trmt1 cDNA ORF clone, Lates calcarifer(barramundi perch)

Gene
Clones
gRNAs

Summary

Gene Symbol	trmt1
Entrez Gene ID	108880839
Full Name	tRNA methyltransferase 1
Gene Type	protein-coding
Organism	Lates calcarifer(barramundi perch)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018672560.1	XP_018528076.1	tRNA (guanine(26)-N(2))-dimethyltransferase

Pathways from BioSystems
Gene Ontology

The following trmt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the trmt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLa83288	XM_018672560.1 Latest version!	Lates calcarifer tRNA methyltransferase 1 (trmt1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

trmt1 ( XM_018672560.1 ) cDNA ORF clone, Lates calcarifer(barramundi perch) -> XP_018528076.1 Lates calcarifer tRNA methyltransferase 1 (trmt1), mRNA.

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CloneID	OLa83288
Clone ID Related Accession (Same CDS sequence)	XM_018672560.1
Accession Version	XM_018672560.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-13
Organism	Lates calcarifer(barramundi perch)
Product	tRNA (guanine(26)-N(2))-dimethyltransferase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017367611.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lates calcarifer Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGGGC GGTCATCTTA CCTGTTCCCC CTCATCGTCT CCCATCACCT GCATCATTCT 
TGCACCTGTT CTGCTAGACC GACACTTCAG TGGAGGCTAC ACAGTGCTGC TGCTGCTGTA 
ATCAGCAGAG GACTGAAATC AATGGAGTCC CTAAAAGCCC TTCAGGACCC AGAGGTTCCC 
ACTACCAACC CTCCACCTAC GTCTGCTGGG TCTGCAGCAG ATGTTGTGAG CCCGGCCCCC 
TCAGACACAG CGTCTGATAA GGAGAAGACA CCCCCTGTGG GTCTTTTACC AGGGGAGACG 
GTGGTCAAGG AGGGCAAGGC GTCCATCTTG TTTCCTAGTG CAAATGAAGT GTTTTACAAC 
CCAGTACAGG AGTTCAACAG AGATTTGACA TGTGCTGTGA TCACAGAGTT TGCCAGAGAT 
CTGCTGGCTC AGCGTGGGGT GAAGGTGGTG GTGCCTGGAG AGCAAGAGAG GGTGGTGGTC 
TCCCTGTCGG AGGAGACAAA CGACGCTGAC ACGCAGATAG AGGAGAAAAA TGGAGCCGAG 
GAGCCAGCTG TAACTGCAAC AGTGGGGGAG AAATGTGAGC GTGGTCTTCG TGTCCTGGAG 
GGTTTGGCTG CGTCAGGTTT GCGCTCGGTA CGTTTTGCTC TGGAAGTCCC CGGCCTGCAC 
AGCGTCACCG CCAACGACTT CTCCACCAAG GCTGCGGCCC TGATCGCCAG GAACACCCAG 
TACAATGGAG TTAGCCACCT GCTCCAGGCC AGCTGCAGGG ATGCCAGCAT GCTGATGTAT 
