ppef2 cDNA ORF clone, Lates calcarifer(barramundi perch)

Gene
Clones
gRNAs

Summary

Gene Symbol	ppef2
Entrez Gene ID	108874073
Full Name	protein phosphatase with EF-hand domain 2
Gene Type	protein-coding
Organism	Lates calcarifer(barramundi perch)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018662452.1	XP_018517968.1	LOW QUALITY PROTEIN: serine/threonine-protein phosphatase with EF-hands 2

Pathways from BioSystems
Gene Ontology

The following ppef2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ppef2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLa75624	XM_018662452.1 Latest version!	Lates calcarifer protein phosphatase with EF-hand domain 2 (ppef2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ppef2 ( XM_018662452.1 ) cDNA ORF clone, Lates calcarifer(barramundi perch) -> XP_018517968.1 Lates calcarifer protein phosphatase with EF-hand domain 2 (ppef2), mRNA.

Price & Availability↑

CloneID	OLa75624
Clone ID Related Accession (Same CDS sequence)	XM_018662452.1
Accession Version	XM_018662452.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2169bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-13
Organism	Lates calcarifer(barramundi perch)
Product	LOW QUALITY PROTEIN: serine/threonine-protein phosphatase with EF-hands 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017366727.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lates calcarifer Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 2 indels ##RefSeq-Attributes-END##

