAG ACCACGACAG ACTGAGAATG AGGTCTCAGA AGTGGAGATC 
CCCAATGTGG GCCGGATCAT GGTGAGGGCG GATGCTGACG GATACAATGA AGAGATGATG 
CTAACTCCAG CTATGCAGGG TGTCATTCTA GCCATAGCCA AGGCCAGACA AATTTTTGAT 
AAGGAGGGTC CTGAGGCTGG CCTCATCAAG GCTTTCCATG AAGAGTATTC GCGCTTGTAT 
GAGCTCTCTG AAGAGGAGAC CACACCACAG GAGGATGCAC GTCTCCAGCA CGCTCTGGTC 
TACTTCTTCC AGAACAAGGC ACCCAAGCGT GTCATTGAGA GGACGCTGCT TGAGCAGTTT 
ACTGACCGCA ACCTCAGTTT TGATGAGAGA GCCATCAGTA TCATGAGGGA AGCCCGGGCC 
AAACTTCGTC TCATTAAGCC AGAAGACATG GACATGGAAG AATACATGCA GTGGCATGAC 
GATTACAGGC TGTTCAGGAC GGTCTTTGTC TATCTGTTGA CTGGGCTGGA GCATTACCAG 
CACGGGAAGA TGCGTGATGC ACTGAATTAC TTGGCCCACG CCTATGAGAC CAACAGCACC 
CTGCTGCGAA ACGGAGAGAA ACGTGGTGTG GACAAGTCTC TCATTGCTGT TTACAGGAGG 
AAGTGTCTCA CTGCACTAAA TGAGAGCGCA TCGCAGCTAT TTTGCAGCGG TGAGGAGGCC 
AAAGTAGAAG AGGGTATTGC CATCATGGAT GAAGCCGTCA TCCCCTGCCT TCACCTGATG 
AACCGGGACT CGACTTTACC CCAGGAAGAC CGAGATGCAA TGGAGGCCAT CCGCAGCCAC 
TGGTGCTGCT GCCTGGGCCG AGACATGGAT CAGTCATTGC AGGTGAAGCT CGGCGAGTTG 
CTTCCCAGAG TTCTGGACGG TTCAGCTGAC ACAGTAGTGC TTAAAGACCC TCCCAAAGTC 
CACGTCACCC AGGCCTACGA CCTGTGCAGT CGCCTGGCTG CTGTTATGGA ATCCATCCAC 
AACACATCAG TGGTCATTGT GAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023008887.2
CDS	89..3715
Translation	MFSSAMRADQSENGENSTNSQSEMLISQLREITGIQDQQILYRALNVSQGDIGHAVGLLTTQTAEVQDPGEPQESGTSGEAWEGQKGLPKDELQTAIELSLQESHKAQEEEREFHRALEASAEENAARIKRKRCEAQSEMCSPAEWIRQEDWPVGIRNVGNTCWFSAVIQSLFHLPVFRRLVLNYHLSEKILEKCKSHSDKRNIAFMQELRCLFALMVGSTRRFVDPSAAVELLRDAFRTNEAQQDVSEFSHKLLDWLEDAFQLAASGKNAEDKQHNPMVQLFYGTFVTERRHEGKMVYNIEQFGQYPLQVNGFNNLDECLEGAMVEKEIESVHSEHHVTSGRERWFKKLPPVLTFELSRFEFNTQLGRPEKIHKKLEFPQIIYMDRYLHKNIEQTHERRGEVKKLKEQLAVLQQKLECYKNYGSGPTKYPLADMLQFVLEFATTKPTSVSPAEDSRQAASSPTPPSAPLSEAIAKESSDPGESVSSESLGSSVSSCQRTPIYKPFTQCRQPIDCPPHPAPHSITEEELHFAKTCLQRWRTEVENDINELKASIDRLTQTLEGMYSDNSLCQAPYRLHAVLVHEGQASAGHYWAYIYDHANQRWLKYNDISVTESSWDELERDSFGGMTNASAYCLMYIDDRLPQLITDTDDETGQVLHGMDSLPPILRRFVHEDNRWFQQELSEWEEQFCQTATPQEESTASAEPTSPSLDNIQQSPVEPAPQSGSTTEEVDEGVAPEPQPDQEAEKEPEPDLRKEPEAQTDLSPPPPEGPGCQPGDTSTPTVTDTTGVSQSSDSEEKEPQNQIPGPDVELCDQTPSDIHVEKDNPEVAGLVSETEEEPQASGEAPEPIAEQSNQEQQQQQEVPARPRQTENEVSEVEIPNVGRIMVRADADGYNEEMMLTPAMQGVILAIAKARQIFDKEGPEAGLIKAFHEEYSRLYELSEEETTPQEDARLQHALVYFFQNKAPKRVIERTLLEQFTDRNLSFDERAISIMREARAKLRLIKPEDMDMEEYMQWHDDYRLFRTVFVYLLTGLEHYQHGKMRDALNYLAHAYETNSTLLRNGEKRGVDKSLIAVYRRKCLTALNESASQLFCSGEEAKVEEGIAIMDEAVIPCLHLMNRDSTLPQEDRDAMEAIRSHWCCCLGRDMDQSLQVKLGELLPRVLDGSADTVVLKDPPKVHVTQAYDLCSRLAAVMESIHNTSVVIVK

