NLRP3 cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin)

Gene
Clones
gRNAs

Summary

Gene Symbol	NLRP3
Entrez Gene ID	108508873
Full Name	NLR family pyrin domain containing 3
Gene Type	protein-coding
Organism	Lepidothrix coronata(blue-crowned manakin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017838015.1	XP_017693504.1	NACHT, LRR and PYD domains-containing protein 3

Pathways from BioSystems
Gene Ontology

The following NLRP3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NLRP3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLe74437	XM_017838015.1 Latest version!	Lepidothrix coronata NLR family pyrin domain containing 3 (NLRP3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NLRP3 ( XM_017838015.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017693504.1 Lepidothrix coronata NLR family pyrin domain containing 3 (NLRP3), mRNA.

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CloneID	OLe74437
Clone ID Related Accession (Same CDS sequence)	XM_017838015.1
Accession Version	XM_017838015.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2574bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	NACHT, LRR and PYD domains-containing protein 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690499.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGACG TTTCTCACAG TGGAGTGACT TGCTGCAGCA GCCAAGAGTA CAAGCGGAAC 
TACAGAGAGC ACATGGTCCG GGAGTTCCTC CGAGACGGAG CAGGGGCCTC CAGTCCCGGG 
GAGAACCCAT CCATCCGCAG CCACTACACC GCCCTGACCA TCGCCAGGGA GCCTGGGAGC 
GGGCACGGGG CTGAGCGTGG AGCTGTGGGA TGTGCCAGCA CCAGCAATGG ACCAGCCACC 
GTCACCGCAC AGACGCTGTT CAGACCCGAC GAGGACGGGC AAACACCGCG GGTCGTGGTG 
CTGGTGGGGG CCCCGGGGAT GGGGAAGACG ACGACGGTCA GGAAGGTGAT GGTGGAGTGG 
GGAGAAGGGA CCCTCCACGA GCAGTTTGAC TGTGTCTTCT GCATTGACTG TAAAGGCATC 
GCCCTTCCCA AGGAAGCGAG TGTGGCAGAC CTGGTTTCAC AGTGCTGCCC TCACCAGCGA 
GTGCCGGCTG GGGGGATCCT GGATGACCAG AAGATCCTGT TCGTTTTTGA TGGCTTCGAG 
GCCCTGGGAT TTTCGTTGGT TCAGCCTGAG GCTGAGCTGA GCTTTGACCC TAGGGAGGTG 
AAGCCGCTGG AAACCACCCT GATGAGCTTG TTGAAGGGGA CTGTTCTCCC AGAGGCTTCT 
TTGCTCATCA CCACGAGGCC AACGGCTCTT CAAAGCCTGG GGCAGTGCCT GGAGGGGGAG 
TATTACGCAG AGATACTGGG ATTTTCAGCA GCCATGAGGG AGGAATATTT CCACAGATAT 
TTTGAGAATG CCAACAAAGC TGACATGGCC TTCAGGTTTG CCAGAGGCAA TGAGATCCTC 
TACAGCTTGT GCGTCATCCC CGTCATGAGC TGGGCTGTCT GCACCATCCT TGAACAAGAG 
CTTTGCAGGA AGAAAAACCT CCTTGAGTGC TCCAAAGCCA CCACAAGGAT GACCATGTCT 
TACCTCTCCC AGTTACTGAA GCACAGGGAC AGGGACAATC CACAGGTCCT CCAGAGGTTC 
CTTCTCCGGC TTTGCTCCTT GGCTGCCGAT GGCATCTGGA AGCACAAGGT CCTCTTTGAG 
GAGAAGGAGA TCAGGGACCG TGGCTTGGGC CAGCCAGACC TTCTTCCCCT CTTCCTCAAC 
GAGAAAATCT CAAAGGAAGG TGGAGACCAC GGGAAGGTCT ACGGCTTCAC CCATCTGCAC 
CTCCAGGAGT TTTTTGCAGC GATGTTTTAT GTTCTGGAGG ACGATGAGGA AACGGTCAGT 
GACTTGGGGG GGCTTGAGAA GGATGTAAGT AAGTTACTAG AAAGCTACAG TGAGTCCAGG 
AAGGATTTGA ATGTAACAGT GCAGTTCCTC TTTGGTTTAG TCAGCCAAAA ATCCATGGAG 
TATGTGGACA AAACCATTGG GTGCAGGATT TCACCACAAG CCAGGGAAGA CTTGCTGAAG 
TGGCTTCAGG GGAGGCACAG GAGCATCTCC CACCCCAGCC AAGCGCTGAA GATTTCGGAG 
TTGGACACTT TCCACTTCTT GTTCGAGATG AATGAGAAGA GCTTTGCACA AAGTGCGTTG 
GGTCATTTCA GTGAGCTGGA CTTGCAGGAC ATGAAGTTGA CTCTCTACGA CCAAGTGGCA 
CTTTCCTTCT GCATCCAGCA GTGGGCTGGG CTGGGCTGTG TCACCCTCCG GGGATGCTCC 
TTCCACCAGC AGGATCCTAG GGTGGAGCTG GATGCGTTGG TGCCCTGCTC TGGAGCCTCG 
GGAGCTGGAG GACGTCCTGG TGCCACCGCT GAGCTGCGGC AGGAGGAGCT GCACTGCCCC 
ATCCACCTGC TGTGCCGGGC ACTGCGGCAC CCAGGCAGTG CCCTGCGGGT GCTCCGGCTG 
CAGTGGTGCC GGCTGTCCGA GAGCTGCTGC GCCGAGCTGG CGGCGCTGCT GGCGGAACAC 
CCCAGCCTGG CGCAGCTGGA GCTGGGCGAC GGCGCGCTGG GCGACCGCGG CGTGCGCCTG 
CTGTGCCAGG GTCTGCGGCG TCCTGGCTGC CGCCTGCGCG TCCTGCGGCT GCGTTATTCC 
CGCCTCACCA GCAGCTGCTG TGAGGACCTC GCTGCCGTGC TGGGCACCAG CCCGTGGCTG 
GAGGAGCTGG ATTTGTCCTT CAGCGAGGGG CTGCGCGATG CCGGGGTGCA GCTGCTGTGC 
CAGGGGCTCC AGCACCGCGC CTGCCCGCTG CAGACGCTGC GGCTGGGGAG CTGCCGGCTG 
ACGGGCGCCT GCTGCCCAGC CCTGGCTGCA CAGCTGCTGG AGGGGCCTCA TCTCACCTGC 
CTGGACCTGA GTGACAACGA TCTGGGAGCT GATGGCATCC TGCATCTCTG CCAGCAGCTC 
CGGCATCCCG CCTGCCCACT GCGGACCCTG GGGCTGAGCA CGGATGGGTT AAGCCAGGAC 
GTGCTGCAGG AACTGGCCGC TCTCCAGGCC CTGAAGCCCG ATCTGAAGAT CAGGAACCTC 
CTGGAGCATG ATGTGCCAGA GGTGGGGGCC ATGGCCCGGC TGCCCTGCCA CCGTGGGGTC 
CAGCCAGGCG GCAGGAAGGC AATTCCCTCC TTCAGGAGAG CTCCAATCCT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017693504.1
CDS	229..2802
Translation	MKDVSHSGVTCCSSQEYKRNYREHMVREFLRDGAGASSPGENPSIRSHYTALTIAREPGSGHGAERGAVGCASTSNGPATVTAQTLFRPDEDGQTPRVVVLVGAPGMGKTTTVRKVMVEWGEGTLHEQFDCVFCIDCKGIALPKEASVADLVSQCCPHQRVPAGGILDDQKILFVFDGFEALGFSLVQPEAELSFDPREVKPLETTLMSLLKGTVLPEASLLITTRPTALQSLGQCLEGEYYAEILGFSAAMREEYFHRYFENANKADMAFRFARGNEILYSLCVIPVMSWAVCTILEQELCRKKNLLECSKATTRMTMSYLSQLLKHRDRDNPQVLQRFLLRLCSLAADGIWKHKVLFEEKEIRDRGLGQPDLLPLFLNEKISKEGGDHGKVYGFTHLHLQEFFAAMFYVLEDDEETVSDLGGLEKDVSKLLESYSESRKDLNVTVQFLFGLVSQKSMEYVDKTIGCRISPQAREDLLKWLQGRHRSISHPSQALKISELDTFHFLFEMNEKSFAQSALGHFSELDLQDMKLTLYDQVALSFCIQQWAGLGCVTLRGCSFHQQDPRVELDALVPCSGASGAGGRPGATAELRQEELHCPIHLLCRALRHPGSALRVLRLQWCRLSESCCAELAALLAEHPSLAQLELGDGALGDRGVRLLCQGLRRPGCRLRVLRLRYSRLTSSCCEDLAAVLGTSPWLEELDLSFSEGLRDAGVQLLCQGLQHRACPLQTLRLGSCRLTGACCPALAAQLLEGPHLTCLDLSDNDLGADGILHLCQQLRHPACPLRTLGLSTDGLSQDVLQELAALQALKPDLKIRNLLEHDVPEVGAMARLPCHRGVQPGGRKAIPSFRRAPIL

Target ORF information:

RefSeq Version	XM_017838015.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata NLR family pyrin domain containing 3 (NLRP3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017838015.1

ORF Insert Sequence:

ATGAAGGACG TTTCTCACAG TGGAGTGACT TGCTGCAGCA GCCAAGAGTA CAAGCGGAAC 
TACAGAGAGC ACATGGTCCG GGAGTTCCTC CGAGACGGAG CAGGGGCCTC CAGTCCCGGG 
GAGAACCCAT CCATCCGCAG CCACTACACC GCCCTGACCA TCGCCAGGGA GCCTGGGAGC 
GGGCACGGGG CTGAGCGTGG AGCTGTGGGA TGTGCCAGCA CCAGCAATGG ACCAGCCACC 
GTCACCGCAC AGACGCTGTT CAGACCCGAC GAGGACGGGC AAACACCGCG GGTCGTGGTG 
CTGGTGGGGG CCCCGGGGAT GGGGAAGACG ACGACGGTCA GGAAGGTGAT GGTGGAGTGG 
GGAGAAGGGA CCCTCCACGA GCAGTTTGAC TGTGTCTTCT GCATTGACTG TAAAGGCATC 
GCCCTTCCCA AGGAAGCGAG TGTGGCAGAC CTGGTTTCAC AGTGCTGCCC TCACCAGCGA 
GTGCCGGCTG GGGGGATCCT GGATGACCAG AAGATCCTGT TCGTTTTTGA TGGCTTCGAG 
GCCCTGGGAT TTTCGTTGGT TCAGCCTGAG GCTGAGCTGA GCTTTGACCC TAGGGAGGTG 
AAGCCGCTGG AAACCACCCT GATGAGCTTG TTGAAGGGGA CTGTTCTCCC AGAGGCTTCT 
TTGCTCATCA CCACGAGGCC AACGGCTCTT CAAAGCCTGG GGCAGTGCCT GGAGGGGGAG 
TATTACGCAG AGATACTGGG ATTTTCAGCA GCCATGAGGG AGGAATATTT CCACAGATAT 
TTTGAGAATG CCAACAAAGC TGACATGGCC TTCAGGTTTG CCAGAGGCAA TGAGATCCTC 
TACAGCTTGT GCGTCATCCC CGTCATGAGC TGGGCTGTCT GCACCATCCT TGAACAAGAG 
CTTTGCAGGA AGAAAAACCT CCTTGAGTGC TCCAAAGCCA CCACAAGGAT GACCATGTCT 
TACCTCTCCC AGTTACTGAA GCACAGGGAC AGGGACAATC CACAGGTCCT CCAGAGGTTC 
CTTCTCCGGC TTTGCTCCTT GGCTGCCGAT GGCATCTGGA AGCACAAGGT CCTCTTTGAG 
GAGAAGGAGA TCAGGGACCG TGGCTTGGGC CAGCCAGACC TTCTTCCCCT CTTCCTCAAC 
GAGAAAATCT CAAAGGAAGG TGGAGACCAC GGGAAGGTCT ACGGCTTCAC CCATCTGCAC 
CTCCAGGAGT TTTTTGCAGC GATGTTTTAT GTTCTGGAGG ACGATGAGGA AACGGTCAGT 
GACTTGGGGG GGCTTGAGAA GGATGTAAGT AAGTTACTAG AAAGCTACAG TGAGTCCAGG 
AAGGATTTGA ATGTAACAGT GCAGTTCCTC TTTGGTTTAG TCAGCCAAAA ATCCATGGAG 
TATGTGGACA AAACCATTGG GTGCAGGATT TCACCACAAG CCAGGGAAGA CTTGCTGAAG 
TGGCTTCAGG GGAGGCACAG GAGCATCTCC CACCCCAGCC AAGCGCTGAA GATTTCGGAG 
TTGGACACTT TCCACTTCTT GTTCGAGATG AATGAGAAGA GCTTTGCACA AAGTGCGTTG 
GGTCATTTCA GTGAGCTGGA CTTGCAGGAC ATGAAGTTGA CTCTCTACGA CCAAGTGGCA 
CTTTCCTTCT GCATCCAGCA GTGGGCTGGG CTGGGCTGTG TCACCCTCCG GGGATGCTCC 
TTCCACCAGC AGGATCCTAG GGTGGAGCTG GATGCGTTGG TGCCCTGCTC TGGAGCCTCG 
GGAGCTGGAG GACGTCCTGG TGCCACCGCT GAGCTGCGGC AGGAGGAGCT GCACTGCCCC 
ATCCACCTGC TGTGCCGGGC ACTGCGGCAC CCAGGCAGTG CCCTGCGGGT GCTCCGGCTG 
CAGTGGTGCC GGCTGTCCGA GAGCTGCTGC GCCGAGCTGG CGGCGCTGCT GGCGGAACAC 
CCCAGCCTGG CGCAGCTGGA GCTGGGCGAC GGCGCGCTGG GCGACCGCGG CGTGCGCCTG 
CTGTGCCAGG GTCTGCGGCG TCCTGGCTGC CGCCTGCGCG TCCTGCGGCT GCGTTATTCC 
CGCCTCACCA GCAGCTGCTG TGAGGACCTC GCTGCCGTGC TGGGCACCAG CCCGTGGCTG 
GAGGAGCTGG ATTTGTCCTT CAGCGAGGGG CTGCGCGATG CCGGGGTGCA GCTGCTGTGC 
CAGGGGCTCC AGCACCGCGC CTGCCCGCTG CAGACGCTGC GGCTGGGGAG CTGCCGGCTG 
ACGGGCGCCT GCTGCCCAGC CCTGGCTGCA CAGCTGCTGG AGGGGCCTCA TCTCACCTGC 
CTGGACCTGA GTGACAACGA TCTGGGAGCT GATGGCATCC TGCATCTCTG CCAGCAGCTC 
CGGCATCCCG CCTGCCCACT GCGGACCCTG GGGCTGAGCA CGGATGGGTT AAGCCAGGAC 
GTGCTGCAGG AACTGGCCGC TCTCCAGGCC CTGAAGCCCG ATCTGAAGAT CAGGAACCTC 
CTGGAGCATG ATGTGCCAGA GGTGGGGGCC ATGGCCCGGC TGCCCTGCCA CCGTGGGGTC 
CAGCCAGGCG GCAGGAAGGC AATTCCCTCC TTCAGGAGAG CTCCAATCCT TTAG