akap1 cDNA ORF clone, Lates calcarifer(barramundi perch)

Gene
Clones
gRNAs

Summary

Gene Symbol	akap1
Entrez Gene ID	108899886
Full Name	A-kinase anchoring protein 1
Gene Type	protein-coding
Organism	Lates calcarifer(barramundi perch)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018700596.1	XP_018556112.1	A-kinase anchor protein 1, mitochondrial

Pathways from BioSystems
Gene Ontology

The following akap1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the akap1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLa47862	XM_018700596.1 Latest version!	Lates calcarifer A-kinase anchoring protein 1 (akap1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

akap1 ( XM_018700596.1 ) cDNA ORF clone, Lates calcarifer(barramundi perch) -> XP_018556112.1 Lates calcarifer A-kinase anchoring protein 1 (akap1), mRNA.

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CloneID	OLa47862
Clone ID Related Accession (Same CDS sequence)	XM_018700596.1
Accession Version	XM_018700596.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-13
Organism	Lates calcarifer(barramundi perch)
Product	A-kinase anchor protein 1, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017363841.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lates calcarifer Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACTGA GGTTTCGCTC TTTTGTACCG TACACACTGC CTGGAGTTCT TGCACTTATT 
GGCTGGTGGT GGTACATCTC ACGGAAGAGA GAGCGGCTCA TCAGTCATGA CAGCCCAGAC 
GGGGCTCAAA CTGCCATGGG CCTTAAAACA TCTCCAGCAG AGGGTAGCAA TGGCTTGGTT 
GAAAAAGGCA CTGTATCCTC CACAAATGAC ACAGAATGCC CCACCCACAG ACCTCCAAAG 
GTCACTAACC AGAGAACAGA ACATGAAAAT AATTCACATA TACATGTTCA GGGCAGTGAA 
GCCTCGCCTT CACTGAAACC AACTTCTGAA GAAGCTTCCC CTCACCTTGG CAGAACAAGA 
ACAGAGGAAC TCCTTAAACC ATTGGTCGTG ACCCTTCCAT CACCTGGCGA AGAAGACAGC 
CCGCAGGTGT CATTGAAACA CCAGAATGAC ACAGAAAAAA GCTCATCACT TGCTTTAGGA 
AGAGAGCTAG AGAAACACCA GATCAAAGAT TCAGCCTCTG CACCAGAGGC CTCAGAGGAG 
GTTATCTTGC CCTGTCACTC TAACAAAGAC TACCTCTCTG ATGCAGAGAG GCCTGAGCCA 
GAGGGAGAAG TTGCAAAGCA CAACAGTACA ATTAATACAC AAGATAAGAT TATTGTTTGC 
GCAGTCACTC CAGCTAAATT GCAGAGCGTG AACCCATCAG AATCTGACTC TCTTGAGACA 
CCCCCTACAG TGCAGGATTT CCACCAACAC ATTCTAACGA GCACACCTAC CACTCTGGCC 
CTGACCTCCA CAGCCTTGAC CATGGTCCAA GGCTCTCTCA CCAACTCCGT TACCCCAGGG 
GACATGCAGA TACACAATAG CAGTGGAAAG GAGCAGGATC TGGAGCTTCT GGCAGCTGGA 
CTCATATGCG AGGTCATCTC GGCAGCCACA CAGGAGGTCT TAGGTGTCAC CAGGTCCCAA 
ATCACAGATA ACAGTCAGCC CAGCTGCAGC AGCAGCACGC CCCCGGCTAG CAGCAGATTG 
TGCTCCCAAC AGGAGACATT CACAGCGGCA CAGCAGCACC ACCATCTACT GACAAGCTCT 
TCTCAGATAA GCTCTGAGTC TCCAGAGGCA ACAGAGAAAG AATGGGAAGG GATGCCTAAT 
GGATGTTCCT CAGCTCCAGT ATGGGAGGCT GCTGAGGTCA GTCATAGAGT TTGTCAGACA 
CACAATGCAC AGAGAGGCCA TTGGCCAACA ACATCACACG AAGCAGCACA AAGTATTCCT 
CTGCTTAACA TGAAACTGAA AGTTGATGAA GTGGCTTTGC TTGCTGAGGA CTCAGCCTGC 
AGCACATGCC ACTCTGAGGA TGGCATCAGC AGCGAGGACC TCCAGAACAG CATGTTTAAC 
AACCAAAGGG ATGTGATCCA AATTACAGAC TTGTCAGCAA GAGGAGCTGC ACAGCCTCAG 
TCACTAGTTG AGTCTGCCTC AGAGGCAATG GTTTTGGCTG CAACTGAAGA AAACTCAGTG 
GATGCTGTAT GTGAGATAAA GAGGCTCAAT GGAATAGGCC TGAGGAATGG AGCTCATGGG 
GCATGTGAGC TGGAGACAGA CCAGTCTGGA GGCTCTGATG TCAATAGTAT GGACTCAGTG 
GATAGTGGCT GCACTATGGG TGCTGGGGAA AGCCAGAGCA ACCACGCTGC CTCCTTGAGC 
TCAGAGCTCA TCATCTGGGA GATTGAGGTG CCAAAGCATC TTGTGGGGCG GTTAATCGGG 
AAGCAGGGGA GATTTGTGAG TTTCCTCAAG CAGAACTCTG GTGCAAAGAT CTACATCTCC 
ACCCTGCCTT ATACACAGGA GTTCCAGATC TGTCATATAG AAGGTACACA GCAGCAGGTT 
GACAAAGCCC TGTCATTGAT TGGAAAGAAG TTTAAAGATC TGGACTTGAC GAACTTGTAT 
GCACCTCCTC CGCCCCCACT CACGTTGCCA TCACTTCCCA TGACTTCCTG GCTTCTGCTT 
CCCAGTGGGG TGACAGTTGA AGTGATAGTG GTGAACATCG TTTCAGCCGG TCACGTCTTT 
GTCCAGCAGC ACACCCATCC CACCTACCAC GCGCTTCGAA GCCTTGACCA GCAGATGTTC 
ATCTGCTACT CCCAGCCAGG CACCCCTGCC TTGCCATCAC CTGCTGAAGT TGGTGTGATC 
TGTGCAGCTC CAGCAGTGGA AGGAGCCTGG TGGAGAGCTC AGGTCATCAC CTTCTATAAA 
GAGACAAATG AAGTGGAGAT TAGATACGTT GATTATGGGG GCTATGACAG AGTTAAGATT 
GACTCACTAC GGCAGATAAG GTCTGACTTT GTAACACTAC CATTTCAAGG TGCAGAAGTA 
TTGCTTGACA ACATTGCCCC ACTTCCAGGA GAGGATCGTT TTTCCCCTGA GGCCACATCA 
GCATTGGAGG AGATGGCCAG AGGAGTGGCA TTGCTTGCAC AGGTATCAAA CTATGATAAC 
AACACAGGCC TCCCACTGGT CCACCTGTGG AACATGGTCG GAGATGAGGT GATTTCAGTG 
AACCGTACAC TGGCAGAGAG AGGCCTGGGT ACTTGGGTGG ATGGATTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018556112.1
CDS	173..2743
Translation	MPLRFRSFVPYTLPGVLALIGWWWYISRKRERLISHDSPDGAQTAMGLKTSPAEGSNGLVEKGTVSSTNDTECPTHRPPKVTNQRTEHENNSHIHVQGSEASPSLKPTSEEASPHLGRTRTEELLKPLVVTLPSPGEEDSPQVSLKHQNDTEKSSSLALGRELEKHQIKDSASAPEASEEVILPCHSNKDYLSDAERPEPEGEVAKHNSTINTQDKIIVCAVTPAKLQSVNPSESDSLETPPTVQDFHQHILTSTPTTLALTSTALTMVQGSLTNSVTPGDMQIHNSSGKEQDLELLAAGLICEVISAATQEVLGVTRSQITDNSQPSCSSSTPPASSRLCSQQETFTAAQQHHHLLTSSSQISSESPEATEKEWEGMPNGCSSAPVWEAAEVSHRVCQTHNAQRGHWPTTSHEAAQSIPLLNMKLKVDEVALLAEDSACSTCHSEDGISSEDLQNSMFNNQRDVIQITDLSARGAAQPQSLVESASEAMVLAATEENSVDAVCEIKRLNGIGLRNGAHGACELETDQSGGSDVNSMDSVDSGCTMGAGESQSNHAASLSSELIIWEIEVPKHLVGRLIGKQGRFVSFLKQNSGAKIYISTLPYTQEFQICHIEGTQQQVDKALSLIGKKFKDLDLTNLYAPPPPPLTLPSLPMTSWLLLPSGVTVEVIVVNIVSAGHVFVQQHTHPTYHALRSLDQQMFICYSQPGTPALPSPAEVGVICAAPAVEGAWWRAQVITFYKETNEVEIRYVDYGGYDRVKIDSLRQIRSDFVTLPFQGAEVLLDNIAPLPGEDRFSPEATSALEEMARGVALLAQVSNYDNNTGLPLVHLWNMVGDEVISVNRTLAERGLGTWVDGF