Target ORF information:

RefSeq Version	XM_023153119.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra ubiquitin specific peptidase 28 (usp28), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023153119.2

ORF Insert Sequence:

ATGTTTTCGT CTGCAATGAG AGCCGATCAG TCTGAAAATG GAGAAAACTC AACCAACTCG 
CAGAGTGAAA TGCTGATCAG CCAGCTGAGG GAGATCACTG GCATCCAGGA CCAACAGATC 
CTCTACAGAG CCCTGAATGT CAGTCAGGGG GACATTGGAC ATGCTGTTGG ACTGCTGACA 
ACCCAGACTG CAGAGGTTCA GGACCCTGGA GAACCACAGG AGTCAGGGAC ATCTGGAGAG 
GCCTGGGAGG GCCAGAAAGG GCTTCCTAAA GATGAACTGC AGACCGCTAT AGAACTCAGC 
CTGCAGGAGT CCCACAAAGC CCAGGAAGAG GAGCGAGAAT TTCATAGGGC TCTGGAAGCC 
AGTGCAGAGG AGAATGCTGC GAGAATAAAG AGGAAGAGAT GTGAAGCCCA GAGTGAAATG 
TGTAGCCCTG CAGAGTGGAT CCGTCAGGAA GATTGGCCTG TGGGCATTCG TAATGTAGGA 
AACACCTGCT GGTTCAGTGC TGTCATCCAG TCACTCTTCC ACCTGCCTGT GTTCAGGAGA 
CTGGTTCTCA ACTACCATCT GTCTGAGAAA ATACTGGAGA AGTGTAAAAG CCATTCAGAC 
AAGAGGAACA TAGCCTTCAT GCAGGAGCTG CGCTGCCTGT TTGCCCTCAT GGTGGGCTCT 
ACCCGCAGGT TTGTGGATCC TTCTGCTGCT GTGGAGTTAT TGCGTGACGC CTTCAGGACC 
AATGAGGCCC AACAGGATGT CAGTGAGTTT TCCCACAAAC TGCTTGACTG GCTCGAGGAT 
GCATTTCAGC TGGCTGCCAG TGGAAAAAAT GCAGAAGATA AACAACATAA CCCCATGGTC 
CAGCTCTTCT ATGGTACCTT TGTGACAGAA AGAAGACACG AGGGTAAGAT GGTATATAAT 
ATTGAGCAGT TTGGCCAGTA CCCCCTGCAA GTCAATGGCT TCAACAATCT TGATGAATGC 
TTAGAAGGTG CTATGGTAGA GAAGGAGATT GAGTCGGTTC ATTCAGAGCA TCATGTCACA 
TCTGGACGTG AGAGATGGTT CAAAAAGCTA CCACCAGTCT TGACATTTGA ACTTTCGAGA 
TTTGAATTCA ACACCCAGCT TGGGCGCCCT GAGAAGATAC ATAAGAAACT GGAGTTTCCT 
CAAATCATTT ATATGGACAG ATATCTTCAC AAAAACATTG AGCAGACCCA TGAGAGGAGA 
GGAGAAGTAA AGAAACTAAA GGAGCAACTG GCAGTCCTTC AACAGAAACT TGAGTGTTAT 
AAAAACTATG GCTCTGGACC TACAAAGTAC CCTCTAGCTG ACATGCTCCA GTTTGTGCTG 
GAGTTTGCTA CCACCAAGCC GACTAGCGTT TCCCCAGCTG AAGACTCGAG ACAGGCCGCA 
TCGTCACCGA CTCCTCCAAG TGCCCCCCTC AGTGAAGCCA TTGCCAAAGA AAGCAGTGAT 
CCCGGAGAAT CAGTTTCATC AGAGAGCCTG GGTTCCAGTG TTTCCAGTTG CCAGAGGACC 
CCCATATACA AGCCCTTCAC TCAGTGCAGA CAACCTATTG ACTGCCCACC ACACCCTGCC 
CCCCACAGCA TCACAGAAGA AGAGCTGCAC TTTGCTAAAA CCTGTCTTCA GCGTTGGAGG 
ACTGAAGTTG AGAATGACAT AAATGAACTA AAGGCCAGCA TTGACAGGCT CACTCAGACG 
CTCGAGGGCA TGTACTCAGA CAACAGTCTC TGCCAGGCCC CCTACAGACT CCATGCAGTG 