GAGATGCGGG GGAAGAAGGA GCGGTATGAC GTCATCGATC TGGATCCCTA CGGTAGCCCT 
GCCCAATTCC TGGATGCCGC TGTACAGGCT GTCAGTGAAG GAGGTCTGTT GTGTGTAACA 
TGTACAGACA TGGCAGTGAT GGCAGGAAAC AGCGGAGAGA CCTGCTACAG CAAATACGGC 
TCAGTCTCCA TCAAAGCCAA ATACTGTCAT GAGATGGCTC TGCGTATCAT CCTCCACAGT 
TTGGACCAGA GGGCGGGGGT GTACCAGCGA TACATCCAGC CCCTGCTGTC TGTCAGTGTG 
GACTTTTACA TCCGAGTCTT TGTGCGTGTC TTCACAGGAC AGGCCAAAGT CAAAAACTCA 
GCCAGTAAAC AGGCTCTGGT CTACAACTGT GTCGGCTGTG GGTCTTTCTA CTTACAGAGA 
ATGGGCAGGA GAAAAAGCGA TGGAGGACAT ATGAAGTATA GTGCTGCCAC TGGACCCCCT 
GTTGGAACAG AGTGTGACCA CTGTGGACAC AAACATCAGA TGGGGGGTCC TATCTGGGCG 
GAGCCCATCC ACGACTTGGA GTTTGTCCAG AAGGTTTTAT CTGCTGTGTC GGGAAACCCA 
TCCCGATTTG GAACATCCAA GCGCATCAAC GGCATACTCA GCATGGTGAC TGAGGAGTTG 
GAAGATGTGC CTCTTTATTA CACTGTGGAA AATCTAAGCA GCGCAATACA CTGCAATACT 
CCATCCCTGC TCCAGTTTTG CTCTGCTCTT CTTCATGCTG GTCACAAGGT TTCCCTCTCT 
CACGCCTGTC AGACTGCCAT CAAGACAGAC GCTCCACCTG CCACCATCTG GGACATCATG 
CGATGCTGGG AGAAGGCTAA TCCTGTGAAG AGGGAGAAGC TGTCTAAGAC TAGCCCTGCC 
TTCAAGATCC TCTCCACTGA GCCCAGCCTC GAGGCCTGTT TCACTGTGAG GGAGGACGCC 
AACCCTCACT CCCGTAAACG CCACCTAAAC CGGTTCCAGG AGAATCCTCA GGCATTCTGG 
GGACCCAAAG CTCGTGCCAA ATCCAGCGGT GGCATCTCTA CTGACCTGCA GGACAAAAGA 
AAGAAGTTCC AGAACAAGAG GAAGAACCAG ACCACAGATC CCTCTCAGCT AAAAACCTTC 
CCCTGTAAGA GGTTCAAACA GGGATTATGC ACACATGGAG ACACATGCCG CTACTCCCAC 
GACCTGGAGC AGACAAAGGA AGAGAAAATG GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018528076.1
CDS	226..2241
Translation	MLGRSSYLFPLIVSHHLHHSCTCSARPTLQWRLHSAAAAVISRGLKSMESLKALQDPEVPTTNPPPTSAGSAADVVSPAPSDTASDKEKTPPVGLLPGETVVKEGKASILFPSANEVFYNPVQEFNRDLTCAVITEFARDLLAQRGVKVVVPGEQERVVVSLSEETNDADTQIEEKNGAEEPAVTATVGEKCERGLRVLEGLAASGLRSVRFALEVPGLHSVTANDFSTKAAALIARNTQYNGVSHLLQASCRDASMLMYEMRGKKERYDVIDLDPYGSPAQFLDAAVQAVSEGGLLCVTCTDMAVMAGNSGETCYSKYGSVSIKAKYCHEMALRIILHSLDQRAGVYQRYIQPLLSVSVDFYIRVFVRVFTGQAKVKNSASKQALVYNCVGCGSFYLQRMGRRKSDGGHMKYSAATGPPVGTECDHCGHKHQMGGPIWAEPIHDLEFVQKVLSAVSGNPSRFGTSKRINGILSMVTEELEDVPLYYTVENLSSAIHCNTPSLLQFCSALLHAGHKVSLSHACQTAIKTDAPPATIWDIMRCWEKANPVKREKLSKTSPAFKILSTEPSLEACFTVREDANPHSRKRHLNRFQENPQAFWGPKARAKSSGGISTDLQDKRKKFQNKRKNQTTDPSQLKTFPCKRFKQGLCTHGDTCRYSHDLEQTKEEKME

