NEMF cDNA ORF clone, Lonchura striata domestica(Bengalese finch)

Gene
Clones
gRNAs

Summary

Gene Symbol	NEMF
Entrez Gene ID	110473494
Full Name	nuclear export mediator factor
Gene Type	protein-coding
Organism	Lonchura striata domestica(Bengalese finch)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021536064.1	XP_021391739.1	nuclear export mediator factor NEMF isoform X1
XM_021536065.1	XP_021391740.1	nuclear export mediator factor NEMF isoform X2

Pathways from BioSystems
Gene Ontology

The following NEMF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEMF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLo91184	XM_021536064.1 Latest version!	Lonchura striata domestica nuclear export mediator factor (NEMF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OLo91185	XM_021536065.1 Latest version!	Lonchura striata domestica nuclear export mediator factor (NEMF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NEMF ( XM_021536064.1 ) cDNA ORF clone, Lonchura striata domestica(Bengalese finch) -> XP_021391739.1 Lonchura striata domestica nuclear export mediator factor (NEMF), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OLo91184
Clone ID Related Accession (Same CDS sequence)	XM_021536064.1
Accession Version	XM_021536064.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Lonchura striata domestica(Bengalese finch)
Product	nuclear export mediator factor NEMF isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042571.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Lonchura striata domestica Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTCCC GCTTCAGCAC CGTCGACATC CGCGCGGTGC TCGCCGAGCT GCGGCAGAGC 
TTGTTGGGAA TGAGAGTGAA CAATGTTTAT GATGTGGATA ACAAGACCTA TCTCATCCGC 
CTGCAAAAGC CGGAGTGCAA GGCCACGCTG CTGCTGGAGT CCGGCATCCG CATCCACCTG 
ACGGAGTTCG AGTGGCCCAA GAACATGATG CCCTCCAGCT TTGCCATGAA GTGCCGGAAG 
CACCTGAGGA CGCGGCGCCT GGTGAGCGTG CGGCAGCTGG GCGTGGACCG CGTCGTGGAC 
CTGCAGTTCG GCAGCGAGCA GGCCGCCTAC CACCTGATCC TGGAGCTCTA CGACAGGGGC 
AACGTCGTCC TCACCGACCA CGAGTACCTG ATCCTCAACA TCCTGAGGTT CCGCACCGAC 
GAGGCCGACG ACGTCCGCTT CGCCGTCCGG GAGCGGTACC CGGTGGAGAG CGCGAAAGCT 
GCTGCGCCCC TGCCCACCCT GGACAGGTTA ACTGAAATCA TCTCAAATGC ACCCAAAGGG 
GAGCAGCTGA AGAGGGTCCT TAACCCACTC CTTCCTTATG GGTCCTCTCT CATCGAGCAC 
TGCCTTATAG AAGCTGGATT TTCTGGAGCT GTCAAAATAG ATCAACATCT GGAAAAGAAA 
GAAAACCTTG AAAAGGTTCT TTCAGCTCTA GAGAAAGCAG AAGAATACAT GGCCCTCACT 
GACAACTTCA GTGGGAAGGG TTACGTTATC CAGAAAAGGG AGAAAAAGCC AAGTCTGGAA 
CCAGATAAAC CAGCAGAAGA TATCTACACA TATGAGGAAT TTCATCCTTT CTTGTTTTCT 
CAACATTCAA AATGTCCCTA CTTGGAATTT GACTCATTCA ATAAGGCAAC AGATGAGTTC 
TACTCCAAGC TGGAGGGGCA GAAGATTGAT CTGAAGGCCT TGCAGCAGGA AAAACAAGCC 
TTGAAGAAAC TTGAAAATGT CCGCAGGGAC CATGAGCACA GACTGGAAGC TCTCCAGCAG 
GCTCAGGAAG CTGATAAGCT GAAGGGAGAG CTGATAGAGA TGAACCTGGA GGTGGTGGAC 
AGGGCCATCC AGGTGGTCCG CAGCGCCCTG GCCAACCAGA TCGACTGGAC CGAGATTGGC 
GCCATCGTCA AGGAGGCCCA GGCCCAGGGG GACCCCGTGG CCACGGCAAT CAAAGAGTTA 
AAGCTGCAGA CAAACCACAT CACCATGCTG CTGAGGAACC CCTATGTGTT ATCTGATGAG 
GAAGAGGAGG AGGACGATGC TGACGTAGAG AAAGAAGAAA CAGAAGAACC AAAGGGAAAA 
AAGAAAAAGA ATAAAACCAA ACAGTTGAAG AAACCTCAGA AAAACAAACC CTCATTGGTT 
GATGTTGATC TCAATTTGTC TGCTTATGCC AATGCCAAAA AGTACTATGA TCACAAAAGA 
CATGCAGCTA AGAAAACTCA GAAGACAGTG GAAGCTGCAG AAAAGGCTTT TAAATCAGCT 
GAGAAGAAGA CAAAGCAAAC CCTGAGGGAA GTTCAGACTG TCACCACCAT CCAGAAGGCC 
AGAAAGGTCT ATTGGTTTGA GAAGTTCCTG TGGTTCATCA GCTCTGAGAA TTACCTGGTG 
ATTGCTGGCA GGGATCAGCA GCAGAACGAG CTGATCGTGA AGCGTTACCT CAAACCAGGG 
GACATCTACG TGCACGCAGA TCTGCATGGA GCCACCAGCT GCGTGATCAA GAACCCCTCA 
GGTGAGCCCA TCCCGCCGCG GACGCTGACG GAGGCGGGGA CCATGGCCCT GTGCTACAGC 
GCGGCCTGGG ACGCCCGCGT GGTCACCAGC GCCTGGTGGG TGTCCCACAG CCAGGTTTCT 
AAAACTGCCC CCACGGGAGA ATATCTCACT ACTGGCAGCT TCATGATCCG AGGGAAAAAG 
AATTTCCTTC CACCTTCCTA TCTGATGATG GGCTTCAGCT TCTTGTTTAA GGTGGATGAG 
AGCTGTGTCT GGAGGCACCG GGAGGAGAGG AAGGTGAAAG TCCAGGATGA GGATCTGGAC 
ACGGTTTCCA GCAGTGCCAG TGAGCTGGTG GCTGAGGAGG TGGAGCTTTT AGATGGAGGA 
GACAGCAGCA GTGAGGAGGA AAAAGCCGAG GCTGAGGAAG CTCCAGAGGC TGAGGAAGCT 
CCAGAGGCTG AGGAAGCTCC AGAGGCTGAG GAAGCTCCAG AGGCTGAGGA AGCCACAGCT 
GAGGGCTGCC ATGAGGAGGG CATTGCTGAG CAGGGAGGAG GGAACACTCC TGCTCCAGAG 
GCTGAATCCG AGGAGGACGA CGGAGACTCT GAGGAGGAAC ATCCAGAGCC CCAGAGGGAA 
GTGAAGGAGG AAGAGGTGAA TTATCCAGAC ACCACAATTG ACCTGTCACA CCTTCAGCCC 
CAGAGATCCC TGCAGAAGAT CATCCCCAAA GAGGAAGAGC CCAACTTGGG TGACAGCAGG 
GCCCAGGGCC GGAGGCATCT CTCTGCCAAG GAGAGGAGAG AAATGAAGAA AAAGAAGCAG 
CAGAACAACT CTGAGAACTC GGAGCTGTCT GAAGAGAAGC AGAAGGAGAC AGAGACAGAG 
ACCCAACCTC TGGCTGCTCC CAACTGCCAC AAGGCAGCTC CAGCCCCTCA GCCCATCAAG 
CGGGGCCAGA AGAGTAAAAT GAAGAAGATG AAGGAGAAGT ACAGGGACCA GGACGAGGAG 
GACCGGGAGC TCATCATGAA GCTGCTGGGG TCTGCAGGCT CCAACAGGGA GGACAAAGGG 
AAAAAAGGGA AGAAGGGGAA GACAAAAGAA GAGGCAGCAA AGAAGCAACA GCAGAAAAGC 
AAACCCCTGC GTCGTGCAGC AGGAGGGGGC AAGGAGTCCC TCCCAGCAGG AATTGTGCTG 
CACGAGGCAC AGGAGCCAGG CCTGGATGAG CTGCAGGAGG ACAAGGAGGA GCAGGACCAG 
GAGCAGCCAG GACTGGAGGA GAGCGAGGCC CTGCTGGACT CGCTGACCGG GCAGCCCCAC 
CCCGAGGACA TCCTGCTCTT TGCTGTGCCC ATCTGTGCTC CCTACACAGC CATGGCCAAC 
TACAAGTACA AAGTCAAGCT CACTCCGGGC ACCCAGAAGA AGGGCAAAGC TGCCAAGATT 
GCCTTGCACA ATTTTATGCA GTCCAAAGAA GCCAGTGCAC GAGAGAAGGA CCTGTTCCGC 
AGCGTGAAGG ACACAGATTT GTCAAGAAAT ATTCCTGGGA AGGTGAAAGT GTCTGCACCT 
CACCTCCAGA ACATGAAGAG GAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021391739.1
CDS	111..3377
Translation	MKSRFSTVDIRAVLAELRQSLLGMRVNNVYDVDNKTYLIRLQKPECKATLLLESGIRIHLTEFEWPKNMMPSSFAMKCRKHLRTRRLVSVRQLGVDRVVDLQFGSEQAAYHLILELYDRGNVVLTDHEYLILNILRFRTDEADDVRFAVRERYPVESAKAAAPLPTLDRLTEIISNAPKGEQLKRVLNPLLPYGSSLIEHCLIEAGFSGAVKIDQHLEKKENLEKVLSALEKAEEYMALTDNFSGKGYVIQKREKKPSLEPDKPAEDIYTYEEFHPFLFSQHSKCPYLEFDSFNKATDEFYSKLEGQKIDLKALQQEKQALKKLENVRRDHEHRLEALQQAQEADKLKGELIEMNLEVVDRAIQVVRSALANQIDWTEIGAIVKEAQAQGDPVATAIKELKLQTNHITMLLRNPYVLSDEEEEEDDADVEKEETEEPKGKKKKNKTKQLKKPQKNKPSLVDVDLNLSAYANAKKYYDHKRHAAKKTQKTVEAAEKAFKSAEKKTKQTLREVQTVTTIQKARKVYWFEKFLWFISSENYLVIAGRDQQQNELIVKRYLKPGDIYVHADLHGATSCVIKNPSGEPIPPRTLTEAGTMALCYSAAWDARVVTSAWWVSHSQVSKTAPTGEYLTTGSFMIRGKKNFLPPSYLMMGFSFLFKVDESCVWRHREERKVKVQDEDLDTVSSSASELVAEEVELLDGGDSSSEEEKAEAEEAPEAEEAPEAEEAPEAEEAPEAEEATAEGCHEEGIAEQGGGNTPAPEAESEEDDGDSEEEHPEPQREVKEEEVNYPDTTIDLSHLQPQRSLQKIIPKEEEPNLGDSRAQGRRHLSAKERREMKKKKQQNNSENSELSEEKQKETETETQPLAAPNCHKAAPAPQPIKRGQKSKMKKMKEKYRDQDEEDRELIMKLLGSAGSNREDKGKKGKKGKTKEEAAKKQQQKSKPLRRAAGGGKESLPAGIVLHEAQEPGLDELQEDKEEQDQEQPGLEESEALLDSLTGQPHPEDILLFAVPICAPYTAMANYKYKVKLTPGTQKKGKAAKIALHNFMQSKEASAREKDLFRSVKDTDLSRNIPGKVKVSAPHLQNMKRK

Target ORF information:

RefSeq Version	XM_021536064.1
Organism	Lonchura striata domestica(Bengalese finch)
Definition	Lonchura striata domestica nuclear export mediator factor (NEMF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021536064.1

ORF Insert Sequence:

ATGAAGTCCC GCTTCAGCAC CGTCGACATC CGCGCGGTGC TCGCCGAGCT GCGGCAGAGC 
TTGTTGGGAA TGAGAGTGAA CAATGTTTAT GATGTGGATA ACAAGACCTA TCTCATCCGC 
CTGCAAAAGC CGGAGTGCAA GGCCACGCTG CTGCTGGAGT CCGGCATCCG CATCCACCTG 
ACGGAGTTCG AGTGGCCCAA GAACATGATG CCCTCCAGCT TTGCCATGAA GTGCCGGAAG 
CACCTGAGGA CGCGGCGCCT GGTGAGCGTG CGGCAGCTGG GCGTGGACCG CGTCGTGGAC 
CTGCAGTTCG GCAGCGAGCA GGCCGCCTAC CACCTGATCC TGGAGCTCTA CGACAGGGGC 
AACGTCGTCC TCACCGACCA CGAGTACCTG ATCCTCAACA TCCTGAGGTT CCGCACCGAC 
GAGGCCGACG ACGTCCGCTT CGCCGTCCGG GAGCGGTACC CGGTGGAGAG CGCGAAAGCT 
GCTGCGCCCC TGCCCACCCT GGACAGGTTA ACTGAAATCA TCTCAAATGC ACCCAAAGGG 
GAGCAGCTGA AGAGGGTCCT TAACCCACTC CTTCCTTATG GGTCCTCTCT CATCGAGCAC 
TGCCTTATAG AAGCTGGATT TTCTGGAGCT GTCAAAATAG ATCAACATCT GGAAAAGAAA 
GAAAACCTTG AAAAGGTTCT TTCAGCTCTA GAGAAAGCAG AAGAATACAT GGCCCTCACT 
GACAACTTCA GTGGGAAGGG TTACGTTATC CAGAAAAGGG AGAAAAAGCC AAGTCTGGAA 
CCAGATAAAC CAGCAGAAGA TATCTACACA TATGAGGAAT TTCATCCTTT CTTGTTTTCT 
CAACATTCAA AATGTCCCTA CTTGGAATTT GACTCATTCA ATAAGGCAAC AGATGAGTTC 
TACTCCAAGC TGGAGGGGCA GAAGATTGAT CTGAAGGCCT TGCAGCAGGA AAAACAAGCC 
TTGAAGAAAC TTGAAAATGT CCGCAGGGAC CATGAGCACA GACTGGAAGC TCTCCAGCAG 
GCTCAGGAAG CTGATAAGCT GAAGGGAGAG CTGATAGAGA TGAACCTGGA GGTGGTGGAC 
AGGGCCATCC AGGTGGTCCG CAGCGCCCTG GCCAACCAGA TCGACTGGAC CGAGATTGGC 
GCCATCGTCA AGGAGGCCCA GGCCCAGGGG GACCCCGTGG CCACGGCAAT CAAAGAGTTA 
AAGCTGCAGA CAAACCACAT CACCATGCTG CTGAGGAACC CCTATGTGTT ATCTGATGAG 
GAAGAGGAGG AGGACGATGC TGACGTAGAG AAAGAAGAAA CAGAAGAACC AAAGGGAAAA 
AAGAAAAAGA ATAAAACCAA ACAGTTGAAG AAACCTCAGA AAAACAAACC CTCATTGGTT 
GATGTTGATC TCAATTTGTC TGCTTATGCC AATGCCAAAA AGTACTATGA TCACAAAAGA 
CATGCAGCTA AGAAAACTCA GAAGACAGTG GAAGCTGCAG AAAAGGCTTT TAAATCAGCT 
GAGAAGAAGA CAAAGCAAAC CCTGAGGGAA GTTCAGACTG TCACCACCAT CCAGAAGGCC 
AGAAAGGTCT ATTGGTTTGA GAAGTTCCTG TGGTTCATCA GCTCTGAGAA TTACCTGGTG 
ATTGCTGGCA GGGATCAGCA GCAGAACGAG CTGATCGTGA AGCGTTACCT CAAACCAGGG 
GACATCTACG TGCACGCAGA TCTGCATGGA GCCACCAGCT GCGTGATCAA GAACCCCTCA 
GGTGAGCCCA TCCCGCCGCG GACGCTGACG GAGGCGGGGA CCATGGCCCT GTGCTACAGC 
GCGGCCTGGG ACGCCCGCGT GGTCACCAGC GCCTGGTGGG TGTCCCACAG CCAGGTTTCT 
AAAACTGCCC CCACGGGAGA ATATCTCACT ACTGGCAGCT TCATGATCCG AGGGAAAAAG 
AATTTCCTTC CACCTTCCTA TCTGATGATG GGCTTCAGCT TCTTGTTTAA GGTGGATGAG 
AGCTGTGTCT GGAGGCACCG GGAGGAGAGG AAGGTGAAAG TCCAGGATGA GGATCTGGAC 
ACGGTTTCCA GCAGTGCCAG TGAGCTGGTG GCTGAGGAGG TGGAGCTTTT AGATGGAGGA 
GACAGCAGCA GTGAGGAGGA AAAAGCCGAG GCTGAGGAAG CTCCAGAGGC TGAGGAAGCT 
CCAGAGGCTG AGGAAGCTCC AGAGGCTGAG GAAGCTCCAG AGGCTGAGGA AGCCACAGCT 
GAGGGCTGCC ATGAGGAGGG CATTGCTGAG CAGGGAGGAG GGAACACTCC TGCTCCAGAG 
GCTGAATCCG AGGAGGACGA CGGAGACTCT GAGGAGGAAC ATCCAGAGCC CCAGAGGGAA 
GTGAAGGAGG AAGAGGTGAA TTATCCAGAC ACCACAATTG ACCTGTCACA CCTTCAGCCC 
CAGAGATCCC TGCAGAAGAT CATCCCCAAA GAGGAAGAGC CCAACTTGGG TGACAGCAGG 
GCCCAGGGCC GGAGGCATCT CTCTGCCAAG GAGAGGAGAG AAATGAAGAA AAAGAAGCAG 
CAGAACAACT CTGAGAACTC GGAGCTGTCT GAAGAGAAGC AGAAGGAGAC AGAGACAGAG 
ACCCAACCTC TGGCTGCTCC CAACTGCCAC AAGGCAGCTC CAGCCCCTCA GCCCATCAAG 
CGGGGCCAGA AGAGTAAAAT GAAGAAGATG AAGGAGAAGT ACAGGGACCA GGACGAGGAG 
GACCGGGAGC TCATCATGAA GCTGCTGGGG TCTGCAGGCT CCAACAGGGA GGACAAAGGG 
AAAAAAGGGA AGAAGGGGAA GACAAAAGAA GAGGCAGCAA AGAAGCAACA GCAGAAAAGC 
AAACCCCTGC GTCGTGCAGC AGGAGGGGGC AAGGAGTCCC TCCCAGCAGG AATTGTGCTG 
CACGAGGCAC AGGAGCCAGG CCTGGATGAG CTGCAGGAGG ACAAGGAGGA GCAGGACCAG 
GAGCAGCCAG GACTGGAGGA GAGCGAGGCC CTGCTGGACT CGCTGACCGG GCAGCCCCAC 
CCCGAGGACA TCCTGCTCTT TGCTGTGCCC ATCTGTGCTC CCTACACAGC CATGGCCAAC 
TACAAGTACA AAGTCAAGCT CACTCCGGGC ACCCAGAAGA AGGGCAAAGC TGCCAAGATT 
GCCTTGCACA ATTTTATGCA GTCCAAAGAA GCCAGTGCAC GAGAGAAGGA CCTGTTCCGC 
AGCGTGAAGG ACACAGATTT GTCAAGAAAT ATTCCTGGGA AGGTGAAAGT GTCTGCACCT 
CACCTCCAGA ACATGAAGAG GAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEMF ( XM_021536065.1 ) cDNA ORF clone, Lonchura striata domestica(Bengalese finch) -> XP_021391740.1 Lonchura striata domestica nuclear export mediator factor (NEMF), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OLo91185
Clone ID Related Accession (Same CDS sequence)	XM_021536065.1
Accession Version	XM_021536065.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Lonchura striata domestica(Bengalese finch)
Product	nuclear export mediator factor NEMF isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042571.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Lonchura striata domestica Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTCCC GCTTCAGCAC CGTCGACATC CGCGCGGTGC TCGCCGAGCT GCGGCAGAGC 
TTGTTGGGAA TGAGAGTGAA CAATGTTTAT GATGTGGATA ACAAGACCTA TCTCATCCGC 
CTGCAAAAGC CGGAGTGCAA GGCCACGCTG CTGCTGGAGT CCGGCATCCG CATCCACCTG 
ACGGAGTTCG AGTGGCCCAA GAACATGATG CCCTCCAGCT TTGCCATGAA GTGCCGGAAG 
CACCTGAGGA CGCGGCGCCT GGTGAGCGTG CGGCAGCTGG GCGTGGACCG CGTCGTGGAC 
CTGCAGTTCG GCAGCGAGCA GGCCGCCTAC CACCTGATCC TGGAGCTCTA CGACAGGGGC 
AACGTCGTCC TCACCGACCA CGAGTACCTG ATCCTCAACA TCCTGAGGTT CCGCACCGAC 
GAGGCCGACG ACGTCCGCTT CGCCGTCCGG GAGCGGTACC CGGTGGAGAG CGCGAAAGCT 
GCTGCGCCCC TGCCCACCCT GGACAGGTTA ACTGAAATCA TCTCAAATGC ACCCAAAGGG 
GAGCAGCTGA AGAGGGTCCT TAACCCACTC CTTCCTTATG GGTCCTCTCT CATCGAGCAC 
TGCCTTATAG AAGCTGGATT TTCTGGAGCT GTCAAAATAG ATCAACATCT GGAAAAGAAA 
GAAAACCTTG AAAAGGTTCT TTCAGCTCTA GAGAAAGCAG AAGAATACAT GGCCCTCACT 
GACAACTTCA GTGGGAAGGG TTACGTTATC CAGAAAAGGG AGAAAAAGCC AAGTCTGGAA 
CCAGATAAAC CAGCAGAAGA TATCTACACA TATGAGGAAT TTCATCCTTT CTTGTTTTCT 
CAACATTCAA AATGTCCCTA CTTGGAATTT GACTCATTCA ATAAGGCAAC AGATGAGTTC 
TACTCCAAGC TGGAGGGGCA GAAGATTGAT CTGAAGGCCT TGCAGCAGGA AAAACAAGCC 
TTGAAGAAAC TTGAAAATGT CCGCAGGGAC CATGAGCACA GACTGGAAGC TCTCCAGCAG 
GCTCAGGAAG CTGATAAGCT GAAGGGAGAG CTGATAGAGA TGAACCTGGA GGTGGTGGAC 
AGGGCCATCC AGGTGGTCCG CAGCGCCCTG GCCAACCAGA TCGACTGGAC CGAGATTGGC 
GCCATCGTCA AGGAGGCCCA GGCCCAGGGG GACCCCGTGG CCACGGCAAT CAAAGAGTTA 
AAGCTGCAGA CAAACCACAT CACCATGCTG CTGAGGAACC CCTATGTGTT ATCTGATGAG 
GAAGAGGAGG AGGACGATGC TGACGTAGAG AAAGAAGAAA CAGAAGAACC AAAGGGAAAA 
AAGAAAAAGA ATAAAACCAA ACAGTTGAAG AAACCTCAGA AAAACAAACC CTCATTGGTT 
GATGTTGATC TCAATTTGTC TGCTTATGCC AATGCCAAAA AGTACTATGA TCACAAAAGA 
CATGCAGCTA AGAAAACTCA GAAGACAGTG GAAGCTGCAG AAAAGGCTTT TAAATCAGCT 
GAGAAGAAGA CAAAGCAAAC CCTGAGGGAA GTTCAGACTG TCACCACCAT CCAGAAGGCC 
AGAAAGGTCT ATTGGTTTGA GAAGTTCCTG TGGTTCATCA GCTCTGAGAA TTACCTGGTG 
ATTGCTGGCA GGGATCAGCA GCAGAACGAG CTGATCGTGA AGCGTTACCT CAAACCAGGG 
GACATCTACG TGCACGCAGA TCTGCATGGA GCCACCAGCT GCGTGATCAA GAACCCCTCA 
GGTGAGCCCA TCCCGCCGCG GACGCTGACG GAGGCGGGGA CCATGGCCCT GTGCTACAGC 
GCGGCCTGGG ACGCCCGCGT GGTCACCAGC GCCTGGTGGG TGTCCCACAG CCAGGTTTCT 
AAAACTGCCC CCACGGGAGA ATATCTCACT ACTGGCAGCT TCATGATCCG AGGGAAAAAG 
AATTTCCTTC CACCTTCCTA TCTGATGATG GGCTTCAGCT TCTTGTTTAA GGTGGATGAG 
AGCTGTGTCT GGAGGCACCG GGAGGAGAGG AAGGTGAAAG TCCAGGATGA GGATCTGGAC 
ACGGTTTCCA GCAGTGCCAG TGAGCTGGTG GCTGAGGAGG TGGAGCTTTT AGATGGAGGA 
GACAGCAGCA GTGAGGAGGA AAAAGCCGAG GCTGAGGAAG CTCCAGAGGC TGAGGAAGCT 
CCAGAGGCTG AGGAAGCCAC AGCTGAGGGC TGCCATGAGG AGGGCATTGC TGAGCAGGGA 
GGAGGGAACA CTCCTGCTCC AGAGGCTGAA TCCGAGGAGG ACGACGGAGA CTCTGAGGAG 
GAACATCCAG AGCCCCAGAG GGAAGTGAAG GAGGAAGAGG TGAATTATCC AGACACCACA 
ATTGACCTGT CACACCTTCA GCCCCAGAGA TCCCTGCAGA AGATCATCCC CAAAGAGGAA 
GAGCCCAACT TGGGTGACAG CAGGGCCCAG GGCCGGAGGC ATCTCTCTGC CAAGGAGAGG 
AGAGAAATGA AGAAAAAGAA GCAGCAGAAC AACTCTGAGA ACTCGGAGCT GTCTGAAGAG 
AAGCAGAAGG AGACAGAGAC AGAGACCCAA CCTCTGGCTG CTCCCAACTG CCACAAGGCA 
GCTCCAGCCC CTCAGCCCAT CAAGCGGGGC CAGAAGAGTA AAATGAAGAA GATGAAGGAG 
AAGTACAGGG ACCAGGACGA GGAGGACCGG GAGCTCATCA TGAAGCTGCT GGGGTCTGCA 
GGCTCCAACA GGGAGGACAA AGGGAAAAAA GGGAAGAAGG GGAAGACAAA AGAAGAGGCA 
GCAAAGAAGC AACAGCAGAA AAGCAAACCC CTGCGTCGTG CAGCAGGAGG GGGCAAGGAG 
TCCCTCCCAG CAGGAATTGT GCTGCACGAG GCACAGGAGC CAGGCCTGGA TGAGCTGCAG 
GAGGACAAGG AGGAGCAGGA CCAGGAGCAG CCAGGACTGG AGGAGAGCGA GGCCCTGCTG 
GACTCGCTGA CCGGGCAGCC CCACCCCGAG GACATCCTGC TCTTTGCTGT GCCCATCTGT 
GCTCCCTACA CAGCCATGGC CAACTACAAG TACAAAGTCA AGCTCACTCC GGGCACCCAG 
AAGAAGGGCA AAGCTGCCAA GATTGCCTTG CACAATTTTA TGCAGTCCAA AGAAGCCAGT 
GCACGAGAGA AGGACCTGTT CCGCAGCGTG AAGGACACAG ATTTGTCAAG AAATATTCCT 
GGGAAGGTGA AAGTGTCTGC ACCTCACCTC CAGAACATGA AGAGGAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021391740.1
CDS	111..3341
Translation	MKSRFSTVDIRAVLAELRQSLLGMRVNNVYDVDNKTYLIRLQKPECKATLLLESGIRIHLTEFEWPKNMMPSSFAMKCRKHLRTRRLVSVRQLGVDRVVDLQFGSEQAAYHLILELYDRGNVVLTDHEYLILNILRFRTDEADDVRFAVRERYPVESAKAAAPLPTLDRLTEIISNAPKGEQLKRVLNPLLPYGSSLIEHCLIEAGFSGAVKIDQHLEKKENLEKVLSALEKAEEYMALTDNFSGKGYVIQKREKKPSLEPDKPAEDIYTYEEFHPFLFSQHSKCPYLEFDSFNKATDEFYSKLEGQKIDLKALQQEKQALKKLENVRRDHEHRLEALQQAQEADKLKGELIEMNLEVVDRAIQVVRSALANQIDWTEIGAIVKEAQAQGDPVATAIKELKLQTNHITMLLRNPYVLSDEEEEEDDADVEKEETEEPKGKKKKNKTKQLKKPQKNKPSLVDVDLNLSAYANAKKYYDHKRHAAKKTQKTVEAAEKAFKSAEKKTKQTLREVQTVTTIQKARKVYWFEKFLWFISSENYLVIAGRDQQQNELIVKRYLKPGDIYVHADLHGATSCVIKNPSGEPIPPRTLTEAGTMALCYSAAWDARVVTSAWWVSHSQVSKTAPTGEYLTTGSFMIRGKKNFLPPSYLMMGFSFLFKVDESCVWRHREERKVKVQDEDLDTVSSSASELVAEEVELLDGGDSSSEEEKAEAEEAPEAEEAPEAEEATAEGCHEEGIAEQGGGNTPAPEAESEEDDGDSEEEHPEPQREVKEEEVNYPDTTIDLSHLQPQRSLQKIIPKEEEPNLGDSRAQGRRHLSAKERREMKKKKQQNNSENSELSEEKQKETETETQPLAAPNCHKAAPAPQPIKRGQKSKMKKMKEKYRDQDEEDRELIMKLLGSAGSNREDKGKKGKKGKTKEEAAKKQQQKSKPLRRAAGGGKESLPAGIVLHEAQEPGLDELQEDKEEQDQEQPGLEESEALLDSLTGQPHPEDILLFAVPICAPYTAMANYKYKVKLTPGTQKKGKAAKIALHNFMQSKEASAREKDLFRSVKDTDLSRNIPGKVKVSAPHLQNMKRK

Target ORF information:

RefSeq Version	XM_021536065.1
Organism	Lonchura striata domestica(Bengalese finch)
Definition	Lonchura striata domestica nuclear export mediator factor (NEMF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021536065.1

ORF Insert Sequence:

ATGAAGTCCC GCTTCAGCAC CGTCGACATC CGCGCGGTGC TCGCCGAGCT GCGGCAGAGC 
TTGTTGGGAA TGAGAGTGAA CAATGTTTAT GATGTGGATA ACAAGACCTA TCTCATCCGC 
CTGCAAAAGC CGGAGTGCAA GGCCACGCTG CTGCTGGAGT CCGGCATCCG CATCCACCTG 
ACGGAGTTCG AGTGGCCCAA GAACATGATG CCCTCCAGCT TTGCCATGAA GTGCCGGAAG 
CACCTGAGGA CGCGGCGCCT GGTGAGCGTG CGGCAGCTGG GCGTGGACCG CGTCGTGGAC 
CTGCAGTTCG GCAGCGAGCA GGCCGCCTAC CACCTGATCC TGGAGCTCTA CGACAGGGGC 
AACGTCGTCC TCACCGACCA CGAGTACCTG ATCCTCAACA TCCTGAGGTT CCGCACCGAC 
GAGGCCGACG ACGTCCGCTT CGCCGTCCGG GAGCGGTACC CGGTGGAGAG CGCGAAAGCT 
GCTGCGCCCC TGCCCACCCT GGACAGGTTA ACTGAAATCA TCTCAAATGC ACCCAAAGGG 
GAGCAGCTGA AGAGGGTCCT TAACCCACTC CTTCCTTATG GGTCCTCTCT CATCGAGCAC 
TGCCTTATAG AAGCTGGATT TTCTGGAGCT GTCAAAATAG ATCAACATCT GGAAAAGAAA 
GAAAACCTTG AAAAGGTTCT TTCAGCTCTA GAGAAAGCAG AAGAATACAT GGCCCTCACT 
GACAACTTCA GTGGGAAGGG TTACGTTATC CAGAAAAGGG AGAAAAAGCC AAGTCTGGAA 
CCAGATAAAC CAGCAGAAGA TATCTACACA TATGAGGAAT TTCATCCTTT CTTGTTTTCT 
CAACATTCAA AATGTCCCTA CTTGGAATTT GACTCATTCA ATAAGGCAAC AGATGAGTTC 
TACTCCAAGC TGGAGGGGCA GAAGATTGAT CTGAAGGCCT TGCAGCAGGA AAAACAAGCC 
TTGAAGAAAC TTGAAAATGT CCGCAGGGAC CATGAGCACA GACTGGAAGC TCTCCAGCAG 
GCTCAGGAAG CTGATAAGCT GAAGGGAGAG CTGATAGAGA TGAACCTGGA GGTGGTGGAC 
AGGGCCATCC AGGTGGTCCG CAGCGCCCTG GCCAACCAGA TCGACTGGAC CGAGATTGGC 
GCCATCGTCA AGGAGGCCCA GGCCCAGGGG GACCCCGTGG CCACGGCAAT CAAAGAGTTA 
AAGCTGCAGA CAAACCACAT CACCATGCTG CTGAGGAACC CCTATGTGTT ATCTGATGAG 
GAAGAGGAGG AGGACGATGC TGACGTAGAG AAAGAAGAAA CAGAAGAACC AAAGGGAAAA 
AAGAAAAAGA ATAAAACCAA ACAGTTGAAG AAACCTCAGA AAAACAAACC CTCATTGGTT 
GATGTTGATC TCAATTTGTC TGCTTATGCC AATGCCAAAA AGTACTATGA TCACAAAAGA 
CATGCAGCTA AGAAAACTCA GAAGACAGTG GAAGCTGCAG AAAAGGCTTT TAAATCAGCT 
GAGAAGAAGA CAAAGCAAAC CCTGAGGGAA GTTCAGACTG TCACCACCAT CCAGAAGGCC 
AGAAAGGTCT ATTGGTTTGA GAAGTTCCTG TGGTTCATCA GCTCTGAGAA TTACCTGGTG 
ATTGCTGGCA GGGATCAGCA GCAGAACGAG CTGATCGTGA AGCGTTACCT CAAACCAGGG 
GACATCTACG TGCACGCAGA TCTGCATGGA GCCACCAGCT GCGTGATCAA GAACCCCTCA 
GGTGAGCCCA TCCCGCCGCG GACGCTGACG GAGGCGGGGA CCATGGCCCT GTGCTACAGC 
GCGGCCTGGG ACGCCCGCGT GGTCACCAGC GCCTGGTGGG TGTCCCACAG CCAGGTTTCT 
AAAACTGCCC CCACGGGAGA ATATCTCACT ACTGGCAGCT TCATGATCCG AGGGAAAAAG 
AATTTCCTTC CACCTTCCTA TCTGATGATG GGCTTCAGCT TCTTGTTTAA GGTGGATGAG 
AGCTGTGTCT GGAGGCACCG GGAGGAGAGG AAGGTGAAAG TCCAGGATGA GGATCTGGAC 
ACGGTTTCCA GCAGTGCCAG TGAGCTGGTG GCTGAGGAGG TGGAGCTTTT AGATGGAGGA 
GACAGCAGCA GTGAGGAGGA AAAAGCCGAG GCTGAGGAAG CTCCAGAGGC TGAGGAAGCT 
CCAGAGGCTG AGGAAGCCAC AGCTGAGGGC TGCCATGAGG AGGGCATTGC TGAGCAGGGA 
GGAGGGAACA CTCCTGCTCC AGAGGCTGAA TCCGAGGAGG ACGACGGAGA CTCTGAGGAG 
GAACATCCAG AGCCCCAGAG GGAAGTGAAG GAGGAAGAGG TGAATTATCC AGACACCACA 
ATTGACCTGT CACACCTTCA GCCCCAGAGA TCCCTGCAGA AGATCATCCC CAAAGAGGAA 
GAGCCCAACT TGGGTGACAG CAGGGCCCAG GGCCGGAGGC ATCTCTCTGC CAAGGAGAGG 
AGAGAAATGA AGAAAAAGAA GCAGCAGAAC AACTCTGAGA ACTCGGAGCT GTCTGAAGAG 
AAGCAGAAGG AGACAGAGAC AGAGACCCAA CCTCTGGCTG CTCCCAACTG CCACAAGGCA 
GCTCCAGCCC CTCAGCCCAT CAAGCGGGGC CAGAAGAGTA AAATGAAGAA GATGAAGGAG 
AAGTACAGGG ACCAGGACGA GGAGGACCGG GAGCTCATCA TGAAGCTGCT GGGGTCTGCA 
GGCTCCAACA GGGAGGACAA AGGGAAAAAA GGGAAGAAGG GGAAGACAAA AGAAGAGGCA 
GCAAAGAAGC AACAGCAGAA AAGCAAACCC CTGCGTCGTG CAGCAGGAGG GGGCAAGGAG 
TCCCTCCCAG CAGGAATTGT GCTGCACGAG GCACAGGAGC CAGGCCTGGA TGAGCTGCAG 
GAGGACAAGG AGGAGCAGGA CCAGGAGCAG CCAGGACTGG AGGAGAGCGA GGCCCTGCTG 
GACTCGCTGA CCGGGCAGCC CCACCCCGAG GACATCCTGC TCTTTGCTGT GCCCATCTGT 
GCTCCCTACA CAGCCATGGC CAACTACAAG TACAAAGTCA AGCTCACTCC GGGCACCCAG 
AAGAAGGGCA AAGCTGCCAA GATTGCCTTG CACAATTTTA TGCAGTCCAA AGAAGCCAGT 
GCACGAGAGA AGGACCTGTT CCGCAGCGTG AAGGACACAG ATTTGTCAAG AAATATTCCT 
GGGAAGGTGA AAGTGTCTGC ACCTCACCTC CAGAACATGA AGAGGAAGTG A