Target ORF information:

RefSeq Version	XM_018700596.1
Organism	Lates calcarifer(barramundi perch)
Definition	Lates calcarifer A-kinase anchoring protein 1 (akap1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018700596.1

ORF Insert Sequence:

ATGCCACTGA GGTTTCGCTC TTTTGTACCG TACACACTGC CTGGAGTTCT TGCACTTATT 
GGCTGGTGGT GGTACATCTC ACGGAAGAGA GAGCGGCTCA TCAGTCATGA CAGCCCAGAC 
GGGGCTCAAA CTGCCATGGG CCTTAAAACA TCTCCAGCAG AGGGTAGCAA TGGCTTGGTT 
GAAAAAGGCA CTGTATCCTC CACAAATGAC ACAGAATGCC CCACCCACAG ACCTCCAAAG 
GTCACTAACC AGAGAACAGA ACATGAAAAT AATTCACATA TACATGTTCA GGGCAGTGAA 
GCCTCGCCTT CACTGAAACC AACTTCTGAA GAAGCTTCCC CTCACCTTGG CAGAACAAGA 
ACAGAGGAAC TCCTTAAACC ATTGGTCGTG ACCCTTCCAT CACCTGGCGA AGAAGACAGC 
CCGCAGGTGT CATTGAAACA CCAGAATGAC ACAGAAAAAA GCTCATCACT TGCTTTAGGA 
AGAGAGCTAG AGAAACACCA GATCAAAGAT TCAGCCTCTG CACCAGAGGC CTCAGAGGAG 
GTTATCTTGC CCTGTCACTC TAACAAAGAC TACCTCTCTG ATGCAGAGAG GCCTGAGCCA 
GAGGGAGAAG TTGCAAAGCA CAACAGTACA ATTAATACAC AAGATAAGAT TATTGTTTGC 
GCAGTCACTC CAGCTAAATT GCAGAGCGTG AACCCATCAG AATCTGACTC TCTTGAGACA 
CCCCCTACAG TGCAGGATTT CCACCAACAC ATTCTAACGA GCACACCTAC CACTCTGGCC 
CTGACCTCCA CAGCCTTGAC CATGGTCCAA GGCTCTCTCA CCAACTCCGT TACCCCAGGG 
GACATGCAGA TACACAATAG CAGTGGAAAG GAGCAGGATC TGGAGCTTCT GGCAGCTGGA 
CTCATATGCG AGGTCATCTC GGCAGCCACA CAGGAGGTCT TAGGTGTCAC CAGGTCCCAA 
ATCACAGATA ACAGTCAGCC CAGCTGCAGC AGCAGCACGC CCCCGGCTAG CAGCAGATTG 
TGCTCCCAAC AGGAGACATT CACAGCGGCA CAGCAGCACC ACCATCTACT GACAAGCTCT 
TCTCAGATAA GCTCTGAGTC TCCAGAGGCA ACAGAGAAAG AATGGGAAGG GATGCCTAAT 
GGATGTTCCT CAGCTCCAGT ATGGGAGGCT GCTGAGGTCA GTCATAGAGT TTGTCAGACA 