ATGGGATGTG GCTTTGGAAA ATCAGAACCA CAGCTGACGA AGTGTGACAG AGTGAGCATC 
TCAGTCCCAG CAATCCGAGC GGCTCTGTTG ATCCAGCGCT GGTACCGTCA GTATGTGGCC 
CGGCTGGAGA TGAGACGCAG GTGCACGTGG AACATCTTCC AGTCTATTGA ATATGCAGGA 
GAGCAAGACC AGATTAAGCT CTACAATTTT TTTGGCTTCC TGATGGACCA CTTCACCCCA 
GCCAGTAGTG AAAGAAACCT GATATCTCAC ATCTTTCGCG AGAATGAAAT CTGTCGTGAC 
ACAGAGTGGG AGAGATATTT TTGCTACAAG AGTGTCGAGG TACCTGACAG CTACACTGGC 
CCCCATCTGA CCTTCCCCAT GACCTTCTGT GGGGTATCGA AGCTGGTGGA GGCCTTCAAG 
CACAAACAAC AGCTCCATGC TCGATATGTT CTGCAGCTTC TTGGAGAGAC TTGGAGACTT 
CTAAGAATTC TCACAAATAT CAATCACGTT TCCACCTGCC CTATGAAGGA GATTACTATA 
TGTGGAGACT TACATGGGCA CCTTGAAGAC CTGCTGTTGA TATTCTATAA GAATGGTTTA 
CCATCTTCTG AGAAACCGTA CGTCTTCAAT GGAGACTTTG TGGATCGTGG CAAAAACTCC 
TTAGAGATCC TGCTTATCCT GTTTGGGTTC CTGCTGGTCT ATCCCAATGA TGTCCATCTG 
AACAGGGGAA ACCATGAGGA CCACATTGTT AACCTGAGAT ATGGTTTCAC TAAAGAAGTT 
CTGGGAAAAT ACAGGGTGCA CGGCAAGAAA ATCCTAAAGC TCCTGCAGAA GATCTTCAGC 
TGGCTGCCTC TGGCCACAAT GATAGATCAC AAGGTGCTGA TTGTGCATGG CGGCATCTCT 
GACACGACAG ACCTCGACAC AATAGCCAAA GTGGACAGAC ACAAATACGT GTCGGCTCTC 
AGGCCTCCTA AGCCGACCCA TCATGCAGTC AATGGCAGCA AGACTGTGAA TAACAACAAC 
ACTGAGGATG ACGGACTTGT GGGGGGCAGG CGTCGAGTGT ATTCTCTGAC TCACCATGGC 
TCGGCGCGGG CTCAAAGGCA CGATCCACCA CGCCGCTCGC TGCACAACCT GCCCATGAGC 
AGTCAGCTCA ACTTGTCAGT GGAAGAGGAG CTAAAGAGGA GGCGAAGGCT TGTTGGGTTC 
GACCAGTCCN NTATCGGGGA GGTGCAGAAG TCTGACCCTG ACTCAGACCC AGAGTCTGGA 
GAAACAGTGG AGACAAATGA GGATGAGTGG AAACAGATAG TGGATATGTT ATGGAGTGAT 
CCCATGCCCC AAAATGGCTG TGTTCCCAAT GAGGTGCGAG GTGGAGGCTG CTACTGGGGC 
CCAGATGTCA CTGAGGAAGT GCTGGGAAGA CACAACCTGC AGCTCCTCAT CCGCTCCCAT 
GAGTGCAAAC AGGACGGCTA TGAGTTCTGC CACAACCGCA GGGTGCTGAC TATATTTTCA 
GCATCTAACT ACTACGAGGT GGGCAGCAAC AGAGGAGCCT ACATCAGAAT GGGGCCTGAT 
CTGGTCCCAC ACTTCATTCA GTATCAGGCC AGCCGGACGA GCAGAGAGCT CACCCTGAGA 
CAAAGTGTTG GGTGGACAGA GAGATCAGCT CTGCGAGCTC TGAGAGAACA GCTGTTTGTA 
CATAAGTCAG ATCTCATCAG TGCCTTCCAG GAGTTTGATC CTAACAACAC AGGGCTCATC 
AGTCTAAGGG ACTGGGCCAG TGCCACAGAG AATGTACTGA AGCTGGGTCT GCCCTGGAGG 
GTGCTACGCT CTCAGCTTGT CAACAACACC CAGTATGGCA TGGTGAACTA CCAGCAGTGG 
ATAAGGGAGC TCTCCATCAC TGAGCCAAAA CTAGAGATAT CTGATACCAG CATCCTGGAG 
ACTATGTACA AAAACCACTC CAACCTGGAA ACCATCTTCC GAATCATAGA CACAGATCAC 
TCTGGTTTGA TCTCTTTCGA GGAGTTTCGT CAGACCTGGA AGCTCCTGTG CTCTCATCTT 
AAGATGGAGA TCAGTGATGA GGCCATCACA AACCTGGCCC AGAGCATTGA CTTCAACAAG 
GACGGGAGCA TCGACATCAA CGAGTTCATG GAGGCATTCC GGCTGGTAGA CCTCTCTGCG 
CATACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018517968.1
CDS	222..2390
Translation	MGCGFGKSEPQLTKCDRVSISVPAIRAALLIQRWYRQYVARLEMRRRCTWNIFQSIEYAGEQDQIKLYNFFGFLMDHFTPASSERNLISHIFRENEICRDTEWERYFCYKSVEVPDSYTGPHLTFPMTFCGVSKLVEAFKHKQQLHARYVLQLLGETWRLLRILTNINHVSTCPMKEITICGDLHGHLEDLLLIFYKNGLPSSEKPYVFNGDFVDRGKNSLEILLILFGFLLVYPNDVHLNRGNHEDHIVNLRYGFTKEVLGKYRVHGKKILKLLQKIFSWLPLATMIDHKVLIVHGGISDTTDLDTIAKVDRHKYVSALRPPKPTHHAVNGSKTVNNNNTEDDGLVGGRRRVYSLTHHGSARAQRHDPPRRSLHNLPMSSQLNLSVEEELKRRRRLVGFDQSnullIGEVQKSDPDSDPESGETVETNEDEWKQIVDMLWSDPMPQNGCVPNEVRGGGCYWGPDVTEEVLGRHNLQLLIRSHECKQDGYEFCHNRRVLTIFSASNYYEVGSNRGAYIRMGPDLVPHFIQYQASRTSRELTLRQSVGWTERSALRALREQLFVHKSDLISAFQEFDPNNTGLISLRDWASATENVLKLGLPWRVLRSQLVNNTQYGMVNYQQWIRELSITEPKLEISDTSILETMYKNHSNLETIFRIIDTDHSGLISFEEFRQTWKLLCSHLKMEISDEAITNLAQSIDFNKDGSIDINEFMEAFRLVDLSAHT

Target ORF information:

RefSeq Version	XM_018662452.1
Organism	Lates calcarifer(barramundi perch)
Definition	Lates calcarifer protein phosphatase with EF-hand domain 2 (ppef2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018662452.1

ORF Insert Sequence:

ATGGGATGTG GCTTTGGAAA ATCAGAACCA CAGCTGACGA AGTGTGACAG AGTGAGCATC 
TCAGTCCCAG CAATCCGAGC GGCTCTGTTG ATCCAGCGCT GGTACCGTCA GTATGTGGCC 
CGGCTGGAGA TGAGACGCAG GTGCACGTGG AACATCTTCC AGTCTATTGA ATATGCAGGA 
GAGCAAGACC AGATTAAGCT CTACAATTTT TTTGGCTTCC TGATGGACCA CTTCACCCCA 
GCCAGTAGTG AAAGAAACCT GATATCTCAC ATCTTTCGCG AGAATGAAAT CTGTCGTGAC 
ACAGAGTGGG AGAGATATTT TTGCTACAAG AGTGTCGAGG TACCTGACAG CTACACTGGC 
CCCCATCTGA CCTTCCCCAT GACCTTCTGT GGGGTATCGA AGCTGGTGGA GGCCTTCAAG 
CACAAACAAC AGCTCCATGC TCGATATGTT CTGCAGCTTC TTGGAGAGAC TTGGAGACTT 
CTAAGAATTC TCACAAATAT CAATCACGTT TCCACCTGCC CTATGAAGGA GATTACTATA 
TGTGGAGACT TACATGGGCA CCTTGAAGAC CTGCTGTTGA TATTCTATAA GAATGGTTTA 
CCATCTTCTG AGAAACCGTA CGTCTTCAAT GGAGACTTTG TGGATCGTGG CAAAAACTCC 
TTAGAGATCC TGCTTATCCT GTTTGGGTTC CTGCTGGTCT ATCCCAATGA TGTCCATCTG 
AACAGGGGAA ACCATGAGGA CCACATTGTT AACCTGAGAT ATGGTTTCAC TAAAGAAGTT 
CTGGGAAAAT ACAGGGTGCA CGGCAAGAAA ATCCTAAAGC TCCTGCAGAA GATCTTCAGC 
TGGCTGCCTC TGGCCACAAT GATAGATCAC AAGGTGCTGA TTGTGCATGG CGGCATCTCT 
GACACGACAG ACCTCGACAC AATAGCCAAA GTGGACAGAC ACAAATACGT GTCGGCTCTC 
AGGCCTCCTA AGCCGACCCA TCATGCAGTC AATGGCAGCA AGACTGTGAA TAACAACAAC 
ACTGAGGATG ACGGACTTGT GGGGGGCAGG CGTCGAGTGT ATTCTCTGAC TCACCATGGC 
TCGGCGCGGG CTCAAAGGCA CGATCCACCA CGCCGCTCGC TGCACAACCT GCCCATGAGC 
AGTCAGCTCA ACTTGTCAGT GGAAGAGGAG CTAAAGAGGA GGCGAAGGCT TGTTGGGTTC 
GACCAGTCCN NTATCGGGGA GGTGCAGAAG TCTGACCCTG ACTCAGACCC AGAGTCTGGA 
GAAACAGTGG AGACAAATGA GGATGAGTGG AAACAGATAG TGGATATGTT ATGGAGTGAT 
CCCATGCCCC AAAATGGCTG TGTTCCCAAT GAGGTGCGAG GTGGAGGCTG CTACTGGGGC 
CCAGATGTCA CTGAGGAAGT GCTGGGAAGA CACAACCTGC AGCTCCTCAT CCGCTCCCAT 
GAGTGCAAAC AGGACGGCTA TGAGTTCTGC CACAACCGCA GGGTGCTGAC TATATTTTCA 
GCATCTAACT ACTACGAGGT GGGCAGCAAC AGAGGAGCCT ACATCAGAAT GGGGCCTGAT 
CTGGTCCCAC ACTTCATTCA GTATCAGGCC AGCCGGACGA GCAGAGAGCT CACCCTGAGA 
CAAAGTGTTG GGTGGACAGA GAGATCAGCT CTGCGAGCTC TGAGAGAACA GCTGTTTGTA 
CATAAGTCAG ATCTCATCAG TGCCTTCCAG GAGTTTGATC CTAACAACAC AGGGCTCATC 
AGTCTAAGGG ACTGGGCCAG TGCCACAGAG AATGTACTGA AGCTGGGTCT GCCCTGGAGG 
GTGCTACGCT CTCAGCTTGT CAACAACACC CAGTATGGCA TGGTGAACTA CCAGCAGTGG 
ATAAGGGAGC TCTCCATCAC TGAGCCAAAA CTAGAGATAT CTGATACCAG CATCCTGGAG 
ACTATGTACA AAAACCACTC CAACCTGGAA ACCATCTTCC GAATCATAGA CACAGATCAC 
TCTGGTTTGA TCTCTTTCGA GGAGTTTCGT CAGACCTGGA AGCTCCTGTG CTCTCATCTT 
AAGATGGAGA TCAGTGATGA GGCCATCACA AACCTGGCCC AGAGCATTGA CTTCAACAAG 
GACGGGAGCA TCGACATCAA CGAGTTCATG GAGGCATTCC GGCTGGTAGA CCTCTCTGCG 
CATACCTGA