CTTGTCCATG AAGGCCAGGC ATCAGCTGGT CATTACTGGG CTTACATCTA TGACCATGCT 
AACCAGCGCT GGCTGAAGTA CAATGACATA AGTGTCACTG AGTCATCGTG GGATGAGCTG 
GAGCGGGACT CCTTTGGAGG CATGACCAAC GCAAGTGCCT ACTGCCTAAT GTACATTGAC 
GACAGGCTAC CTCAGCTCAT CACAGACACA GATGATGAGA CGGGCCAGGT GCTCCATGGC 
ATGGACTCTC TGCCACCCAT CCTCAGGCGC TTTGTCCACG AAGATAACCG CTGGTTCCAG 
CAGGAGCTCA GCGAATGGGA GGAGCAGTTC TGCCAGACAG CAACCCCGCA GGAAGAGTCC 
ACCGCCTCTG CAGAGCCCAC AAGTCCCAGT CTGGATAACA TACAGCAGTC ACCTGTTGAG 
CCAGCACCCC AGTCAGGATC CACTACTGAA GAGGTGGATG AAGGAGTTGC TCCAGAGCCA 
CAGCCAGACC AAGAAGCAGA GAAAGAGCCA GAACCTGATC TCAGAAAAGA GCCAGAAGCG 
CAAACAGATT TATCTCCCCC ACCACCAGAG GGCCCTGGCT GCCAGCCAGG TGATACCAGC 
ACTCCAACAG TCACTGACAC CACGGGGGTC AGCCAGAGCT CTGATTCAGA GGAGAAGGAG 
CCGCAGAATC AGATCCCTGG CCCTGATGTA GAGCTTTGCG ATCAGACACC ATCTGACATC 
CACGTAGAGA AGGATAACCC AGAGGTGGCC GGCCTCGTCT CTGAAACTGA AGAAGAGCCC 
CAGGCTTCTG GAGAGGCTCC TGAACCTATC GCAGAGCAAA GCAATCAGGA GCAGCAGCAG 
CAGCAGGAGG TTCCAGCCAG ACCACGACAG ACTGAGAATG AGGTCTCAGA AGTGGAGATC 
CCCAATGTGG GCCGGATCAT GGTGAGGGCG GATGCTGACG GATACAATGA AGAGATGATG 
CTAACTCCAG CTATGCAGGG TGTCATTCTA GCCATAGCCA AGGCCAGACA AATTTTTGAT 
AAGGAGGGTC CTGAGGCTGG CCTCATCAAG GCTTTCCATG AAGAGTATTC GCGCTTGTAT 
GAGCTCTCTG AAGAGGAGAC CACACCACAG GAGGATGCAC GTCTCCAGCA CGCTCTGGTC 
TACTTCTTCC AGAACAAGGC ACCCAAGCGT GTCATTGAGA GGACGCTGCT TGAGCAGTTT 
ACTGACCGCA ACCTCAGTTT TGATGAGAGA GCCATCAGTA TCATGAGGGA AGCCCGGGCC 
AAACTTCGTC TCATTAAGCC AGAAGACATG GACATGGAAG AATACATGCA GTGGCATGAC 
GATTACAGGC TGTTCAGGAC GGTCTTTGTC TATCTGTTGA CTGGGCTGGA GCATTACCAG 
CACGGGAAGA TGCGTGATGC ACTGAATTAC TTGGCCCACG CCTATGAGAC CAACAGCACC 
CTGCTGCGAA ACGGAGAGAA ACGTGGTGTG GACAAGTCTC TCATTGCTGT TTACAGGAGG 
AAGTGTCTCA CTGCACTAAA TGAGAGCGCA TCGCAGCTAT TTTGCAGCGG TGAGGAGGCC 
AAAGTAGAAG AGGGTATTGC CATCATGGAT GAAGCCGTCA TCCCCTGCCT TCACCTGATG 
AACCGGGACT CGACTTTACC CCAGGAAGAC CGAGATGCAA TGGAGGCCAT CCGCAGCCAC 
TGGTGCTGCT GCCTGGGCCG AGACATGGAT CAGTCATTGC AGGTGAAGCT CGGCGAGTTG 
CTTCCCAGAG TTCTGGACGG TTCAGCTGAC ACAGTAGTGC TTAAAGACCC TCCCAAAGTC 
CACGTCACCC AGGCCTACGA CCTGTGCAGT CGCCTGGCTG CTGTTATGGA ATCCATCCAC 
AACACATCAG TGGTCATTGT GAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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usp28 cDNA ORF clone, Maylandia zebra(zebra mbuna)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム