NAE1 cDNA ORF clone, Manis javanica(Malayan pangolin)

Gene
Clones
gRNAs

Summary

Gene Symbol	NAE1
Entrez Gene ID	108390333
Full Name	NEDD8 activating enzyme E1 subunit 1
Gene Type	protein-coding
Organism	Manis javanica(Malayan pangolin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017649170.1	XP_017504659.1	NEDD8-activating enzyme E1 regulatory subunit

Pathways from BioSystems
Gene Ontology

The following NAE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NAE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMa169449	XM_017649170.1 Latest version!	Manis javanica NEDD8 activating enzyme E1 subunit 1 (NAE1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NAE1 ( XM_017649170.1 ) cDNA ORF clone, Manis javanica(Malayan pangolin) -> XP_017504659.1 Manis javanica NEDD8 activating enzyme E1 subunit 1 (NAE1), mRNA.

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CloneID	OMa169449
Clone ID Related Accession (Same CDS sequence)	XM_017649170.1
Accession Version	XM_017649170.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-28
Organism	Manis javanica(Malayan pangolin)
Product	NEDD8-activating enzyme E1 regulatory subunit
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016528794.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Manis javanica Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCAGC TGGGCAGGCT GCTCAAGGAG CAGAAATACG ACCGGCAGCT GAGGTTGTGG 
GGTGATCATG GGCAAGAGGC TTTAGAATCT GCTCATGTTT GCCTAATAAA CGCAACAGCT 
ACAGGAACTG AAATTCTTAA GAACTTAGTA TTACCAGGTA TTGGTTCATT TACAATTATT 
GATGGAAATC AAGTCAGTGG AGAAGATGCT GGAAATAATT TTTTCCTTCA AAGAAGCAGT 
ATTGGCAAGA ATCGTGCTCA AGCTGCCATG GAATTCTTAA AAGAATTAAA TAGTGATGTC 
TCTGGAAGTT TTGTGGAAGA GAGTCCAGAA AACCTTCTAG ACAATGACCC TTCATTTTTC 
TGTAGGTTTA CCATTGTGGT TGCAACTCAG CTTCCTGAAA GTATGTTACT ACGTTTAGCA 
GATGTCCTCT GGAATTCCCA AATCCCTCTT TTGATCTGTA GAACATACGG ACTAGTTGGT 
TATATGAGGA TCATTATAAA AGAACATCCA GTAATAGAAT CTCATCCAGA TAATGCATTA 
GAGGATCTAC GACTGGATAA ACCATTTCCT GAACTGAGAG AACATTTTCA GTCTTATGAT 
TTGGATCATA TGGAAAAAAA GGACCATAGC CACACGCCAT GGATCGTGAT TGTAGCTAAG 
TATTTAGCAC AGTGGCATAG TGAAACAAAT GGACGAATAC CTAAAACGTA TAAAGAAAAA 
GAAGACTTCA GAGATTTGAT TAGACAAGGA GTTTTAAAGA ATGAAAATGG GACTCCAGAA 
GATGAAGAGA ATTTTGAAGA AGCTATCAAA AATGTGAACA CGGCACTAAA TACAACGCAG 
ATCCCAAGCA GTATTGAAGA TATATTTAAT GATGATTGCT GCATAAATAT CACCAAACAG 
ACTCCATCAT TTTGGATTTT AGCTCGTGCC TTAAAGGAAT TTGTGGCCAA AGAAGGTCAA 
GGAAATTTAC CTGTTCGAGG CACAATTCCT GATATGATTG CGGATTCAAG CAAATACATA 
AAACTACAAA ATGTTTACCG TGAGAAAGCA AAGAAAGATG CTGCTGCTGT GGGTAATTAT 
GTTGCCCAAT TGCTGCAGTC TATAGGCCAG GCACCAGAGT CCATTTCAGA GAAAGAATTA 
AAATTACTCT GCAGCAATTC TGCATTTCTT CAAGTGGTAA GATGTCGATC CTTAGCTGAA 
GAATATGGCT TAGATACAAT TAACAAGGAT GAAATTATTT CCAGCATGGA CAATCCAGAT 
AATGAGATAG TATTATACTT GATGTTACGG GCTGTTGATA GATTTTATAA ACAACACGGT 
AGATATCCAG GAGTATCTAA CTATCAAGTT GAAGAAGATG TAGGAAAACT GAAGTCTTGT 
CTCACTGGCT TCCTTCAGGA ACATGGATTA TCTGTAATGG TGAAAGATGA TTATGTCCAT 
GAATTTTGCC GATATGGAGC TGCTGAGCCA CACACCATTG CTGCTTTCTT AGGGGGAGCT 
GCTGCTCAAG AGGTTATCAA AGTAATTACA AAACAATTTG TAATTTTTAA TAATACATAC 
ATTTATAGTG GCATGTCACA AACTTCAGCA ACTTTCCAGT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017504659.1
CDS	60..1664
Translation	MAQLGRLLKEQKYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGSFTIIDGNQVSGEDAGNNFFLQRSSIGKNRAQAAMEFLKELNSDVSGSFVEESPENLLDNDPSFFCRFTIVVATQLPESMLLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDNALEDLRLDKPFPELREHFQSYDLDHMEKKDHSHTPWIVIVAKYLAQWHSETNGRIPKTYKEKEDFRDLIRQGVLKNENGTPEDEENFEEAIKNVNTALNTTQIPSSIEDIFNDDCCINITKQTPSFWILARALKEFVAKEGQGNLPVRGTIPDMIADSSKYIKLQNVYREKAKKDAAAVGNYVAQLLQSIGQAPESISEKELKLLCSNSAFLQVVRCRSLAEEYGLDTINKDEIISSMDNPDNEIVLYLMLRAVDRFYKQHGRYPGVSNYQVEEDVGKLKSCLTGFLQEHGLSVMVKDDYVHEFCRYGAAEPHTIAAFLGGAAAQEVIKVITKQFVIFNNTYIYSGMSQTSATFQL

Target ORF information:

RefSeq Version	XM_017649170.1
Organism	Manis javanica(Malayan pangolin)
Definition	Manis javanica NEDD8 activating enzyme E1 subunit 1 (NAE1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017649170.1

ORF Insert Sequence:

ATGGCGCAGC TGGGCAGGCT GCTCAAGGAG CAGAAATACG ACCGGCAGCT GAGGTTGTGG 
GGTGATCATG GGCAAGAGGC TTTAGAATCT GCTCATGTTT GCCTAATAAA CGCAACAGCT 
ACAGGAACTG AAATTCTTAA GAACTTAGTA TTACCAGGTA TTGGTTCATT TACAATTATT 
GATGGAAATC AAGTCAGTGG AGAAGATGCT GGAAATAATT TTTTCCTTCA AAGAAGCAGT 
ATTGGCAAGA ATCGTGCTCA AGCTGCCATG GAATTCTTAA AAGAATTAAA TAGTGATGTC 
TCTGGAAGTT TTGTGGAAGA GAGTCCAGAA AACCTTCTAG ACAATGACCC TTCATTTTTC 
TGTAGGTTTA CCATTGTGGT TGCAACTCAG CTTCCTGAAA GTATGTTACT ACGTTTAGCA 
GATGTCCTCT GGAATTCCCA AATCCCTCTT TTGATCTGTA GAACATACGG ACTAGTTGGT 
TATATGAGGA TCATTATAAA AGAACATCCA GTAATAGAAT CTCATCCAGA TAATGCATTA 
GAGGATCTAC GACTGGATAA ACCATTTCCT GAACTGAGAG AACATTTTCA GTCTTATGAT 
TTGGATCATA TGGAAAAAAA GGACCATAGC CACACGCCAT GGATCGTGAT TGTAGCTAAG 
TATTTAGCAC AGTGGCATAG TGAAACAAAT GGACGAATAC CTAAAACGTA TAAAGAAAAA 
GAAGACTTCA GAGATTTGAT TAGACAAGGA GTTTTAAAGA ATGAAAATGG GACTCCAGAA 
GATGAAGAGA ATTTTGAAGA AGCTATCAAA AATGTGAACA CGGCACTAAA TACAACGCAG 
ATCCCAAGCA GTATTGAAGA TATATTTAAT GATGATTGCT GCATAAATAT CACCAAACAG 
ACTCCATCAT TTTGGATTTT AGCTCGTGCC TTAAAGGAAT TTGTGGCCAA AGAAGGTCAA 
GGAAATTTAC CTGTTCGAGG CACAATTCCT GATATGATTG CGGATTCAAG CAAATACATA 
AAACTACAAA ATGTTTACCG TGAGAAAGCA AAGAAAGATG CTGCTGCTGT GGGTAATTAT 
GTTGCCCAAT TGCTGCAGTC TATAGGCCAG GCACCAGAGT CCATTTCAGA GAAAGAATTA 
AAATTACTCT GCAGCAATTC TGCATTTCTT CAAGTGGTAA GATGTCGATC CTTAGCTGAA 
GAATATGGCT TAGATACAAT TAACAAGGAT GAAATTATTT CCAGCATGGA CAATCCAGAT 
AATGAGATAG TATTATACTT GATGTTACGG GCTGTTGATA GATTTTATAA ACAACACGGT 
AGATATCCAG GAGTATCTAA CTATCAAGTT GAAGAAGATG TAGGAAAACT GAAGTCTTGT 
CTCACTGGCT TCCTTCAGGA ACATGGATTA TCTGTAATGG TGAAAGATGA TTATGTCCAT 
GAATTTTGCC GATATGGAGC TGCTGAGCCA CACACCATTG CTGCTTTCTT AGGGGGAGCT 
GCTGCTCAAG AGGTTATCAA AGTAATTACA AAACAATTTG TAATTTTTAA TAATACATAC 
ATTTATAGTG GCATGTCACA AACTTCAGCA ACTTTCCAGT TGTAA