CACAATGCAC AGAGAGGCCA TTGGCCAACA ACATCACACG AAGCAGCACA AAGTATTCCT 
CTGCTTAACA TGAAACTGAA AGTTGATGAA GTGGCTTTGC TTGCTGAGGA CTCAGCCTGC 
AGCACATGCC ACTCTGAGGA TGGCATCAGC AGCGAGGACC TCCAGAACAG CATGTTTAAC 
AACCAAAGGG ATGTGATCCA AATTACAGAC TTGTCAGCAA GAGGAGCTGC ACAGCCTCAG 
TCACTAGTTG AGTCTGCCTC AGAGGCAATG GTTTTGGCTG CAACTGAAGA AAACTCAGTG 
GATGCTGTAT GTGAGATAAA GAGGCTCAAT GGAATAGGCC TGAGGAATGG AGCTCATGGG 
GCATGTGAGC TGGAGACAGA CCAGTCTGGA GGCTCTGATG TCAATAGTAT GGACTCAGTG 
GATAGTGGCT GCACTATGGG TGCTGGGGAA AGCCAGAGCA ACCACGCTGC CTCCTTGAGC 
TCAGAGCTCA TCATCTGGGA GATTGAGGTG CCAAAGCATC TTGTGGGGCG GTTAATCGGG 
AAGCAGGGGA GATTTGTGAG TTTCCTCAAG CAGAACTCTG GTGCAAAGAT CTACATCTCC 
ACCCTGCCTT ATACACAGGA GTTCCAGATC TGTCATATAG AAGGTACACA GCAGCAGGTT 
GACAAAGCCC TGTCATTGAT TGGAAAGAAG TTTAAAGATC TGGACTTGAC GAACTTGTAT 
GCACCTCCTC CGCCCCCACT CACGTTGCCA TCACTTCCCA TGACTTCCTG GCTTCTGCTT 
CCCAGTGGGG TGACAGTTGA AGTGATAGTG GTGAACATCG TTTCAGCCGG TCACGTCTTT 
GTCCAGCAGC ACACCCATCC CACCTACCAC GCGCTTCGAA GCCTTGACCA GCAGATGTTC 
ATCTGCTACT CCCAGCCAGG CACCCCTGCC TTGCCATCAC CTGCTGAAGT TGGTGTGATC 
TGTGCAGCTC CAGCAGTGGA AGGAGCCTGG TGGAGAGCTC AGGTCATCAC CTTCTATAAA 
GAGACAAATG AAGTGGAGAT TAGATACGTT GATTATGGGG GCTATGACAG AGTTAAGATT 
GACTCACTAC GGCAGATAAG GTCTGACTTT GTAACACTAC CATTTCAAGG TGCAGAAGTA 
TTGCTTGACA ACATTGCCCC ACTTCCAGGA GAGGATCGTT TTTCCCCTGA GGCCACATCA 
GCATTGGAGG AGATGGCCAG AGGAGTGGCA TTGCTTGCAC AGGTATCAAA CTATGATAAC 
AACACAGGCC TCCCACTGGT CCACCTGTGG AACATGGTCG GAGATGAGGT GATTTCAGTG 
AACCGTACAC TGGCAGAGAG AGGCCTGGGT ACTTGGGTGG ATGGATTTTG A