Target ORF information:

RefSeq Version	XM_018672560.1
Organism	Lates calcarifer(barramundi perch)
Definition	Lates calcarifer tRNA methyltransferase 1 (trmt1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018672560.1

ORF Insert Sequence:

ATGCTGGGGC GGTCATCTTA CCTGTTCCCC CTCATCGTCT CCCATCACCT GCATCATTCT 
TGCACCTGTT CTGCTAGACC GACACTTCAG TGGAGGCTAC ACAGTGCTGC TGCTGCTGTA 
ATCAGCAGAG GACTGAAATC AATGGAGTCC CTAAAAGCCC TTCAGGACCC AGAGGTTCCC 
ACTACCAACC CTCCACCTAC GTCTGCTGGG TCTGCAGCAG ATGTTGTGAG CCCGGCCCCC 
TCAGACACAG CGTCTGATAA GGAGAAGACA CCCCCTGTGG GTCTTTTACC AGGGGAGACG 
GTGGTCAAGG AGGGCAAGGC GTCCATCTTG TTTCCTAGTG CAAATGAAGT GTTTTACAAC 
CCAGTACAGG AGTTCAACAG AGATTTGACA TGTGCTGTGA TCACAGAGTT TGCCAGAGAT 
CTGCTGGCTC AGCGTGGGGT GAAGGTGGTG GTGCCTGGAG AGCAAGAGAG GGTGGTGGTC 
TCCCTGTCGG AGGAGACAAA CGACGCTGAC ACGCAGATAG AGGAGAAAAA TGGAGCCGAG 
GAGCCAGCTG TAACTGCAAC AGTGGGGGAG AAATGTGAGC GTGGTCTTCG TGTCCTGGAG 
GGTTTGGCTG CGTCAGGTTT GCGCTCGGTA CGTTTTGCTC TGGAAGTCCC CGGCCTGCAC 
AGCGTCACCG CCAACGACTT CTCCACCAAG GCTGCGGCCC TGATCGCCAG GAACACCCAG 
TACAATGGAG TTAGCCACCT GCTCCAGGCC AGCTGCAGGG ATGCCAGCAT GCTGATGTAT 
GAGATGCGGG GGAAGAAGGA GCGGTATGAC GTCATCGATC TGGATCCCTA CGGTAGCCCT 
GCCCAATTCC TGGATGCCGC TGTACAGGCT GTCAGTGAAG GAGGTCTGTT GTGTGTAACA 
TGTACAGACA TGGCAGTGAT GGCAGGAAAC AGCGGAGAGA CCTGCTACAG CAAATACGGC 
TCAGTCTCCA TCAAAGCCAA ATACTGTCAT GAGATGGCTC TGCGTATCAT CCTCCACAGT 
TTGGACCAGA GGGCGGGGGT GTACCAGCGA TACATCCAGC CCCTGCTGTC TGTCAGTGTG 
GACTTTTACA TCCGAGTCTT TGTGCGTGTC TTCACAGGAC AGGCCAAAGT CAAAAACTCA 
GCCAGTAAAC AGGCTCTGGT CTACAACTGT GTCGGCTGTG GGTCTTTCTA CTTACAGAGA 
ATGGGCAGGA GAAAAAGCGA TGGAGGACAT ATGAAGTATA GTGCTGCCAC TGGACCCCCT 
GTTGGAACAG AGTGTGACCA CTGTGGACAC AAACATCAGA TGGGGGGTCC TATCTGGGCG 
GAGCCCATCC ACGACTTGGA GTTTGTCCAG AAGGTTTTAT CTGCTGTGTC GGGAAACCCA 
TCCCGATTTG GAACATCCAA GCGCATCAAC GGCATACTCA GCATGGTGAC TGAGGAGTTG 
GAAGATGTGC CTCTTTATTA CACTGTGGAA AATCTAAGCA GCGCAATACA CTGCAATACT 
CCATCCCTGC TCCAGTTTTG CTCTGCTCTT CTTCATGCTG GTCACAAGGT TTCCCTCTCT 
CACGCCTGTC AGACTGCCAT CAAGACAGAC GCTCCACCTG CCACCATCTG GGACATCATG 
CGATGCTGGG AGAAGGCTAA TCCTGTGAAG AGGGAGAAGC TGTCTAAGAC TAGCCCTGCC 
TTCAAGATCC TCTCCACTGA GCCCAGCCTC GAGGCCTGTT TCACTGTGAG GGAGGACGCC 
AACCCTCACT CCCGTAAACG CCACCTAAAC CGGTTCCAGG AGAATCCTCA GGCATTCTGG 
GGACCCAAAG CTCGTGCCAA ATCCAGCGGT GGCATCTCTA CTGACCTGCA GGACAAAAGA 
AAGAAGTTCC AGAACAAGAG GAAGAACCAG ACCACAGATC CCTCTCAGCT AAAAACCTTC 
CCCTGTAAGA GGTTCAAACA GGGATTATGC ACACATGGAG ACACATGCCG CTACTCCCAC 
GACCTGGAGC AGACAAAGGA AGAGAAAATG GAATGA